Method for quantitatively judging typical wetland plants

Abstract

The invention discloses a method for quantitatively judging typical wetland plants, belongs to the technical field of wetland science, and relates to a method for judging typical wetland plants. The invention aims to solve the problem that a complete typical wetland plant species catalog is difficult to form due to the absence of a method for judging typical wetland plants at present. The method comprises the six steps of target establishment, sample collection, seed treatment, seed sowing, water management and type judgment. And judging whether the target plant is a typical wetland plant or not by comparing the emergence rate and the seedling growth height of the seeds under the waterflooding and moist conditions. The determination method can objectively reflect the actual condition that the plant adapts to moisture, can treat multiple species at the same time, has low requirements on equipment, is simple to operate, is scientific, and is suitable for large-scale application.

Description

Technical Field

[0001] The invention belongs to the field of wetland science and technology, and particularly relates to a method for determining typical wetland plants. Background Art

[0002] Plants are divided into xerophytes, mesophytes, hygrophytes and aquatic plants according to their adaptability to water conditions. Aquatic plants and hygrophytes only appear in hygrophytic environments and are typical wetland plants. Some plants can appear in both wetlands and non-wetlands in the same area, or only in wetlands in some areas, but in wetlands or non-wetlands in other areas. These plants are hygrophytes among mesophytes and also belong to wetland plants. Mesophytes have a wide ecological range of adapting to water changes, which makes it difficult to distinguish whether they are typical wetland plants in actual operations. This is also an important reason why different scholars disagree on whether the same species belongs to wetland plants. The establishment of a list of typical wetland plant species is an important prerequisite for conducting regional wetland resource surveys, wetland demarcation, wetland ecological function evaluation and other work. However, there is currently no effective method for determining typical wetland plants, making it difficult to form a complete list of typical wetland plant species, which is not conducive to government management departments and scientific research institutions to carry out relevant work in the field of wetlands. Summary of the invention

[0003] The purpose of the present invention is to solve the technical problem that there is currently no method for identifying typical wetland plants and it is difficult to form a complete list of typical wetland plant species, and to provide a method for quantitatively identifying typical wetland plants.

[0004] The method for quantitatively determining typical wetland plants is as follows:

[0005] 1. Target establishment: Determine the distribution area and suspected plant species according to the research objectives;

[0006] 2. Sample collection: Collect seeds of target plants in the distribution area, air-dry them in a cool place, remove impurities, and check whether the seeds are dormant; dig 20 cm of soil from the surface of the seed collection site, air-dry it, remove roots and stones, grind it, and pass it through a 2 mm sieve for later use;

[0007] 3. Seed treatment: After the seeds treated in step 2 are sterilized, they are stored in a low temperature and wet place for 30-120 days to promote seed germination;

[0008] 4. Seed sowing: Evenly spread 10-80 seeds processed in step 2 or step 3 in a plastic cup containing the soil matrix obtained in step 2, requiring the surface of the soil matrix to be flat and 5 cm from the upper edge of the cup, and then cover the seeds with 0.2-0.5 cm of soil matrix;

[0009] 5. Water management: quickly place the culture cup in step 4 in a large culture tray, add water to achieve flooding and moistening treatment; add water every day, and record the emergence of seedlings. After 10-30 days, randomly select 5 plants to measure the average plant height, and measure all plants with less than 5 plants;

[0010] The flooding treatment means that the water level is 2 cm above the soil substrate surface, and the moistening treatment means that the soil substrate is kept moist 4 cm below the soil substrate surface;

[0011] 6. Type determination: Determine the plant type based on the seed germination rate and average plant height under the two water treatments in step 5;

[0012] The determination is specifically:

[0013] If the plant emergence rate under flooded conditions is greater than or equal to 75% of that under wet conditions, and the plant height is greater than or equal to the value under wet conditions, it is a typical wetland plant (condition 1);

[0014] If the plant emergence rate under flooded conditions is greater than or equal to 75% of that under wet conditions, but the plant height is significantly less than the value under wet conditions, it is a mesophyte; mesophytes may occasionally appear in wetlands, but they are not typical wetland plants (condition 2);

[0015] If the plant emergence rate under flooded conditions is less than 75% of that under wet conditions, and the plant height is significantly lower than that under wet conditions, it is a xerophyte and is not a typical wetland plant (condition 3).

