Hydrolyzed procyanidine repairing composition and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a hydrolyzed procyanidine repairing composition and a preparation method. The repairing composition is prepared from the following components in percentage by mass: 5 to 15 percent of hydrolyzed procyanidine, 0.1 to 5 percent of saccharomycetes fermentation lysate filtrate, 0.1 to 1 percent of sodium hyaluronate, 0.1 to 0.5 percent of allantoin, 1 to 5 percent of butanediol, 0.1 to 1 percent of preservative and the balance of deionized water. The hydrolyzed procyanidine is an aqueous solution with the concentration of 1%; the sodium hyaluronate comprises phenolic acid-sodium hyaluronate and dopamine-sodium hyaluronate, and the mass ratio of the phenolic acid-sodium hyaluronate to the dopamine-sodium hyaluronate is 0.5: (0.3-0.5). The repairing composition has the beneficial effects that phenolic acid and dopamine are used for modifying sodium hyaluronate, so that the moisturizing capacity and the anti-oxidation effect of the repairing composition are effectively enhanced.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a hydrolyzed proanthocyanidin repair composition and a preparation method thereof. Background Art

[0002] Proanthocyanidins are a class of flavan-3-ol compounds widely found in nature. They are polyphenolic substances formed by the polymerization of flavan-3-ol monomers such as catechin or epicatechin through C-C bonds. Proanthocyanidins have strong antioxidant capacity and can effectively eliminate superoxide anion free radicals and hydroxyl free radicals, protecting lipids from peroxidation damage. In cosmetic ingredients, sodium hyaluronate generally works together with proanthocyanidins. Sodium hyaluronate is an ideal natural moisturizing factor that can form a hydration film on the skin surface and play a role in moisturizing and skin care. Long-term use of cosmetics containing sodium hyaluronate can keep the skin moisturized, smooth, delicate, tender and elastic.

[0003] Therefore, how to effectively enhance the moisturizing effect of sodium hyaluronate in cosmetics and prepare a hydrolyzed proanthocyanidin repair composition is of great significance. Summary of the invention

[0004] The object of the present invention is to provide a hydrolyzed proanthocyanidin repair composition and a preparation method thereof to solve the problems raised in the above-mentioned background technology.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0006] A hydrolyzed proanthocyanidin repair composition comprises the following components: by mass fraction, 5-15% hydrolyzed proanthocyanidin, 0.1-5% yeast fermentation lysate filtrate, 0.1-1% sodium hyaluronate, 0.1-0.5% allantoin, 1-5% butanediol, 0.1-1% preservative, and the rest is deionized water.

[0007] More optimally, the hydrolyzed proanthocyanidins are a 1% aqueous solution; the sodium hyaluronate includes phenolic acid-sodium hyaluronate and dopamine-sodium hyaluronate, and the mass ratio of the two is 0.5:(0.3-0.5).

[0008] More optimally, the preparation method of phenolic acid-sodium hyaluronate is: mix sodium hyaluronate, ferulic acid and vitamin C, activate for 30-50 minutes under a protective atmosphere, add hydrogen peroxide, stir at room temperature for 12-15 hours, remove the supernatant, dialyze for 48-50 hours, and freeze-dry to obtain phenolic acid-sodium hyaluronate.

[0009] More optimally, the phenolic acid-sodium hyaluronate comprises the following components: by weight, 5-6 parts of sodium hyaluronate, 5-6 parts of ferulic acid, and 1-2 parts of vitamin C; the hydrogen peroxide accounts for 5-6wt% of the total mass of sodium hyaluronate, ferulic acid, and vitamin C.

[0010] More optimally, the preparation method of dopamine-sodium hyaluronate is: dissolve sodium hyaluronate in deionized water, use hydrochloric acid to adjust pH = 1-2, add 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, n-hydroxysuccinimide, and dopamine hydrochloride, stir for 24-25 hours under a protective atmosphere, transfer the reactants to a dialysis bag, dialyze for 7-8 days, and freeze-dry to obtain dopamine-sodium hyaluronate.

