Medical antibacterial dressing and preparation method thereof

Abstract

The invention discloses a medical antibacterial dressing and a preparation method thereof, and relates to the technical field of medical materials, the dressing is formed by blending chitosan composite fibers, rose fibers, bamboo fibers and antibacterial nanofibers; the weight ratio of the chitosan composite fibers to the rose fibers to the bamboo fibers to the antibacterial nanofibers is 1: (0.2-0.4): 0.3: 0.5. The medical antibacterial dressing disclosed by the invention is good in antibacterial property and remarkable in wound healing promoting effect.

Description

Technical Field

[0001] The invention relates to the technical field of medical materials, and in particular to a medical antibacterial dressing and a preparation method thereof. Background Art

[0002] Medical dressings are medical materials that directly cover the damaged surface of organisms such as sores and wounds, and play a basic protective role on the surface of organisms. They can provide an ideal environment for wound healing, inhibit bacteria, promote wound healing, and relieve wound pain. With the advancement of medical science, the demand for medical dressings is becoming more and more extensive, and the requirements for their performance are also getting higher and higher.

[0003] At present, the most commonly used medical dressings in clinical practice are mainly gauze, cotton pads, etc., which are mostly made of cotton, soft linen and linen. They are inert dressings and have no obvious effect on wound healing. This kind of dressing is cheap and widely available.

[0004] It is easy to make, has a certain degree of absorption of wound exudate, can protect the wound, and can be reused. However, with the continuous deepening of research on wound repair, this type of medical dressing is increasingly showing its limitations due to its disadvantages of not being conducive to wound healing, easily causing wound infection, and poor hemostasis. Other types of medical dressings on the market also have more or less technical defects such as insufficient antibacterial properties and ineffective wound healing.

[0005] In order to solve the above problems, a Chinese invention patent with authorization announcement number CN113318260B discloses a medical antibacterial dressing and a preparation method thereof, which comprises the following steps: after laying a laminated structure in the order of covering adhesive layer, hot-melt mesh adhesive, porous adsorption material, hot-melt mesh adhesive and covering adhesive layer, directly obtaining a composite structure of covering adhesive layer / mesh adhesive / porous adsorption layer / mesh adhesive / covering adhesive layer by hot pressing; then cutting the composite structure into a plurality of strips of a certain size, and removing the covering adhesive layer to obtain a composite structure of mesh adhesive / porous adsorption layer / mesh adhesive; finally, bonding a medical tape to one side of the composite structure of mesh adhesive / porous adsorption layer / mesh adhesive, bonding a covering adhesive layer whose size matches that of the medical tape to the other side of the composite structure, and performing irradiation sterilization after overall packaging to prepare a medical antibacterial dressing. Compared with the prior art, the medical antibacterial dressing of the invention has a high antibacterial rate, far infrared function, high effective adsorption capacity retention rate, good air permeability, good moisture permeability, good flexibility, excellent deodorization function, and strong absorption and penetration capacity. However, its antibacterial property and wound healing effect still need to be further improved. Summary of the invention

[0006] In order to overcome the defects in the prior art, the present invention provides a medical antibacterial dressing with good antibacterial property and significant effect of promoting wound healing and a preparation method thereof.

[0007] The technical solution adopted by the present invention to solve the technical problem is: a medical antibacterial dressing, which is blended by chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber; the weight ratio of the chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber is 1: (0.2-0.4): 0.3: 0.5; the preparation method of the antibacterial nanofiber comprises the following steps:

[0008] Step D1, adding acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, and an initiator to a high boiling point solvent, stirring and reacting at 55-75° C. in an inert gas atmosphere for 4-6 hours, then precipitating in water, washing the precipitated polymer with ethanol 3-6 times, and finally drying in a vacuum drying oven at 85-95° C. to constant weight to obtain an antibacterial copolymer;

[0009] Step D2, adding the antibacterial copolymer prepared in step D1 to 2,2,2-trifluoroethanol, and mixing them uniformly to form a dispersion A; adding hyperbranched polylysine to deionized water, and mixing them uniformly to form a dispersion B; mixing the dispersion A and the dispersion B to obtain a spinning solution;

[0010] Step D3, electrospinning the spinning solution to obtain raw fibers;

[0011] Step D4: dispersing sodium alginate in water to form a modifier, spraying the modifier on the surface of the original fiber, and then post-processing to obtain antibacterial nanofibers.

