Brightening and whitening composite mask and preparation method thereof

Abstract

The invention provides a brightening and whitening composite mask and a preparation method thereof. The brightening and whitening composite mask is prepared from the following raw materials in parts by weight: 30 to 40 parts of radix cynanchi atrati extract liquid, 10 to 15 parts of mussel extract, 3 to 5 parts of superoxide dismutase, 5 to 8 parts of compound amino protein liquid, 3 to 6 parts of xanthan gum, 2 to 5 parts of humectant and 0.5 to 1 part of glycosylglycerol. The cynanchum atratum leach liquor is soaked with a hypertonic solution containing proline, natural vitamin E and sodium chloride and leached with an ethanol solution, and mussels are inoculated with mycotic spores and then fermented and subjected to cold-pressing leaching. The mask prepared from the raw materials by the method is mild and stable in physicochemical property, can effectively improve the brightness of skin, and has a whitening effect.

Description

Technical Field

[0001] The invention relates to the field of hair care daily chemical products, and in particular to a whitening composite facial mask and a preparation method thereof. Background Art

[0002] Facial pigmentation can lead to skin problems such as yellow complexion, darkening of skin color, and spots. First of all, melanin is one of the causes of darkening of skin color and spots. Melanin is a protein that originally exists in the basal layer of the skin. Due to ultraviolet radiation, melanin will move layer by layer through cell metabolism to the epidermis, causing skin problems such as darkening of skin color and spots. Secondly, human aging and physical decline can lead to pigmentation. Because the clearance rate of oxygen free radicals (ROS) in the human body slows down, collagen and elastin are destroyed, and the skin loses its ability to moisturize and maintain elasticity, which will accelerate the precipitation of melanin. Therefore, external factors and aging will cause the body and skin tissue to produce free radicals that exceed the body's normal ability to clear free radicals, thereby causing the skin tissue to darken, darken, and produce spots.

[0003] There are the following problems with commercially available moisturizing and whitening products. First, the use of synthetic chemicals has side effects and rebound effects in moisturizing and whitening, and there is a problem of high prices. Secondly, moisturizing and whitening products with a single ingredient have poor effects and have the disadvantage of a long service life. Thirdly, different plant extracts are often prone to mutual inhibition of whitening effects after superposition, so how to better compound a plant composition with moisturizing and whitening effects has application value. Therefore, in order to solve the above problems, the present application provides a composition for moisturizing and whitening and a preparation method thereof. Summary of the invention

[0004] In view of this, the present invention develops a whitening composite facial mask and a preparation method thereof to solve the above problems.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A whitening composite facial mask comprises the following raw materials in parts by weight: 30-40 parts of white osmanthus extract, 10-15 parts of mussel extract, 3-5 parts of superoxide dismutase, 5-8 parts of composite amino protein liquid, 3-6 parts of xanthan gum, 2-5 parts of moisturizer and 0.5-1 part of glycerol glucoside.

[0007] The preparation method of the white violet extract is as follows:

[0008] S1. Soaking the white osmanthus in a hypertonic solution to obtain a white osmanthus soaking solution;

[0009] S2, extracting and refluxing the soaked solution of Pleurotus eryngii to obtain a Pleurotus eryngii extract;

[0010] S3, concentrating the extract of the white mulberry into an extract, diluting, centrifuging, filtering, and taking the upper layer of volatile oil to obtain a finished product;

[0011] Furthermore, the hypertonic solution comprises proline, natural vitamin E, sodium chloride and water in a mass ratio of 20-30:5-7:4-6:50-60, and the soaking time is 30-40 minutes.

[0012] Furthermore, the extraction and reflux are performed using an ethanol solution with a mass concentration of 40-50%, and the extraction is performed reflux extraction 2-3 times, each time for 0.5-1h; S3, the concentration is concentrated under reduced pressure at 55-60°C to an extract with a specific gravity of 1.10-1.14g / mL; the dilution is diluted by adding water with a volume 2-3 times that of the extract.