[0011] More optimally, the dopamine-sodium hyaluronate comprises the following components, by weight: 5-6 parts of sodium hyaluronate, 1-2 parts of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, 2-3 parts of n-hydroxysuccinimide, and 5-6 parts of dopamine hydrochloride.

[0012] More optimally, the preservative is a mixture of octanoylhydroxamic acid and p-hydroxyacetophenone, with the mass ratio of the two being 1:(2-3).

[0013] More optimally, the preparation process of hydrolyzed proanthocyanidins is as follows: S1: crush the medicinal material and add petroleum ether with a mass ratio of 1:3 to soak for 24 hours, defatting the medicinal material residue after soaking three times, combining the filtrate, and distilling under reduced pressure to obtain defatted medicinal material;

[0014] S2: Add 60% ethanol solution to the defatted medicinal material at a ratio of 1:3, extract at 30°C for 30 min, and repeat the extraction 3 times, and distill the obtained supernatant under reduced pressure to obtain a crude extract;

[0015] S3: The crude extract is distilled under reduced pressure to a specific gravity of 1.1-1.2, five volumes of ethanol are added to precipitate impurities, the filtrate is distilled under reduced pressure, and three volumes of ethyl acetate are added, extracted three times, allowed to stand for separation, and the aqueous phase is concentrated to obtain a polymer concentrate;

[0016] S4: Add the polymer concentrate to No. 1 strong acid resin in a volume ratio of 1:1, and add three times the volume of distilled water to dilute it. Under nitrogen protection, react at 70°C for 120 minutes to obtain hydrolyzed proanthocyanidins.

[0017] More optimally: the preparation process of the yeast fermentation lysate filtrate is:

[0018] Step 1: The positive colony is inoculated into a YPD medium baffle bottle and cultured at an appropriate temperature and rotation speed until the optical density (OD) 600 reaches a specific range to obtain a seed solution;

[0019] Step 2: (1) 15 L fermentation tank: Add non-selective yeast culture medium to the 15 L fermentation tank, sterilize the culture medium and defoamer to ensure a sterile environment, add the previously cultured seed liquid, and ferment at a temperature of 29 ° C, a rotation speed of 200 rpm, and a ventilation volume of 0.2 m 3 / h for 12 hours;

[0020] (2) 50 L fermenter: Add modified basal salt medium to the 50 L fermenter, sterilize the medium and defoamer, connect the PTM1 solution, and connect the seed solution in the 15 L fermenter through a transfer pipe. The temperature is 29 °C, the rotation speed is 240 rpm, and the ventilation volume is 1.2 m 3 / h, and then fed with methanol containing PTM1 for 62h; wherein, 4mL of PTM1 was added per liter of modified BSM medium, and 12mL of PTM1 was added per liter of methanol;

[0021] (3) After the fermentation is completed, the system is closed, the transfer pipes and the sedimentation tank are sterilized, and after cooling, the fermentation liquid is led to the sedimentation tank for low-temperature precipitation. The supernatant is pressed into the offset filtration equipment with an air pump. After filtration, the membrane is bubbled in a specific solution, and finally the supernatant is distilled to obtain the yeast fermentation lysate filtrate.

[0022] The preparation method of YPD medium is as follows: dissolve yeast extract, peptone and glucose in deionized water and sterilize by high pressure. Agar powder should be added to prepare solid medium, and Zeocin antibiotics should be added when screening strains;

[0023] The preparation method of the improved BSM medium is as follows: dissolve phosphoric acid, potassium sulfate, magnesium sulfate heptahydrate (or anhydrous magnesium sulfate), calcium sulfate dihydrate (or anhydrous calcium sulfate), and potassium hydroxide in deionized water in sequence, adjust the pH to 5.5 with ammonia water, and finally add peptone;

[0024] The preparation method of PTM1 is as follows: copper sulfate pentahydrate, sodium iodide, manganese sulfate monohydrate, sodium molybdate dihydrate, boric acid, cobalt chloride, zinc chloride, ferrous sulfate heptahydrate, biotin, and sulfuric acid are dissolved in deionized water in sequence.