[0012] Preferably, the mass ratio of acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, initiator and high boiling point solvent in step D1 is 1:(0.8-1.2):(3-5):(0.05-0.08):(20-35).

[0013] Preferably, there is no special requirement for the source of the acrylamide β-cyclodextrin quaternary ammonium salt. In one embodiment of the present invention, the acrylamide β-cyclodextrin quaternary ammonium salt is prepared according to the preparation method of reactive β-cyclodextrin quaternary ammonium salt in Example 6 of Chinese invention patent with authorization announcement number CN106008755B.

[0014] Preferably, the initiator is azobisisobutyronitrile; the high boiling point solvent is at least one of dimethyl sulfoxide, N,N-dimethylformamide, and N-methylpyrrolidone; and the inert gas is any one of nitrogen, helium, neon, and argon.

[0015] Preferably, the mass ratio of the antibacterial copolymer and 2,2,2-trifluoroethanol in step D2 is (1.2-2.2):10.

[0016] Preferably, the mass ratio of the hyperbranched polylysine to deionized water in step D2 is (1-3):10.

[0017] Preferably, there is no special requirement for the source of the hyperbranched lysine. In one embodiment of the present invention, the hyperbranched lysine is prepared according to the method of Example 1 of the Chinese invention patent application publication number CN117205361A.

[0018] Preferably, the mass ratio of dispersion A to dispersion B in step D2 is 10:(0.4-1.6).

[0019] Preferably, the process parameters of the electrospinning in step D3 include: a voltage of 15-35 kV, and a distance between the spinneret and the receiver of 12-30 cm.

[0020] Preferably, in step D4, the mass ratio of sodium alginate to water is 1:(4-6); the mass ratio of sodium alginate to fibrils is (1-2):10.

[0021] Preferably, the post-treatment in step D4 is specifically: vacuum drying for 5-10 hours under the conditions of vacuum gauge pressure of -0.05 to -0.15 MPa and temperature of 80-90°C.

[0022] Preferably, there is no special requirement for the source of the chitosan composite fiber. In one embodiment of the present invention, the chitosan composite fiber is made according to the method of Example 9 of the Chinese invention patent with authorization announcement number CN108914248B.

[0023] Preferably, the rose fiber is Rose fiber viscose 32S is provided by Shaoxing Xineng Textile Technology Co., Ltd.; the fiber fineness of the bamboo fiber is 6D.

[0024] Another object of the present invention is to provide a method for preparing the medical antibacterial dressing, comprising the following steps: blending chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber into a dressing blank, then rolling it through a rolling mill, and then cutting it into 5×5 cm squares, and packaging it to obtain the medical antibacterial dressing.

[0025] Preferably, the temperature of the rolling mill during rolling is 115° C.-155° C., and the speed at which the grey cloth passes through the hot rollers of the rolling mill is 130-320 rpm.

[0026] The beneficial effects of adopting the above technical solution are:

[0027] (1) The preparation method of the medical antibacterial dressing provided by the present invention has a short process flow, simple operation, high preparation efficiency and finished product qualification rate, is easy to promote and use, and the product can be industrialized.

[0028] (2) The medical antibacterial dressing provided by the present invention is blended from chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber, and combines the advantages of these fiber materials, so that the prepared medical antibacterial dressing has good antibacterial properties and a significant effect on promoting wound healing; the antibacterial nanofiber is made of acrylamide-based β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethylmethylacrylamide and hyperbranched polylysine as raw materials through electrostatic spinning, and cyclodextrin quaternary ammonium salt, thiazole, hydroxyl and hyperbranched polylysine structures are simultaneously introduced into the fiber material structure. Under the multiple effects of these structures or groups such as electronic effect, steric effect and conjugation effect, the prepared medical antibacterial dressing has better antibacterial and wound healing effects, more stable performance, and can provide a comfortable healing environment for the wound.

