Production method of pig, cattle and chicken mixed bone collagen peptide and collagen peptide
Through the production method of pig, cattle and chicken mixed bone collagen peptides, combined with the advantages of multiple bone sources, high-pressure steam oil removal, cooking, vacuum concentration and enzymatic decomposition are used to solve the problem of insufficient resource utilization in a single bone source technology. , improves the purity of collagen peptides and the absorption rate of the human body, and meets the human body's demand for a variety of nutrients.
Patent Information
- Application Number
- CN202411609785.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-01-10
AI Technical Summary
The existing collagen peptide production technology mainly relies on a single bone source, and cannot fully utilize the complementary advantages of nutrients between different bone sources, resulting in a single nutritional component of the product, insufficient resource utilization, low economic benefits, and unable to meet the human body's multiple nutritional components. compound needs.
The production method of pig, cow and chicken mixed bone collagen peptide is adopted. Collagen is extracted by mixing pig bones, cow bones and chicken bone residues in a specific proportion, combined with high-pressure steam oil removal, cooking, vacuum concentration, enzymatic decomposition and spray drying. Protein-chondroitin sulfate mixture is used for enzymatic decomposition using complex protease, and filtered through activated carbon and filter membrane to improve the purity and utilization of collagen peptides.
It realizes the efficient utilization of a variety of bone source resources, generates high-purity and easy-to-absorb collagen peptides, improves the utilization rate of animal aggregates, meets the human body's needs for a variety of nutrients, and promotes bone health.
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Figure BDA0005130641070000071
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of collagen peptide production, and in particular to a production method of pig, cattle and chicken mixed bone collagen peptide and collagen peptide. Background Art
[0002] Collagen peptides are important components for joint and bone health. Human articular cartilage is built by chondrocytes. These chondrocytes produce an extracellular matrix of collagen and proteoglycans. Collagen is responsible for the structure and strength of articular cartilage, while proteoglycans lubricate and cushion the joints. Healthy articular cartilage relies on a perfectly balanced process of collagen and proteoglycan breakdown and formation.
[0003] At present, the technology of extracting nutrients such as collagen peptides and chondroitin sulfate from animal bones has been widely studied. However, most methods only extract from a single bone source and fail to fully utilize the complementary advantages of nutrients between different bone sources. The nutritional components of products extracted from a single bone source are relatively single and cannot meet the complex needs of the human body for multiple nutrients. At the same time, resource utilization is not sufficient and the economic benefits are low. Summary of the invention
[0004] In order to improve the impact of the single bone source of traditional collagen peptides, the present application provides a production method of pig, beef and chicken mixed bone collagen peptides and collagen peptides.
[0005] In the first aspect, the present application provides a method for producing mixed collagen peptides of pig, cattle and chicken, using the following technical solution: A method for producing mixed pig, cattle and chicken collagen peptides comprises the following specific steps: Mixing pig bones, cow bones and chicken bone residues to form a mixed bone source, wherein the mass ratio of the pig bones, cow bones and chicken bone residues is 4:(2.5-3.5):(2.5-3.5), then removing bone oil in the mixed bone source by high-pressure steam, and then extracting a collagen-chondroitin sulfate mixed solution by steaming; The collagen-chondroitin sulfate mixture is vacuum concentrated until the solid content of the collagen-chondroitin sulfate mixture reaches 20-30Rrix, and then a composite protease is added to the collagen-chondroitin sulfate mixture for enzymatic hydrolysis to form an enzymatic hydrolysis product, and the enzymatic hydrolysis product is spray-dried to obtain pig, cattle and chicken mixed bone collagen peptides.
[0006] By adopting the above technical scheme, pig bones and cattle bones have a higher collagen content, and chicken bones have a high chondroitin sulfate content. Different bone raw materials of pigs, cattle and chickens are reasonably matched, and the co-production process is used to separate and extract collagen peptides and chondroitin sulfate and other mucopolysaccharide composite products to meet the human nutrient intake needs and improve the utilization value of pig, cattle and chicken bone resources. By reasonably matching pigs, cattle and chickens, and optimizing the combination of high-pressure steam degreasing, cooking, vacuum concentration, enzymatic hydrolysis and spray drying, a large amount of high-quality collagen peptides are obtained, which improves the utilization rate of animal bone materials.
