Animal fat powder for increasing conjugated estrogen content in pregnant mare urine, method and application

Abstract

The invention provides animal fat powder for increasing conjugated estrogen content in pregnant mare urine, a method and application, and particularly belongs to the technical field of hormone production. The invention provides animal fat powder for improving conjugated estrogen content in pregnant mare urine. The animal fat powder comprises animal fat and auxiliary materials. The animal fat powder can improve the content of conjugated estrogen in pregnant mare urine.

Description

Technical Field

[0001] The invention belongs to the technical field of hormone production, and in particular relates to an animal fat powder for increasing the content of combined estrogen in pregnant mare urine, a method and an application thereof. Background Art

[0002] In clinical practice, estrogen can be used for hormone replacement therapy, and has a good preventive and therapeutic effect on menopausal syndrome, osteoporosis and coronary heart disease. Pregnant horse urine is rich in estrogen, and the average estrogen content in pregnant horse urine is 70-130 mg / L. Each horse can produce 50-100g of estrogen during pregnancy. Conjugated estrogen is one of the biologically effective components in pregnant horse urine. It is a hormone substance formed by estrogen through glucuronidation and sulfation. Among them, sodium estrone sulfate, sodium equilin sulfate and 17-α dihydroequilin sulfate are the main ones, accounting for more than 90%. Studies have shown that the estrogen content in urine is low in early pregnancy, reaches the maximum value in mid-pregnancy, and then gradually decreases, reaching the lowest value before foaling. The synthetic pathways of saturated and unsaturated estrogens in conjugated estrogens are very different. The synthesis of estrone comes from cholesterol metabolism in the body, while the synthesis of unsaturated estrogens such as equilin is the same as cholesterol synthesis from acetyl CoA to farnesyl pyrophosphate. The synthetic route after farnesyl pyrophosphate is completely different ( Figure 1 ). At present, the research on estrogen in pregnant mare urine mainly focuses on the extraction and detection of estrogen, and there is little research on increasing the bound estrogen in pregnant mare urine. Summary of the invention

[0003] The object of the present invention is to provide an animal fat powder and a method and application thereof for increasing the content of conjugated estrogen in pregnant mare urine. The animal fat powder of the present invention can increase the content of conjugated estrogen in pregnant mare urine.

[0004] The invention provides an animal fat powder for increasing the content of combined estrogen in pregnant mare urine, comprising animal fat and auxiliary materials.

[0005] Preferably, the auxiliary material includes one or more of bran, waste flour, defatted rice bran powder, rice husk powder, corn cob powder, soybean husk powder and puffed corn powder.

[0006] Preferably, the source of the animal fat includes one or more of pig, cattle, sheep, chicken and duck.

[0007] Preferably, the animal fat content in the animal fat powder is 30-60%.

[0008] The present invention also provides the use of the animal fat powder described in the above technical solution in increasing the content of combined estrogen in pregnant mare urine.

[0009] The present invention also provides the use of the animal fat powder described in the above technical solution in increasing the content of saturated estrogen sulfate in pregnant mare urine.

[0010] The present invention also provides the use of the animal fat powder described in the above technical solution in increasing the triglyceride and total cholesterol content in the blood of pregnant horses.

[0011] The present invention also provides a method for increasing the content of combined estrogen in pregnant mare urine, comprising the following steps: supplementing the pregnant mare with the animal fat powder described in the above technical solution.

[0012] Preferably, the supplementary feeding period includes the middle and late stages of pregnancy of pregnant horses.

[0013] Preferably, the horse comprises a Kazakh horse or an Ili horse.

[0014] The present invention provides an animal fat powder for increasing the content of conjugated estrogen in pregnant mare urine, comprising chicken fat and auxiliary materials. The animal fat powder of the present invention can increase the content of conjugated estrogen in pregnant mare urine. Test results show that supplementing with animal fat powder can slow down the decline of sodium equilin sulfate, sodium estrone sulfate and the total content in pregnant mare urine in the middle and late stages of pregnancy; supplementing with animal fat powder significantly increases the content of sodium equilin sulfate, sodium estrone sulfate and the total content in pregnant mare urine; supplementing with animal fat powder significantly increases the content of triglycerides and total cholesterol in blood. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0016] Figure 1 The synthetic route of estrone and equilin provided by the present invention;

[0017] Figure 2 This is a graph showing the effect of supplementing animal fat powder provided by the present invention on the content of conjugated estrogen sulfate in the urine of pregnant horses. DETAILED DESCRIPTION

