Yersinia culture device with uniform temperature control function

By using a servo motor-driven placement tray and heating rod system, combined with a gas exchange system, the problem of uneven temperature in existing devices has been solved, achieving uniform temperature control and stable gas temperature for Yersinia bacteria culture, thus improving the culture effect.

CN119265031AInactive Publication Date: 2025-01-07NANJING DRUM TOWER HOSPITAL
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Patent Information

Application Number
CN202411815312.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-11
Publication Date
2025-01-07
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

Existing constant temperature bacterial culture devices are prone to uneven temperature during the culture process due to damage or insensitivity of temperature control elements, which affects the culture effect of Yersinia.

Method used

A Yersinia bacteria culture device with uniform temperature control function was designed. The placement plate is driven by a servo motor to perform linear reciprocating motion. Combined with a ring array of heating rods and a gear system, a temperature-controlled cylindrical area is formed. A gas exchange system ensures that the culture dish is in a uniform temperature environment.

Benefits of technology

This method achieves uniform temperature within the petri dish and stable gas temperature, improving the cultivation effect of Yersinia and avoiding uneven temperature distribution.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to the technical field of bacterial culture, in particular to a yersinia culture device with a uniform temperature control function. Comprising a base, the base is fixedly connected with a culture shell, a servo motor is fixedly connected in the culture shell, an output shaft of the servo motor is fixedly connected with a first gear, a mounting column is slidably connected in the culture shell through a connecting piece, and the mounting column is detachably connected with a placing disc. A second gear meshed with the adjacent first gear is rotationally connected into the culture shell through a connecting piece, the second gear is fixedly connected with a clamping shaft, a sliding groove is formed in the bottom of the mounting column, and the clamping shaft is in sliding connection with the adjacent sliding groove. The placing disc drives the culture dishes on the placing disc to do up-and-down linear reciprocating motion, the positions of the culture dishes are continuously changed, the culture dishes are subjected to heat exchange with hot air at multiple positions, and the temperature uniformity degree of the culture dishes in the culture period is improved.
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Description

Technical Field

[0001] The invention relates to the technical field of bacterial culture, in particular to a Yersinia culture device with a uniform temperature control function. Background Art

[0002] Yersinia is a human pathogen widely present in the environment and animal hosts. It is a type of Yersinia. It is a zoonosis that can be transmitted through the fecal-oral route. It usually causes mild self-limited enteritis and terminal ileitis, and occasionally causes chronic inflammatory diseases such as arthritis and erythema nodosum. The optimal growth temperature is 22-25°C, but it can also grow at the core body temperature of mammals at 37°C. In order to study the behavior and response of Yersinia at different temperatures, researchers need to culture Yersinia at two temperatures at the same time to observe the status of Yersinia. When culturing in existing constant temperature bacterial culture devices, staff place the culture dishes directly on the racks, which are always fixed during the culture process. When some of the temperature control components inside are damaged or insensitive, this method can easily lead to uneven temperatures in all culture dishes, thereby affecting the cultivation of bacteria. Summary of the invention

[0003] In order to overcome the shortcomings mentioned in the above background technology, the present invention provides a Yersinia culture device with a uniform temperature control function.

[0004] The technical solution of the present invention is: a Yersinia culture device with a uniform temperature control function, comprising a base, the base is fixedly connected to a control terminal, the base is fixedly connected to a mirror-distributed culture shell, the mirror-distributed culture shells are all hinged with a cover, the mirror-distributed culture shells are all fixedly connected with a servo motor, the mirror-distributed servo motors are all electrically connected to the control terminal, the output shaft of the servo motor is fixedly connected with a first gear, the culture shell is slidably connected with a mounting column through a connecting piece, the mounting column is detachably connected with a placement plate of a linear array, the culture shell is rotatably connected with a second gear meshing with an adjacent first gear through a connecting piece, the second gear is fixedly connected with a clamping shaft, a slide groove is provided at the bottom of the mounting column, the clamping shaft is slidably connected to the adjacent slide groove, and the mirror-distributed culture shells are all provided with a first temperature control component for maintaining a uniform internal temperature.

[0005] Furthermore, the radius of the mounting column decreases gradually from the bottom to the top, and the inner circle radius of the placement plate decreases gradually from the bottom to the top, so as to keep the distance between the placement plates of the linear array consistent.

