Anti-inflammatory and acne-removing composition for conditioning acne-sensitive skin and application of anti-inflammatory and acne-removing composition

Abstract

The invention relates to the technical field of skin care products, and particularly discloses an anti-inflammatory and acne-removing composition for conditioning acne-sensitive skin and application of the anti-inflammatory and acne-removing composition. The acne-removing composition can solve the problem of immune disorder in the acne process, can achieve the effects of rapidly adjusting inflammatory response, diminishing inflammation and removing acnes, and takes both effectiveness and mildness into account; through scientific compatibility of the components, the composition achieves a synergistic effect, has an obvious oil control effect on a human body, is efficient and stable, and has an effect of improving the overall health degree of skin. In addition, the anti-inflammatory and acne-removing composition provided by the invention is acid-free and sulfur-free, purely natural in extraction and non-irritant, and has the characteristics of high efficiency, mildness and safety; the composition is simple and convenient to use, quick to absorb, free of residue, free of influence on daily life and free of burden on acne, oily, sensitive and other skin.

Description

Technical Field

[0001] The present application relates to the technical field of skin care products, and in particular to an anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin and its application. Background Art

[0002] Acne is a common chronic inflammatory skin disease of the hair follicles and sebaceous glands, mainly occurring in seborrheic areas such as the face, chest, and back. The course of acne is chronic and recurrent, and may lead to infection, pigmentation, and scarring. It can even have a serious negative impact on the patient's quality of life and psychosocial function.

[0003] The pathogenesis of acne is mainly related to increased sebum secretion, excessive keratinization of hair follicles and sebaceous duct epithelium, Propionibacterium acnes and other microorganisms and inflammatory immunity. At present, the effect of acne-removing products in overcoming the inflammatory cascade in acne needs to be improved. The prevention and repair of skin cell damage induced by inflammation is not comprehensive, and the skin's own immune regulation cannot be improved. The inhibitory effect on acne-removing factors is insufficient, and it is easily invaded by external factors. The skin is easily sensitive and secretes too much oil, resulting in insignificant acne-removing effects, long acne-removing cycles, and high recurrence rates in the later stages. Summary of the invention

[0004] The purpose of the present application is to overcome the deficiencies of the above-mentioned prior art and to provide an anti-inflammatory and acne-removing composition for conditioning acne-sensitive skin and its application.

[0005] To achieve the above purpose, the technical solution adopted by this application is:

[0006] The present application provides an anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin, the anti-inflammatory and anti-acne composition comprising the following components in parts by weight:

[0007] 0.001-0.1 parts of isoquercetin, 0.01-5 parts of stachyose, 0.1-8 parts of chickpea sugar alcohol, 1-18 parts of white thorn flower extract and 0.01-2 parts of oxymatrine.

[0008] The inventors of the present application have found that the present application uses isoquercetin, stachyose, chickpea sugar alcohol, white thorn flower extract and oxymatrine in a specific weight ratio to have a comprehensive effect of simultaneous acne removal, oil control and soothing. Among them, the inventors found in the actual experimental process that the selection of appropriate weight ratios of isoquercetin, white thorn flower extract and oxymatrine can play an excellent synergistic role, which can better improve skin inflammation, and the acne removal and oil control effects are more obvious. In addition, the combination of stachyose and chickpea sugar alcohol can better improve cell viability, promote the viability of keratinocytes in the epidermis and fibroblasts in the dermis, so as to improve the skin's own immune regulation and maintain the health of the skin.

[0009] Specifically, isoquercetin is a naturally occurring form of quercetin. Isoquercetin (quercetin-3-glucoside, isoqueritrin, IQ), in which a sugar replaces the hydrogen position of the original hydroxyl group at the 3rd hydroxyl group, has been shown to have better water solubility and better bioavailability than quercetin.

[0010] Stachyose is a functional oligosaccharide and a promising oligosaccharide prebiotic. It has the effects of improving gastrointestinal function, protecting the liver, enhancing immunity, delaying aging, and scavenging free radicals. It is often used in the field of food and health care products because of its weak sweetness, strong stability, and low energy value.

[0011] Chickpea sugar alcohol is a kind of oligosaccharide found in chickpeas. It participates in immune regulation, improves the body's immunity, promotes the proliferation of intestinal probiotics, and improves the intestinal microecological environment.

