Composition with effects of caring and relieving sensitive skin and application of composition in cosmetics

Abstract

The invention relates to a composition with effects of caring and relieving sensitive skin and application of the composition in cosmetics. The invention provides the composition containing the citrus fruit extract, the ceramide and the wild soybean seed extract, the composition can balance skin microbial flora and relieve skin, meanwhile, the sunscreen effect is remarkably improved, irritation of chemical preservatives, essence sensitization sources and irritant whitening components (such as nicotinamide) to the skin can be reduced, and the skin whitening effect is improved. Meanwhile, synergistic interaction with nicotinamide can be achieved, and the whitening effect is enhanced. The composition also has an antibacterial effect, the dosage of a preservative can be reduced, and the product safety is improved. In addition, the composition is used as a raw material to prepare a liposome microemulsion with a small particle size, and the problem that ceramide is unstable in a system is solved. The composition and the lipidosome microemulsion prepared from the composition have very good application prospects in the aspects of skin micro-ecology balancing, skin soothing, sunscreen and whitening cosmetics.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and more specifically, relates to a composition for caring for sensitive skin and soothing it, and its application in cosmetics. Background Art

[0002] Based on epidemiological surveys of sensitive skin around the world, more than 50% of people in the world have sensitive skin, which seriously affects their quality of life. At the 2017 International Itch Research Forum, international sensitive skin experts reached the following consensus: sensitive skin refers to a syndrome of discomfort caused by routine stimulation, including tingling, burning, pain, itching and tingling. These discomforts cannot be explained by any skin disease-related lesions. The skin appearance may be normal or accompanied by erythema. Sensitive skin can affect any part of the body, especially the face. Environmental factors such as pollution, radiation, and ultraviolet radiation, dietary habits, lifestyle factors such as the use of cosmetics, and endogenous factors such as personal psychological factors and emotional stress can all affect the over-activation of skin receptors, thereby causing strong discomfort to the skin. Therefore, the use of cosmetics with soothing and repairing effects to improve sensitive skin is gaining more and more attention.

[0003] In addition, with the accelerated pace of modern life, more and more consumers hope to reduce the complexity of skin care steps. If a single product can provide multiple functions, it will help simplify skin care steps and save time and cost. However, multi-functional cosmetics often mean more complex ingredients, which brings new challenges to sensitive skin. Therefore, it is of great significance to study multi-functional products suitable for people with sensitive skin to meet the needs of modern consumers for efficiency, convenience and economy. Summary of the invention

[0004] The present invention aims to provide a multifunctional composition for people with sensitive skin, which has the effects of balancing skin microbial flora, soothing skin, improving sun protection and whitening.

[0005] The first object of the present invention is to provide a composition that can balance the skin microbial flora, soothe and repair the skin, and enhance the sunscreen and whitening effects.

[0006] The second object of the present invention is to provide a composition having the effects of balancing skin microbial flora, soothing, repairing the skin and whitening the skin.

[0007] The third object of the present invention is to provide a composition having the effects of balancing skin microbial flora, soothing, repairing the skin and sun protection.

[0008] The fourth object of the present invention is to provide a composition having the effects of balancing skin microbial flora, soothing, repairing skin, sun protection and whitening.

[0009] The fifth object of the present invention is to provide a microemulsion that has the effects of balancing skin microbial flora, soothing and repairing the skin, and enhancing sun protection and whitening effects.

[0010] The sixth object of the present invention is to provide the use of the above composition or the above microemulsion.

[0011] The seventh object of the present invention is to provide a cosmetic that balances skin microbial flora, soothes skin, protects against the sun, and whitens skin.

[0012] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0013] The present invention provides a composition with the functions of balancing skin microbial flora, soothing and repairing the skin, and enhancing sunscreen and whitening effects. The components include citrus fruit extract, ceramide, and wild soybean seed extract in a mass ratio of (1-3): (0.01-0.08): (0.95-1).

[0014] Preferably, the components include citrus fruit extract, ceramide, and wild soybean seed extract in a mass ratio of (1-3): (0.03-0.08): (0.95-0.97).

[0015] More preferably, the components include citrus fruit extract, ceramide, and wild soybean seed extract in a mass ratio of 1:0.05:0.95.

[0016] The present invention provides a composition with the effects of balancing skin microbial flora, soothing, repairing skin and whitening. The components include citrus fruit extract, ceramide, wild soybean seed extract and niacinamide in a mass ratio of (1-3): (0.01-0.08): (0.95-1): (0.5-1.5).

[0017] Preferably, the components include citrus fruit extract, ceramide, wild soybean seed extract, and niacinamide in a mass ratio of (1-3): (0.03-0.08): (0.95-0.97): (0.5-1.5).

[0018] More preferably, the components include citrus fruit extract, ceramide, wild soybean seed extract, and niacinamide in a mass ratio of 1:0.05:0.95:1.

[0019] The invention provides a composition with the functions of balancing skin microbial flora, soothing, repairing skin and sun protection. The components include citrus fruit extract, ceramide, soybean seed extract, ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone and diethylamino hydroxybenzoyl hexyl benzoate in a mass ratio of (1-3): (0.01-0.08): (0.95-1): (2.5-3.5): (1.5-2.5): (1.5-2.5).

[0020] Preferably, the components include citrus fruit extract, ceramide, soybean seed extract, ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone, and diethylaminohydroxybenzoyl hexyl benzoate in a mass ratio of (1-3): (0.03-0.08): (0.95-0.97): (2.5-3.5): (1.5-2.5): (1.5-2.5).

[0021] More preferably, the components include citrus fruit extract, ceramide, soybean seed extract, ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone, and diethylaminohydroxybenzoyl hexyl benzoate in a mass ratio of 1:0.05:0.95:2:2:2.

[0022] The invention provides a composition with the effects of balancing skin microflora, soothing, repairing skin, sun protection and whitening. The components include citrus fruit extract, ceramide, wild soybean seed extract, niacinamide, ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone and diethylaminohydroxybenzoyl hexyl benzoate in a mass ratio of (1-3): (0.01-0.08): (0.95-1): (0.5-1.5): (2.5-3.5): (1.5-2.5): (1.5-2.5).

[0023] Preferably, the components include citrus fruit extract, ceramide, wild soybean seed extract, niacinamide, ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone, and diethylaminohydroxybenzoyl hexyl benzoate in a mass ratio of (1-3): (0.03-0.08): (0.95-0.97): (0.5-1.5): (2.5-3.5): (1.5-2.5): (1.5-2.5).

