Bactericidal composition, pesticide containing bactericidal composition and application of bactericidal composition

Abstract

The invention discloses a bactericidal composition, a pesticide containing the bactericidal composition and application of the bactericidal composition, the bactericidal composition comprises albendazole and pydiflumetofen, and the mass ratio of the albendazole to the pydiflumetofen is (50: 1)-(1: 50). When the bactericidal composition is used for preventing and treating plant diseases, the dosage can be reduced, the bactericidal effect is good, meanwhile, the bactericidal spectrum is expanded, and the drug resistance of the diseases is delayed.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural fungicides, and more particularly to a fungicide composition containing propidazole and fluopicolide, a pesticide containing the fungicide composition and application thereof. Background Art

[0002] Propylthiocyanate, also known as propylthiocarbendazole, propylthiocarbendazole, and albendazole, belongs to the benzimidazole class of compounds. Its structure has one more propylthio group than carbendazim, and it is a low-toxic, broad-spectrum fungicide. Its mechanism of action is related to inhibiting the cell division of pathogens. It inhibits the polymerization of microtubules in fungal cells and the polymerization of bacterial fission proteins in bacteria. It has a good control effect on various diseases of various crops such as rice, wheat, and cucumbers, and can also promote the greening of crops and increase production and income.

[0003] Pydiflumetofen is a new fungicide developed by Syngenta of Switzerland. It belongs to the class of succinate dehydrogenase inhibitors (SDHI). This fungicide is a respiratory inhibitor of pathogens. It interferes with respiration, prevents them from producing energy, inhibits the growth of pathogens, and eventually kills the pathogens. Because of its novel structure, high activity, broad-spectrum fungi, and long-lasting effect, it is suitable for the prevention and control of fungal diseases on vegetables, corn, soybeans, grains, oil crops, rice, sugarcane and other crops, such as powdery mildew, leaf spot, rust, etc.

[0004] Since SDHI fungicides have a single action site, pathogens have a high risk of resistance to these agents, which will lead to higher and higher pesticide application rates and cause environmental pollution. Therefore, how to provide a fungicide to overcome the above-mentioned defects is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0005] In view of this, the present invention mixes propithiozole with fluopicolide, which can well overcome the drug resistance of the disease, improve the drug efficacy, reduce the amount of pesticide application, reduce economic losses, and protect the environment.

[0006] To achieve the above object, the present invention adopts the following technical solution:

[0007] Firstly, the present invention provides a fungicide composition, comprising: propiodazole and fluopicolide, wherein the mass ratio of propiodazole to fluopicolide is 50:1-1:50.

[0008] Preferably, the mass ratio of propithiozole to fluopicolide is 1:50, 1:20, 1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1, 20:1 or 50:1.

[0009] Preferably, the mass ratio of propithiozole to fluopicolide is 1:10-20:1.

[0010] Preferably, the mass ratio of propithiozole to fluopicolide is 1:5-5:1.

[0011] The present invention also provides a pesticide preparation containing the bactericidal composition, wherein the pesticide preparation comprises the bactericidal composition and a pesticide adjuvant, and the total mass of the bactericidal composition accounts for 1% to 90% of the composition.

[0012] Preferably, the pesticide adjuvant is one or more of a filler, a carrier, a solvent, an emulsifier, a wetting agent, a dispersant, a penetrant, a sticker, a stabilizer, a synergist, a defoamer, an antifreeze agent, a preservative, and a warning color.

[0013] Among them, the solvent is phenethylphenol polyoxyethylene ether phosphate, the emulsifier is xanthan gum, the wetting agent is polyethylene glycol, the dispersant is TERSPERSE2500 or sodium methylene bisnaphthalene sulfonate, the penetrant is polydimethylsiloxane emulsion, the adhesive is polyethylene glycol, the stabilizer is xanthan gum, the synergist is sodium benzoate, the defoaming agent is DF-830 / 831 / 832, the antifreeze is polydimethylsiloxane emulsion, and the preservative is sodium benzoate.

[0014] Preferably, the pesticide formulation is a suspension, which includes, by mass percentage, 10%-60% (preferably 10%-50%) of a fungicide composition, 0.1%-30% of a wetting agent, 0.1%-30% of a dispersant, 0.3%-30% of a penetrant, 0.5%-10% of a stabilizer, 1%-10% of an antifreeze agent, 1%-10% of a defoaming agent, 0.5%-3% of a preservative, and deionized water to make up to 100%.

[0015] The present invention also provides application of the bactericidal composition or the pesticide preparation containing the bactericidal composition in preventing and controlling plant diseases.

[0016] Preferably, the plant is a vegetable or a food crop.

[0017] Preferably, the disease is powdery mildew or rice blast.

