Microreactor for enhancing hydrogen production through symbiosis of phycomycetes and preparation method of microreactor
Through algae symbiosis, the hydrogen-producing microreactor is strengthened, and the microalgae symbiotic structure of electroactive bacteria and hydrogen-producing microalgae is solved by using electroactive bacteria and hydrogen-producing microalgae symbiotic structure, combining the printing technology of sodium alginate and calcium chloride and soaking with chitosan solution. The problem of low hydrogen production yield has achieved efficient hydrogen production and long-term stability.
Patent Information
- Application Number
- CN202411342381.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-25
- Publication Date
- 2024-12-31
AI Technical Summary
Microalgae photobiological hydrogen production is low and has a limited duration. Due to oxygen inhibition, it is difficult to increase hydrogen production.
Algae symbiosis enhances the hydrogen production microreactor, by using electroactive bacteria as the outer layer and hydrogen-producing microalgae as the inner layer, the sphere core is printed using sodium alginate and calcium chloride solution, and in the bacteria-chitosan solution Soak in the middle to form a conical serrated structure with uniformly distributed surface of the sphere, increasing the contact area and stability.
Long-term anaerobic microenvironment and high hydrogen enzyme activity were achieved, significantly improving the yield and sustainability of hydrogen, and enhancing hydrogen productivity and material conversion.
Smart Images
Figure CN119220532A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of environmental energy, and specifically relates to an algae-bacteria symbiotic enhanced hydrogen production microreactor and a preparation method thereof. Background Art
[0002] Excessive consumption of fossil fuels has caused serious environmental problems, accelerated global warming and aggravated climate change, and affected the social and economic development of human society. The development of green, clean and renewable energy is an effective way to solve the above problems. Biofuels such as bioethanol, biodiesel, and biohydrogen are considered to be substitutes for fossil fuels. Their use can significantly reduce carbon dioxide emissions and is expected to alleviate or even solve global energy and ecological environmental problems.
[0003] Among biofuels, biohydrogen is considered to be one of the most promising alternative energy sources. Its combustion produces more heat than hydrocarbons and does not produce carbon dioxide after combustion, which is very environmentally friendly. As early as the 20th century, renewable hydrogen and biohydrogen produced by solar energy have attracted people's attention. Microalgae can quickly produce hydrogen in a short period of time after the transition from dark to light environment. However, the oxygen produced by photosynthesis strongly and irreversibly inhibits the activity of hydrogenase, resulting in a short duration of microalgae photobiological hydrogen production and difficulty in increasing hydrogen production. Based on the proposal of enhanced measures such as respiratory enhancement, nutrient deprivation and genetic engineering of oxygen-resistant hydrogenase, the negative impact of oxygen on biohydrogen production has been reduced to a certain extent, and the efficiency of microalgae hydrogen production has been promoted.
[0004] Although the technical means to enhance hydrogen production from microalgae have been developed for many years, the productivity of hydrogen production from microalgae is relatively low and the production time is limited, which is still the main challenge and bottleneck for the future development of microalgae photobiological hydrogen production. Summary of the invention
[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide an algae-bacteria symbiotic enhanced hydrogen production microreactor and a preparation method thereof. The preparation method of the present invention is simple and has high repeatability. The prepared hydrogen production microreactor can maintain a long-term anaerobic microenvironment and high hydrogenase activity, thereby effectively increasing the hydrogen production of microalgae.
[0006] The technical solution of the present invention is achieved in this way:
[0007] A method for preparing an algae-bacteria symbiotic enhanced hydrogen production microreactor specifically comprises the following steps:
[0008] (1) mixing the hydrogen-producing microalgae and the sodium alginate solution to obtain a microalgae-sodium alginate solution, and then filling the solution into one syringe of a dual-channel microfluidic pump, and filling the calcium chloride solution into another syringe of the dual-channel microfluidic pump;
[0009] (2) connecting the dual-channel microfluidic pump to the print head of the 3D printer, and pumping out the microalgae-sodium alginate solution and the calcium chloride solution from the print head according to the preset hydrogen-producing microalgae core model, thereby printing the hydrogen-producing microalgae core of the hydrogen-producing microreactor;
[0010] (3) The electroactive bacteria and the chitosan solution are mixed to obtain a bacteria-chitosan solution, and the hydrogen-producing microalgae core of step (2) is immersed in the bacteria-chitosan solution and stirred for 25 to 30 minutes to obtain the hydrogen-producing microreactor.
