Mycorrhizal seedling culture container convenient for experimental observation

Abstract

The invention discloses a mycorrhizal seedling culture container convenient for experimental observation. The nutrition cup is composed of a nutrition cup 1, a nutrition cup door 2, a nutrition cup door handle 3, water leakage holes 4, a black light-proof plastic box 5, a plastic plate 6 with holes, a plastic plate door 7 and a plastic plate door handle 8. The nutrition cups 1 are made of transparent plastic materials so that the growth condition of mycorrhiza can be observed at any time, water leakage holes 4 are formed in the cup bottom of each nutrition cup, an opening is formed in the cup wall, and a small door 2 capable of being opened and closed is arranged at the opening so that plant lateral roots can be conveniently taken for experimental observation. The nutrition cup 1 is placed through eight circular openings in the plastic plate 6 with holes, a rectangular opening in the middle of the plastic plate 6 is used for observing watering conditions, and a small door 7 capable of being opened and closed is arranged at the opening. The seedling culture container is placed in the black light-proof plastic box 5 in the mycorrhizal seedling culture process so as to simulate the dark environment in the soil. The novel mycorrhizal seedling culture container is suitable for observing the growth condition of mycorrhiza at any time in the mycorrhizal seedling culture process, sampling the root system of the mycorrhizal seedling at any time without damaging the whole seedling, and is beneficial to scientific research of the mycorrhizal seedling.

Description

Technical Field

[0001] The invention relates to a mycorrhizal seedling raising container which is convenient for test observation and is a novel seedling raising container belonging to the category of plant mycorrhizal seedling raising containers. Background Art

[0002] Mycorrhiza is a mutually beneficial association formed by mycorrhizal fungi and plant roots in the soil. It is a common phenomenon of fungi and plant symbiosis in nature. Mycorrhizal fungi can form symbiotic structures in the roots of host plants, thereby increasing the host plant's absorption and utilization of nutrients and water in the soil and carbohydrate metabolism, promoting plant growth and yield, improving the plant's tolerance to abiotic adversities such as high salt, drought, low temperature, and heavy metals, effectively enhancing the plant's resistance to a variety of diseases, and inducing plants to produce systemic resistance. In addition, mycorrhizal fungal infection can induce significant changes in plant roots, change the plant's root configuration, increase root branches, promote the formation of more lateral roots, increase total root length and root absorption area, thereby promoting the plant's absorption of water and nutrients and promoting plant growth. A large number of studies have shown that the use of excellent mycorrhizal fungi for inoculation and the cultivation of mycorrhizal seedlings can increase the plant's absorption of water and nutrients, improve plant stress resistance, and promote its growth and development. Because mycorrhizal seedlings have many excellent characteristics, people have been studying mycorrhizal fungi and mycorrhizal seedlings more and more in recent years. In the experiment, it is usually necessary to infect plants with pure culture agents of different strains and cultivate mycorrhizal seedlings to study the effects of different mycorrhizal fungi on different plants. During the cultivation of mycorrhizal seedlings, it is necessary to take root sections at different stages of mycorrhizal fungus inoculation to observe the mycorrhizal infection. However, the existing mycorrhizal seedling nursery containers are usually made of opaque plastic with small openings, and the depth of the container is not conducive to root extension. When using traditional nursery containers to cultivate mycorrhizal seedlings, the entire mycorrhizal seedling can only be taken out when observing the mycorrhizal growth and detecting the mycorrhizal infection rate, resulting in the loss of test samples. In order to save costs, the loss of test materials should be minimized during the test. If the mycorrhizal seedlings of rare seedlings are cultivated, the loss of seedlings should be minimized as much as possible. Therefore, it is very important to choose a mycorrhizal seedling nursery container that is convenient for experimental observation. Summary of the invention

[0003] In view of the above-mentioned shortcomings of the existing seedling containers, the purpose of the present invention is to provide a mycorrhizal seedling seedling container that is convenient for experimental observation. Cultivating mycorrhizal seedlings with the seedling container is beneficial to the growth of mycorrhizal seedlings and the extension of the root system, and is convenient for observing the mycorrhizal growth at any time during the growth of the mycorrhizal seedlings, and reduces the loss of seedlings during the experimental sampling process, which is conducive to the scientific research of mycorrhizal fungi and mycorrhizal seedlings.

