High-salt-resistant organic fertilizer fermentation inoculant screened from mangrove forest mud flat

Abstract

The invention discloses a high-salt-resistant organic fertilizer fermentation inoculant screened from mangrove forest mud flat. Comprising a main fermentation inoculant and an auxiliary fermentation inoculant, the use ratio of the main fermentation inoculant to the auxiliary fermentation inoculant is 5: 1, the main fermentation inoculant comprises Acidothermophilus, mangrove endophytic fungi, Bacillus subtilis, Bacillus licheniformis, Trichoderma viride and Aspergillus niger, and the auxiliary fermentation inoculant comprises Lactobacillus plantarum, Lactobacillus acidophilus and photosynthetic bacteria. The high-salt-resistant organic fertilizer fermentation inoculant screened from the mangrove forest mud flat can accelerate the fermentation and decomposition speed of organic matters in pig manure, reduce the turning frequency, shorten the fermentation and decomposition time and improve the practicability.

Description

Technical Field

[0001] The invention relates to the technical field of organic fertilizer fermentation, in particular to a high-salt-resistant organic fertilizer fermentation bacterial agent screened from mangrove tidal flats. Background Art

[0002] High-salt tolerant pig manure organic fertilizer strains, namely biological organic fertilizer strains, are strains used to process pig manure into organic fertilizer after fermentation, deodorization and complete decomposition. High-salt tolerant pig manure organic fertilizer fermentation agent, namely organic material decomposition agent, can decompose protein, cellulose, hemicellulose, lignin, etc. High-salt tolerant pig manure organic fertilizer fermentation agent has a high content of effective live bacteria, strong degradation ability, and can play a role in high-salinity environment. At the same time, it can achieve the effects of warming, deodorization, elimination of pests and diseases and improvement of nutrients. Under high-salinity conditions, it can quickly decompose and mineralize carbon, nitrogen, phosphorus, potassium, sulfur, etc. in the pile to form simple organic matter, which can be further decomposed into nutrients that can be absorbed by crops. It is mainly used for pig breeding and pig slaughtering waste treatment, and can be quickly turned into organic fertilizer. When the existing pig manure organic fertilizer fermentation agent is used, the fermentation effect of the pig manure is poor, the survival rate of the bacteria is low, and it takes a long time to fully decompose the pig manure. Due to the long time, more times of turning the pile are required, which leads to slow decomposition of the pig manure and poor practicality.

[0003] As a huge ecosystem, mangroves have strong recycling capabilities. The salt-tolerant bacteria in their mudflats can efficiently decompose biological debris, so they have strong salt tolerance and degradation capabilities. Summary of the invention

[0004] The purpose of the present invention is to provide a high-salt resistant pig manure organic fertilizer fermentation bacterial agent which can improve the survival rate of bacteria, accelerate the fermentation and decomposition speed of pig manure, reduce the number of compost turning times, shorten the fermentation and decomposition time, and improve practicality.

[0005] To achieve the above-mentioned purpose, the present invention provides a high-salt-tolerant pig manure organic fertilizer fermentation agent screened from mangrove tidal flats, characterized in that it comprises a main fermentation agent and an auxiliary fermentation agent, and the use ratio of the main fermentation agent and the auxiliary fermentation agent is 5:1, wherein the main fermentation agent comprises Acidothermus cellulolyticus, mangrove endophytic fungi, Bacillus subtilis, Bacillus licheniformis, Trichoderma viride and Aspergillus niger, and the auxiliary fermentation agent comprises Lactobacillus plantarum, Lactobacillus acidophilus and photosynthetic bacteria.

[0006] Furthermore, the high-salt-tolerant pig manure organic fertilizer fermentation agent selected from mangrove mudflats also includes bran powder and protease, the total amount of the bran powder and protease added is 5 to 10 times the total weight of the main fermentation agent and the auxiliary fermentation agent, and the weight ratio of the bran powder and the protease is 10 to 12:1.