[0016] The area described in step 1 is an administrative level or a geographical division. The administrative levels include provinces and cities, and the geographical divisions include mountainous areas, plains, and plateaus.

[0017] The lifespan of the seeds described in step 2 should be at least more than 1 month.

[0018] The low temperature and wet storage described in step three is only for dormant seeds.

[0019] The plastic cup described in step 4 has a diameter of 10 cm and a height of 20 cm, and has a small hole with a diameter of 1 mm at the bottom to allow water to enter.

[0020] The number of seeds in step 4 is determined according to the size of the seeds; the smaller the seeds and the greater the number, the less soil matrix is ​​required to cover them.

[0021] The duration of the experiment described in step 5 is determined according to the emergence of new seedlings. If no new seedlings emerge for 7 consecutive days, the experiment is considered to be over.

[0022] The present invention has the following advantages:

[0023] (1) Following the objective law that "the seedling stage is the key stage in the life history of plants and is most sensitive to environmental changes", it is concluded that only two indicators are used to quantitatively determine whether a species is a typical wetland plant. The method is simple and effective;

[0024] (2) The present invention is created on the basis of a large number of plant seedling renewal studies, and can objectively reflect the actual situation of plant adaptation to water, and the method is scientific;

[0025] (3) It can test multiple species simultaneously, has low requirements for instruments and equipment, is easy to operate, and is suitable for large-scale application. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a comparison chart of seed emergence rates under different water conditions in Examples 1 to 4;

[0027] Figure 2 It is a comparison chart of seedling heights under different moisture conditions in Examples 1 to 4. DETAILED DESCRIPTION

[0028] The technical solution of the present invention is not limited to the specific implementation modes listed below, but also includes any combination of the specific implementation modes.

[0029] Specific implementation method 1: The method for quantitatively determining typical wetland plants in this implementation method is as follows:

[0030] 1. Target establishment: Determine the distribution area and suspected plant species according to the research objectives;

[0031] 2. Sample collection: Collect seeds of target plants in the distribution area, air-dry them in a cool place, remove impurities, and check whether the seeds are dormant; dig 20 cm of soil from the surface of the seed collection site, air-dry it, remove roots and stones, grind it, and pass it through a 2 mm sieve for later use;

[0032] 3. Seed treatment: After the seeds treated in step 2 are sterilized, they are stored in a low temperature and wet place for 30-120 days to promote seed germination;

[0033] 4. Seed sowing: Evenly spread 10-80 seeds processed in step 2 or step 3 in a plastic cup containing the soil matrix obtained in step 2, requiring the surface of the soil matrix to be flat and 5 cm from the upper edge of the cup, and then cover the seeds with 0.2-0.5 cm of soil matrix;

[0034] 5. Water management: quickly place the culture cup in step 4 in a large culture tray, add water to achieve flooding and moistening treatment; add water every day, and record the emergence of seedlings. After 10-30 days, randomly select 5 plants to measure the average plant height, and measure all plants with less than 5 plants;

[0035] The flooding treatment means that the water level is 2 cm above the soil substrate surface, and the moistening treatment means that the soil substrate is kept moist 4 cm below the soil substrate surface;

[0036] 6. Type determination: Determine the plant type based on the seed germination rate and average plant height under the two water treatments in step 5;

[0037] The determination is specifically:

[0038] If the plant emergence rate under flooded conditions is greater than or equal to 75% of that under wet conditions, and the plant height is greater than or equal to the value under wet conditions, it is a typical wetland plant (condition 1);

[0039] If the plant emergence rate under flooded conditions is greater than or equal to 75% of that under wet conditions, but the plant height is significantly less than the value under wet conditions, it is a mesophyte; mesophytes may occasionally appear in wetlands, but they are not typical wetland plants (condition 2);

[0040] If the plant emergence rate under flooded conditions is less than 75% of that under wet conditions, and the plant height is significantly lower than that under wet conditions, it is a xerophyte and is not a typical wetland plant (condition 3).

[0041] Specific implementation method 2: This implementation method is different from specific implementation method 1 in that the region described in step 1 is an administrative level or a geographical division, the administrative level includes provinces and cities, and the geographical division includes mountainous areas, plains, and plateaus. The rest is the same as specific implementation method 1.