[0011] Furthermore, the hydrogen-producing microalgae is one of Chlamydomonas, Synechocystis, Scenedesmus and Chlorella.
[0012] Furthermore, in step (1), the volume ratio of the hydrogen-producing microalgae to the sodium alginate solution is 2.5 to 7.5:1.
[0013] Furthermore, the density of the hydrogen-producing microalgae is 5.0-15.0 mg chlorophyll / L; the concentration of the sodium alginate solution is 10-20% (w / v); and the concentration of the calcium chloride solution is 2.0-4.0% (w / v).
[0014] Furthermore, the preset hydrogen-producing microalgae kernel model includes a sphere and a plurality of cone teeth evenly distributed on the surface of the sphere, the diameter of the sphere is 200-800 μm, and the spherical surface distance between two adjacent cone teeth is 60-65 μm.
[0015] Furthermore, the diameter of the sphere is 600 μm, the bottom diameter of the cone teeth is 150 μm, the height of the cone teeth is 100 μm, the cone angle of the cone teeth is 74°, and the center angle of two adjacent cone teeth is 12°.
[0016] Furthermore, in step (2), during printing, the injection flow rate of the microalgae-sodium alginate solution channel is 28-30 μL / s, the injection flow rate of the calcium chloride solution channel is 8-12 μL / s, and the print head movement speed is 45-55 μm / s.
[0017] Furthermore, the electroactive bacteria is one of Shewanella, Geobacter and Desulfomonas, and the density of the electroactive bacteria is 1.0 to 5.0×10 5 cells / mL.
[0018] Furthermore, in step (3), the volume ratio of the electroactive bacteria to the chitosan solution is 1:2-10; the chitosan solution has a chitosan concentration of 1.0-2.0% (w / v), and further contains acetic acid, the concentration of which is 0.5-1.0% (v / v).
[0019] Compared with the prior art, the present invention has the following beneficial effects:
[0020] 1. The hydrogen-producing microreactor obtained by the present invention is an algae-bacteria symbiotic microreactor with an outer layer of electroactive bacteria and an inner layer of hydrogen-producing microalgae. The outer layer of electroactive bacteria can effectively consume the oxygen produced by the external and internal hydrogen-producing microalgae, provide an anaerobic microenvironment conducive to hydrogen production and supply additional electrons for the inner layer of hydrogen-producing microalgae, and the inner layer of hydrogen-producing microalgae provides algae-derived organic metabolites for the electroactive bacteria. In this way, the organic combination of the outer layer of electroactive bacteria and the inner layer of hydrogen-producing microalgae can greatly increase the yield and sustainability of hydrogen.
[0021] 2. The inner core of the hydrogen production microreactor of the present invention is a sphere, and a number of conical serrations are evenly distributed on the surface of the sphere, so that the hydrogen production microreactor has a larger specific surface area, and the hydrogen production microreactor has a short material diffusion path, and has the advantages of fast and efficient mass transfer and heat transfer, and therefore has good material conversion rate and hydrogen productivity.
[0022] 3. The spherical surface of the hydrogen-producing microalgae inner core model of the present invention is evenly distributed with cone structures, and the sawtooth structure can greatly increase the contact area between the outer electroactive bacteria and the inner hydrogen-producing microalgae, thereby increasing the electron and organic metabolite transmission capacity; at the same time, the hydrogen-producing microalgae inner core model adopts a cone-sawtooth structure, which can make the outer electroactive bacteria and the inner hydrogen-producing microalgae form an interlocking structure, thereby making the hydrogen-producing microreactor more stable and not easy to decompose, and ultimately it is expected to extend the service life of the hydrogen-producing microreactor. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 -Schematic diagram of the present invention for preparing a hydrogen-producing microalgae reactor.