[0024] More preferably, the components include citrus fruit extract, ceramide, wild soybean seed extract, niacinamide, ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone, and diethylaminohydroxybenzoyl hexyl benzoate in a mass ratio of 1:0.05:0.95:1:3:2:2.

[0025] The present invention provides a microemulsion which has the functions of balancing skin microbial flora, soothing and repairing the skin, and enhancing the sunscreen and whitening effects. The microemulsion is prepared from any of the above compositions.

[0026] As an optional embodiment, the preparation method of the microemulsion is: using the above-mentioned wild soybean seed extract and ceramide as the oil phase, adding the above-mentioned citrus fruit extract to water to obtain the aqueous phase, and homogenizing the oil phase and the aqueous phase at a mass ratio of 1: (20-40) at an emulsification temperature of 60-80°C and a homogenization pressure of 60-80Mpa to form a microemulsion.

[0027] Preferably, the oil phase and the water phase are homogenized at a mass ratio of 1:30, an emulsification temperature of 70° C. and a homogenization pressure of 70 MPa.

[0028] The present invention has found that the composition of citrus fruit extract, ceramide, and wild soybean seed extract can balance the skin microbial flora, soothe and repair the skin, improve the sunscreen effect, and reduce the irritation of chemical preservatives, fragrance allergens, and some irritating whitening ingredients (such as niacinamide) to the skin. At the same time, it can synergize with niacinamide to enhance the whitening effect. The composition formula of the present invention is suitable for baby products, including premature baby products. Therefore, the present invention provides the following application schemes:

[0029] Application of the above composition or the above microemulsion in balancing skin flora, soothing skin, repairing skin barrier, sun protection and / or whitening.

[0030] Application of the above composition or the above microemulsion in preparing products for balancing skin flora, soothing skin, repairing skin barrier, sun protection and / or whitening.

[0031] Application of the above composition or the above microemulsion in reducing the skin irritation caused by chemical preservatives, fragrance allergens or niacinamide.

[0032] Application of the above composition or the above microemulsion in preparing products that reduce the skin irritation of chemical preservatives, fragrance allergens or niacinamide.

[0033] Specifically, the chemical preservative includes any one of phenoxyethanol, hexylene glycol, pentylene glycol, ethylhexylglycerin, and caprylyl glycol.

[0034] Specifically, the fragrance allergen includes any one of amyl cinnamaldehyde, amyl cinnamyl alcohol, anise alcohol, benzyl alcohol, benzyl benzoate, benzyl cinnamate, benzyl salicylate, butylphenyl methylpropional, cinnamaldehyde, cinnamyl alcohol, citral, citronellol, coumarin, eugenol, farnesol, geraniol, hexyl cinnamaldehyde, hydroxycitronellal, hydroxyisohexyl-3-cyclohexenecarboxaldehyde, isoeugenol, α-isomethyl ionone, limonene, linalool, 2-octynoic acid methyl ester, oak moss extract, and tree moss extract.

[0035] Application of the above composition or the above microemulsion in improving the whitening effect of niacinamide

[0036] The above composition or the above microemulsion is used in preparing niacinamide whitening enhancer.

[0037] In addition, the present invention provides a cosmetic that balances skin microbial flora, soothes skin, protects against sunburn, and whitens skin, and contains the above composition.

[0038] As an alternative embodiment, the cosmetic component further contains butylene glycol, glycerin, xanthan gum, ammonium acryloyldimethyltaurate / VP copolymer, dibutyl adipate, cetyl ethylhexanoate, polyglyceryl-10 stearate, polysorbate-60, sodium stearoyl lactylate, cetearyl alcohol, phenoxyethanol and water.

[0039] Optionally, the cosmetic comprises the following components in a mass ratio: 1-3% citrus fruit extract, 0.01-0.08% ceramide, 0.95-1% soybean seed extract, 0.5-1.5% niacinamide, 2.5-3.5% ethylhexyl methoxycinnamate, 1.5-2.5% diethylhexyl butyramido triazone, 1.5-2.5% diethylamino hydroxybenzoyl hexyl benzoate, 2-4% butylene glycol, 5- 7% glycerin, 0.1-0.3% xanthan gum, 0.3-0.5% ammonium acryloyldimethyltaurate / VP copolymer, 4-6% dibutyl adipate, 4-6% cetyl ethylhexanoate, 1-3% polyglyceryl-10 stearate, 0.5-1.5% polysorbate-60, 0.2-0.4% sodium stearoyl lactylate, 1-3% cetearyl alcohol, 0.5-1% phenoxyethanol, water makes up to 100%.

[0040] Preferably, the cosmetic comprises components in the following mass ratio: 1% citrus fruit extract, 0.05% ceramide, 0.95% wild soybean seed extract, 1% niacinamide, 3% ethylhexyl methoxycinnamate, 2% diethylhexyl butyramido triazone, 2% diethylamino hydroxybenzoyl hexyl benzoate, 3% butylene glycol, 6% glycerin, 0.2% xanthan gum, 0.4% ammonium acryloyldimethyltaurate / VP copolymer, 5% dibutyl adipate, 5% cetyl ethylhexanoate, 2% polyglyceryl-10 stearate, 1% polysorbate-60, 0.3% sodium stearoyl lactylate, 2% cetearyl alcohol, 0.8% phenoxyethanol, and water is supplemented to 100%.

[0041] The present invention has the following beneficial effects:

[0042] The present invention has found that the composition of citrus fruit extract, ceramide and wild soybean seed extract can balance the skin microbial flora, soothe and repair the skin, improve the sunscreen effect, reduce the irritation of chemical preservatives, fragrance allergens and some irritating whitening ingredients (such as niacinamide) to the skin, and can also synergize with niacinamide to enhance the whitening effect. The composition of the present invention also has a certain antibacterial effect, can further reduce the amount of preservatives used, improve the safety of the product, and has a good application value and prospect.

[0043] In addition, the present invention provides a liposome microemulsion with tiny particle size prepared by using wild soybean seed extract, citrus fruit extract and ceramide as raw materials, which solves the problem of instability of ceramide in the system. DETAILED DESCRIPTION

[0044] The present invention is further described below in conjunction with specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art. Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0045] The raw materials used in the following examples, including citrus fruit extract, wild soybean seed extract, ceramide NP, and niacinamide, are all commercially available.

[0046] Example 1 Determine the oil phase and high-pressure microfluidization homogenization parameters according to the three-factor four-level orthogonal experiment design

[0047] An orthogonal experiment of high-pressure microfluidic homogenization parameters was designed. The specific experimental scheme is shown in Tables 1 and 2.

