Ore-containing salt lake brine microorganism sampling device and sampling method

Abstract

The invention relates to the technical field of microorganism sampling equipment, in particular to an ore-containing salt lake brine microorganism sampling device and method.The ore-containing salt lake brine microorganism sampling device comprises a balance weight floating platform, and through cooperation of a laser ranging mechanism, a PLC, a first electromagnetic valve, a second electromagnetic valve and an air pump, the sampling depth can be adjusted according to the actual sampling depth requirement; sampling distance parameters are flexibly set through the PLC according to actual sampling requirements, the winding mechanism is controlled to work through the PLC, the winding mechanism drives the sampling head to be lowered or pulled back, the distance between the sampling head and the bottom plate is detected through the laser ranging mechanism, and when the actual distance detected by the laser ranging mechanism reaches a set value, the sampling head is driven to move back and forth. The first electromagnetic valve and the second electromagnetic valve on the to-be-sampled barrel are started through the PLC, the air pump is started, a sample enters from the sampling head through an air pumping mode of the air pump, the operation is simple, the use is convenient and fast, and meanwhile, the sampling flexibility of samples with different depths is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial sampling equipment, and in particular relates to a device and a method for sampling microbial sampling of mineral-containing salt lake brine. Background Art

[0002] With the construction of world-class salt lake industrial bases and the rise in lithium carbonate prices, the salt lake lithium extraction industry is booming. Salt lakes are important mineral resource bases that are not only rich in lithium, potassium, magnesium and other minerals, but also a unique microbial ecosystem.

[0003] Brine refers to liquid minerals with a salt content greater than 5%. It can be divided into surface brine (or lake brine) and underground brine according to its accumulation location. Salt lake brine usually has the characteristics of high salinity, high density and strong corrosiveness. Its density can reach 1.200-1.300g / m 3 Brine contains a variety of ions, such as potassium, magnesium, lithium, etc., and the concentrations and proportions of these ions may vary significantly at different depths.

[0004] In order to conduct a comprehensive study and effective development of salt lake brine, it is necessary to accurately sample brine at different depths through a sampler. Generally speaking, the sampling depth of underground brine ranges from 0 to 50m, and the sampling volume should usually reach 900 to 1400mL each time to meet the needs of subsequent analysis. However, due to the huge area of ​​the salt lake, the complex composition of the brine, and the presence of mineral deposition and other factors, accurate stratified sampling faces many challenges.

[0005] In recent years, with the deepening of understanding of salt lake ecosystems, researchers have discovered that there are rich and diverse microbial communities in salt lake brines. These microorganisms not only play an important role in salt lake ecosystems, but more importantly, they play an important guiding role in mineralization principles and mineral exploration. Microorganisms play a key role in the formation, transformation and aggregation of minerals. By studying the structure and function of microbial communities in brines at different depths, we can provide important clues for understanding the formation mechanism of salt lake mineral resources. In addition, certain specific microbial communities may be related to the enrichment of specific mineral resources, so the study of these microorganisms can provide new ideas and methods for mineral exploration. Given the importance of salt lake brine microbial research to the development of mineral resources, it is particularly important to accurately sample and conduct in-depth research on them.

[0006] However, the sampling of salt lake brine microorganisms faces unique challenges. First, the precise sampling depth needs to be ensured to obtain microbial samples from different water layers, so as to accurately analyze the changes in microbial communities with depth and their relationship with the distribution of mineral resources. Secondly, cross-contamination of samples from different layers should be avoided during the sampling process to ensure the accuracy and reliability of microbial data. In addition, the effects of the high salinity and corrosiveness of brine on sampling equipment need to be considered to ensure that the sampling equipment can work stably in harsh environments for a long time.

[0007] At present, there are some microbial sampling devices that can collect samples at different depths. For example, patent application number 202211244505.3 discloses a sample sampling device for underwater microbial detection, including a sample sampling device for underwater microbial detection, including a counterweight floating platform, the bottom of the counterweight floating platform is hinged with a sampling tube holder through a torsion spring shaft, and the sampling tube holder is evenly arranged along the circumferential direction of the counterweight floating platform, and the outer side of the sampling tube holder is provided with an outer ring plate that matches the limit position, and the outer ring plate moves downward in the vertical direction. All sampling tube holders are rotated relative to the counterweight floating platform through torsion springs, and the sampling tube inside the sampling tube holder is supported on the top of the outer ring plate and arranged in a multi-angle high and low dislocation. Through the setting of the above structure, the outer ring plate in the invention can realize the protection of the outer wall of the sampling tube, and at the same time, the sampling tubes in different directions can be arranged in a high and low dislocation, and samples are collected in different directions and at different depths under the water surface, so as to obtain different samples to improve data detection.

[0008] However, this technology has the following limitations:

[0009] Insufficient flexibility in sampling depth: Although the sampling tubes can be arranged at multiple angles and heights, the position and angle of each sampling tube are pre-set and difficult to adjust according to actual needs.

[0010] Fixed sampling spacing: Limited by the outer ring plate and torsion spring, the distance between the sampling tubes is difficult to adjust flexibly and cannot meet different sampling requirements.