[0042] Specific implementation method 3: This implementation method is different from specific implementation method 1 or 2 in that the life span of the seeds in step 2 should be at least 1 month. The rest is the same as specific implementation method 1 or 2.

[0043] Specific implementation method 4: This implementation method is different from specific implementation methods 1 to 3 in that the low temperature and wet storage in step 3 is only for dormant seeds. The rest is the same as specific implementation methods 1 to 3.

[0044] Specific implementation 5: This implementation is different from Specific implementations 1 to 4 in that the diameter of the plastic cup in step 4 is 10 cm, the height is 20 cm, and there is a small hole with a diameter of 1 mm at the bottom to allow water to enter. The rest is the same as Specific implementations 1 to 4.

[0045] Specific implementation method 6: This implementation method is different from specific implementation methods 1 to 5 in that the number of seeds in step 4 is determined according to the size of the seeds; the smaller the seeds, the greater the number, and the less soil matrix is ​​covered. The rest is the same as specific implementation methods 1 to 5.

[0046] Specific implementation method 7: This implementation method is different from specific implementation methods 1 to 6 in that the duration of the experiment in step 5 is determined according to the emergence of seedlings, and the experiment is considered to be over if no new seedlings emerge for 7 consecutive days. The rest is the same as specific implementation methods 1 to 6.

[0047] The following examples are used to verify the effects of the present invention:

[0048] Example 1

[0049] Alisma plantago-aquatica belongs to the genus Alisma in the family Alismataceae. The tuber diameter is 1-3.5 cm. The leaves are usually numerous; the submerged leaves are linear, the emergent leaves are broadly lanceolate, the base is broadly cuneate and shallowly cordate, and the leaf veins are usually 5. The scape is 78-100 cm high, the inflorescence is 15-50 cm long, with 3-8 whorls of branches, each whorl has 3-9 branches. The flowers are bisexual, with two whorls of tepals, and the inner whorl of tepals is much larger than the outer whorl. The achenes are elliptical, and the fruit beak extends from the ventral side. The seeds are purple-brown.

[0050] Method for quantitatively determining typical wetland plants (determine whether it is a typical wetland plant by analyzing it through the following steps):

[0051] 1. Target establishment: Take Alisma orientalis, which is widely distributed in the Sanjiang Plain, as the target species to detect whether it is a typical wetland plant.

[0052] 2. Sample collection: In mid-to-late August, mature seeds of Alisma orientalis were collected from the riverside zone, lakeside zone, and shallow water of ditches in the Sanjiang Plain. After air-drying in a cool place, impurities were removed and seeds were checked for dormancy. The top 20 cm soil was dug from the seed collection site, air-dried, roots and stones were removed, and the seeds were ground and sieved through a 2 mm sieve for later use.

[0053] 3. Seed treatment: After the seeds treated in step 2 are disinfected with 75% alcohol, they are stored at low temperature and humidity for 60 days to promote seed germination.

[0054] 4. Seed sowing: Evenly spread 30 seeds processed in step 3 in a plastic cup containing the soil matrix obtained in step 2 (the plastic cup has a diameter of 10 cm, a height of 20 cm, and a small hole of 1 mm in diameter at the bottom to allow water to enter). The surface of the matrix should be flat and 5 cm away from the upper edge of the cup. Then cover the seeds with 0.4 cm of soil matrix.

[0055] 5. Water management: quickly place the culture cup in step 4 in a large culture tray and add appropriate amount of water to achieve flooding and moistening. Add water every day and record the emergence of seedlings. After 30 days, randomly select 5 plants to measure the average plant height. If there are less than 5 plants, measure all of them.

[0056] The flooding treatment means that the water level is 2 cm above the soil substrate surface, and the moistening treatment means that the soil substrate is kept moist 4 cm below the soil substrate surface;

[0057] 6. Type determination: Determine the plant type based on the seed germination rate and average plant height under the two water treatments.

