Culture system for low-pressure carbon dioxide and use method of culture system

Abstract

The culture system comprises an incubator and a sealing cover, the sealing cover is clamped at the opening position of the top end of the incubator through a sealing groove, a control assembly is arranged on the sealing cover, and the sealing cover is communicated with a first vent valve and a second vent valve; the control assembly simultaneously controls the first vent valve and the second vent valve, the first vent valve is connected to the vacuum pump through a pipeline, the second vent valve is connected to the carbon dioxide source through a pipeline, and the incubator and the sealing cover are connected through the locking assembly; when the device is used for anaerobic bacteria culture, a culture medium and a carbon dioxide gas production bag are firstly put into the culture box, then the pressure gauge is calibrated, and the control assembly can automatically adjust the first vent valve and the second vent valve in real time according to the scale change of the pressure gauge, so that the operation steps of experimenters are saved, and meanwhile, the adjusting precision of the device is improved.

Description

Technical Field

[0001] The present invention relates to the field of low-pressure culture technology, and more specifically, to a culture system for low-pressure carbon dioxide and a method for using the system. Background Art

[0002] Anaerobic culture refers to the culture of microorganisms in a state of isolation from molecular oxygen. It is applicable to facultative anaerobes and obligate anaerobes. Existing incubators for culturing anaerobic bacteria mostly use vacuum pumps to directly extract air to discharge oxygen from the container.

[0003] However, after the existing culture method is completed, a small amount of oxygen usually remains in the container. Since the culture objects are anaerobic bacteria, the oxygen in the incubator will continue to affect the reproduction of the culture objects during the entire culture process. For objects that require long-term culture, it will greatly reduce their culture efficiency and thus reduce the use effect of the culture system. Summary of the invention

[0004] The present invention provides a culture system for low-pressure carbon dioxide and a method for using the same, in order to solve the problem in the prior art that after the gas is exhausted, a small amount of oxygen usually remains in the container, and the oxygen in the incubator will continue to affect the reproduction of the cultured objects, thereby reducing the use effect of the culture system.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A culture system for low-pressure carbon dioxide comprises a culture box and a sealing cover, wherein the sealing cover is clamped at the top opening position of the culture box through a sealing groove, a control component is arranged on the sealing cover, a first ventilation valve and a second ventilation valve are connected to the sealing cover, the control component controls the first ventilation valve and the second ventilation valve at the same time, the first ventilation valve is connected to a vacuum pump through a pipeline, the second ventilation valve is connected to a carbon dioxide source through a pipeline, and the culture box and the sealing cover are connected through a locking component.

[0007] Preferably, the control component includes a first light measuring plate and a second light measuring plate arranged at the top of the sealing cover, the first light measuring plate is close to the first ventilation valve, the second light measuring plate is close to the second ventilation valve, a two-way telescopic rod is arranged at the top of the sealing cover, two connecting frames are connected to the output end of the two-way telescopic rod, wrenches are arranged on the outside of the first ventilation valve and the second ventilation valve, and the two connecting frames are respectively clamped on the outside of the two wrenches.

[0008] Preferably, the control component is controlled by a guide component, and the guide component includes a pressure gauge arranged at the top of the sealing cover, a guide rail is arranged on the outside of the pressure gauge, a rotating frame is arranged on the top of the guide rail, a pair of clips are arranged at the bottom of the rotating frame, two of the clips are clamped on the outside of the guide rail, a magnetic block is arranged at one end of the rotating frame, and the magnetic block moves synchronously with the pointer inside the pressure gauge.

[0009] Preferably, a moving rod is passed through the rotating frame, a guide button is provided at one end of the moving rod, a light source is provided on the outside of the guide button, a guide sleeve is provided on the outside of the pressure gauge, the guide button is clamped on the side wall of the guide sleeve, and a spiral groove, two arc grooves and a top groove are provided on the guide sleeve from low to high, and the two ends of the spiral groove and the arc groove correspond to the negative one scale and the scale under atmospheric pressure of the pressure gauge in the angular direction, respectively, and the spiral groove, arc groove and top groove are arranged in an S shape and are connected.

[0010] Preferably, positioning grooves are provided at both ends of the spiral groove, the two arc grooves and the top groove, a rotatably connected reversing plate is provided on one side of the positioning groove, the top groove corresponds to the 0.5 scale and the negative one scale of the pressure gauge in the angular direction, a reset groove is connected to the end of the top groove corresponding to the 0.5 scale of the pressure gauge, a longitudinal groove is provided on the side wall of the guide sleeve, and the longitudinal groove is connected to the bottom end of the spiral groove.