[0012] Sophora davidii (Franch.) Skeels, also known as white flower thorn and wolf tooth sophora, is a plant of the genus Sophora in the leguminous family. The compounds isolated from the genus Sophora mainly include flavonoids, alkaloids, phenylpropanoids, stilbene compounds, benzofuran compounds, steroids, sugars, fatty acids, amino acids, etc. The white thorn flower extract is added to the anti-inflammatory and anti-acne composition, so that the composition has anti-inflammatory and anti-allergic effects.

[0013] Oxymatrine is a natural quinolinazine alkaloid extracted from the dried roots and fruits of Sophora flavescens. It is the major bioactive compound of Sophora flavescens and is known for its multiple anti-inflammatory, antioxidant and antiviral properties. In clinical practice, topical application of Sophora flavescens, such as creams, gels and lotions containing oxymatrine, is commonly used to treat various skin-related diseases.

[0014] As a preferred embodiment of the anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin described in the present application, the anti-inflammatory and anti-acne composition comprises the following components in parts by weight:

[0015] 0.0015-0.05 parts of isoquercetin, 0.1-4 parts of stachyose, 0.5-6 parts of chickpea sugar alcohol, 2-12 parts of white thorn flower extract and 0.1-1 parts of oxymatrine.

[0016] As a preferred embodiment of the anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin described in the present application, the anti-inflammatory and anti-acne composition comprises the following components in parts by weight:

[0017] 0.003-0.02 parts of isoquercetin, 0.5-2 parts of stachyose, 0.8-2 parts of chickpea sugar alcohol, 4-10 parts of white thorn flower extract and 0.2-0.5 parts of oxymatrine.

[0018] As a preferred embodiment of the anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin described in the present application, the anti-inflammatory and anti-acne composition comprises the following components in parts by weight:

[0019] 0.01 parts of isoquercetin, 1 parts of stachyose, 1 parts of chickpea sugar alcohol, 5 parts of white thorn flower extract, and 0.5 parts of oxymatrine.

[0020] The inventors of the present application have discovered through research that when the added proportions of components in the anti-inflammatory and anti-acne composition change, it will directly affect the product's acne removal and oil control performance; when the anti-inflammatory and anti-acne composition is added to a lotion, it will affect the lotion's acne removal and oil control effects on the skin; experiments have found that the effect of gradually increasing or decreasing the content of a component on the overall effect of the product is not linear, and when the weight proportions of the components are further selected within the preferred range of the present application, the overall effect of the product obtained is the best.

[0021] Specifically, the inventors explored the dosage of each component. If the dosage of the component is too small or the total dosage of the three is too small, the synergistic effect is not obvious. If the dosage of the component is too large or the total dosage of the three is too large, antagonism may occur, which will also lead to a decrease in the acne removal and oil control effects. This shows that the above-mentioned components have a more ideal synergistic effect when within the scope of this application.

[0022] Preferably, the method for preparing the Whitethorn Flos Extract comprises the following steps:

[0023] The white thorn flower is dried, crushed and sieved, and an extraction solvent including alcohol is added to perform ultrasonic extraction on the sieved white thorn flower powder to prepare a white thorn flower extract.

[0024] The present application adopts a specific alcohol extraction method to obtain a white thorn flower extract with more active substances, which has the effect of increasing the inhibition of inflammation (inflammatory factor IL-8), and can also promote the activity of skin cells, thereby enhancing the acne removal and oil control effects. However, the white thorn flower extract adopts other methods (such as extraction method), and the active component and content obtained are not as good as the alcohol extraction method.

[0025] Preferably, the extraction solvent further comprises ethyl acetate and / or petroleum ether.

[0026] In some specific embodiments, the alcohol extraction solvent includes ethanol solution or n-butanol with a volume fraction of 70-80%.

[0027] Preferably, the alcohol extraction solvent is an ethanol solution with a volume fraction of 75%.

[0028] Preferably, the solid-to-liquid ratio of the white thorn flower powder and the extraction solvent is 1 g: (8-45) mL;

[0029] The temperature of the ultrasonic extraction is 60-90° C., the time of the ultrasonic extraction is 20-30 min, and the power of the ultrasonic extraction is 100-250 w.

[0030] In some specific embodiments, the sieving is sieving through a 60-mesh sieve.

[0031] The present application also provides the use of the anti-inflammatory and acne-removing composition for conditioning acne-sensitive skin in the preparation of cosmetics.

[0032] As a preferred embodiment of the application described in the present application, the cosmetics include one of lotion, emulsion, cream, mask, essence and spray.