[0007] Preferably, the mass content of collagen peptides with a molecular weight of ≤5000 kDa in the mixed bone collagen peptides of pig, cattle and chicken is ≥80%, and the mass content of chondroitin sulfate is ≥10%.
[0008] By adopting the above technical scheme, the prepared collagen peptides have a higher content of small molecule collagen peptides, which can achieve full degradation of bone collagen and generate protein peptide products with smaller molecular weight, which are easier to be absorbed and utilized by the human body.
[0009] Preferably, the composite protease comprises at least two of papain, trypsin and alkaline protease, and the usage amount of the composite protease is 0.5-0.8% of the mass of the collagen-chondroitin sulfate mixed solution.
[0010] By adopting the above technical solution, using papain, trypsin and alkaline protein for enzymatic hydrolysis, it is possible to hydrolyze collagen in a targeted manner, decompose the macromolecular collagen in the mixed aggregate into small molecular peptide segments, and generate collagen peptides. The generated collagen peptides can be effectively absorbed by the human body, improve human bone density and bone toughness, and promote human bone health. Using papain, trypsin and alkaline protein as a composite protease, chondroitin sulfate can be accurately released in mixed bone-derived tissues, and proteins connected to chondroitin sulfate can be enzymatically hydrolyzed, thereby ensuring the structural integrity of chondroitin sulfate. The composite protease promotes the separation and purification of collagen peptides and chondroitin sulfate, and improves the extraction and biological activity of collagen peptides.
[0011] Preferably, the enzymolysis temperature is 40-50°C and the enzymolysis time is 3-4h.
[0012] Preferably, the enzymatic hydrolysis product is filtered using activated carbon to obtain a primary filtrate, and then the primary filtrate is ultrafiltered using a filter membrane, and the filtrate is spray-dried to obtain a mixed pig, cattle and chicken collagen peptide.
[0013] By adopting the above technical solution, activated carbon is used to adsorb and filter the enzymatic hydrolysis products. The powerful adsorption capacity of activated carbon can be used to effectively remove small molecular impurities, pigments and odorous substances generated during the enzymatic hydrolysis process, play a role in deodorization, adsorption and filtration, and improve the purity and quality of collagen peptides.
[0014] Preferably, the filter membrane is divided into a primary filter membrane and a secondary filter membrane, the molecular weight cutoff of the primary filter membrane is 8-10 kDa, and the molecular weight cutoff of the secondary filter membrane is 3-5 kDa.
[0015] By adopting the above technical solution, the enzymatic hydrolysis product is ultrafiltered through filter membranes of different sizes, and small molecular impurities such as residual enzymes and small peptide fragments are removed more accurately to improve the purity of the product. By utilizing the difference in the size of the two-stage filter membrane, the content of small molecular weight protein peptides in the collagen peptide product is increased, and the target protein peptide is concentrated, which can promote the molecular weight of the prepared collagen peptide to have better uniformity. At the same time, the two-stage filter membrane can also serve as a physical barrier to reduce microbial contamination and improve the purity and quality of collagen peptides.
[0016] Preferably, the mixed bone source is immersed in an activation solution in advance for activation treatment, and then washed to obtain the activated mixed bone source, and then high-pressure steam is used to remove the bone oil in the activated mixed bone source.
[0017] By adopting the above technical solution and activating the mixed bone source in advance, the subsequent enzymatic hydrolysis time and enzyme dosage can be reduced, the interference of minerals in animal bones in the extraction of collagen peptides can be reduced, the generation of by-products can be reduced, and the extraction amount and purity of collagen peptides can be improved.
[0018] Preferably, the activation solution is a mixture of lactic acid and citric acid, and the mass ratio of the lactic acid to the citric acid is 1:(0.5-0.8).
[0019] By adopting the above technical solution, emulsification and citric acid can make the activation liquid present an acidic ring, which is conducive to the softening of animal bones, making the collagen structure easier to enzymatically hydrolyze, promoting the dissolution of collagen fibers, enhancing the contact efficiency between the compound protease and collagen, and improving the enzymatic efficiency. Lactic acid and citric acid can dissolve the minerals in animal bones, reduce the interference of calcium, phosphorus and other substances on collagen extraction, and can also activate the activity of the compound protease, accelerate the degradation rate of collagen, and promote the generation of collagen peptides.