[0048] Table 1 Orthogonal experimental factors and their levels of high-pressure microfluidic homogenization parameters

[0049]

[0050] Table 2 High-pressure microfluidic homogenization parameters L16(4 3 ) type orthogonal experiment table

[0051]

[0052]

[0053] Set up the processing group:

[0054] Treatment group 1: 0.05% ceramide + soybean seed extract;

[0055] Treatment group 2: 0.05% ceramide + hydrogenated lecithin;

[0056] Treatment group 3: 0.08% ceramide + soybean seed extract;

[0057] Treatment group 4: 0.10% ceramide + Glycine max seed extract.

[0058] According to the orthogonal experimental scheme shown in Table 1 and Table 2, 16 groups of experiments were conducted with deionized water as the water phase and different treatment groups as the oil phase. The experimental parameter combinations and experimental results (i.e., the measured homogeneous particle size) were listed for comparison. The results are shown in Table 3. The results show that:

[0059] (1) The high-pressure microfluidization process of wild soybean seed extract and 0.05% ceramide can form an emulsion with a particle size of 100.2-236.2 nm; however, the high-pressure microfluidization process of hydrogenated lecithin and 0.05% ceramide cannot form a uniform and stable liposome microemulsion;

[0060] (2) Under the same conditions, the higher the ceramide content added, the larger the particle size of the emulsion formed, and oil-water separation may occur.

[0061] Table 3 Experimental results

[0062]

[0063]

[0064] The results of treatment group 1 in Table 3 were subjected to range analysis, and the range analysis results are shown in Table 4. The results show that: in the mean values ​​in the analysis table, in the temperature factor, mean 1> mean 2> mean 4> mean 3; in the pressure factor, mean 1> mean 2> mean 3> mean 4; in the oil phase to water phase mass ratio factor, mean 1> mean 2> mean 4> mean 3. Therefore, the optimal parameter combination of high-pressure microjet emulsification homogenization for preparing microemulsions with 0.05% ceramide, wild soybean seed extract and deionized water is 70°C temperature, 80MPa homogenization pressure, and 30:1 water phase to oil phase mass ratio. The microemulsion obtained by the process parameters of high-pressure microjet homogenization has the smallest particle size and the best sample stability.

[0065] Table 4 Range analysis results

[0066]

[0067]

[0068] Example 2 Preparation of microemulsion by combining citrus fruit extract, ceramide and soybean seed extract

[0069] The process parameters of high-pressure microfluidic homogenization were set as follows: temperature 70°C, homogenization pressure 80 MPa.

[0070] Microemulsions were prepared according to the formulas of compositions 1-10 shown in Table 5. The preparation method was as follows:

[0071] S1. Heat deionized water to 90°C and keep it warm for 10 minutes; cool it down to 70°C; and pump it into a high-pressure microfluidizer;

[0072] S2. Add the oil phase into a high-pressure microfluidizer, set the temperature to 70°C and the homogenization pressure to 80 MPa, and perform homogenization for 10 min;

[0073] S3. After confirming that the homogenized sample is evenly dispersed, cool it to 50°C, add citrus fruit extract or 4-tert-butyl cyclohexanol (4-tert-butyl cyclohexanol has a soothing effect on the skin), and stir evenly;

[0074] S4. When the temperature drops to 40°C, take samples and test them. If they are qualified (the qualified standard is that the sample has no oil-water separation and is in a uniform phase state), filter the material with a 500-mesh filter.

[0075] Microemulsions 1-10 were prepared, and microemulsion 10 was used as a positive control group.

[0076] Table 5: Weight parts of each component of composition 1-10

[0077]

[0078] Example 3 Test on the effect of balancing skin microbial flora by a combination of citrus fruit extract, ceramide, and soybean seed extract

[0079] In this example, microemulsions 1-10 prepared in Example 2 were used as test samples, and 30 subjects aged between 18 and 45 were tested. The skin types of all the subjects had a tendency to inflammatory reactions.

[0080] The subjects were divided into 10 groups, 3 in each group, to participate in a double-blind randomized clinical study in accordance with the ethical principles of medical research. The subjects in each group used 1-10 samples of microemulsion on their faces every morning and evening, 0.5 mL each time. 28 ), detect the skin microbial community, specifically:

[0081] Thirty facial skin samples were collected non-invasively through skin patches, and microbial 16SrRNA sequencing and metagenomic analysis were performed to analyze the structure and abundance of the biological flora. In order to obtain the species classification information corresponding to each OTU, the RDP classifier Bayesian algorithm was used to perform taxonomic analysis on the OTU representative sequences at a similarity level of 97%, and the community composition of each sample was statistically analyzed at each taxonomic level: domain, kingdom, phylum, class, order, family, genus, and species.

[0082] All sequences were divided into OTUs (Operational Taxonomic Units) according to 97% similarity and subjected to bioinformatics statistical analysis. Based on the results of taxonomic analysis, the taxonomic comparison of one or more samples at each taxonomic level can be obtained. The data is now displayed through the changes in the relative abundance of species in the bacterial community structure.

[0083] 16S rRNA sequencing standard operating procedures:

[0084] (1) Extract DNA using QIAamp DNA extraction kit and detect the DNA;

[0085] (2) The concentration of DNA was measured by fluorescence spectrophotometer, and the quality of DNA was measured by 1% agarose gel electrophoresis;

[0086] (3) Adjust the concentration of DNA solution. The DNA working solution is stored at 4°C and the storage solution is stored at -20°C.

[0087] (4) PCR amplification of the V3-V4 region of the sample 16S rRNA gene;

[0088] (5) Gel recovery and purification: Gel recovery is performed on the target band to obtain a purified sample;

[0089] (6) Quantification of each sample: Quantify each sample using a Qubit fluorescence quantifier;

[0090] (7) The standard Illumina TruSeq DNA library preparation experimental process (Illumina TruSeq DNA Sample Preparation Guide) was used to construct the machine library; and the machine sequencing was performed using Illumina MiSeq PE300.

[0091] In order to obtain the species classification information corresponding to each OTU (Operational Taxonomic Units), the RDP classifier Bayesian algorithm was used to perform taxonomic analysis on the OTU representative sequences with a similarity level of 97%, and the community composition of each sample was statistically analyzed at each taxonomic level: domain, kingdom, phylum, class, order, family, genus, and species. All sequences were divided into OTUs (Operational Taxonomic Units) according to the 97% similarity and subjected to bioinformatics statistical analysis. According to the results of the taxonomic analysis, the taxonomic comparison of one or more samples at each taxonomic level can be obtained. The data is now displayed through the changes in the relative abundance of species in the bacterial community structure.

