Traditional Chinese medicine composition for tonifying deficiency and preparation process thereof

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a deficiency-tonifying traditional Chinese medicine composition and a preparation process thereof. According to the invention, codonopsis pilosula, morinda officinalis, honey-fried licorice root, fructus piperis longi, roasted rhizoma atractylodis macrocephalae, semen cuscutae, fructus alpiniae oxyphyllae, peach kernel and fructus lycii are used as raw materials and are decocted, bromelain and neutral protease are used for enzymolysis, saccharomyces cerevisiae numbered as CICC 30241 and bacillus subtillis numbered as CICC 22062 are used for fermentation, ethanol is added for extraction, filtration, ultrafiltration and other processes are carried out, and the health-care food is obtained. The composition with the excellent deficiency tonifying effect is obtained, and the composition further has the good immunity improving effect.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a deficiency-tonifying traditional Chinese medicine composition and a preparation process thereof. Background Art

[0002] Deficiency syndrome refers to the symptoms of insufficient body energy, which weakens the body's ability to resist disease and reduces physiological functions. Symptoms include pale face and lips, fatigue, palpitations and shortness of breath, spontaneous sweating and night sweats, loose stools, frequent urination, tender tongue without coating, and weak pulse.

[0003] Existing deficiency-tonifying products include Guizhi Decoction, Guifu Dihuang Pills, Fuzi Lizhong Pills, and Buzhong Yiqi Decoction, but the drug composition is biased and the deficiency-tonifying effect is not good; the dosage is large, which easily exceeds the tolerance of qi and blood, but the deficiency-tonifying effect is not good. Therefore, providing a Chinese medicine composition with a mild medicinal property and suitable for people with weak constitutions to tonify deficiency is a technical problem that needs to be solved at present. Summary of the invention

[0004] The present invention aims at the above-mentioned technical problems and proposes a technical solution of the present invention, which includes a deficiency-tonifying Chinese medicine composition and a preparation process thereof.

[0005] The invention provides a deficiency-tonifying Chinese medicine composition, and its preparation raw materials include codonopsis pilosula, morinda officinalis, roasted liquorice root, longan, stir-fried atractylodes macrocephala, dodder seed, alkyl oxyphylla seed, peach kernel and wolfberry fruit.

[0006] Furthermore, the prepared raw materials include 0.1-1 parts of Codonopsis pilosula, 0.3-2 parts of Morinda officinalis, 0.1-1.2 parts of roasted Licorice root, 0.2-1.8 parts of Piper longum, 0.1-1 parts of stir-fried Atractylodes macrocephala, 0.1-0.8 parts of Cuscuta australis, 0.1-0.7 parts of Alpinia oxyphylla, 0.2-2 parts of Prunus persica and 0.2-2.5 parts of Lycium barbarum.

[0007] The present invention also provides a method for preparing a deficiency-tonifying Chinese medicine composition, comprising the following steps:

[0008] Step I: according to weight parts, 0.1-1 parts of Codonopsis pilosula, 0.3-2 parts of Morinda officinalis, 0.1-1.2 parts of Radix Glycyrrhizae Preparata, 0.2-1.8 parts of Piper longum, 0.1-1 parts of Rhizoma Atractylodis Macrocephalae, 0.1-0.8 parts of Cuscuta australis, 0.1-0.7 parts of Alpinia oxyphyllae, 0.2-2 parts of Semen persicae and 0.2-2.5 parts of Fructus Lycii are crushed to obtain a mixed powder, and the mixture is decocted with water and cooled to obtain an intermediate material;

[0009] Step II: adding 0.5-3% bromelain and 0.4-2.5% neutral protease to the intermediate material, compared with the weight of the intermediate material, enzymolysis, enzyme inactivation, and obtaining an enzymolysis material;

[0010] Step III: adding 1.5-4% glucose and 0.01-0.05% potassium dihydrogen phosphate to the enzymatic hydrolysis material, sterilizing to obtain a sterilized mixture, inoculating fermentation bacteria consisting of brewer's yeast numbered CICC 30241 and Bacillus subtilis numbered CICC 22062 into the sterilized mixture, fermenting, sterilizing, and obtaining a fermentation material;

[0011] Step IV: adding ethanol to the fermentation material, heating and stirring to obtain a mixture;

[0012] Step V: the mixture is allowed to stand, filtered, ultrafiltered, and the ultrafiltrate is freeze-dried to obtain the deficiency-tonifying Chinese medicine composition.

