Anaerobic ammonia oxidizing bacteria growth promoter as well as preparation method and application thereof

Abstract

The invention discloses an anaerobic ammonia oxidizing bacteria growth promoter as well as a preparation method and application thereof. The anaerobic ammonia oxidizing bacteria growth promoter is prepared from the following components in parts by weight: 30 to 50 parts of formate, 3 to 5 parts of ferrite, 1 to 3 parts of copper salt, 0 to 15 parts of calcium salt, 5 to 10 parts of activated carbon and 10 to 30 parts of adhesive. The preparation method comprises the following steps: weighing the components according to the formula, uniformly mixing, adding 5-20 parts by weight of water, uniformly stirring to obtain a viscous paste, granulating, and drying to obtain the anaerobic ammonia oxidizing bacteria growth promoter. The growth promoter provided by the invention not only can rapidly and stably promote the growth of anaerobic ammonia oxidizing bacteria, but also has the advantages of high safety and no secondary pollution.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and specifically relates to an anaerobic ammonia oxidizing bacteria growth promoter and a preparation method and application thereof. Background Art

[0002] Anaerobic ammonium oxidation technology is a new type of biological denitrification technology that uses anaerobic microorganisms to convert ammonia nitrogen and nitrite into nitrogen gas under anaerobic conditions, thereby achieving efficient nitrogen removal. This technology has received widespread attention in the field of wastewater treatment in recent years and is regarded as a powerful alternative to traditional nitrification-denitrification processes. Its unique reaction mechanism not only saves energy consumption, but also reduces the demand for organic carbon sources, and has significant economic and environmental benefits.

[0003] However, due to the slow growth rate of anammox bacteria, it usually takes several months or even longer to start a new anammox system, which poses a challenge to practical applications. In addition, the microbial community in the anammox system is complex. In addition to anammox bacteria, there may be other facultative or heterotrophic microorganisms. The growth rate of these microorganisms is usually an order of magnitude higher than that of anammox bacteria, and thus they will compete with anammox bacteria for substrates and space. Therefore, promoting the growth of anammox bacteria is conducive to maintaining the stability and efficiency of the anammox process.

[0004] CN106754618 B discloses a method for enriching and culturing anaerobic ammonia-oxidizing bacteria, which adds a growth promoter to the culture solution and cooperates with the process conditions to achieve efficient enrichment of anaerobic ammonia-oxidizing bacteria. The growth promoter includes metal salts, polyamine substances, inorganic acid hydroxylamine and Na2SO3. However, the promoter needs to be added continuously and may not be fully utilized by microorganisms, and then will flow into the receiving water body with the effluent, causing secondary pollution. Summary of the invention

[0005] Purpose of the invention: The purpose of the present invention is to provide an anaerobic ammonium oxidizing bacteria growth promoter and its preparation method and application. The anaerobic ammonium oxidizing bacteria growth promoter of the present invention has a simple formula, low price, easy preparation, and a sustained release function, and can quickly and stably promote the growth of anaerobic ammonium oxidizing bacteria.

[0006] Technical solution: The purpose of the present invention is achieved through the following technical solution:

[0007] The present invention provides an anaerobic ammonium oxidizing bacteria growth promoter, which is composed of the following components in parts by weight:

[0008] 30-50 parts by weight of formate, 3-5 parts by weight of ferrous salt, 1-3 parts by weight of copper salt, 0-15 parts by weight of calcium salt, 5-10 parts by weight of activated carbon, and 10-30 parts by weight of binder.

[0009] Preferably, the formate is one or more of calcium formate, sodium formate or ammonium formate. The formate contained in the growth promoter of the present invention can be used as a carbon source required for the growth of anaerobic ammonium oxidizing bacteria, thereby promoting the rapid growth of anaerobic ammonium oxidizing bacteria.

[0010] Preferably, the ferrous salt is selected from one or both of ferrous sulfate and ferrous chloride.

[0011] Preferably, the copper salt is selected from one or both of copper sulfate and copper chloride.

[0012] Preferably, the calcium salt is selected from one or both of calcium chloride and calcium sulfate.

[0013] The present invention utilizes metal ions provided by ferrous salts and copper salts as trace elements required for the growth of anaerobic ammonium oxidizing bacteria, and utilizes calcium ions provided by calcium salts to promote the granulation of anaerobic ammonium oxidizing bacteria.

[0014] Preferably, the particle size of the activated carbon is ≥100 mesh, which can provide a larger growth platform for anaerobic ammonia-oxidizing bacteria and is easy to settle, reducing loss.

[0015] Preferably, the adhesive is one or more of sodium alginate, polyvinyl alcohol, chitosan or sodium polyacrylate. The adhesive selected in the present invention has the characteristics of low biological toxicity and stable properties. The present invention adds an adhesive to shape the drug and achieve a sustained release effect, otherwise the drug will dissolve in water and will quickly drain.