[0025] More optimally: A method for preparing a hydrolyzed proanthocyanidin repair composition, comprising the following process: adding hydrolyzed proanthocyanidins, yeast fermentation lysate filtrate, sodium hyaluronate, allantoin, butylene glycol, a preservative, and deionized water into a container, heating to 60-70°C, stirring and mixing evenly, keeping warm and sterilizing with ultraviolet light for 40-50 minutes, to obtain a repair composition.

[0026] Compared with the prior art, the beneficial effects of the present application are as follows: the present invention uses phenolic acid and dopamine to modify sodium hyaluronate, effectively enhancing the moisturizing ability and antioxidant effect of the repair composition. The details are as follows:

[0027] First, in this scheme, phenolic acid is grafted onto sodium hyaluronate by free radical mediation, so that sodium hyaluronate and proanthocyanidins are the same, both containing a large number of phenolic hydroxyl groups as hydrogen atom donors. When free radicals approach these phenolic hydroxyl groups, the hydroxyl molecules can donate a hydrogen atom to the free radicals, converting the free radicals into a stable molecule, effectively enhancing the antioxidant properties of the repair composition.

[0028] Second: Using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide as activators, dopamine is grafted onto sodium hyaluronate. The catechol group of dopamine can form a covalent bond with the carboxyl group of sodium hyaluronate, which enhances the adsorption effect at the oil-water interface of the repair composition. At the same time, dopamine-sodium hyaluronate, as an emulsifier, can effectively reduce surface tension and form a stable emulsion, further enhancing the moisturizing ability of the repair composition. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] It should be noted that the following parts are parts by weight. The purchasing manufacturers of all raw materials involved in the present invention are not subject to any special restrictions and are exemplified as follows: in the following embodiments, sodium hyaluronate CAS is 9067-32-7, and the purchasing manufacturer is Huaxi Biology; ferulic acid CAS is 1135-24-6, and the purchasing manufacturer is Yuanye; vitamin C CAS is 50-81-7, and the purchasing manufacturer is Yuanye; 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide CAS is 1892-57-5, and the purchasing manufacturer is Alpha; n-hydroxysuccinimide CAS is 6066-82-6, and the purchasing manufacturer is Alpha; dopamine hydrochloride CAS is 62-31-7, and the purchasing manufacturer is Jusheng.

[0031] Pre-preparation of relevant raw materials: (1) The preparation process of hydrolyzed proanthocyanidins is:

[0032] S1: After crushing the medicinal materials, add petroleum ether with a mass ratio of 1:3 and soak for 24 hours, defatting the medicinal material residue after soaking three times, and combining the filtrates and performing reduced pressure distillation to obtain defatted medicinal materials;

[0033] S2: Add 60% ethanol solution to the defatted medicinal material at a ratio of 1:3, extract at 30°C for 30 min, and repeat the extraction 3 times, and distill the obtained supernatant under reduced pressure to obtain a crude extract;

[0034] S3: The crude extract is distilled under reduced pressure to a specific gravity of 1.1, five volumes of ethanol are added to precipitate impurities, the filtrate is distilled under reduced pressure, and three volumes of ethyl acetate are added, extracted three times, allowed to stand for separation, and the aqueous phase is concentrated to obtain a polymer concentrate;

[0035] S4: Add the polymer concentrate to No. 1 strong acid resin in a volume ratio of 1:1, and add three times the volume of distilled water to dilute it. Under nitrogen protection, react at 70°C for 120 minutes to obtain hydrolyzed proanthocyanidins.