[0011] Preferably, the locking assembly includes a locking block arranged on the outside of the sealing cover, the outer side of the locking block is rotatably connected to a rotating plate, a plurality of fixed blocks are arranged on the top of the incubator, each of the fixed blocks is rotatably connected to a limit card, the top of each limit card is connected to a pressure plate, the limit card is arranged on the top of the locking block, an elastic ring is arranged on the top surface of the fixed block, and the top surface of the elastic ring is in contact with the bottom surface of the sealing cover.

[0012] Preferably, a method for using a culture system for low-pressure carbon dioxide comprises the following steps:

[0013] S1: Method: Place the culture medium, carbon dioxide gas generating bag, and sterile water into the incubator, open the second ventilation valve, and start the vacuum pump to extract the air;

[0014] S2: When the pressure gauge reading is minus one, close the second ventilation valve and open the first ventilation valve, enable the carbon dioxide source, and inject carbon dioxide;

[0015] S3: When the pressure displayed is atmospheric pressure, close the first ventilation valve and open the second ventilation valve, and start the vacuum pump to evacuate air again;

[0016] S4: When the pressure indicator shows negative one, close the second vent valve and open the first vent valve, and inject carbon dioxide again. When the pressure gauge shows zero point five, close the first vent valve and the second vent valve. At this time, the carbon dioxide gas generating bag will gradually absorb the remaining oxygen and release carbon dioxide. At this time, there is only carbon dioxide and a very small amount of rare gas in the vacuum tank, so that the inside of the incubator achieves excellent anaerobic conditions, providing good culture conditions for the culture of anaerobic bacteria.

[0017] The principle and beneficial effects of this technical solution:

[0018] (1) The control component provided in the present invention can automatically adjust the first vent valve and the second vent valve in real time through the scale change of the pressure gauge, so as to save the operating steps of the experimenter and improve the adjustment accuracy of the device. At the same time, the incubator used in the present invention is a vacuum tank, which has relatively low manufacturing and maintenance costs and is easy to operate. It has a large capacity and can process more products, thereby improving the culture efficiency.

[0019] (2) The locking assembly provided in the present invention can provide a sealed connection between the sealing cover and the incubator, thereby improving the airtightness of the device when in use. After the sealing cover is placed on the top of the incubator, the limit card is rotated along the fixed block. During the rotation, the sealing cover is pressed down to slightly compress the elastic ring, and then the limit card is locked to the top of the locking block. At this time, the rotating plate is rotated along the outer side of the locking block, and the position of the limit card is locked to complete the sealed connection between the sealing cover and the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 It is a schematic diagram of the structure after the present invention is split;

[0022] Figure 3 for Figure 2 Schematic diagram of the enlarged structure of the A area in the middle;

[0023] Figure 4 for Figure 2 Schematic diagram of the enlarged structure of the middle B area;

[0024] Figure 5 for Figure 2 Schematic diagram of the enlarged structure of the middle C region;

[0025] Figure 6 It is a structural schematic diagram of the guide sleeve in the present invention;

[0026] The figure marks in the drawings of the specification include: 1. sealing cover; 2. incubator; 3. first ventilation valve; 4. longitudinal groove; 5. positioning groove; 6. reversing plate; 7. magnetic block; 8. moving rod; 9. first light measuring plate; 10. second ventilation valve; 11. two-way telescopic rod; 12. connecting frame; 13. rotating frame; 14. guide button; 15. clamp; 16. second light measuring plate; 17. guide rail; 18. wrench; 19. pressure plate; 20. limit card; 21. sealing groove; 22. elastic ring; 23. fixing block; 24. reset groove; 25. top groove; 26. arc groove; 27. spiral groove; 28. guide sleeve; 29. ​​rotating plate; 30. locking block; 31. pressure gauge. DETAILED DESCRIPTION

[0027] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments:

[0028] Example:

[0029] like Figures 1 to 6 As shown, the present invention provides a culture system for low-pressure carbon dioxide and a method of using the same, comprising a culture box 2 and a sealing cover 1, wherein the sealing cover 1 is clamped at the top opening of the culture box 2 through a sealing groove 21, a control component is arranged on the sealing cover 1, a first ventilation valve 3 and a second ventilation valve 10 are connected to the sealing cover 1, the control component controls the first ventilation valve 3 and the second ventilation valve 10 simultaneously, the first ventilation valve 3 is connected to a carbon dioxide source through a pipeline, the second ventilation valve 10 is connected to a vacuum pump through a pipeline, and the culture box 2 and the sealing cover 1 are connected through a locking component.