[0007] Furthermore, the addition ratio of each component in the main fermentation agent is:

[0008] Acidothermus cellulolyticus 1 to 2 parts;

[0009] 1-2 copies of mangrove endophytic fungi;

[0010] Bacillus subtilis 0.8-1.1 parts;

[0011] 0.5-0.7 parts of Bacillus licheniformis;

[0012] 0.3-0.5 parts of viride;

[0013] 0.2-0.4 parts of Aspergillus niger.

[0014] Furthermore, the addition ratio of each component in the auxiliary fermentation agent is:

[0015] 1-1.8 parts of Lactobacillus plantarum;

[0016] Lactobacillus acidophilus 1-1.6 parts;

[0017] 0.6 to 1 part of photosynthetic bacteria.

[0018] The present invention also provides a method for preparing the high-salt-tolerant pig manure organic fertilizer fermentation agent selected from mangrove tidal flats, which is characterized by comprising the following steps:

[0019] S1. Test tube slant culture of strains: strains stored in sand tubes or freeze-drying tanks of different strains are inoculated on different test tube slant surfaces in a sterile manner for test tube slant culture;

[0020] S2. Shake flask culture of strains: The mature strains cultured on the test tube slope are inoculated again into the shake flask medium in a sterile manner for further culture;

[0021] S3, seed tank expansion culture: the strains cultured in shake flasks are transferred to seed tanks for expansion culture;

[0022] S4, fermentation tank culture: the bacteria after expanded culture are transferred to the fermentation tank for fermentation culture to be qualified;

[0023] S5. Preparation of bacterial agents: preparing bacterial liquids from the bacterial species cultured step by step, and preparing fermentation bacterial agents according to the proportions;

[0024] S6, bacterial liquid drying: drying the prepared fermentation bacterial agent by vacuum freeze drying to obtain a dry high-salt-tolerant pig manure organic fertilizer fermentation bacterial agent selected from mangrove tidal flats;

[0025] S7, live bacteria detection: the dried high-salt-tolerant pig manure organic fertilizer fermentation agent selected from the mangrove beach is tested for live bacteria, and the qualified agents are packaged;

[0026] S8. Repackaging: The high-salt-tolerant pig manure organic fertilizer fermentation agent screened from the mangrove mudflats after testing is mixed with bran powder and protease in proportion and then repacked for storage and transportation.

[0027] Furthermore, the pH of the culture medium in the seed tank is 7.2-7.8, and the culture temperature is 28-32°C.

[0028] Furthermore, in the later stage of the expanded culture, sterile air is introduced into the seed tank, and stirring is performed to allow the bacteria in the seed tank to adapt to the fermentation environment in the fermentation tank.

[0029] Furthermore, when testing live bacteria, the number of live bacteria is ≥ 9.5 billion / gram, which is qualified.

[0030] The present invention also protects the use of the high-salt-tolerant pig manure organic fertilizer fermentation bacterial agent selected from mangrove tidal flats for pig manure fermentation.

[0031] Furthermore, 0.05-0.1% of the main fermentation agent and the auxiliary fermentation agent and 0.25-1% of the fermentation agent mixture with bran powder and protease of the pig manure to be fermented are weighed, mixed and stirred evenly with the pig manure, the moisture content is adjusted to 40%-50%, and composting fermentation and decomposition are started. The pile temperature rises rapidly to 50-60°C within 2 days, and the fermentation and decomposition can be completed in 5-7 days, and the pile is turned over every 1-2 days.