[0024] Figure 2 -Three views of the hydrogen-producing microalgae kernel model.
[0025] Figure 3 -3D image of the core model of hydrogen-producing microalgae.
[0026] Figure 4 -Enlarged image of the serrated structure on the surface of the core of hydrogen-producing microalgae.
[0027] Figure 5 -3D diagram and front view of hydrogen production microreactor.
[0028] Figure 6 -Microscope observation of hydrogen production microreactor. DETAILED DESCRIPTION
[0029] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0030] The hydrogen-producing microalgae used in the following examples were Chlamydomonas reinhardtii in the logarithmic phase, with a density of 2 mg chlorophyll / L, and the electroactive bacteria were Shewanella spp. grown for 24 h, with a density of 1×106 cells / ml.
[0031] Example 1
[0032] A method for preparing a microreactor for enhanced hydrogen production by algae-bacteria symbiosis, the schematic diagram of which is shown in FIG. Figure 1 As shown, the specific steps include:
[0033] (1) Take the hydrogen-producing microalgae Chlamydomonas reinhardtii in the logarithmic phase, centrifuge at 6000 rpm for 10 min, remove the supernatant, and then mix it with 10 mL of sterilized 10.0% (w / v) sodium alginate solution to obtain a microalgae-sodium alginate solution. Use a 10 mL syringe to draw the microalgae-sodium alginate solution and load it into a dual-channel microfluidic pump. Draw 4% (w / v) calcium chloride solution into another syringe of the dual-channel microfluidic pump;
[0034] (2) Connect the dual-channel microfluidic pump in step (1) to the print head of the 3D printer, print according to the preset hydrogen-producing microalgae core model, pump out the microalgae-sodium alginate solution and the calcium chloride solution from the print head respectively, the injection flow rate of the microalgae-sodium alginate solution channel is 30 μL / s, the injection flow rate of the calcium chloride solution channel is 10 μL / s, and the print head movement speed is 50 μm / s. After printing is completed, let it stand for 30 minutes to ensure complete cross-linking and curing, thereby printing to obtain the hydrogen-producing microalgae core.
[0035] The hydrogen-producing microalgae kernel model is as follows Figure 2 , Figure 3 and Figure 4 As shown, Figure 2 (a), (b) and (c) are the front view, top view and right view respectively. The diameter of the sphere is 600 μm, the base diameter of the cone teeth evenly distributed on its surface is 150 μm, the height is 100 μm, the cone angle is 74°, the spherical center angle of adjacent teeth is 12°, and the spherical distance between adjacent teeth is 60 μm.
[0036] (3) The electroactive bacteria were centrifuged to remove the supernatant and then mixed with 30 mL of a chitosan solution containing 2% (w / v) chitosan and 1% (w / v) acetic acid to obtain a bacteria-chitosan solution. The hydrogen-producing microalgae core in step (2) was immersed in the bacteria-chitosan solution and placed on a magnetic stirrer and stirred at 100 rpm / min for 30 min to form a hydrogen-producing microreactor with a core of hydrogen-producing microalgae and an outer shell of electroactive bacteria.