[0011] Complex operation: The existing device has a complex structure and is inconvenient to operate, which is not conducive to rapid and efficient sampling in a salt lake environment.

[0012] Microbiological Sample Integrity: There is no guarantee that microbiological samples will not be damaged or contaminated during the sampling process.

[0013] Therefore, we need to propose a mineral-containing salt lake brine microbial sampling device and sampling method to solve the above-mentioned problems, so that it can improve the flexibility of sampling samples at different depths. Summary of the invention

[0014] In view of the above problems, the present invention provides a device for sampling microorganisms of mineral-bearing salt lake brine, comprising a weighted floating platform, a base is fixed at the lower end of the weighted floating platform, a bottom plate is fixed at the lower end of the base, a plurality of sampling barrels are installed at equal distances around the base, each sampling barrel is installed with a discharge faucet for draining liquid, an air pump and a PLC controller are installed inside the weighted floating platform, a plurality of the sampling barrels are connected to the air extraction end of the air pump through a first branch pipe, and a first solenoid valve is installed on the first branch pipe, a winding mechanism is installed on the base, a sampling tube is wound on the winding mechanism, and one end of the sampling tube is connected to a sampling head capable of preliminarily filtering the salt lake brine;

[0015] The other end of the sampling tube is connected to the lower ends of multiple sampling barrels through a connecting mechanism, and the connecting mechanism is provided with second solenoid valves corresponding to the number of the multiple sampling barrels and controlling the samples to enter the sampling barrels. A laser ranging mechanism is detachably installed on the lower surface of the bottom plate, and a reflecting plate is installed on the upper end of the sampling head. The reflecting plate is used to reflect the laser signal emitted by the laser ranging mechanism. The PLC controller is electrically connected to the multiple first solenoid valves, the second solenoid valve, the laser ranging mechanism, the air pump and the winding mechanism, respectively, and the first solenoid valve and the second solenoid valve connected to each of the sampling barrels work synchronously. One end of the PLC controller is electrically connected to a battery.

[0016] Furthermore, when setting the sampling distance, the PLC controller sets the same or different sampling distances for each sampling bucket.

[0017] Furthermore, the sampling barrel comprises a barrel body, a piston block matching the inner diameter of the barrel body is slidably arranged inside the barrel body, and label paper is arranged on the outer wall of the sampling barrel.

[0018] Furthermore, the sampling head includes an upper cover and a lower cover made of stainless steel, which are connected by screws after being plugged in, a filter plate is detachably installed between the upper cover and the lower cover, sampling holes are opened around the lower cover, the reflector is fixed on the upper surface of the upper cover, and the lower end of the sampling tube is connected to the upper end of the upper cover.

[0019] Furthermore, a third solenoid valve is installed on the sampling tube located at the upper cover, the third solenoid valve is electrically connected to the PLC controller, and the filter plate is configured as a grid plate or a non-woven filter mesh plate.

[0020] Furthermore, the bottom plate and the base are combined to form a cavity, and the connecting mechanism is located inside the cavity. The connecting mechanism includes a second branch pipe connected to the lower end of each sampling barrel and a connecting head connected to the winding mechanism. A plurality of the second branch pipes are distributed at equal angles on the periphery of the connecting head, one end of the connecting head is connected to the sampling tube on the winding mechanism, and the second solenoid valve is located on the second branch pipe near the connecting head.

[0021] Furthermore, a groove connected to the cavity is provided on the base, and the winding mechanism includes a motor installed on one side of the groove and a hollow winding roller rotatably installed inside the groove, the other end of the sampling tube is inserted into the winding roller and led out from the end of the winding roller to be connected to the connector, and transmission gears are installed on the outer wall of one end of the winding roller and the output shaft of the motor, and the two transmission gears are meshed.

[0022] Furthermore, an installation cavity for installing an air pump is provided inside the counterweight floating platform, an exhaust hole is provided at the upper end of the installation cavity, and the battery and the PLC controller are both located inside the installation groove.

[0023] Furthermore, the laser ranging mechanism includes a laser ranging sensor, the outer part of which is sleeved with a protective cover, a transparent plate is provided on one side of the protective cover located at the emitting end of the laser ranging sensor, a cover plate is covered on the side of the protective cover opposite to the transparent plate, the cover plate is in contact with the lower surface of the base plate, and the protective cover is detachably connected to the base plate by bolts.

[0024] Furthermore, bolts are installed on all four edges of the side of the protective cover opposite to the transparent plate. The bolts include studs and nuts. One end of the stud passes through the cover plate and the bottom plate and is threadedly connected to the nut. A sealing ring is provided between the cover plate and the protective cover.