[0006] Furthermore, the first temperature control component includes a first gear ring, which is rotatably connected to the interior of the adjacent culture shell, and the first gear ring is fixedly connected to a fixing frame of an annular array, and the fixing frames of the annular array are all fixedly connected to a first heating rod electrically connected to the control terminal, and the first gear ring is meshed with the first gear, and a second temperature control component is commonly arranged on the first heating rods of the annular array.

[0007] Furthermore, the second temperature control component includes second heating rods in a circular array, the second heating rods in the circular array are electrically connected to the control terminal, the second heating rods in the circular array are respectively rotatably connected to adjacent first heating rods, the second heating rods in the circular array are fixedly connected to a third gear, a second gear ring is rotatably connected inside the culture shell, the third gears in the circular array are meshed with adjacent second gear rings, and the third gears in the circular array are provided with a locking component for locking themselves.

[0008] Furthermore, the locking assembly includes a limiting shaft in an annular array, and the limiting shaft in the annular array is slidably connected to the adjacent third gear through connecting pieces, the second gear ring is provided with through holes in an annular array, and the fixing frame of the annular array is provided with through holes, the limiting shaft is limitedly matched with the through holes on the adjacent second gear ring, and the limiting shaft is limitedly matched with the through holes on the adjacent fixing frame.

[0009] Furthermore, it also includes a pushing component that is distributed in a mirror image and is used to push the gas inside adjacent culture shells respectively. The pushing component includes a plurality of groups of impact cylinders in an annular array, and the plurality of groups of impact cylinders in the annular array are fixedly connected to the inside of adjacent culture shells through connecting pieces. Each group includes a linear array of impact cylinders, and the linear array of impact cylinders are sealed and slidably connected with piston plates. First elastic elements are provided between the linear array of impact cylinders and adjacent piston plates respectively. The impact cylinders and adjacent piston plates cooperate to form a first closed cavity. A pipeline is fixedly connected between two adjacent impact cylinders, and two adjacent first closed cavities are connected through a pipeline. A driving component for driving the movement of the adjacent linear array of piston plates is provided in the culture shell.

[0010] Furthermore, the driving assembly includes a circular array of docking cylinders, which are fixedly connected to the interior of the culture shell through connecting pieces, and the docking cylinders of the circular array are fixedly connected to adjacent impact cylinders through pipes. A piston shaft is sealingly and slidingly connected inside the docking cylinder, and the docking cylinder cooperates with the adjacent piston shaft to form a second closed cavity, which is connected to the adjacent first closed cavity through a pipe. A second elastic element is arranged between the docking cylinder and the adjacent piston shaft, and an extrusion block of an annular array is fixedly connected to the outside of the first gear ring, and the extrusion blocks of the annular array are extruded and cooperated with the piston shafts of the annular array, and a reversing assembly for changing the pushing direction of adjacent impact cylinders is provided on each group of the impact cylinders.

[0011] Furthermore, the reversing assembly includes a linear array of flexible rings, the flexible rings of the linear array are respectively fixedly connected to adjacent impact cylinders, the flexible rings are fixedly connected to reversing cylinders, the reversing cylinders of the linear array are commonly fixedly connected to an adjusting frame, the adjusting frame is slidably connected to a sliding rod, the sliding rod is fixedly connected to the adjacent piston shaft, and a swinging assembly for swinging the adjusting frame is provided on the piston shaft.

[0012] Furthermore, the swing assembly includes a first clamping block, which is slidably connected to the adjacent sliding rod, and a third elastic element is arranged between the first clamping block and the adjacent sliding rod. The adjustment frame is fixedly connected to a second clamping block of a linear array, and a gap is left between two adjacent second clamping blocks. The second clamping blocks of the linear array are squeezed and matched with the adjacent first clamping blocks.

[0013] Furthermore, the spring coefficient of the third elastic element is greater than the sum of the spring coefficients of the flexible rings in adjacent linear arrays.

[0014] Compared with the prior art, the present invention has the following advantages: 1. The present invention drives the culture dish on it to perform linear reciprocating motion up and down by placing the plate, continuously changing the position of the culture dish, so that it can exchange heat with hot air at multiple positions, thereby improving the uniformity of temperature during culture of the culture dish.

[0015] 2. The three first heating rods in the annular array and the three second heating rods in the annular array are rotated to form a temperature-controlled column area, and the adjacent culture dishes are surrounded to the inside, so that the Yersinia in the culture shell is in a uniform temperature environment.

[0016] 3. The three second heating rods are twisted by the three third gears and the second gear ring of the annular array so that the second heating rods are deflected to the inner wall of the culture shell, so as to facilitate the staff to place or take the culture dish.

