Herbal compound-containing antibacterial eye drops for pets and preparation method of herbal compound-containing antibacterial eye drops

Abstract

The invention provides antibacterial eye drops containing a herbal compound for pets and a preparation method of the antibacterial eye drops. The antibacterial eye drops for the pets are prepared from the following components in percentage by mass and volume: 1%-5% of a compound of a honeysuckle flower extract and a dandelion extract, 0.2%-1% of hyaluronic acid, 0.01%-0.05% of vitamin B2, 0.001%-0.005% of menthol and the balance of water for injection. The antibacterial eye drops for pets not only have efficient antibacterial and anti-inflammatory effects, but also are mild and non-irritant in formula, free of toxic and side effects, suitable for pets to use for a long time and capable of maintaining eye health of pets for a long time.

Description

Technical Field

[0001] The present invention belongs to the field of pet medical preparations, and in particular relates to pet antibacterial eye drops containing a herbal compound and a preparation method thereof. Background Art

[0002] Tear staining in pets is a significant health and cosmetic concern for many pet owners, especially in specialized breeds such as Garfields, Pomeranians, and Maltese dogs. Due to inherent structural issues in the eye, excess tears can accumulate in the skin around the eyes, breeding bacteria and causing redness, swelling, and inflammation, leading to more serious eye problems. Current treatment options often involve antibiotic eye drops for eye infections. However, long-term use of these drops can lead to bacterial resistance, reducing their effectiveness and making them ineffective for long-term protection.

[0003] Therefore, it is particularly important to develop a safe and effective pet antibacterial eye drops for daily protection and treatment. Summary of the Invention

[0004] In response to the problems existing in the prior art, the purpose of the present invention is to provide a pet antibacterial eye drops containing a herbal compound and a preparation method thereof. The pet antibacterial eye drops not only have high antibacterial and anti-inflammatory effects, but also have a mild and non-irritating formula and no toxic side effects. They are suitable for long-term use by pets and can maintain the pet's eye health in the long term.

[0005] Natural herbal extracts have been shown to have antibacterial and anti-inflammatory properties without side effects, but their application in treating bacterial infections in pet eyes remains underdeveloped. By combining and formulating multiple active ingredients, more effective sterilization and inhibition of tear stain-causing bacteria can be achieved, reducing the risk of infection. Furthermore, the soothing and anti-inflammatory properties of herbal ingredients are crucial for alleviating eye discomfort and effectively maintaining eye health. Regular use of such products can actively promote normal tear secretion and drainage, significantly reducing tear accumulation. Furthermore, the combination of moisturizing ingredients such as hyaluronic acid can precisely maintain the moisture balance of the eye area, which plays a significant role in reducing the appearance of tear stains. With the increasing awareness of pet grooming and health, the market demand for safe and effective tear stain care products is growing. Against this backdrop, the development of antibacterial eye drops based on natural herbal ingredients is urgent, and herbal antibacterial properties are a key component of this research and development, providing important support and assurance for meeting market demand and resolving the tear stain problem in pets. With this in mind, the inventors have provided the following solution.

[0006] The purpose of the present invention is achieved through the following technical solutions: The first aspect of the present invention provides a pet antibacterial eye drop containing a herbal compound, wherein the pet antibacterial eye drop comprises the following components in the following mass volume percentages: A mixture of honeysuckle extract and dandelion extract 1%-5%, hyaluronic acid 0.2%-1%, vitamin B2 0.01%-0.05%, menthol 0.001%-0.005%, and the balance is water for injection.

[0007] In some specific embodiments, the pet antibacterial eye drops contain the following components in the following mass volume percentages: 2% mixture of honeysuckle extract and dandelion extract, 0.3% hyaluronic acid, 0.02% vitamin B2, 0.002% menthol, and the balance is water for injection; or 3% mixture of honeysuckle extract and dandelion extract, 0.4% hyaluronic acid, 0.03% vitamin B2, 0.003% menthol, and the balance is water for injection.