[0016] The present invention also provides a method for preparing the anaerobic ammonium oxidizing bacteria growth promoter, comprising the following steps:

[0017] Formate, ferrous salt, copper salt, calcium salt, activated carbon and adhesive are mixed in proportion, 5 to 20 parts by weight of water are added, stirred evenly to a viscous paste, granulated and dried to prepare the anaerobic ammonia oxidizing bacteria growth promoter.

[0018] A preferred embodiment of the present invention is that the particle size of the granulation is 2-10 mm; the drying includes two stages: the first stage is 50-60°C and the drying time is 2-3 hours; the second stage is 30-40°C and the drying time is 4-6 hours.

[0019] Furthermore, the drying is carried out in a vacuum drying oven.

[0020] The present invention also provides application of the anaerobic ammonium oxidizing bacteria growth promoter in the anaerobic ammonium oxidizing process.

[0021] During use, the concentration of the anaerobic ammoniation bacteria growth promoter is 100-500 g / L, and it is directly added to the reactor containing the anaerobic ammonium oxidation sludge, or loaded into the bottom of the anaerobic ammonium oxidation reactor as a filler layer.

[0022] Beneficial effects:

[0023] (1) The growth promoter of the present invention has a sustained release function. After being added once, it does not need to be added again within six months.

[0024] (2) After the biochemical promoter of the present invention was added to the anaerobic ammonium oxidation reactor, the chemical oxygen demand and metal ion concentration in the effluent were not significantly different from those of the control group without the biochemical promoter, and no secondary pollution was caused. DETAILED DESCRIPTION

[0025] The technical solution of the present invention is described in detail below through specific embodiments, but the protection scope of the present invention is not limited to the embodiments.

[0026] Example 1 Preparation of anaerobic ammonium oxidizing bacteria growth promoter

[0027] Formate, ferrous salt, copper salt, calcium salt, activated carbon and adhesive are mixed in proportion, 15 parts by weight of water are added, and the mixture is stirred for 5 minutes until a viscous paste is formed. The mixture is then put into a granulation mold with a particle size of 10 mm for granulation. The granulation mold is placed in a vacuum drying oven, dried at 60°C for 2 hours, and then dried at 40°C for 4 hours to prepare an anaerobic ammonia-oxidizing bacteria growth promoter.

[0028] The formula of the anaerobic ammonium oxidizing bacteria growth promoter is shown in Table 1.

[0029] Example 2 Preparation of anaerobic ammonium oxidizing bacteria growth promoter

[0030] Formate, ferrous salt, copper salt, calcium salt, activated carbon and adhesive are mixed in proportion, 20 parts by weight of water are added, and the mixture is stirred for 5 minutes until a viscous paste is formed. The mixture is then put into a granulation mold with a particle size of 10 mm for granulation. The granulation mold is placed in a vacuum drying oven, dried at 60°C for 2 hours, and then dried at 30°C for 6 hours to prepare an anaerobic ammonia-oxidizing bacteria growth promoter.

[0031] The formula of the anaerobic ammonium oxidizing bacteria growth promoter is shown in Table 1.

[0032] Example 3 Preparation of anaerobic ammonium oxidizing bacteria growth promoter

[0033] Formate, ferrous salt, copper salt, calcium salt, activated carbon and adhesive are mixed in proportion, 10 parts by weight of water are added, and the mixture is stirred for 5 minutes until a viscous paste is formed. The mixture is then put into a granulation mold with a particle size of 5 mm for granulation. The granulation mold is placed in a vacuum drying oven, dried at 50°C for 3 hours, and then dried at 40°C for 4 hours to prepare an anaerobic ammonia-oxidizing bacteria growth promoter.

[0034] The formula of the anaerobic ammonium oxidizing bacteria growth promoter is shown in Table 1.

[0035] Comparative Example 1

[0036] Ferrous salt, copper salt, calcium salt, activated carbon and adhesive are mixed in proportion, 5 parts by weight of water are added, and the mixture is stirred for 5 minutes until a viscous paste is formed. The mixture is then put into a granulation mold with a particle size of 10 mm for granulation. The granulation mold is placed in a vacuum drying oven, dried at 60°C for 2 hours, and then dried at 40°C for 4 hours to prepare an anaerobic ammonia-oxidizing bacteria growth promoter.

[0037] The formula of the anaerobic ammonium oxidizing bacteria growth promoter is shown in Table 1.

[0038] Table 1 Formula of anaerobic ammonium oxidizing bacteria growth promoter

[0039]

[0040] Application Examples

[0041] The anaerobic ammonium oxidation sludge from the anaerobic ammonium oxidation denitrification process of coal gasification wastewater in the comprehensive industrial wastewater treatment center of Lianyungang Petrochemical Base, Jiangsu Province was used as the inoculum and inoculated into two upflow anaerobic sludge blanket reactors with an effective volume of 2L respectively. The sludge concentration was controlled at 10gVSS / L.