[0058] Depend on Figure 1 It can be seen that the germination rate of Alisma orientalis seeds in the wet treatment is only 4%, while the germination rate under 2 cm flooding is as high as 50%, which is 12.5 times that of the wet treatment and much higher than the value under the wet treatment. Figure 2 It can be seen that 30 days after the emergence of Alisma orientalis seeds in the wet treatment, the average plant height was only 4 cm, and the average plant height was 14 cm under 2 cm of flooding, which is also much higher than the value under the wet treatment, satisfying condition 1 (if the plant emergence rate under flooding conditions is greater than or equal to 75% of that under wet conditions, and the plant height is greater than or equal to the value under wet conditions). Therefore, Alisma orientalis is determined to be a typical wetland plant.

[0059] Verification Example 1

[0060] According to the Flora of China, Alisma orientalis grows in shallow waters of lakes, river bends, streams, ponds, swamps, ditches and low-lying wetlands; the Northeast Plant Index describes Alisma orientalis growing in wetlands and watersides. The Wild Vascular Plants of Northeast China Wetlands records that the habitat of Alisma orientalis is: growing in swamps, ditches and watersides. It can be seen that the three authoritative botanical monographs all show that the habitat of Alisma orientalis is wet, and it is a typical wetland plant. This is consistent with the conclusion of Example 1, indicating that the result of judging that Alisma orientalis belongs to a typical wetland plant by this method is reliable.

[0061] Example 2

[0062] Cyperus glomeratus belongs to the genus Cyperus of the family Cyperaceae, and is an annual herb. The culm is 50-95 cm tall and obtusely triangular. The leaf width is 4-8 mm. There are 3-4 leaf-like bracts, which are longer than the inflorescence; the compound long-branched cymes have 3-8 radiating branches, which are of varying lengths, up to 12 cm long; the spike inflorescence is 1-3 cm long, with a very large number of spikelets; the spikelets are multi-row, very densely arranged, with 8-16 flowers; there are 3 stamens and 3 stigmas. The nutlets are oblong and triangular.

[0063] Method for quantitatively determining typical wetland plants (analyzing whether they are typical wetland plants through the following steps):

[0064] 1. Target establishment: Take Cyperus capitata, which is widely distributed in the Sanjiang Plain, as the target species to detect whether it is a typical wetland plant.

[0065] 2. Sample collection: In mid-to-late August, mature seeds of Cyperus capitata were collected from the waterside and ditches of the Sanjiang Plain. After air-drying in a cool place, impurities were removed and the seeds were checked for dormancy. The top 20 cm of soil was dug from the seed collection site, air-dried, roots and stones were removed, and the seeds were ground and sieved through a 2 mm sieve for later use.

[0066] 3. Seed treatment: After the seeds treated in step 2 are disinfected with 75% alcohol, they are stored at low temperature and humidity for 60 days to promote seed germination.

[0067] 4. Seed sowing: Evenly spread 50 seeds treated in step 3 in a plastic cup filled with soil obtained in step 2 (the plastic cup has a diameter of 10 cm, a height of 20 cm, and a small hole of 1 mm in diameter at the bottom to allow water to enter). The surface of the substrate should be flat and 5 cm away from the upper edge of the cup. Then cover the seeds with 0.3 cm of soil substrate.

[0068] 5. Water management: quickly place the culture cup in step 4 in a large culture tray, add appropriate amount of water, and achieve flooding (the flooding means the water level is 2 cm above the soil substrate surface) and moistening treatment (the moistening treatment means keeping the soil substrate 4 cm below the surface moist). Replenish water every day, record the emergence of seedlings, and randomly select 5 plants after 30 days to calculate the average plant height.

[0069] 6. Type determination: Determine the plant type based on the seed germination rate and average plant height under the two water treatments.

[0070] Depend on Figure 1 It can be seen that the germination rate of Cyperus capitata seeds in the wet treatment was 21%, and the germination rate under 2 cm flooding was 17% (81% in the wet treatment, greater than 75%). Figure 2 It can be seen that the average plant height of Cyperus capitatus seeds was only 2 cm after germination in the wet treatment for 30 days, and the average plant height reached 10 cm under 2 cm flooding, which is 5 times that of the wet treatment. It can be seen that although the germination rate of Cyperus capitatus seeds in the 2 cm flooding treatment was slightly lower than that in the wet treatment (but greater than 75% of the wet treatment), and the seedling height was much greater than the value under the wet treatment, it met condition 1 (if the plant germination rate under flooding conditions is greater than or equal to 75% under wet conditions, and the plant height is greater than or equal to the value under wet conditions), therefore, Cyperus capitatus was determined to be a typical wetland plant.