[0025] Based on the above-described device for sampling microorganisms in brine of a mineral-containing salt lake, the present invention further provides a method for sampling microorganisms in brine of a mineral-containing salt lake, comprising the following steps:

[0026] S1. Setting the sampling distance parameters for each sampling bucket through the PLC controller;

[0027] S2, control the winding mechanism to work through the PLC controller, so that the winding mechanism drives the sampling head to be lowered;

[0028] S3, the distance between the sampling head and the bottom plate is monitored in real time by a laser distance measuring mechanism. When the distance between the sampling head and the bottom plate reaches the set sampling distance, the air pump starts working, and the first solenoid valve and the second solenoid valve connected to the sampling barrel are controlled by the PLC controller to start sampling;

[0029] S4, after the sampling is completed, the PLC controller is used to control the winding mechanism to drive the sampling head to pull back;

[0030] The sampling distance is set to be the same or different for each sampling barrel in turn during the setting. During sampling, when the sampling distance of each sampling barrel is set to be the same, after the distance the sampling head is lowered reaches the set distance, the first solenoid valve and the second solenoid valve on half of the sampling barrels are started first, and the air pump is started to sample half of the sampling barrels. After the sampling of half of the sampling barrels is completed, the half of the sampling barrels are used to store the residual water in the sampling tubes, and then the other half of the sampling barrels are started in the same way, and these sampling barrels are used to collect the sampled samples.

[0031] When the sampling distances of multiple sampling barrels are different, when the sampling head is lowered to the sampling distance set for the first sampling barrel, first start the first solenoid valve and the second solenoid valve corresponding to the sampling barrel adjacent to the first sampling barrel, then start the air pump to extract air and sample to transport the residual liquid in the sampling tube to the sampling barrel adjacent to the first sampling barrel, then close the first solenoid valve and the second solenoid valve corresponding to the sampling barrel adjacent to the first sampling barrel, and open the first solenoid valve and the second solenoid valve corresponding to the first sampling barrel at the same time to take samples, and close the first solenoid valve and the second solenoid valve corresponding to the first sampling barrel after the sampling is completed; the winding mechanism continues to lower the sampling head, and when the sampling head is lowered to the sampling distance set for the second sampling barrel, the second sampling barrel is sampled using the same sampling method as the first sampling barrel, and this is repeated until all sampling barrels are sampled.

[0032] The beneficial effects of the present invention are:

[0033] 1. The present invention can set the sampling distance parameters through the PLC controller according to the actual sampling depth requirements through the cooperation of the laser distance measuring mechanism, the PLC controller, the first solenoid valve, the second solenoid valve and the air pump, and control the winding mechanism to work through the PLC controller, so that the winding mechanism drives the sampling head to be lowered or pulled back, and uses the laser distance measuring mechanism to detect the distance between the sampling head and the bottom plate. When the actual distance detected by the laser distance measuring mechanism reaches the set value, the first solenoid valve and the second solenoid valve on the barrel to be sampled are opened through the PLC controller, and the air pump is started. The sample enters from the sampling head through the air pump, and then enters the interior of the sampling barrel through the sampling tube and the second solenoid valve. After the sampling is completed, the first solenoid valve and the second solenoid valve connected to the sampling barrel are closed to complete the sampling of the sample. In this way, the sampling distance can be flexibly set according to the actual sampling requirements, the operation is simple, the use is convenient, and the flexibility of sampling samples at different depths is improved.

[0034] 2. The sampling device provided by the present invention is equipped with multiple independently controlled sampling barrels, which realizes a highly flexible sampling strategy. In the multi-point synchronous sampling mode, the system can start multiple sampling barrels at the same depth at the same time to collect samples from different horizontal positions, which is extremely useful for studying the horizontal distribution characteristics of a specific depth. In the segmented sampling mode, the device can sample one by one at different depths. By precisely controlling the descent and recovery of the sampling head, combined with the independent control of each sampling barrel, continuous sampling from the surface to the deep layer is achieved, providing an ideal solution for studying vertical distribution characteristics. This flexible sampling method not only significantly improves the sampling efficiency, but also obtains more comprehensive and representative sample data, providing strong support for the spatial distribution of salt lake microbial communities and mineralization theory research.

[0035] 3. The present invention has adopted multiple protection measures to ensure the purity of the sample and the reliability of the data. First, the independent sampling barrel system is equipped with a special solenoid valve to effectively isolate different samples. Secondly, the transition barrel is designed to remove residues in the sampling tube, significantly reducing the risk of contamination between samples of different depths. Through the precise control of the solenoid valve by the PLC controller, it is ensured that only the valve of the target sampling barrel is opened during the sampling process, further reducing the possibility of cross contamination. In addition, the detachable design of the sampling head facilitates regular cleaning, and the one-way flow design reduces the risk of backflow, both of which reduce the risk of contaminant accumulation. The combined application of these measures greatly improves the purity and reliability of samples during continuous sampling.

[0036] 4. The present invention provides a highly flexible, accurate, efficient and adaptable salt lake brine microbial sampling device and method through innovative design. Through the PLC controller, laser ranging mechanism and adjustable winding mechanism, precise control of sampling depth and fully automated sampling process are achieved, greatly improving the accuracy and efficiency of sampling. The label paper design outside the sampling barrel further improves the efficiency of sample management. These innovations not only significantly improve the reliability of sample data, provide a more accurate scientific basis for salt lake mineralization principle research and prospecting, but also achieve significant economic benefits by improving sampling efficiency and reducing manpower and time costs. The present invention solves many problems in the prior art, provides strong technical support for the in-depth research and efficient development of salt lake resources, promotes research and application progress in related fields, and is of great significance to improving the scientific level of salt lake resource exploration and utilization.

