Predatory mite functional release medium as well as preparation method and use method thereof

Abstract

The invention belongs to the field of biological control of agricultural pests, and particularly relates to a predatory mite functional release medium as well as a preparation method and a use method thereof. The predatory mite functional release medium comprises entomogenous fungi, silicon dioxide, kieselguhr and filler, the entomogenous fungi and the kieselguhr can control various pest mites and pests and have no negative influence on the predatory mites, and by means of the release medium, the prevention and control effect on the predatory mites can be improved, and the predatory mites can be prevented and controlled. The composition has certain prevention and control effects on aphids, thrips and other pests, the prevention and control object range of original predatory mite products can be widened, and integrated prevention and control of various pest mites and pests are achieved. Besides, the use method of the release medium is simple, the predatory mite functional release medium not only can ensure the application dosage requirement of biocontrol microorganisms, but also can reduce the field pesticide application frequency, and the prevention and control cost is reduced while the prevention and control efficiency and the prevention and control effect are improved.

Description

Technical Field

[0001] The invention belongs to the field of biological control of agricultural pests, and specifically relates to a functional release medium for predatory mites and a preparation method and a use method thereof. Background Art

[0002] For a long time, due to the abuse and misuse of pesticides, many small sap-sucking pests such as spider mites, thrips, whiteflies, aphids, etc. have been rampant, harming crops, and often multiple pests occur together, making chemical control increasingly difficult. In contrast, predatory mites have an effective control effect on harmful mites and pests due to their small size, rapid development, large reproduction, strong predation ability, and occupying similar ecological niches with pests. Some predatory mite species with super strong predation ability, such as the Chilean phytoseiid mite, can even achieve a rapid control effect similar to that of pesticides.

[0003] Phytoseiidae is a specialized predator of spider mites, with the characteristics of large prey volume, strong reproductive capacity, and rapid control. This mite has been introduced and commercialized in many countries. The use of Phytoseiidae to control spider mites on cotton, vegetables, fruit trees and flowers has achieved significant control effects. However, this mite is a narrow-grazing mite that feeds on spider mites and is ineffective against pests such as thrips and aphids. In the case of mixed occurrence of multiple harmful mites and pests, it needs to be used in combination with other natural enemies or pesticides to achieve an integrated control effect.

[0004] When applying predatory mites over a large area, the flooding release method is often adopted. That is, commercially produced predatory mite products are mixed with media such as vermiculite and wheat bran, and then spread on crops manually or using a special spreading machine. Conventional release media are single-component vermiculite, wheat bran or sawdust, which can only play the role of filling, moisturizing, and spreading evenly, and have no other functions. Summary of the invention

[0005] Based on the above technical background, the main purpose of the present invention is to provide a functional release medium for predatory mites and a preparation method and a use method thereof, so as to overcome the deficiencies in the prior art.

[0006] In order to achieve the above-mentioned invention object, the technical solution adopted by the present invention includes:

[0007] The first aspect of the present invention is to provide a predatory mite functional release medium, the predatory mite functional release medium comprising entomogenous fungi, silicon dioxide, diatomaceous earth and filler;

[0008] The mass ratio of the entomogenous fungus, silicon dioxide, diatomaceous earth and filler is (1-5): (0.5-2): (20-40): (50-80).

[0009] Preferably, the mass ratio of the entomogenous fungus, silicon dioxide, diatomaceous earth and filler is 4:1:30:65.

[0010] Preferably, the entomogenous fungi are selected from one or two of the entomogenous fungi of Beauveria bassiana and Metarhizium anisopliae.

[0011] Preferably, the filler is selected from one or more of edible fungus residue, vermiculite and wheat bran.

[0012] More preferably, the filler is edible fungus residue.

[0013] More preferably, the mass ratio of the entomogenous fungus spore powder of Beauveria bassiana, the entomogenous fungus spore powder of Metarhizium anisopliae, silicon dioxide, diatomaceous earth and edible fungus residue is 2:2:1:30:65;

[0014] Or, the mass ratio of the Beauveria bassiana entomogenous fungus spore powder, silicon dioxide, diatomaceous earth and edible fungus residue is 4:1:30:65;

[0015] Or, the mass ratio of the green muscardine entomogenous fungus spore powder, silicon dioxide, diatomaceous earth and edible fungus residue is 4:1:30:65.