[0092] The relative abundance of species in the bacterial community structure in healthy facial skin (data from the literature "Li Z, Xia J, Jiang L, et al. Characterization of the human skin resistome and identificationof two microbiota cutotypes. Microbiome. 2021Feb 17; 9(1):47.") is shown in Table 6.

[0093] After using microemulsions prepared with different compositions on the face, the changes in the relative abundance of species in the structure of the skin flora are shown in Table 7. The results show that the skin microbial flora analysis by facial sampling shows that microemulsions 4 to 9 have the effect of balancing the skin microbial flora, and can restore the disordered microbial flora state of damaged skin to a relatively stable and balanced skin microecological flora state close to that of healthy skin, among which microemulsion 5 has the best effect. Composition 10 has no effect of balancing the skin microbial colony.

[0094] Table 6 Relative abundance of species in the bacterial community structure of healthy facial skin

[0095] Propionibacterium 0.600 Acinetobacter 0.002 Corynebacterium 0.102 staphylococcus 0.050 Bacteroides 0.035 Prevotella 0.028 Weissella 0.038 Bifidobacterium 0.005 Lactobacilli 0.025 Aquatic fungi 0.005

[0096] Table 7 Changes in relative abundance of skin flora structure and genus species after using different microemulsions on the face

[0097]

[0098]

[0099] Example 4 Skin repairing effect of a combination of citrus fruit extract, ceramide, and soybean seed extract

[0100] For the subjects who used microemulsion samples 1-10 on their faces every morning and evening in Example 3, the test results were analyzed before the test started on the same day (D0) and on the 28th day after the test (D 28 ), detect the water content of the skin stratum corneum and the TEWL value of the skin.

[0101] (1) Test of moisture content of stratum corneum

[0102] The measurement of skin moisture is based on the internationally recognized Corneometer method, which is the capacitance method. The capacitance method is based on the fact that the dielectric constant of water and other substances is completely different. The skin is a weakly conductive medium, and its conductivity increases with the water content of the skin. Therefore, when the test probe acts on the skin, the capacitor shows a change in capacitance according to the moisture content of the skin.

[0103] The stratum corneum moisture content of the subjects using different compositions was tested using a skin moisture tester Corneometer CM825. The larger the measured value, the higher the stratum corneum moisture content, and the unit is CU.

[0104] The test results of stratum corneum moisture content are shown in Table 8. The results show that compared with the baseline value before use (D0), the moisture content of the stratum corneum of the skin increased after the subjects used microemulsions 3-9 in the morning and evening for 28 days. Among them, the moisture content of the stratum corneum of the skin in the microemulsion 5, microemulsion 7 and microemulsion 8 groups increased most significantly, with extremely significant differences compared with the baseline value (p < 0.01).

[0105] Table 8 Test results of stratum corneum water content

[0106]

[0107]

[0108] (2) Transepidermal Water Loss (TEWL)

[0109] The trend of TEWL value reduction represents the recovery process of transepidermal water loss of barrier-damaged skin. By comparing the changes in the average TEWL value of skin before and after using the product, the effect of the product in reducing skin water loss can be reflected. The more the average TEWL value at different revisit time points decreases compared with the baseline value before use (D0), the better the product repair effect.

[0110] Using Tewameter TM Hex 300 tests the skin of the subjects. The larger the TEWL value, the more transepidermal water loss per unit time and unit cross-sectional area, and vice versa. The unit is g / (hm 2 ).

[0111] The results are shown in Table 9. The results show that, through the analysis of the TEWL value of the skin, compared with the baseline value before use (D0), the average value of the TEWL of the skin decreased after the subjects used microemulsion 3-9 in the morning and evening for 28 days. Among them, the average value of the TEWL of the skin in the microemulsion 5-9 group showed the most significant downward trend, and there was a very significant difference compared with the baseline value (p < 0.01).

[0112] Table 9 TEWL value measurement results

[0113]

[0114]

[0115] Experiments show that the combination of citrus fruit extract, ceramide, and wild soybean seed extract has a significant skin barrier repair effect, while the combination of 4-tert-butyl cyclohexanol, ceramide, and wild soybean seed extract has no skin barrier repair effect.

[0116] Example 5 The composition of citrus fruit extract, ceramide and soybean seed extract has a skin soothing effect

[0117] For the subjects who used microemulsion samples 1-10 on their faces every morning and evening in Example 3, the test results were analyzed before the test started on the same day (D0) and on the 28th day after the test (D 28 ), detect the average value of facial skin erythema area, the specific method is:

[0118] The red area images taken by the VISIA-CR instrument can be analyzed by the color spot analysis in the comprehensive skin analysis module of the Image Pro Plus software to measure the change (%) of the skin red area area in the AOI selection area (radius 3.5 cm) at different time points. By comparing the change of the skin red area area before and after use, the soothing effect of the composition can be reflected.

[0119] Change rate of red area ratio = (red area ratio after using the composition - red area ratio before using the composition) / red area ratio before using the composition × 100%

[0120] The results of the numerical detection of the facial skin erythema area are shown in Table 10: Through the analysis of the skin red area, after the subjects used microemulsions 1-10 in the morning and evening for 28 days, the skin red area ratio decreased. Among them, the downward trend of microemulsions 5-8 groups was the most significant, and compared with the baseline value before use (D0), there were extremely significant differences (p < 0.01), indicating that the combination of citrus fruit extract, ceramide, and wild soybean seed extract has a soothing effect on the skin.

[0121] Table 10 Numerical detection results of facial skin erythema area

[0122]

[0123]

[0124] Example 6 Composition of citrus fruit extract, ceramide, and soybean seed extract to reduce the amount of preservatives added

[0125] Taking phenoxyethanol, a conventional preservative with antibacterial effect, as an example, the effect of a combination of citrus fruit extract, ceramide, and wild soybean seed extract on the preservative performance after compounding with the preservative was tested.

[0126] (I) Preparation of microemulsion 11-21

[0127] Microemulsions were prepared according to the formulas of compositions 11-21 shown in Table 1. The preparation method was as follows:

[0128] S1. Heat deionized water to 90°C and keep it warm for 10 minutes; cool it down to 70°C; and pump it into a high-pressure microfluidizer;

[0129] S2. Add the oil phase into a high-pressure microfluidizer, set the temperature to 70°C and the homogenization pressure to 80 MPa, and perform homogenization for 10 min;

[0130] S3. After confirming that the homogenized sample is evenly dispersed, cool it to 50°C, add citrus fruit extract or 4-tert-butyl cyclohexanol, and preservatives, and stir evenly;

[0131] S4. When the temperature drops to 40℃, take samples and filter the material with a 500-mesh filter after passing the test.