[0037] According to the above method, in step (1), 15, 25, 50, 75, and 90 mL of hydrogen-producing microalgae, i.e., the density of hydrogen-producing microalgae Chlamydomonas reinhardtii is 3, 5, 10, 15, and 18 mg chlorophyll / L, respectively; in step (3), 1.5, 3, 7.5, 15, and 16.5 mL of electroactive bacteria Shewanella are taken, i.e., the density of electroactive bacteria Shewanella is 0.5, 1, 2.5, 5, and 5.5 × 10 5 cells / mL, an orthogonal experiment was conducted to explore the hydrogen production capacity of the hydrogen production microreactor prepared under different hydrogen-producing microalgae and electroactive bacteria. The schematic diagram of the hydrogen production microreactor is shown in Figure 5 ,in Figure 5 (a) is the 3D image of the hydrogen production microreactor. Figure 5 (b) is a front view of the hydrogen production microreactor. The microscopic observation image of the hydrogen production microreactor obtained when the hydrogen-producing microalgae is 50 mL and the electroactive bacteria Shewanella is 7.5 mL is as shown in the figure. Figure 6 As shown in the figure, it can be seen that the red circle area is the boundary between the inner sphere and the outer shell of the hydrogen-producing microreactor. The light-colored inner layer of the hydrogen-producing microreactor is the hydrogen-producing microalgae inner core (microalgae-sodium alginate), and the dark-colored outer shell is bacteria-chitosan. During the immersion process of the hydrogen-producing microreactor inner core, the bacteria-chitosan solution fills the space between the serrations, and because the color of the bacteria-chitosan is darker than the inner core, the serration structure cannot be observed when displayed.
[0038] The algae-bacteria co-production hydrogen microreactor prepared above was transferred to a 100mL anaerobic bottle containing 50mL TAP-S' medium, sealed and cultured in the dark for two days. TAP-S' is an improvement on TAP (Tris-Acetate-Phosphate) medium. After all sulfur-containing compounds are replaced with chlorine-containing compounds, 1mL / L (v / v) of sodium lactate is further added. The anaerobic bottle was then transferred to a light incubator for light-induced hydrogen production. The culture parameters were: temperature 25°C, light duration 24h, light intensity 100μmol·m -2 ·s -1 The anaerobic bottle was shaken three times a day, 0.5 mL of headspace gas was taken with a 1 mL syringe, and injected into an Agilent gas chromatograph 7890A to detect the headspace hydrogen concentration. Gas chromatography parameter settings: carrier gas was nitrogen, flow rate was 10 mL / min, and the chromatographic column was The molecular sieve filling column has an injection port temperature of 70°C, a column box temperature of 60°C, and a TCD detector temperature of 100°C. The experimental period is 12 days, and the headspace gas is taken every three days to detect the hydrogen concentration. The hydrogen production of each hydrogen production microreactor on the 12th day is shown in Table 1.
[0039] Table 1. Hydrogen production of each hydrogen production microreactor (mL / L)
[0040]
[0041] As can be seen from the table above, when the density of hydrogen-producing microalgae Chlamydomonas reinhardtii is 5-15 mg chlorophyll / L and the density of electroactive bacteria Shewanella is 1-5×10 5 cells / mL, and the hydrogen production was 135.6-160.2 mL / L; and the density of hydrogen-producing microalgae Chlamydomonas reinhardtii was 10 mg chlorophyll / L, and the density of electric-producing bacteria Shewanella was 2.5×10 5 cells / mL, and the maximum hydrogen production was 160.2mL / L.
[0042] Example 2
[0043] A method for preparing an algae-bacteria symbiotic enhanced hydrogen production microreactor specifically comprises the following steps:
[0044] (1) Take 50 mL of hydrogen-producing microalgae Chlamydomonas reinhardtii in the logarithmic phase, centrifuge at 6000 rpm for 10 min, remove the supernatant, and then mix with 10 mL of sterilized 10.0% (w / v) sodium alginate solution to obtain a microalgae-sodium alginate solution. Use a 10 mL syringe to draw the microalgae-sodium alginate solution and load it into a dual-channel microfluidic pump. Draw 4% (w / v) calcium chloride solution into another syringe of the dual-channel microfluidic pump;
[0045] (2) Connect the dual-channel microfluidic pump in step (1) to the print head of the 3D printer, print according to the preset hydrogen-producing microalgae core model, pump out the microalgae-sodium alginate solution and the calcium chloride solution from the print head respectively, the injection flow rate of the microalgae-sodium alginate solution channel is 30 μL / s, the injection flow rate of the calcium chloride solution channel is 10 μL / s, and the print head movement speed is 50 μm / s. After printing is completed, let it stand for 30 minutes to ensure complete cross-linking and curing, thereby printing to obtain the hydrogen-producing microalgae core.