[0020] In the second aspect, the present application provides a mixed collagen peptide of pig, cattle and chicken, which adopts the following technical solution: A mixed collagen peptide of pig, cattle and chicken is prepared by the production method.
[0021] By adopting the above technical solution, the prepared collagen peptide has higher purity, solubility and quality, and can be more easily absorbed by the human body.
[0022] In summary, this application has the following beneficial effects: 1. Since this application uses a reasonable combination of mixed bone sources of pigs, cattle and chickens, collagen peptides and chondroitin sulfate and other mucopolysaccharide composite products are separated and extracted through high-pressure steam degreasing, cooking, vacuum concentration, enzymatic hydrolysis and spray drying, thereby improving the utilization rate of animal aggregates and obtaining a large amount of high-quality collagen peptides.
[0023] 2. In this application, activated carbon is used to filter the enzymatic hydrolysis products, which can adsorb and remove impurities, remove odors, and improve product quality and purity. Then, the enzymatic hydrolysis products are filtered using two-stage filtration membranes of different sizes to more accurately remove small molecular impurities such as residual enzymes and small peptides, concentrate target protein peptides, and improve product purity. DETAILED DESCRIPTION
[0024] The present application is further described in detail below with reference to the embodiments.
[0025] All raw materials in the examples are commercially available. Example
[0026] Example 1 This embodiment provides a method for producing mixed pig, cattle and chicken collagen peptides, comprising the following specific steps: S1: Mix pig bones, cow bones and chicken bone residues to form a mixed bone source. The mass ratio of pig bones, cow bones and chicken bone residues is 4:3:3. Heat to 120°C with high-pressure steam at 12MPa to remove bone oil in the mixed bone source, and then steam for 20 minutes to extract the collagen-chondroitin sulfate mixture.
[0027] S2: The collagen-chondroitin sulfate mixture is vacuum concentrated until the solid content of the collagen-chondroitin sulfate mixture reaches 25Rrix, and then a composite protease is added to the collagen-chondroitin sulfate mixture for enzymatic hydrolysis. The amount of the composite protease used is 0.7% of the mass of the collagen-chondroitin sulfate mixture, wherein the composite protease is a mixture of papain and trypsin, and the mass ratio of papain and trypsin is 1:1. Enzymatic hydrolysis is carried out at 45°C for 3h to form an enzymatic hydrolysis product, and the enzymatic hydrolysis product is spray-dried to obtain pig, cattle and chicken mixed bone collagen peptides.
[0028] This embodiment provides a mixed collagen peptide of pig, cattle and chicken, which is prepared by the above production method.
[0029] Example 2 The difference between Example 2 and Example 1 is that in the production method of the mixed bone collagen peptide of pig, cattle and chicken, the mass ratio of pig bone, cattle bone and chicken bone residue is 4:2.5:3.5.
[0030] Example 3 The difference between Example 3 and Example 1 is that in the production method of the mixed bone collagen peptide of pig, cattle and chicken, the mass ratio of pig bone, cattle bone and chicken bone residue is 4:3.5:2.5.
[0031] Example 4 The difference between Example 4 and Example 1 is that in the production method of the mixed pig, cattle and chicken collagen peptide, the enzymatic hydrolysis product is filtered using activated carbon.
[0032] A method for producing mixed pig, cattle and chicken collagen peptides comprises the following specific steps: S1: Mix pig bones, cow bones and chicken bone residues to form a mixed bone source. The mass ratio of pig bones, cow bones and chicken bone residues is 4:3:3. Heat to 120°C with high-pressure steam at 12MPa to remove bone oil in the mixed bone source, and then steam for 20 minutes to extract the collagen-chondroitin sulfate mixture.
[0033] S2: The collagen-chondroitin sulfate mixture is vacuum concentrated until the solid content of the collagen-chondroitin sulfate mixture reaches 25Rrix, and then a composite protease is added to the collagen-chondroitin sulfate mixture for enzymatic hydrolysis. The amount of the composite protease used is 0.7% of the mass of the collagen-chondroitin sulfate mixture, wherein the composite protease is a mixture of papain and trypsin, and the mass ratio of papain to trypsin is 1:1. Enzymatic hydrolysis is carried out at 45°C for 3 hours to form an enzymatic hydrolysis product.