[0037] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures pointed out in the description, claims and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0039] Figure 1 A schematic diagram of the structure of an embodiment of the present invention is shown;

[0040] Figure 2 A schematic cross-sectional structure diagram according to an embodiment of the present invention is shown;

[0041] Figure 3 The embodiment of the present invention is shown Figure 2 The enlarged structural diagram is shown in the figure;

[0042] Figure 4 A schematic diagram of a bottom-up structure according to an embodiment of the present invention is shown;

[0043] Figure 5 A schematic diagram of the structure of a connecting mechanism according to an embodiment of the present invention is shown;

[0044] Figure 6 A schematic diagram of the structure of a sampling barrel according to an embodiment of the present invention is shown;

[0045] Figure 7 A schematic diagram of the structure of a sampling head according to an embodiment of the present invention is shown;

[0046] Figure 8 A schematic structural diagram of a laser ranging mechanism according to an embodiment of the present invention is shown.

[0047] In the figure: 1, counterweighted floating platform; 2, base; 3, sampling barrel; 301, barrel body; 302, piston block; 4, sampling head; 401, lower cover; 402, upper cover; 403, filter plate; 404, protrusion; 405, injection hole; 5, discharge tap; 6, connecting pipe; 7, first solenoid valve; 8, first branch pipe; 9, sampling tube; 10, laser distance measuring mechanism; 1001, protective cover; 1002, transparent plate; 10 03. Laser ranging sensor; 1004. Sealing ring; 1005. Cover plate; 1006. Stud; 1007. Nut; 11. Air pump; 12. Second branch pipe; 13. Connector; 14. Second solenoid valve; 15. Cavity; 16. Winding roller; 17. Motor; 18. Transmission gear; 19. Bottom plate; 20. Reflector; 21. Exhaust hole; 22. Installation cavity; 23. Label paper; 24. Third solenoid valve. DETAILED DESCRIPTION

[0048] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] The embodiment of the present invention provides a device for sampling brine microorganisms in a mineral-containing salt lake, such as Figure 1-Figure 8 As shown, it includes a counterweight floating platform 1, a base 2 is fixed at the lower end of the counterweight floating platform 1, a bottom plate 19 is fixed at the lower end of the base 2, a cavity 15 is formed between the bottom plate 19 and the base 2, a plurality of sampling barrels 3 are installed at equal distances around the base 2, each sampling barrel 3 is installed with a discharge faucet 5 for draining liquid, an air pump 11 and a PLC controller are installed inside the counterweight floating platform 1, a plurality of the sampling barrels 3 are connected to the air extraction end of the air pump 11 through a first branch pipe 8, and a first solenoid valve 7 is installed on the first branch pipe 8, a winding mechanism is installed on the base 2, a sampling tube 9 is wound on the winding mechanism, one end of the sampling tube 9 is connected to a sampling head 4 capable of preliminarily filtering salt lake brine, and the The other end of the sampling tube 9 is connected to the lower ends of the plurality of sampling barrels 3 through a connecting mechanism, and the connecting mechanism is provided with second solenoid valves 14 corresponding to the number of the plurality of sampling barrels 3 and controlling the samples to enter the sampling barrels 3. The lower surface of the bottom plate 19 is detachably mounted with a laser ranging mechanism 10, and the upper end of the sampling head 4 is mounted with a reflecting plate 20, and the reflecting plate 20 is used to reflect the laser signal emitted by the laser ranging mechanism 10. The PLC controller is electrically connected to the plurality of first solenoid valves 7, the second solenoid valve 14, the laser ranging mechanism 10, the air pump 11 and the winding mechanism, respectively, and the first solenoid valve 7 and the second solenoid valve 14 connected to each of the sampling barrels 3 work synchronously, and one end of the PLC controller is electrically connected to a battery.

[0050] When in use, the sampling distance parameters are set through the PLC controller according to the actual sampling depth requirements, and the winding mechanism is controlled by the PLC controller to drive the sampling head 4 to be lowered or pulled back, and the laser distance measuring mechanism 10 is used to detect the distance between the sampling head 4 and the bottom plate 19. When the actual distance detected by the laser distance measuring mechanism 10 reaches the set value, the first solenoid valve 7 and the second solenoid valve 14 on the sampling barrel 3 are opened by the PLC controller, and the air pump 11 is started. The sample enters from the sampling head 4 by means of air extraction by the air pump 11, and then enters the interior of the sampling barrel 3 through the sampling tube 9 and the second solenoid valve 14. After the sampling is completed, the first solenoid valve 7 and the second solenoid valve 14 connected to the sampling barrel 3 are closed to complete the sampling of the sample. In this way, the sampling distance can be flexibly set according to the actual sampling requirements, thereby improving the flexibility of sampling samples at different depths.