[0036] (2) The preparation process of yeast fermentation lysate filtrate is as follows:

[0037] Step 1: The positive colony is inoculated into a YPD medium baffle bottle and cultured at an appropriate temperature and rotation speed until the optical density (OD) 600 reaches a specific range to obtain a seed solution;

[0038] Step 2: 15L fermenter: Add non-selective yeast culture medium to the 15L fermenter, sterilize the culture medium and defoamer to ensure a sterile environment, add the previously cultured seed liquid, and ferment at a temperature of 29°C, a speed of 200 rpm, and a ventilation volume of 0.2m 3 / h for 12 hours;

[0039] Step 3: 50L fermenter: Add modified basal salt medium to the 50L fermenter, sterilize the medium and defoamer, connect the PTM1 solution, and connect the seed solution in the 15L fermenter through the transfer line. 3 / h, and then fed with methanol containing PTM1 for 62h; wherein, 4mL of PTM1 was added per liter of modified BSM medium, and 12mL of PTM1 was added per liter of methanol;

[0040] Step 4: After the fermentation is completed, the system is closed, the transfer pipes and the sedimentation tank are sterilized, and after cooling, the fermentation liquid is led to the sedimentation tank for low-temperature precipitation. The supernatant is pressed into the offset filtration equipment with an air pump. After filtration, the membrane is bubbled in a specific solution, and finally the supernatant is distilled to obtain the yeast fermentation lysate filtrate.

[0041] The preparation method of YPD medium is as follows: dissolve yeast extract, peptone and glucose in deionized water and sterilize by high pressure. Agar powder should be added to prepare solid medium, and Zeocin antibiotics should be added when screening strains;

[0042] The preparation method of the improved BSM medium is as follows: dissolve phosphoric acid, potassium sulfate, magnesium sulfate heptahydrate (or anhydrous magnesium sulfate), calcium sulfate dihydrate (or anhydrous calcium sulfate), and potassium hydroxide in deionized water in sequence, adjust the pH to 5.5 with ammonia water, and finally add peptone;

[0043] The preparation method of PTM1 is as follows: copper sulfate pentahydrate, sodium iodide, manganese sulfate monohydrate, sodium molybdate dihydrate, boric acid, cobalt chloride, zinc chloride, ferrous sulfate heptahydrate, biotin, and sulfuric acid are dissolved in deionized water in sequence.

[0044] Example 1: A method for preparing a hydrolyzed proanthocyanidin repair composition:

[0045] Step 1: Mix 5 parts of sodium hyaluronate, 5 parts of ferulic acid, and 1 part of vitamin C, activate for 30 minutes under a nitrogen atmosphere, add hydrogen peroxide, stir at room temperature for 12 hours, remove the supernatant, dialyze for 48 hours, and freeze-dry to obtain phenolic acid-sodium hyaluronate;

[0046] Step 2: Dissolve 5 parts of sodium hyaluronate in deionized water, adjust pH to 1 with hydrochloric acid, add 1 part of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, 2 parts of n-hydroxysuccinimide, and 5 parts of dopamine hydrochloride, stir for 24 hours under nitrogen atmosphere, transfer the reactants to a dialysis bag, dialyze for 7 days, and freeze-dry to obtain dopamine-sodium hyaluronate;

[0047] Step 3: Add hydrolyzed proanthocyanidins, yeast fermentation lysate filtrate, sodium hyaluronate, allantoin, butylene glycol, preservatives, and deionized water into a container, heat to 60° C., stir and mix evenly, and keep warm for 40 minutes for UV sterilization to obtain a repair composition.

[0048] The repair composition comprises the following components: by mass fraction, 5% hydrolyzed proanthocyanidins, 0.1% yeast fermentation lysate filtrate, 0.8% sodium hyaluronate (0.5 parts of phenolic acid-sodium hyaluronate, 0.3 parts of dopamine-sodium hyaluronate), 0.1% allantoin, 1% butylene glycol, 0.1% preservative, and the rest is deionized water.

