Method for improving abundance of zooplankter in water body by using rotten liquid of pterocarya stenoptera leaves

Abstract

The invention relates to the technical field of ecological engineering, and discloses a method for improving abundance of zooplankton in water by using rotten liquid of pterocarya stenoptera leaves, which comprises the following steps: step 1, drying and grinding the pterocarya stenoptera leaves, and filtering by using a 325-mesh screen to obtain fine pterocarya stenoptera leaf powder suitable for microbial reaction; 2, the pterocarya stenoptera leaf powder is added into a water body microorganism stock solution with the concentration of 3 gC / L to 5 gC / L, the microorganism stock solution is water body raw water filtered through a 45-micron membrane, and mixing is formed. By adding the rotten liquid of the pterocarya stenoptera leaves into the water body, the abundance of zooplankton in the water body can be remarkably improved. The increase not only can promote the biomass and population diversity of other microorganisms in the water body, but also is helpful to improve the abundance and community diversity of zooplankton and form a more complex and abundant microbial ecological network, thereby improving the self-regulation ability of the water body and enhancing the ability of the water body to resist external pollution and ecological change.

Description

Technical Field

[0001] The invention relates to the technical field of ecological engineering, in particular to a method for increasing the abundance of zooplankton in a water body by utilizing rotten liquid of maple poplar leaves. Background Art

[0002] With the rapid development of the economy, the discharge of large amounts of industrial wastewater and domestic sewage has accelerated the eutrophication process of lakes and reservoirs. This process leads to an increase in the concentration of nutrients in the water, which in turn triggers the massive growth of phytoplankton and forms algal blooms. This not only seriously undermines the balance and stability of aquatic ecosystems, but also has a great impact on human production and life. During algal blooms, algal toxins produced by phytoplankton pose a threat to the human living environment. Therefore, controlling the growth of phytoplankton in water has become a research hotspot and an important task in the field of environmental science.

[0003] Food chain regulation technology is one of the effective methods for managing lake ecosystems. In lake ecosystems, various organisms such as aquatic plants, algae, zooplankton, fish and benthic animals restrict each other through predation, competition and other relationships, thus affecting the growth and reproduction of different organisms. The abundance of zooplankton is an important parameter for determining the density of lake phytoplankton and the risk of algal blooms. Studies have shown that planktonic crustaceans, as the main grazers of phytoplankton, can effectively inhibit the growth of phytoplankton and reduce the risk of algal blooms. Therefore, increasing the abundance of zooplankton has become an important goal for the restoration of lake ecosystems.

[0004] Currently, regulating zooplankton abundance mainly relies on fish regulation, which is achieved by adjusting the ratio of carnivorous fish to planktonic fish. However, in tropical and subtropical areas such as Guangzhou, due to the long breeding season of fish, the applicability of this method is limited and the effect is poor.

[0005] To this end, those skilled in the art have proposed a method for using the rotten liquid of maple poplar leaves to increase the abundance of zooplankton in water bodies to solve the above problems. Summary of the invention

[0006] In view of the shortcomings of the prior art, the present invention provides a method for increasing the abundance of zooplankton in water bodies by utilizing the rotten liquid of maple poplar leaves, thereby solving the problems raised by the above-mentioned background technology.

[0007] To achieve the above purpose, the present invention is implemented by the following technical scheme: a method for increasing the abundance of zooplankton in water bodies by using the rotten liquid of maple poplar leaves, comprising the following steps:

[0008] Step 1: Dry and crush the maple poplar leaves, and filter them using a 325-mesh sieve to obtain fine maple poplar leaf powder suitable for microbial reaction;

[0009] Step 2, adding the maple poplar leaf powder to a water body microbial stock solution at a concentration of 3-5 g C / L, wherein the microbial stock solution is water body raw water filtered through a 45 μm membrane, to form a mixture;

[0010] Step 3: placing the mixture in a plastic barrel, placing it in a dark room with a constant temperature for decay treatment, wrapping it with tin foil and covering it with a black curtain;

[0011] Step 4: The decay process lasts for 3-5 days, so that the abundance of flagellates in the decay fluid reaches 5-10 times that of the initial condition;

[0012] Step 5: Add the decomposing liquid to the target water body at a final concentration of 0.5-7 mg C / L to increase the abundance of zooplankton in the water body.