[0033] The cosmetics of the present application also include dosage forms commonly used in the art.

[0034] The present application also provides a cosmetic, which includes the above-mentioned anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin, and the mass percentage of the anti-inflammatory and anti-acne composition in the cosmetic is 0.1-3%.

[0035] As a preferred embodiment of the cosmetics described in the present application, the cosmetics are emulsions, and the emulsions further include the following components in percentage by mass:

[0036] Thickener 0.05-0.5%, moisturizer 3-20%, emulsifier 0.5-10%, preservative 0.2-5% and pH adjuster 0.05-0.1%.

[0037] The anti-inflammatory and anti-acne composition is applied to cosmetics (such as lotions) to solve the problem of immune disorder in the process of acne, and can achieve the effect of quickly regulating inflammatory response, reducing redness and inflammation, and taking into account both effectiveness and mildness. In addition, through the scientific combination of components, it can achieve a synergistic effect, has obvious acne removal and oil control effects on human body, and can also promote the vitality of keratinocytes in the epidermis and fibroblasts in the dermis, improving the overall health of the skin.

[0038] As a preferred embodiment of the cosmetics described in the present application, the thickener includes at least one of xanthan gum, carbomer 980, ammonium acryloyldimethyltaurate / VP copolymer and polymer cellulose;

[0039] The moisturizing agent includes at least one of glycerin, butylene glycol and 30kDa sodium hyaluronate;

[0040] The emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate and polymethylsilsesquioxane;

[0041] The preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone;

[0042] The pH regulator includes arginine and / or disodium ethylenediaminetetraacetate.

[0043] The present application also provides a method for preparing the above-mentioned cosmetic, comprising the following steps:

[0044] (1) Mixing a humectant, a thickener, a pH adjuster and water, heating to 80° C., homogenizing at a speed of 1200 rpm for 5 minutes, and keeping the mixture warm for later use to obtain a prefabricated component A;

[0045] (2) After heating the emulsifier to 80° C., homogenize at a speed of 1200 rpm for 5 minutes. After the homogenization is completed, keep the temperature for later use to obtain the prefabricated B component;

[0046] (3) heating the preservative to 60° C. and melting it to obtain a prefabricated C component;

[0047] (4) The preformed component A is heated to 80°C, and the preformed component B is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to 60°C, and the preformed component C is added at a speed of 300 rpm and stirred and mixed. Then, the temperature is lowered to below 45°C, and the anti-inflammatory and anti-acne composition is added and stirred for 5 minutes. Then, the pH is adjusted to 5.0-6.5 with a pH adjuster, and stirring is stopped. The material is discharged to obtain an emulsion.

[0048] Compared with the prior art, this application has the following beneficial effects:

[0049] The present application provides an anti-inflammatory and acne-removing composition for conditioning acne-sensitive skin and its application. The acne-removing composition of the present application can solve the problem of immune disorder in the process of acne, and can achieve the effect of rapid regulation of inflammatory response, anti-inflammatory and acne-removing, taking into account effectiveness and mildness; secondly, through scientific compatibility components, it can achieve a synergistic effect, has obvious oil control effect in human efficacy, takes into account high efficiency and stability, and has the effect of improving the overall health of the skin. In addition, the anti-inflammatory and acne-removing composition provided by the present application is acid-free and sulfur-free, purely naturally extracted, non-irritating, and has the characteristics of high efficiency, mildness and safety. It is simple and convenient to use, absorbs quickly, has no residue, does not affect daily life, and is burden-free for acne, oily, sensitive and other skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] Figure 1 This is a graph showing the acne removal test results of using the emulsion of Example 11 on days D0 and D28 in Effect Example 3;

[0051] Figure 2 This is a graph showing the acne removal test results using the emulsion of Comparative Example 19 on days D0 and D28 in Effect Example 3. DETAILED DESCRIPTION

[0052] In order to better illustrate the purpose, technical solutions and advantages of the present application, the present application will be further described below in conjunction with specific embodiments.

[0053] In the present application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0054] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0055] In the following examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified, and the components and raw materials used in each parallel experiment are of the same type.