[0032] Compared with the prior art, the invention has the following beneficial effects: the number of live bacteria in the organic fermentation bacterial fertilizer is high, so in the process of fermenting and maturing pig manure, the maturation speed can be accelerated, 0.05-0.1% of the mass of the pig manure to be fermented of the main fermentation bacterial agent and the auxiliary fermentation bacterial agent of the invention, and 0.25-1% of the mass of the fermentation bacterial agent mixture with bran powder and protease are weighed, mixed and stirred evenly with the pig manure, the moisture content is adjusted to 40%-50%, and composting fermentation and maturation are started;

[0033] When the organic fertilizer fermentation agent of the present invention is used for organic fertilizer fermentation and cultivation, the mixture of pig manure and the agent heats up quickly, the pile temperature rises rapidly to 50-60° C. within 2 days, and the fermentation and decomposition can be completed in 5-7 days. The pile is turned over every 1-2 days. High temperature is generated during the fermentation process, which kills harmful bacteria and insect eggs in the pig manure and completely decomposes the pig manure. When the odor gradually dissipates, the material no longer heats up and the shape becomes loose and turns dark brown, the fermentation is completed.

[0034] High temperature is generated during the pig manure fermentation process, which can kill harmful bacteria and insect eggs in the pig manure, and completely decompose the pig manure to fully release the nutrients therein, so that the nutrients can be fully utilized and the fertilizer effect is better. The organic fertilizer fermented by the present invention can effectively improve the compaction of the soil and inhibit the occurrence of diseases and insect pests in the soil;

[0035] The organic fertilizer fermentation agent is easy to operate and has a wide range of uses, and can be widely applied to various poultry and livestock manure.

[0036] Therefore, when the organic fertilizer fermentation agent of the present invention is used to ferment pig manure, the fermentation speed of various organic waste gases can be accelerated, the number of compost turning times can be reduced, the fermentation time can be shortened, and the practicality can be improved. DETAILED DESCRIPTION

[0037] Embodiments of the present invention are described in detail below, and the examples of the embodiments are intended to be used to explain the present invention, and should not be construed as limiting the present invention. In the embodiments, if specific techniques or conditions are not indicated, the techniques or conditions described in the literature in this area or the product specifications are carried out. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products that can be obtained commercially. In the following embodiments, if not clearly stated, "%" refers to weight percentage.

[0038] Example 1: Preparation of high-salt-tolerant pig manure organic fertilizer fermentation agent screened from mangrove tidal flats

[0039] The organic fertilizer fermentation agent of this embodiment includes a main fermentation agent and an auxiliary fermentation agent, and the weight ratio of the main fermentation agent and the auxiliary fermentation agent is 5:1, wherein the main fermentation agent includes Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Trichoderma viride and Aspergillus niger, and the auxiliary fermentation agent includes Lactobacillus plantarum, Lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.

[0040] The organic fertilizer fermentation agent also includes bran powder and protease, the total weight of the bran powder and protease added is 5 times the total weight of the main fermentation agent and the auxiliary fermentation agent, and the weight ratio of the bran powder and the protease is 10:1.

[0041] Among them, the added weight ratio of each component in the main fermentation agent is:

[0042] Acidothermus cellulolyticus 2 parts;

[0043] 1 copy of mangrove endophytic fungi;

[0044] 1 part of Bacillus subtilis;

[0045] 0.5 parts of Bacillus licheniformis;

[0046] 0.3 parts of viride;

[0047] 0.2 part of Aspergillus niger.

[0048] Among them, the added weight ratio of each component in the auxiliary fermentation agent is:

[0049] 1 portion of Lactobacillus plantarum;

[0050] 1 portion of Lactobacillus acidophilus;

[0051] 0.6 parts of photosynthetic bacteria.