[0029] (3) The medical antibacterial dressing provided by the present invention has good compatibility with the skin, low skin irritation, obvious antibacterial effect, significant effect in promoting wound healing, long shelf life and sufficient stability through the reasonable selection of antibacterial nanofibers and dressing preparation process parameters. DETAILED DESCRIPTION

[0030] In order to make the technical solution of the present invention better understood by the technical personnel in the field, and to make the above-mentioned features, purposes and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with the embodiments. The embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0031] Example 1

[0032] A medical antibacterial dressing, which is blended from chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber; the weight ratio of the chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber is 1:0.2:0.3:0.5; the preparation method of the antibacterial nanofiber comprises the following steps:

[0033] Step D1, adding acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, and an initiator to a high boiling point solvent, stirring and reacting at 55° C. in an inert gas atmosphere for 4 hours, then precipitating in water, washing the precipitated polymer with ethanol 3 times, and finally drying in a vacuum drying oven at 85° C. to constant weight to obtain an antibacterial copolymer; the acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, and an initiator in step D1 are -trimethylol methacrylamide, initiator, high boiling point solvent mass ratio is 1:0.8:3:0.05:20; the acrylamide β-cyclodextrin quaternary ammonium salt is prepared according to the preparation method of reactive β-cyclodextrin quaternary ammonium salt in Example 6 of Chinese invention patent with authorization announcement number CN106008755B; the initiator is azobisisobutyronitrile; the high boiling point solvent is dimethyl sulfoxide; the inert gas is nitrogen. Through GPC test, the M of the obtained functional polymer is measured. n =16381 g / mol, M W / M n =1.358; through elemental analysis, the mass ratio of the structural units introduced by acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, and N-trimethylolmethyl acrylamide is 0.98:0.79:2.99;

[0034] Step D2, adding the antibacterial copolymer prepared in step D1 to 2,2,2-trifluoroethanol, and mixing them uniformly to form a dispersion A; adding hyperbranched polylysine to deionized water, and mixing them uniformly to form a dispersion B; mixing the dispersion A and the dispersion B to obtain a spinning solution;

[0035] Step D3, electrospinning the spinning solution to obtain raw fibers;

[0036] Step D4: dispersing sodium alginate in water to form a modifier, spraying the modifier on the surface of the original fiber, and then post-processing to obtain antibacterial nanofibers.

[0037] The mass ratio of the antibacterial copolymer and 2,2,2-trifluoroethanol in step D2 is 1.2:10; the mass ratio of the hyperbranched polylysine and deionized water in step D2 is 1:10; the hyperbranched lysine is prepared according to the method of Example 1 of the Chinese invention patent with application publication number CN117205361A; the mass ratio of the dispersion A and the dispersion B in step D2 is 10:0.4.

[0038] The process parameters of the electrospinning in step D3 include: a voltage of 15 kV, a distance between the spinneret and the receiver of 12 cm; in step D4, the mass ratio of sodium alginate to water is 1:4; and the mass ratio of sodium alginate to fibrils is 1:10.

[0039] The post-treatment in step D4 is specifically: vacuum drying for 5 hours at a vacuum gauge pressure of -0.05 MPa and a temperature of 80°C.

[0040] The chitosan composite fiber is made according to the method of Example 9 of the Chinese invention patent with the authorization announcement number CN108914248B; the rose fiber is Rose fiber viscose 32S is provided by Shaoxing Xineng Textile Technology Co., Ltd.; the fiber fineness of the bamboo fiber is 6D.

[0041] A preparation method of the medical antibacterial dressing comprises the following steps: chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber are blended to form dressing grey cloth, which is then rolled by a rolling mill, cut into 5×5 cm squares, and packaged to obtain the medical antibacterial dressing; the rolling temperature of the rolling mill is 115° C., and the speed of the grey cloth passing through the hot roller of the rolling mill is 130 rpm.

[0042] Example 2

[0043] A medical antibacterial dressing, which is blended from chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber; the weight ratio of the chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber is 1:0.25:0.3:0.5; the preparation method of the antibacterial nanofiber comprises the following steps:

[0044] Step D1, adding acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, and an initiator to a high boiling point solvent, stirring and reacting at 60° C. in an inert gas atmosphere for 4.5 hours, then precipitating in water, washing the precipitated polymer with ethanol 4 times, and finally drying in a vacuum drying oven at 87° C. to constant weight to obtain an antibacterial copolymer;

[0045] Step D2, adding the antibacterial copolymer prepared in step D1 to 2,2,2-trifluoroethanol, and mixing them uniformly to form a dispersion A; adding hyperbranched polylysine to deionized water, and mixing them uniformly to form a dispersion B; mixing the dispersion A and the dispersion B to obtain a spinning solution;

[0046] Step D3, electrospinning the spinning solution to obtain raw fibers;

[0047] Step D4: dispersing sodium alginate in water to form a modifier, spraying the modifier on the surface of the original fiber, and then post-processing to obtain antibacterial nanofibers.