[0017] 4. The piston plate impacts the gas near the first heating rod, so that the gas at the appropriate temperature promptly replaces the gas at the culture dish, ensuring that the gas at the culture dish is always at the set temperature value, thereby improving the culture effect of Yersinia.

[0018] 5. By making the three reversing cylinders of the linear array perform linear reciprocating motion, the impact direction of the piston plate is changed, the impact range of the gas is increased, and the stability of the gas temperature around the culture dish is further improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention; Figure 2 It is a schematic cross-sectional view of the three-dimensional structure of the culture shell of the present invention; Figure 3 It is a schematic cross-sectional view of the three-dimensional structure of the mounting column of the present invention; Figure 4 It is a schematic diagram of the three-dimensional structure of the slideway of the present invention; Figure 5 It is a schematic diagram of the three-dimensional structure of the first gear ring and the fixing frame of the present invention; Figure 6 It is a schematic diagram of the three-dimensional structure of the second heating rod and the third gear of the present invention; Figure 7 It is a schematic diagram of the three-dimensional structure of the second gear ring and the limiting shaft of the present invention; Figure 8 It is a three-dimensional structural schematic diagram of the fixing frame and the first heating rod of the present invention; Fig. 9 It is a schematic cross-sectional view of the three-dimensional structure of the impact cylinder of the present invention; Fig.10 It is a schematic cross-sectional view of the three-dimensional structure of the sliding rod of the present invention.

[0020] The names and serial numbers of the parts in the figure are: 1-base, 2-control terminal, 3-culture shell, 4-cover, 5-servo motor, 6-first gear, 7-mounting column, 8-placing plate, 9-second gear, 10-clamp shaft, 11-slide, 201-first gear ring, 202-fixed frame, 203-first heating rod, 301-second heating rod, 302-third gear, 303-second gear ring, 304-limiting shaft, 401-impact cylinder, 402-piston plate, 403-first elastic element, 404-docking cylinder, 405-piston shaft, 406-second elastic element, 407-extrusion block, 501-flexible ring, 502-reversing cylinder, 503-adjusting frame, 504-sliding rod, 505-first clamping block, 506-third elastic element, 507-second clamping block. DETAILED DESCRIPTION

[0021] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0022] When the existing constant temperature bacterial culture device is used for culture, the staff directly places the culture dish on the rack, which is always in a fixed state during the culture process. When some of the temperature control components inside are damaged or insensitive, this method can easily lead to uneven temperature in all culture dishes, thereby affecting the culture of bacteria.

[0023] Example 1: A Yersinia culture device with uniform temperature control function, such as Figure 1-Figure 4 As shown, it includes a base 1, a control terminal 2 is fixedly connected to the right side of the base 1, two culture shells 3 distributed in upper and lower mirror images are fixedly connected to the base 1, and the front sides of the two culture shells 3 distributed in upper and lower mirror images are hinged with a cover 4, the bottoms of the two culture shells 3 distributed in upper and lower mirror images are fixedly connected to a servo motor 5, the two servo motors 5 distributed in upper and lower mirror images are electrically connected to the control terminal 2, the output shaft of the servo motor 5 is fixedly connected to a first gear 6, and the two culture shells 3 distributed in upper and lower mirror images are slidably connected with a mounting column 7 through a connecting piece, the mounting column 7 is detachably connected to a placement plate 8 in a linear array, the placement plate 8 is a breathable grid plate, and the placement plate 8 is provided with a ring array of clamping rings (not shown in the figure), and the clamping ring is used to fix the culture dish on the placement plate 8, the bottom radius of the mounting column 7 gradually decreases to the top radius, the mounting column 7 is a truncated cone, and the inner circle radius of the placement plate 8 gradually decreases from the bottom to the top, and the placement plate 8 is sequentially inserted into the mounting column 7 so that the placement plate The mounting column 7 supports and fixes the placement plate 8 of the linear array, simplifies the fixing process, and is convenient for staff to operate. The bottom of the culture shell 3 is rotatably connected to a second gear 9 meshing with the adjacent first gear 6 through a connecting piece, and the upper part of the second gear 9 is fixedly connected to a clamping shaft 10, so that the first gear 6 drives the clamping shaft 10 thereon to rotate through the second gear 9. A slide groove 11 is provided at the bottom of the mounting column 7. The slide groove 11 is spliced ​​by two 180° spiral grooves distributed in a mirror image. The clamping shaft 10 is slidably connected with the adjacent slide groove 11, which is used to make the clamping shaft 10 rotate one circle to drive the mounting column 7 to perform an up and down linear reciprocating motion. The two culture shells 3 distributed in a mirror image are both provided with a first temperature control component for maintaining a uniform internal temperature. The present invention drives the culture dish thereon to perform an up and down linear reciprocating motion through the placement plate 8, continuously changes the position of the culture dish, enables it to exchange heat with hot air at multiple positions, and improves the uniformity of the temperature of the culture dish during cultivation.