[0013] The preparation method of mussel extract comprises the following steps:

[0014] S1, inoculating mold spores into chopped mussels and fermenting them to obtain mussel fermentation products;

[0015] S2, cold pressing the fermented mussels and rotating at high speed to obtain mussel slurry;

[0016] S3. Filter the mussel slurry through a sieve, add sodium chloride solution, centrifuge, filter again, take the upper layer, dry, and obtain the mussel extract.

[0017] Furthermore, the fermentation temperature is 28-32°C and the fermentation time is 2-3 days.

[0018] Furthermore, the mold is Trichoderma reesei and Aspergillus oryzae in a mass ratio of 0.5-0.8:1-1.2, and the mold spore inoculation amount is 1-4×10 6 The preparation method of the spore liquid is as follows: Trichoderma reesei spores and Aspergillus oryzae spores are inoculated into PDA culture medium respectively, the temperature is 28-30°C, the pH value is 5.0-5.5, the constant temperature culture time is 2.5-3.5d, water is added and stirred to obtain a spore suspension.

[0019] Furthermore, S2, the cold pressing temperature is 40-45°C, the rotation speed is 90-110 rpm; the filtration is performed using a 100-120 mesh filter, the mass fraction of the sodium chloride solution is 0.9-1.0%, the centrifugation temperature is 3-6°C, and the time is 1-1.5h; the drying is performed using anhydrous sodium sulfate drying at a temperature of 4-6°C, and after drying, it is stored at -20 to -15°C for 6-7d.

[0020] Furthermore, the moisturizing agent is one of sodium hyaluronate, trehalose, and glycerol, and the composite amino protein liquid is collagen tripeptide and arginine in a mass ratio of (1-2): (2-2.5).

[0021] A method for preparing a whitening composite facial mask, characterized in that it comprises the following steps:

[0022] S1. Mix the white osmanthus extract, the mussel extract and the superoxide dismutase, stir and homogenize to obtain a mixture I; mix the composite amino protein solution, the moisturizer, the xanthan gum and the glycerol glucoside, stir and homogenize to obtain a mixture II;

[0023] S2. Mix the mixture I and the mixture II, homogenize them, pour them into the packaging bag containing the facial mask cloth, inspect, package and put them into storage.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The present invention uses a combination of a white osmotic extract, a mussel extract, superoxide dismutase, a composite amino protein solution, etc., and the prepared facial mask has stable physical and chemical properties. The white osmotic extract is soaked in a hypertonic solution containing proline, natural vitamin E, sodium chloride and water, extracted with an ethanol solution, and fermented and cold-pressed after the mussel is inoculated with mold spores. The facial mask prepared by this preparation method has a whitening beauty effect. Experiments show that after the facial mask prepared by the raw materials and methods is continuously applied for 45 days, the facial melanin content is significantly reduced and the melanin removal rate is significantly improved. The facial mask prepared by the raw materials and methods of the present invention is mild in nature, has no irritation reaction to the skin, does not have allergic phenomena, improves the tyrosinase inhibition rate, inhibits the generation of skin melanin, and has a good whitening effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 : Melanin particle diagram in in vitro melanin epidermal model test, the left figure shows the distribution of melanin particles in the test of the liquid prepared by the method of Example 3, and the right figure shows the distribution of melanin particles using the control 2 mask.

[0027] Figure 2 : Photo of the subjects' facial redness improvement

[0028] Figure 3 :Pictures of subjects' pigmentation improvement DETAILED DESCRIPTION

[0029] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention. The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.

[0030] Unless otherwise specified, the materials and reagents used in the examples of the present invention can be obtained from commercial sources. Trichoderma reesei: effective viable count ≥ 10 × 10 9 cfu / g, manufacturer: Guangzhou Weiyuan Technology Co., Ltd.; Aspergillus oryzae: effective viable count ≥ 2×10 9The cfu / g manufacturer is Guangzhou Weiyuan Technology Co., Ltd.