[0008] Furthermore, the honeysuckle extract and dandelion extract compound is a 1:1 compound of honeysuckle extract and dandelion extract.

[0009] The second aspect of the present invention provides a method for preparing the pet antibacterial eye drops containing the herbal compound described in the first part above, comprising the following steps: (1) Weigh hyaluronic acid and add it to water for injection. Stir in a water bath to prepare solution A. (2) Weigh the honeysuckle extract and dandelion extract, mix them, dissolve them in water for injection, and stir them evenly to obtain solution B; (3) Weigh vitamin B2 and menthol, dissolve them in propylene glycol, and stir evenly to obtain solution C; (4) adding solution B and solution C to solution A to obtain a semi-finished eye drop solution (wherein, solution B and solution C are added in no particular order); (5) Filtering the semi-finished eye drops product, and aseptically filling it to obtain the pet antibacterial eye drops containing the herbal compound.

[0010] Furthermore, in step (1), the dissolution temperature of hyaluronic acid is 50°C-60°C.

[0011] Furthermore, in step (5), the filtration is performed using an organic filter membrane having a pore size of 0.22 μM.

[0012] The third aspect of the present invention provides the use of the pet antibacterial eye drops containing the herbal compound described in the first aspect in the preparation of products for treating bacterial infections of pet eyes.

[0013] The beneficial effects of the present invention compared to the prior art are: 1. Honeysuckle extract has anti-inflammatory properties that can reduce eye inflammation and discomfort; secondly, the natural antibacterial ingredients can inhibit bacterial growth and prevent eye infections. Honeysuckle extract can also relieve eye fatigue and irritation, provide comfort, and the rich antioxidant ingredients help protect pets' eye tissues from free radical damage and promote eye health. In addition, it may support the eye's self-repair ability by improving local immunity. These properties make honeysuckle extract a valuable ingredient suitable for use in eye care products; 2. Dandelion extract is rich in antioxidants, which help protect eye tissues, resist free radical damage, and promote eye health. In addition, dandelion extract has anti-inflammatory properties, which can reduce eye discomfort and inflammation. At the same time, its natural antibacterial effect can help prevent eye infections and increase product safety. Dandelion extract also has a soothing effect, which can relieve eye fatigue and improve user comfort. These advantages make dandelion extract an ideal ingredient in pet eye care products; 3. The combination of honeysuckle extract and dandelion extract has shown significant beneficial effects and innovations in pet eye drops. The 1:1 combination of the two not only achieves a more excellent antibacterial effect, but the antibacterial properties of honeysuckle can effectively relieve eye discomfort and prevent infection, while the antioxidant and soothing effects of dandelion help protect eye tissue and relieve fatigue. This synergistic effect not only improves the overall efficacy of the product, but also enhances the safety and comfort of pet eye care. Importantly, the compounded extract is a pure Chinese herbal ingredient with no side effects and will not cause harm to the pet's eyes. It is more in line with the needs of modern pet owners for natural health products, thereby providing pets with more comprehensive eye protection; 4. Hyaluronic acid has multiple beneficial effects in the pet antibacterial eye drops containing the herbal compound described in this application. First, it is a powerful moisturizer that can effectively lock in moisture and keep the eyes moist, thereby alleviating dry eye symptoms and discomfort. At the same time, hyaluronic acid has good adhesion and can form a protective film to help stabilize tears and reduce eye irritation. It also has anti-inflammatory properties, which helps to reduce eye inflammation and redness and promote eye health. These advantages of hyaluronic acid make it an important ingredient in pet eye care products, providing comfortable and safe eye protection for pets; 5. Adding a small amount of menthol to eye drops can quickly reduce eye temperature and relieve redness, swelling, and discomfort caused by inflammation or allergies. This immediate soothing effect makes pets feel noticeably more comfortable after use, reducing scratching or frequent blinking. 6. Vitamin B2 (riboflavin), as an important water-soluble vitamin, plays an important role in enhancing ocular cell regeneration and repair. It not only helps reduce eye redness and discomfort caused by infection, promotes wound healing, and improves eye health, but also effectively relieves irritation and discomfort caused by excessive tearing. It also improves the composition of tears, making them easier to drain and reducing the formation of tear stains. Proper use of vitamin B2 not only improves your pet's eye health but can also be used as an eyewash for daily protection against bacterial eye infections, providing pet owners with a safe and effective care option. 7. Furthermore, experiments have confirmed that the pet antibacterial eye drops containing the herbal compound provided by the present invention have a highly effective bactericidal effect on Staphylococcus aureus and Escherichia coli, with survival rates of less than 0.1%, indicating that the herbal extract compound has an excellent bactericidal effect and can be used for bacterial infections in pet eyes, providing a new strategy for solving the current antibiotic crisis. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments: Figure 1 Schematic diagram of the bactericidal effect of herbal extracts; among them, (A) schematic diagram of the bactericidal effect of honeysuckle extract on Staphylococcus aureus; (B) schematic diagram of the bactericidal effect of dandelion extract on Staphylococcus aureus; (C) schematic diagram of the bactericidal effect of honeysuckle extract on Escherichia coli; (D) schematic diagram of the bactericidal effect of dandelion extract on Escherichia coli; (E) schematic diagram of the synergistic bactericidal effect of herbal extracts on Staphylococcus aureus; (F) synergistic bactericidal effect of herbal extracts on Escherichia coli.

