Automatic plant tissue culture device

Abstract

The invention provides an automatic plant tissue culture device which comprises a rack, a station switching mechanism is arranged on the rack, the station switching mechanism comprises a first motor, and the end of an output shaft of the first motor extends to the top of the rack in the vertical direction and is fixedly provided with a disc; the disc is driven by a first motor to rotate and sequentially passes through an upper line bowl taking mechanism, a nutrient solution injection mechanism, a plant tissue conveying machine, a plant tissue shearing mechanism, an automatic packaging machine, an lower line bowl taking mechanism and a lower line conveying machine which are arranged on the rack. According to the invention, through the station switching mechanism, the on-line bowl taking mechanism, the nutrient solution injection mechanism, the plant tissue conveyor, the plant tissue shearing mechanism, the automatic packaging machine, the off-line bowl taking mechanism and the off-line conveyor, the whole process of automatic bowl taking, automatic conveying, automatic plant tissue shearing, automatic inoculation and automatic packaging off-line is automatically operated; the labor intensity of manual tissue culture is reduced, and the tissue culture efficiency is effectively improved.

Description

Technical Field

[0001] The invention mainly relates to the technical field of plant tissue culture, and in particular to an automated plant tissue culture device. Background Art

[0002] Plant tissue culture is the process of inoculating plant tissue into a culture medium in a tissue culture pot under sterile conditions to cultivate it into a complete plant. Generally, a large number of tissue culture pots are used for plant tissue culture, and a lot of labor is required for cutting and inoculating plant tissues. The probability of failure of artificial plant tissue culture due to contamination is also high.

[0003] Publication No.: CN213214723U, discloses a Dendrobium officinale tissue culture filling device, including a base frame, the inner front wall and the inner rear wall of the base frame are rotatably connected to a rotating shaft through bearings, the number of the rotating shafts is two, the surfaces of the two rotating shafts are fixedly connected to two sprockets, and the four sprockets are arranged in a rectangular array inside the base frame, the two sprockets are laterally connected through a chain transmission, the chain links are rotatably connected to a connecting rod, the end of the connecting rod away from the chain is fixedly connected to a placing plate, the upper surface of the base frame is fixedly connected to a support column, and the top of the support column is fixedly connected to a nutrient solution barrel.

[0004] The Dendrobium officinale tissue culture filling device is provided with a reducer, a sprocket, a placement plate and a solenoid valve, so that the reducer is started to drive the sprocket to move the placement plate, and the solenoid valve is started to complete the filling of the culture bottle when the placement plate moves to the bottom of the liquid outlet pipe. The automated filling reduces the labor intensity of workers and greatly increases the work efficiency. However, there are still some technical shortcomings: 1. The use of liquid culture in tissue culture is considered to be an ideal solution to reduce costs and achieve automation, but liquid culture can easily lead to excessive water absorption and "vitrification" of plants. Forced ventilation of tissue culture containers is not only easy to cause large-scale pollution, but also increases the complexity of automated operations; 2. There is a problem of nutrient solution dripping. When a culture bottle is filled, before the next filling, a small amount of nutrient solution will drip from the filling head, which can easily dirty the operating table. Long-term retention of liquid may cause damage to the device components and cause a certain amount of nutrient solution waste; 3. The degree of automation is low, and only tissue culture filling can be achieved. It lacks functions such as plant tissue shearing and automatic packaging offline. The overall efficiency of automated tissue culture is not high. Summary of the invention

[0005] 1. Technical problem to be solved by the invention: The present invention provides a plant automated tissue culture device to solve the technical problems existing in the above-mentioned background technology.

[0006] 2. Technical solution: To achieve the above object, the technical solution provided by the present invention is: a plant automated tissue culture device, comprising a frame, the frame is provided with a station switching mechanism, the station switching mechanism comprises a first motor, the output shaft end of the first motor extends to the top of the frame in a vertical direction and is fixedly mounted with a disc; The disc is driven to rotate by the first motor and sequentially passes through an upper line pot taking mechanism, a nutrient solution injection mechanism, a plant tissue conveyor, a plant tissue shearing mechanism, an automatic packaging machine, a lower line pot taking mechanism and a lower line conveyor arranged on the frame.

[0007] Furthermore, a limiting frame is arranged on the top of the disk, and the limiting frames are multiple in number and arranged along the circumference of the disk, and the limiting frames match the workstation holes opened on the disk.