[0013] Furthermore, in step I, the mass ratio of the mixed powder to water is 1:10-30.

[0014] Furthermore, the decoction temperature is 80-90° C. and the decoction time is 1-3 hours.

[0015] Further, cool to room temperature.

[0016] Furthermore, the enzymolysis temperature is 30-42°C and the time is 3-7 hours.

[0017] Furthermore, the amount of fermentation bacteria inoculated in the sterilized mixture was 2.9×10 10 -5.5×10 10 cfu / mL.

[0018] Furthermore, the ratio of the number of live bacteria of Saccharomyces cerevisiae numbered CICC 30241 and Bacillus subtilis numbered CICC22062 in the fermentation bacteria is 0.2-0.5:1.

[0019] Furthermore, the fermentation temperature is 30-40°C and the fermentation time is 70-120 hours.

[0020] Furthermore, the mass of ethanol added is 0.25-0.45 times the mass of water added in step I.

[0021] Furthermore, the heating and stirring temperature is 40-60° C., and the time is 3-6 hours.

[0022] Furthermore, the standing temperature is room temperature, and the standing time is 0.5-2 hours.

[0023] Furthermore, the filtration is performed using a filter membrane.

[0024] Furthermore, the ultrafiltration is performed using an ultrafiltration membrane with a molecular weight cut-off lower than 1500 Da.

[0025] Further, freeze-drying is performed until the water content is less than 7.3%.

[0026] The present invention also provides the method of preparing the deficiency-tonifying Chinese medicine composition into dosage forms such as tea substitute, powder, pill, paste, etc. according to actual needs.

[0027] Beneficial effects of the present invention:

[0028] The prescription of the present invention is mild, and the peach kernel and wolfberry fruit in the present invention are milder than the cinnamon twig and white peony root in the common tonic medicine Guizhi Decoction. The codonopsis pilosula, Morinda officinalis, roasted licorice, Piper longum, stir-fried atractylodes macrocephala, Cuscuta australis, Alpinia oxyphylla, peach kernel and wolfberry fruit in the present invention are milder than the prepared rehmannia root, cornus fruit, cinnamon twig and aconite in the common tonic medicine Guifu Dihuang Pill, and are also milder than the codonopsis pilosula, roasted licorice, stir-fried atractylodes macrocephala, dried ginger and aconite in the common tonic medicine Fuzi Lizhong Pill. The overall medicinal properties of the prescription of the present invention are mild, which is particularly beneficial for tonic of weak constitution.

[0029] The medicine prepared by the present invention has mild medicinal properties and small dosage, which is beneficial to replenishing deficiency; the dosage form is flexible and can be selected in many ways, and can be designed into dosage forms such as tea substitute, powder, pill, paste, etc. according to actual needs. When used as a decoction, it can be decocted according to the conventional decoction method of traditional Chinese medicine and then taken warm. When used as a tea substitute, it is taken with boiled water, cooled to a palatable state and then taken warm. When used as a powder, all the Chinese medicine pieces in the prescription are first mixed and then powdered, and when taken, a certain dose of boiled water is brewed, and after cooling to a palatable state, it is taken together with the powder, or directly put into the mouth and dissolved. When used as a pill, it can be taken according to the traditional preparation method of the pill, and a certain dose of warm boiled water is taken when taken.

[0030] The composition prepared by the present invention can significantly improve the exhaustion time of mice and increase hemolysin A 540nm The composition of the present invention has excellent anti-fatigue and tonic effects, and can also enhance the body's immunity.

[0031] The anti-fatigue effect test of the composition prepared by the present invention on mice showed that, compared with the deficiency syndrome model group, the proportion of increased exhaustion time reached 78.19%.

[0032] The test of the composition of the present invention on improving immunity showed that compared with the blank group, it increased hemolysin A 540nm The proportion reached 55.16%.

[0033] The present invention adopts the combination of Morinda officinalis and Piper longum to enhance the anti-fatigue and immunity-enhancing effects of the composition. The combination of the two makes the active ingredients and types complement each other, giving the composition a better effect.

[0034] The present invention adopts bromelain and neutral protease to perform enzymolysis together, which can complement each other to promote the effects of effective ingredients and enhance the anti-fatigue and immunity effects of the composition.