[0048] The mass ratio of acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, initiator, and high boiling point solvent in step D1 is 1:0.9:3.5:0.06:25; the acrylamide β-cyclodextrin quaternary ammonium salt is prepared according to the preparation method of reactive β-cyclodextrin quaternary ammonium salt in Example 6 of Chinese invention patent with authorization announcement number CN106008755B; the initiator is azobisisobutyronitrile; the high boiling point solvent is N,N-dimethylformamide; and the inert gas is helium.

[0049] The mass ratio of the antibacterial copolymer and 2,2,2-trifluoroethanol in step D2 is 1.5:10; the mass ratio of the hyperbranched polylysine and deionized water in step D2 is 1.5:10; the hyperbranched lysine is prepared according to the method of Example 1 of the Chinese invention patent with application publication number CN117205361A; the mass ratio of the dispersion A and the dispersion B in step D2 is 10:0.8.

[0050] The process parameters of the electrospinning described in step D3 include: a voltage of 20 kV, a distance between the spinneret and the receiver of 16 cm; the mass ratio of sodium alginate to water described in step D4 is 1:4.5; the mass ratio of sodium alginate to fibril is 1.2:10; the post-treatment described in step D4 is specifically: vacuum drying for 6 hours under the conditions of vacuum gauge pressure of -0.07 MPa and temperature of 83°C.

[0051] The chitosan composite fiber is made according to the method of Example 9 of the Chinese invention patent with the authorization announcement number CN108914248B; the rose fiber is Rose fiber viscose 32S is provided by Shaoxing Xineng Textile Technology Co., Ltd.; the fiber fineness of the bamboo fiber is 6D.

[0052] A preparation method of the medical antibacterial dressing comprises the following steps: chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber are blended to form dressing grey cloth, which is then rolled by a rolling mill, cut into 5×5 cm squares, and packaged to obtain the medical antibacterial dressing; the rolling temperature of the rolling mill is 125° C., and the speed of the grey cloth passing through the hot roller of the rolling mill is 170 rpm.

[0053] Example 3

[0054] A medical antibacterial dressing, which is blended from chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber; the weight ratio of the chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber is 1:0.3:0.3:0.5; the preparation method of the antibacterial nanofiber comprises the following steps:

[0055] Step D1, adding acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, and an initiator to a high boiling point solvent, stirring and reacting at 65° C. in an inert gas atmosphere for 5 hours, then precipitating in water, washing the precipitated polymer with ethanol 5 times, and finally drying in a vacuum drying oven at 90° C. to constant weight to obtain an antibacterial copolymer;

[0056] Step D2, adding the antibacterial copolymer prepared in step D1 to 2,2,2-trifluoroethanol, and mixing them uniformly to form a dispersion A; adding hyperbranched polylysine to deionized water, and mixing them uniformly to form a dispersion B; mixing the dispersion A and the dispersion B to obtain a spinning solution;

[0057] Step D3, electrospinning the spinning solution to obtain raw fibers;

[0058] Step D4: dispersing sodium alginate in water to form a modifier, spraying the modifier on the surface of the original fiber, and then post-processing to obtain antibacterial nanofibers.

[0059] The mass ratio of acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, initiator and high boiling point solvent in step D1 is 1:1:4:0.065:28; the acrylamide β-cyclodextrin quaternary ammonium salt is prepared according to the preparation method of reactive β-cyclodextrin quaternary ammonium salt in Example 6 of Chinese invention patent with authorization announcement number CN106008755B; the initiator is azobisisobutyronitrile; the high boiling point solvent is N-methylpyrrolidone; and the inert gas is neon.

[0060] The mass ratio of the antibacterial copolymer and 2,2,2-trifluoroethanol in step D2 is 1.8:10; the mass ratio of the hyperbranched polylysine and deionized water in step D2 is 2:10; the hyperbranched lysine is prepared according to the method of Example 1 of the Chinese invention patent with application publication number CN117205361A; the mass ratio of the dispersion A and the dispersion B in step D2 is 10:1.