[0024] like Figure 5 and Figure 6As shown, the first temperature control component includes a first gear ring 201, which is rotatably connected to the bottom of an adjacent culture shell 3. The first gear ring 201 is fixedly connected to three fixing frames 202 of an annular array. The tops of the three fixing frames 202 of the annular array are fixedly connected to first heating rods 203 electrically connected to the control terminal 2. The first heating rod 203 is L-shaped and is used to surround the bottom and side of the culture dish. The first gear ring 201 is meshed with the first gear 6. The rotation of the first gear 6 drives the first gear ring 201 to rotate, so that the three fixing frames 202 of the annular array drive the first heating rods 203 thereon to rotate synchronously. The three first heating rods 203 of the annular array are commonly provided with a second temperature control component.

[0025] like Figure 5-Figure 8 As shown, the second temperature control component includes three second heating rods 301 in an annular array, and the three second heating rods 301 in the annular array are electrically connected to the control terminal 2. The three second heating rods 301 in the annular array are respectively rotatably connected to the adjacent first heating rods 203, and are used to surround the tops of several culture dishes. The tops of the three second heating rods 301 in the annular array are fixedly connected to the third gear 302, and the top of the culture shell 3 is rotatably connected to the second gear ring 303. The three third gears 302 in the annular array are meshed with the adjacent second gear ring 303, so that the second gear ring 303 drives the corresponding The three third gears 302 of the adjacent annular array rotate, thereby driving the adjacent second heating rod 301 to rotate, so that the second heating rod 301 is deflected from the inner wall of the adjacent culture shell 3 to the middle thereof, which is convenient for the staff to place or take the culture dish. The three third gears 302 of the annular array are commonly provided with a locking assembly for locking themselves. The three first heating rods 203 of the annular array and the three second heating rods 301 of the annular array rotate to form a temperature-controlled columnar area, and several adjacent culture dishes are surrounded to the inside, so that the Yersinia in the culture shell 3 is in a uniform temperature environment.

[0026] like Figure 5-Figure 8 As shown, the locking assembly includes three limiting shafts 304 in an annular array, and the three limiting shafts 304 in the annular array are respectively slidably connected to the adjacent third gear 302 through connecting pieces. The second gear ring 303 is provided with three through holes in an annular array, and the three fixing frames 202 in the annular array are all provided with through holes. The limiting shaft 304 is limitedly matched with the through holes on the adjacent second gear ring 303, and the limiting shaft 304 locks the adjacent third gear 302 and the second gear ring 303 into one. The limiting shaft 304 is limitedly matched with the through holes on the adjacent fixing frame 202, and the limiting shaft 304 locks the second gear ring 303 and the fixing frame 202 into one, so that the first gear ring 201 drives the second gear ring 303 to rotate synchronously through the three fixing frames 202 in the annular array.

[0027] When it is necessary to compare the bacterial culture of Yersinia at two temperatures, the staff opens the two covers 4, and takes out the placement trays 8 of the linear arrays in the two culture shells 3 in turn, then snaps the culture dishes into the snap rings on the placement trays 8 in turn, and puts the placement trays 8 into the culture shells 3 again, and then rotates the second gear ring 303, which drives the three third gears 302 of the adjacent annular array to rotate, and the third gear 302 drives the second heating rods 301 at the bottom to rotate synchronously, so that the three second heating rods 301 of the annular array deflect toward the central axis of the adjacent mounting column 7, and this is done until the three second heating rods 301 are far away. One end of the adjacent third gear 302 is located on the central axis of the adjacent mounting column 7, and then the second gear ring 303 is stopped. At this time, the central axis of the through hole on the third gear 302 connecting piece, the central axis of the adjacent through hole on the second gear ring 303 and the central axis of the through hole on the adjacent fixing frame 202 are in a straight line. Then the staff slides the three limiting shafts 304 of the annular array downward along the through holes on the three third gear 302 connecting pieces respectively, and does so until the limiting shafts 304 are inserted into the adjacent through holes on the second gear ring 303 and the through holes on the adjacent fixing frame 202. At this time, the three second heating rods 301 of the annular array are locked.