[0035] The present invention adopts a fermentation method of specific bacteria to significantly improve the deficiency-tonifying effect of the composition. The fermentation bacteria composed of two bacteria, Saccharomyces cerevisiae numbered CICC30241 and Bacillus subtilis numbered CICC 22062, produce a synergistic effect, which mutually promotes the anti-fatigue and immunity-enhancing effects of the composition. In particular, the ratio of the number of live bacteria of the two falls within the range of 0.2-0.5:1, and the effect produced is better.

[0036] The present invention uses ethanol for further extraction, which can improve the effect of the composition, and the effect is better when the mass of ethanol added is 0.25-0.45 times the mass of water added in step I. DETAILED DESCRIPTION

[0037] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0038] Saccharomyces cerevisiae numbered CICC 30241: the strain number is CICC 30241, purchased from China Industrial Microbiological Culture Collection Administration Center. Bacillus subtilis numbered CICC 22062: the strain number is CICC 22062, purchased from China Industrial Microbiological Culture Collection Administration Center.

[0039] 1. Preparation of Composition

[0040] Composition 1:

[0041] The preparation method is as follows:

[0042] Step I: according to weight parts, 0.3 parts of Codonopsis pilosula, 1.2 parts of Morinda officinalis, 0.4 parts of roasted Licorice root, 1.1 parts of Piper longum, 0.3 parts of stir-fried Atractylodes macrocephala, 0.25 parts of Cuscuta australis, 0.3 parts of Alpinia oxyphylla, 1.2 parts of Prunus persica and 1.8 parts of Lycium barbarum are crushed to obtain a mixed powder, 20 times the weight of the mixed powder is added with water, decocted at 85° C. for 1.5 hours, cooled to room temperature, and obtained an intermediate material;

[0043] Step II: adding 1.2% bromelain (100,000 U / g) and 0.8% neutral protease (100,000 U / g) to the intermediate material, stirring at 38° C. and 60 rpm for 4.2 hours, inactivating the enzyme, and obtaining an enzymolysis material;

[0044] Step III: adding 2.5% glucose and 0.03% potassium dihydrogen phosphate in weight relative to the weight of the enzymatic hydrolysis material to the enzymatic hydrolysis material, sterilizing to obtain a sterilized mixture, inoculating the sterilized mixture with fermentation bacteria consisting of Saccharomyces cerevisiae numbered CICC 30241 and Bacillus subtilis numbered CICC 22062, fermenting at 37.5° C. for 92 hours, and sterilizing to obtain a fermentation material;

[0045] The amount of fermentation bacteria inoculated in the sterilized mixture was 4.25×10 10 cfu / mL, the ratio of the number of live bacteria of Saccharomyces cerevisiae numbered CICC30241 and Bacillus subtilis numbered CICC 22062 in the fermentation bacteria was 0.4:1;

[0046] Step IV: Add ethanol in an amount 0.3 times the mass of water in step I to the fermented material, heat and stir at 50° C. for 4.8 hours to obtain a mixture;

[0047] Step V: The mixture was allowed to stand at room temperature for 1 hour, filtered through a 0.5 μm filter membrane, and then ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 800 Da. The ultrafiltrate was freeze-dried at -42°C to a water content of 3.78% to obtain Composition 1.

[0048] Composition 2:

[0049] The preparation method is as follows:

[0050] Step I: according to weight parts, 0.4 parts of Codonopsis pilosula, 0.9 parts of Morinda officinalis, 0.35 parts of roasted Licorice root, 1.4 parts of Piper longum, 0.45 parts of stir-fried Atractylodes macrocephala, 0.2 parts of Cuscuta australis, 0.2 parts of Alpinia oxyphylla, 1.1 parts of Prunus persica and 1.85 parts of Lycium barbarum are crushed to obtain a mixed powder, 20 times the weight of the mixed powder is added with water, decocted at 85° C. for 1.5 hours, cooled to room temperature, and obtained an intermediate material;

[0051] Step II: adding 0.7% bromelain (100,000 U / g) and 1.4% neutral protease (100,000 U / g) to the intermediate material, stirring at 37° C. and 60 rpm for enzymolysis for 5.5 hours, inactivating the enzyme, and obtaining an enzymolysis material;