[0049] Example 2: A method for preparing a hydrolyzed proanthocyanidin repair composition, comprising the following steps:

[0050] Step 1: Mix 6 parts of sodium hyaluronate, 6 parts of ferulic acid, and 2 parts of vitamin C, activate for 50 minutes under a nitrogen atmosphere, add hydrogen peroxide, stir at room temperature for 15 hours, remove the supernatant, dialyze for 50 hours, and freeze-dry to obtain phenolic acid-sodium hyaluronate;

[0051] Step 2: Dissolve 6 parts of sodium hyaluronate in deionized water, adjust pH to 1 with hydrochloric acid, add 2 parts of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, 3 parts of n-hydroxysuccinimide, and 6 parts of dopamine hydrochloride, stir for 25 hours under nitrogen atmosphere, transfer the reactants to a dialysis bag, dialyze for 8 days, and freeze-dry to obtain dopamine-sodium hyaluronate;

[0052] Step 3: Add hydrolyzed proanthocyanidins, yeast fermentation lysate filtrate, sodium hyaluronate, allantoin, butylene glycol, preservatives, and deionized water into a container, heat to 70° C., stir and mix evenly, and sterilize under ultraviolet for 50 minutes to obtain a hydrolyzed proanthocyanidin repair composition.

[0053] The repair composition comprises the following components: by mass fraction, 15% hydrolyzed proanthocyanidins, 5% yeast fermentation lysate filtrate, 1% sodium hyaluronate (0.5 parts of phenolic acid-sodium hyaluronate, 0.5 parts of dopamine-sodium hyaluronate), 0.5% allantoin, 5% butylene glycol, 1% preservative, and the rest is deionized water.

[0054] Example 3: A method for preparing a hydrolyzed proanthocyanidin repair composition, comprising the following steps:

[0055] Step 1: Mix 5.5 parts of sodium hyaluronate, 5.5 parts of ferulic acid, and 1.5 parts of vitamin C, activate for 40 minutes under a nitrogen atmosphere, add hydrogen peroxide, stir at room temperature for 13 hours, remove the supernatant, dialyze for 49 hours, and freeze-dry to obtain phenolic acid-sodium hyaluronate;

[0056] Step 2: Dissolve 5.5 parts of sodium hyaluronate in deionized water, adjust pH to 1 with hydrochloric acid, add 1.5 parts of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, 2.5 parts of n-hydroxysuccinimide, and 5.5 parts of dopamine hydrochloride, stir for 25 hours under nitrogen atmosphere, transfer the reactant to a dialysis bag, dialyze for 8 days, and freeze-dry to obtain dopamine-sodium hyaluronate;

[0057] Step 3: Add hydrolyzed proanthocyanidins, yeast fermentation lysate filtrate, sodium hyaluronate, allantoin, butylene glycol, preservatives, and deionized water into a container, heat to 65° C., stir and mix evenly, and sterilize under ultraviolet for 45 minutes to obtain a hydrolyzed proanthocyanidin repair composition.

[0058] The repair composition includes the following components: by mass fraction, 10% hydrolyzed proanthocyanidins, 4% yeast fermentation lysate filtrate, 0.8% sodium hyaluronate (0.5 parts of phenolic acid-sodium hyaluronate, 0.3 parts of dopamine-sodium hyaluronate), 0.4% allantoin, 4% butylene glycol, 0.5% preservative, and the rest is deionized water.

[0059] Example 4: A method for preparing a hydrolyzed proanthocyanidin repair composition comprising the following steps:

[0060] Step 1: Mix 5 parts of sodium hyaluronate, 5 parts of ferulic acid, and 1 part of vitamin C, activate for 40 minutes under a nitrogen atmosphere, add hydrogen peroxide, stir at room temperature for 13 hours, remove the supernatant, dialyze for 49 hours, and freeze-dry to obtain phenolic acid-sodium hyaluronate;

[0061] Step 2: Dissolve 5.5 parts of sodium hyaluronate in deionized water, adjust pH to 1 with hydrochloric acid, add 1.5 parts of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, 2.5 parts of n-hydroxysuccinimide, and 5.5 parts of dopamine hydrochloride, stir for 25 hours under nitrogen atmosphere, transfer the reactant to a dialysis bag, dialyze for 8 days, and freeze-dry to obtain dopamine-sodium hyaluronate;

[0062] Step 3: Add hydrolyzed proanthocyanidins, yeast fermentation lysate filtrate, sodium hyaluronate, allantoin, butylene glycol, preservatives, and deionized water into a container, heat to 65° C., stir and mix evenly, and sterilize under ultraviolet for 45 minutes to obtain a hydrolyzed proanthocyanidin repair composition.