[0016] The second aspect of the present invention is to provide a method for preparing the functional release medium of predatory mites according to the first aspect of the present invention, the preparation method comprising the following steps:

[0017] Step 1, sterilizing and drying diatomaceous earth and filler;

[0018] Step 2: Mix the sterilized and dried diatomaceous earth and filler, entomogenous fungi and silicon dioxide to obtain a functional release medium for predatory mites.

[0019] Preferably, in step 1,

[0020] Sterilize the diatomaceous earth and filler at 100°C for 60 to 120 minutes;

[0021] Dry the sterilized diatomaceous earth and filler at 50-60°C for 45-90 minutes.

[0022] The third aspect of the present invention is to provide a method for using the predatory mite functional release medium according to the first aspect of the present invention, the method comprising:

[0023] Investigate the number of spider mites in the release field, the benefit-harm ratio of predatory mites to spider mites is 1:10-15, the application dosage of predatory mite functional release medium is 500g-1000g / mu, mix the predatory mites and the predatory mite functional release medium thoroughly according to this dosage, and spread them on the crop leaves. Choose a cloudy day, evening or early morning as the time for spreading, and avoid using any chemical pesticides 10 days before and after spreading.

[0024] Preferably, the predatory mite is Phytoseiidae chilensis.

[0025] The beneficial effects of the present invention are:

[0026] (1) The functional release medium for predatory mites of the present invention comprises entomogenous fungi, silicon dioxide, diatomaceous earth and filler, wherein the entomogenous fungi and diatomaceous earth have control effects on various harmful mites and pests, and have no negative impact on the mortality, predation and yield of predatory mites, titanium dioxide has anti-ultraviolet function, and plays a protective role on fungal spores and predatory mites, the use of the release medium not only has a filling effect, but also can improve the control effect of predatory mites, the release medium also has a certain control and control effect on other pests such as aphids and thrips, can broaden the scope of control objects of the original predatory mite products, realize the integrated control of various harmful mites and pests, improve the control efficiency, and reduce the control cost. It was found through experiments that compared with the use of conventional release medium, the control effect of the release medium on spider mites was improved by 12.05%, and the control effect on aphids reached 85.76% or more.

[0027] (2) Existing bacteria-carrying predatory mite products only carry biocontrol microorganisms on the surface of predatory mites, and the amount of bacteria carried cannot guarantee the dosage requirements of biocontrol bacteria; although the application dosage of biocontrol bacteria can be guaranteed by spreading biocontrol bacteria and predatory mites separately, the number of pesticide application operations is increased, which increases the cost of prevention and control; the functional release medium for predatory mites described in the present invention is mixed with predatory mites and applied to the field at the same time, and can be spread manually or by a professional spreading machine. During the spreading process, the biocontrol microorganisms are directly introduced into the release environment along with diatomaceous earth, etc. At the same time, the biocontrol microorganisms can also be carried on the surface of predatory mites and spread to gaps that the release medium cannot reach, thereby further improving the prevention and control effect. The functional release medium for predatory mites can not only guarantee the dosage requirements of biocontrol microorganisms, but also reduce the number of field pesticide applications, thereby improving the prevention and control efficiency and reducing the prevention and control cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 A line graph showing the change in mortality of Tetranychus urticae over time after treatment with Metarhizium anisopliae in Experimental Example 1;

[0029] Figure 2 A line graph showing the change in mortality of Phytoseiius chilensis mites over time after treatment with Metarhizium anisopliae in Experimental Example 1;

[0030] Figure 3 The figure shows the average daily prey volume of a single Phytoseiius chilensis mite after being treated with Metarhizium anisopliae in Experimental Example 1;

[0031] Figure 4 The figure shows the average daily egg-laying capacity of a single Phytoseiius chilensis mite after treatment with Metarhizium anisopliae in Experimental Example 1. DETAILED DESCRIPTION

[0032] The present invention will be described in detail below, and the characteristics and advantages of the present invention will become clearer and more distinct with the description.