[0052] The preparation method comprises the following steps:

[0053] S1. Test tube slant culture of strains: strains stored in sand tubes or freeze-drying tanks of different strains are inoculated on different test tube slant surfaces in a sterile manner for test tube slant culture;

[0054] S2. Shake flask culture of strains: The mature strains cultured on the test tube slope are inoculated again into the shake flask medium in a sterile manner for further culture;

[0055] S3, seed tank expansion culture: the strains after shake flask culture are transferred to the seed tank, and the expansion culture is carried out in the seed tank. The pH of the culture medium in the seed tank is 7.4, and the culture temperature is 32°C. In the later stage of expansion culture, sterile air is introduced into the seed tank, and stirring is performed to make the strains in the seed tank adapt to the fermentation environment in the fermentation tank;

[0056] S4, fermentation tank culture: transfer the expanded cultured bacteria to the fermentation tank for fermentation culture. During the live bacteria test, the live bacteria count ≥ 9.5 billion / g is qualified;

[0057] S5, preparation of bacterial agent: preparing bacterial liquid by gradually diffusing and cultivating bacterial species, and preparing fermentation bacterial agent according to the proportion;

[0058] S6, bacterial liquid drying: drying the prepared fermentation bacterial agent by vacuum freeze drying to obtain a dry organic fertilizer fermentation bacterial agent;

[0059] S7, live bacteria detection: the dried organic fertilizer fermentation agent is tested for live bacteria, and the qualified agents are packaged;

[0060] S8. Repackaging: Mix the tested organic fertilizer fermentation agent with bran powder and protease in proportion and then repack it for storage and transportation.

[0061] Weigh 0.05-0.1% of the main fermentation agent and the auxiliary fermentation agent of the pig manure mass to be fermented, that is, 0.25-1% of the fermentation agent mixture with bran powder and protease, mix and stir evenly with the pig manure, adjust the moisture content to 40%-50%, start composting and fermentation, the pile temperature will rise rapidly to 50-60°C within 2 days, and the fermentation and maturity can be completed in 5-7 days. Turn the pile every 1-2 days. High temperature is generated during the fermentation process to kill harmful bacteria and insect eggs in the pig manure and completely decompose the pig manure.

[0062] Example 2: Preparation of high-salt-tolerant pig manure organic fertilizer fermentation agent screened from mangrove tidal flats

[0063] The organic fertilizer fermentation agent of this embodiment includes a main fermentation agent and an auxiliary fermentation agent, and the usage ratio of the main fermentation agent and the auxiliary fermentation agent is 6:1, wherein the main fermentation agent includes Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Trichoderma viride and Aspergillus niger, and the auxiliary fermentation agent includes Lactobacillus plantarum, Lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.

[0064] The organic fertilizer fermentation agent also includes bran powder and protease, the total amount of the bran powder and protease added is 7 times the total amount of the main fermentation agent and the auxiliary fermentation agent added, and the weight ratio of the bran powder and the protease is 12:1.

[0065] Among them, the added weight ratio of each component in the main fermentation agent is:

[0066] Acidothermus cellulolyticus 1.5 parts;

[0067] 1.5 copies of endophytic fungi in mangroves;

[0068] Bacillus subtilis 0.9 parts;

[0069] 0.6 parts of Bacillus licheniformis;

[0070] 0.3 parts of viride;

[0071] 0.2 part of Aspergillus niger.

[0072] Among them, the added weight ratio of each component in the auxiliary fermentation agent is:

[0073] 1.3 parts of Lactobacillus plantarum;

[0074] Lactobacillus acidophilus 1.3 parts;

[0075] 0.7 parts of photosynthetic bacteria.

[0076] The preparation method comprises the following steps:

[0077] S1. Test tube slant culture of strains: strains stored in sand tubes or freeze-drying tanks of different strains are inoculated on different test tube slant surfaces in a sterile manner for test tube slant culture;

[0078] S2. Shake flask culture of strains: The mature strains cultured on the test tube slope are inoculated again into the shake flask medium in a sterile manner for further culture;

[0079] S3, seed tank expansion culture: the strains after shake flask culture are transferred to the seed tank, and the expansion culture is carried out in the seed tank. The pH of the culture medium in the seed tank is 7.6, and the culture temperature is 28°C. In the later stage of expansion culture, sterile air is introduced into the seed tank, and stirring is performed to make the strains in the seed tank adapt to the fermentation environment in the fermentation tank;