[0046] (3) After centrifuging and removing the supernatant, 7.5 mL of electroactive bacteria was mixed with 30 mL of a chitosan solution containing 2% (w / v) chitosan and 1% (w / v) acetic acid to obtain a bacteria-chitosan solution. The hydrogen-producing microalgae core in step (2) was immersed in the bacteria-chitosan solution and placed on a magnetic stirrer and stirred at 100 rpm / min for 30 min to form a hydrogen-producing microreactor with a core of hydrogen-producing microalgae and an outer shell of electroactive bacteria.
[0047] In this embodiment, a total of six groups were conducted, wherein the control group was a suspension symbiotic culture of hydrogen-producing microalgae and electroactive bacteria, and no fixation or other operations were performed; the other five groups were experimental groups, and the first four groups had spheres with serrated structures on the surface of the core of the core-shell symbiotic microreactor, and the fifth group had a sphere without serrated structures on the surface of the core of the core-shell symbiotic microreactor. The core models of the hydrogen-producing microalgae used in the five groups were:
[0048] (1) 200 μm core-shell symbiotic microreactor: The core model is a spherical structure with a diameter of 200 μm, and its surface is a sawtooth structure. The sawtooth height is 35 μm, and the "teeth" of the sawtooth are cones with a bottom diameter of 50 μm. Furthermore, the cone sawtooth is evenly distributed on the surface of the microalgae core of the microreactor, the adjacent sawtooth spherical center angle is 12°, and the spherical distance between adjacent sawtooth is 60 μm.
[0049] 2) 400μm core-shell symbiotic microreactor: The core model is a spherical structure with a diameter of 400μm, and its surface is a sawtooth structure. The sawtooth height is 66.7μm, and the "teeth" of the sawtooth are cones with a bottom diameter of 100μm. Furthermore, the cone sawtooth is evenly distributed on the surface of the microalgae core of the microreactor, the adjacent sawtooth spherical center angle is 12°, and the spherical distance between adjacent sawtooth is 60μm.
[0050] 3) 600μm core-shell symbiotic microreactor: The core model is a spherical structure with a diameter of 600μm, and its surface is a sawtooth structure. The sawtooth height is 100μm, and the "teeth" of the sawtooth are cones with a bottom diameter of 150μm. Furthermore, the cone sawtooth is evenly distributed on the surface of the microalgae core of the microreactor, the adjacent sawtooth spherical center angle is 12°, and the spherical distance between adjacent sawtooth is 60μm.
[0051] 4) 800μm core-shell symbiotic microreactor: The core model is a spherical structure with a diameter of 800μm, and its surface is a sawtooth structure. The sawtooth height is 133.3μm, and the "teeth" of the sawtooth are cones with a bottom diameter of 200μm. Furthermore, the cone sawtooth is evenly distributed on the surface of the microalgae core of the microreactor, the adjacent sawtooth spherical center angle is 12°, and the spherical distance between adjacent sawtooth is 60μm.
[0052] 5) 600 μm core-shell symbiotic microreactor with smooth core: The core model is a spherical structure with a diameter of 600 μm and a smooth surface, that is, no serrated structure.