[0028] When the three second heating rods 301 of the annular array are locked, the staff then rotates the two covers 4 to seal the adjacent culture shells 3 respectively, and then turns on all the first heating rods 203 and the second heating rods 301 in the two culture shells 3 through the control terminal 2, so that the temperatures in the two culture shells 3 reach 24°C and 37°C respectively, and then turns on the two servo motors 5, and the output shafts of the servo motors 5 respectively drive the first gears 6 thereon to rotate, and the first gears 6 drive the adjacent first gear rings 201 to rotate, and the first gear rings 201 drive the three adjacent first heating rods 203 to rotate synchronously through the fixing frame 202 of the annular array, so that the first heating rods 203 rotate around the internal culture dish, and at the same time, the first heating rods 203 drive the second heating rod 301 on the top thereof to rotate synchronously, and a temperature-controlled columnar area is formed by the rotation of the three first heating rods 203 of the annular array and the three second heating rods 301 of the annular array, and several adjacent culture dishes are surrounded to the inside, so that the Yersinia in the culture shell 3 is in a uniform temperature environment, and the external temperature is prevented from being transferred to the culture shell 3, resulting in uneven temperature in the culture shell 3.

[0029] When the output shaft of the servo motor 5 drives the adjacent first gear 6 to rotate, the first gear 6 drives the adjacent second gear 9 to rotate. When the second gear 9 drives the card shaft 10 thereon to rotate synchronously, the card shaft 10 rotates and slides along the adjacent slide groove 11. Since the slide groove 11 is spliced ​​with two spiral grooves, the second gear 9 rotates one circle, causing the mounting column 7 to perform a linear reciprocating motion, and at the same time drives the placement plate 8 of the linear array thereon to perform a linear reciprocating motion synchronously, thereby causing the culture dishes of the annular array on the placement plate 8 to perform a linear reciprocating motion synchronously, thereby increasing the contact between the culture dishes and the external heat, and the cycle is repeated until the cultivation is completed. The present invention drives the culture dishes on the placement plate 8 to perform a linear reciprocating motion up and down, continuously changes the position of the culture dishes, enables them to perform heat exchange with the heat at multiple positions, and avoids the culture dishes from being stationary. When some heating elements are damaged, the temperature in the culture dishes is uneven.

[0030] When the Yersinia culture is completed, turn off the two servo motors 5, and at the same time turn off all the first heating rods 203 and the second heating rods 301, then open the cover 4, and pull out the three limit shafts 304 of the annular array, release the lock of the three third gears 302 and the second gear ring 303 of the annular array, and then the staff rotates the second gear ring 303, and the second gear ring 303 drives the three third gears 302 of the annular array to rotate, so that the third gear 302 drives the adjacent second heating rods 301 to deflect, so that the staff can take out the placement plate 8 of the linear array, then take the culture dish and reset the placement plate 8, and then rotate the cover 4 again to seal the adjacent culture shell 3, and repeat the above steps when Yersinia culture is needed again.

[0031] Embodiment 2: Based on embodiment 1, Figure 5 and Fig. 9As shown, it also includes two push components that are distributed in a mirror image and are used to push the gas inside the adjacent culture shells 3 respectively. The push components include six groups of impact cylinders 401 in an annular array. The six groups of impact cylinders 401 in the annular array are fixedly connected to the bottom of the adjacent culture shells 3 through connecting pieces. Each group includes three impact cylinders 401 in a linear array. The three impact cylinders 401 in the linear array are all sealed and slidably connected with piston plates 402. The three impact cylinders 401 in the linear array are respectively provided with first elastic elements 403 between the adjacent piston plates 402. The first elastic elements 403 are springs. It is used to drive the adjacent piston plates 402 to reset. The impact cylinder 401 and the adjacent piston plates 402 cooperate to form a first closed cavity. A pipeline is fixedly connected between the two adjacent impact cylinders 401. The two adjacent first closed cavities are connected through the pipeline. A driving component for driving the adjacent linear array piston plates 402 to move is provided in the culture shell 3. The piston plate 402 impacts the gas near the first heating rod 203, so that the gas at a suitable temperature can replace the gas at the culture dish in time, ensuring that the gas at the culture dish is always at the set temperature value, thereby improving the culture effect of Yersinia.