[0080] S4, fermentation tank culture: transfer the expanded cultured bacteria to the fermentation tank for fermentation culture. During the live bacteria test, the live bacteria count ≥ 9.5 billion / g is qualified;

[0081] S5, preparation of bacterial agent: preparing bacterial liquid by gradually diffusing and cultivating bacterial species, and preparing fermentation bacterial agent according to the proportion;

[0082] S6, bacterial liquid drying: drying the prepared fermentation bacterial agent by vacuum freeze drying to obtain a dry organic fertilizer fermentation bacterial agent;

[0083] S7, live bacteria detection: the dried organic fertilizer fermentation agent is tested for live bacteria, and the qualified agents are packaged;

[0084] S8. Repackaging: Mix the tested organic fertilizer fermentation agent with bran powder and protease in proportion and then repack it for storage and transportation.

[0085] Weigh 0.05-0.1% of the main fermentation agent and the auxiliary fermentation agent of the pig manure mass to be fermented, that is, 0.25-1% of the fermentation agent mixture with bran powder and protease, mix and stir evenly with the pig manure, adjust the moisture content to 40%-50%, start composting and fermentation, the pile temperature will rise rapidly to 50-60°C within 2 days, and the fermentation and maturity can be completed in 5-7 days. Turn the pile every 1-2 days. High temperature is generated during the fermentation process to kill harmful bacteria and insect eggs in the pig manure and completely decompose the pig manure.

[0086] Example 3: Preparation of high-salt-tolerant pig manure organic fertilizer fermentation agent screened from mangrove tidal flats

[0087] The organic fertilizer fermentation agent of this embodiment includes a main fermentation agent and an auxiliary fermentation agent, and the weight ratio of the main fermentation agent and the auxiliary fermentation agent is 5:1, wherein the main fermentation agent includes Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Trichoderma viride and Aspergillus niger, and the auxiliary fermentation agent includes Lactobacillus plantarum, Lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.

[0088] The organic fertilizer fermentation agent also includes bran powder and protease, the total amount of the bran powder and protease added is 10 times the total weight of the main fermentation agent and the auxiliary fermentation agent, and the weight ratio of the bran powder and the protease is 11:1.

[0089] Among them, the added weight ratio of each component in the main fermentation agent is:

[0090] Acidothermus cellulolyticus 1.6 parts;

[0091] 1.3 copies of endophytic fungi in mangroves;

[0092] 1.1 parts of Bacillus subtilis;

[0093] 0.6 parts of Bacillus licheniformis;

[0094] 0.3 parts of viride;

[0095] 0.2 part of Aspergillus niger.

[0096] Among them, the added weight ratio of each component in the auxiliary fermentation agent is:

[0097] 1.5 parts of Lactobacillus plantarum;

[0098] 1.5 parts of Lactobacillus acidophilus;

[0099] 0.7 parts of photosynthetic bacteria.

[0100] A method for culturing an organic fertilizer fermentation agent comprises the following steps:

[0101] S1. Test tube slant culture of strains: strains stored in sand tubes or freeze-drying tanks of different strains are inoculated on different test tube slant surfaces in a sterile manner for test tube slant culture;

[0102] S2. Shake flask culture of strains: The mature strains cultured on the test tube slope are inoculated again into the shake flask medium in a sterile manner for further culture;

[0103] S3, seed tank expansion culture: the strains after shake flask culture are transferred to the seed tank, and the expansion culture is carried out in the seed tank. The pH of the culture medium in the seed tank is 7.8, and the culture temperature is 30°C. In the later stage of expansion culture, sterile air is introduced into the seed tank, and stirring is performed to make the strains in the seed tank adapt to the fermentation environment in the fermenter;

[0104] S4, fermentation tank culture: transfer the expanded cultured bacteria to the fermentation tank for fermentation culture. During the live bacteria test, the live bacteria count ≥ 9.5 billion / g is qualified;