[0031] Tyrosinase inhibition rate (%) = (tyrosinase activity on the 0th day - tyrosinase activity on the 28th day) / tyrosinase activity on the 0th day × 100%

[0032] Skin gloss improvement rate (%) = (glossiness on the 28th day - glossiness on the 0th day) / glossiness on the 28th day × 100%

[0033] Preparation Example 1-1

[0034] The preparation steps of the white violet extract include:

[0035] Step (1): soak the white osmotic spring in a hypertonic solution, wherein the hypertonic solution comprises proline, natural vitamin E, sodium chloride and water in a mass ratio of 20:7:6, and the soaking time is 30 minutes to obtain a white osmotic spring soaking solution.

[0036] Step (2): extract and reflux the soaked solution of Pleurotus eryngii, wherein the extracting solution is 40% ethanol solution, and reflux extraction is performed 2-3 times, each time for 30 minutes, to obtain the Pleurotus eryngii extract.

[0037] Step (3): Concentrate the Bletilla striata extract into an extract at a concentration temperature of 55° C. The extract has a specific gravity of 1.13 g / mL, add 2 times the volume of water to dilute, centrifuge, filter, and take the upper layer of volatile oil to obtain the Bletilla striata extract.

[0038] Preparation Example 1-2

[0039] The preparation steps of the white violet extract include:

[0040] Step (1): soak the white osmotic spring in a hypertonic solution, wherein the hypertonic solution comprises proline, natural vitamin E, sodium chloride and water in a mass ratio of 30:7:4, and the soaking time is 40 minutes to obtain a white osmotic spring soaking solution.

[0041] Step (2): extract and reflux the soaked solution of Pleurotus eryngii, wherein the extract is a 50% ethanol solution, and reflux and extract for 3 times, each time for 1 hour, to obtain a Pleurotus eryngii extract.

[0042] Step (3): Concentrate the Pleurotus eryngii extract into an extract at a concentration temperature of 55° C. The specific gravity of the extract is 1.13 g / mL. Add 3 times the volume of water to dilute the extract, centrifuge, filter, and take the upper layer of volatile oil to obtain the Pleurotus eryngii extract.

[0043] Preparation Example 1-3

[0044] The preparation steps of the white violet extract include:

[0045] Step (1): soak the white osmotic spring in a hypertonic solution, wherein the hypertonic solution comprises proline, natural vitamin E, sodium chloride and water in a mass ratio of 25:6:5:50, and the soaking time is 35 minutes to obtain a white osmotic spring soaking solution.

[0046] Step (2): extracting and refluxing the soaked solution of Pleurotus eryngii, using an ethanol solution with a mass concentration of 50%, and reflux extraction 2-3 times, each time for 50 minutes, to obtain a Pleurotus eryngii extract.

[0047] Step (3): Concentrate the Pleurotus eryngii extract into an extract at a concentration temperature of 55° C. The specific gravity of the extract is 1.13 g / mL. Add 2.5 times the volume of water to dilute the extract, centrifuge, filter, and take the upper layer of volatile oil to obtain the Pleurotus eryngii extract.

[0048] Preparation Example 2-1

[0049] The preparation method of mussel extract comprises the following steps:

[0050] Step (1): crush fresh mussels and inoculate them with fungal spores. The fungi are Trichoderma reesei and Aspergillus oryzae in a mass ratio of 0.5:1. The inoculation amount of fungal spores is 1×10 6 The preparation method of the spore liquid is as follows: Trichoderma reesei spores and Aspergillus oryzae spores are inoculated into PDA plate culture medium respectively, the culture temperature is 28°C, the pH value is 5.0, and after constant temperature culture for 2.5 days, water is added and stirred to obtain a spore suspension. The spore suspension is fermented at a fermentation temperature of 28°C and a fermentation time of 2 days to obtain a mussel fermentation product;

[0051] Step (2): cold pressing the fermented mussel at a temperature of 40° C., rotating twice at a high speed of 90 revolutions per minute, to obtain mussel slurry;

[0052] Step (3): Filter the mussel slurry with a 100-mesh filter, add a 0.9% sodium chloride solution by mass, centrifuge at 3° C. for 1 hour, filter again, take the upper layer, dry at 4° C., and after drying, store at -20° C. for 6 days to obtain the mussel extract.