[0071] Verification Example 2

[0072] According to the Flora of China, Cyperus capitatus grows on sandy soil near water or in damp grass beside the road; the Northeast Plant Index describes Cyperus capitatus growing in meadows and paddy fields. The Wild Vascular Plants of Northeast China Wetlands records that the habitat of Cyperus capitatus is: rice fields and riverbank wetlands. It can be seen that, on the whole, the three authoritative botanical monographs describe the habitat of Cyperus capitatus as wetland, which is a typical wetland plant. This is consistent with the conclusion of Example 2, indicating that the result of determining that Cyperus capitatus belongs to a typical wetland plant by this method is reliable.

[0073] Example 3

[0074] Cirsium pendulum belongs to the genus Cirsium of the Asteraceae family and is a perennial herb. The plant grows tall, up to 1 to 3 meters tall, with clustered, upright stems covered with thorns; the stem leaves are 4 to 16 cm long and 1.5 to 6 cm wide; the flower heads are drooping.

[0075] Method for quantitatively determining typical wetland plants (analyzing whether they are typical wetland plants through the following steps):

[0076] 1. Target establishment: Take the tobacco thistle distributed in the Sanjiang Plain as the target species to detect whether it is a typical wetland plant.

[0077] 2. Sample collection: In mid-to-late August, mature seeds of Tobacco Thistle were collected from riverbanks, meadows, and swamps in the Sanjiang Plain. Impurities were removed after air-drying in a cool place. Inspection showed that some seeds were dormant. The top 20 cm of soil was dug from the seed collection site, air-dried, roots and stones were removed, and the seeds were ground and sieved through a 2 mm sieve for later use.

[0078] 3. Seed treatment: After the seeds treated in step 2 are disinfected with 75% alcohol, they are stored at low temperature and wet for 30 days to promote seed germination.

[0079] 4. Seed sowing: Evenly spread 50 seeds treated in step 3 in a plastic cup filled with soil obtained in step 2 (the plastic cup has a diameter of 10 cm, a height of 20 cm, and a small hole of 1 mm in diameter at the bottom to allow water to enter). The surface of the substrate should be flat and 5 cm away from the upper edge of the cup. Then cover the seeds with 0.3 cm of soil substrate.

[0080] 5. Water management: quickly place the culture cup in step 4 in a large culture tray, add appropriate amount of water, and achieve flooding (the flooding means the water level is 2 cm above the soil substrate surface) and moistening treatment (the moistening treatment means keeping the soil substrate 4 cm below the surface moist). Replenish water every day, record the emergence of seedlings, and randomly select 5 plants after 30 days to calculate the average plant height.

[0081] 6. Type determination: Determine the plant type based on the seed germination rate and average plant height under the two water treatments.

[0082] Depend on Figure 1 It can be seen that the germination rate of tobacco thistle seeds in the wet treatment was 57%, and the germination rate under 2 cm flooding was 49% (86% in the wet treatment, greater than 75%); Figure 2 It can be seen that the average plant height of tobacco thistle seeds was 20 cm after germination 30 days after the wet treatment, and the average plant height was only 5 cm under 2 cm flooding, which is 25% of the wet treatment. It can be seen that although the germination rate of tobacco thistle seeds under 2 cm flooding treatment is slightly higher than 75% of the wet treatment value, the seedling height is much smaller than the value under the wet treatment, which meets condition 2 (if the plant germination rate under flooding conditions is greater than or equal to 75% under wet conditions, but the plant height is significantly smaller than the value under wet conditions, it is a mesophyte; mesophytes may occasionally appear in wetlands, but they are not typical wetland plants). Therefore, tobacco thistle is determined to be a mesophyte, although it can occasionally appear in natural wetlands, it is not a typical wetland plant.

[0083] Verification Example 3

[0084] According to the Flora of China, tobacco thistle grows on riverbanks, grasslands, and forest edges on hillsides; the Northeast Plant Index describes tobacco thistle growing under forests, by rivers, and in wet meadows. The Wild Vascular Plants of Northeast China Wetlands records that the habitat of tobacco thistle is: river floodplain. It can be seen that the three authoritative botanical monographs describe the habitat of tobacco thistle as mesophytic or xerophytic. This is consistent with the conclusion of Example 3, indicating that the result of determining that tobacco thistle belongs to a mesophytic plant (but not a typical wetland plant) by this method is reliable.