[0051] When the PLC controller sets the sampling distance, the same or different sampling distances are set for each sampling barrel 3. When the sampling distance of each sampling barrel 3 is set to be the same, when the distance of the sampling head 4 lowered reaches the set distance, the first solenoid valve 7 and the second solenoid valve 14 on half of the sampling barrels 3 are started first, and the air pump 11 is started to sample half of the sampling barrels 3. After the sampling of half of the sampling barrels 3 is completed, the half of the sampling barrels 3 are used to store the residual water in the sampling tube 9, which can be named as transition barrels for distinction, and then the same method is used to store the residual water in the sampling tube 9. The other half of the sampling barrels 3 are started in the same way, and these sampling barrels 3 are used to collect sampling samples, so as to realize simultaneous sampling of multiple sampling barrels 3; when the sampling distances of multiple sampling barrels 3 are different, such as the sampling distances are 5m, 10m, 15m and 20m respectively, the multiple sampling barrels 3 are numbered according to the sampling distances, such as the sampling distances of 5m samples, 10m samples, 15m samples and 20m samples, and an empty sampling barrel 3 is spaced between each number and named as a transition sample. When the distance of the sampling head 4 reaching 5m, start one sampling barrel first. The first solenoid valve 7 and the second solenoid valve 14 corresponding to the transition sample are opened, and then the air pump 11 is started for air extraction sampling to transport the residual liquid in the sampling tube 9 to the sampling barrel 3 marked with the transition sample, and then the first solenoid valve 7 and the second solenoid valve 14 corresponding to the transition sample are closed, and the first solenoid valve 7 and the second solenoid valve 14 corresponding to the 5m sample are opened at the same time. After the sampling is completed, the first solenoid valve 7 and the second solenoid valve 14 corresponding to the 5m sample are closed; the winding mechanism continues to lower the sampling head 4. When the sampling head 4 is lowered to a distance of 10m, the first solenoid valve 7 and the second solenoid valve 14 corresponding to another transition sample are started first, and then the air pump 11 is started for air extraction sampling to transport the residual liquid in the sampling tube 9 to the sampling barrel 3 marked with the transition sample, and then the first solenoid valve 7 and the second solenoid valve 14 corresponding to the transition sample are closed, and the first solenoid valve 7 and the second solenoid valve 14 corresponding to the 10m sample are opened at the same time. After the sampling is completed, the first solenoid valve 7 and the second solenoid valve 14 corresponding to the 10m sample are closed, and the above operation is continued until all the sampling barrels 3 are sampled. The operation is simple and convenient to use.

[0052] When transferring the sample in the sampling barrel 3 , the sample in the sampling barrel 3 is transferred by opening the discharge tap 5 on the sampling barrel 3 . The operation is simple, and the convenience of transferring the sample in the sampling barrel 3 is improved.

[0053] like Figure 6As shown, the sampling barrel 3 includes a barrel body 301, and a piston block 302 matching the inner diameter of the barrel body 301 is slidably arranged inside the barrel body 301. When the first solenoid valve 7 and the second solenoid valve 14 are opened, the air pump 11 drives the piston block 302 to move upward by pumping air. At the same time, the movement of the piston block 302 causes the pressure inside the barrel body 301 to change, so that the liquid in the sampling tube 9 enters the interior of the barrel body 301. At the same time, the piston block 302 can be used to block the liquid and gas, thereby facilitating gas-liquid separation.

[0054] The outer wall of the sampling barrel 3 is provided with a label paper 23, and the label paper 23 is located above the discharge faucet 5. The setting of the label paper 23 facilitates personnel to quickly identify the samples in each sampling barrel 3 when sampling, and also facilitates personnel to name each sampling barrel 3 according to the label paper 23, so as to automate the sampling operation of samples of different depths.

[0055] like Figure 7 As shown, the sampling head 4 includes an upper cover 402 and a lower cover 401 made of stainless steel. The upper cover 402 and the lower cover 401 are connected by screws after being plugged in. A filter plate 403 is detachably installed between the upper cover 402 and the lower cover 401. The lower cover 401 is provided with injection holes 405 around it. The reflector 20 is fixed on the upper surface of the upper cover 402. The lower end of the sampling tube 9 is connected to the upper end of the upper cover 402. The upper cover 402 and the lower cover 401 are detachably connected. The upper cover 402 and the lower cover 401 are connected to each other so as to be separated from each other regularly, so as to replace or clean the filter plate 403 inside regularly. The sample can be easily introduced into the interior of the sampling head 4 through the sampling hole 405 and transmitted through the sampling tube 9. The stainless steel material of the upper cover 402 and the lower cover 401 is used to increase the weight of the sampling head 4, reduce the floating or swaying phenomenon of the sampling head 4 in the salt lake, and enable the sampling head 4 to be stably suspended vertically below the bottom plate 19 to complete the laser ranging operation.

[0056] A third solenoid valve 24 is installed on the sampling tube 9 located at the upper cover 402. The third solenoid valve 24 is electrically connected to the PLC controller. Due to the setting of the third solenoid valve 24, when the sampling tube 9 is lowered, the third solenoid valve 24 is in a closed state to prevent liquid from entering the interior of the sampling tube 9. When the sampling tube 9 is transmitting samples, the third solenoid valve 24 is in an open state to facilitate the transmission of samples.

[0057] The inner walls on the opposite sides of the upper cover 402 and the lower cover 401 are both provided with protrusions 404 for positioning the filter plate 403, and the distance between the protrusions 404 of the upper cover 402 and the protrusions 404 of the lower cover 401 is the same as the thickness of the filter plate 403, which is convenient for clamping and fixing the filter plate 403. The filter plate 403 is configured as a grid plate or a non-woven filter mesh plate, and the particulate matter in the salt lake brine is preliminarily filtered through the filter plate 403 to prevent the particulate matter from entering the sampling tube 9 and causing blockage.