[0053] The hydrogen production microreactors prepared in the control group and the above five experimental groups were transferred to 100mL anaerobic bottles containing 50mL TAP-S' medium, sealed and cultured in the dark for two days. TAP-S' is an improvement on TAP (Tris-Acetate-Phosphate) medium. After all sulfur-containing compounds are replaced with chlorine-containing compounds, 1mL / L (v / v) of sodium lactate is further added. The anaerobic bottles were then transferred to a light incubator for light-induced hydrogen production. The culture parameters were: temperature 25°C, light duration 24h, light intensity 100μmol·m -2 ·s -1The anaerobic bottle was shaken three times a day, 0.5 mL of headspace gas was taken with a 1 mL syringe, and injected into an Agilent gas chromatograph 7890A to detect the headspace hydrogen concentration. Gas chromatography parameter settings: carrier gas was nitrogen, flow rate was 10 mL / min, and the chromatographic column was The molecular sieve column was filled with a sample inlet temperature of 70°C, a column box temperature of 60°C, and a TCD detector temperature of 100°C. The experimental period was 12 days, and the headspace gas was taken every three days to detect the hydrogen concentration. The hydrogen production results of the five groups of experiments are shown in Table 2.
[0054] Table 2. Hydrogen production of hydrogen producing microreactors with different hydrogen producing core sizes (mL / L)
[0055]
[0056] As can be seen from Table 2, the hydrogen production of the spherical algae-bacteria co-production hydrogen microreactors with different core sizes is higher than that of the suspended algae-bacteria system. Among them, the hydrogen production of the core-shell algae-bacteria co-production hydrogen microreactor with a serrated core is the highest when the particle size is 600μm, reaching 160.2ml / L on the 12th day, which is 1.82 times that of the control group, and the hydrogen production has increased significantly. And when the core particle size is 600μm, on the 12th day, the hydrogen production of the serrated core microreactor is 1.28 times that of the non-serrated core microreactor. Therefore, the serrated surface of the core can significantly provide hydrogen production.
[0057] Transfer 2mL of TAP-S' culture medium to a 10mL anaerobic glass tube, flush with argon for 10min, and then quickly plug the bottle mouth with a rubber stopper. Transfer the spherical algae co-production hydrogen microreactor to the anaerobic glass tube, and then add 1mL of reaction solution A and 200μL of reaction solution B. Reaction solution A is prepared by 10mmol / L methyl viologen, 50nmol / L Tris, and 0.02% Ttiton-X100, and reaction solution B is prepared by 0.03mol / L NaOH and 10mmol / L sodium dithionite. Reaction solutions A and B are prepared and used immediately, and are wrapped in tin foil for storage in the dark. Wrap the anaerobic glass tube containing the spherical algae co-production hydrogen microreactor with tin foil to block light and place it in a 37℃ water bath for shaking reaction for 1 hour. After the reaction was completed, the headspace gas was extracted with a gas-tight injection needle and injected into a gas chromatograph to measure the hydrogen production, and the hydrogen production per unit cell per hour of the microalgae was calculated, which was the hydrogenase activity of the microreactor. The hydrogenase activities of the six experimental groups are shown in Table 3.
[0058] Table 3. Hydrogenase activity (μmol H2 / 10 7 cells / h)
[0059]
[0060] As shown in Table 3, after multiple fixation of algae and fungi separated by space, the hydrogenase activity of the core-shell algae and fungi co-producing hydrogen microreactor was significantly higher than that of the suspension control (algae and fungi suspended growth), especially on the third day, the hydrogenase activity of the core-shell microreactor could reach a maximum of 2.65 μmol H2 / 10 7 cells / h, which is more than twice that of the control, which helps to improve the hydrogen production rate of the microreactor for hydrogen production by the nucleus shell algae. Secondly, the hydrogenase activity of the microreactor with a 600μm serrated core on the third day was 1.325 times that of the microreactor with a 600μm non-serrated core. Therefore, the serrated core helps to increase the hydrogenase activity and thus provide hydrogen production.
[0061] Finally, it should be noted that the above embodiments of the present invention are only examples for illustrating the present invention, and are not intended to limit the implementation methods of the present invention. For those skilled in the art, other different forms of changes and modifications can be made based on the above description. It is impossible to list all implementation methods here. Any obvious changes or modifications derived from the technical solution of the present invention are still within the scope of protection of the present invention.