[0008] Furthermore, the online pot-taking mechanism includes a first support frame, which is fixedly connected to the frame and has a plurality of limit rods extending in the vertical direction on the top. The plurality of limit rods form a storage cavity, and the storage cavity matches the limit frame, and has tissue culture pots stacked inside and a material discharge assembly at the bottom.

[0009] Furthermore, the unloading assembly includes a first guide rail arranged on a first support frame, a limit plate is slidably installed in the first guide rail, and a second motor is installed at one end and a first roller is installed at the other end, the output shaft of the second motor and the first roller are connected by a first pulley, the belt of the first pulley is fixedly connected to the limit plate, and the limit plate is connected to a limit opening slightly smaller than the tissue culture bowl, and a unloading opening slightly larger than the tissue culture bowl is opened.

[0010] Furthermore, the nutrient solution injection mechanism includes a second support frame, which is fixedly connected to the frame and equipped with an injection nozzle matching the tissue culture bowl, and the liquid inlet of the injection nozzle is connected to the infusion tube and the diaphragm pump and is connected to the nutrient solution barrel.

[0011] Furthermore, the plant tissue shearing mechanism includes a third supporting frame, the third supporting frame is fixedly connected to the frame and is equipped with a second guide rail, the extension direction of the second guide rail is the same as the feeding direction of the plant tissue conveyor, a third roller is slidably installed in the second guide rail, and a third motor is installed at one end and a fourth roller is provided at the other end, the output shaft of the third motor and the fourth roller are connected by a second pulley, a shearing table is fixedly installed on the belt of the second pulley, and the third roller is mounted on the shearing table.

[0012] Furthermore, the shearing table is equipped with a sensor, and a sliding rod extending in the vertical direction is provided at the top edge, a first movable frame is slidably installed on the sliding rod, the first movable frame is threadedly connected to a first lead screw, the first lead screw is connected to a first coupling, the first coupling is connected to a sixth motor installed on the third supporting frame, the first movable frame is equipped with a fourth motor, the output end of the fourth motor extends to the bottom of the first movable frame, and a shearing tool is fixedly installed thereon.

[0013] Furthermore, a fifth motor and a pressing roller are also provided on the first movable frame. There are two pressing rollers, which are relatively distributed on both sides of the shearing tool. The rotating shafts of the two pressing rollers are fixedly connected with second rollers, and the two second rollers are connected to the sixth motor through a third pulley.

[0014] Furthermore, the offline bowl-taking mechanism includes a fourth support frame, the fourth support frame is fixedly connected to the frame and is provided with a fourth guide rail whose extension direction intersects with the offline conveyor, a ninth motor is provided at one end of the fourth guide rail and a fifth roller is provided at the other end, the output shaft and the fifth roller of the ninth motor are connected through a fourth pulley, the belt of the fourth pulley is fixedly connected with a third guide rail, the third guide rail extends in a vertical direction and is slidably mounted with a second movable frame, a second lead screw is threadedly connected to the second movable frame and a clamping mechanism is provided at the bottom, the second lead screw is connected to a second coupling, and the second coupling is connected to an eighth motor arranged on the third guide rail.

[0015] Furthermore, the clamping mechanism includes two symmetrically distributed clamping jaws, the two clamping jaws are meshed with each other via gears, and a seventh motor is mounted on the end of the rotating shaft of one of the clamping jaws.

[0016] 3. Beneficial effects: Compared with the prior art, the technical solution provided by the present invention has the following beneficial effects: the station switching mechanism is used to accurately rotate the tissue culture pot to the process position corresponding to other structures; the upper line pot taking mechanism is used to drop multiple stacked tissue culture pots into the holes on the station switching mechanism one by one; the nutrient solution injection mechanism is used for quantitative injection of nutrient solution; the plant tissue conveying mechanism is used to convey the plant tissue to be sliced ​​to the plant tissue shearing mechanism; the automatic packaging machine is used to seal the pot mouth of the culture pot; the lower line pot taking mechanism is used to take the packaged culture pot away from the station switching mechanism and convey it to the lower line conveying mechanism; the lower line conveying mechanism conveys the tissue culture pot out of the automated tissue culture device, and the entire process from automatic pot taking, automatic transmission, automatic shearing of plant tissue, automatic inoculation, and automatic packaging to the lower line is automatically operated, thereby reducing the labor intensity of manual tissue culture and effectively improving the efficiency of tissue culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the structure of the workstation switching mechanism of the present invention; Figure 3 It is a structural schematic diagram of the upper line bowl taking mechanism of the present invention; Figure 4 It is a schematic diagram of the structure of the limiting plate of the present invention; Figure 5 This is a schematic diagram of the structure of the nutrient solution injection mechanism of the present invention; Figure 6 The plant tissue shearing mechanism of the present invention is schematically shown in FIG. Figure 1 ; Figure 7 The plant tissue shearing mechanism of the present invention is schematically shown in FIG. Figure 2 ; Figure 8 It is a structural schematic diagram of the off-line bowl-taking mechanism of the present invention.