[0105] S5, preparation of bacterial agent: preparing bacterial liquid by gradually diffusing and cultivating bacterial species, and preparing fermentation bacterial agent according to the proportion;

[0106] S6, bacterial liquid drying: drying the prepared fermentation bacterial agent by vacuum freeze drying to obtain a dry organic fertilizer fermentation bacterial agent;

[0107] S7, live bacteria detection: the dried organic fertilizer fermentation agent is tested for live bacteria, and the qualified agents are packaged;

[0108] S8. Repackaging: Mix the tested organic fertilizer fermentation agent with bran powder and protease in proportion and then repack it for storage and transportation.

[0109] Weigh 0.05-0.1% of the main fermentation agent and the auxiliary fermentation agent of the pig manure mass to be fermented, that is, 0.25-1% of the fermentation agent mixture with bran powder and protease, mix and stir evenly with the pig manure, adjust the moisture content to 40%-50%, start composting and fermentation, the pile temperature will rise rapidly to 50-60°C within 2 days, and the fermentation and maturity can be completed in 5-7 days. Turn the pile every 1-2 days. High temperature is generated during the fermentation process to kill harmful bacteria and insect eggs in the pig manure and completely decompose the pig manure.

[0110] Example 4: Preparation of high-salt-tolerant pig manure organic fertilizer fermentation agent screened from mangrove tidal flats

[0111] The organic fertilizer fermentation agent of this embodiment includes a main fermentation agent and an auxiliary fermentation agent, and the weight ratio of the main fermentation agent and the auxiliary fermentation agent is 5.5:1, wherein the main fermentation agent includes Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Trichoderma viride and Aspergillus niger, and the auxiliary fermentation agent includes Lactobacillus plantarum, Lactobacillus acidophilus, actinomycetes and photosynthetic bacteria.

[0112] The organic fertilizer fermentation agent also includes bran powder and protease, the total amount of the bran powder and protease added is 8 times the total weight of the main fermentation agent and the auxiliary fermentation agent, and the weight ratio of the bran powder and the protease is 11.5:1.

[0113] Among them, the added weight ratio of each component in the main fermentation agent is:

[0114] Acidothermus cellulolyticus 1.6 parts;

[0115] 1.3 copies of endophytic fungi in mangroves;

[0116] 1.1 parts of Bacillus subtilis;

[0117] 0.6 parts of Bacillus licheniformis;

[0118] 0.3 parts of viride;

[0119] 0.2 part of Aspergillus niger.

[0120] Among them, the added weight ratio of each component in the auxiliary fermentation agent is:

[0121] 1.5 parts of Lactobacillus plantarum;

[0122] 1.5 parts of Lactobacillus acidophilus;

[0123] 0.7 parts of photosynthetic bacteria.

[0124] The preparation method comprises the following steps:

[0125] S1. Test tube slant culture of strains: strains stored in sand tubes or freeze-drying tanks of different strains are inoculated on different test tube slant surfaces in a sterile manner for test tube slant culture;

[0126] S2. Shake flask culture of strains: The mature strains cultured on the test tube slope are inoculated again into the shake flask medium in a sterile manner for further culture;

[0127] S3, seed tank expansion culture: the strains after shake flask culture are transferred to the seed tank, and the expansion culture is carried out in the seed tank. The pH of the culture medium in the seed tank is 7.0, and the culture temperature is 29° C. In the later stage of expansion culture, sterile air is introduced into the seed tank, and stirring is performed to make the strains in the seed tank adapt to the fermentation environment in the fermentation tank;

[0128] S4, fermentation tank culture: transfer the expanded cultured bacteria to the fermentation tank for fermentation culture. During the live bacteria test, the live bacteria count ≥ 9.5 billion / g is qualified;

[0129] S5, preparation of bacterial agent: preparing bacterial liquid by gradually diffusing and cultivating bacterial species, and preparing fermentation bacterial agent according to the proportion;

[0130] S6, bacterial liquid drying: drying the prepared fermentation bacterial agent by vacuum freeze drying to obtain a dry organic fertilizer fermentation bacterial agent;

[0131] S7, live bacteria detection: the dried organic fertilizer fermentation agent is tested for live bacteria, and the qualified agents are packaged;

[0132] S8. Repackaging: Mix the tested organic fertilizer fermentation agent with bran powder and protease in proportion and then repack it for storage and transportation.

