Determination equipment and determination method for pathogenicity of diaphorina citri entomogenous fungi
By using water storage tray, ventilation holes and mesh plates in the fungus pathogenicity determination equipment of citrus psyllia, the problems of weak Jiulixiang and difficulty in removing lice are solved, and the accuracy of the experiment and operation convenience are improved .
Patent Information
- Application Number
- CN202411388565.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-08
- Publication Date
- 2025-01-03
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the prior art, Jiulixiang is prone to weakening in the equipment for determining the pathogenicity of fungi of citrus psyllids, affecting the palatability of citrus psyllids, causing citrus psyllids to die early due to lack of nutrition, and the test cup design is difficult to survive effectively. and remove lice, affecting the accuracy of experimental results.
A device including a measuring cup, water storage tray, water absorption cotton, ventilation holes and mesh plates is designed to keep the culture medium moist by supporting legs and water storage tray, and the ventilation holes increase air circulation. The mesh plate is designed to facilitate the removal of lice and Prevent flight out, limit rod and snap ring structure stabilize and isolate lice range.
It improves the survival rate and experimental accuracy of Jiulixiang, ensures the normal death and removal of citrus psyllids, avoids the problems of lice flying out and equipment dumping, and improves the convenience of experimental operations.
Smart Images
Figure CN119242429A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of measuring equipment, in particular to a measuring equipment and a measuring method for the pathogenicity of citrus psyllid entomogenous fungi. Background Art
[0002] Citrus psyllids harm the tender branches and leaves of navel oranges. Adults often feed on tender shoots and leaf backs, while nymphs gather on tender shoots and leaves to suck. The damaged tender shoots and buds wither and shrink, and new leaves become twisted and deformed. Nymphs often excrete white flocculent secretions that cover the areas where the insects move. Psyllids can also induce sooty disease, leading to weak tree vigor and reduced yield and fruit quality. Psyllids are also important vectors of Huanglongbing, which is extremely harmful to citrus production.
[0003] In the prior art, a Chinese patent document with publication number CN112430639A proposes a method for determining the pathogenicity of citrus psyllid entomogenous fungi, which solves the problem of raising citrus psyllids in insect cages and then inoculating entomogenous fungi in Murraya aromatica potted plants. Because the volume of the insect cage is too large and the citrus psyllids are too small, it is not easy to observe and count the deaths of citrus psyllids. However, in actual application, the leaves of Murraya aromatica are relatively delicate and easy to rot, and excessive humidity can easily cause diseases in Murraya aromatica. Therefore, the above patent proposes that maintaining the relative humidity in the measuring cup to reach more than 95% will be difficult to ensure the normal survival of Murraya aromatica, and the cultivation of Murraya aromatica requires appropriate It needs suitable humidity and nutrients, and also likes to grow in a relatively ventilated environment. Therefore, it is not easy for the sweet olive in the test cup to survive normally, which leads to the weakening of the sweet olive, affects the palatability of the citrus psyllid, and causes the citrus psyllid to die prematurely due to lack of nutrition, affecting the accuracy of the experimental results. The citrus psyllid that died during the experiment needs to be taken out in time for measurement, but the citrus psyllid that is still alive in the test cup may fly out at this time, affecting the staff to pick up the dead citrus psyllid. Therefore, we disclose a measurement device and method for the pathogenicity of citrus psyllid fungi to meet the needs of the normal survival of the sweet olive in the test cup. Summary of the invention
[0004] 1. Technical issues to be resolved
[0005] In view of the shortcomings of the prior art, the present invention provides a device and method for determining the pathogenicity of citrus psyllid entomogenous fungi, which has the advantages of ensuring the normal survival of the sweet olive in the measuring cup and improving the accuracy of the experiment, and solves a series of problems such as the weakening of the sweet olive in the measuring cup, affecting the palatability of the citrus psyllid, and causing the citrus psyllid to die prematurely due to lack of nutrition.
[0006] (II) Technical solution
[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a device for measuring the pathogenicity of citrus psyllid entomogenous fungi, comprising a measuring cup, a mesh cover is movably sleeved on the top of the measuring cup, a plurality of supporting legs are fixedly connected to the top of the measuring cup, a water storage tray is placed at the lower end of the supporting legs, a water absorbent cotton is fixedly connected to the bottom end of the measuring cup, ventilation holes are provided on both sides of the middle of the measuring cup, a first mesh plate is rotatably connected to the upper end of the ventilation hole, a first baffle plate and a second baffle plate are fixedly connected to the upper and lower ends of the ventilation hole, respectively, the first baffle plate and the second baffle plate are both fitted with the first mesh plate, a culture matrix is placed at the bottom end of the measuring cup, a light-colored absorbent paper is laid on the upper end of the culture matrix, and the first mesh plate is located at the upper end of the light-colored absorbent paper; a plurality of air holes are provided at the bottom end of the measuring cup, the height of the water storage tray is higher than the height of the supporting legs, the side of the water absorbent cotton is U-shaped, and the two ends below the water absorbent cotton are both located in the water storage tray.