[0052] Step III: adding 2.6% glucose and 0.035% potassium dihydrogen phosphate in weight relative to the weight of the enzymatic hydrolysis material to the enzymatic hydrolysis material, sterilizing to obtain a sterilized mixture, inoculating the sterilized mixture with fermentation bacteria consisting of Saccharomyces cerevisiae numbered CICC 30241 and Bacillus subtilis numbered CICC 22062, fermenting at 37° C. for 87 hours, and sterilizing to obtain a fermentation material;

[0053] The amount of fermentation bacteria inoculated in the sterilized mixture was 4.3×10 10 cfu / mL, the ratio of the number of live bacteria of Saccharomyces cerevisiae numbered CICC30241 and Bacillus subtilis numbered CICC 22062 in the fermentation bacteria was 0.5:1;

[0054] Step IV: Add ethanol in an amount 0.35 times the mass of water in step I to the fermented material, heat and stir at 52° C. for 4.5 hours to obtain a mixture;

[0055] Step V: The mixture was allowed to stand at room temperature for 1 hour, filtered through a 0.5 μm filter membrane, and then ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 800 Da. The ultrafiltrate was freeze-dried at -42°C to a water content of 3.75% to obtain Composition 2.

[0056] Composition 3:

[0057] Step I is: Step I: According to mass parts, 0.3 parts of Codonopsis pilosula, 0.4 parts of roasted liquorice, 2.3 parts of Piper longum, 0.3 parts of stir-fried Atractylodes macrocephala, 0.25 parts of Cuscuta australis, 0.3 parts of Alpinia oxyphylla, 1.2 parts of peach kernel and 1.8 parts of Lycium barbarum are crushed to obtain a mixed powder, 20 times the mass of water of the mixed powder is added, decocted at 85°C for 1.5 hours, cooled to room temperature, and an intermediate material is obtained; other uniform composition 1 is the same.

[0058] Composition 4:

[0059] Step I is: according to mass parts, 0.3 parts of Codonopsis pilosula, 2.3 parts of Morinda officinalis, 0.4 parts of roasted liquorice, 0.3 parts of stir-fried Atractylodes macrocephala, 0.25 parts of Cuscuta australis, 0.3 parts of Alpinia oxyphylla, 1.2 parts of peach kernel and 1.8 parts of Lycium barbarum are crushed to obtain a mixed powder, 20 times the mass of the mixed powder is added with water, decocted at 85°C for 1.5 hours, cooled to room temperature, and an intermediate material is obtained; the other uniform composition 1 is the same.

[0060] Composition 5:

[0061] Step II is: adding 2% bromelain (100,000 U / g) to the intermediate material, stirring at 38° C. and 60 rpm for 4.2 hours, inactivating the enzyme, and obtaining an enzymolysis material; the other homogeneous composition 11 is the same.

[0062] Composition 6:

[0063] Step II: Add 2% neutral protease (100,000 U / g) to the intermediate material, stir at 38° C. and 60 rpm for 4.2 hours to perform enzymolysis, inactivate the enzyme, and obtain an enzymolysis material; other steps are the same as those of the uniform composition 1.

[0064] Composition 7:

[0065] Step III is: adding 2.5% glucose and 0.03% potassium hydrogen phosphate in weight relative to the weight of the enzymatically hydrolyzed material, sterilizing to obtain a sterilized mixture, and then placing at 37.5° C. for 92 hours, sterilizing to obtain a material; other steps are the same as those of the uniform composition 1.

[0066] Composition 8:

[0067] In step III, the amount of fermentation bacteria inoculated in the sterilized mixture is 4.25×1010 cfu / mL, the fermentation bacteria is Saccharomyces cerevisiae numbered CICC 30241; the other homogeneous composition 1 is the same.

[0068] Composition 9:

[0069] In step III, the amount of fermentation bacteria inoculated in the sterilized mixture is 4.25×10 10 cfu / mL, the fermentation bacteria is Bacillus subtilis numbered CICC 22062; other components are the same as those of uniform composition 1.

[0070] Composition 10:

[0071] In step III, the amount of fermentation bacteria inoculated in the sterilized mixture is 4.25×10 10 cfu / mL, the ratio of the number of live bacteria of Saccharomyces cerevisiae numbered CICC 30241 to that of Bacillus subtilis numbered CICC 22062 in the fermentation bacteria is 0.05:1; the other parts are the same as those of the uniform composition 1.