[0034] S3: The enzymatic hydrolysis product is adsorbed by activated carbon, the amount of activated carbon used is 2% of the volume of the enzymatic hydrolysis product, and after adsorption for 1.5 hours, the product is filtered and the filtrate is spray-dried to obtain the mixed collagen peptides of pig, cattle and chicken.
[0035] This embodiment provides a mixed collagen peptide of pig, cattle and chicken, which is prepared by the above production method.
[0036] Example 5 The difference between Example 5 and Example 4 is that in the production method of the mixed collagen peptide of pig, cattle and chicken, a filter membrane is used to ultrafilter the filtrate after the activated carbon adsorption.
[0037] A method for producing mixed pig, cattle and chicken collagen peptides comprises the following specific steps: S1: Mix pig bones, cow bones and chicken bone residues to form a mixed bone source. The mass ratio of pig bones, cow bones and chicken bone residues is 4:3:3. Heat to 120°C with high-pressure steam at 12MPa to remove bone oil in the mixed bone source, and then steam for 20 minutes to extract the collagen-chondroitin sulfate mixture.
[0038] S2: The collagen-chondroitin sulfate mixture is vacuum concentrated until the solid content of the collagen-chondroitin sulfate mixture reaches 25Rrix, and then a composite protease is added to the collagen-chondroitin sulfate mixture for enzymatic hydrolysis. The amount of the composite protease used is 0.7% of the mass of the collagen-chondroitin sulfate mixture, wherein the composite protease is a mixture of papain and trypsin, and the mass ratio of papain to trypsin is 1:1. Enzymatic hydrolysis is carried out at 45°C for 3 hours to form an enzymatic hydrolysis product.
[0039] S3: The enzymatic hydrolysis product is adsorbed by activated carbon, the amount of activated carbon used is 2% of the volume of the enzymatic hydrolysis product, and filtered after adsorption for 1.5 hours to obtain a primary filtrate, and then the primary filtrate is ultrafiltered twice through a filter membrane with a molecular weight of 9 kDa, the filtrate is collected, and the filtrate is spray-dried to obtain pig, cattle and chicken mixed collagen peptides.
[0040] This embodiment provides a mixed collagen peptide of pig, cattle and chicken, which is prepared by the above production method.
[0041] Example 6 The difference between Example 6 and Example 5 is that in the production method of the mixed collagen peptide of pig, cattle and chicken, a two-stage filtration membrane is used to ultrafilter the filtrate after activated carbon adsorption.
[0042] A method for producing mixed pig, cattle and chicken collagen peptides comprises the following specific steps: S1: Mix pig bones, cow bones and chicken bone residues to form a mixed bone source. The mass ratio of pig bones, cow bones and chicken bone residues is 4:3:3. Heat to 120°C with high-pressure steam at 12MPa to remove bone oil in the mixed bone source, and then steam for 20 minutes to extract the collagen-chondroitin sulfate mixture.
[0043] S2: The collagen-chondroitin sulfate mixture is vacuum concentrated until the solid content of the collagen-chondroitin sulfate mixture reaches 25Rrix, and then a composite protease is added to the collagen-chondroitin sulfate mixture for enzymatic hydrolysis. The amount of the composite protease used is 0.7% of the mass of the collagen-chondroitin sulfate mixture, wherein the composite protease is a mixture of papain and trypsin, and the mass ratio of papain to trypsin is 1:1. Enzymatic hydrolysis is carried out at 45°C for 3 hours to form an enzymatic hydrolysis product.
[0044] S3: The enzymatic hydrolysis product is adsorbed by activated carbon, the amount of activated carbon used is 2% of the volume of the enzymatic hydrolysis product, and filtered after adsorption for 1.5 hours to obtain a primary filtrate, and then the primary filtrate is successively passed through a filter membrane with a molecular weight of 9 kDa and a filter membrane with a molecular weight of 4 kDa for ultrafiltration, and ultrafiltration is performed twice respectively. The filtrate is collected and spray-dried to obtain a mixed pig, cattle and chicken collagen peptide.
[0045] This embodiment provides a mixed collagen peptide of pig, cattle and chicken, which is prepared by the above production method.