[0018] Reference numerals: 1. Workstation switching mechanism; 11. First motor; 12. Disc; 13. Limiting frame; 14. Workstation hole; 2. On-line potting mechanism; 21. First support frame; 22. Second motor; 23. Tissue culture pot; 24. Limiting rod; 25. First guide rail; 26. First pulley; 27. Limiting plate; 271. Limiting port; 272. Feeding port; 28. First roller; 3. Nutrient solution injection mechanism; 31. Injection nozzle; 32. Second support frame; 33. Nutrient solution barrel; 34. Diaphragm pump; 4. Plant tissue conveyor; 5. Plant tissue shearing mechanism; 501. Third motor; 502. Second pulley; 503. Second guide rail; 504. Sliding rod; 505. First moving frame; 506. Shearing tool; 507. Fourth motor ;508, the fifth motor;509, the third pulley;510, the second roller;511, the third roller;512, the shearing table;513, the sensor;514, the pressing roller;515, the first coupling;516, the sixth motor;517, the first screw;518, the third support frame;519, the fourth roller;6, the automatic packaging machine;7, the offline bowl-taking mechanism;701, the clamp;702, the seventh motor;703, the fifth roller;704, the fourth pulley;705, the second moving frame;706, the third guide rail;707, the eighth motor;708, the second coupling;709, the second screw;710, the fourth guide rail;711, the ninth motor;712, the fourth support frame;8, the offline conveyor;9, the frame. DETAILED DESCRIPTION

[0019] To facilitate understanding of the present invention, the present invention will be described more comprehensively below with reference to the relevant drawings. Several embodiments of the present invention are shown in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0020] In the description of the present invention, it is to be understood that the terms “center”, “longitudinal”, “lateral”, “length”, “width”, “thickness”, “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “page”, “bottom”, “inside”, “outside”, “clockwise”, “counterclockwise”, etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0021] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0022] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed", "provided with", etc. should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be internal communication between two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. Example

[0023] See attached Figure 1-8 , a plant automated tissue culture device, comprising a frame 9, on which a station switching mechanism 1 is arranged, wherein the station switching mechanism 1 comprises a first motor 11, wherein an output shaft end of the first motor 11 extends vertically to the top of the frame 9 and is fixedly mounted with a disc 12; The disc 12 is driven to rotate by the first motor 11 and passes through the upper line pot taking mechanism 2, the nutrient solution injection mechanism 3, the plant tissue conveyor 4, the plant tissue shearing mechanism 5, the automatic packaging machine 6, the lower line pot taking mechanism 7 and the lower line conveyor 8 arranged on the frame 9 in sequence.

[0024] In this embodiment, after the first motor 11 is started, it drives the disc 12 to rotate. The disc 12 will first pass through the online pot-taking mechanism 2. The online pot-taking mechanism 2 places the tissue culture pot 23 on the disc 12. The tissue culture pot 23 rotates with the disc 12 to the nutrient solution injection mechanism 3 to inject nutrient solution. Then the tissue culture pot 23 arrives under the plant tissue shearing mechanism 5. The plant tissue conveyor 4 will transport the plant tissue to the plant tissue shearing mechanism 5 and cut it and drop it into the tissue culture pot 23. Then the tissue culture pot 23 containing the plant tissue is packaged by the automatic packaging machine 6. Finally, the packaged tissue culture pot 23 is sent to the offline conveyor 8 for transportation and departure through the offline pot-taking mechanism 7. The entire process from automatic pot-taking, automatic transmission, automatic shearing of plant tissue, automatic inoculation, and automatic packaging to the offline process is automatically operated, which reduces the labor intensity of manual tissue culture and effectively improves the efficiency of tissue culture.

[0025] In the above embodiment, a limit frame 13 is provided on the top of the disk 12. The limit frames 13 are multiple and arranged along the circumference of the disk 12. The limit frames 13 match the work holes 14 provided on the disk 12 and are used to limit the tissue culture bowl 23 to avoid displacement during rotation.