[0008] Preferably, two Osmanthus fragrans are planted in the culture matrix, the upper ends of the Osmanthus fragrans pass through the light-colored absorbent paper to the upper end of the measuring cup, the Osmanthus fragrans are located in the measuring cup, and the Osmanthus fragrans are both located on the longitudinal center axis of the measuring cup.
[0009] Preferably, the measuring cup is rotatably connected to a second mesh plate on both sides, one end of the second mesh plate is fixedly connected to a rotating shaft, one end of the rotating shaft passes through the outside of the measuring cup and is fixedly connected to a connecting plate, one end of the connecting plate is fixedly connected to a spring, one end of the spring is fixedly connected to a handle, one end of the handle is fixedly connected to a limiting rod, both ends of one side of the measuring cup are provided with moving grooves, and both upper and lower ends of the moving groove are provided with limiting grooves, the limiting rod passes through the spring and the connecting plate and then movably engages with the limiting grooves, and the two second mesh plates are respectively located on both sides of the two Osmanthus fragrans.
[0010] Preferably, the limiting groove is connected with the movable groove, the front of the movable groove is an arc with the rotating shaft as the center, the side of the movable groove is T-shaped, the limiting rod is T-shaped, the limiting rod is adapted to the movable groove, the limiting rod can be moved from the inside into the movable groove, and the limiting rod is located in the middle of the handle.
[0011] Preferably, a fourth baffle is fixedly connected to both ends of the measuring cup, a third baffle is fixedly connected to the middle of the measuring cup, the third baffle is located between the two fourth baffles, the two Murraya odoratas are respectively located at both ends of the third baffle, a clamping ring is placed at the upper end between the third baffle and the fourth baffle, and the clamping ring is adapted to the Murraya odorata.
[0012] Preferably, both sides of the third baffle and the fourth baffle are in contact with the top of the second mesh plate, the third baffle and the fourth baffle are both arc-shaped plates, and the lower end of the third baffle is fixedly connected to a support rod, which passes through the culture matrix and the light-colored absorbent paper and is fixedly connected to the measuring cup.
[0013] Preferably, the clamping ring comprises two semicircular rings, and the two semicircular rings are rotatably connected.
[0014] Preferably, one of the semicircular rings is rotatably connected to a connecting rod, and the other semicircular ring is fixedly connected to a limiting block, the connecting rod is an L-shaped rod, the limiting block is movably engaged with the connecting rod, and the lower end of the clamping ring is arc-shaped and fits with the top surface of the fourth baffle.
[0015] Preferably, the second mesh plate is in contact with the inner wall of the measuring cup, the leaves of the Murraya osmanthus are located between the second mesh plate and the mesh cover, and the lower end of the first mesh plate is triangular and does not fit the bottom end of the ventilation hole.
[0016] A method for determining the pathogenicity of citrus psyllid entomogenous fungi, specifically applied to a device for determining the pathogenicity of citrus psyllid entomogenous fungi as described above, comprises the following steps:
[0017] S1. Place two Osmanthus fragrans between the fourth baffle plate and the third baffle plate at the bottom of the measuring cup, then fill the bottom of the measuring cup with an appropriate amount of culture matrix, then lay a layer of light-colored absorbent paper on the upper end of the culture matrix, pull the handle to drive the limit rod from the limit groove to the movable groove, then rotate 90 degrees, so that the second mesh plate is driven by the connecting plate and the rotating shaft to rotate to a horizontal position and fit with the inner wall of the measuring cup, so that the leaves of Osmanthus fragrans are located between the second mesh plate and the mesh cover, and then place the citrus psyllid on the upper end of the second mesh plate, and then cover the mesh cover to limit the citrus psyllid in the measuring cup;
[0018] S2, when the citrus psyllid dies, it will fall to the upper end of the second screen plate, and at this time, the relative handle is operated to drive the second screen plate to rotate 90 degrees, so that the citrus psyllid slides down the second screen plate to the upper end of the light-colored absorbent paper under the influence of its own gravity, and then the second screen plate is rotated to the original position;
[0019] S3. Rotate the corresponding first screen plate to open the measuring cup, and pick up the citrus psyllids that fall from the upper end of the light-colored absorbent paper.