[0015] Figure 2 Schematic diagram of the bactericidal effect of herbal extracts with different compound ratios; (A) Schematic diagram of the bactericidal effect of herbal extracts with different compound ratios on Staphylococcus aureus; (B) Schematic diagram of the bactericidal effect of herbal extracts with different compound ratios on Escherichia coli.

[0016] Figure 3 Schematic diagram of the bactericidal effect of Example 1 on Staphylococcus aureus and Escherichia coli. DETAILED DESCRIPTION

[0017] The examples are provided to better illustrate the present invention, but are not intended to limit the present invention to the examples. Therefore, non-essential improvements and adjustments to the embodiments made by those skilled in the art based on the above-mentioned invention still fall within the scope of protection of the present invention.

[0018] The endpoints of the ranges and any values ​​disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoints of each range, the endpoints of each range and individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered to be specifically disclosed herein.

[0019] The present invention will be described in detail below through examples. It should be understood that the following examples are only used to further explain and illustrate the content of the present invention in detail, and are not intended to limit the present invention.

[0020] The raw materials involved in the embodiment of the present invention include: Dandelion extract, hyaluronic acid, vitamin B2, menthol, and propylene glycol were all purchased from Shanghai MacLean Biochemical Technology Co., Ltd., and honeysuckle extract was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0021] The following embodiments of the present invention relate to detection methods including: 1. The antibacterial activity in the following examples was determined using the broth microdilution method. Bacterial cells (Staphylococcus aureus and Escherichia coli) were collected by centrifugation and then resuspended in saline solution. The cell suspension (10 8 CFU / mL) were added to the treated cells and incubated at 37°C, 200 rpm. After incubation, bacterial cells (Staphylococcus aureus and Escherichia coli) were harvested by centrifugation, suspended in an equal volume of saline solution, and serially diluted 10-fold. Finally, six dilutions from each condition were plated on LB agar and incubated overnight at 37°C. Colony forming units (CFU) were counted and the inhibition rate was calculated.