[0026] The online pot-taking mechanism 2 includes a first support frame 21, which is fixedly connected to the frame 9, and a plurality of limit rods 24 extending in the vertical direction are arranged on the top, and the plurality of limit rods 24 form a storage cavity, and the storage cavity matches the limit frame 13, and tissue culture pots 23 are stacked inside, and a material discharge assembly is arranged at the bottom.

[0027] The material discharge assembly includes a first guide rail 25 arranged on a first support frame 21, a limit plate 27 is slidably installed in the first guide rail 25, and a second motor 22 is installed at one end, and a first roller 28 is installed at the other end, the output shaft of the second motor 22 and the first roller 28 are connected through a first pulley 26, the belt of the first pulley 26 is fixedly connected to the limit plate 27, and the limit plate 27 is connected to a limit opening 271 slightly smaller than the tissue culture bowl 23, and a material discharge opening 272 slightly larger than the tissue culture bowl 23.

[0028] In this embodiment, a plurality of tissue culture bowls 23 are stacked and placed in a storage cavity formed by four limiting rods 24. When the limiting frame 13 on the station switching mechanism 1 rotates to the bottom of the tissue culture bowl 23, the second motor 22 starts to drive the first pulley 26 to operate, so that the discharge port 272 on the limiting plate 27 can be pulled below the tissue culture bowl 23. The edge of the discharge port 272 touches the tissue culture bowl 23, breaking the adhesion between the bowl bodies of the tissue culture bowl 23, and the tissue culture bowl 23 falls into the limiting frame 13 through the discharge port 272. Similarly, after the tissue culture bowl 23 falls, the second motor 22 reverses to drive the limiting plate 27 back to the starting position, so that the tissue culture bowl 23 can be limited by the limiting port 271 to prevent it from falling.

[0029] The nutrient solution injection mechanism 3 includes a second support frame 32 , which is fixedly connected to the frame 9 and is equipped with an injection nozzle 31 matching the tissue culture bowl 23 . The liquid inlet of the injection nozzle 31 is connected to the infusion tube and the diaphragm pump 34 and is connected to the nutrient solution barrel 33 .

[0030] In this embodiment, after the online bowl is taken, the tissue culture bowl 23 rotates to the position directly below the injection nozzle 31, and the diaphragm pump 34 is started to transport the nutrient solution in the nutrient solution barrel 33 to the injection nozzle 31 through the infusion tube, and then the nutrient solution is added into the tissue culture bowl 23 through the injection nozzle 31.

[0031] In the above embodiment, the nutrient solution in the nutrient solution barrel 33 is atomized into droplets containing mixed gas and nutrient solution by ultrasonic wave, which provides nutrients and dissolved oxygen for the culture, and effectively solves the shortcomings of liquid culture.

[0032] The plant tissue shearing mechanism 5 includes a third support frame 518, which is fixedly connected to the frame 9 and is equipped with a second guide rail 503. The extension direction of the second guide rail 503 is the same as the feeding direction of the plant tissue conveyor 4. A third roller 511 is slidably installed in the second guide rail 503, and a third motor 501 is installed at one end and a fourth roller 519 is provided at the other end. The output shaft of the third motor 501 and the fourth roller 519 are connected by a second pulley 502. A shearing table 512 is fixedly installed on the belt of the second pulley 502, and the third roller 511 is assembled on the shearing table 512.

[0033] The shearing table 512 is equipped with a sensor 513, and a sliding bar 504 extending in the vertical direction is provided at the top edge, a first movable frame 505 is slidably installed on the sliding bar 504, the first movable frame 505 is threadedly connected with a first screw 517, the first screw 517 is connected with a first coupling 515, the first coupling 515 is connected to a sixth motor 516 installed on the third supporting frame 518, the first movable frame 505 is equipped with a fourth motor 507, the output end of the fourth motor 507 extends to the bottom of the first movable frame 505, and a shearing tool 506 is fixedly installed.

[0034] The first movable frame 505 is also provided with a fifth motor 508 and a pressing roller 514. There are two pressing rollers 514, which are relatively distributed on both sides of the shearing tool 506. The rotating shafts of the two pressing rollers 514 are fixedly connected with second rollers 510. The two second rollers 510 are connected to the fifth motor 508 via a third pulley 509.