[0020] (III) Beneficial effects
[0021] Compared with the prior art, the present invention provides a device and method for determining the pathogenicity of citrus psyllid entomogenous fungi, which has the following beneficial effects:
[0022] 1. The measuring device and method for the pathogenicity of citrus psyllid insect-borne fungi, wherein the measuring cup is located in a water storage tray through supporting legs, so that the culture medium in the measuring cup absorbs water in the water storage tray through absorbent cotton, so that the culture medium remains moist and does not cause the root of Murraya odorifera to rot, and the staff can add nutrients to the water storage tray to promote the normal survival of Murraya odorifera, and ventilation holes are opened on both sides of the middle of the measuring cup, and the upper end of the ventilation hole is rotatably connected to a first mesh plate, so that the middle of the measuring cup can realize airflow through the first mesh plate, so that the air in the inner cavity of the measuring cup can circulate, thereby increasing the survival rate of Murraya odorifera, and after the citrus psyllid insect dies, it is necessary to take out the nutrient solution. When taking out, the first mesh plate can be opened and the citrus psyllid can be taken out directly, avoiding the problem of difficulty in taking out due to the small size of the measuring cup and the deep depth of the measuring cup. The staff takes out the citrus psyllid, which is convenient for operation. After the first mesh plate is closed and naturally droops, it fits with the first baffle plate and the second baffle plate to prevent the citrus psyllid from flying out from the gap between the first mesh plate and the measuring cup. Light-colored absorbent paper is laid on the upper end of the culture matrix, and the first mesh plate is located on the upper end of the light-colored absorbent paper, which is convenient for finding and taking out the citrus psyllid. A number of air holes are opened at the bottom of the measuring cup to make the culture matrix in the inner cavity of the measuring cup more breathable, which is conducive to the survival of the sweet olive.
[0023] 2. The device and method for determining the pathogenicity of the fungus borne by citrus psyllids include: when the handle is moved, the limit rod is first pulled to move from the limit groove to the movable groove; then, when the handle is rotated, the handle drives the connecting plate to move through the limit rod, so that the rotating shaft and the second mesh plate rotate, so that the moving position of the limit rod is limited by the movable groove, thereby limiting the moving position of the connecting plate, ensuring the stability of the rotating shaft during rotation, and making the position of the second mesh plate relatively stable before and after rotation, not easy to shake, and convenient for use; the two second mesh plates are respectively located on both sides of the two osmanthus trees, so that the upper and lower ends of the second mesh plates are separated, limiting the activity range of the citrus psyllids, so that when the staff takes the dead citrus psyllids, the surviving citrus psyllids will not fly out, and it is convenient for the staff to pick up the dead citrus psyllids.
[0024] 3. The device and method for determining the pathogenicity of the fungus of citrus psyllids. After the Murraya odorata passes between the third baffle and the fourth baffle, it can be placed at the bottom of the measuring cup, so that the clamp ring can fill the gap between the third baffle, the fourth baffle and Murraya odorata, so as to prevent the citrus psyllids from flying from between the third baffle and the fourth baffle to the lower end of the second mesh plate. After the dead citrus psyllids fall to the fourth baffle or the upper end of the third baffle, they can slide to the upper end of the second mesh plate. The clamp ring includes two semicircular rings, and the two semicircular rings Rotating connection, one semicircular ring is rotatably connected with a connecting rod, and the other semicircular ring is fixedly connected with a limiting block. The connecting rod is an L-shaped rod, and the limiting block is movably clamped with the connecting rod. The lower end of the clamp is arc-shaped and fits with the top surface of the fourth baffle, so that the clamp is easy to fit with the outer wall of the sweet olive, which is convenient to operate, and the clamp and the sweet olive fit better, preventing citrus psyllids from flying to the lower end of the second mesh plate through the gap between the clamp and the sweet olive, so that the second mesh plate can better separate the inner cavity space of the measuring cup. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;
[0026] Figure 2 It is a partially cutaway three-dimensional structural schematic diagram of the water storage tray of the present invention;
[0027] Figure 3 It is a schematic diagram of a partially cutaway three-dimensional structure of a measuring cup of the present invention;
[0028] Figure 4 It is a schematic diagram of the three-dimensional structure of the connection between the third baffle plate, the fourth baffle plate and the clamping ring of the present invention;
[0029] Figure 5 For the present invention Figure 4 A is an enlarged schematic diagram of the three-dimensional structure;
[0030] Figure 6 It is a schematic diagram of the three-dimensional structure of the connection of the first mesh plate, the first baffle plate and the second baffle plate of the present invention;
[0031] Figure 7 It is a schematic diagram of the three-dimensional structure of the connection between the connecting rod and the limiting block of the present invention;
[0032] Figure 8 It is a schematic diagram of a partially cutaway three-dimensional structure of the clamping ring of the present invention.
[0033] In the figure: 1. measuring cup; 2. mesh cover; 3. supporting legs; 4. water storage tray; 5. air holes; 6. absorbent cotton; 7. first mesh plate; 8. first baffle plate; 9. second baffle plate; 10. ventilation holes; 11. culture matrix; 12. light-colored absorbent paper; 13. sweet olive; 14. second mesh plate; 15. rotating shaft; 16. connecting plate; 17. spring; 18. handle; 19. limit rod; 20. limit slot; 21. moving slot; 22. third baffle plate; 23. fourth baffle plate; 24. retaining ring; 25. connecting rod; 26. limit block. DETAILED DESCRIPTION
[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0035] As introduced in the background technology, in order to solve the deficiencies existing in the prior art, the present application proposes a device and method for determining the pathogenicity of citrus psyllid entomogenous fungi.