[0013] Preferably, the drying step of the maple poplar leaves in step 1 is achieved by natural air drying or low-temperature drying to maintain the stability of its nutritional components.

[0014] Preferably, the water microbial stock solution in step 2 includes but is not limited to bacteria, protozoa and other beneficial microorganisms to promote the growth of zooplankton.

[0015] Preferably, the decay treatment in step four increases dissolved oxygen by stirring, and the stirring method is achieved by injecting air into the liquid using an air pump to form bubbles, which stir the liquid during the rising process to increase dissolved oxygen.

[0016] Preferably, after the putrefying liquid is added to the water body in step five, the effectiveness of the method is evaluated by regularly monitoring the abundance and types of zooplankton in the water body.

[0017] Preferably, after the putrefaction liquid is added in step five, the concentration and frequency of the putrefaction liquid are regularly adjusted according to the actual conditions of the water body to ensure the best effect.

[0018] Preferably, the water body in step five is a freshwater lake, a river, a reservoir or an artificial water body, and the technical parameters can be adjusted according to specific environmental conditions.

[0019] Preferably, in the step 5, after the putrefying liquid is added, a biostimulant is added to enhance the balance of the water ecosystem, and the biostimulant includes limestone powder and magnesium sulfate.

[0020] Preferably, the temperature of the constant temperature darkroom in step three is 25-27°C.

[0021] The present invention provides a method for increasing the abundance of zooplankton in water bodies by using the rotten liquid of maple poplar leaves. It has the following beneficial effects:

[0022] 1. The present invention can significantly increase the abundance of zooplankton in the water body by adding the rotten liquid of maple poplar leaves to the water body. This increase not only contributes to the growth of zooplankton, but also promotes the reproduction of other microbial communities in the water body, forming a more complex and rich ecological network. As an important part of the food chain of water bodies, the increase in the number of zooplankton will provide sufficient food resources for fish, thereby promoting the health and stability of the entire ecosystem. In addition, the rich zooplankton population can improve the self-regulation ability of the water body, enhance its ability to resist external pollution and ecological changes, and ultimately achieve the sustainable development of the water ecosystem.

[0023] 2. The present invention utilizes the decaying liquid of maple poplar leaves to increase the abundance of zooplankton, which can not only increase the biomass in the water body, but also improve the physical and chemical properties of the water body by promoting microbial activity. The reproduction and metabolic activities of microorganisms will promote the decomposition of organic matter, reduce the concentration of harmful substances in the water body, and thus improve the water quality. At the same time, the increase in zooplankton will further reduce the eutrophication of the water body by feeding on microscopic algae and organic matter in the water, and maintain the cleanliness and transparency of the water body. Combined with the use of biostimulants, it can enhance the ecological balance of the water body, ensure the virtuous cycle of the water environment, promote the increase of biodiversity, and provide a strong guarantee for the healthy development of the water body. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a process flow chart of the present invention. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Embodiment 1:

[0027] Please refer to the attached Figure 1 The embodiment of the present invention provides a method for increasing the abundance of zooplankton in water bodies by using the rotten liquid of maple poplar leaves, comprising the following steps:

[0028] Step 1: Dry and crush the maple poplar leaves, and filter them using a 325-mesh sieve to obtain fine maple poplar leaf powder suitable for microbial reaction;

[0029] In step 1, the drying step of the maple poplar leaves is achieved by natural air drying or low-temperature drying to maintain the stability of its nutritional components.

[0030] Step 2: Adding maple poplar leaf powder at a concentration of 3-5 g C / L to a water body microbial stock solution, where the microbial stock solution is water body raw water filtered through a 45 μm membrane, to form a mixture;

[0031] The water microorganism stock solution in step 2 includes but is not limited to bacteria, protozoa and other beneficial microorganisms to promote the growth of zooplankton.