[0032] like Figure 5 and Fig. 9 As shown, the driving assembly includes six docking cylinders 404 in a circular array, and the six docking cylinders 404 in the circular array are fixedly connected to the bottom of the culture shell 3 through connecting pieces. The six docking cylinders 404 in the circular array are respectively fixedly connected to adjacent impact cylinders 401 through pipelines. The docking cylinders 404 are sealed and slidably connected with piston shafts 405. The docking cylinders 404 and the adjacent piston shafts 405 cooperate to form a second closed cavity. The second closed cavity is connected to the adjacent first closed cavity through a pipeline, so that the gas in the first closed cavity flows into the second closed cavity through the pipeline. A second elastic element 406 is arranged between the docking cylinder 404 and the adjacent piston shaft 405. The second elastic element 406 is a spring, and the second elastic element 406 is used to drive the adjacent piston shaft 405 to reset. The outside of the first gear ring 201 is fixedly connected with six extrusion blocks 407 in an annular array. The extrusion blocks 407 are right-angled triangle blocks. The six extrusion blocks 407 in the annular array are all extruded and matched with the six piston shafts 405 in the annular array. The hypotenuse of the extrusion block 407 squeezes the adjacent piston shaft 405 to move downward. At this time, the gas in the second closed cavity draws the gas in the three adjacent first closed cavities, so that the piston plate 402 slides along the inside of the adjacent impact cylinder 401, and each group of impact cylinders 401 is provided with a reversing component for changing the pushing direction of the adjacent impact cylinders 401.

[0033] like Fig. 9 and Fig.10As shown, the reversing assembly includes three flexible rings 501 in a linear array, the flexible rings 501 are made of rubber, the three flexible rings 501 in the linear array are respectively fixedly connected to adjacent impact cylinders 401, the flexible rings 501 are fixedly connected to the reversing cylinder 502, the flexible rings 501 are used to change the orientation of the reversing cylinder 502, the three reversing cylinders 502 in the linear array are commonly fixedly connected to an adjusting frame 503, the bottom of the adjusting frame 503 is slidably connected to a sliding rod 504, the sliding rod 504 is fixedly connected to an adjacent piston shaft 405, the piston shaft 405 drives the adjacent sliding rod 504 to move up and down, and a swinging assembly for swinging the adjusting frame 503 is provided on the piston shaft 405.

[0034] like Fig.10 As shown, the swing assembly includes a first block 505, the first block 505 has symmetrically distributed inclined surfaces, the first block 505 is slidably connected to the top of the adjacent sliding rod 504, a third elastic element 506 is arranged between the first block 505 and the adjacent sliding rod 504, the third elastic element 506 is a spring, the third elastic element 506 is used to drive the adjacent first block 505 to reset, and the spring coefficient of the third elastic element 506 is greater than the sum of the elastic coefficients of the three flexible rings 501 of the adjacent linear array, and the adjustment frame 503 is fixedly connected to the three second blocks of the linear array 507, the second clamping block 507 is provided with symmetrically distributed inclined surfaces, and a gap is left between two adjacent second clamping blocks 507, so that the flexible ring 501 can drive the adjacent reversing cylinder 502 to reset. The three second clamping blocks 507 of the linear array are all squeezed and matched with the adjacent first clamping blocks 505, and the piston shaft 405 drives the first clamping block 505 to squeeze the adjacent second clamping block 507, and then drives the three reversing cylinders 502 of the adjacent linear array to continuously change their directions through the adjustment frame 503, thereby increasing the impact range of the piston plate 402 on the gas and further improving the stability of the gas temperature around the culture dish.

[0035] When the first gear ring 201 drives the three adjacent first heating rods 203 to rotate, the first gear ring 201 drives the extrusion blocks 407 in the annular array thereon to rotate synchronously, and the extrusion blocks 407 contact the adjacent piston shaft 405 and squeeze the piston shaft 405. The piston shaft 405 slides downward along the adjacent docking tube 404 under the extrusion force, and at the same time the second elastic element 406 is compressed, so that the volume of the second closed cavity coordinated by the two is increased, and the gas in the three adjacent first closed cavities is drawn into the adjacent second closed cavity along the pipeline. The volume of the first closed cavity is reduced, causing the piston plate 402 inside it to slide inward, and the gas in the adjacent culture shell 3 is drawn into the adjacent impact tube 401. At the same time, the first elastic element 403 is compressed. When the activity When the plug shaft 405 is separated from the adjacent extrusion block 407, the second elastic element 406 is reset and drives the adjacent piston shaft 405 to reset, and the volume of the second closed cavity is restored. At the same time, the gas in the second closed cavity enters the three adjacent first closed cavities along the pipeline, and then the three first elastic elements 403 of the linear array are reset, driving the three piston plates 402 to reset. The reset of the piston plate 402 pushes the gas in the adjacent impact cylinder 401 to impact the gas in the adjacent culture shell 3. This cycle allows the gas near the first heating rod 203 to flow into the culture dish, and the gas at the culture dish is replaced in time with the gas at an appropriate temperature to ensure that the gas at the culture dish is always at the set temperature value, thereby improving the culture effect of Yersinia.