[0036] In a typical implementation of the present application, Figure 1-Figure 8As shown, a device for determining the pathogenicity of citrus psyllid entomogenous fungi comprises a measuring cup 1, a mesh cover 2 is movably sleeved on the top of the measuring cup 1, a plurality of supporting legs 3 are fixedly connected to the top of the measuring cup 1, a water storage tray 4 is placed at the lower end of the supporting legs 3, a water-absorbing cotton 6 is fixedly connected to the bottom end of the measuring cup 1, ventilation holes 10 are opened on both sides of the middle of the measuring cup 1, a first mesh plate 7 is rotatably connected to the upper end of the ventilation hole 10, a first baffle plate 8 and a second baffle plate 9 are fixedly connected to the upper and lower ends of the ventilation hole 10, respectively, the first baffle plate 8 and the second baffle plate 9 are both fitted with the first mesh plate 7, a culture matrix 11 is placed at the bottom end of the measuring cup 1, a light-colored absorbent paper 12 is laid on the upper end of the culture matrix 11, and the first mesh plate 7 is located at the upper end of the light-colored absorbent paper 12;A plurality of air holes 5 are provided at the bottom of the measuring cup 1, the height of the water storage tray 4 is higher than the height of the supporting legs 3, the side of the absorbent cotton 6 is U-shaped, and both ends of the absorbent cotton 6 are located in the water storage tray 4, two Murraya odorata 13 are planted in the culture matrix 11, and the upper end of Murraya odorata 13 passes through the light-colored absorbent paper 12 to the upper end of the measuring cup 1, and Murraya odorata 13 is located in the measuring cup 1, and Murraya odorata 13 is located on the longitudinal center axis of the measuring cup 1, and the lower end of the first mesh plate 7 is triangular and does not fit with the bottom end of the ventilation hole 10. When in use, a mesh cover 2 is movably sleeved through the top of the measuring cup 1, so that the citrus psyllids in the measuring cup 1 will not fly out, and a plurality of branches are fixedly connected through the top of the measuring cup 1. The support legs 3 are provided with a water storage tray 4 at the lower end of the support legs 3, and the bottom end of the measuring cup 1 is fixedly connected with a water-absorbing cotton 6, and a culture matrix 11 is placed at the bottom end of the measuring cup 1. The side of the water-absorbing cotton 6 is U-shaped, and the two ends below the water-absorbing cotton 6 are both located in the water storage tray 4, so that the measuring cup 1 is located in the water storage tray 4 through the support legs 3, so that the culture matrix 11 in the measuring cup 1 absorbs water in the water storage tray 4 through the water-absorbing cotton 6, so that the culture matrix 11 remains moist and does not cause the root of the sweet olive 13 to rot, and the staff can add nutrients to the water storage tray 4 to promote the normal survival of the sweet olive 13, and ventilation holes 10 are opened on both sides of the middle of the measuring cup 1, and the upper end of the ventilation hole 10 is rotatably connected with a first The mesh plate 7 allows air to pass through the middle of the measuring cup 1 through the first mesh plate 7, so that the air in the inner cavity of the measuring cup 1 can circulate, increasing the survival rate of the Murraya osmanthus 13, and when the citrus psyllids need to be taken out after they die, the first mesh plate 7 can be opened and taken out directly, avoiding the problem of difficulty in taking out the citrus psyllids due to the deep depth of the measuring cup 1 and the small size of the citrus psyllids. The staff can take out the citrus psyllids, which is convenient for operation. The upper and lower ends of the ventilation hole 10 are respectively fixedly connected with the first baffle plate 8 and the second baffle plate 9, and the first baffle plate 8 and the second baffle plate 9 are both fitted with the first mesh plate 7, so that when the first mesh plate 7 is closed and naturally droops, it fits with the first baffle plate 8 and the second baffle plate 9 to prevent the citrus psyllids from The gap between the first mesh plate 7 and the measuring cup 1 flies out, and a light-colored absorbent paper 12 is laid on the upper end of the culture matrix 11. The first mesh plate 7 is located at the upper end of the light-colored absorbent paper 12, which is convenient for finding and removing citrus psyllids. A plurality of air holes 5 are opened at the bottom of the measuring cup 1, so that the culture matrix 11 in the inner cavity of the measuring cup 1 has better air permeability, which is conducive to the survival of the sweet olive 13. The height of the water storage tray 4 is higher than the height of the supporting legs 3, so that the water storage tray 4 can limit the position of the measuring cup 1 and reduce the occurrence of the tipping of the measuring cup 1. The lower end of the first mesh plate 7 is triangular and does not fit the bottom of the ventilation hole 10, so that the first mesh plate 7 is easy for the staff to move and use. ;