Claims
1. A method for preparing a microreactor for enhanced hydrogen production by algae-bacteria symbiosis, characterized in that: The specific steps include: (1) mixing the hydrogen-producing microalgae and the sodium alginate solution to obtain a microalgae-sodium alginate solution, and then filling the solution into one syringe of a dual-channel microfluidic pump, and filling the calcium chloride solution into another syringe of the dual-channel microfluidic pump; (2) Connecting the dual-channel microfluidic pump to the print head of the 3D printer, and pumping out the microalgae-sodium alginate solution and the calcium chloride solution from the print head according to the preset hydrogen-producing microalgae core model, thereby printing the hydrogen-producing microalgae core of the hydrogen-producing microreactor; (3) The electroactive bacteria and the chitosan solution are mixed to obtain a bacteria-chitosan solution, and the hydrogen-producing microalgae kernels of step (2) are immersed in the bacteria-chitosan solution and stirred for 25 to 30 minutes to obtain the hydrogen-producing microreactor.
2. The method for preparing a microreactor for enhanced hydrogen production by algae-bacteria symbiosis according to claim 1, characterized in that: The hydrogen-producing microalgae is one of Chlamydomonas, Synechocystis, Scenedesmus and Chlorella.
3. The method for preparing a microreactor for enhancing hydrogen production by algae-bacteria symbiosis according to claim 1 or 2, characterized in that: In step (1), the volume ratio of hydrogen-producing microalgae to sodium alginate solution is 2.5-7.5:
1.
4. The method for preparing a microreactor for enhanced hydrogen production by algae-bacteria symbiosis according to claim 3, characterized in that: The density of hydrogen-producing microalgae is 5.0~15.0 mg chlorophyll / L; the concentration of sodium alginate solution is 10~20% (w / v); and the concentration of calcium chloride solution is 2.0~4.0% (w / v).
5. The method for preparing a microreactor for enhanced hydrogen production by algae-bacteria symbiosis according to claim 1, characterized in that: The preset hydrogen-producing microalgae kernel model includes a sphere and a plurality of cone teeth evenly distributed on the surface of the sphere, the diameter of the sphere is 200-800 μm, and the spherical surface distance between two adjacent cone teeth is 60-65 μm.
6. The method for preparing a microreactor for enhanced hydrogen production by algae-bacteria symbiosis according to claim 5, characterized in that: The diameter of the sphere is 600 μm, the bottom diameter of the cone teeth is 150 μm, the height of the cone teeth is 100 μm, the cone angle of the cone teeth is 74°, and the spherical center angle of two adjacent cone teeth is 12°.
7. The method for preparing a microreactor for enhanced hydrogen production by algae-bacteria symbiosis according to claim 1, characterized in that: In step (2), during printing, the injection flow rate of the microalgae-sodium alginate solution channel is 28-30 μL / s, the injection flow rate of the calcium chloride solution channel is 8-12 μL / s, and the print head movement speed is 45-55 μm / s.
8. The method for preparing a microreactor for enhanced hydrogen production by algae-bacteria symbiosis according to claim 1, characterized in that: The electroactive bacteria is one of Shewanella, Geobacter and Desulfomonas, and the density of the electroactive bacteria is 1.0-5.0×10 5 cells / mL.
9. The method for preparing a microreactor for enhanced hydrogen production by algae-bacteria symbiosis according to claim 1, characterized in that: In step (3), the volume ratio of the electroactive bacteria to the chitosan solution is 1:2-10; the chitosan solution has a chitosan concentration of 1.0-2.0% (w / v), and further contains acetic acid, wherein the concentration of the acetic acid is 0.5-1.0% (v / v).
10. An algae-bacteria symbiotic enhanced hydrogen production microreactor, characterized in that: The microreactor is prepared by the method for preparing an algae-bacteria symbiotic enhanced hydrogen production microreactor as described in any one of claims 1 to 9.