[0030] like Figure 1 , Figure 2 and Figure 4 As shown, the control component includes a first light measuring plate 9 and a second light measuring plate 16 arranged on the top of the sealing cover 1, the first light measuring plate 9 is close to the first ventilation valve 3, the second light measuring plate 16 is close to the second ventilation valve 10, a two-way telescopic rod 11 is arranged on the top of the sealing cover 1, and two connecting frames 12 are connected to the output ends of the two-way telescopic rod 11, and wrenches 18 are arranged on the outsides of the first ventilation valve 3 and the second ventilation valve 10, and the two connecting frames 12 are respectively clamped on the outsides of the two wrenches 18.

[0031] like Figure 1 and Figure 4 As shown, the control component is controlled by the guide component, and the guide component includes a pressure gauge 31 arranged at the top of the sealing cover 1, a guide rail 17 is arranged on the outside of the pressure gauge 31, a rotating frame 13 is arranged on the top of the guide rail 17, a pair of clamps 15 are arranged at the bottom of the rotating frame 13, and the two clamps 15 are clamped on the outside of the guide rail 17. A magnetic block 7 is arranged at one end of the rotating frame 13, and the magnetic block 7 moves synchronously with the internal pointer of the pressure gauge 31.

[0032] like Figure 4 and Figure 6As shown, a moving rod 8 is inserted into the rotating frame 13, a guide button 14 is provided at one end of the moving rod 8, a light source is provided on the outside of the guide button 14, a guide sleeve 28 is provided on the outside of the pressure gauge 31, the guide button 14 is clamped on the side wall of the guide sleeve 28, and a spiral groove 27, two arc grooves 26 and a top groove 25 are provided on the guide sleeve 28 from low to high, and the two ends of the spiral groove 27 and the arc groove 26 correspond to the negative one scale and the scale under the atmospheric pressure state of the pressure gauge 31 in the angular direction, respectively, and the spiral groove 27, the arc groove 26 and the top groove 25 are arranged in an S shape and are connected.

[0033] like Figure 3 and Figure 6 As shown, the spiral groove 27, the two arc grooves 26 and the top groove 25 are all provided with positioning grooves 5 at both ends, and a rotatably connected reversing plate 6 is provided on one side of the positioning groove 5. The top groove 25 corresponds to the 0.5 scale and the negative one scale of the pressure gauge 31 in the angular direction, and the end of the top groove 25 corresponding to the 0.5 scale of the pressure gauge 31 is connected to the reset groove 24. A longitudinal groove 4 is provided on the side wall of the guide sleeve 28, and the longitudinal groove 4 is connected to the bottom end of the spiral groove 27.

[0034] like Figure 1 , Figure 2 and Figure 5 As shown, the locking assembly includes a locking block 30 arranged on the outer side of the sealing cover 1, and the outer side of the locking block 30 is rotatably connected to a rotating plate 29, and a plurality of fixed blocks 23 are arranged on the top of the incubator 2, and each fixed block 23 is rotatably connected to a limit card 20, and each limit card 20 is connected to a pressure plate 19 on the top, and the limit card 20 is clamped on the top of the locking block 30, and an elastic ring 22 is arranged on the top surface of the fixed block 23, and the top surface of the elastic ring 22 contacts the bottom surface of the sealing cover 1.

[0035] The locking assembly can provide a sealed connection between the sealing cover 1 and the incubator 2, thereby improving the airtightness of the device when in use. After the sealing cover 1 is placed on the top of the incubator 2, the limit card 20 is rotated along the fixing block 23. During the rotation, the sealing cover 1 is pressed downward to slightly compress the elastic ring 22, and then the limit card 20 is clamped to the top of the locking block 30. At this time, the rotating plate 29 is rotated along the outer side of the locking block 30, and the position of the limit card 20 is locked to complete the sealed connection between the sealing cover 1 and the incubator 2.

[0036] A method for using a low-pressure carbon dioxide culture system, comprising the following steps:

[0037] S1: Method: Place the culture medium, the carbon dioxide gas generating bag, and sterile water into the incubator, open the second ventilation valve 10, and start the vacuum pump to extract the air;

[0038] S2: When the pressure gauge reading is minus one, close the second ventilation valve 10 and open the first ventilation valve 3, enable the carbon dioxide source, and inject carbon dioxide;

[0039] S3: When the pressure displayed is atmospheric pressure, close the first vent valve 3 and open the second vent valve 10, and start the vacuum pump to evacuate air again;

[0040] S4: When the pressure indicator shows negative one, close the second ventilation valve 10 and open the first ventilation valve 3, and inject carbon dioxide again. When the pressure gauge shows zero point five, close the first ventilation valve 3. At this time, the carbon dioxide gas generating bag will gradually absorb the remaining oxygen and release carbon dioxide. At this time, there is only carbon dioxide and a very small amount of rare gas in the vacuum tank, so that the inside of the incubator reaches excellent anaerobic conditions, providing good culture conditions for the culture of anaerobic bacteria.