[0037] As a preferred implementation in this embodiment, refer to the attached Figure 4 and Figure 5, the measuring cup 1 is rotatably connected with a second mesh plate 14 on both sides, one end of the second mesh plate 14 is fixedly connected with a rotating shaft 15, one end of the rotating shaft 15 penetrates to the outside of the measuring cup 1 and is fixedly connected with a connecting plate 16, one end of the connecting plate 16 is fixedly connected with a spring 17, one end of the spring 17 is fixedly connected with a handle 18, one end of the handle 18 is fixedly connected with a limiting rod 19, both ends of one side of the measuring cup 1 are provided with a moving groove 21, and both upper and lower ends of the moving groove 21 are provided with a limiting groove 20, the limiting rod 19 passes through the spring 17 and the connecting plate 16 and then is movably engaged with the limiting groove 20, and the two The second mesh plates 14 are respectively located on both sides of the two Osmanthus fragrans 13, the limiting groove 20 is connected with the moving groove 21, the front of the moving groove 21 is an arc with the rotating shaft 15 as the center, the side of the moving groove 21 is T-shaped, the limiting rod 19 is T-shaped, the limiting rod 19 is adapted to the moving groove 21, the limiting rod 19 can be moved from the inside to the moving groove 21, the limiting rod 19 is located in the middle of the handle 18, the second mesh plate 14 is fitted with the inner wall of the measuring cup 1, the leaves of the Osmanthus fragrans 13 are located between the second mesh plate 14 and the mesh cover 2, and the second mesh plate 14 is rotatably connected to both sides of the measuring cup 1, and the second mesh plate 14 is One end of the plate 14 is fixedly connected to a rotating shaft 15, one end of the rotating shaft 15 passes through the outside of the measuring cup 1 and is fixedly connected to a connecting plate 16, one end of the connecting plate 16 is fixedly connected to a spring 17, one end of the spring 17 is fixedly connected to a handle 18, one end of the handle 18 is fixedly connected to a limiting rod 19, both ends of one side of the measuring cup 1 are provided with a moving groove 21, and both upper and lower ends of the moving groove 21 are provided with a limiting groove 20, the limiting rod 19 passes through the spring 17 and the connecting plate 16 and then is movably connected with the limiting groove 20, so that when the handle 18 moves, the limiting rod 19 is first pulled to move from the limiting groove 20 To the movable groove 21, and then when the handle 18 is rotated, it drives the connecting plate 16 to move through the limit rod 19, so that the rotating shaft 15 and the second mesh plate 14 are rotated, so that the moving position of the limit rod 19 is limited by the movable groove 21, thereby limiting the moving position of the connecting plate 16, ensuring the stability of the rotating shaft 15 during rotation, and through the two second mesh plates 14 are respectively located on both sides of the two sweet olives 13, so that the upper and lower ends of the second mesh plates 14 are separated, limiting the activity range of the citrus psyllids, so that when the staff takes the dead citrus psyllids, the surviving citrus psyllids will not fly out.
[0038] As a preferred implementation in this embodiment, refer to the attached Figure 3 , Figure 4 , Figure 6-Figure 8, both ends of the measuring cup 1 are fixedly connected with a fourth baffle 23, the middle part of the measuring cup 1 is fixedly connected with a third baffle 22, the third baffle 22 is located between the two fourth baffles 23, the two Murraya 13 are respectively located at both ends of the third baffle 22, a clamping ring 24 is placed at the upper end between the third baffle 22 and the fourth baffle 23, the clamping ring 24 is adapted to the Murraya 13, both sides of the third baffle 22 and the fourth baffle 23 are fitted with the top of the second mesh plate 14, the fourth baffle 23 and the fourth baffle 23 are both arc-shaped plates, the lower end of the third baffle 22 is fixedly connected with a support rod, the support rod passes through the culture matrix 11 and the light-colored absorbent paper 12 and is fixedly connected to the measuring cup 1, the clamping ring 24 includes two semicircular rings, the two semicircular rings are rotatably connected, and one semicircular ring is rotatably connected A connecting rod 25 is connected, and another semicircular ring is fixedly connected to a limited block 26. The connecting rod 25 is an L-shaped rod. The limited block 26 is movably connected to the connecting rod 25. The lower end of the clamping ring 24 is arc-shaped and fits with the top surface of the fourth baffle 23. The fourth baffle 23 is fixedly connected to both ends of the measuring cup 1. The middle part of the measuring cup 1 is fixedly connected to the third baffle 22. The third baffle 22 is located between the two fourth baffles 23. The two osmanthus fragrans 13 are respectively located at the two ends of the third baffle 22. A clamping ring 24 is placed on the upper end between the third baffle 22 and the fourth baffle 23. The clamping ring 24 is adapted to the osmanthus fragrans 13, so that the osmanthus fragrans 13 can be placed at the bottom of the measuring cup 1 after passing between the third baffle 22 and the fourth baffle 23, so that the clamping ring 24 can make up for the third baffle 2 2. The gap between the fourth baffle plate 23 and the osmanthus fragrans 13 is formed by both sides of the third baffle plate 22 and the fourth baffle plate 23 being fitted with the top of the second mesh plate 14 to prevent the citrus psyllids from flying from between the third baffle plate 22 and the fourth baffle plate 23 to the lower end of the second mesh plate 14. The fourth baffle plate 23 and the fourth baffle plate 23 are both arc-shaped plates, so that the dead citrus psyllids fall to the fourth baffle plate 23 or the upper end of the third baffle plate 22 and can slide to the upper end of