[0085] Example 4

[0086] Amaranthus retroflexus belongs to the genus Amaranth in the family Amaranthaceae. It is an annual herb with an erect, sturdy stem and densely pubescent. The leaves are rhombic-ovate, with entire or wavy margins, pubescent on both sides and margins, and denser on the underside. The panicles are terminal and axillary, erect, and formed by many spikes; the tepals are oblong, thin, and white; the stigmas are 3, sometimes 2. The utricles are flat-ovate. The seeds are nearly spherical, 1 mm in diameter, and brown or black.

[0087] Method for quantitatively determining typical wetland plants (analyzing whether they are typical wetland plants through the following steps):

[0088] 1. Target establishment: Take Amaranthus retroflexus distributed in the Sanjiang Plain as the target species to detect whether it is a typical wetland plant.

[0089] 2. Sample collection: In mid-to-late August, mature seeds of Amaranthus retroflexus were collected from farmlands, grasslands and other habitats in the Sanjiang Plain. Impurities were removed after air-drying in a cool place. After inspection, some seeds were found to be dormant. The top 20 cm of soil was dug from the seed collection site, air-dried, and roots, stones and other debris were removed. The seeds were ground and sieved through a 2 mm sieve for later use.

[0090] 3. Seed treatment: After the seeds treated in step 2 are disinfected with 75% alcohol, they are stored at low temperature and wet for 30 days to promote seed germination.

[0091] 4. Seed sowing: Evenly spread 50 seeds treated in step 3 in a plastic cup filled with soil obtained in step 2 (the plastic cup has a diameter of 10 cm, a height of 20 cm, and a small hole of 1 mm in diameter at the bottom to allow water to enter). The surface of the substrate should be flat and 5 cm away from the upper edge of the cup. Then cover the seeds with 0.3 cm of soil substrate.

[0092] 5. Water management: quickly place the culture cup in step 4 in a large culture tray, add appropriate amount of water, and achieve flooding (the flooding means the water level is 2 cm above the surface of the soil matrix) and moistening treatment (the moistening treatment means keeping the soil matrix 4 cm below the surface moist). Replenish water every day and record the emergence of seedlings. After 30 days, randomly select 5 plants to measure the average plant height (all plants less than 5 are measured).

[0093] 6. Type determination: Determine the plant type based on the seed germination rate and average plant height under the two water treatments.

[0094] Depend on Figure 1 It can be seen that the germination rate of Amaranthus retroflexus seeds in the wet treatment is as high as 70%, while the germination rate under 2 cm flooding is only 8% (11% in the wet treatment, less than 75%). Figure 2 It can be seen that the average plant height of Amaranthus retroflexus seeds was 17 cm 30 days after germination in the wet treatment, and the average plant height was only 3 cm under 2 cm flooding, which was 18% of the wet treatment. It can be seen that the germination rate and plant height of Amaranthus retroflexus seeds under 2 cm flooding treatment were much lower than the values ​​under wet treatment, meeting condition 3 (if the plant germination rate under flooding conditions is less than 75% of that under wet conditions, and the plant height is significantly lower than the value under wet conditions, it is a xerophyte and not a typical wetland plant). Therefore, Amaranthus retroflexus was determined to be a xerophyte.

[0095] Verification Example 4

[0096] According to the Flora of China, Amaranthus retroflexus grows in fields, beside farmland, and on grass near people's homes; the Northeast Plant Index describes Amaranthus retroflexus growing in fields, beside land, and beside houses. The Wild Vascular Plants of Northeast China Wetlands does not include it. It can be seen that the three authoritative botanical monographs describe the habitat of Amaranthus retroflexus as xerophytic in general. This is consistent with the conclusion of Example 4, indicating that the result of determining that Amaranthus retroflexus belongs to a xerophytic plant (not a typical wetland plant) by this method is reliable.

