The invention discloses a normal temperature adhesive composition, which comprises emulsion one, emulsion two, an anti-adhesion agent, a wetting agent, an antioxidant, a defoaming agent, ammonia and water, wherein the emulsion one is one of acrylosilane emulsion, pure acrylic emulsion, styrene-acrylate emulsion, vinyl acetate/acrylic emulsion and vinyl acetate-acetate copolymer emulsion; the molecular weight of the emulsion one is smaller than 100,000 and the glass state temperature thereof is between -2 and +45DEG C; the emulsion two is one of polyacrylic emulsion, SIS latex and natural latex; and the molecular weight of the emulsion two is between 300,000 and 1,000,000, and the glass state temperature thereof is between -50 and -80DEG C; and the raw materials are added one by one, and the mixture is mixed, stirred, dispersed evenly to prepare the normal temperature adhesive composition. The sealing composition finally achieves effective sealing under the condition of different sealing pressures by properly adjusting a ratio of an adhesion system to a pressure-sensitive system according to different sealing conditions.