[0032] Step 3: Place the mixture in a plastic bucket and place it in a dark room with a constant temperature for decay, wrap it with tin foil and cover it with a black curtain;

[0033] The temperature of the constant temperature darkroom in step 3 is 27°C.

[0034] Step 4: The decay process lasts for 5 days, so that the abundance of flagellates in the decay fluid reaches 5-10 times that of the initial condition;

[0035] The decay treatment in step four increases dissolved oxygen by stirring. The stirring method is achieved by injecting air into the liquid using an air pump to form bubbles. The bubbles stir the liquid during the rising process, increasing the dissolved oxygen.

[0036] Step 5: Add the decomposed liquid to the target water body at a final concentration of 7 mg C / L to increase the abundance of zooplankton in the water body.

[0037] After the putrefaction liquid is added to the water body in step 5, the effectiveness of the method is evaluated by regularly monitoring the abundance and species of zooplankton in the water body. After the putrefaction liquid is added, the concentration and frequency of the putrefaction liquid are regularly adjusted according to the actual conditions of the water body to ensure the best effect, wherein the water body is a freshwater lake, river, reservoir or artificial water body, and the technical parameters can be adjusted according to the specific environmental conditions. After the putrefaction liquid is added, biostimulants are added to enhance the balance of the water ecosystem. The biostimulants include limestone powder and magnesium sulfate.

[0038] Specifically, after the rotten liquid is released, biostimulants are added to enhance the balance of the water ecosystem:

[0039] Limestone powder: regulates the pH value of water, improves water quality, and helps the survival of zooplankton.

[0040] Magnesium sulfate: replenishes the lack of trace elements in the water and promotes the growth of plants and zooplankton.

[0041] Embodiment 2:

[0042] A medium-sized ecosystem was established outdoors in a polyethylene plastic barrel with a volume of 200L (upper diameter × bottom diameter × height = 65cm × 50cm × 82cm). The experimental group and the control group were set up separately, with 4 parallels in each group, a total of 8 barrels, and 160L of shallow lake water in a university in the south was added to each barrel. The maple poplar leaves were collected, dried, crushed, filtered with a 325-mesh screen, and introduced into the water body. The microbial stock solution was rotted in the dark for 3-5 days, and the rotten liquid was placed in the experimental group on the first and tenth days at a final concentration of 5mgC / L. The control group did not receive any treatment. The experiment lasted for 26 days, and samples were taken on the 0th day and the last day of the experiment.

[0043] After the experiment, the average abundance of zooplankton in the control group was 180.82i nd.L (the average abundance of rotifers was 91.23i nd.L; the average abundance of Cyclops pygmysalis was 22.69i nd.L; the average abundance of nauplii was 55.85ind.L; the average abundance of Daphnia pulex was 10.15i nd.L, and the average abundance of Daphnia ceratitis was 0.9i nd.L); while the zooplankton in the experimental group was significantly greater than that in the control group. The average abundance of zooplankton in the experimental group was 512.81i nd.L (the average abundance of rotifers was 291.75i nd.L; the average abundance of Cyclops pygmysalis was 18.57i nd.L; the average abundance of nauplii was 98.25ind.L; the average abundance of Daphnia pulex was 60.75i nd.L, and the average abundance of Daphnia ceratitis was 60.50i nd.L).

[0044] Embodiment 3:

[0045] A medium-sized ecosystem was established outdoors in a polyethylene plastic barrel with a volume of 200L (upper diameter × bottom diameter × height = 65cm × 50cm × 82cm). The experimental group and the control group were set up separately, with 4 parallels in each group, a total of 8 barrels, and 160L of shallow lake water in a southern university was added to each barrel. The maple poplar leaves were collected, dried, crushed, filtered with a 325-mesh screen, and introduced into the water body microbial stock solution to rot in the dark for 3-5 days. The rotten liquid was placed in the experimental group on the first and tenth days at a final concentration of 15mgC / L. After the experiment, due to the introduction of a large amount of nutrients into the rotten liquid, although the heterotrophic flagellates increased and the proportion of blue-green algae in the water body increased significantly, resulting in an overall decline in food quality, the abundance of zooplankton, especially cladocerans, in the experimental group eventually showed a downward trend. The average abundance of zooplankton in the control group was 180.82i nd.L (the average abundance of rotifers was 91.23i nd.L; the average abundance of Cyclops in the warm zone was 22.69i nd.L; the average abundance of nauplii was 55.85i nd.L; the average abundance of Daphnia pulex was 10.15i nd.L, and the average abundance of Daphnia pulex was 0.9i nd.L), while the average abundance of zooplankton in the experimental group was 91.08i nd.L. The average abundance of rotifers was 45.43i nd.L; the average abundance of Cyclops in the warm zone was 15.67i nd.L; the average abundance of nauplii was 26.85i nd.L; the average abundance of Daphnia pulex was 3.14i nd.L, and Daphnia pulex was not detected.

[0046] Embodiment 4:

[0047] Fuxian Lake in the eastern part of Yunnan Province is an oligotrophic lake with clear and transparent water, very little sand content, very low content of various biological nutrients in the lake water, and low biological productivity. In recent years, the water quality of the lake area has shown an accelerated trend of eutrophication. Water samples were collected in the northern lake area of ​​Fuxian Lake (Chengjiang County), and were placed in 6 100L polyethylene plastic barrels, divided into 2 groups (experimental group and control group), with three parallels in each group. The maple poplar leaves purchased online were dried and crushed, filtered with a 325-mesh sieve, and introduced into the water body. The microbial stock solution was rotted in the dark for 3-5 days, and the rotten liquid was put into the experimental group at a final concentration of 5mgC / L on the first day of the experiment. The experiment lasted for 9 days, and sampling was carried out on the last day. Zooplankton detected elephant fleas, net fleas and phytoplankton. The control group of elephant fleas was 6.67i nd / L, and the experimental group was 161.33i nd / L; the control group of net fleas was 0.67ind / L, and the experimental group was 5.33i nd / L; the control group of Daphnia pulex was 0.67i nd / L, and the experimental group was 2.67i nd / L. The experimental and control groups of Daphnia pulex were 0.67i nd / L. After the corn stalk rot liquid was added, the abundance of total zooplankton in the water body increased significantly compared with the control group.

[0048] Embodiment 5:

[0049] A shallow lake in a university in the south was selected as the experimental lake. The average length of the lake is 148.5m, the width is 31.2m, the total area is 4638m2, the average depth is 0.7cm, and it is closed on all sides. The main sources of lake water are surface rainwater and some domestic sewage. The lake had a serious eutrophication problem earlier, and ecological restoration work was carried out at the end of 2013. The lake after restoration has significantly improved in terms of water transparency and water quality. At this time, omnivorous small fish were found to grow in the lake water, the abundance of zooplankton decreased, and finally phytoplankton grew in large quantities, and its average chlorophyll content increased from 16.95μg / L when it was just repaired to 231.91μg / L. The rotten liquid was put into the lake water at a final concentration of 0.5mgC / L. Samples were taken at the beginning and 7 days after the release. At the beginning, the zooplankton detected included Daphnia pygmaeus (1.25i nd / L), Daphnia pulex (0.75i nd / L) and Cyclops pygmaeus (6.50i nd / L), and the total zooplankton abundance was 8.5i nd / L; at the end of the experiment, the zooplankton detected included Daphnia pygmaeus (2.50i nd / L), Daphnia pulex (7.50i nd / L), Daphnia pulex (10.50i nd / L) and Cyclops pygmaeus (6.60i nd / L), and the total zooplankton abundance increased significantly to 27.10i nd / L.

[0050] The following table 1 is the experimental data of Example 2 to Example 5:

[0051]

[0052] Table 1

[0053] Conclusion: The decomposition liquid of maple poplar leaves can effectively increase the abundance of zooplankton in water bodies, but the choice of concentration is crucial. The right amount of decomposition liquid can promote the growth of zooplankton, while too high a concentration may lead to a decrease in food quality, thus affecting abundance. This finding provides a theoretical basis for ecological water management and zooplankton proliferation.