[0018] The present invention provides an animal fat powder for increasing the content of conjugated estrogen in pregnant mare urine, comprising animal fat and auxiliary materials. In a specific embodiment, the auxiliary materials may include one or more of bran, second flour, defatted rice bran powder, rice husk powder, corn cob powder, soybean husk powder and puffed corn powder. In a specific embodiment, the source of the animal fat includes one or more of pigs, cattle, sheep, chickens and ducks. Specifically, the animal fat may be one or more of lard, beef tallow, sheep fat, chicken fat and duck fat (purity ≥ 99%). In a specific embodiment, the content of animal fat in the animal fat powder may be 30-60%, specifically 50%. The animal fat powder mainly contains some lipid substances such as glycerol, long-chain fatty acids and cholesterol. After these substances enter the body, in addition to providing energy for the body, glycerol and fatty acids can also be hydrolyzed to generate acetyl CoA and short-chain fatty acids, which play a vital role in the body's metabolic process. Conjugated estrogens are one of the biologically effective components in pregnant mare urine, of which sodium estrone sulfate, sodium equilin sulfate and 17-α dihydroequilin sulfate are the main ones, accounting for more than 90%. The synthetic pathways of saturated estrogens and unsaturated estrogens in conjugated estrogens are very different. The synthesis of estrone comes from cholesterol metabolism in the body, while the synthesis of unsaturated estrogens such as equilin is the same as cholesterol synthesis from acetyl CoA to farnesyl pyrophosphate, and the synthetic route after farnesyl pyrophosphate is completely different. In a specific embodiment, the present invention starts with supplementing precursor substances such as acetyl CoA and cholesterol for synthesizing conjugated estrogens, takes Kazakh pregnant horses in the middle and late stages of pregnancy as research objects, and finds that it can increase the content of conjugated estrogens in pregnant mare urine by supplementing animal fat powder.

[0019] The present invention also provides the use of the animal fat powder described in the above technical solution in increasing the content of combined estrogen in pregnant mare urine. Supplementing animal fat powder can slow down the decline of sodium equilin sulfate, sodium estrone sulfate and the total content in pregnant mare urine in the late pregnancy; and significantly increase the sodium equilin sulfate, sodium estrone sulfate and the total content in pregnant mare urine.

[0020] The present invention also provides the use of the animal fat powder described in the above technical solution in increasing the content of saturated estrogen sulfate in pregnant mare urine. The animal fat powder can increase the content of conjugated estrogen sulfate in pregnant mare urine and slow down the trend of conjugated estrogen decreasing with the advancement of pregnancy.

[0021] The present invention also provides the use of the animal fat powder described in the above technical solution in increasing the triglyceride and total cholesterol content in the blood of pregnant horses. Triglyceride and total cholesterol are components of animal fat. The increase in the content in the blood indicates that the fat powder is better absorbed and also provides precursor substances and energy for the synthesis of sodium equilin sulfate and sodium estrone sulfate.

[0022] The present invention also provides a method for increasing the content of conjugated estrogen in pregnant horse urine, comprising the following steps: supplementing the pregnant horse with the animal fat powder described in the above technical solution. In a specific embodiment, the time of the supplementary feeding includes the middle and late stages of pregnancy of the pregnant horse. In a specific embodiment, the horse may include a Kazakh horse or an Ili horse. In a specific embodiment, the amount of the supplementary feeding is 300 to 1000 g / d / horse, specifically 400 g / d / horse. The present invention preferably feeds the animal fat powder on the basis of feeding roughage. In a specific embodiment, the roughage can be fed at 9:00, 12:00, 15:00, 20:00 and 00:00 every day. In a specific embodiment, the animal fat powder can be divided into two equal parts for feeding. In a specific embodiment, the feeding time can be 8:30 and 19:30. The present invention can collect urine of pregnant horses at 12:00 and 00:00 every day. The present invention does not specifically limit the detection method of conjugated estrogen, and a conventional detection method can be used.

[0023] To further illustrate the present invention, an animal fat powder, method and application for increasing the content of conjugated estrogen in pregnant mare urine provided by the present invention are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0024] Example 1

[0025] Experimental animals and experimental design

[0026] The experiment selected 12 Kazakh pregnant horses aged 5 to 7 years and with 2 to 4 parities, and randomly divided them into 2 groups, namely the control group and the experimental group (n = 6), respectively. From the 1st to the 21st day of the experiment, the control group and the experimental group were supplemented with corn meal 400g / d / horse, and the experimental group was supplemented with animal fat powder 400g / d / horse on this basis. The roughage fed to the experimental horses was green hay and corn stalks, with a feeding ratio of 4:1. The nutritional level of roughage is shown in Table 1.