[0132] Microemulsion samples 11-21 were prepared.

[0133] Table 11 Formula of each component in weight of composition 11-21

[0134]

[0135]

[0136] (II) Antiseptic efficacy test of microemulsion 11-21

[0137] The experiment was conducted according to the test method for the preservative efficacy of water-soluble cosmetics CTFA M-3 (2007), specifically:

[0138] a. Preparation of bacterial suspension: Inoculate Staphylococcus aureus ATCC6538, Pseudomonas aeruginosa ATCC9027 and Escherichia coli ATCC8739 on soybean casein agar slant medium respectively and culture at 37℃ for 24h. Rinse the bacterial lawn with 5mL sterilized 0.85% saline and determine the number of bacterial colonies according to the total colony count determination method (refer to national standard method GB 4789.2). Ensure that the number of colonies is within 10 8 CFU / mL, mix the above three bacterial suspensions in equal volumes and keep at 4℃ for later use.

[0139] b. Preparation of fungal suspension: Candida albicans ATCC10231 and Aspergillus niger ATCC16404 were inoculated on potato dextrose agar slant medium, cultured at 28°C for 48h, rinsed with 5mL sterilized 0.85% saline, and tested the colony count of the fungus according to the total colony count determination method to ensure that the colony count was within 10 7CFU / mL; culture Aspergillus niger at 28℃ for 5-7 days, take 5mL of sterilized 0.85% saline containing 0.05% Tween to rinse the bacterial moss, and test the colony count of the fungus according to the total colony count test method to ensure that the colony count is within 10 7 CFU / mL. Mix the two fungal suspensions in equal volumes and store at 4℃ for later use.

[0140] c. Sample inoculation: Take 2 samples of microemulsion 11-21 (30 g each), add 0.3 mL of mixed bacterial suspension and 0.3 mL of mixed fungal suspension respectively, and mix well. Store the samples at room temperature (22.5±2.5)℃, and test the colony count in microemulsion 11-21 on days 0, 7, 14, 21, and 28 to determine the preservative efficacy of microemulsion 11-21.

[0141] d. Sample testing: Weigh 1g or 1mL of sample, add it to a 9mL sterile saline dilution bottle, shake it thoroughly to make a 1:10 sample dilution, then take 1mL of this dilution and add it to 9mL of saline, mix it thoroughly to make a 1:100 sample dilution, and then make 1:1000, 1:10000, etc. by analogy, and dilute to the appropriate multiple. According to the results of each experiment, select 2mL of the dilution with the appropriate dilution, add it to 2 sterilized culture dishes, and add 1mL to each culture dish. The microemulsion with bacterial suspension is counted after being cultured on soybean casein agar medium at 37℃ for 48h; the microemulsion with fungal suspension is counted after being cultured on potato dextrose agar medium at 28℃ for 72h.

[0142] Result counting: Counting was performed according to the colony counting method in the national standard GB 7918.2-87.

[0143] Evaluation criteria: The antiseptic effect of microemulsion is evaluated by the percentage of colony count reduction.

[0144]

[0145] Where: A0 is the content of microorganisms in the microemulsion when the microorganisms are initially added; An is the content of microorganisms in the microemulsion at different detection times.

[0146] The total number of bacteria colonies decreased by 99.9% on the 7th day and did not increase on the 28th day, and the number of fungi decreased by 99.0% on the 14th day and did not increase on the 28th day, which was considered to be a qualified preservation effect. If one of the bacteria and fungi could not meet the above standards, the microemulsion was considered to have an unqualified preservation effect.

[0147] The results of the colony concentration of the microemulsion at different culture days are shown in Table 12. The results show that through the antiseptic challenge test, the composition of citrus fruit extract, ceramide, and wild soybean seed extract can reduce the amount of preservatives added while maintaining good antiseptic function.

[0148] Table 12 Colony concentration results of microemulsions 11-21 at different culture days

[0149]

[0150]

[0151] Example 7 Combination of citrus fruit extract, ceramide, and soybean seed extract can reduce the irritation of preservatives

[0152] Taking phenoxyethanol, a conventional preservative with antibacterial effect, as an example, the effect of a composition of citrus fruit extract, ceramide, and wild soybean seed extract on the irritation of preservatives was tested.

[0153] (I) Preparation of microemulsion 22-31

[0154] Microemulsions were prepared according to the formulations of compositions 22-31 shown in Table 13. The preparation method was the same as that in Example 6 to obtain microemulsions 22-31.

[0155] Table 13 Formula of each component in weight of composition 22-31

[0156]

[0157] (II) Phenoxyethanol irritation reduction test

[0158] Phenoxyethanol is the most commonly used preservative in cosmetics and can be used as a representative preservative irritant to verify the efficacy of anti-irritant microemulsion. A phenoxyethanol irritation model was constructed, with 1% phenoxyethanol as the irritant, and 30 subjects with adverse reactions to phenoxyethanol were screened for the test.

[0159] The 30 subjects were divided into 10 groups, with 3 subjects in each group. The subjects applied 0.5 mL of microemulsion samples 22-31 to the right nasolabial fold, and scored the tingling and burning sensations 2-8 minutes after application, with scores ranging from 0 to 3, where 0 represents no tingling or burning; 1 represents mild tingling or burning; 2 represents moderate tingling or burning; and 3 represents strong tingling or burning.

[0160] (III) Experimental results

[0161] The results of sensory irritation scores of different microemulsion treatments are shown in Table 14. The results show that through the phenoxyethanol irritation test, the citrus fruit extract, ceramide, and wild soybean seed extract composition can reduce the irritation of phenoxyethanol to the skin.

[0162] Table 14 Irritation sensory scores of microemulsions 22-31

[0163]

[0164] Example 8 Combination of citrus fruit extract, ceramide, and soybean seed extract can reduce the irritation of preservatives

[0165] Taking the compound polyol (hexanediol, pentylene glycol, ethylhexylglycerin, caprylyl glycol) preservative with antibacterial effect as an example, the effect of the composition of citrus fruit extract, ceramide, and wild soybean seed extract on the irritation of the preservative was tested.

[0166] (I) Preparation of microemulsion 32-41

[0167] Microemulsions were prepared according to the formulations of compositions 32-41 in Table 15. The preparation method was the same as that in Example 6 to obtain microemulsions 32-41.