[0033] The existing conventional release matrix for predatory mites is a single component of vermiculite, wheat bran or sawdust, etc., which can only play the role of filling, moisturizing and spreading evenly, but has no other functions. The present invention selects entomogenous fungi that have good control effects on pests such as thrips, whiteflies, aphids, etc., but have no negative impact on Chilean phytoseiid mites, and adds titanium dioxide, diatomaceous earth and fillers at the same time.

[0034] The first aspect of the present invention is to provide a predatory mite functional release medium, which comprises entomogenous fungi, silicon dioxide, diatomaceous earth and fillers.

[0035] The mass ratio of the entomogenous fungus, silicon dioxide, diatomaceous earth and filler is (1-5): (0.5-2): (20-40): (50-80).

[0036] Preferably, the mass ratio of the entomogenous fungus, silicon dioxide, diatomaceous earth and filler is 4:1:30:65.

[0037] According to a preferred embodiment of the present invention, the entomogenous fungi are selected from one or two of Beauveria bassiana entomogenous fungi and Metarhizium anisopliae entomogenous fungi, preferably a mixture of Beauveria bassiana entomogenous fungi and Metarhizium anisopliae entomogenous fungi.

[0038] Beauveria bassiana and Metarhizium anisopliae have good control effects on pests such as spider mites, thrips, whiteflies, aphids, etc., and have no adverse effects on the predation, reproductive capacity, and lifespan of the Chilean phytoseiid mite.

[0039] The entomogenous fungus is preferably entomogenous fungus spore powder, and the mass ratio of Beauveria bassiana entomogenous fungus spore powder to Metarhizium anisopliae entomogenous fungus spore powder is preferably 1:1.

[0040] The filler is selected from one or more of edible fungus residue, vermiculite, and wheat bran, and is preferably edible fungus residue.

[0041] According to a preferred embodiment of the present invention, the mass ratio of the entomological fungus spore powder of Beauveria bassiana, the entomological fungus spore powder of Metarhizium anisopliae, silicon dioxide, diatomaceous earth and edible fungus residue is 2:2:1:30:65.

[0042] Or, the mass ratio of the Beauveria bassiana entomogenous fungus spore powder, silicon dioxide, diatomaceous earth and edible fungus residue is 4:1:30:65.

[0043] Or, the mass ratio of the green muscardine entomogenous fungus spore powder, silicon dioxide, diatomaceous earth and edible fungus residue is 4:1:30:65.

[0044] The second aspect of the present invention is to provide a method for preparing the functional release medium of predatory mites according to the first aspect of the present invention, the preparation method comprising the following steps:

[0045] Step 1, sterilizing and drying diatomaceous earth and filler;

[0046] Step 2: Mix the sterilized and dried diatomaceous earth and filler, entomogenous fungi and silicon dioxide to obtain a functional release medium for predatory mites.

[0047] The above steps are described in detail below.

[0048] In step 1, the diatomaceous earth and the filler are sterilized at 100° C. for 60 to 120 min. Preferably, the diatomaceous earth and the filler are sterilized at 100° C. for 90 min.

[0049] The sterilized diatomaceous earth and filler are dried at 50-60° C. for 45-90 min. Preferably, the sterilized diatomaceous earth and filler are dried at 55° C. for 60 min, and the water content after drying is less than 5%.

[0050] In step 2, the sterilized and dried diatomaceous earth and filler are mixed with entomogenic fungi and silicon dioxide in a ratio of 30:65:4:1, and after mixing, the entomogenic fungi spore content in the functional release medium of predatory mites is greater than 3 billion spores / g.

[0051] The third aspect of the present invention is to provide a method for using the predatory mite functional release medium according to the first aspect of the present invention, the method comprising:

[0052] First, investigate the number of spider mites in the release field, prepare predatory mites according to the ratio of benefit-harm of predatory mites to spider mites of 1:10-15, preferably 1:10, and the application dosage of predatory mite functional release medium is 500g-1000g / mu, preferably 1000g / mu. Mix the predatory mites and predatory mite functional release medium according to this usage amount, and spread them on the crop leaves by hand or professional spreading machine. The time of spreading should be cloudy, evening or early morning, avoid releasing and spreading on rainy days, strong winds and strong midday sunshine, and avoid using any chemical pesticides 10 days before and after spreading.

[0053] The predatory mite is preferably Phytoseiidae chilensis.