[0072] Composition 11:

[0073] In step III, the amount of fermentation bacteria inoculated in the sterilized mixture is 4.25×10 10 cfu / mL, the ratio of the number of live bacteria of Saccharomyces cerevisiae numbered CICC 30241 to that of Bacillus subtilis numbered CICC 22062 in the fermentation bacteria is 0.8:1; the other parts are the same as those of the uniform composition 1.

[0074] Composition 12:

[0075] Step IV is: adding ethanol with a mass that is 0.65 times the mass of water in step I to the fermented material, heating and stirring at 50° C. for 4.8 hours to obtain a mixture; other uniform composition 1 is the same.

[0076] 2. Effect Test

[0077] Anti-fatigue effect test:

[0078] A number of male Kunming mice (SPF healthy, 18-22g) were selected and fed adaptively for 3 days. Mouse thyroid tablets (from Shanghai Zhonghua Pharmaceutical Co., Ltd.) were given by gavage at 160mg / kg. The drug was diluted with saline to form a solution, and the gavage dose was 0.2mL solution / 10g mouse, once a day for 14 days, to establish an animal model of deficiency syndrome.

[0079] The deficiency syndrome animal model was divided into groups (model group and the above-mentioned composition groups 1-12, a total of 13 groups), with 10 mice in each group; in addition, 10 mice (normal mice) that were adaptively fed and not constructed into the deficiency syndrome animal model were set up as a blank control group.

[0080] The mice in the composition 1-12 groups were gavaged with the corresponding composition, and the gavage dose was 3g composition / kg mouse, which was diluted with physiological saline and gavaged at 0.3mL solution / 10g mouse; the model group and the blank control group were gavaged with an equal amount of physiological saline; a weighted swimming test was performed after 14 consecutive days of administration. Half an hour after administration on the 14th day, a weighted swimming experiment was performed. Specifically: in a swimming box with a water temperature of about 26°C, mice were loaded with steel blocks weighing about 5.5% of their body weight for weighted swimming, and then the mice were artificially driven to swim continuously until they were completely submerged in the water for about 7s without coming out of the water, which was defined as exhaustion, and the total swimming time was recorded. The results are shown in Table 1.

[0081] Table 1: Anti-fatigue effect test

[0082]

[0083] 2. Test on improving immunity

[0084] A number of male Kunming mice (SPF grade healthy, 18-22g) were selected and fed conventionally with free drinking water for a total of 4 days. Then they were randomly divided into groups, with 5 mice in each group, and the groups were blank group and composition groups 1-12 respectively. Among them, the blank control group did not receive any treatment, and they were free to eat and drink water; the mice in composition groups 1-12 were each gavaged with the corresponding composition, and the gavage dose was 3g composition / kg mouse, diluted with physiological saline and gavaged at 0.3mL solution / 10g mouse, and they were free to eat and drink water. The experiment lasted for a total of 2 weeks. Then the serotonin activity level was tested. The test results are shown in Table 2.

[0085] Table 2: Test on the effect of improving immunity

[0086]

[0087]

[0088] The test results in Table 1-2 show that the composition prepared by the present invention can significantly improve the exhaustion time of mice and increase the hemolysin A 540nm This shows that the composition of the present invention has excellent anti-fatigue and tonic effects, and can also enhance the body's immunity.

[0089] The anti-fatigue effect test of the composition prepared by the present invention on mice showed that, compared with the deficiency syndrome model group, the proportion of increased exhaustion time reached 78.19%.

[0090] The test of the composition of the present invention on improving immunity showed that compared with the blank group, it increased hemolysin A 540nm The proportion reached 55.16%.

[0091] The test results of compositions 1, 3-4 in the table show that the combination of Morinda officinalis and Piper longum in the present invention can enhance the anti-fatigue and immunity-enhancing effects of the composition. The combination of the two makes the active ingredients and types complement each other, giving the composition a better effect.

[0092] Through the test of compositions 1, 5-6 in the table, it can be known that the present invention adopts bromelain and neutral protease for enzymatic hydrolysis together to promote the effects of the effective ingredients with complementary advantages, and can enhance the anti-fatigue and immunity-enhancing effects of the composition.