[0053] Preparation Example 2-2

[0054] The preparation method of mussel extract comprises the following steps:

[0055] Step (1): crush fresh mussels into particles with a particle size of 2 mm, and then inoculate with mold spores, the molds are Trichoderma reesei and Aspergillus oryzae with a mass ratio of 0.8:1.2, and the inoculation amount of mold spores is 4×10 6The preparation method of the spore liquid is as follows: Trichoderma reesei spores and Aspergillus oryzae spores are inoculated into PDA plate culture medium respectively, the culture temperature is 30°C, the pH value is 5.5, after constant temperature culture for 2.5 days, water is added and stirred to obtain a spore suspension. The spore suspension is fermented at a fermentation temperature of 32°C and a fermentation time of 3 days to obtain a mussel fermentation product;

[0056] Step (2): cold pressing the fermented mussel at a temperature of 45° C., rotating at high speed twice at a rotation speed of 110 rpm to obtain mussel slurry;

[0057] Step (3): Filter the mussel slurry with a 120-mesh filter, add a 1.0% sodium chloride solution by mass, centrifuge at 6° C. for 1.5 hours, filter again, take the upper layer, dry at 6° C., and after drying, store at -15° C. for 7 days to obtain the mussel extract.

[0058] Preparation Example 2-3

[0059] The preparation method of mussel extract comprises the following steps:

[0060] Step (1): crush fresh mussels into particles with a particle size of 2 mm, and then inoculate with mold spores, the molds are Trichoderma reesei and Aspergillus oryzae with a mass ratio of 0.7:1.1, and the inoculation amount of mold spores is 3×10 6 The preparation method of the spore liquid is as follows: Trichoderma reesei spores and Aspergillus oryzae spores are inoculated into PDA plate culture medium respectively, the culture temperature is 30°C, the pH value is 5.0, and after constant temperature culture for 3 days, water is added and stirred to obtain a spore suspension. The spore suspension is fermented at a fermentation temperature of 30°C and a fermentation time of 3 days to obtain a mussel fermentation product;

[0061] Step (2): cold pressing the fermented mussel at a temperature of 45° C., rotating at high speed twice at a rotation speed of 100 rpm to obtain mussel slurry;

[0062] Step (3): Filter the mussel slurry with a 100-mesh filter, add a 0.9% sodium chloride solution by mass, centrifuge at 5° C. for 1 hour, filter again, take the upper layer, dry at 5° C., and after drying, store at -15° C. for 6 days to obtain the mussel extract.

[0063] Example 1

[0064] The preparation of a whitening composite facial mask comprises the following steps:

[0065] Step (1): 30 parts of the white osmanthus extract prepared in Preparation Example 1-1, 10 parts of the mussel extract prepared in Preparation Example 2-1 and 3 parts of superoxide dismutase are stirred, mixed and homogenized to obtain a mixture I; 7 parts of a composite amino protein solution (the mass ratio of collagen tripeptide to arginine is 1:2.5), 4 parts of glycerol, 3 parts of xanthan gum and 0.3 parts of glycerol glucoside are stirred, mixed and homogenized to obtain a mixture II;

[0066] Step (2): Mix the mixture I and the mixture II, homogenize them, pour them into the packaging bag containing the mask cloth, inspect, package and store them.

[0067] Example 2

[0068] The preparation of a whitening composite facial mask comprises the following steps:

[0069] Step (1): 40 parts of the white osmanthus extract prepared in Preparation Example 1-2, 10-15 parts of the mussel extract prepared in Preparation Example 2-2 and 5 parts of superoxide dismutase are stirred, mixed and homogenized to obtain a mixture I; 8 parts of a composite amino protein solution (the mass ratio of collagen tripeptide to arginine is 2:2.5), 5 parts of trehalose, 6 parts of xanthan gum and 1 part of glycerol glucoside are stirred, mixed and homogenized to obtain a mixture II;

[0070] Step (2): Mix the mixture I and the mixture II, homogenize them, pour them into the packaging bag containing the mask cloth, inspect, package and store them.