[0004] The technical solution adopted by the present invention is: a novel mycorrhizal seedling nursery container, which is composed of a nutrient cup, a nutrient cup door, a nutrient cup door handle, a water leakage hole, a black opaque plastic box, a perforated plastic plate, a plastic plate door, and a plastic plate door handle. The nutrient cup is made of transparent plastic material so that the growth of mycorrhiza can be observed at any time. Each nutrient cup has a water leakage hole at the bottom, an opening on the cup wall, and a small door that can be opened and closed at the opening, which is convenient for taking the lateral roots of the plant for experimental observation. The nutrient cup is placed through 8 circular openings on the perforated plastic plate, and the rectangular opening in the middle of the plastic plate is used to observe the watering situation, and a small door that can be opened and closed is provided at the opening. The nursery container is placed in a black opaque plastic box during the cultivation of mycorrhizal seedlings to simulate the dark environment in the soil.

[0005] The above-mentioned nutrient cup is in the shape of a truncated cone with a larger top and a smaller bottom. The diameter of the cup mouth is 10 cm, and there is a cup edge with a width of 0.5 cm. The diameter of the cup bottom is 7 cm, and there is a circular water leakage hole with a diameter of 1 cm for easy ventilation and water permeability. The height is 20 cm, which is conducive to the extension of the root system of the mycorrhizal seedlings. A piece of permeable cloth can be padded on the inner side of the bottom of each nutrient cup to prevent the matrix in the nutrient cup from leaking out.

[0006] The above-mentioned nutrient cup is made of transparent plastic, which is convenient for observing the growth of mycorrhizal fungi. The cup wall has an opening, which is rectangular in shape and measures 5cm×6cm. There is a small door at the opening that can be opened and closed so that root segments can be taken from the opening for tests such as mycorrhizal infection rate determination.

[0007] The above-mentioned perforated plastic plate is made of black opaque plastic, with a size of 50cm×50cm, and has 8 circular openings with a diameter of 10cm and a rectangular opening with a size of 10cm×10cm. The plastic plate door is made of the same material as the perforated plastic plate.

[0008] The above-mentioned nutrient cup is placed through the 8 circular openings on the perforated plastic plate. The rectangular opening in the middle of the plastic plate is used to observe the watering situation. There is a small door with a size of 10cm×10cm that can be opened and closed at the opening, which is convenient for observing the watering situation at any time and preventing excessive watering.

[0009] During the cultivation of mycorrhizal seedlings, the above seedling container was placed in a black opaque plastic box to simulate the dark environment in the soil. The dimensions of the black opaque plastic box are: 46 cm long, 46 cm wide, and 23 cm high. If you need to observe the growth of mycorrhizae or sample the plant roots during the seedling cultivation process, you need to take the nutrient cup out of the plastic box. You can take out the nutrient cup alone or lift the perforated plastic plate and take out 8 nutrient cups at the same time, so that you can observe the growth of mycorrhizae at any time during the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 The figure is a three-dimensional schematic diagram of a mycorrhizal seedling raising container that is convenient for experimental observation.

[0011] Figure 2The following are the front view, side view and top view of a mycorrhizal seedling raising container which is convenient for experimental observation.

[0012] In the figure, 1-nutrition cup, 2-nutrition cup door, 3-nutrition cup door handle, 4-leakage hole, 5-black opaque plastic box, 6-plastic board with holes, 7-plastic board door, 8-plastic board door handle. DETAILED DESCRIPTION

[0013] The details and usage of the present invention are further explained below with reference to the accompanying drawings and specific implementation examples.

[0014] Implementation Case 1:

[0015] Adopt peat soil, perlite mixed matrix to be packed in each nutrient cup 1 of seedling raising container of the present invention, each nutrient cup is sown 3 healthy black pine seeds, watered after sowing, and plastic board door 7 can be opened to see watering situation.After black pine emerges, immediately inoculate the cultivated velvet sticky cap boletus liquid inoculum, inoculate 3 times in total, respectively after black pine emerges, 30d, 60d after emergence inoculation.After the last inoculation 10 days, nutrient cup 1 is taken out from black light-proof plastic box 5, observe mycorrhizal growth situation by the transparent cup wall of nutrient cup 1, and open the nutrient cup door 2 on nutrient cup, therefrom take out the lateral root of a section of black pine seedling, carry out the mensuration of mycorrhizal infection rate.Mycorrhizal growth situation can be observed at any time at each stage of black pine seedling growth.