[0133] Weigh 0.05-0.1% of the main fermentation agent and the auxiliary fermentation agent of the pig manure mass to be fermented, that is, 0.25-1% of the fermentation agent mixture with bran powder and protease, mix and stir evenly with the pig manure, adjust the moisture content to 40%-50%, start composting and fermentation, the pile temperature will rise rapidly to 50-60°C within 2 days, and the fermentation and maturity can be completed in 5-7 days. Turn the pile every 1-2 days. High temperature is generated during the fermentation process to kill harmful bacteria and insect eggs in the pig manure and completely decompose the pig manure.

[0134] The organic fermentation bacterial fertilizer of the present invention has a high number of live bacteria, so in the process of fermenting and maturing pig manure, the maturation speed can be accelerated, 0.05-0.1% of the mass of the agricultural pig manure to be fermented by the main fermentation bacterial agent and the auxiliary fermentation bacterial agent of the present invention, that is, 0.25-1% of the mass of the pig manure by weight of the fermentation bacterial agent mixture with bran powder and protease is weighed, mixed and stirred evenly with the pig manure, and the moisture content is adjusted to 40%-50%, and composting fermentation and maturation are started;

[0135] When the organic fertilizer fermentation agent of the present invention is used for organic fertilizer fermentation and cultivation, the mixture of pig manure and the agent heats up quickly, the pile temperature rises rapidly to 50-60° C. within 2 days, and the fermentation and decomposition can be completed in 5-7 days. The pile is turned over every 1-2 days. When the odor gradually dissipates, the material no longer heats up and the shape becomes loose and turns dark brown, the fermentation is completed.

[0136] High temperature is generated during the pig manure fermentation process, which can kill harmful bacteria and insect eggs in the pig manure, and completely decompose the pig manure to fully release the nutrients therein, so that the nutrients can be fully utilized and the fertilizer effect is better. The organic fertilizer fermented by the present invention can effectively improve the compaction of the soil and inhibit the occurrence of diseases and insect pests in the soil;

[0137] The organic fertilizer fermentation agent is easy to operate and has a wide range of uses. It can be widely used in various crop straws, livestock manure, domestic garbage, agricultural product processing waste and other pig manure;

[0138] Therefore, when the organic fertilizer fermentation agent of the present invention is used to ferment pig manure, the fermentation speed of various organic waste gases can be accelerated, the number of compost turning times can be reduced, the fermentation time can be shortened, and the practicality can be improved.

[0139] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention without departing from the principles and intent of the present invention.

Claims

1. A high-salt-tolerant organic fertilizer fermentation agent selected from mangrove tidal flats, characterized in that: The invention comprises a main fermentation agent and an auxiliary fermentation agent, wherein the main fermentation agent and the auxiliary fermentation agent are used in a ratio of 5:1, wherein the main fermentation agent comprises Acidothermus cellulolyticus, mangrove endophytic fungi, Bacillus subtilis, Bacillus licheniformis, Trichoderma viride and Aspergillus niger, and the auxiliary fermentation agent comprises Lactobacillus plantarum, Lactobacillus acidophilus and photosynthetic bacteria.

2. The high-salt-tolerant organic fertilizer fermentation agent selected from mangrove tidal flats as claimed in claim 1, characterized in that: The high-salt-tolerant pig manure organic fertilizer fermentation agent selected from mangrove mudflats also includes bran powder and protease, the total amount of the bran powder and protease added is 5 to 10 times the total weight of the main fermentation agent and the auxiliary fermentation agent, and the weight ratio of the bran powder and the protease is 10 to 12:

1.