[0022] Inhibition rate (%) = (1-CFU sample / CFU control) × 100% 2. The antibacterial effect assay in the following examples was performed using the spread plate method. Monoclonal bacterial cells were cultured overnight in LB medium. Bacterial cells (Staphylococcus aureus and Escherichia coli) were harvested by centrifugation and then resuspended in physiological saline solution. The cell suspension (10 8 CFU / mL) and incubate at 37°C, 200 rpm. After incubation, bacterial cells (Staphylococcus aureus and Escherichia coli) were collected by centrifugation and suspended in an equal volume of saline solution. 10 μL of the bacteria was evenly spread on an agar plate and cultured in an incubator overnight for observation. Example 1

[0023] This embodiment provides a pet antibacterial eye drop containing a herbal compound, wherein the pet antibacterial eye drop is composed of the following components in the following mass volume percentages: 2% honeysuckle extract and dandelion extract compound, 0.3% hyaluronic acid, 0.02% vitamin B2, 0.002% menthol, and the balance is water for injection. The honeysuckle extract and dandelion extract compound is a 1:1 compound of honeysuckle extract and dandelion extract.

[0024] The preparation method of the pet antibacterial eye drops comprises the following steps: (1) Weigh hyaluronic acid and add it to water for injection. Stir in a 50°C water bath to prepare solution A. (2) Weigh the honeysuckle extract and dandelion extract, mix them, dissolve them in water for injection, and stir them evenly to obtain solution B; (3) Weigh vitamin B2 and menthol, dissolve them in propylene glycol, and stir evenly to obtain solution C; (4) adding solution B and solution C to solution A to obtain a semi-finished eye drop solution (wherein, solution B and solution C are added in no particular order); (5) The semi-finished eye drops are filtered through a 0.22 μM organic filter membrane and aseptically filled to obtain the pet antibacterial eye drops containing the herbal compound. Example 2

[0025] This embodiment provides a pet antibacterial eye drop containing a herbal compound, which differs from Example 1 in that in step (1) of the preparation method, the water bath temperature is set to 55°C. Example 3

[0026] This embodiment provides a pet antibacterial eye drop containing a herbal compound, wherein the pet antibacterial eye drop contains the following components in the following mass volume percentages: 3% honeysuckle extract and dandelion extract compound, 0.4% hyaluronic acid, 0.03% vitamin B2, 0.003% menthol, and the balance is water for injection. The honeysuckle extract and dandelion extract compound is a 1:1 compound of honeysuckle extract and dandelion extract.

[0027] The preparation was carried out according to the preparation method described in Example 1, except that: in step (1), the temperature of the water bath was set to 60°C. Test Case

[0028] This test case is to verify the antibacterial activity of the herbal extract compound against bacteria. The following tests are performed: 1. Prepare two herbal extract solutions of honeysuckle extract and dandelion extract respectively, and dilute them in a gradient manner; treat the cells with drugs diluted to different concentrations for 10 8CFU / mL of Staphylococcus aureus and Escherichia coli, and the total bacterial colony count was determined by gradient drop plate experiment after 3 h to explore the antibacterial activity of two herbal extract solutions of honeysuckle extract and dandelion extract.

[0029] The herbal extracts are grouped as follows: (1) Honeysuckle extract; (2) Dandelion extract; (3) A combination of honeysuckle extract and dandelion extract (1:1).

[0030] The antibacterial activities of the above herbal extracts against Staphylococcus aureus and Escherichia coli were evaluated by broth dilution method.

[0031] result: Figure 1 The antibacterial effects of honeysuckle extract, dandelion extract and their 1:1 mixture on bacteria were demonstrated. Figure 1 As shown in Figure 2, the minimum inhibitory concentration (MIC) of honeysuckle extract against Staphylococcus aureus was 0.32% ( Figure 1 The MIC of honeysuckle extract against Escherichia coli was 0.64% ( Figure 1 The MIC of dandelion extract against Staphylococcus aureus is 0.64% ( Figure 1 The MIC of dandelion extract against Escherichia coli was 1.28% ( Figure 1 The combined antibacterial effect of honeysuckle extract and dandelion extract is shown in Figure 1 The results showed that the combined use of honeysuckle extract and dandelion extract reduced the minimum inhibitory concentration of Staphylococcus aureus from 0.64% to 0.32% ( Figure 1 The minimum inhibitory concentration of honeysuckle extract and dandelion extract against Escherichia coli was reduced from 1.28% to 0.64% ( Figure 1 Middle F).