[0016] Implementation Case 2:

[0017] The matrix prepared by mixing peat soil, perlite and vermiculite is loaded into each nutrient cup 1 of the seedling container of the present invention, and each nutrient cup is sown with a healthy walnut seed that has been germinated, and watered after sowing, and the plastic board door 7 can be opened to see the watering situation. After the walnut seedlings emerge, the two-color wax mushroom liquid inoculum that has been cultivated is inoculated immediately, and inoculated 3 times in total, respectively after the walnut seedlings emerge, 15d and 30d after the emergence. After the last inoculation for 10 days, the nutrient cup 1 is taken out from the black light-proof plastic box 5, and the mycorrhizal growth situation is observed by the transparent cup wall of the nutrient cup 1, and the nutrient cup door 2 on the nutrient cup is opened, and the lateral root of a section of walnut seedling is taken out therefrom, and the mycorrhizal infection rate is measured. The infection situation of mycorrhiza on the root system of the walnut seedling can be monitored in real time.

Claims

1. A mycorrhizal seedling container that is convenient for experimental observation, comprising a nutrient cup (1), a nutrient cup door (2), a nutrient cup door handle (3), a water leakage hole (4), a black light-proof plastic box (5), a perforated plastic plate (6), a plastic plate door (7), and a plastic plate door handle (8). The nutrient cup (1) is made of transparent plastic material so that the growth of mycorrhizae can be observed at any time. Each nutrient cup has a water leakage hole (4) at the bottom, an opening on the cup wall, and a small door (2) that can be opened and closed at the opening, so as to facilitate the removal of plant lateral roots for experimental observation. The nutrient cup (1) is placed through the eight circular openings on the perforated plastic plate (6), and the rectangular opening in the middle of the plastic plate (6) is used to observe the watering situation, and the small door (7) that can be opened and closed is provided at the opening. The seedling container is placed in the black light-proof plastic box (5) during the mycorrhizal seedling cultivation process to simulate the dark environment in the soil.

2. A mycorrhizal seedling raising container convenient for experimental observation according to claim 1, characterized in that: The seedling raising container is a set of containers composed of eight nutrition cups (1) of the same shape.

3. A mycorrhizal seedling raising container convenient for experimental observation according to claim 1, characterized in that: Each nutrient cup (1) is shaped like a truncated cone with a larger top and a smaller bottom, a cup mouth diameter of 10 cm, a cup rim width of 0.5 cm, a cup bottom diameter of 7 cm, a circular water leakage hole (4) with a diameter of 1 cm for convenient air permeability and water permeability, and a height of 20 cm, which is conducive to the extension of the root system of the mycorrhizal seedlings. A permeable cloth can be placed on the inner side of the bottom of each nutrient cup to prevent the matrix in the nutrient cup from leaking out.

4. The mycorrhizal seedling raising container convenient for experimental observation according to claim 1 is characterized in that: Each nutrient cup (1) is made of transparent plastic, which is convenient for observing the growth of mycorrhizal fungi. The cup wall has an opening in a rectangular shape with a size of 5 cm×6 cm. A small door (2) that can be opened and closed is provided at the opening, so that root segments can be taken from the opening for tests such as mycorrhizal infection rate determination. The material of the nutrient cup door (2) is the same as that of the nutrient cup (1).

5. The mycorrhizal seedling raising container convenient for experimental observation according to claim 1 is characterized in that: The perforated plastic plate (6) is made of black opaque plastic, has a size of 50 cm×50 cm, has 8 circular openings with a diameter of 10 cm and a rectangular opening with a size of 10 cm×10 cm, and the plastic plate door (7) is made of the same material as the perforated plastic plate (6).

6. The mycorrhizal seedling raising container convenient for experimental observation according to claim 1, characterized in that: The nutrient cup (1) is placed through the eight circular openings on the perforated plastic plate (6). The rectangular opening in the middle of the plastic plate (6) is used to observe the watering situation. A small door (7) with a size of 10 cm×10 cm that can be opened and closed is provided at the opening, so as to facilitate the observation of the watering situation at any time and prevent excessive watering.

7. The mycorrhizal seedling raising container convenient for experimental observation according to claim 1, characterized in that: During the cultivation of mycorrhizal seedlings, the seedling container is placed in a black light-proof plastic box (5) to simulate the dark environment in the soil. The dimensions of the black light-proof plastic box (5) are: 46 cm long, 46 cm wide, and 23 cm high. If it is necessary to observe the growth of mycorrhizae or to sample the plant roots during the seedling cultivation process, the nutrient cups need to be taken out of the plastic box. Each nutrient cup (1) can be taken out individually or the perforated plastic plate (6) can be lifted to take out 8 nutrient cups at the same time, so that the mycorrhizae growth can be observed at any time during the experiment.

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