[0054] In summary: By adding the rotten liquid of maple poplar leaves to the water body, the abundance of zooplankton in the water body can be significantly increased. This increase not only helps the growth of zooplankton, but also promotes the reproduction of other microbial communities in the water body, forming a more complex and rich ecological network. As an important part of the food chain of water bodies, the increase in the number of zooplankton will provide sufficient food resources for fish, thereby promoting the health and stability of the entire ecosystem. In addition, the rich zooplankton population can improve the self-regulation ability of the water body, enhance its ability to resist external pollution and ecological changes, and ultimately achieve the sustainable development of the water ecosystem.

[0055] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for increasing the abundance of zooplankton in water bodies by using the rotten liquid of maple poplar leaves, characterized in that: The following steps are involved: Step 1: Dry and crush the maple poplar leaves, and filter them using a 325-mesh sieve to obtain fine maple poplar leaf powder suitable for microbial reaction; Step 2, adding the maple poplar leaf powder to a water body microbial stock solution at a concentration of 3-5 g C / L, wherein the microbial stock solution is water body raw water filtered through a 45 μm membrane, to form a mixture; Step 3: placing the mixture in a plastic barrel, placing it in a dark room with a constant temperature for decay treatment, wrapping it with tin foil and covering it with a black curtain; Step 4: The decay process lasts for 3-5 days, so that the abundance of flagellates in the decay fluid reaches 5-10 times that of the initial condition; Step 5: Add the decomposing liquid to the target water body at a final concentration of 0.5-7 mg C / L to increase the abundance of zooplankton in the water body.

2. The method of using rotten maple poplar leaf liquid to increase the abundance of zooplankton in water bodies according to claim 1, characterized in that: In the step 1, the drying step of the maple poplar leaves is achieved by natural air drying or low-temperature drying to maintain the stability of its nutritional components.

3. The method of using rotten maple poplar leaf liquid to increase the abundance of zooplankton in water bodies according to claim 1, characterized in that: The water microorganism stock solution in step 2 includes but is not limited to bacteria, protozoa and other beneficial microorganisms to promote the growth of zooplankton.

4. The method of using rotten maple poplar leaf liquid to increase the abundance of zooplankton in water bodies according to claim 1, characterized in that: The decay treatment in step four increases dissolved oxygen by stirring, and the stirring method is to inject air into the liquid using an air pump to form bubbles. The bubbles will stir the liquid during the rising process to increase the dissolved oxygen.

5. The method of using rotten maple poplar leaf liquid to increase the abundance of zooplankton in water bodies according to claim 1, characterized in that: After the putrefaction liquid is released into the water body in step 5, the effectiveness of the method is evaluated by regularly monitoring the abundance and types of zooplankton in the water body.

6. The method of using rotten maple poplar leaf liquid to increase the abundance of zooplankton in water bodies according to claim 1, characterized in that: After the decomposing liquid is added in step 5, the concentration and frequency of the decomposing liquid are regularly adjusted according to the actual conditions of the water body to ensure the best effect.

7. The method of using rotten maple poplar leaf liquid to increase the abundance of zooplankton in water bodies according to claim 1, characterized in that: The water body in step 5 is a freshwater lake, river, reservoir or artificial water body, and the technical parameters can be adjusted according to specific environmental conditions.

8. The method of using rotten maple poplar leaf liquid to increase the abundance of zooplankton in water bodies according to claim 1, characterized in that: In the step 5, after the rotten liquid is added, a biostimulant is added to enhance the balance of the water ecosystem, and the biostimulant includes organic fertilizer, limestone powder, and magnesium sulfate.

9. The method of using rotten maple poplar leaf liquid to increase the abundance of zooplankton in water bodies according to claim 1, characterized in that: The temperature of the constant temperature darkroom in step 3 is 25-27°C.

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