[0061] The process parameters of the electrospinning described in step D3 include: a voltage of 28 kV, a distance between the spinneret and the receiver of 23 cm; the mass ratio of sodium alginate to water described in step D4 is 1:5; the mass ratio of sodium alginate to fibril is 1.5:10; the post-treatment described in step D4 is specifically: vacuum drying for 7.5 hours under the conditions of vacuum gauge pressure of -0.1 MPa and temperature of 85°C.

[0062] The chitosan composite fiber is made according to the method of Example 9 of the Chinese invention patent with the authorization announcement number CN108914248B; the rose fiber is Rose fiber viscose 32S is provided by Shaoxing Xineng Textile Technology Co., Ltd.; the fiber fineness of the bamboo fiber is 6D.

[0063] A preparation method of the medical antibacterial dressing comprises the following steps: chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber are blended to form dressing grey cloth, which is then rolled by a rolling mill, cut into 5×5 cm squares, and packaged to obtain the medical antibacterial dressing; the rolling temperature of the rolling mill is 135° C., and the speed of the grey cloth passing through the hot roller of the rolling mill is 230 rpm.

[0064] Example 4

[0065] A medical antibacterial dressing, which is blended from chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber; the weight ratio of the chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber is 1:0.35:0.3:0.5; the preparation method of the antibacterial nanofiber comprises the following steps:

[0066] Step D1, adding acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, and an initiator to a high boiling point solvent, stirring and reacting at 70° C. in an inert gas atmosphere for 5.5 hours, then precipitating in water, washing the precipitated polymer with ethanol 6 times, and finally drying in a vacuum drying oven at 93° C. to constant weight to obtain an antibacterial copolymer;

[0067] Step D2, adding the antibacterial copolymer prepared in step D1 to 2,2,2-trifluoroethanol, and mixing them uniformly to form a dispersion A; adding hyperbranched polylysine to deionized water, and mixing them uniformly to form a dispersion B; mixing the dispersion A and the dispersion B to obtain a spinning solution;

[0068] Step D3, electrospinning the spinning solution to obtain raw fibers;

[0069] Step D4: dispersing sodium alginate in water to form a modifier, spraying the modifier on the surface of the original fiber, and then post-processing to obtain antibacterial nanofibers.

[0070] The mass ratio of acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, initiator and high boiling point solvent in step D1 is 1:1.1:4.5:0.075:33; the acrylamide β-cyclodextrin quaternary ammonium salt is prepared according to the preparation method of reactive β-cyclodextrin quaternary ammonium salt in Example 6 of Chinese invention patent with authorization announcement number CN106008755B; the initiator is azobisisobutyronitrile; the high boiling point solvent is a mixture of dimethyl sulfoxide, N,N-dimethylformamide and N-methylpyrrolidone in a mass ratio of 1:1:2; and the inert gas is argon.

[0071] The mass ratio of the antibacterial copolymer and 2,2,2-trifluoroethanol in step D2 is 2:10; the mass ratio of the hyperbranched polylysine and deionized water in step D2 is 2.5:10; the hyperbranched lysine is prepared according to the method of Example 1 of the Chinese invention patent with application publication number CN117205361A; the mass ratio of the dispersion A and the dispersion B in step D2 is 10:1.4; the process parameters of the electrospinning in step D3 include: a voltage of 33 kV and a distance of 28 cm between the spinneret and the receiver; the mass ratio of sodium alginate and water in step D4 is 1:5.5; the mass ratio of sodium alginate and fibrils is 1.8:10; the post-treatment in step D4 is specifically: vacuum drying for 9 hours under a vacuum gauge pressure of -0.13 MPa and a temperature of 88°C.

[0072] The chitosan composite fiber is made according to the method of Example 9 of the Chinese invention patent with the authorization announcement number CN108914248B; the rose fiber is Rose fiber viscose 32S is provided by Shaoxing Xineng Textile Technology Co., Ltd.; the fiber fineness of the bamboo fiber is 6D.

[0073] A preparation method of the medical antibacterial dressing comprises the following steps: chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber are blended to form dressing grey cloth, which is then rolled by a rolling mill, cut into 5×5 cm squares, and packaged to obtain the medical antibacterial dressing; the rolling temperature of the rolling mill is 145° C., and the speed of the grey cloth passing through the hot roller of the rolling mill is 300 rpm.