[0036] When the piston shaft 405 is subjected to the downward sliding of the adjacent extrusion block 407, the piston shaft 405 drives the adjacent sliding rod 504 to slide downward synchronously. At this time, the sliding rod 504 drives the first block 505 at its top to slide downward synchronously. At this time, the first block 505 drives the three reversing cylinders 502 of the adjacent linear array to deflect downward through the adjacent second block 507 and the adjusting frame 503. At the same time, the flexible ring 501 bends. When the flexible ring 501 bends to the limit state, the second block 507 squeezes the first block 505, so that the first block 505 slides along the adjacent sliding rod 504 under the extrusion force, and the third elastic element 506 is compressed. Then the first block 505 is separated from the adjacent second block 507. Then the flexible ring 501 drives the adjacent reversing cylinder 502 to reset. The three reversing cylinders 502 of the linear array synchronously drive the adjacent adjusting frame 503 to reset. At the same time, the third elastic element 506 resets and drives the adjacent third The first block 505 is reset, and the piston shaft 405 continues to drive the sliding rod 504 to move downward until it contacts the next second block 507, and repeats the above steps until the piston shaft 405 drives the sliding rod 504 to slide to the limit state. At this time, the piston plate 402 slides to the inside of the adjacent impact cylinder 401, so that the gas in the culture shell 3 enters the impact cylinder 401, and then the piston shaft 405 is separated from the adjacent extrusion block 407, and the piston shaft 405 drives the sliding rod 504 to move upward. The sliding rod 504 drives the first block 505 at the top to contact and squeeze each other with the second block 507 at the bottom, and repeats the above steps so that the piston plate 402 pushes the gas in the adjacent impact cylinder 401 into the culture shell 3, and performs linear reciprocating motion through the three reversing cylinders 502 of the linear array, changes the impact direction of the piston plate 402, increases the impact range of the gas, and further stabilizes the temperature of the gas around the culture dish.

[0037] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A Yersinia culture device with a uniform temperature control function, comprising a base (1), the base (1) being fixedly connected to a control terminal (2), the base (1) being fixedly connected to culture shells (3) distributed in a mirror image, the culture shells (3) distributed in a mirror image being hinged with a cover (4), the culture shells (3) distributed in a mirror image being fixedly connected with a servo motor (5), the servo motors (5) distributed in a mirror image being electrically connected to the control terminal (2), the output shaft of the servo motor (5) being fixedly connected to a first gear (6), characterized in that: The invention also comprises mirror-image-distributed mounting columns (7), wherein the mirror-image-distributed mounting columns (7) are respectively slidably connected to adjacent culture shells (3) via connecting pieces, and the mounting columns (7) are detachably connected to a placement plate (8) in a linear array, and a second gear (9) is rotatably connected to the culture shell (3) via a connecting piece and meshes with the adjacent first gear (6), and the second gear (9) is fixedly connected to a clamping shaft (10), and a slide groove (11) is provided at the bottom of the mounting columns (7), and the clamping shaft (10) is slidably connected to the adjacent slide groove (11), and the mirror-image-distributed culture shells (3) are each provided with a first temperature control component for maintaining a uniform internal temperature.

2. The Yersinia culture device with uniform temperature control function according to claim 1, characterized in that: The radius of the mounting column (7) gradually decreases from the bottom to the top, and the inner circle radius of the placement plate (8) gradually decreases from the bottom to the top, so as to keep the distance between the placement plates (8) of the linear array consistent.

3. The Yersinia culture device with uniform temperature control function according to claim 1, characterized in that: The first temperature control component includes a first gear ring (201), the first gear ring (201) is rotatably connected to the inside of the adjacent culture shell (3), the first gear ring (201) is fixedly connected to a fixing frame (202) of an annular array, the fixing frames (202) of the annular array are all fixedly connected to a first heating rod (203) electrically connected to the control terminal (2), the first gear ring (201) is meshed with the first gear (6), and the first heating rods (203) of the annular array are commonly provided with a second temperature control component.