[0056] In the following examples and comparative examples:

[0057] Isoquercetin: HPLC ≥ 98%, purchased from Sichuan Jingcui Tiancheng Pharmaceutical Technology Co., Ltd.;

[0058] Stachyose: HPLC ≥ 98%, purchased from Shanghai Chenqiao Biotechnology Co., Ltd.;

[0059] Chickpea sugar alcohol: HPLC ≥ 98%, purchased from Sichuan Jingcui Tiancheng Pharmaceutical Technology Co., Ltd.;

[0060] Oxymatrine: HPLC ≥ 98%, purchased from Sichuan Jingcui Tiancheng Pharmaceutical Technology Co., Ltd.;

[0061] Hyperoside: HPLC ≥ 98%, Shanghai Bio-Tech Biotechnology Co., Ltd.;

[0062] Fructo-oligosaccharide: CAS: 308066-66-2, Shanghai MacLean Biochemical Technology Co., Ltd.;

[0063] Lupeol: CAS: 545-47-1, Shanghai Yien Chemical Technology Co., Ltd.;

[0064] Astragalus extract: Product number: S27211, Shanghai Yuanye Biotechnology Co., Ltd.;

[0065] Oxysophorine: CAS: 26904-64-3, Shanghai Aladdin Biochemical Technology Co., Ltd.

[0066] The present application provides a method for preparing Whitethorn Flower Extracts 1-4;

[0067] The preparation method of the Whitethorn Flower Extract 1 of the present application comprises the following steps:

[0068] 1) After the collected white thorn flower is cleaned, it is dried in an oven at 45° C., taken out, and crushed with a grinder and passed through a 60-mesh sieve to obtain white thorn flower powder for later use;

[0069] 2) Weigh the obtained White Thorn Flower powder and place it in a 100 mL conical flask, add 75% ethanol by volume, the solid-liquid ratio of the White Thorn Flower powder and ethanol is 1 g:10 mL, soak for 45 minutes, and perform ultrasonic extraction for 20 minutes. The temperature of the ultrasonic extraction is 80°C, the power of the ultrasonic extraction is 200 w, and the process is repeated three times. The ethanol is removed, and the extracts are combined to obtain White Thorn Flower extract 1.

[0070] The preparation method of the Whitethorn Flower Extract 2 of the present application comprises the following steps:

[0071] 1) After the collected white thorn flower is cleaned, it is dried in an oven at 45° C., taken out, and crushed with a grinder and passed through a 60-mesh sieve to obtain white thorn flower powder for later use;

[0072] 2) Weigh the obtained White Thorn Flower powder and place it in a 100mL conical flask, add ethyl acetate, the solid-liquid ratio of the White Thorn Flower powder and ethyl acetate is 1g:10mL, ultrasonic extraction for 30min, the temperature of ultrasonic extraction is 80℃, the power of ultrasonic extraction is 200w, remove fat, evaporate the solvent, add 70% methanol by volume at 40mL / g, soak for 45min, ultrasonic extraction for 20min, the temperature of ultrasonic extraction is 80℃, the power of ultrasonic extraction is 200w, repeat three times, combine the extracts, and then centrifuge at 4500r / min for 15min, discard the precipitate, obtain White Thorn Flower extract 2, put it in a 50mL volumetric flask, and make up to volume.

[0073] The preparation method of the Whitethorn Flower Extract 3 of the present application is the same as the preparation method of the Whitethorn Flower Extract 2, the only difference being that petroleum ether is used instead of ethyl acetate to prepare the Whitethorn Flower Extract 3.

[0074] The preparation method of the Whitethorn Flower Extract 4 of the present application is the same as the preparation method of the Whitethorn Flower Extract 2, the only difference being that n-butanol is used instead of ethyl acetate to prepare the Whitethorn Flower Extract 4.

[0075] According to the different polarities of the components contained in the white thorn flower, the present application uses solvents of different polarities to extract and separate the components of different polarity segments. White thorn flower extract 1 is the total substance extracted by alcohol, and white thorn flower extracts 2-4 are respectively extracted by ethyl acetate, petroleum ether, and n-butanol. The active components and contents are different, and the effects produced are also different.

[0076] Embodiment 1-10, an anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin

[0077] The present embodiments 1-10 provide an anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin, comprising the following components:

[0078] Isooquercetin, stachyose, chickpea sugar alcohol, white thorn flower extract 1-4 (any one) and oxymatrine.

[0079] The specific formulas (parts by weight) of Examples 1-10 are shown in Table 1.