[0074] Example 5

[0075] A medical antibacterial dressing, which is blended from chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber; the weight ratio of the chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber is 1:0.4:0.3:0.5; the preparation method of the antibacterial nanofiber comprises the following steps:

[0076] Step D1, adding acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, and an initiator to a high boiling point solvent, stirring and reacting at 75° C. in an inert gas atmosphere for 6 hours, then precipitating in water, washing the precipitated polymer with ethanol 6 times, and finally drying in a vacuum drying oven at 95° C. to constant weight to obtain an antibacterial copolymer;

[0077] Step D2, adding the antibacterial copolymer prepared in step D1 to 2,2,2-trifluoroethanol, and mixing them uniformly to form a dispersion A; adding hyperbranched polylysine to deionized water, and mixing them uniformly to form a dispersion B; mixing the dispersion A and the dispersion B to obtain a spinning solution;

[0078] Step D3, electrospinning the spinning solution to obtain raw fibers;

[0079] Step D4: dispersing sodium alginate in water to form a modifier, spraying the modifier on the surface of the original fiber, and then post-processing to obtain antibacterial nanofibers.

[0080] The mass ratio of acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, initiator and high boiling point solvent in step D1 is 1:1.2:5:0.08:35; the acrylamide β-cyclodextrin quaternary ammonium salt is prepared according to the preparation method of reactive β-cyclodextrin quaternary ammonium salt in Example 6 of Chinese invention patent with authorization announcement number CN106008755B; the initiator is azobisisobutyronitrile; the high boiling point solvent is dimethyl sulfoxide; and the inert gas is nitrogen.

[0081] The mass ratio of the antibacterial copolymer and 2,2,2-trifluoroethanol in step D2 is 2.2:10; the mass ratio of the hyperbranched polylysine and deionized water in step D2 is 3:10; the hyperbranched lysine is prepared according to the method of Example 1 of the Chinese invention patent with application publication number CN117205361A; the mass ratio of the dispersion A and the dispersion B in step D2 is 10:1.6; the process parameters of the electrospinning in step D3 include: a voltage of 35 kV and a distance of 30 cm between the spinneret and the receiver; the mass ratio of sodium alginate and water in step D4 is 1:6; the mass ratio of sodium alginate and fibrils is 2:10; the post-treatment in step D4 is specifically: vacuum drying for 10 hours under a vacuum gauge pressure of -0.15 MPa and a temperature of 90°C.

[0082] The chitosan composite fiber is made according to the method of Example 9 of the Chinese invention patent with the authorization announcement number CN108914248B; the rose fiber is Rose fiber viscose 32S is provided by Shaoxing Xineng Textile Technology Co., Ltd.; the fiber fineness of the bamboo fiber is 6D.

[0083] A preparation method of the medical antibacterial dressing comprises the following steps: chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber are blended to form dressing grey cloth, which is then rolled by a rolling mill, cut into 5×5 cm squares, and packaged to obtain the medical antibacterial dressing; the rolling temperature of the rolling mill is 155° C., and the speed of the grey cloth passing through the hot roller of the rolling mill is 320 rpm.

[0084] Comparative Example 1

[0085] A medical antibacterial dressing, which is substantially the same as Example 1, except that 4-methyl-5-vinylthiazole is not added, and an equal amount of bamboo fiber is used instead of rose fiber.

[0086] Comparative Example 2

[0087] A medical antibacterial dressing is substantially the same as Example 1, except that no acrylamide-based β-cyclodextrin quaternary ammonium salt is added, and an equal amount of rose fiber is used instead of bamboo fiber.

[0088] The performance of the medical antibacterial dressings described in Examples 1-5 and Comparative Examples 1-2 was tested. The test results are shown in Table 1. The test method is as follows:

[0089] (1) Antibacterial property: The antibacterial rate was tested according to GB / T 20944.3-2008.

[0090] (2) Animal wound healing experiment: 84 rabbits (in good health and weighing 2-3 kg) were divided into 7 groups (Group A, Group B, Group C, Group D, Group E, Group F, and Group G), with 12 rabbits in each group. A 5 cm × 5 cm area was reserved on the back of the rabbits to remove the dorsal hair. After disinfection, a 1 cm × 1 cm wound was made on the back with a scalpel. The wounds were treated with the dressings prepared in Examples 1-5 and Comparative Examples 1-2 of the present invention, respectively. The dressings were changed twice a day. After 7 days, the wound healing conditions of the rabbits were statistically recorded, wherein wound healing rate (%) = (original wound area - unhealed wound area) / original wound area × 100%. The average wound healing rate of each group of rabbits was taken as the animal wound healing rate of the product of this example.