[0168] Table 15: Weight parts of each component of composition 32-41

[0169]

[0170]

[0171] (II) Polyol stimulation reduction test

[0172] In cosmetic products, in order to cater to the concept of no preservatives, polyol raw materials are used for preservatives in product formulas. In order to achieve the preservative effect, the product needs to add a higher amount of polyols, and a high amount of polyols will cause corresponding irritation to the skin. The polyols used include pentanediol, hexanediol, caprylyl glycol, ethylhexylglycerin, etc. A polyol irritation experimental model is now constructed, and 30 subjects with adverse reactions to polyols are screened for testing to evaluate the effect of the test samples on alleviating the burning and tingling sensations caused by polyols acting on the skin.

[0173] The 30 subjects were divided into 10 groups, with 3 subjects in each group. The subjects applied 0.5 mL of microemulsion 32-41 samples to the right nasolabial fold, and scored the tingling and burning sensations 2-8 minutes after application, with scores ranging from 0 to 3, where 0 represents no tingling or burning; 1 represents mild tingling or burning; 2 represents moderate tingling or burning; and 3 represents strong tingling or burning.

[0174] (III) Experimental results

[0175] The irritation sensory scores of microemulsion 32-41 treatment are shown in Table 16. The results show that:

[0176] Through the polyol irritation test, the citrus fruit extract, ceramide, and soybean seed extract composition can reduce the irritation of polyol to the skin.

[0177] In summary, the combination of 4-tert-butyl cyclohexanol, ceramide, and wild soybean seed extract has a certain anti-preservative irritation effect, but is not as good as the combination of citrus fruit extract, ceramide, and wild soybean seed extract.

[0178] Table 16 Irritation sensory scores of microemulsions 32-41

[0179]

[0180] Example 9 Composition of citrus fruit extract, ceramide, and soybean seed extract can reduce the stimulation of fragrance allergens

[0181] (one) 3D full-thickness reconstructed human skin model

[0182] The 3D skin model shows the typical morphology and growth characteristics of natural human skin. It is formed based on a special dermal collagen matrix, on which human fibroblasts, which are key to skin growth, are seeded, forming a dermis equivalent to that of a living body after a few days.

[0183] All differentiation markers produced by the full-thickness skin model, such as cytokeratin-1 / 10, filaggrin, transglutaminase, laminin, elastin, and fibronectin, are similar to those produced by natural human skin. Representative inflammatory markers (interleukins and cytokines) produced and secreted by the full-thickness skin model extracted from the culture supernatant can be used for inflammatory response studies. In addition, cell viability and cell death can also be analyzed using this model.

[0184] This study uses a 3D full-thickness skin model. By designing a 3D skin model, the model is highly similar to human skin in terms of structure, morphology, tissue environment and physiological characteristics, and can realize the physiology and reactivity of the skin. It is an ideal model for studying the interaction between the epidermis and dermis. This experiment characterizes the level of skin irritation by the allergen through the content of interleukin-6.

[0185] (II) Experimental methods to reduce the stimulation of fragrance allergens

[0186] Tissue culture: 3D full-thickness skin model (Henkel, Germany).

[0187] Test sample preparation: 26 allergens were diluted to 1% concentration with olive oil, and 100% pure olive oil was used as the control.

[0188] Operation method: Use microemulsion 1-10 to pre-treat the 3D skin model, then apply 26 kinds of fragrance allergens and 100% pure olive oil for stimulation, and then detect the interleukin-6 content of the 3D skin model stimulated by the allergens pre-treated with microemulsion 1-10 after 24 hours.

[0189] (III) Experimental results

[0190] The interleukin-6 content results of the 3D skin model are shown in Table 17. The results show that the combination of citrus fruit extract, ceramide, and wild soybean seed extract can significantly reduce the expression of interleukin-6 caused by 26 allergens in the fragrance, reduce the degree of skin inflammation, and reduce the irritation caused by the fragrance.

[0191] Table 17 Interleukin-6 content of 3D skin model stimulated by allergens pre-treated with microemulsions 1-10

[0192]

[0193]

[0194]

[0195]

[0196] Example 10 Composition of citrus fruit extract, ceramide, and soybean seed extract can reduce the irritation of whitening agent niacinamide

[0197] (I) Preparation of microemulsion 42-51

[0198] Microemulsions were prepared according to the formulas of compositions 42-51 in Table 18. Preparation method:

[0199] S1. Heat deionized water to 90°C and keep it warm for 10 minutes; cool it down to 70°C; and pump it into a high-pressure microfluidizer;

[0200] S2. Add the oil phase into a high-pressure microfluidizer, set the temperature to 70°C and the homogenization pressure to 80 MPa, and perform homogenization for 10 min;

[0201] S3. After confirming that the homogenized sample is evenly dispersed, cool it to 50°C, add citrus fruit extract or 4-tert-butyl cyclohexanol and niacinamide, and stir evenly;

[0202] S4. When the temperature drops to 40℃, take samples and filter the material with a 500-mesh filter after passing the test.

[0203] Microemulsion samples 42-51 were prepared.

[0204] Table 18 Formula of each component in weight of composition 42-51

[0205]

[0206]

[0207] (II) Test to reduce the irritation of whitening agent niacinamide

[0208] A niacinamide stimulation model was constructed, and niacinamide was used as the stimulation source. 30 subjects who had adverse reactions to niacinamide (including skin flushing, itching, burning sensation, and tingling) were screened for the experiment.

[0209] The 30 subjects were evenly divided into 9 groups, with 3 subjects in each group. Each group of subjects applied 0.5 mL of the microemulsion 42-51 sample on their face for 2-8 minutes, and rated the stinging and burning sensations through self-evaluation; the skin flushing score was evaluated by the doctor, with the score ranging from 0 to 3 points.

[0210] Skin irritation score: 0 points represent no irritation; 1 point represents mild irritation; 2 points represent moderate irritation; 3 points represent strong irritation.

[0211] Skin burning score: 0 points represent no burning; 1 point represents mild burning; 2 points represent moderate burning; 3 points represent strong burning.

[0212] Skin flushing score: 0 points represent no flushing; 1 point represents mild flushing; 2 points represent moderate flushing; 3 points represent strong flushing.

[0213] (III) Test results

[0214] The mean scores of skin irritation reactions in the nicotinamide stimulation model are shown in Table 19. The results show that:

[0215] Through the niacinamide irritation test, the combination of citrus fruit extract, ceramide, and wild soybean seed extract can reduce the irritation of niacinamide to the skin.