[0054] Example

[0055] The present invention is further described below by specific examples, which are limited to illustrating the present invention but not to limiting the scope of the present invention. The raw materials used in the present invention are all purchased.

[0056] Example 1

[0057] The diatomaceous earth and edible fungus residue were sterilized at 100° C. for 90 minutes, and then dried at 55° C. for 60 minutes. After drying, the water content was less than 5% for subsequent use.

[0058] The spore powder of Beauveria bassiana entomogenous fungus, the spore powder of Metarhizium anisopliae entomogenous fungus, silicon dioxide, diatomaceous earth and edible fungus residue are mixed in a mass ratio of 2:2:1:30:65, and the spore content after mixing is greater than 3 billion spores / g, so as to obtain a functional release medium for predatory mites.

[0059] Example 2

[0060] The diatomaceous earth and edible fungus residue were sterilized at 100° C. for 90 minutes, and then dried at 55° C. for 60 minutes. After drying, the water content was less than 5% for subsequent use.

[0061] The spore powder of green muscardine entomogenous fungus, silicon dioxide, diatomaceous earth and edible fungus residue are mixed in a mass ratio of 4:1:30:65. After mixing, the spore content is greater than 3 billion spores / g, so as to obtain a functional release medium for predatory mites.

[0062] Example 3

[0063] The diatomaceous earth and edible fungus residue were sterilized at 100° C. for 90 minutes, and then dried at 55° C. for 60 minutes. After drying, the water content was less than 5% for subsequent use.

[0064] The spore powder of Beauveria bassiana entomogenous fungus, silicon dioxide, diatomaceous earth and edible fungus residue are mixed in a mass ratio of 4:1:30:65, and the spore content after mixing is greater than 3 billion spores / g, so as to obtain a functional release medium for predatory mites.

[0065] Example 4

[0066] The diatomaceous earth and edible fungus residue were sterilized at 100° C. for 60 minutes, and then dried at 55° C. for 60 minutes. After drying, the water content was less than 5% for subsequent use.

[0067] The entomological fungus spore powder of Beauveria bassiana, the entomological fungus spore powder of Metarhizium muscardine, silicon dioxide, diatomaceous earth and edible fungus residue are mixed in a mass ratio of 1:1:1:20:77, and the spore content after mixing is greater than 3 billion spores / g, so as to obtain a functional release medium for predatory mites.

[0068] Example 5

[0069] The diatomaceous earth and edible fungus residue were sterilized at 100° C. for 120 min, and then dried at 55° C. for 60 min. After drying, the water content was less than 5% for subsequent use.

[0070] The spore powder of entomogenous fungus Beauveria bassiana, the spore powder of entomogenous fungus Metarhizium muscardine, silicon dioxide, diatomaceous earth and edible fungus residue are mixed in a mass ratio of 1:1:2:40:56, and the spore content after mixing is greater than 3 billion spores / g, so as to obtain a functional release medium for predatory mites.

[0071] Experimental example

[0072] Experimental Example 1 Pathogenicity and Control Effect Test

[0073] 1. Test materials

[0074] Green muscardine comes from the Institute of Plant Protection, Jilin Academy of Agricultural Sciences. Studies have shown that this strain has good control effects on aphids and other pests.

[0075] The Chilean phytoseiid mite is an indoor population raised at the Institute of Plant Protection, Jilin Academy of Agricultural Sciences.

[0076] The predatory mite functional release medium described in Example 2 was used for testing.

[0077] 2. Test methods

[0078] 2.1 Pathogenicity of Metarhizium anisopliae to Tetranychus urticae

[0079] Spray inoculation was used to inoculate 10 8 Sporulations of Metarhizium anisopliae spores at spores / mL were sprayed onto adult female two-spotted spider mites of the same age. Each treatment was repeated 10 times, with a total of 100 spider mites. The control group was sprayed with a solution containing 0.05% Tween-80. After spraying, the culture dish was placed in an incubator with a humidity of 70%±5%, a temperature of (25±2)℃, and a photoperiod of 16L:8D. The number of dead two-spotted spider mites was observed and recorded daily, and the dead mites were picked out. The dead mites were picked out and placed in a culture dish for moisturizing culture, and it was observed whether Metarhizium anisopliae grew on the insect body, which was used as a basis for judging whether the insect was killed by Metarhizium anisopliae infection. The experiment was observed continuously for 10 days. The corrected mortality rate and lethal median time LT50 (i.e., the time required for Metarhizium anisopliae to kill half of the spider mites) of the two-spotted spider mite were calculated according to formula (1) using the obtained data.