[0058] The connecting mechanism is located inside the cavity 15, and includes a second branch pipe 12 connected to the lower end of each sampling barrel 3 and a connecting head 13 connected to the winding mechanism. A plurality of second branch pipes 12 are distributed at equal angles on the periphery of the connecting head 13, and one end of the connecting head 13 is connected to the sampling tube 9 on the winding mechanism. The second solenoid valve 14 is located on the second branch pipe 12 near the connecting head 13. The connecting head 13 facilitates the connection of a plurality of second branch pipes 12 with the sampling tube 9, so as to facilitate the directional transmission of samples by controlling the opening of different second solenoid valves 14, thereby improving the convenience of sample transmission.

[0059] The base 2 is provided with a groove connected to the cavity 15, and the winding mechanism includes a motor 17 installed on one side of the groove and a hollow winding roller 16 rotatably installed inside the groove. The other end of the sampling tube 9 is inserted into the winding roller 16 and led out from the end of the winding roller 16 to communicate with the connector 13. This connection method facilitates the lowering or pulling back of the sampling tube 9 on the winding roller 16 when the winding roller 16 rotates. A transmission gear 18 is installed on the outer wall of one end of the winding roller 16 and the output shaft of the motor 17, and the two transmission gears 18 are meshed. When winding, the motor 17 uses the two meshed transmission gears 18 to drive the winding roller 16 to rotate forward or reverse, so as to lower or pull back the sampling tube 9 wound on the winding roller 16.

[0060] An installation cavity 22 for installing the air pump 11 is provided inside the counterweight floating platform 1. A connecting pipe 6 connected to the suction end of the air pump 11 is provided at the upper end of the installation cavity 22. Multiple first branch pipes 8 are connected at equal angles around the connecting pipe 6. An exhaust hole 21 is provided at the upper end of the installation cavity 22. The battery and PLC controller (not shown in the figure) are both located inside the installation slot. The battery, PLC controller and air pump 11 are powered to ensure the normal operation of the air pump 11 and the PLC controller. The PLC controller includes a touch screen (not shown in the figure). The touch screen is located on the upper surface of the counterweight floating platform 1, so that the parameters of the sampling distance can be set through the touch screen.

[0061] like Figure 8As shown, the laser ranging mechanism 10 includes a laser ranging sensor 1003, the outer part of which is sleeved with a protective cover 1001, a transparent plate 1002 is provided on one side of the protective cover 1001 located at the emitting end of the laser ranging sensor 1003, a cover plate 1005 is covered on the side of the protective cover 1001 opposite to the transparent plate 1002, the cover plate 1005 is in contact with the lower surface of the bottom plate 19, the protective cover 1001 is detachably connected to the bottom plate 19 by bolts, and the laser ranging sensor 1003 is protected by the protective cover 1001 to prevent the laser ranging sensor 1003 from being directly in contact with water and being damaged.

[0062] Bolts are installed on the edges of the side of the protective cover 1001 opposite to the transparent plate 1002, and the bolts include studs 1006 and nuts 1007. One end of the stud 1006 passes through the cover plate 1005 and the bottom plate 19 and is threadedly connected to the nut 1007. A sealing ring 1004 is provided between the cover plate 1005 and the protective cover 1001. The sealing ring 1004 is used to increase the sealing between the protective cover 1001 and the cover plate 1005. The threaded cooperation between the nut 1007 and the stud 1006 makes the protective cover 1001 detachable, thereby improving the convenience of installation and maintenance of the laser ranging mechanism 10.

[0063] The laser ranging sensor 1003 emits a short laser pulse to the reflector 20. After the laser is reflected by the reflector 20, part of the scattered light returns to the receiver and records the time required for the pulse to be emitted and returned. By calculating the round-trip time of the laser light and multiplying it by the speed of light, the distance can be accurately calculated, that is, the target distance can be measured. The laser sensor uses common knowledge technology in the field and will not be described in detail here.

[0064] Based on the above-described device for sampling microorganisms in brine of a mineral-containing salt lake, the present invention further provides a method for sampling microorganisms in brine of a mineral-containing salt lake, comprising the following steps:

[0065] S1, setting the sampling distance parameters for each sampling bucket 3 through the PLC controller;

[0066] S2, controlling the winding mechanism to work through the PLC controller, so that the winding mechanism drives the sampling head 4 to be lowered;

[0067] S3, the distance between the sampling head 4 and the bottom plate 19 is monitored in real time by the laser distance measuring mechanism 10. When the distance between the sampling head 4 and the bottom plate 19 reaches the set sampling distance, the air pump 11 starts working, and the first solenoid valve 7 and the second solenoid valve 14 connected to the sampling barrel 3 are controlled by the PLC controller to start sampling;

[0068] S4, after the sampling is completed, the PLC controller is used to control the winding mechanism to drive the sampling head 4 to pull back;