[0080] The preparation method of the anti-inflammatory and acne-removing composition for conditioning acne-sensitive skin is as follows: weigh the corresponding mass parts of isoquercetin and dissolve it in 4 mL of 1,3-pentanediol, and weigh the corresponding mass parts of stachyose, chickpea sugar alcohol, white thorn flower extract, and oxymatrine and dissolve them in ultrapure water, and mix the five substances evenly at room temperature and an ultrasonic power of 100 w.

[0081] Table 1

[0082]

[0083] Comparative Example 1-18: An anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin

[0084] Comparative Examples 1-18 provide an anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin, and the specific formula is shown in Table 2. The preparation method of the anti-inflammatory and anti-acne composition provided in Comparative Examples 1-18 is similar to that of Examples 1-10.

[0085] Table 2

[0086]

[0087]

[0088]

[0089] Examples 11-20 and Comparative Examples 19-36, an anti-acne lotion

[0090] This embodiment provides an acne-removing lotion. The anti-inflammatory and acne-removing compositions prepared in Examples 1-10 and Comparative Examples 1-18 are respectively applied to the acne-removing lotions to prepare the acne-removing lotions of Examples 11-20 and Comparative Examples 19-36.

[0091] The specific formulas of the anti-acne lotions of Examples 11-20 and Comparative Examples 19-36 are shown in Table 3 (mass percentage).

[0092] The preparation method of the anti-acne lotion is as follows:

[0093] (1) Mix phase A and water, heat to 80° C., and homogenize at 1200 rpm for 5 min. After homogenization, keep the mixture warm for later use to obtain prefabricated phase A;

[0094] (2) Mix phase B, heat to 80° C., and homogenize at 1200 rpm for 5 min. After homogenization, keep warm for later use to obtain prefabricated phase B;

[0095] (3) Mix phase C and heat to 60°C to melt to obtain prefabricated phase C;

[0096] (4) The preformed phase A is heated to 80°C, and the preformed phase B is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to 60°C, and the preformed phase C is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to below 45°C, and the anti-inflammatory and anti-acne composition and essence are added, and stirring is continued for 5 minutes. Finally, arginine is added to adjust the pH to 5.0-6.5, and stirring can be stopped. The material is discharged to obtain an emulsion.

[0097] The only difference between the anti-acne lotions of Examples 11-20 and Comparative Examples 19-36 is the different anti-inflammatory and anti-acne compositions, and the other components and component mass percentages are the same, and the preparation methods are also the same.

[0098] Table 3

[0099]

[0100]

[0101] Example 21, an anti-acne lotion

[0102] Example 21 provides an anti-acne lotion, and the specific formula is shown in Table 4. The preparation method of the anti-acne lotion of Example 21 is the same as that of Examples 11-20.

[0103] Table 4

[0104]

[0105] Comparative Example 37, an emulsion

[0106] Compared with the anti-acne emulsion of Examples 11-20, the anti-inflammatory and anti-acne composition is not contained, and the other components and the mass percentages of the components are the same, and the preparation method is also the same.

[0107] Effect Example 1: Inflammatory Factor Test

[0108] This effect example explores the anti-inflammatory effects of the acne-removing compositions (test samples) prepared in Examples 1-10 and Comparative Examples 1-18.

[0109] 1. Experimental materials:

[0110] 1.1 Cell line: HaCaT cells, source: Guangzhou Customs Technology Center, passage number: 14.

[0111] 1.2 Culture medium: MEM culture medium containing 10% fetal bovine serum (FBS, Gibco, Lot: 2500251P).

[0112] 1.3 Test conditions: incubator temperature 37±1℃, humidity 90±5%, CO2 5±1%.

[0113] 1.4 Experimental controls and reagents:

[0114] 1.4.1 Experimental groups are shown in Table 5:

[0115] Table 5 Experimental groups

[0116]

[0117] 1.5 Experimental reagents:

[0118] MTT solution: Prepare MTT into a 5 mg / mL MTT storage solution using PBS, filter and sterilize using a 0.22 μM syringe filter, and store at 20°C.

[0119] Peptidoglycan: Dilute to working concentration with ultrapure water, batch number: 5924-43-01

[0120] IL-8 kit: H220527-008a.

[0121] 2. Test steps:

[0122] 2.1 For routine cell culture, inoculate the cell suspension into a 12-well plate and return to the incubator for 18-24 hours.