[0027] Table 1 Roughage nutritional level (dry matter basis) (%)

[0028] project Green hay Corn stalks Dry matter 91.35 85.22 Organic matter 92.78 93.65 Crude Protein 9.72 4.6 Crude fat 2.4 1.5 Neutral detergent fiber 61.46 69.65 Acid detergent fiber 41.79 43.27

[0029] Feeding and management:

[0030] All experimental horses were housed, and roughage was fed at 9:00, 12:00, 15:00, 20:00 and 00:00 every day to ensure free feeding. After drinking water, they exercised in the playground for 3 hours every day (16:00-19:00). The corn meal and animal fat powder supplemented by the horses every day were divided into two equal parts and fed at 8:30 and 19:30 respectively to ensure clean feeding and accurate supplementary feeding amount. Urine of pregnant horses was collected at 12:00 and 00:00 every day.

[0031] result

[0032] Effect of animal fat powder supplementation on the content of conjugated estrogen sulfate in urine of pregnant horses

[0033] Effect of supplementation with animal fat powder on the content of conjugated estrogen sulfate in urine of pregnant horses Figure 2 And Table 2. Under the experimental conditions, after feeding animal fat powder for 21 days, the contents of sodium equilin sulfate, sodium estrone sulfate and total equilin sulfate decreased slowly, and the contents of sodium equilin sulfate and sodium estrone sulfate in the urine of pregnant horses were significantly increased.

[0034] Table 2 Effect of supplementing animal fat powder on the content of conjugated estrogen in pregnant mare urine (μg / mL)

[0035]

[0036]

[0037] 2 Effects of supplementing animal fat powder on blood biochemical parameters of pregnant horses

[0038] The effects of supplementing animal fat powder on blood biochemical indices of pregnant horses are shown in Table 3. Under the experimental conditions, after 21 days of supplementing animal fat powder, the levels of triglyceride and total cholesterol in the blood of pregnant horses in the experimental group were significantly higher than those in the control group, and there were no significant differences in other indicators.

[0039] Table 3 Effects of supplementing animal fat powder on blood biochemical parameters of pregnant horses

[0040]

[0041] in conclusion

[0042] In the middle and late stages of pregnancy, as the pregnancy progresses, the levels of sodium equilin sulfate, sodium estrone sulfate and the total content in the urine of pregnant horses decrease; supplementing with animal fat powder can slow down the decline in the levels of sodium equilin sulfate, sodium estrone sulfate and the total content in the urine of pregnant horses in the middle and late stages of pregnancy; supplementing with animal fat powder significantly increases the levels of triglycerides and total cholesterol in the blood of pregnant horses, and significantly increases the levels of sodium equilin sulfate, sodium estrone sulfate and the total content in the urine of pregnant horses.

[0043] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. An animal fat powder for increasing the content of conjugated estrogen in pregnant mare urine, characterized in that: Includes animal fats and trimmings.

2. The animal fat powder according to claim 1, characterized in that The auxiliary materials include one or more than two of bran, second flour, defatted rice bran powder, rice husk powder, corn cob powder, soybean husk powder and puffed corn powder.

3. The animal fat powder according to claim 1, characterized in that The sources of the animal fat include one or more of pig, cattle, sheep, chicken and duck.

4. The animal fat powder according to claim 1, characterized in that The animal fat powder contains 30-60% animal fat.

5. Use of the animal fat powder according to any one of claims 1 to 4 for increasing the content of conjugated estrogen in pregnant mare urine.

6. Use of the animal fat powder according to any one of claims 1 to 4 for increasing the content of saturated estrogen sulfate in pregnant mare urine.

7. Use of the animal fat powder according to any one of claims 1 to 4 in increasing the triglyceride and total cholesterol content in the blood of pregnant horses.

8. A method for increasing the content of conjugated estrogen in pregnant mare urine, characterized in that: The following steps are involved: Feeding the animal fat powder according to any one of claims 1 to 4 to pregnant horses.

9. The method according to claim 8, characterized in that The time for supplementary feeding includes the middle and late stages of pregnancy in pregnant horses.

10. The method according to claim 8, characterized in that The horses include Kazakh horses or Ili horses.

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