[0041]

[0042] Comparison table of the effects of culturing target bacteria using the present invention and traditional methods

[0043] The specific usage and function of this embodiment are as follows:

[0044] The control component provided in the present invention can automatically adjust the first ventilation valve 3 and the second ventilation valve 10 in real time through the scale change of the pressure gauge, so as to save the operating steps of the experimenter and improve the adjustment accuracy of the device. When using the device for anaerobic bacteria culture, first put the culture medium, carbon dioxide gas production bag, and sterile water into the incubator 2, and then calibrate the pressure gauge. After the calibration is completed, since a rotating frame 13 is provided at the top of the guide rail 17 outside the pressure gauge, and the magnetic block 7 provided at one end of the rotating frame 13 rotates synchronously with the pointer inside the pressure gauge, the rotating frame 13 will drive the guide button 14 provided at one end thereof to move synchronously, so that the guide button 14 moves to the positioning groove 5 opened at the bottom end of the spiral groove 27, and the guide button 14 moves to the top of the positioning groove 5 at the bottom end of the spiral groove 27 and When falling in the vertical direction, the second photometric plate 16 will monitor the vertical displacement of the light source outside the guide button 14. At this time, the two-way telescopic rod 11 in the control component will drive the output rod close to the second ventilation valve 10 to extend and retract. Since the connecting frame 12 connected to the output end of the two-way telescopic rod 11 is clamped on the wrench 18 provided on the outside of the second ventilation valve 10, the extension and retraction of the output rod will drive the wrench 18 to rotate, so that the second ventilation valve 10 is opened. At this time, the vacuum pump will start to extract the air in the incubator 2, and the pressure gauge will also start to deflect. Similarly, it can be known that the guide button 14 will move along the spiral groove 27. When the guide button 14 passes the bottom end of the reversing plate 6, it will lift the reversing plate 6 until it slides into another positioning groove 5 opened at the top of the spiral groove 27.

[0045] When the guide button 14 is inserted into the positioning groove 5 provided at the top of the spiral groove 27, similarly, the guide button 14 will also slightly deviate in the vertical direction. Since the two ends of the spiral groove 27 correspond to the minus one scale of the pressure gauge and the scale under the atmospheric pressure state in the angular direction, it also indicates that the internal pressure of the incubator 2 has dropped to the corresponding value of the minus one scale of the pressure gauge. At this time, the second photometric plate 16 will control the first ventilation valve 3 to open and close the second ventilation valve 10, so that the carbon dioxide source fills carbon dioxide into the incubator 2, so that the guide button 14 moves along the arc groove 26, and at the same time, the position of the guide button 14 in the vertical direction is changed with the help of the reversing plate 6. Similarly, when the guide button 14 moves to the positioning groove 5 at one end of the arc groove 26 at a lower position, the above-mentioned steps of exhausting and filling with carbon dioxide are repeated. Since the density of oxygen is lower than that of carbon dioxide, the residual oxygen in the incubator 2 can be greatly reduced after repeating this process.

[0046] After the gas in the incubator 2 is emptied for the second time, the guide button 14 is located in the positioning groove 5 at one end of the top groove 25. The guide button 14 will slide along the top groove 25 again under the drive of the barometer until it slides to the position at the other end of the top groove 25 corresponding to the 0.5 scale of the pressure gauge. After being inserted into the positioning groove 5 at this position, the first photometric plate 9 will detect the movement of the guide button 14 and change the first ventilation valve 3 and the second ventilation valve 10 to a closed state. At this time, the environmental adjustment in the incubator 2 is completed, and the final residual small amount of oxygen in the incubator 2 will also be converted into carbon dioxide under the action of the carbon dioxide gas producing bag, and finally the environment in the incubator 2 will be converted into a low-pressure anaerobic environment, thereby greatly improving the culture efficiency of the target bacteria.

[0047] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A culture system for low-pressure carbon dioxide, characterized in that: The invention comprises an incubator (2) and a sealing cover (1), wherein the sealing cover (1) is clamped at the top opening position of the incubator (2) through a sealing groove (21), a control component is arranged on the sealing cover (1), a first ventilation valve (3) and a second ventilation valve (10) are connected to the sealing cover (1), the control component controls the first ventilation valve (3) and the second ventilation valve (10) simultaneously, the first ventilation valve (3) is connected to a carbon dioxide source through a pipeline, the second ventilation valve (10) is connected to a vacuum pump through a pipeline, and the incubator (2) and the sealing cover (1) are connected through a locking component.