[0093] It can be seen from the test of compositions 1, 7-11 in the table that the present invention adopts a fermentation method of specific bacteria to significantly improve the tonic effect of the composition. The fermentation bacteria composed of two bacteria, Saccharomyces cerevisiae numbered CICC 30241 and Bacillus subtilis numbered CICC 22062, produce a synergistic effect, which mutually promotes the anti-fatigue and immunity-enhancing effects of the composition. In particular, the ratio of the number of live bacteria between the two falls within the range of 0.2-0.5:1, and the effect produced is better.

[0094] It can be seen from the tests of compositions 1 and 12 in the table that the present invention uses ethanol for further extraction to improve the effect of the composition, and the effect is better when the mass of ethanol added is 0.25-0.45 times the mass of water added in step I.

[0095] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A Chinese medicine composition for tonifying deficiency, characterized in that: The preparation raw materials include codonopsis pilosula, morinda officinalis, roasted liquorice root, piper longum, stir-fried atractylodes macrocephala, dodder seed, alpinia oxyphylla seed, peach kernel and wolfberry fruit.

2. The Chinese medicine composition for tonifying deficiency according to claim 1, characterized in that: In terms of mass, the raw materials include 0.1-1 parts of Codonopsis pilosula, 0.3-2 parts of Morinda officinalis, 0.1-1.2 parts of Radix Glycyrrhizae, 0.2-1.8 parts of Piper longum, 0.1-1 parts of stir-fried Atractylodes macrocephala, 0.1-0.8 parts of Cuscuta australis, 0.1-0.7 parts of Alpinia oxyphylla, 0.2-2 parts of Peach kernel and 0.2-2.5 parts of Lycium barbarum.

3. A method for preparing a deficiency-tonifying Chinese medicine composition, characterized in that: The steps include: Step I: according to weight parts, 0.1-1 parts of Codonopsis pilosula, 0.3-2 parts of Morinda officinalis, 0.1-1.2 parts of Radix Glycyrrhizae Preparata, 0.2-1.8 parts of Piper longum, 0.1-1 parts of Rhizoma Atractylodis Macrocephalae, 0.1-0.8 parts of Cuscuta australis, 0.1-0.7 parts of Alpinia oxyphyllae, 0.2-2 parts of Semen persicae and 0.2-2.5 parts of Fructus Lycii are crushed to obtain a mixed powder, and the mixture is decocted with water and cooled to obtain an intermediate material; Step II: adding 0.5-3% bromelain and 0.4-2.5% neutral protease to the intermediate material, compared with the weight of the intermediate material, enzymolysis, enzyme inactivation, and obtaining an enzymolysis material; Step III: adding 1.5-4% glucose and 0.01-0.05% potassium dihydrogen phosphate to the enzymatic hydrolysis material, sterilizing to obtain a sterilized mixture, inoculating fermentation bacteria consisting of brewer's yeast numbered CICC 30241 and Bacillus subtilis numbered CICC 22062 into the sterilized mixture, fermenting, sterilizing, and obtaining a fermentation material; Step IV: adding ethanol to the fermentation material, heating and stirring to obtain a mixture; Step V: the mixture is allowed to stand, filtered, ultrafiltered, and the ultrafiltrate is freeze-dried to obtain the deficiency-tonifying Chinese medicine composition.

4. The preparation method according to claim 3, characterized in that: In step I, the mass ratio of the mixed powder to water is 1:10-30.

5. The preparation method according to claim 3, characterized in that: The decoction temperature is 80-90°C and the time is 1-3 hours.

6. The preparation method according to claim 3, characterized in that: The enzymatic hydrolysis temperature is 30-42°C and the time is 3-7 hours.

7. The preparation method according to claim 3, characterized in that: The amount of fermentation bacteria inoculated in the sterilized mixture was 2.9×10 10 -5.5×10 10 cfu / mL.

8. The preparation method according to claim 3, characterized in that: The ratio of the number of live bacteria of Saccharomyces cerevisiae numbered CICC 30241 and Bacillus subtilis numbered CICC 22062 in the fermentation bacteria is 0.2-0.5:

1.

9. The preparation method according to claim 3, characterized in that: The fermentation temperature is 30-40°C and the fermentation time is 70-120 hours.

10. The preparation method according to claim 3, characterized in that: The mass of ethanol added is 0.25-0.45 times the mass of water added in step I.

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