[0046] Example 7 The difference between Example 7 and Example 6 is that in the production method of the mixed bone collagen peptides of pig, cattle and chicken, the mixed bone source is activated in advance.
[0047] A method for producing mixed pig, cattle and chicken collagen peptides comprises the following specific steps: S1: Mix pig bones, cattle bones and chicken bone residues to form a mixed bone source, the mass ratio of pig bones, cattle bones and chicken bone residues is 4:3:3, soak the mixed bone source in an activation solution with an emulsified mass fraction of 8% for 1 hour, and the ratio of the mixed bone source to the activation solution is 1g:15mL, then take out the mixed bone source and wash it to neutrality to obtain an activated mixed bone source. Heat it to 120°C with high-pressure steam at 12MPa to remove the bone oil in the activated mixed bone source, and then extract the collagen-chondroitin sulfate mixed solution by boiling for 20 minutes.
[0048] S2: The collagen-chondroitin sulfate mixture is vacuum concentrated until the solid content of the collagen-chondroitin sulfate mixture reaches 25Rrix, and then a composite protease is added to the collagen-chondroitin sulfate mixture for enzymatic hydrolysis. The amount of the composite protease used is 0.7% of the mass of the collagen-chondroitin sulfate mixture, wherein the composite protease is a mixture of papain and trypsin, and the mass ratio of papain to trypsin is 1:1. Enzymatic hydrolysis is carried out at 45°C for 3 hours to form an enzymatic hydrolysis product.
[0049] S3: The enzymatic hydrolysis product is adsorbed by activated carbon, the amount of activated carbon used is 2% of the volume of the enzymatic hydrolysis product, and filtered after adsorption for 1.5 hours to obtain a primary filtrate, and then the primary filtrate is successively passed through a filter membrane with a molecular weight of 9 kDa and a filter membrane with a molecular weight of 4 kDa for ultrafiltration, and ultrafiltration is performed twice respectively. The filtrate is collected and spray-dried to obtain a mixed pig, cattle and chicken collagen peptide.
[0050] This embodiment provides a mixed collagen peptide of pig, cattle and chicken, which is prepared by the above production method.
[0051] Example 8 The difference between Example 8 and Example 7 is that in the production method of the mixed collagen peptides of pig, cattle and chicken, the activation liquid is a mixture of lactic acid and citric acid, and the mass ratio of lactic acid to citric acid is 1:0.6.
[0052] Comparative Example Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that a single pig bone source is used in the production method of the mixed pig, beef and chicken collagen peptide.
[0053] Performance Testing The following performance tests were conducted on the mixed pig, cattle and chicken collagen peptides provided in Examples 1-8 and Comparative Example 1 of the present application. The specific test results are shown in Table 1.
[0054] Detection Methods 1. Determination of chondroitin sulfate content With reference to the standard of GB / T20365-2006 "Determination of the Content of Chondroitin Sulfate and Glucosamine Hydrochloride", the chondroitin sulfate content in the mixed bone collagen peptides of pig, cattle and chicken produced in this application was quantitatively analyzed using the external standard method.
[0055] 2. Molecular weight distribution of mixed collagen peptides from pigs, cattle and chicken The molecular weight distribution of mixed bone collagen peptides from pigs, cattle and chickens was determined by high performance liquid gel chromatography, and the content of collagen peptides with a molecular weight of ≤5000 kDa was determined.
[0056] 3. Physical and chemical indicators of mixed collagen peptides from pigs, cattle and chickens The protein content in the mixed bone collagen peptides of pig, cattle and chicken produced in this application was detected by referring to the Lowry method, and the ash and fat content in the mixed bone collagen peptides of pig, cattle and chicken were detected by referring to the detection standard of GB31645-2018 "National Food Safety Standard Collagen Peptide".
[0057] Table 1: Performance test results data table It can be seen from the performance test results that the mixed bone collagen peptides of pig, cattle and chicken prepared in the present application are rich in collagen peptides and chondroitin sulfate, which meet the nutrient intake needs of the human body and improve the utilization rate of animal aggregates.
[0058] From the comparison between Example 4 and Example 1, it can be seen that in Example 4, the enzymatic hydrolysis product was filtered using activated carbon, and from the physical and chemical index detection of the mixed bone collagen peptides of pig, cattle and chicken, it can be seen that the impurity content in the collagen peptide product is significantly reduced, and the purity of the produced collagen peptide is increased.