[0042] Among them, one reactor was not added with anaerobic ammonium-oxidizing bacteria growth promoter, which served as the control group;

[0043] In another reactor, 100 g / L of the anaerobic ammonia-oxidizing bacteria growth promoter prepared in the examples and comparative examples was added to serve as an experimental group.

[0044] The concentrations of ammonia nitrogen and nitrite nitrogen in the reactor influent were 200 mg / L and 260 mg / L, respectively, and the reaction temperature was maintained at 35±2°C. After 60 days of cultivation, an appropriate amount of sludge was taken to extract DNA, and qPCR absolute quantification of the 16S rRNA gene of anaerobic ammonia-oxidizing bacteria was performed (Agrawal S et al. Time to Act-Assessing Variations in QPCR Analyses in Biological Nitrogen Removal with Examples from Partial Nitritation / Anammox Systems [J]. Water Research, 2021, 190: 116604) to obtain the abundance of the 16S rRNA gene of anaerobic ammonia-oxidizing bacteria in the sludge. The specific results are shown in Table 2.

[0045] Table 2 Abundance of 16S rRNA genes of anaerobic ammonium-oxidizing bacteria

[0046]

[0047] From the data in Table 2, after adding the growth promoter of the present invention, the growth rate of the abundance of the 16S rRNA gene of the anaerobic ammonium oxidizing bacteria in Examples 1 to 3 increased by more than 40% compared with the control group. It can be seen that the addition of the growth promoter of the present invention significantly promoted the growth of anaerobic ammonium oxidizing bacteria.

[0048] At the same time, since no formate was added in Comparative Example 1, the growth rate of the abundance of the 16S rRNA gene of the anaerobic ammonium oxidizing bacteria therein was reduced by 32% compared with that in Example 3. It can be seen that in the absence of formate, the prepared growth promoter has little effect on promoting the growth of anaerobic ammonium oxidizing bacteria.

[0049] In addition, in the experimental group, the total mass change of the growth promoter added in each example was within 20% after 60 days of reaction. It can be seen that the growth promoter prepared by the present invention has high stability and sustained release.

[0050] As described above, although the present invention has been shown and described with reference to specific preferred embodiments, it should not be construed as limiting the present invention itself. Various changes in form and details may be made without departing from the spirit and scope of the present invention as defined in the appended claims.

Claims

1. An anaerobic ammonium oxidizing bacteria growth promoter, characterized in that: In parts by weight, its composition is as follows: 30-50 parts by weight of formate, 3-5 parts by weight of ferrous salt, 1-3 parts by weight of copper salt, 0-15 parts by weight of calcium salt, 5-10 parts by weight of activated carbon, and 10-30 parts by weight of binder.

2. The anaerobic ammonium oxidizing bacteria growth promoter according to claim 1, characterized in that The formate is one or more of calcium formate, sodium formate or ammonium formate.

3. The anaerobic ammonium oxidizing bacteria growth promoter according to claim 1, characterized in that The ferrous salt is selected from one or both of ferrous sulfate and ferrous chloride.

4. The anaerobic ammonium oxidizing bacteria growth promoter according to claim 1, characterized in that The copper salt is selected from one or both of copper sulfate and copper chloride.

5. The anaerobic ammonium oxidizing bacteria growth promoter according to claim 1, characterized in that The calcium salt is selected from one or both of calcium chloride and calcium sulfate.

6. The anaerobic ammonium oxidizing bacteria growth promoter according to claim 1, characterized in that The particle size of the activated carbon is ≥100 meshes.

7. The anaerobic ammonium oxidizing bacteria growth promoter according to claim 1, characterized in that: The adhesive is one or more of sodium alginate, polyvinyl alcohol, chitosan or sodium polyacrylate.

8. The method for preparing the anaerobic ammonium oxidizing bacteria growth promoter according to any one of claims 1 to 7, characterized in that: The following steps are involved: Formate, ferrous salt, copper salt, calcium salt, activated carbon and adhesive are mixed in proportion, 5 to 20 parts by weight of water are added, stirred evenly to a viscous paste, granulated and dried to prepare the anaerobic ammonia oxidizing bacteria growth promoter.

9. The preparation method according to claim 8, characterized in that: The particle size of the granulation is 2-10 mm; the drying includes two stages: the first stage is 50-60° C., and the drying time is 2-3 hours; the second stage is 30-40° C., and the drying time is 4-6 hours.

10. Use of the anaerobic ammonium oxidizing bacteria growth promoter according to any one of claims 1 to 7 in an anaerobic ammonium oxidation process, characterized in that: During use, the concentration of the anaerobic ammoniation bacteria growth promoter is 100-500 g / L, and it is directly added to the reactor containing the anaerobic ammonium oxidation sludge, or loaded into the bottom of the anaerobic ammonium oxidation reactor as a filler layer.

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