[0123] 2.2 Take out the culture plate, discard the original culture medium in the well, add 1 mL of culture medium containing 0.15% test sample and 50 μg / mL peptidoglycan to each well of the sample group. Add 50 μg / mL peptidoglycan as a stimulus to the model group, add 1 mL of 50 μg / mL dexamethasone and 50 μg / mL peptidoglycan to the positive control, and add culture medium to the control group, and culture for 24±1h.

[0124] 2.3 Collect the supernatant and store it at -80℃. Detect cytokines using ELISA kit.

[0125] 3. Data analysis:

[0126] Data were analyzed by SPSS and expressed as mean values. If P < 0.05, the difference was considered statistically significant. The relative content of IL-8 in each group was calculated with the model control group (M) as 100%.

[0127] Table 6

[0128]

[0129]

[0130] Note: Compared with the negative control group, the significance is indicated by #, and P value < 0.01 is indicated by ##; compared with the model group, the significance is indicated by *, P value < 0.05 is indicated by *, P value < 0.01 is indicated by **, P value < 0.0001 is indicated by ****, and no significance, P value > 0.05 is indicated by ns.

[0131] Propionibacterium acnes can activate Toll-like receptors (TLR-2 and TLR-4) on keratinocytes, leading to the activation of MAPK and NF-kB pathways. Subsequently, keratinocytes produce IL-1, IL-6, IL-8, TNF-α, and human HBD-2 (defensins), etc., thereby inducing inflammation. In this experiment, peptidoglycan-induced human keratinocytes were used as an in vitro inflammatory cell model, and the inflammatory factor IL-8 secreted by the cell supernatant was detected by an ELSA kit to evaluate the anti-inflammatory effect of the sample, so as to prove that the anti-acne composition prepared in Examples 1-10 has the effect of removing acne.

[0132] The test results are shown in Table 6. The anti-acne compositions prepared in Examples 1-10 provided in the present application have an inhibitory effect on the overexpression of IL-8 induced by peptidoglycan, and the inhibitory effects are all significant. Among them, the anti-acne composition prepared in Example 1 has the best inhibitory effect.

[0133] Among them, compared with Example 1 and Examples 8-10, the alcohol extraction method can be used to obtain the Whitethorn Flower extract with more active substances, thereby improving the synergistic effect of the acne-removing composition.

[0134] However, when any one or two of isoquercetin, stachyose, chickpea sugar alcohol, white thorn flower extract, and oxymatrine were missing, the IL-8 inhibition effect of the obtained comparative examples 1-9 was significantly reduced, among which the combination of isoquercetin, white thorn flower extract and oxymatrine had a better effect on inhibiting inflammation.

[0135] Moreover, when other components with similar functions replace one of isoquercetin, stachyose, chickpea sugar alcohol, white thorn flower extract, and oxymatrine, the acne-removing composition obtained in comparative examples 10-14 has an inhibitory effect on inflammation that is far inferior to that obtained by using the technical solution of the present application, which illustrates the irreplaceability of the components in the acne-removing composition of the present application.

[0136] In the anti-acne compositions of Comparative Examples 15-18, the weight proportions of isoquercetin, stachyose, chickpea sugar alcohol, white thorn flower extract, and oxymatrine are not within the scope of the present application, and the anti-acne compositions have less effect on inhibiting inflammation than Examples 1-10.

[0137] Effect Example 2: Skin Cell Vitality Test

[0138] This effect example explores the cell viability promoting effect of the acne-removing composition prepared in Examples 1-10 and Comparative Examples 1-18.

[0139] 1. Experimental materials:

[0140] Phosphate buffered saline (PBS), Shanghai Solebold; MTT, Yuanye Biotechnology; dimethyl sulfoxide (DMSO), Tianjin Damao; high glucose medium 1×DMEM, Gibco; fetal bovine serum (FBS), Gibco; keratinocytes were purchased from Guangzhou Customs Technology Center; human skin fibroblasts HSF were purchased from Kunming Cell Bank, Chinese Academy of Sciences.

[0141] 2. Experimental steps:

[0142] Inoculate the cell suspension in a 96-well cell culture plate and return to the incubator for 18-24 hours. Discard the original culture medium in the wells, add 100 μL of 0.60% test sample to each well, return to the incubator and incubate for 24±1 hours, and use the culture medium as the blank control group. Take out the culture plate, add 20 μL of MTT solution to each well, and incubate in the incubator for 3-4 hours. Remove the liquid in the wells, add 100 μL DMSO to each well, place it on the oscillator for 10-15 minutes, and then measure the absorbance OD value at a wavelength of 570 nm on the microplate reader, and calculate the cell viability.