2. A culture system for low-pressure carbon dioxide according to claim 1, characterized in that: The control component comprises a first light measuring plate (9) and a second light measuring plate (16) arranged at the top of the sealing cover (1), wherein the first light measuring plate (9) is close to the first ventilation valve (3), and the second light measuring plate (16) is close to the second ventilation valve (10). A bidirectional telescopic rod (11) is arranged at the top of the sealing cover (1), and two connecting frames (12) are connected to the output end of the bidirectional telescopic rod (11). Wrenches (18) are arranged on the outside of the first ventilation valve (3) and the second ventilation valve (10), and the two connecting frames (12) are respectively clamped on the outside of the two wrenches (18).

3. A culture system for low-pressure carbon dioxide according to claim 2, characterized in that: The control component is controlled by a guide component, the guide component comprising a pressure gauge (31) arranged at the top end of the sealing cover (1), a guide rail (17) being arranged on the outside of the pressure gauge (31), a rotating frame (13) being arranged at the top end of the guide rail (17), a pair of clamps (15) being arranged at the bottom end of the rotating frame (13), two of the clamps (15) being clamped on the outside of the guide rail (17), a magnetic block (7) being arranged at one end of the rotating frame (13), and the magnetic block (7) moving synchronously with a pointer inside the pressure gauge (31).

4. A cultivation system for low-pressure carbon dioxide according to claim 3, characterized in that: A moving rod (8) is inserted into the rotating frame (13), a guide button (14) is provided at one end of the moving rod (8), a light source is provided on the outer side of the guide button (14), a guide sleeve (28) is provided on the outer side of the pressure gauge (31), the guide button (14) is clamped on the side wall of the guide sleeve (28), and the guide sleeve (28) is provided with a spiral groove (27), two arc grooves (26) and a top groove (25) in order from low to high, the two ends of the spiral groove (27) and the arc groove (26) respectively correspond to the minus one scale and the scale under the atmospheric pressure state of the pressure gauge (31) in the angular direction, and the spiral groove (27), the arc groove (26) and the top groove (25) are arranged in an S shape and connected.

5. A culture system for low-pressure carbon dioxide according to claim 4, characterized in that: The spiral groove (27), the two arc grooves (26) and the top groove (25) are provided with positioning grooves (5) at both ends. A rotatably connected reversing plate (6) is provided on one side of the positioning groove (5). The top groove (25) corresponds to the zero point five scale and the negative one scale of the pressure gauge (31) in the angular direction. The top groove (25) is connected to a reset groove (24) at one end corresponding to the zero point five scale of the pressure gauge (31). A longitudinal groove (4) is provided on the side wall of the guide sleeve (28). The longitudinal groove (4) is connected to the bottom end of the spiral groove (27).

6. A culture system for low-pressure carbon dioxide according to claim 5, characterized in that: The locking assembly comprises a locking block (30) arranged on the outside of the sealing cover (1), the locking block (30) being rotatably connected to a rotating plate (29) on the outside, a plurality of fixed blocks (23) being arranged on the top of the incubator (2), each of the fixed blocks (23) being rotatably connected to a limiting card (20), the top of each limiting card (20) being connected to a pressing plate (19), the limiting card (20) being arranged on the top of the locking block (30), the top surface of the fixed block (23) being arranged to have an elastic ring (22), the top surface of the elastic ring (22) being in contact with the bottom surface of the sealing cover (1).

7. A method for using a low-pressure carbon dioxide culture system as claimed in claim 6, characterized in that: The following steps are involved: S1: Method: Place the culture medium, the carbon dioxide gas generating bag, and sterile water into the incubator, open the second ventilation valve (10), and start the vacuum pump to extract the air; S2: When the pressure gauge reading is minus one, close the second ventilation valve (10) and open the first ventilation valve (3), activate the carbon dioxide source, and inject carbon dioxide; S3: When the pressure displayed is atmospheric pressure, close the first ventilation valve (3) and open the second ventilation valve (10), and start the vacuum pump to evacuate air again; S4: When the pressure indicator shows negative one, close the second ventilation valve (10) and open the first ventilation valve (3), and inject carbon dioxide again. When the pressure gauge shows zero point five, close the first ventilation valve (3). At this time, the carbon dioxide gas generating bag will gradually absorb the remaining oxygen and release carbon dioxide. At this time, there is only carbon dioxide and a very small amount of rare gas in the vacuum tank, so that the inside of the incubator reaches an excellent anaerobic condition, providing good culture conditions for the culture of anaerobic bacteria.

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