the second mesh plate 14. The clamping ring 24 includes two semicircular rings, which are rotatably connected. One semicircular ring is rotatably connected to a connecting rod 25, and the other semicircular ring is fixedly connected to a limiting block 26. The connecting rod 25 is an L-shaped rod. The limiting block 26 is movably connected to the connecting rod 25. The lower end of the clamping ring 24 is arc-shaped. And it fits with the top surface of the fourth baffle plate 23, so that the clamping ring 24 is easy to fit with the outer wall of the Murraya 13, which is convenient to operate, so that the clamping ring 24 and the Murraya 13 fit better, and prevent the citrus psyllids from flying to the lower end of the second mesh plate 14 through the gap between the clamping ring 24 and the Murraya 13, so that the second mesh plate 14 can better separate the inner cavity space of the measuring cup 1, so that when the citrus psyllids fall to the top of the second mesh plate 14 after death, the citrus psyllids are moved to the lower end of the second mesh plate 14 through the rotation of the second mesh plate 14, and then the second mesh plate 14 is restored to its original position. When picking up the citrus psyllids at the lower end of the second mesh plate 14, the surviving citrus psyllids at the upper end will not fly out, which is conducive to picking up the dead citrus psyllids and convenient for the staff to operate.
[0039] A method for determining the pathogenicity of citrus psyllid entomogenous fungi, specifically applied to a device for determining the pathogenicity of citrus psyllid entomogenous fungi as described above, comprises the following steps:
[0040] S1, place two Osmanthus fragrans 13 between the fourth baffle plate 23 and the third baffle plate 22 at the bottom of the measuring cup 1, then fill the bottom of the measuring cup 1 with an appropriate amount of culture matrix 11, then lay a layer of light-colored absorbent paper 12 on the upper end of the culture matrix 11, pull the handle 18 to drive the limit rod 19 from the limit groove 20 to the movable groove 21, then rotate ninety degrees, so that the second mesh plate 14 is driven by the connecting plate 16 and the rotating shaft 15 to rotate to a horizontal position and fit with the inner wall of the measuring cup 1, so that the leaves of Osmanthus fragrans 13 are located between the second mesh plate 14 and the mesh cover 2, and then place the citrus psyllid on the upper end of the second mesh plate 14, then cover the mesh cover 2, and limit the citrus psyllid to be located in the measuring cup 1;
[0041] S2, when the citrus psyllid dies, it will fall to the upper end of the second mesh plate 14, and at this time, the handle 18 relative to the citrus psyllid is operated to drive the second mesh plate 14 to rotate 90 degrees, so that the citrus psyllid slides down the second mesh plate 14 to the upper end of the light-colored absorbent paper 12 under the influence of its own gravity, and then the second mesh plate 14 is rotated to the original position;
[0042] S3. Rotate the corresponding first screen plate 7 to open the measuring cup 1, pick up the citrus psyllids dropped from the upper end of the light-colored absorbent paper 12, so that the measuring cup 1 is more ventilated, and can provide suitable humidity and nutrients for the Murraya osmanthus 13, which is beneficial to the normal survival of the Murraya osmanthus 13 and is not easy to weaken. It is convenient to pick up the citrus psyllids after they die, and the living citrus psyllids will not fly out when picking them up, which is beneficial to the operation of the staff.
[0043] The working principle of the present invention is as follows: when in use, the measuring cup 1 is located in the water storage tray 4 through the supporting legs 3, so that the culture matrix 11 in the measuring cup 1 absorbs the water in the water storage tray 4 through the absorbent cotton 6, so that the culture matrix 11 remains moist and does not cause the root of the Murraya 13 to rot, and the staff can add nutrients to the water storage tray 4 to promote the normal survival of the Murraya 13. Ventilation holes 10 are provided on both sides of the middle of the measuring cup 1, and the upper end of the ventilation hole 10 is rotatably connected to the first mesh plate 7, so that the middle of the measuring cup 1 can realize airflow through the first mesh plate 7, so that the air in the inner cavity of the measuring cup 1 can circulate, thereby increasing the survival rate of the Murraya 13, and when the citrus psyllids die and need to be taken out, they can be The first mesh plate 7 can be opened and taken out directly to avoid the problem of difficulty in taking out the citrus psyllid due to the deep depth of the measuring cup 1 and the small size of the citrus psyllid. The staff can take out the citrus psyllid, which is convenient for operation. After the first mesh plate 7 is closed and naturally droops, it fits with the first baffle plate 8 and the second baffle plate 9 to prevent the citrus psyllid from flying out of the gap between the first mesh plate 7 and the measuring cup 1. A light-colored absorbent paper 12 is laid on the upper end of the culture matrix 11, and the first mesh plate 7 is located on the upper end of the light-colored absorbent paper 12, which is convenient for finding and taking out the citrus psyllid. A plurality of air holes 5 are opened at the bottom end of the measuring cup 1, so that the culture matrix 11 in the inner cavity of the measuring cup 1 has better air permeability, which is conducive to the survival of the sweet olive 13.