4. The Yersinia culture device with uniform temperature control function according to claim 3, characterized in that: The second temperature control component comprises a second heating rod (301) in a circular array, the second heating rods (301) in the circular array are all electrically connected to the control terminal (2), the second heating rods (301) in the circular array are respectively rotatably connected to adjacent first heating rods (203), the second heating rods (301) in the circular array are all fixedly connected to a third gear (302), a second gear ring (303) is rotatably connected inside the culture shell (3), the third gears (302) in the circular array are all meshed with adjacent second gear rings (303), and a locking component for locking the third gear (302) is provided on the third gear (302) in the circular array.

5. The Yersinia culture device with uniform temperature control function according to claim 4, characterized in that: The locking assembly comprises a ring array of limiting shafts (304), the ring array of limiting shafts (304) are respectively slidably connected to adjacent third gears (302) through connecting pieces, the second gear ring (303) is provided with a ring array of through holes, the ring array of fixing frames (202) are all provided with through holes, the limiting shafts (304) are limitedly matched with the through holes on the adjacent second gear ring (303), and the limiting shafts (304) are limitedly matched with the through holes on the adjacent fixing frames (202).

6. The Yersinia culture device with uniform temperature control function according to claim 4, characterized in that: The invention also includes a propulsion assembly which is distributed in a mirror image and is used to respectively propel the gas inside adjacent culture shells (3). The propulsion assembly includes a plurality of groups of impact cylinders (401) in an annular array. The plurality of groups of impact cylinders (401) in the annular array are fixedly connected to the inside of adjacent culture shells (3) through connecting pieces. Each group includes a plurality of impact cylinders (401) in a linear array. The impact cylinders (401) in the linear array are sealed and slidably connected with piston plates (402). First elastic elements (403) are respectively arranged between the impact cylinders (401) in the linear array and the adjacent piston plates (402). The impact cylinders (401) and the adjacent piston plates (402) cooperate to form a first closed cavity. A pipeline is fixedly connected between two adjacent impact cylinders (401). The two adjacent first closed cavities are connected through the pipeline. A driving assembly for driving the adjacent linear array piston plates (402) to move is arranged in the culture shell (3).

7. The Yersinia culture device with uniform temperature control function according to claim 6, characterized in that: The driving assembly comprises a circular array of docking cylinders (404), which are fixedly connected to the interior of the culture shell (3) through connecting pieces, and the docking cylinders (404) of the circular array are fixedly connected to adjacent impact cylinders (401) through pipelines, and the interior of the docking cylinder (404) is sealed and slidably connected with a piston shaft (405), and the docking cylinder (404) and the adjacent piston shaft (405) cooperate to form a second closed cavity, and the second closed cavity is connected to the adjacent first closed cavity through a pipeline, and a second elastic element (406) is arranged between the docking cylinder (404) and the adjacent piston shaft (405), and the outside of the first gear ring (201) is fixedly connected with an annular array of extrusion blocks (407), and the annular array of extrusion blocks (407) are extruded and matched with the annular array of piston shafts (405), and each group of the impact cylinders (401) is provided with a reversing assembly for changing the pushing direction of adjacent impact cylinders (401).

8. The Yersinia culture device with uniform temperature control function according to claim 6, characterized in that: The reversing assembly comprises a linear array of flexible rings (501), the flexible rings (501) of the linear array are respectively fixedly connected to adjacent impact cylinders (401), the flexible rings (501) are fixedly connected to reversing cylinders (502), the reversing cylinders (502) of the linear array are commonly fixedly connected to an adjustment frame (503), the adjustment frame (503) is slidably connected to a sliding rod (504), the sliding rod (504) is fixedly connected to an adjacent piston shaft (405), and a swinging assembly for swinging the adjustment frame (503) is provided on the piston shaft (405).

9. The Yersinia culture device with uniform temperature control function according to claim 8, characterized in that: The swing assembly comprises a first clamping block (505), the first clamping block (505) is slidably connected to an adjacent sliding rod (504), a third elastic element (506) is arranged between the first clamping block (505) and the adjacent sliding rod (504), the adjustment frame (503) is fixedly connected to a second clamping block (507) of a linear array, a gap is left between two adjacent second clamping blocks (507), and the second clamping blocks (507) of the linear array are all squeezed and matched with the adjacent first clamping blocks (505).

10. The Yersinia culture device with uniform temperature control function according to claim 9, characterized in that: The spring constant of the third elastic element (506) is greater than the sum of the spring constants of the flexible rings (501) in adjacent linear arrays.