3. The high-salt-tolerant organic fertilizer fermentation agent selected from mangrove tidal flats as claimed in claim 1, characterized in that: The addition ratio of each component in the main fermentation agent is: Acidothermus cellulolyticus 1 to 2 parts; 1-2 copies of mangrove endophytic fungi; Bacillus subtilis 0.8-1.1 parts; 0.5-0.7 parts of Bacillus licheniformis; 0.3-0.5 parts of viride; 0.2-0.4 parts of Aspergillus niger.

4. The high-salt-tolerant organic fertilizer fermentation agent selected from mangrove tidal flats as claimed in claim 1, characterized in that: The addition ratio of each component in the auxiliary fermentation bacterial agent is: 1-1.8 parts of Lactobacillus plantarum; Lactobacillus acidophilus 1-1.6 parts; 0.6 to 1 part of photosynthetic bacteria.

5. A method for preparing a high-salt-tolerant organic fertilizer fermentation agent selected from mangrove tidal flats as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: S1. Test tube slant culture of strains: strains stored in sand tubes or freeze-drying tanks of different strains are inoculated on different test tube slant surfaces in a sterile manner for test tube slant culture; S2. Shake flask culture of strains: The mature strains cultured on the test tube slope are inoculated again into the shake flask medium in a sterile manner for further culture; S3, seed tank expansion culture: the strains cultured in shake flasks are transferred to seed tanks for expansion culture; S4, fermentation tank culture: the bacteria after expanded culture are transferred to the fermentation tank for fermentation culture to be qualified; S5. Preparation of bacterial agents: preparing bacterial liquids from the bacterial species cultured step by step, and preparing fermentation bacterial agents according to the proportions; S6, bacterial liquid drying: drying the prepared fermentation bacterial agent by vacuum freeze drying to obtain a dry high-salt-tolerant pig manure organic fertilizer fermentation bacterial agent selected from mangrove tidal flats; S7, live bacteria detection: the dried high-salt-tolerant pig manure organic fertilizer fermentation agent selected from the mangrove beach is tested for live bacteria, and the qualified agents are packaged; S8. Repackaging: The high-salt-tolerant pig manure organic fertilizer fermentation agent screened from the mangrove mudflats after testing is mixed with bran powder and protease in proportion and then repacked for storage and transportation.

6. The method for preparing the high-salt-tolerant pig manure organic fertilizer fermentation agent selected from mangrove tidal flats as claimed in claim 5, characterized in that: The pH of the culture medium in the seed tank is 7.2-7.8, and the culture temperature is 28-32°C.

7. The method for preparing the high-salt-tolerant pig manure organic fertilizer fermentation agent selected from mangrove tidal flats as claimed in claim 5, characterized in that: In the later stage of the expanded culture, sterile air is introduced into the seed tank, and stirring is performed to allow the bacteria in the seed tank to adapt to the fermentation environment in the fermentation tank.

8. The method for preparing the high-salt-tolerant pig manure organic fertilizer fermentation agent selected from mangrove tidal flats as claimed in claim 5, characterized in that: When testing live bacteria, the number of live bacteria ≥ 9.5 billion / gram is qualified.

9. Use of the high-salt-tolerant organic fertilizer fermentation agent selected from mangrove tidal flats as described in any one of claims 1 to 4 for pig manure fermentation.

10. The use according to claim 9, characterized in that Weigh 0.05-0.1% of the main fermentation agent and the auxiliary fermentation agent of the pig manure mass to be fermented, and 0.25-1% of the fermentation agent mixture with bran powder and protease, mix them with the pig manure and stir them evenly, adjust the moisture content to 40%-50%, start composting and fermentation, the pile temperature will rise rapidly to 50-60°C within 2 days, turn the pile every 1-2 days, and the fermentation and maturity can be completed in 5-7 days.