[0044] When the handle 18 moves, the limit rod 19 is first pulled to move from the limit groove 20 to the moving groove 21. Then, when the handle 18 is rotated, the connecting plate 16 is driven to move through the limit rod 19, so that the rotating shaft 15 and the second mesh plate 14 are rotated, so that the moving position of the limit rod 19 is limited by the moving groove 21, thereby limiting the moving position of the connecting plate 16, ensuring the stability of the rotating shaft 15 during rotation, so that the position of the second mesh plate 14 before and after rotation is relatively stable and not easy to shake, which is convenient for use. The two second mesh plates 14 are respectively located on both sides of the two osmanthus trees 13, so that the upper and lower ends of the second mesh plates 14 are separated, limiting the activity range of the citrus psyllids, so that when the staff takes the dead citrus psyllids, the surviving citrus psyllids will not fly out, which is convenient for the staff to pick up the dead citrus psyllids.
[0045] After the Osmanthus fragrans 13 passes through the third baffle plate 22 and the fourth baffle plate 23, it can be placed at the bottom of the measuring cup 1, so that the clamp ring 24 can fill the gap between the third baffle plate 22, the fourth baffle plate 23 and the Osmanthus fragrans 13, so as to prevent the citrus psyllids from flying from between the third baffle plate 22 and the fourth baffle plate 23 to the lower end of the second mesh plate 14. After the dead citrus psyllids fall to the fourth baffle plate 23 or the upper end of the third baffle plate 22, they can slide to the upper end of the second mesh plate 14. The clamp ring 24 includes two semicircular rings, the two semicircular rings are rotatably connected, and one semicircular ring is rotatably connected. It is connected to a connecting rod 25, and another semicircular ring is fixedly connected to a limiting block 26. The connecting rod 25 is an L-shaped rod, and the limiting block 26 is movably connected to the connecting rod 25. The lower end of the clamping ring 24 is arc-shaped and fits with the top surface of the fourth baffle 23, so that the clamping ring 24 is easy to fit with the outer wall of the Murraya osmanthus 13, which is convenient to operate, so that the clamping ring 24 and the Murraya osmanthus 13 fit better, preventing citrus psyllids from flying to the lower end of the second mesh plate 14 through the gap between the clamping ring 24 and the Murraya osmanthus 13, so that the second mesh plate 14 can better separate the inner cavity space of the measuring cup 1.
[0046] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A device for measuring the pathogenicity of citrus psyllid entomogenous fungi, characterized in that: The measuring cup (1) comprises a measuring cup (1), the top of which is movably sleeved with a mesh cover (2), the top of which is fixedly connected with a plurality of supporting legs (3), the lower end of which is provided with a water storage tray (4), the bottom of which is fixedly connected with a water absorbent cotton (6), ventilation holes (10) are provided on both sides of the middle of the measuring cup (1), the upper end of which is rotatably connected with a first mesh plate (7), the upper and lower ends of which are respectively fixedly connected with a first baffle plate (8) and a second baffle plate (9), the first baffle plate (8) and the second baffle plate (9) are both fitted with the first mesh plate (7), a culture matrix (11) is placed at the bottom of the measuring cup (1), the upper end of which is provided with a light-colored absorbent paper (12), and the first mesh plate (7) is located at the upper end of the light-colored absorbent paper (12); A plurality of air holes (5) are provided at the bottom of the measuring cup (1); the height of the water storage tray (4) is higher than the height of the supporting legs (3); the side surface of the absorbent cotton (6) is U-shaped; both ends of the lower side of the absorbent cotton (6) are located inside the water storage tray (4).
2. The device for measuring the pathogenicity of citrus psyllid entomogenous fungi according to claim 1, characterized in that: Two Osmanthus fragrans (13) are planted in the culture matrix (11), and the upper ends of the Osmanthus fragrans (13) pass through the light-colored absorbent paper (12) to the upper end of the measuring cup (1). The Osmanthus fragrans (13) are located in the measuring cup (1), and the Osmanthus fragrans (13) are located on the longitudinal center axis of the measuring cup (1).