[0097] Summarize

[0098] It can be seen from the four embodiments that by comparing the emergence rate and seedling growth conditions of plant seeds under moistening and flooding treatments, and using the method established by the present invention, it can be determined whether Alisma orientalis, Cyperus capitatus, Cirsium truncatum and Amaranthus retroflexus are typical wetland plants. The conclusions obtained are consistent with the descriptions in authoritative monographs, which verifies the effectiveness of this method. This method follows the objective law that "the seedling stage is a critical stage in the life history of plants and is most sensitive to environmental changes". On the basis of conducting a large number of plant seedling renewal studies, two indicators are screened out to quantitatively determine whether a species is a typical wetland plant. The method is simple and scientific. In addition, the genotype of the same species may be different in different regions, and the ability to adapt to water conditions may also be different. This method also takes regional differences into account.

[0099] Considering that the seed germination experiment is simple to operate, has low requirements for equipment, and can detect multiple species at the same time, this method can be used to well determine whether a plant is a typical wetland plant, providing technical support for related work in the field of wetland science.

Claims

1. A method for quantitatively determining typical wetland plants, characterized in that The method for quantitatively determining typical wetland plants is as follows:

1. Target establishment: Determine the distribution area and suspected plant species according to the research objectives; 2. Sample collection: Collect seeds of target plants in the distribution area, air-dry them in a cool place, remove impurities, and check whether the seeds are dormant; dig 20 cm of soil from the surface of the seed collection site, air-dry it, remove roots and stones, grind it, and pass it through a 2 mm sieve for later use; 3. Seed treatment: After the seeds treated in step 2 are sterilized, they are stored in a low temperature and wet place for 30-120 days to promote seed germination; 4. Seed sowing: Evenly spread 10-80 seeds processed in step 2 or step 3 in a plastic cup containing the soil matrix obtained in step 2, requiring the surface of the soil matrix to be flat and 5 cm from the upper edge of the cup, and then cover the seeds with 0.2-0.5 cm of soil matrix; 5. Water management: quickly place the culture cup in step 4 in a large culture tray, add water to achieve flooding and moistening treatment; add water every day, and record the emergence of seedlings. After 10-30 days, randomly select 5 plants to measure the average plant height, and measure all plants with less than 5 plants; The flooding treatment means that the water level is 2 cm above the soil substrate surface, and the moistening treatment means that the soil substrate is kept moist 4 cm below the soil substrate surface; 6. Type determination: Determine the plant type based on the seed germination rate and average plant height under the two water treatments in step 5; The determination is specifically: If the plant emergence rate under flooded conditions is greater than or equal to 75% of that under wet conditions, and the plant height is greater than or equal to the value under wet conditions, it is a typical wetland plant; If the plant emergence rate under flooded conditions is greater than or equal to 75% of that under wet conditions, but the plant height is significantly less than the value under wet conditions, it is a mesophyte; mesophytes may occasionally appear in wetlands, but they are not typical wetland plants; If the plant emergence rate under flooded conditions is less than 75% of that under wet conditions, and the plant height is significantly lower than that under wet conditions, it is a xerophyte and is not a typical wetland plant.

2. The method for quantitatively determining typical wetland plants according to claim 1, characterized in that The area described in step 1 is an administrative level or a geographical division. The administrative levels include provinces and cities, and the geographical divisions include mountainous areas, plains, and plateaus.

3. The method for quantitatively determining typical wetland plants according to claim 1, characterized in that The lifespan of the seeds described in step 2 should be at least more than 1 month.

4. The method for quantitatively determining typical wetland plants according to claim 1, characterized in that The low temperature and wet storage described in step three is only for dormant seeds.

5. The method for quantitatively determining typical wetland plants according to claim 1, characterized in that The plastic cup described in step 4 has a diameter of 10 cm and a height of 20 cm, and has a small hole with a diameter of 1 mm at the bottom to allow water to enter.

6. The method for quantitatively determining typical wetland plants according to claim 1, characterized in that The number of seeds in step 4 is determined according to the size of the seeds; the smaller the seeds and the greater the number, the less soil matrix is ​​required to cover them.

7. The method for quantitatively determining typical wetland plants according to claim 1, characterized in that The duration of the experiment described in step 5 is determined according to the emergence of new seedlings. If no new seedlings emerge for 7 consecutive days, the experiment is considered to be over.

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