[0018] It can be seen from the above technical scheme that, compared with the prior art, the present invention discloses a fungicide composition containing propidazole and fluopicolide, a pesticide containing the fungicide composition and its application, which have the following beneficial effects:

[0019] 1) Compared with single agent, compound agent has better synergistic effect and improves the control effect;

[0020] 2) The bactericidal composition of the present invention is composed of two active ingredients with different action mechanisms, which is beneficial for overcoming and delaying the development of drug resistance of pathogens;

[0021] 3) Mixing of drugs reduces the amount of drugs used, thereby reducing costs and reducing pollution to the environment. DETAILED DESCRIPTION

[0022] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example 1

[0024] 40% Propylene glycol·Flutriazole suspension (1:1)

[0025] Calculated by weight percentage, 20% of propiconazole, 20% of fluopicolide, 10% of phenethylphenol polyoxyethylene ether phosphate, 5% of TERSPERSE 2500, 5% of sodium methylene bisnaphthalene sulfonate, 3% of polyethylene glycol, 1% of xanthan gum, 0.1% of polydimethylsiloxane emulsion, 0.3% of sodium benzoate, and deionized water to make up 100%.

[0026] The above components are dispersed by high-speed shearing and sand-milled in a sand mill to form a highly dispersed and stable suspension system in an aqueous medium, thereby preparing a 40% propanthiocarb-flutrifloxystrobin suspension concentrate.

[0027] Example 2

[0028] 30% Propylene glycol·Flutriazole suspension (2:1)

[0029] By weight percentage, 20% of aprothioazole, 10% of fluopicolide, 8% of phenethylphenol polyoxyethylene ether phosphate, 5% of TERSPERSE2500, 5% of sodium methylenebisnaphthalene sulfonate, 4% of polyethylene glycol, 1% of xanthan gum, 0.1% of polydimethylsiloxane emulsion, 0.3% of sodium benzoate, and deionized water are added to 100%. The above ingredients are dispersed by high-speed shearing and sand-milled in a sand mill to form a highly dispersed and stable suspension system in an aqueous medium, thereby preparing a 30% aprothioazole fluopicolide suspension.

[0030] Example 3

[0031] 30% Propylene glycol·Flutriazole suspension (1:2)

[0032] By weight percentage, 10% of aprothioazole, 20% of fluopicolide, 7% of phenethylphenol polyoxyethylene ether phosphate, 5% of TERSPERSE2500, 4% of sodium methylenebisnaphthalene sulfonate, 5% of polyethylene glycol, 1% of xanthan gum, 0.1% of polydimethylsiloxane emulsion, 0.3% of sodium benzoate, and deionized water are added to 100%. The above ingredients are dispersed by high-speed shearing and sand-milled in a sand mill to form a highly dispersed and stable suspension system in an aqueous medium, thereby preparing a 30% aprothioazole fluopicolide suspension.

[0033] Test Example 1

[0034] Indoor joint toxicity of a mixture of propiconazole and pentoquinoline against rice blast

[0035] Test basis: NY / T1156.2-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth", NY / T1156.6-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 6: Determination of Combined Action of Mixtures".

[0036] Test materials: The test pathogen is rice blast fungus (Pyricularia oryzae Cav.).

[0037] Test agents: 98% propiconazole technical, Guizhou Daoyuan Biotechnology Co., Ltd.; 98% fluopicolide technical, Syngenta Nantong Crop Protection Co., Ltd.

[0038] Preparation of medicine: dissolve the original medicine in DMF, dilute it with 0.1% Tween 80 aqueous solution, and set 5 series of mass concentrations according to the activity of the medicine.

[0039] Preparation of culture medium: PDA culture medium is quantitatively dispensed into glass bottles before solidification and sterilized at high temperature. Under aseptic operation conditions, use a micropipette to inject 1 mL of the corresponding mother solution to be tested into each bottle of 49 mL of quantitative sterilized PDA culture medium (temperature 50-55°C) heated and melted, from low concentration to high concentration, shake well, and dispense into 4 culture dishes with a diameter of 9 cm to make drug-containing plates of corresponding concentrations. Prepare the culture medium for blank control plates.

[0040] Test method: Under aseptic conditions, use a sterilized puncher with an inner diameter of 0.5 cm to cut a bacterial cake from the edge of the colony. Move the bacterial cake to the center of the drug-containing culture medium, with the mycelium side facing down (the mycelium side is in contact with the culture medium), place a bacterial cake in each culture dish, place the inoculated culture dish in an SPJ-250B biochemical incubator, and culture at 28°C for 9 days, measure the colony diameter, calculate the percentage of mycelium growth inhibition by each drug treatment, and calculate EC50 through linear regression analysis between the probability value of the inhibition rate and the logarithm of the series concentration.