[0071] Example 3

[0072] The preparation of a whitening composite facial mask comprises the following steps:

[0073] Step (1): 35 parts of the white osmanthus extract prepared in Preparation Example 1-3, 12 parts of the mussel extract prepared in Preparation Example 2-3 and 4 parts of superoxide dismutase are mixed, stirred and homogenized to obtain a mixture I; 6 parts of a composite amino protein solution (the mass ratio of collagen tripeptide to arginine is 1.5:2), 3 parts of sodium hyaluronate, 5 parts of xanthan gum and 1 part of glycerol glucoside are mixed, stirred and homogenized to obtain a mixture II;

[0074] Step (2): Mix the mixture I and the mixture II, homogenize them, pour them into the packaging bag containing the mask cloth, inspect, package and store them.

[0075] Comparative Example 1

[0076] The difference from Example 3 is that in the steps of preparing the white mulberry extract of Example 1-3, step (1) of soaking the white mulberry in a hypertonic solution is removed, and ethanol is directly used for extraction. Other raw materials and preparation steps are the same as those of Example 3.

[0077] Comparative Example 2

[0078] The difference from Example 3 is that in the steps of preparing the mussel extract in Example 2-3, the step (1) of inoculating mold spores for fermentation is removed, and the mussel extract is chopped and then cold pressed for extraction. The other raw materials and preparation steps are the same as those in Example 3.

[0079] Comparative Example 3

[0080] The difference from Example 3 is that in the steps of preparing the mussel extract of Example 2-3, the cold pressing extraction in step (2) is replaced by high temperature extraction at 80-90° C. The other raw materials and preparation steps are the same as those in Example 3.

[0081] Comparative Example 4

[0082] The difference from Example 3 is that the mass fraction of the composite amino protein liquid in the raw materials for preparing the whitening composite mask is reduced from 6 parts to 1 part, and the component superoxide dismutase is removed. The other raw materials and preparation steps are the same as those in Example 3.

[0083] Test 1:

[0084] Control 1: Kojic acid, purity greater than 98%, CAS number: 501-30-4. Control 2: A commercially available whitening mask, manufactured by Guangzhou Danzi Co., Ltd.

[0085] Experimental principle: Skinovo-Mela is an epidermal model containing melanocytes reconstructed in vitro by 3D printing with human keratinocytes and melanocytes as seed cells. The structure of this model is highly similar to human skin, with melanin particle distribution and stratified epidermal structure, and can be used for testing in the fields of cosmetic whitening and sun protection. Using the epidermal model containing melanocytes Skinovo-Mela, by testing relevant indicators, evaluate whether the sample to be tested has the corresponding efficacy. Experimental method: CALT / TM / SOP287-01 Standard operating procedures for in vitro skin model efficacy evaluation test. Experimental materials: Saifu 3D printed human skin model, culture conditions: 37℃, 5% CO2, relative humidity 95%; culture medium: human in vitro reconstructed melanin model culture medium (Lot: 20231204). Experimental steps: Skin preparation; add the pre-overflowed rice system liquid to the well plugging culture plate (m) and transfer the epidermal nucleus containing melanocytes to the 6-well plate with culture medium, and put it into the incubator for overnight culture. Sampling: Take 25 test samples and evenly apply them on the model surface. Test groups and exposure conditions, set 3 parallels for each test group. After reaching the specified exposure time, rinse the samples thoroughly with DPBS until there is no residue, and then place them in a six-well plate containing fresh maintenance culture medium for further incubation. After reaching the specified incubation time, use tweezers to remove the model from the well plate, place it in the center of the color card and take a photo for apparent color comparison, use a puncher to punch the skin tissue, and then use a sharp blade to cut the tissue. Used for value determination and melanin particle distribution determination experiments. Use Skin-Colormerer"cL400 to detect tissue L value. Melanin staining: The removed experimental tissue was fixed in 4% paraformaldehyde solution, then fully dehydrated with 30% sucrose solution, and finally wrapped with OCT frozen section wrapping agent. During the fixation process, it is necessary to remove the bubbles in the OCT glue, and then quickly placed in liquid nitrogen, marked the group and transferred to -80℃ for storage. After the tissue section is stained with a melanin staining kit. * indicates a significant difference of 5%.