[0063] The repair composition includes the following components: by mass fraction, 10% hydrolyzed proanthocyanidins, 4% yeast fermentation lysate filtrate, 0.8% sodium hyaluronate (0.5 parts of phenolic acid-sodium hyaluronate, 0.3 parts of dopamine-sodium hyaluronate), 0.4% allantoin, 4% butylene glycol, 0.5% preservative, and the rest is deionized water.

[0064] Comparative Example 1: No phenolic acid-sodium hyaluronate was added, and the rest was the same as Example 4, specifically as follows: comprising the following steps:

[0065] Step 1: Dissolve 5.5 parts of sodium hyaluronate in deionized water, adjust pH to 1 with hydrochloric acid, add 1.5 parts of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, 2.5 parts of n-hydroxysuccinimide, and 5.5 parts of dopamine hydrochloride, stir for 25 hours under nitrogen atmosphere, transfer the reactant to a dialysis bag, dialyze for 8 days, and freeze-dry to obtain dopamine-sodium hyaluronate;

[0066] Step 2: Add hydrolyzed proanthocyanidins, yeast fermentation lysate filtrate, sodium hyaluronate, allantoin, butylene glycol, preservatives, and deionized water into a container, heat to 65° C., stir and mix evenly, and sterilize under ultraviolet for 45 minutes to obtain a hydrolyzed proanthocyanidin repair composition.

[0067] The repair composition comprises the following components: by mass fraction, 10% hydrolyzed proanthocyanidins, 4% yeast fermentation lysate filtrate, 0.8% dopamine-sodium hyaluronate, 0.4% allantoin, 4% butanediol, 0.5% preservative, and the rest is deionized water.

[0068] Comparative Example 2: Dopamine-sodium hyaluronate was not added, and the rest was the same as Example 4, specifically as follows: comprising the following steps:

[0069] Step 1: Mix 5.5 parts of sodium hyaluronate, 5.5 parts of ferulic acid, and 1.5 parts of vitamin C, activate for 40 minutes under a nitrogen atmosphere, add hydrogen peroxide, stir at room temperature for 13 hours, remove the supernatant, dialyze for 49 hours, and freeze-dry to obtain phenolic acid-sodium hyaluronate;

[0070] Step 2: Add hydrolyzed proanthocyanidins, yeast fermentation lysate filtrate, sodium hyaluronate, allantoin, butylene glycol, preservatives, and deionized water into a container, heat to 65° C., stir and mix evenly, and sterilize under ultraviolet for 45 minutes to obtain a hydrolyzed proanthocyanidin repair composition.

[0071] The repair composition comprises the following components: by mass fraction, 10% hydrolyzed proanthocyanidins, 4% yeast fermentation lysate filtrate, 0.8% phenolic acid-sodium hyaluronate, 0.4% allantoin, 4% butylene glycol, 0.5% preservative, and the rest is deionized water.

[0072] Comparative Example 3: Sodium hyaluronate purchased on the market was added, and the rest was the same as Example 4, specifically as follows: comprising the following steps:

[0073] Step 1: Add hydrolyzed proanthocyanidins, yeast fermentation lysate filtrate, sodium hyaluronate, allantoin, butylene glycol, preservatives, and deionized water into a container, heat to 65° C., stir and mix evenly, and keep warm for 45 minutes to sterilize with ultraviolet light to obtain a hydrolyzed proanthocyanidin repair composition.

[0074] The repair composition includes the following components: by mass fraction, 10% hydrolyzed proanthocyanidins, 4% yeast fermentation lysate filtrate, 0.8% sodium hyaluronate (purchased from Yingxin Laboratory, No. TX00859), 0.4% allantoin, 4% butylene glycol, 0.5% preservative, and the rest is deionized water.