[0041] The interaction of mixed drugs was calculated according to Sun Yunpei's method: CTC≤80 was antagonistic, CTC≥120 was synergistic, and CTC between 80 and 120 was additive.

[0042] Calculation method:

[0043] Mycelial growth inhibition rate (%) = [(blank control colony growth diameter - agent-treated colony growth diameter) / blank control colony growth diameter] × 100 The measured toxicity index (ATI) of the mixture = (EC50 of the standard agent / EC50 of the mixture) × 100

[0044] Theoretical toxicity index (TTI) of the mixture = toxicity index of agent A × percentage of agent A in the mixture + toxicity index of agent B × percentage of agent B in the mixture

[0045] Co-toxicity coefficient (CTC) = [Actual toxicity index (ATI) of the mixture / Theoretical toxicity index (TTI) of the mixture] × 100

[0046] Test results:

[0047] Table 1 Indoor joint toxicity of albendazole and fluopicolide mixture to rice blast

[0048]

[0049]

[0050] As shown in Table 1, when propidazole and fluopicolide are compounded in the range of 1:10-20:1 (by weight), the co-toxicity coefficient (CTC) is greater than 120, indicating that they have a synergistic effect on rice blast fungus. It can be seen that the compounding of propidazole and fluopicolide is reasonable and feasible. The synergy of propidazole and fluopicolide is most obvious at a ratio of 2:1, and the maximum co-toxicity coefficient is 160.2350.

[0051] Test Example 2

[0052] Field efficacy of a mixture of propithiozole and pentocyclamide against rice blast

[0053] Test basis: In accordance with the national standard "Guidelines for Field Efficacy Tests of Pesticides (I)": Fungicides for the prevention and control of rice leaf diseases (GB / T17980.19-2000).

[0054] Test object: Rice blast.

[0055] Experimental crops: Rice 'Yongyou 9'.

[0056] Test location: Rice fields in Jiaoxi Village, Shunchang County, Nanping City, Fujian Province. The soil fertility is above average, and the row and plant spacing is 20×20cm.

[0057] Experimental agents: 40% albithioazole·flutriazole suspension (1:1), 30% albithioazole·flutriazole suspension (2:1), 30% albithioazole·flutriazole suspension (1:2), 20% albithioazole suspension, 200 g / L flutriazole suspension, and a clean water control was set up.

[0058] Test method: According to the set test agent dosage table (see Table 2), spray evenly at the early stage of rice blast. Each treatment was repeated 4 times, each plot was 40 square meters, and the plots were arranged in random blocks. During the survey, five points were sampled in each plot, and 50 ears were surveyed at each point, for a total of 250 ears.

[0059] Investigation method: Conduct an efficacy investigation 10 days after application of the drug, record the total number of panicles and the number of diseased panicles at each level, and the classification standards for rice panicle blast are as follows:

[0060] Level 0: no disease;

[0061] Level 1: less than 5% loss per ear (individual stalks are diseased);

[0062] Level 3: 6%-20% loss per ear (about one-third of the branches and stalks are diseased);

[0063] Level 5: 21%-50% loss per ear (the neck or main axis is diseased, and the grains are half-shrunken);

[0064] Level 7: 51%-70% loss per ear (ear neck disease, most of the grains are shrunken);

[0065] Level 9: 71%-100% loss per ear (the ear neck becomes diseased, resulting in white ears).

[0066] Calculation formula:

[0067] Disease index = Σ(number of diseased ears at each level × relative level value) / (total number of ears surveyed × 9) × 100

[0068] Control effect (%) = (disease index of blank control area - disease index of treated area) / disease index of blank control area × 100

[0069] The test results are shown in Table 2:

[0070] Table 2 Control effect of albendazole and fluopicolide on rice blast 10 days after application

[0071]

[0072]

[0073] Field efficacy showed (see Table 2) that the mixture of albendazole and fluopicolide showed significantly better antibacterial activity against rice blast than a single agent. Ten days after application, the control effects of 40% albendazole·fluopicolide suspension (1:1), 30% albendazole·fluopicolide suspension (2:1), and 30% albendazole·fluopicolide suspension (1:2) were all greater than 75%, among which 30% albendazole·fluopicolide suspension (2:1) had the best control effect of 82.39%.

[0074] Test Example 3

[0075] Field efficacy of a mixture of propithiozole and pentocyclamide against cucumber powdery mildew

[0076] Test basis: According to the national standard "Guidelines for Field Efficacy Tests of Pesticides (I)" Part 30: Fungicides for Control of Cucumber Powdery Mildew (GB / T17980.30-2000).

[0077] Test object: cucumber powdery mildew.