[0086] Table 1

[0087]

[0088]

[0089] The test results show that the mask ingredients prepared in Examples 1-3 effectively inhibit melanin particles. Under the test conditions, the efficacy evaluation test results on the epidermal model containing melanocytes show that the mask ingredients prepared in Examples 1-3 have the effects of increasing the L value, improving the melanin deposition on the surface of the model and reducing the content of melanin particles on the epidermal model containing melanocytes, indicating that the mask ingredients prepared in Examples 1-3 have whitening effects. Test 2:

[0090] Selection of test volunteers: 40 volunteers aged 35-50 were selected, and the facial application method was adopted for the test. They were randomly divided into 8 groups, with 5 in each group. The facial masks prepared by the embodiments, comparative examples and control examples were used, and one piece was applied each night for 20-25 minutes. A total of 28 days were used, and the amount of melanin before and after applying the mask was detected on the 0th, 14th and 28th days to judge the skin purification effect. The skin moisture content test method refers to GHS-SOP-G-101, the skin hemoglobin test method refers to GHS-SOP-G-120, and the spot area ratio test method refers to GHS-SOP-G-105. The mask used in control 2 is the same as that in test 1.

[0091] The test results show that after continuous application of the facial mask prepared by the method of Examples 1-3 for 45 days, the melanin content on the face is significantly reduced, and the removal rate ranges from 45.0% to 46.9%, which is higher than that of Comparative Examples 1-4 and the control, indicating that the facial mask prepared by the method of the present invention has a good whitening effect, and changing the raw material formula or ratio will reduce the whitening effect.

[0092] Table 2

[0093]

[0094] Table 3

[0095]

[0096] The tyrosinase of the above-mentioned volunteers was measured using a tyrosinase activity detection kit (Beijing Solebow Technology Co., Ltd.). The skin gloss was measured using a skin detector (Visia skin detector). The survey statistics were used to determine whether there was skin irritation and allergy, and the survey results were recorded in Table 4. The survey results of the volunteers showed that the facial mask prepared by the raw materials and methods of the present invention was mild in nature, did not cause irritation to the skin, and did not cause allergies. The tyrosinase inhibition rate was calculated and it was found that Examples 1-3 significantly improved the tyrosinase inhibition rate, indicating that the factors affecting the production of melanin in human skin were suppressed.

[0097] Table 4

[0098]

[0099] A satisfaction questionnaire survey was conducted on 40 volunteers using the facial mask prepared by the method of Example 3, and the survey results are shown in Table 5. The survey results show that the facial mask prepared by the method of the present invention achieved good trial effects for 14 days and 28 days, and the skin had no irritation, tightness, burning sensation or itching after use.

[0100] Table 5

[0101]

[0102]

[0103] The test results are summarized. The present invention uses a combination of white osmotic extract, mussel extract, superoxide dismutase, composite amino protein liquid, moisturizer and glycerol glucoside. The mask obtained by this raw material combination is mild in nature, does not have irritation to the skin, and does not have allergic phenomena. The white osmotic extract is soaked in a hypertonic solution containing proline, natural vitamin E, sodium chloride and water, extracted with an ethanol solution, and fermented and cold-pressed after the mussel is inoculated with mold spores. This preparation method increases the content of effective ingredients in white osmotic extract, and the mask prepared by this preparation method has a whitening beauty effect. The test shows that after the facial mask prepared by the raw materials and methods of the present invention is continuously applied for 28 days, the tyrosinase inhibition rate is improved, the facial melanin content is significantly reduced, and the removal rate is significantly improved. After the raw material combination and preparation method of Comparative Examples 1-4 were changed, the whitening effect decreased. Therefore, the facial mask prepared by the raw materials and method of the present invention is mild in nature, makes the skin soft and smooth after use, has a refreshing and non-sticky texture, soothes and brightens the skin, is easily absorbed, can inhibit the activity of melanocytes, block the transport of melanocytes, promote metabolism, and inhibit the production of melanin in the skin, and has the effect of brightening and whitening the skin.