[0091] Table 1

[0092]

[0093] It can be seen from Table 1 that the medical antibacterial dressing disclosed in the embodiment of the present invention has better antibacterial properties and wound healing effects than the comparative example; the combined use of 4-methyl-5-vinylthiazole, acrylamide-based β-cyclodextrin quaternary ammonium salt, bamboo fiber and rose fiber is beneficial to improving the above properties.

[0094] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions only describe the principles of the present invention. The present invention may be subject to various changes and improvements without departing from the spirit and scope of the present invention. These changes and improvements fall within the scope of the present invention. The scope of protection claimed by the present invention is defined by the attached claims and their equivalents.

Claims

1. A medical antibacterial dressing, characterized in that: The dressing is blended by chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber; the weight ratio of the chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber is 1:(0.2-0.4):0.3:0.5; the preparation method of the antibacterial nanofiber comprises the following steps: Step D1, adding acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, and an initiator to a high boiling point solvent, stirring and reacting at 55-75° C. in an inert gas atmosphere for 4-6 hours, then precipitating in water, washing the precipitated polymer with ethanol 3-6 times, and finally drying in a vacuum drying oven at 85-95° C. to constant weight to obtain an antibacterial copolymer; Step D2, adding the antibacterial copolymer prepared in step D1 to 2,2,2-trifluoroethanol, and mixing them uniformly to form a dispersion A; adding hyperbranched polylysine to deionized water, and mixing them uniformly to form a dispersion B; mixing the dispersion A and the dispersion B to obtain a spinning solution; Step D3, electrospinning the spinning solution to obtain raw fibers; Step D4: dispersing sodium alginate in water to form a modifier, spraying the modifier on the surface of the original fiber, and then post-processing to obtain antibacterial nanofibers.

2. The medical antibacterial dressing according to claim 1, characterized in that: The mass ratio of acrylamide β-cyclodextrin quaternary ammonium salt, 4-methyl-5-vinylthiazole, N-trihydroxymethyl methacrylamide, initiator and high boiling point solvent in step D1 is 1:(0.8-1.2):(3-5):(0.05-0.08):(20-35).

3. The medical antibacterial dressing according to claim 1, characterized in that: The initiator is azobisisobutyronitrile; the high boiling point solvent is at least one of dimethyl sulfoxide, N,N-dimethylformamide, and N-methylpyrrolidone; and the inert gas is any one of nitrogen, helium, neon, and argon.

4. The medical antibacterial dressing according to claim 1, characterized in that: The mass ratio of the antibacterial copolymer and 2,2,2-trifluoroethanol in step D2 is (1.2-2.2):

10.

5. The medical antibacterial dressing according to claim 1, characterized in that: The mass ratio of the hyperbranched polylysine to deionized water in step D2 is (1-3):

10.

6. The medical antibacterial dressing according to claim 1, characterized in that: The mass ratio of dispersion A to dispersion B in step D2 is 10:(0.4-1.6).

7. The medical antibacterial dressing according to claim 1, characterized in that: The process parameters of the electrospinning described in step D3 include: a voltage of 15-35 kV, a distance between the spinneret and the receiver of 12-30 cm; the mass ratio of sodium alginate to water described in step D4 is 1:(4-6); the mass ratio of sodium alginate to fibril is (1-2):10; the post-treatment described in step D4 is specifically: vacuum drying for 5-10 hours under vacuum gauge pressure of -0.05 to -0.15 MPa and temperature of 80-90°C.

8. The medical antibacterial dressing according to claim 1, characterized in that: The rose fiber is Rose fiber viscose 32S; the fiber fineness of the bamboo fiber is 6D.

9. A method for preparing the medical antibacterial dressing according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: chitosan composite fiber, rose fiber, bamboo fiber and antibacterial nanofiber are blended to form dressing cloth, which is then rolled by a rolling mill and cut into 5×5 cm squares, and the medical antibacterial dressing is obtained after packaging.

10. The method for preparing the medical antibacterial dressing according to claim 9, characterized in that: The temperature of the rolling mill during rolling is 115° C.-155° C., and the speed at which the grey cloth passes through the hot roller of the rolling mill is 130-320 rpm.

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