3. The device for measuring the pathogenicity of citrus psyllid entomogenous fungi according to claim 2, characterized in that: The measuring cup (1) is rotatably connected to a second mesh plate (14) on both sides thereof, one end of the second mesh plate (14) is fixedly connected to a rotating shaft (15), one end of the rotating shaft (15) passes through the outside of the measuring cup (1) and is fixedly connected to a connecting plate (16), one end of the connecting plate (16) is fixedly connected to a spring (17), one end of the spring (17) is fixedly connected to a handle (18), one end of the handle (18) is fixedly connected to a limiting rod (19), both ends of one side of the measuring cup (1) are provided with a moving groove (21), both upper and lower ends of the moving groove (21) are provided with a limiting groove (20), the limiting rod (19) passes through the spring (17) and the connecting plate (16) and then is movably engaged with the limiting groove (20), and the two second mesh plates (14) are respectively located on both sides of the two Osmanthus fragrans (13).
4. The device for measuring the pathogenicity of citrus psyllid entomogenous fungi according to claim 3, characterized in that: The limiting groove (20) is connected to the movable groove (21); the front of the movable groove (21) is an arc with the rotating shaft (15) as the center; the side of the movable groove (21) is T-shaped; the limiting rod (19) is T-shaped; the limiting rod (19) is adapted to the movable groove (21); the limiting rod (19) can be moved from the inside into the movable groove (21); and the limiting rod (19) is located in the middle of the handle (18).
5. The device for measuring the pathogenicity of citrus psyllid entomogenous fungi according to claim 4, characterized in that: The measuring cup (1) is fixedly connected to both ends with a fourth baffle (23), the middle of the measuring cup (1) is fixedly connected with a third baffle (22), the third baffle (22) is located between the two fourth baffles (23), the two Osmanthus fragrans (13) are respectively located at the two ends of the third baffle (22), a snap ring (24) is placed at the upper end between the third baffle (22) and the fourth baffle (23), and the snap ring (24) is adapted to the Osmanthus fragrans (13).
6. The device for measuring the pathogenicity of citrus psyllid entomogenous fungi according to claim 5, characterized in that: Both sides of the third baffle plate (22) and the fourth baffle plate (23) are in contact with the top of the second mesh plate (14); the fourth baffle plate (23) and the fourth baffle plate (23) are both arc-shaped plates; the lower end of the third baffle plate (22) is fixedly connected to a support rod; the support rod penetrates the culture matrix (11) and the light-colored absorbent paper (12) and is fixedly connected to the measuring cup (1).
7. The device for measuring the pathogenicity of citrus psyllid entomogenous fungi according to claim 6, characterized in that: The clamping ring (24) comprises two semicircular rings, and the two semicircular rings are rotatably connected.
8. The device for measuring the pathogenicity of citrus psyllid entomogenous fungi according to claim 7, characterized in that: One of the semicircular rings is rotatably connected to a connecting rod (25), and the other semicircular ring is fixedly connected to a limiting block (26); the connecting rod (25) is an L-shaped rod; the limiting block (26) is movably engaged with the connecting rod (25); and the lower end of the clamping ring (24) is arc-shaped and fits with the top surface of the fourth baffle (23).
9. The device for measuring the pathogenicity of citrus psyllid entomogenous fungi according to claim 3, characterized in that: The second mesh plate (14) is in contact with the inner wall of the measuring cup (1), the leaves of the Murraya osmanthus (13) are located between the second mesh plate (14) and the mesh cover (2), and the lower end of the first mesh plate (7) is triangular and does not fit the bottom end of the ventilation hole (10).
10. A method for determining the pathogenicity of citrus psyllid entomogenous fungi, specifically applied to the device for determining the pathogenicity of citrus psyllid entomogenous fungi according to any one of claims 1 to 9, characterized in that: The steps include: S1. Place two Osmanthus fragrans (13) between the fourth baffle plate (23) and the third baffle plate (22) at the bottom of the measuring cup (1), then fill the bottom of the measuring cup (1) with an appropriate amount of culture matrix (11), then lay a layer of light-colored absorbent paper (12) on the upper end of the culture matrix (11), pull the handle (18) to drive the limiting rod (19) from the limiting groove (20) to the movable groove (21), then rotate ninety degrees, so that the second mesh plate (14) is driven by the connecting plate (16) and the rotating shaft (15) to rotate to a horizontal position and fit with the inner wall of the measuring cup (1), so that the leaves of Osmanthus fragrans (13) are located between the second mesh plate (14) and the mesh cover (2), then place the citrus psyllid on the upper end of the second mesh plate (14), then cover the mesh cover (2) to limit the citrus psyllid in the measuring cup (1); S2. When the citrus psyllid dies, it will fall to the upper end of the second mesh plate (14). At this time, the handle (18) is operated to drive the second mesh plate (14) to rotate 90 degrees, so that the citrus psyllid slides down the second mesh plate (14) to the upper end of the light-colored absorbent paper (12) under the influence of its own gravity, and then the second mesh plate (14) is rotated to its original position; S3. Rotate the corresponding first screen plate (7) to open the inside of the measuring cup (1) and pick up the citrus psyllids that fall from the upper end of the light-colored absorbent paper (12).