[0104] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A whitening composite facial mask, characterized in that: The invention comprises the following raw materials in parts by weight: 30-40 parts of white osmanthus extract, 10-15 parts of mussel extract, 3-5 parts of superoxide dismutase, 5-8 parts of composite amino protein liquid, 3-6 parts of xanthan gum, 2-5 parts of moisturizing agent and 0.5-1 part of glycerol glucoside.

2. A brightening composite facial mask according to claim 1, characterized in that The preparation method of the white violet extract is as follows: S1. Soaking the white osmanthus in a hypertonic solution to obtain a white osmanthus soaking solution; S2, extracting and refluxing the soaked solution of Pleurotus eryngii to obtain a Pleurotus eryngii extract; S3. Concentrate the extract of the white mulberry into an extract, dilute, centrifuge, filter, and take the upper layer of volatile oil to obtain a finished product.

3. A brightening composite facial mask according to claim 2, characterized in that: S1, the hypertonic solution comprises proline, natural vitamin E, sodium chloride and water in a mass ratio of 20-30:5-7:4-6:50-60, and the soaking time is 30-40 minutes.

4. A brightening composite facial mask according to claim 2, characterized in that: S2, the extraction and reflux, the extracting liquid is 40-50% ethanol solution, reflux extraction 2-3 times, each time 0.5-1h; S3, the concentration, under reduced pressure concentration at 55-60°C to an extract with a specific gravity of 1.10-1.14g / mL; the dilution, adding 2-3 times the volume of water of the extract for dilution.

5. The brightening composite facial mask according to claim 1, characterized in that: The preparation method of the mussel extract comprises the following steps: S1, inoculating mold spores into chopped mussels and fermenting them to obtain mussel fermentation products; S2, cold pressing the fermented mussels and rotating at high speed to obtain mussel slurry; S3. Filter the mussel slurry through a sieve, add sodium chloride solution, centrifuge, filter again, take the upper layer, dry, and obtain the mussel extract.

6. A whitening composite facial mask according to claim 5, characterized in that: The fermentation temperature is 28-32°C and the fermentation time is 2-3 days.

7. A whitening composite facial mask according to claim 5, characterized in that: S1, the mold is Trichoderma reesei and Aspergillus oryzae in a mass ratio of 0.5-0.8:1-1.2, and the mold spore inoculation amount is 1-4×10 6 The preparation method of the spore liquid is as follows: Trichoderma reesei spores and Aspergillus oryzae spores are inoculated into PDA culture medium respectively, the temperature is 28-30°C, the pH value is 5.0-5.5, the constant temperature culture time is 2.5-3.5d, water is added and stirred to obtain a spore suspension.

8. The brightening composite facial mask according to claim 5, characterized in that: S2, the cold pressing temperature is 40-45°C, the rotation speed is 90-110 rpm; the filtration is performed using a 100-120 mesh filter, the mass fraction of the sodium chloride solution is 0.9-1.0%, the centrifugation temperature is 3-6°C, and the time is 1-1.5h; the drying is performed using anhydrous sodium sulfate drying at a temperature of 4-6°C, and after drying, it is stored at -20 to -15°C for 6-7d.

9. The brightening composite facial mask according to claim 1, characterized in that: The moisturizing agent is one of sodium hyaluronate, trehalose and glycerol, and the composite amino protein liquid is collagen tripeptide and arginine in a mass ratio of (1-2): (2-2.5).

10. A method for preparing a brightening composite facial mask, characterized in that: The production steps include: S1. Mix the white osmanthus extract, the mussel extract and the superoxide dismutase, stir and homogenize to obtain a mixture I; mix the composite amino protein solution, the moisturizer, the xanthan gum and the glycerol glucoside, stir and homogenize to obtain a mixture II; S2. Mix the mixture I and the mixture II, homogenize them, pour them into the packaging bag containing the facial mask cloth, inspect, package and put them into storage.

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