[0035] In this embodiment, when the tissue culture bowl 23 rotates to the position directly below the small hole on the workbench of the shearing table 512, the shearing table 512 moves along the second guide rail 503 to the bottom of the exit end of the plant tissue conveyor 4, and the plant tissue falls from the plant tissue conveyor 4 to the working surface of the shearing table 512, and then the shearing table 512 returns to the top of the tissue culture bowl 23. At the same time, the sixth motor 516 drives the first screw 517 to rotate, so that the first movable frame 505 moves downward until the pressing roller 514 touches the plant tissue. Then the fifth motor 508 drives the pressing roller 514 to rotate, and adjusts the plant tissue to a position that completely covers the small hole on the workbench of the shearing table 512. The sensor 513 detects whether it has reached the specified position. After reaching the specified position, the fourth motor 507 drives the shearing tool 506 to move downward to complete the shearing, and the separated plant tissue will fall from the small hole on the shearing table 512 into the tissue culture bowl 23.

[0036] In the above embodiment, after the plant tissue is sheared, the shearing table 512 moves along the second guide rail 503 to a fixed position between the plant tissue conveying mechanism 4 and the plant tissue shearing mechanism 5, where there is a waste collection bucket. The fifth motor 508 reverses, driving the pressing roller 514 to reverse, and discharges the sheared waste out of the shearing table 512.

[0037] The offline bowl-taking mechanism 7 includes a fourth support frame 712, which is fixedly connected to the frame 9 and is provided with a fourth guide rail 710 whose extension direction intersects with the offline conveyor 8. A ninth motor 711 is provided at one end of the fourth guide rail 710, and a fifth roller 703 is provided at the other end. The output shaft of the ninth motor 711 and the fifth roller 703 are connected via a fourth pulley 704. A third guide rail 706 is fixedly connected to the belt of the fourth pulley 704. The third guide rail 706 extends in a vertical direction and is slidably mounted with a second movable frame 705. A second lead screw 709 is threadedly connected to the second movable frame 705, and a clamping mechanism is provided at the bottom. The second lead screw 709 is connected to a second coupling 708, and the second coupling 708 is connected to an eighth motor 707 provided on the third guide rail 706.

[0038] The clamping mechanism includes two symmetrically distributed clamping jaws 701 , the two clamping jaws 701 are meshed with each other via gears, and a seventh motor 702 is mounted on the end of the rotating shaft of one of the clamping jaws 701 .

[0039] In this embodiment, after the tissue culture bowl 23 is packaged by the automatic packaging machine 6, the station switching mechanism 1 rotates to the bottom of the clamp 701, and the eighth motor 707 drives the second lead screw 709 to move the second movable frame 705 downward. At the same time, the seventh motor 702 drives the clamp 701 to loosen. When the clamp 701 moves to the position of the tissue culture bowl 23, the seventh motor 702 drives the clamp 701 to tighten. After clamping the tissue culture bowl 23, the seventh motor 702 reverses and clamps the tissue culture bowl 23 to move upward. After the tissue culture bowl 23 rises to a certain height, the ninth motor 711 drives the fourth pulley pulley 704 to drive the third guide rail 706 to move away from the station switching mechanism 1 until it moves to the top of the offline conveying mechanism 8, and then the eighth motor 707 drives the second lead screw 709 to move the tissue culture bowl 23 downward to a certain position, and the seventh motor 702 drives the clamp 701 to loosen, and the tissue culture bowl 23 falls on the offline conveying mechanism 8.

[0040] The above-described embodiments only express a certain implementation mode of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the patent scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be based on the attached claims.

[0041] It should be noted that the above contents belong to the technical cognition scope of the inventor. Since the technical contents in this field are vast and too complicated, the above contents of this application do not necessarily constitute prior art.

Claims

1. A plant automated tissue culture device, characterized in that: The machine comprises a frame (9), wherein a workstation switching mechanism (1) is arranged on the frame (9), wherein the workstation switching mechanism (1) comprises a first motor (11), wherein an output shaft end of the first motor (11) extends in a vertical direction to the top of the frame (9) and is fixedly mounted with a disc (12); The disc (12) is driven to rotate by the first motor (11) and sequentially passes through an upper line pot taking mechanism (2), a nutrient solution injection mechanism (3), a plant tissue conveyor (4), a plant tissue shearing mechanism (5), an automatic packaging machine (6), a lower line pot taking mechanism (7) and a lower line conveyor (8) arranged on the frame (9).

2. The plant automated tissue culture device according to claim 1, characterized in that: A limit frame (13) is provided on the top of the circular disk (12); the limit frames (13) are multiple in number and arranged along the circumference of the circular disk (12); the limit frames (13) match the workstation holes (14) provided on the circular disk (12).