[0032] 2. Mixtures of honeysuckle extract and dandelion extract were prepared at different ratios (1:1, 1:2, and 2:1, respectively; final concentration was 1%). The antibacterial activity of these herbal extracts against Staphylococcus aureus and Escherichia coli was evaluated using the broth dilution method.

[0033] result: Figure 2 The results showed that the 1:1 mixture had an antibacterial rate of over 99% against both Staphylococcus aureus and Escherichia coli. The 1:2 mixture had an antibacterial rate of 80% against Staphylococcus aureus and 78% against Escherichia coli. The 2:1 mixture had an antibacterial rate of 92% against Staphylococcus aureus and 96% against Escherichia coli. The results showed that a 1:1 ratio of honeysuckle extract to dandelion extract had the best antibacterial effect among the different mixture ratios.

[0034] 3. The antibacterial activity of Example 1 was investigated using the spread plate method, with untreated bacteria serving as a blank control.

[0035] result: Figure 3 The coating results show that the pet antibacterial eye drops prepared in Example 1 have good antibacterial effects on Staphylococcus aureus and Escherichia coli.

[0036] In summary, the combination of honeysuckle extract and dandelion extract reduces the inhibitory dosage of each, especially when the two are mixed in a 1:1 ratio, a better antibacterial effect is achieved, and its application prospects are broader.

[0037] Finally, it should be noted that the above is only used to illustrate the technical solution of the present invention and is not limiting. Although the present invention is described in detail with reference to the preferred arrangement scheme, ordinary technicians in this field should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A pet antibacterial eye drops containing a herbal compound, characterized in that: The pet antibacterial eye drops contain the following components in percentage by mass and volume: A mixture of honeysuckle extract and dandelion extract 1%-5%, hyaluronic acid 0.2%-1%, vitamin B2 0.01%-0.05%, menthol 0.001%-0.005%, and the remainder is water for injection.

2. The pet antibacterial eye drops according to claim 1, characterized in that: The pet antibacterial eye drops contain the following components in percentage by mass and volume: 2% mixture of honeysuckle extract and dandelion extract, 0.3% hyaluronic acid, 0.02% vitamin B2, 0.002% menthol, and the balance is water for injection.

3. The pet antibacterial eye drops according to claim 1, characterized in that: The pet antibacterial eye drops contain the following components in percentage by mass and volume: 3% mixture of honeysuckle extract and dandelion extract, 0.4% hyaluronic acid, 0.03% vitamin B2, 0.003% menthol, and the balance is water for injection.

4. The pet antibacterial eye drops according to any one of claims 1 to 3, characterized in that: The honeysuckle extract and dandelion extract compound is a 1:1 compound of the honeysuckle extract and the dandelion extract.

5. A method for preparing the pet antibacterial eye drops containing the herbal compound as claimed in any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: (1) Weigh hyaluronic acid and add it to water for injection. Stir in a water bath to prepare solution A. (2) Weigh the honeysuckle extract and the dandelion extract, mix them and dissolve them in water for injection, and stir them evenly to obtain solution B; (3) Weigh vitamin B2 and menthol, dissolve them in propylene glycol, and stir evenly to obtain solution C; (4) adding solution B and solution C to solution A to obtain a semi-finished eye drop solution; (5) Filtering the semi-finished eye drops, and aseptically filling them to obtain the pet antibacterial eye drops containing the herbal compound.

6. The preparation method according to claim 5, characterized in that: In step (1), the dissolution temperature of hyaluronic acid is 50°C-60°C.

7. The preparation method according to claim 5, characterized in that: In step (5), the filtration is performed using an organic filter membrane.

8. Use of the pet antibacterial eye drops containing the herbal compound as claimed in any one of claims 1 to 4 in the preparation of products for bacterial infection of pet eyes.

Images