[0143] Cell viability = (OD value 样品孔 -OD value 调零 ) / (OD value 空白对照孔 -OD value 调零 )×100%.

[0144] Table 7

[0145]

[0146]

[0147] Note: Compared with the blank control group, the significance is indicated by *, P value < 0.05 is indicated by *, and no significance, P value > 0.05 is indicated by ns.

[0148] The test results are shown in Table 7. The acne-removing compositions prepared in Examples 1-10 provided in the present application have a promoting effect on skin cells, and their effects on promoting the proliferation of keratinocytes in the epidermis and fibroblasts in the dermis are significant, among which Example 1 has the best inhibitory effect.

[0149] Among them, the whitening and repairing composition in the present application optimizes the extraction conditions of the White Thorn Flower extract. Comparing Example 1 and Example 8-10, the alcohol extraction method can obtain the White Thorn Flower extract with more active substances, thereby improving the synergistic effect of the acne-removing composition; while the whitening and repairing composition prepared by the secondary extraction method for preparing the White Thorn Flower extract (Examples 8-10) is not as effective as Example 1.

[0150] However, when any one of isoquercetin, stachyose, chickpea sugar alcohol, white thorn flower extract, and oxymatrine is lacking, the anti-acne composition of comparative examples 1-9 obtained has a less promoting effect on skin cells than that of examples 1-10, and the activity of keratinocytes and fibroblasts is significantly reduced. Among them, the combined use of stachyose and chickpea sugar alcohol has a deeper effect on promoting the proliferation of skin cells.

[0151] Moreover, when other components with similar functions replace one of isoquercetin, stachyose, chickpea sugar alcohol, white thorn flower extract, and oxymatrine, the effects obtained in comparative examples 10-14 are far inferior to those obtained by using the technical solution of the present application. Moreover, when the above components are within the scope of the present application, they have a relatively ideal synergistic effect.

[0152] Effect Example 3: Human efficacy test: acne, papules, redness, and oil content

[0153] This effect example tests the improvement effects of the anti-acne lotions of Examples 11-21 and Comparative Examples 19-37 on acne, redness and oiliness of the human body.

[0154] The specific test method is as follows:

[0155] According to the "Technical Specifications for Safety of Cosmetics" (2015), 240 volunteers with sensitive skin aged 18-45 (lactic acid stinging score>3) were selected and randomly divided into 30 groups, 8 people in each group, and the volunteers had to have at least 2-3 obvious acne. Under normal circumstances, the volunteers continued to use the product on their entire face for 28 days; every morning and evening, after cleansing and moisturizing, the volunteers took an appropriate amount of lotion and applied it to the face, massaged it for absorption, and the acne area could be overlapped. The whole face was used once in the morning and evening, and a follow-up visit was made every day. On the day of the volunteer's visit, the test area washed its face with clean water without applying any product, and sat quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes.

[0156] The improvement of facial acne indicators was quantified and photographed using instruments, including Visia 7 (IPP) test of facial red area a*, acne count (classification count: comedones, papules, respectively evaluated by VISIA and doctors), and Sebumeter SM 815 test of oil content. Improvement rate before and after use = (X 使用前均值 -X 使用后均值 ) / X 使用前均值 ×100%; the calculation results are shown in Table 8.

[0157] Table 8

[0158]

[0159]

[0160] Note: Compared with the base material (i.e., Comparative Example 37), significance is indicated by *, P value < 0.05 is indicated by *, and no significance, P value > 0.05 is indicated by ns.

[0161] The test results are shown in Table 8. The anti-acne lotions provided in Examples 11-21 of the present application have an improvement effect on acne, and the improvement effects on acne, papules, a*, and oil are all significant, among which the lotion in Example 11 has the best effect on inhibiting acne. Figure 1 As shown, on the 28th day after the subjects used Example 11, their facial acne was significantly improved, and the volunteers reported that it was mild and non-irritating and were willing to continue using it.

[0162] Compared with Example 1 and Examples 18-20, the extraction method of the White thorn Flower extract is preferably the alcohol extraction method. The prepared White thorn Flower extract has better properties and is more conducive to being used in anti-acne lotion, thereby better playing the role of inhibiting acne.