[0069] The sampling distance is set to be the same or different for each sampling barrel 3 in turn during the setting. During sampling, when the sampling distance of each sampling barrel 3 is set to be the same, when the distance of the sampling head 4 lowered reaches the set distance, the first solenoid valve 7 and the second solenoid valve 14 on half of the sampling barrels 3 are started first, and the air pump 11 is started to sample half of the sampling barrels 3. After the sampling of half of the sampling barrels 3 is completed, this half of the sampling barrels 3 is used to store the residual water in the sampling tube 9, and then the other half of the sampling barrels 3 are started in the same way, and this part of the sampling barrels 3 is used to collect the sampled samples;

[0070] When the sampling distances of multiple sampling barrels 3 are different, when the sampling head 4 is lowered to a sampling distance set for the first sampling barrel 3, first start the first solenoid valve 7 and the second solenoid valve 14 corresponding to the sampling barrel 3 adjacent to the first sampling barrel 3, and then start the air pump 11 to extract air and sample to transport the residual liquid in the sampling tube 9 to the sampling barrel 3 adjacent to the first sampling barrel 3, and then close the first solenoid valve 7 and the second solenoid valve 14 corresponding to the sampling barrel 3 adjacent to the first sampling barrel 3, and at the same time open the first solenoid valve 7 and the second solenoid valve 14 corresponding to the first sampling barrel 3 to take samples, and close the first solenoid valve 7 and the second solenoid valve 14 corresponding to the first sampling barrel 3 after the sampling is completed; the winding mechanism continues to lower the sampling head 4, and when the sampling head 4 is lowered to the sampling distance set for the second sampling barrel, the second sampling barrel 3 is sampled using the same sampling method as the first sampling barrel 3, and this is repeated until all sampling barrels 3 are sampled.

[0071] It should be noted that the counterweight floating platform 1 is a common suspended material in the prior art, and by adding counterweights, it can be stably suspended above the water surface. No further details will be given here. The counterweight floating platform 1 is placed in the discharge water area and can be located at the detection location through water flow, manual placement or external drive.

[0072] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein; and these modifications or substitutions do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A device for sampling microorganisms of brine from a mineral-containing salt lake, characterized in that: The invention comprises a counterweight floating platform (1), wherein a base (2) is fixed at the lower end of the counterweight floating platform (1), a bottom plate (19) is fixed at the lower end of the base (2), a plurality of sampling barrels (3) are installed at equal distances around the base (2), each sampling barrel (3) is installed with a discharge faucet (5) for discharging liquid, an air pump (11) and a PLC controller are installed inside the counterweight floating platform (1), the plurality of sampling barrels (3) are connected to the air extraction end of the air pump (11) through a first branch pipe (8), and a first solenoid valve (7) is installed on the first branch pipe (8), a winding mechanism is installed on the base (2), a sampling tube (9) is wound on the winding mechanism, and one end of the sampling tube (9) is connected to a sampling head (4) capable of performing preliminary filtration on salt lake brine; The other end of the sampling tube (9) is connected to the lower ends of the plurality of sampling barrels (3) through a connecting mechanism, and the connecting mechanism is provided with second solenoid valves (14) corresponding in number to the plurality of sampling barrels (3) and controlling the entry of samples into the sampling barrels (3). A laser distance measuring mechanism (10) is detachably mounted on the lower surface of the bottom plate (19), and a reflecting plate (20) is mounted on the upper end of the sampling head (4), the reflecting plate (20) being used to reflect the laser signal emitted by the laser distance measuring mechanism (10). The PLC controller is electrically connected to the plurality of first solenoid valves (7), the second solenoid valve (14), the laser distance measuring mechanism (10), the air pump (11) and the winding mechanism, respectively, and the first solenoid valve (7) and the second solenoid valve (14) connected to each of the sampling barrels (3) work synchronously, and one end of the PLC controller is electrically connected to a storage battery.

2. A device for sampling microorganisms of mineral-containing salt lake brine according to claim 1, characterized in that: When setting the sampling distance, the PLC controller sets the same or different sampling distances for each sampling bucket (3).

3. A device for sampling microorganisms of mineral-containing salt lake brine according to claim 2, characterized in that: The sampling barrel (3) comprises a barrel body (301), a piston block (302) matching the inner diameter of the barrel body (301) is slidably arranged inside the barrel body (301), and a label paper (23) is arranged on the outer wall of the sampling barrel (3).

4. A device for sampling microorganisms of mineral-containing salt lake brine according to claim 3, characterized in that: The sampling head (4) comprises an upper cover (402) and a lower cover (401) made of stainless steel. The upper cover (402) and the lower cover (401) are connected by screws after being plugged in. A filter plate (403) is detachably installed between the upper cover (402) and the lower cover (401). Injection holes (405) are provided around the lower cover (401). The reflector (20) is fixed on the upper surface of the upper cover (402). The lower end of the sampling tube (9) is connected to the upper end of the upper cover (402).

5. A device for sampling microorganisms of mineral-containing salt lake brine according to claim 4, characterized in that: A third solenoid valve (24) is installed on the sampling tube (9) located at the upper cover (402), and the third solenoid valve (24) is electrically connected to the PLC controller. The filter plate (403) is configured as a grid plate or a non-woven filter screen plate.