[0075] Testing experiment: The repair compositions prepared in Examples 3-4 and the comparative example were tested for moisture retention using a moisture content tester; and the antioxidant properties were tested using the DPPH free radical scavenging method. The measured data are shown in the following table:

[0076] project Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Moisturizing 6.6 6.9 5.6 5.8 5.5 Free radical scavenging rate / % 91.2 91.3 74.5 75.7 74.1

[0077] Table 1

[0078] Conclusion: Examples 3-4 effectively enhance the moisturizing ability and antioxidant effect of the repair composition by modifying sodium hyaluronate with phenolic acid and dopamine. Comparative Example 1 does not add phenolic acid-sodium hyaluronate. Compared with the best example 4, the scavenging rate of free radicals and antioxidant activity decrease due to the decrease in the number of phenolic hydroxyl groups. Comparative Example 2 does not add dopamine-sodium hyaluronate. Compared with the best example 4, dopamine-sodium hyaluronate can act as a moisturizer and an emulsifier, which reduces the liquid surface tension of the repair composition and reduces its performance.

[0079] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A hydrolyzed proanthocyanidin repair composition, characterized in that: The repair composition comprises the following components: by mass fraction, 5-15% hydrolyzed proanthocyanidins, 0.1-5% yeast fermentation lysate filtrate, 0.1-1% sodium hyaluronate, 0.1-0.5% allantoin, 1-5% butylene glycol, 0.1-1% preservative, and the rest is deionized water.

2. The hydrolyzed proanthocyanidin repair composition according to claim 1, characterized in that: The hydrolyzed proanthocyanidin is a 1% aqueous solution; the sodium hyaluronate comprises phenolic acid-sodium hyaluronate and dopamine-sodium hyaluronate, and the mass ratio of the two is 0.5:(0.3-0.5).

3. A hydrolyzed proanthocyanidin repair composition according to claim 2, characterized in that: The preparation method of phenolic acid-sodium hyaluronate is as follows: sodium hyaluronate, ferulic acid and vitamin C are mixed, activated for 30-50 minutes under a protective atmosphere, hydrogen peroxide is added, stirred at room temperature for 12-15 hours, the supernatant is removed, dialyzed for 48-50 hours, and freeze-dried to obtain phenolic acid-sodium hyaluronate.

4. A hydrolyzed proanthocyanidin repair composition according to claim 3, characterized in that: The phenolic acid-sodium hyaluronate comprises the following components: by weight, 5-6 parts of sodium hyaluronate, 5-6 parts of ferulic acid, and 1-2 parts of vitamin C; the hydrogen peroxide accounts for 5-6wt% of the total weight of sodium hyaluronate, ferulic acid, and vitamin C.

5. The hydrolyzed proanthocyanidin repair composition according to claim 2, characterized in that: The preparation method of dopamine-sodium hyaluronate is as follows: dissolving sodium hyaluronate in deionized water, adjusting pH to 1-2 with hydrochloric acid, adding 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, n-hydroxysuccinimide, and dopamine hydrochloride, stirring for 24-25 hours under a protective atmosphere, transferring the reactants to a dialysis bag, dialyzing for 7-8 days, and freeze-drying to obtain dopamine-sodium hyaluronate.

6. A hydrolyzed proanthocyanidin repair composition according to claim 5, characterized in that: The dopamine-sodium hyaluronate comprises the following components, by weight: 5-6 parts of sodium hyaluronate, 1-2 parts of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, 2-3 parts of n-hydroxysuccinimide, and 5-6 parts of dopamine hydrochloride.

7. The hydrolyzed proanthocyanidin repair composition according to claim 1, characterized in that: The preservative is a mixture of octanoyl hydroxamic acid and p-hydroxyacetophenone, and the mass ratio of the two is 1:(2-3).

8. A method for preparing a hydrolyzed proanthocyanidin repair composition according to any one of claims 1 to 7, characterized in that: The method comprises the following process: adding hydrolyzed proanthocyanidins, yeast fermentation lysate filtrate, sodium hyaluronate, allantoin, butylene glycol, a preservative and deionized water into a container, heating to 60-70°C, stirring and mixing evenly, keeping warm and sterilizing with ultraviolet light for 40-50 minutes, and obtaining a repair composition.