[0163] However, when any one or two of isoquercetin, stachyose, chickpea sugar alcohol, white thorn flower extract, and oxymatrine are missing, the improvement effect of the emulsions of comparative examples 19-27 obtained is significantly reduced, and there is a certain effect of aggravating acne. Figure 2As shown, on the 28th day after the subject used Comparative Example 19, there was no improvement in facial acne, and part of the acne was ripened and became red and swollen, and the purpose of acne removal could not be achieved.

[0164] When other components with similar functions replace one of isoquercetin, stachyose, chickpea sugar alcohol, white thorn flower extract, and oxymatrine, the effects of the emulsions of comparative examples 28-32 are far inferior to those of the emulsions of examples 11-21. And when the above components are within the scope of the present application, they have a relatively ideal synergistic effect.

[0165] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present application rather than to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, ordinary technicians in this field should understand that the technical solution of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present application.

Claims

1. An anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin, characterized in that: The anti-inflammatory and anti-acne composition comprises the following components in parts by weight: 0.001-0.1 parts of isoquercetin, 0.01-5 parts of stachyose, 0.1-8 parts of chickpea sugar alcohol, 1-18 parts of white thorn flower extract and 0.01-2 parts of oxymatrine.

2. The anti-inflammatory and anti-acne composition for treating acne-sensitive skin according to claim 1, characterized in that: The anti-inflammatory and anti-acne composition comprises the following components in parts by weight: 0.0015-0.05 parts of isoquercetin, 0.1-4 parts of stachyose, 0.5-6 parts of chickpea sugar alcohol, 2-12 parts of white thorn flower extract and 0.1-1 parts of oxymatrine.

3. The anti-inflammatory and anti-acne composition for treating acne-sensitive skin according to claim 2, characterized in that: The anti-inflammatory and anti-acne composition comprises the following components in parts by weight: 0.003-0.02 parts of isoquercetin, 0.5-2 parts of stachyose, 0.8-2 parts of chickpea sugar alcohol, 4-10 parts of white thorn flower extract and 0.2-0.5 parts of oxymatrine.

4. The anti-inflammatory and anti-acne composition for treating acne-sensitive skin according to claim 3, characterized in that: The anti-inflammatory and anti-acne composition comprises the following components in parts by weight: 0.01 parts of isoquercetin, 1 parts of stachyose, 1 parts of chickpea sugar alcohol, 5 parts of white thorn flower extract, and 0.5 parts of oxymatrine.

5. The anti-inflammatory and anti-acne composition for treating acne-sensitive skin according to claim 1, characterized in that: The preparation method of the white thorn flower extract comprises the following steps: The white thorn flower is dried, crushed and sieved, and an extraction solvent including alcohol is added to perform ultrasonic extraction on the sieved white thorn flower powder to prepare a white thorn flower extract.

6. The anti-inflammatory and anti-acne composition for treating acne-sensitive skin according to claim 5, characterized in that: The extraction solvent also includes ethyl acetate and / or petroleum ether.

7. The anti-inflammatory and anti-acne composition for treating acne-sensitive skin according to claim 5, characterized in that: The solid-liquid ratio of the white thorn flower powder and the extraction solvent was 1 g: (8-45) mL; The temperature of the ultrasonic extraction is 60-90° C., the time of the ultrasonic extraction is 20-30 min, and the power of the ultrasonic extraction is 100-250 w.

8. Use of the anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin according to any one of claims 1 to 7 in the preparation of cosmetics.

9. The use according to claim 8, characterized in that The cosmetics include one of lotion, emulsion, cream, facial mask, essence and spray.

10. A cosmetic, characterized in that: The cosmetic comprises the anti-inflammatory and anti-acne composition for conditioning acne-sensitive skin according to any one of claims 1 to 7, and the mass percentage of the anti-inflammatory and anti-acne composition in the cosmetic is 0.1-3%.

11. The cosmetic according to claim 10, characterized in that The cosmetic is an emulsion, which further comprises the following components in percentage by mass: Thickener 0.05-0.5%, moisturizer 3-20%, emulsifier 0.5-10%, preservative 0.2-5% and pH adjuster 0.05-0.1%.

12. The cosmetic according to claim 11, characterized in that The thickener comprises at least one of xanthan gum, carbomer 980, ammonium acryloyldimethyltaurate / VP copolymer and high molecular weight cellulose; The moisturizing agent includes at least one of glycerin, butylene glycol and 30kDa sodium hyaluronate; The emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate and polymethylsilsesquioxane; The preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone; the pH adjuster includes arginine and / or disodium ethylenediaminetetraacetate.

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