[0216] Table 19 Mean scores of skin irritation reactions in nicotinamide stimulation model

[0217]

[0218]

[0219] Example 11 The composition of citrus fruit extract, ceramide and wild soybean seed extract has the effect of synergizing niacinamide to improve the whitening effect

[0220] (I) Preparation of microemulsion 52-61

[0221] Microemulsions were prepared according to the formulas of compositions 52-61 in Table 20. Preparation method:

[0222] S1. Heat deionized water to 90°C and keep it warm for 10 minutes; cool it down to 70°C; and pump it into a high-pressure microfluidizer;

[0223] S2. Add the oil phase into a high-pressure microfluidizer, set the temperature to 70°C and the homogenization pressure to 80 MPa, and perform homogenization for 10 min;

[0224] S3. After confirming that the homogenized sample is evenly dispersed, cool it to 50°C, add citrus fruit extract or 4-tert-butyl cyclohexanol and niacinamide, and stir evenly;

[0225] S4. When the temperature drops to 40℃, take samples and filter the material with a 500-mesh filter after passing the test.

[0226] Microemulsion samples 52-61 were prepared.

[0227] Table 20 Formula of each component in weight of composition 52-61

[0228]

[0229] (II) Experiment on improving whitening effect by cooperating with niacinamide

[0230] This study tested subjects aged 18-45 years old, and screened 30 subjects with darker facial skin color, divided them into 10 groups, 3 people in each group, and each group of subjects applied 0.5mL of microemulsion 47-55 samples on their faces in the morning and evening every day. 28 ), respectively testing facial skin melanin, pigment data collection and facial image analysis.

[0231] The skin color difference test probe and multifunctional skin test system (CL400 and MPA6, CK, Germany) and the skin red-melanin tester and test probe (Mexameter MX18, CK, Germany) were used to evaluate the changes in skin brightness (brightness) and skin melanin content before and after the subjects used microemulsion samples 52-61, so as to determine the whitening effect of each sample.

[0232] Skin melanin content MI value: Based on the principle of spectral absorption (RGB), the melanin content in the skin is determined by measuring the radiation after a specific wavelength of light is irradiated on the human skin. The measurement range of the instrument is 0-999. The higher the measured value, the higher the melanin content in the skin.

[0233] Test results: △MI=Tn -T0, where

[0234] T n ——The value of melanin content in the tested area changing over time;

[0235] T0——the starting value of melanin content in the tested area;

[0236] T0、T n Represents the amount of melanin per unit area. The larger the value, the darker the skin color.

[0237] Skin brightness L* value: The brightness L* value is used to characterize white balance. The larger the L* value, the more the color tends to white, and vice versa, the more the color tends to black.

[0238] Test results: △L*=t n -t0, where

[0239] t n ——The brightness of the tested area changes over time;

[0240] t0——The starting value of the brightness of the test area.

[0241] (III) Experimental results

[0242] The results of the numerical determination of skin melanin content are shown in Table 21, and the results of the numerical determination of skin brightness are shown in Table 22. The results show that through the analysis of skin melanin MI value and skin brightness L* value, the combination of citrus fruit extract, ceramide, and wild soybean seed extract with the whitening ingredient niacinamide has a synergistic effect with niacinamide, improving the whitening effect of niacinamide. However, the combination of 4-tert-butyl cyclohexanol, ceramide, and wild soybean seed extract does not have a synergistic effect with niacinamide.

[0243] Table 21 Numerical determination results of skin melanin content

[0244]

[0245]

[0246] Table 22 Skin brightness numerical measurement results

[0247] sample <![CDATA[Brightness value of D0 t0 before use]]> <![CDATA[Using for 28 days D 28 t 28 Brightness value]]> <![CDATA[△L*=t 28 -t0]]> Microemulsion 52 63.46 64.01 0.55 Microemulsion 53 63.24 64.02 0.78 Microemulsion 54 63.59 64.88 1.29 Microemulsion 55 63.41 64.91 1.50 Microemulsion 56 63.27 66.78 3.51 Microemulsion 57 63.28 64.79 1.51 Microemulsion 58 63.33 66.23 2.90 Microemulsion 59 63.56 66.56 3.00 Microemulsion 60 63.48 64.92 1.44 Microemulsion 61 63.56 64.02 0.46

[0248] Example 12 Composition of citrus fruit extract, ceramide and wild soybean seed extract has sunscreen synergistic effect (I) Preparation of microemulsion 62-71

[0249] Microemulsions were prepared according to the formulas of compositions 62-71 in Table 23. Preparation method:

[0250] S1. Add deionized water, butylene glycol, glycerol, xanthan gum, and ammonium acryloyldimethyltaurate / VP copolymer into an emulsifying pot, stir and heat to 85-88°C, stir until the materials are mixed and dispersed evenly, keep warm, and set aside;

[0251] S2. Add ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone, diethylamino hydroxybenzoyl hexyl benzoate, dibutyl adipate, and cetyl ethylhexanoate into the oil phase pot, stir and heat to 88-92°C, stir until the material is mixed and dispersed evenly; cool to 85°C, add polyglycerol-10 stearate, polysorbate-60, sodium stearoyl lactylate, and cetearyl alcohol, stir evenly, and obtain an oil phase material;

[0252] S3. Slowly add the mixed oil phase material into the emulsifier, and then homogenize at 3000r / min for 5-10 minutes until the material is evenly mixed and dispersed;

[0253] S4. Cool down to 45°C, add phenoxyethanol and microemulsion 1-10, stir evenly and continue to cool;

[0254] S5. Cool down to 38℃, take samples and filter out the material with a 500-mesh filter after passing the test.

[0255] Microemulsion 62-71 samples were prepared.

[0256] Table 23 Mass percentage formula of each component of composition 62-71

[0257]

[0258]

[0259] (II) Determination of sun protection value SPF and sun protection value PFA

[0260] Light source: Daylight simulator xenon arc lamp, all performance items meet the measurement specification requirements.

[0261] Test method:

[0262] (1) Conduct the test with reference to the specific requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition). The subject lies prone and the back is exposed to the radiation. The minimum erythema dose (MED value) of the subject's skin to ultraviolet radiation is predicted 24 hours before the test, and the ultraviolet radiation dose is adjusted according to the predicted results for the test sample. On the day of the test, a spot not less than 30 cm above the subject's back is first selected. 2 Normal skin area, (2.00±0.05)mg / cm 2Apply the sample or standard reference substance evenly to the above area, wait for 15 minutes or follow the instructions on the label. Then select the appropriate UV irradiation dose and irradiate in three conditions: ① No sample is applied to the subject's skin; ② Apply the standard reference sample; ③ Apply the sample to be tested (microemulsion 62-71). Observe the experimental results after 24 hours and record the MED values ​​in the three conditions.

[0263] The SPF value of a sample or standard product protecting a single subject is expressed as follows: Calculate the arithmetic mean of the SPF values ​​of all subjects protected by the sample, and take the integer part as the SPF value of the measured sample.