[0059] From the comparison between Example 5-6 and Example 1, it can be seen that Example 5-6 uses a filter membrane to ultrafilter the filtrate after activated carbon adsorption, and a two-stage filter membrane is used in Example 6. From the test results, it can be seen that the purity of the collagen peptide product is significantly increased, the quality is improved, and the content of chondroitin sulfate and low molecular weight protein peptides is increased.
[0060] From the comparison between Example 7-8 and Example 1, it can be seen that Example 7-8 uses different activation solutions to pre-activate the mixed bone source. From the test results, it can be seen that the content of chondroitin sulfate and the content of small molecule protein peptides in the collagen peptide product are significantly increased, and the collagen peptide has a higher purity.
[0061] From the comparison between Comparative Example 1 and Example 1, it can be seen that Example 1 uses a single bone source, and the content of chondroitin sulfate and small molecule protein peptides in the produced collagen peptides are lower, and the impurity content in the collagen peptide product is also higher.
[0062] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed. However, as long as it is within the scope of the claims of the present application, it shall be protected by the patent law.
Claims
1. A method for producing mixed collagen peptides of pig, cattle and chicken, characterized in that: The specific steps include: Mixing pig bones, cow bones and chicken bone residues to form a mixed bone source, wherein the mass ratio of the pig bones, cow bones and chicken bone residues is 4: (2.5-3.5): (2.5-3.5), then removing bone oil in the mixed bone source by high-pressure steam, and then extracting collagen-chondroitin sulfate mixed solution by steaming; The collagen-chondroitin sulfate mixture is vacuum concentrated until the solid content of the collagen-chondroitin sulfate mixture reaches 20-30Rrix, and then a composite protease is added to the collagen-chondroitin sulfate mixture for enzymatic hydrolysis to form an enzymatic hydrolysis product, and the enzymatic hydrolysis product is spray-dried to obtain pig, cattle and chicken mixed bone collagen peptides.
2. The method for producing mixed pig, cattle and chicken collagen peptide according to claim 1, characterized in that: The mass content of collagen peptides with a molecular weight of ≤5000 kDa in the mixed bone collagen peptides of pig, cattle and chicken is ≥80%, and the mass content of chondroitin sulfate is ≥10%.
3. The method for producing mixed collagen peptides of pig, cattle and chicken according to claim 1, characterized in that: The composite protease comprises at least two of papain, trypsin and alkaline protease, and the usage amount of the composite protease is 0.5-0.8% of the mass of the collagen-chondroitin sulfate mixed solution.
4. The method for producing mixed collagen peptides of pig, cattle and chicken according to claim 3, characterized in that: The enzymolysis temperature is 40-50°C, and the enzymolysis time is 3-4h.
5. The method for producing mixed pig, cattle and chicken collagen peptide according to claim 1, characterized in that: The enzymatic hydrolysis product is filtered using activated carbon to obtain a primary filtrate, and the primary filtrate is then ultrafiltered using a filter membrane. The filtrate is spray-dried to obtain a mixed pig, cattle and chicken collagen peptide.
6. The method for producing mixed pig, cattle and chicken collagen peptide according to claim 5, characterized in that: The filter membrane is divided into a primary filter membrane and a secondary filter membrane. The molecular weight cutoff of the primary filter membrane is 8-10 kDa, and the molecular weight cutoff of the secondary filter membrane is 3-5 kDa.
7. The method for producing mixed collagen peptides of pig, cattle and chicken according to claim 1, characterized in that: The mixed bone source is immersed in an activation solution in advance for activation treatment, and then washed to obtain the activated mixed bone source. The bone oil in the activated mixed bone source is then removed using high-pressure steam.
8. The method for producing mixed collagen peptides of pig, cattle and chicken according to claim 7, characterized in that: The activation solution is a mixture of lactic acid and citric acid, and the mass ratio of the lactic acid to the citric acid is 1:(0.5-0.8).
9. A mixed collagen peptide of pig, cattle and chicken, characterized in that: The pig, cattle and chicken mixed collagen peptide is produced by the production method according to any one of claims 1-8.