6. A device for sampling microorganisms of mineral-containing salt lake brine according to claim 5, characterized in that: The bottom plate (19) and the base (2) are combined to form a cavity (15), the connecting mechanism is located inside the cavity (15), and the connecting mechanism comprises a second branch pipe (12) connected to the lower end of each sampling barrel (3) and a connecting head (13) connected to the winding mechanism, a plurality of the second branch pipes (12) are distributed at equal angles on the periphery of the connecting head (13), one end of the connecting head (13) is connected to the sampling tube (9) on the winding mechanism, and the second solenoid valve (14) is located on the second branch pipe (12) near the connecting head (13).

7. A device for sampling microorganisms of mineral-containing salt lake brine according to claim 6, characterized in that: The base (2) is provided with a groove connected to the cavity (15); the winding mechanism comprises a motor (17) installed on one side of the groove and a hollow winding roller (16) rotatably installed inside the groove; the other end of the sampling tube (9) is inserted into the winding roller (16) and led out from the end of the winding roller (16) to be connected to the connector (13); a transmission gear (18) is installed on the outer wall of one end of the winding roller (16) and on the output shaft of the motor (17), and the two transmission gears (18) are meshed.

8. The device for sampling microorganisms of mineral-containing salt lake brine according to claim 7, characterized in that: The counterweight floating platform (1) is provided with an installation cavity (22) for installing an air supply pump (11), an exhaust hole (21) is provided at the upper end of the installation cavity (22), and the storage battery and the PLC controller are both located inside the installation groove.

9. A device for sampling microorganisms of mineral-containing salt lake brine according to claim 8, characterized in that: The laser distance measuring mechanism (10) comprises a laser distance measuring sensor (1003), the outer portion of the laser distance measuring sensor (1003) is sleeved with a protective cover (1001), a transparent plate (1002) is provided on one side of the protective cover (1001) located at the emission end of the laser distance measuring sensor (1003), a cover plate (1005) is covered on the side of the protective cover (1001) opposite to the transparent plate (1002), the cover plate (1005) is in contact with the lower surface of the bottom plate (19), and the protective cover (1001) is detachably connected to the bottom plate (19) via bolts; Bolt components are installed on the edges of the side of the protective cover (1001) opposite to the transparent plate (1002), and the bolt components include a stud (1006) and a nut (1007). One end of the stud (1006) passes through the cover plate (1005) and the bottom plate (19) and is threadedly connected to the nut (1007), and a sealing ring (1004) is provided between the cover plate (1005) and the protective cover (1001).

10. A method for sampling microorganisms in brine of a mineral-containing salt lake, based on a device for sampling microorganisms in brine of a mineral-containing salt lake according to any one of claims 1 to 9, characterized in that: The steps include: S1, setting the sampling distance parameters for each sampling bucket (3) through a PLC controller; S2, controlling the winding mechanism to work through the PLC controller, so that the winding mechanism drives the sampling head (4) to be lowered; S3, monitoring the distance between the sampling head (4) and the bottom plate (19) in real time through the laser distance measuring mechanism (10); when the distance between the sampling head (4) and the bottom plate (19) reaches the set sampling distance, the air pump (11) starts working, and controls the first solenoid valve (7) and the second solenoid valve (14) connected to the sampling barrel (3) to start sampling through the PLC controller; S4, after the sampling is completed, the PLC controller is used to control the reeling mechanism to drive the sampling head (4) to pull back; The sampling distance is set to be the same or different for each sampling barrel (3) in sequence during the setting. During sampling, when the sampling distance of each sampling barrel (3) is set to be the same, after the distance the sampling head (4) is lowered reaches the set distance, the first solenoid valve (7) and the second solenoid valve (14) on half of the sampling barrels (3) are first started, and the air pump (11) is started to sample half of the sampling barrels (3). After the sampling of half of the sampling barrels (3) is completed, the half of the sampling barrels (3) are used to store the residual moisture in the sampling tube (9), and then the other half of the sampling barrels (3) are started in the same way, and these sampling barrels (3) are used to collect the sampled samples. When the sampling distances of the plurality of sampling barrels (3) are different, when the distance the sampling head (4) is lowered reaches the sampling distance set for the first sampling barrel (3), the first solenoid valve (7) and the second solenoid valve (14) corresponding to the sampling barrel (3) adjacent to the first sampling barrel (3) are first started, and then the air pump (11) is started to extract air for sampling and transport the residual liquid in the sampling tube (9) to the sampling barrel (3) adjacent to the first sampling barrel (3), and then the first solenoid valve (7) and the second solenoid valve corresponding to the sampling barrel (3) adjacent to the first sampling barrel (3) are closed. (14), and simultaneously open the first solenoid valve (7) and the second solenoid valve (14) corresponding to the first sampling barrel (3) to take samples. After the sampling is completed, close the first solenoid valve (7) and the second solenoid valve (14) corresponding to the first sampling barrel (3); the winding mechanism continues to lower the sampling head (4), and when the sampling head (4) is lowered to the sampling distance set for the second sampling barrel, the second sampling barrel (3) is sampled using the same sampling method as the first sampling barrel (3), and this is repeated until all sampling barrels (3) are sampled.

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