[0264] (2) Conduct the test with reference to the specific requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition). The subject lies in a prone position and the back is exposed to the radiation. The minimum sustained darkening amount (MPPD value) of the subject's skin to ultraviolet radiation is predicted 24 hours before the test, and the ultraviolet radiation dose is adjusted according to the predicted results for the test sample. On the day of the test, a spot not less than 30 cm above the subject's back is selected. 2 Normal skin area, (2.00±0.05)mg / cm 2 Apply the sample or standard reference substance evenly to the above area, wait for 15 minutes or follow the instructions on the label. Then select the appropriate UV irradiation dose and irradiate in three conditions: ① No sample is applied to the subject's skin; ② Apply the standard reference sample; ③ Apply the sample to be tested (microemulsion 62-71). Observe the results after 2 to 4 hours and record the MPPD values ​​in the three conditions.

[0265] The PFA value of a sample or standard protecting a single subject is expressed as follows: Calculate the arithmetic mean of the PFA values ​​of all subjects protected by the sample, and take the integer part as the PFA value of the measured sample.

[0266] (III) Experimental results

[0267] The results of the determination of the sunscreen human body values ​​SPF and PFA of microemulsion 62-71 are shown in Table 24. The results show that:

[0268] Through SPF and PFA value analysis, the combination of citrus fruit extract, ceramide, and wild soybean seed extract has a sunscreen synergistic effect when used in a sunscreen system, while the combination of 4-tert-butyl cyclohexanol, ceramide, and wild soybean seed extract does not have a sunscreen synergistic effect.

[0269] Table 24 Determination results of SPF and PFA of microemulsion 62-71

[0270]

[0271] Example 13 Balancing skin microbial flora, soothing skin, sunscreen, whitening cosmetics

[0272] A cosmetic with multiple functions of balancing skin microflora, soothing skin, sun protection and whitening. The cosmetic comprises the following components in mass ratio: 1% citrus fruit extract, 0.05% ceramide, 0.95% wild soybean seed extract, 1% niacinamide, 3% ethylhexyl methoxycinnamate, 2% diethylhexyl butyramido triazone, 2% diethylamino hydroxybenzoyl hexyl benzoate, 3% butylene glycol, 6% glycerin, 0.2% xanthan gum, 0.4% ammonium acryloyldimethyltaurate / VP copolymer, 5% dibutyl adipate, 5% cetyl ethylhexanoate, 2% polyglyceryl-10 stearate, 1% polysorbate-60, 0.3% sodium stearoyl lactylate, 2% cetearyl alcohol, 0.8% phenoxyethanol, and 64.3% water.

[0273] The preparation method is as follows:

[0274] S1. Add deionized water, butylene glycol, glycerol, xanthan gum, and ammonium acryloyldimethyltaurate / VP copolymer into an emulsifying pot, stir and heat to 85-88°C, stir until the materials are mixed and dispersed evenly, keep warm, and set aside;

[0275] S2. Add ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone, diethylamino hydroxybenzoyl hexyl benzoate, dibutyl adipate, and cetyl ethylhexanoate into the oil phase pot, stir and heat to 88-92°C, stir until the material is mixed and dispersed evenly; cool to 85°C, add polyglycerol-10 stearate, polysorbate-60, sodium stearoyl lactylate, and cetearyl alcohol, stir evenly, and obtain an oil phase material;

[0276] S3. Slowly add the mixed oil phase material into the emulsifier, and then homogenize at 3000r / min for 5-10 minutes until the material is evenly mixed and dispersed;

[0277] S4. Cool down to 45°C, add phenoxyethanol, niacinamide and microemulsion 5, stir evenly and continue to cool;

[0278] S5. Cool down to 38℃, take samples and filter out the material with a 500-mesh filter after passing the test.

[0279] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A composition having the effects of balancing skin microbial flora, soothing and repairing the skin, and enhancing sunscreen and whitening effects, characterized in that: The components include citrus fruit extract, ceramide and wild soybean seed extract in a mass ratio of (1-3): (0.01-0.08): (0.95-1).

2. A composition having the effects of balancing skin microbial flora, soothing, repairing skin and whitening, characterized in that: The components include citrus fruit extract, ceramide, wild soybean seed extract and niacinamide in a mass ratio of (1-3): (0.01-0.08): (0.95-1): (0.5-1.5).

3. A composition having the effects of balancing skin microbial flora, soothing, repairing skin and sun protection, characterized in that: The components include citrus fruit extract, ceramide, soybean seed extract, ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone, and diethylaminohydroxybenzoyl hexyl benzoate in a mass ratio of (1-3): (0.01-0.08): (0.95-1): (2.5-3.5): (1.5-2.5): (1.5-2.5).

4. A composition having the effects of balancing skin microbial flora, soothing, repairing skin, sun protection and whitening, characterized in that: The components include citrus fruit extract, ceramide, wild soybean seed extract, niacinamide, ethylhexyl methoxycinnamate, diethylhexyl butyramido triazone, and diethylaminohydroxybenzoyl hexyl benzoate in a mass ratio of (1-3): (0.01-0.08): (0.95-1): (0.5-1.5): (2.5-3.5): (1.5-2.5): (1.5-2.5).

5. A microemulsion that balances skin microflora, soothes and repairs the skin, and enhances sunscreen and whitening effects, characterized in that: Made from the composition described in any one of claims 1 to 4.

6. Use of the composition according to any one of claims 1 to 4 or the microemulsion according to claim 5 in balancing skin flora, soothing skin, repairing skin barrier, sun protection and / or whitening, or in preparing a product for balancing skin flora, soothing skin, repairing skin barrier, sun protection and / or whitening.

7. Use of the composition according to any one of claims 1 to 4 or the microemulsion according to claim 5 in reducing the skin irritation of chemical preservatives, fragrance allergens or niacinamide, or in the preparation of a product that reduces the skin irritation of chemical preservatives, fragrance allergens or niacinamide.

8. Use of the composition according to any one of claims 1 to 4 or the microemulsion according to claim 5 in improving the whitening effect of niacinamide, or in preparing a niacinamide whitening enhancer.

9. A cosmetic that balances skin microbial flora, soothes skin, protects against sunburn, and whitens skin, characterized in that: Contains the composition according to any one of claims 1 to 4.

10. The cosmetic according to claim 9, characterized in that: Also contains butylene glycol, glycerin, xanthan gum, ammonium acryloyldimethyltaurate / VP copolymer, dibutyl adipate, cetyl ethylhexanoate, polyglyceryl-10 stearate, polysorbate-60, sodium stearoyl lactylate, cetearyl alcohol, and phenoxyethanol.

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