3. The plant automated tissue culture device according to claim 2, characterized in that: The upper line pot-taking mechanism (2) comprises a first support frame (21), the first support frame (21) is fixedly connected to the frame (9), and a plurality of limit rods (24) extending in the vertical direction are arranged on the top, and the plurality of limit rods (24) form a storage cavity, the storage cavity matches the limit frame (13), and tissue culture pots (23) are stacked inside, and a material discharge assembly is arranged at the bottom.

4. The plant automated tissue culture device according to claim 3, characterized in that: The material discharge assembly comprises a first guide rail (25) arranged on a first support frame (21), a limit plate (27) is slidably installed in the first guide rail (25), a second motor (22) is mounted on one end, and a first roller (28) is mounted on the other end, an output shaft of the second motor (22) and the first roller (28) are connected via a first pulley (26), a belt of the first pulley (26) is fixedly connected to the limit plate (27), and a limit opening (271) slightly smaller than the tissue culture bowl (23) and a material discharge opening (272) slightly larger than the tissue culture bowl (23) are communicated and opened on the limit plate (27).

5. The plant automated tissue culture device according to claim 2, characterized in that: The nutrient solution injection mechanism (3) comprises a second support frame (32), the second support frame (32) is fixedly connected to the frame (9) and is equipped with an injection nozzle (31) matching the tissue culture pot (23), the liquid inlet of the injection nozzle (31) is connected to the infusion tube and the diaphragm pump (34) and is connected to the nutrient solution barrel (33).

6. The plant automated tissue culture device according to claim 1, characterized in that: The plant tissue shearing mechanism (5) comprises a third support frame (518), the third support frame (518) is fixedly connected to the frame (9) and is equipped with a second guide rail (503), the extension direction of the second guide rail (503) is the same as the feeding direction of the plant tissue conveyor (4), a third roller (511) is slidably installed in the second guide rail (503), and a third motor (501) is installed at one end and a fourth roller (519) is provided at the other end, the output shaft of the third motor (501) and the fourth roller (519) are connected via a second pulley (502), a shearing table (512) is fixedly installed on the belt of the second pulley (502), and the third roller (511) is mounted on the shearing table (512).

7. The plant automated tissue culture device according to claim 6, characterized in that: The shearing table (512) is equipped with a sensor (513), and a sliding rod (504) extending in the vertical direction is arranged at the top edge, a first movable frame (505) is slidably mounted on the sliding rod (504), the first movable frame (505) is threadedly connected to a first lead screw (517), the first lead screw (517) is connected to a first coupling (515), the first coupling (515) is connected to a sixth motor (516) mounted on a third support frame (518), the first movable frame (505) is equipped with a fourth motor (507), the output end of the fourth motor (507) extends to the bottom of the first movable frame (505), and a shearing tool (506) is fixedly mounted thereon.

8. The plant automated tissue culture device according to claim 7, characterized in that: The first movable frame (505) is also provided with a fifth motor (508) and a pressing roller (514). There are two pressing rollers (514) which are relatively distributed on both sides of the shearing tool (506). The rotating shafts of the two pressing rollers (514) are fixedly connected with second rollers (510). The two second rollers (510) are connected to the sixth motor (508) via a third pulley (509).

9. The plant automated tissue culture device according to claim 1, characterized in that: The off-line bowl-taking mechanism (7) comprises a fourth support frame (712), the fourth support frame (712) being fixedly connected to the frame (9) and being provided with a fourth guide rail (710) whose extension direction intersects with the off-line conveyor (8), a ninth motor (711) being provided at one end of the fourth guide rail (710) and a fifth roller (703) being provided at the other end, the output shaft of the ninth motor (711) and the fifth roller (703) being connected via a fourth pulley (704), the fourth A third guide rail (706) is fixedly connected to the belt of the pulley (704); the third guide rail (706) extends in a vertical direction and is slidably mounted with a second movable frame (705); a second lead screw (709) is threadedly connected to the second movable frame (705) and a clamping mechanism is arranged at the bottom; the second lead screw (709) is connected to a second coupling (708); and the second coupling (708) is connected to an eighth motor (707) arranged on the third guide rail (706).

10. The plant automated tissue culture device according to claim 9, characterized in that: The clamping mechanism comprises two symmetrically distributed clamping jaws (701), the two clamping jaws (701) are meshed with each other via gears, and a seventh motor (702) is mounted on the end of a rotating shaft of one of the clamping jaws (701).

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