[0078] Experimental crop: Cucumber 'Toyota 16'.

[0079] Test location: Vegetable greenhouse at Yuxi Army Farm, Fuqing City, Fujian Province, with a row and plant spacing of 60×100cm and good growth.

[0080] Experimental agents: 40% albithioazole·flutriazole suspension (1:1), 30% albithioazole·flutriazole suspension (2:1), 30% albithioazole·flutriazole suspension (1:2), 20% albithioazole suspension, 200 g / L flutriazole suspension, and a clean water control was set up.

[0081] Test method: The test was set up according to the set test drug dosage (see Table 3). The test drug, control drug and blank control plots were arranged in random blocks. Each plot was 30 square meters in area, and each group was treated 4 times. The drug was sprayed twice, with an interval of about 10 days. The control effect was investigated one day after the drug was sprayed. Four points were randomly selected in each plot, and all leaves of two plants were investigated at each point. Each leaf was graded and recorded according to the percentage of the lesion to the leaf area.

[0082] Disease classification:

[0083] Level 0: no lesions;

[0084] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0085] Level 3: The lesion area accounts for 6%-10% of the entire leaf area;

[0086] Level 5: The lesion area accounts for 11%-20% of the entire leaf area;

[0087] Level 7: The lesion area accounts for 21%-40% of the entire leaf area;

[0088] Level 9: The lesion area accounts for more than 40% of the total leaf area.

[0089] Calculation formula:

[0090] Disease index = Σ(number of diseased leaves at each level × relative level value) / (total number of leaves investigated × 9) × 100

[0091] Control effect (%) = (disease index of blank control area - disease index of treated area) / disease index of blank control area × 100

[0092] The test results are shown in Table 3:

[0093] Table 3 Control effect of propanthazole and fluopicolide mixed on cucumber powdery mildew 12 days after application

[0094]

[0095]

[0096] Field efficacy showed (see Table 3) that the mixture of albendazole and fluopicolide showed significantly better antibacterial activity against cucumber powdery mildew than a single agent. Twelve days after application, the control effects of 40% albendazole·fluopicolide suspension (1:1), 30% albendazole·fluopicolide suspension (2:1), and 30% albendazole·fluopicolide suspension (1:2) were all greater than 80%, among which 30% albendazole·fluopicolide suspension (2:1) had the best control effect of 87.30%.

[0097] In summary, through indoor toxicity determination and field efficacy test, the fungicidal composition containing propidazole and fluopicolide described in the present invention has a good control effect on rice blast and cucumber powdery mildew, and is safe for target crops, has significant control effect, can effectively reduce costs, and reduce drug residues.

[0098] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0099] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A bactericidal composition, characterized in that: include: Propionazole and fluopicolide, wherein the mass ratio of propionazole to fluopicolide is 50:1-1:

50.

2. A bactericidal composition according to claim 1, characterized in that: The mass ratio of propithiazole to fluopicolide is 1:50, 1:20, 1:10, 1:5, 1:2, 1:1, 2:1, 5:1, 10:1, 20:1 or 50:

1.

3. A bactericidal composition according to claim 1, characterized in that: The mass ratio of propithiozole to fluopicolide is 1:10-20:

1.

4. A bactericidal composition according to claim 1, characterized in that: The mass ratio of propithiozole to fluopicolide is 1:5-5:

1.

5. A pesticide formulation comprising the fungicidal composition according to any one of claims 1 to 4, characterized in that: The invention comprises the bactericidal composition and the pesticide adjuvant, and the mass of the bactericidal composition accounts for 1% to 90% of the composition.

6. The pesticide formulation according to claim 5, characterized in that: The pesticide adjuvant is one or more of a filler, a carrier, a solvent, an emulsifier, a wetting agent, a dispersant, a penetrant, a sticker, a stabilizer, a synergist, a defoamer, an antifreeze agent, a preservative, and a warning color.

7. The pesticide formulation according to claim 6, characterized in that: The pesticide preparation is a suspension, which comprises, by mass percentage, 10%-60% of a bactericidal composition, 0.1%-30% of a wetting agent, 0.1%-30% of a dispersant, 0.3%-30% of a penetrant, 0.5%-10% of a stabilizer, 1%-10% of an antifreeze agent, 1%-10% of a defoaming agent, 0.5%-3% of a preservative, and deionized water to make up 100%.

8. Use of the fungicide composition according to any one of claims 1 to 4 or the pesticide preparation containing the fungicide composition according to any one of claims 5 to 7 in preventing and controlling plant diseases.

9. The use according to claim 8, characterized in that: The plants are vegetables or food crops.

10. The use according to claim 8, characterized in that: The disease is powdery mildew or rice blast.

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