[0080] Corrected mortality rate (%) = (treatment mortality rate - control mortality rate) / (1 - control mortality rate) × 100% formula (1);

[0081] 2.2 Pathogenicity of Metarhizium anisopliae to Phytoseiidae

[0082] Use a small brush to pick healthy and lively adult Phytoseiidae Chileensis mites of uniform size and place them in 10 8The samples were soaked in a suspension of green muscardine with spores / mL for 5 seconds and then taken out. The bacterial liquid around the mites was sucked off and placed in a small chamber composed of two transparent acrylic plates. One Chilean phytoseiid mite was placed in each chamber. The predatory mites in the control group were treated with a solution containing 0.05% Tween-80. Each treatment was repeated 4 times, with 15 Chilean phytoseiid mites in each repetition. The mortality of each treatment was recorded and supplemented with food every day. The observation was continued for 10 days, and the dead mites were removed and kept moist for culture. The mortality rate and dead mites rate were calculated according to the growth of the mycelium of the dead mites.

[0083] 2.3 Effects of Metarhizium anisopliae on predation by Phytoseiidae

[0084] Use a small brush to pick healthy and lively female adult Phytoseiidae Chileensis mites of uniform size and place them in 10 8 The plants were soaked in a suspension of Metarhizium anisopliae with spores / mL for 5 seconds, then taken out and the mites were removed. The bacterial liquid was placed on a leaf dish with 15 adult spider mites. The control group was treated with a solution containing 0.05% Tween-80. Food was supplemented every day and the number of preys preyed by predatory mites in different treatments was counted. The investigation was continued for 5 days, and 15 female adult mites were measured in each treatment.

[0085] 2.4 Effect of Metarhizium anisopliae on the egg production of Phytoseiidae

[0086] Use a small brush to pick healthy and active female adult Phytoseiidae Chileensis mites of uniform size and place them in 8 The samples were soaked in a suspension of Metarhizium anisopliae with spores / mL for 5 seconds, then taken out, and the bacterial liquid around the mites was removed and placed in a small chamber consisting of two transparent acrylic plates. One Chilean Phytoseiidae was placed in each chamber, and the control group of predatory mites was treated with a solution containing 0.05% Tween-80. The egg-laying amount of each treatment was observed and recorded regularly every day, and food was supplemented. The observation was continued for 5 days, and 15 female adult mites were measured in each treatment.

[0087] 2.5 Control effect of functional release medium of predatory mites

[0088] Spider mites and aphids were inoculated on greenhouse eggplants, and a test treatment was carried out one week later (the number of spider mites and aphids was 57.4 and 18.8 per plant on average). The test set up four treatment groups: predatory mites, predatory mites (the benefit-harm ratio of predatory mites to spider mites was 1:10) + medium (predatory mite functional release medium prepared in Example 2) 500g / mu, predatory mites (the benefit-harm ratio of predatory mites to spider mites was 1:10) + medium (predatory mite functional release medium prepared in Example 2) 1000g / mu and control group. The control group did not take any control measures. The benefit-harm ratio of Chilean phytoseiid mites to spider mites was 1:10. The number of spider mites and aphids was investigated on the 10th and 20th days after release, and the control effect was calculated.

[0089] 3. Test results

[0090] 2.1 Pathogenicity of Metarhizium anisopliae to Tetranychus urticae

[0091] Test results such as Figure 1 As shown by Figure 1 It can be seen that the mortality rate of two-spotted spider mites after spraying Metarhizium was significantly higher than that of the control, and gradually increased with the treatment time. Ten days after treatment, the mortality rate of two-spotted spider mites sprayed with Metarhizium reached 85%, and the corrected mortality rate was 80.13%. The lethal median time of Metarhizium to two-spotted spider mites was 4.158 days.

[0092] 2.2 Pathogenicity of Metarhizium anisopliae to Phytoseiidae

[0093] The results of the pathogenicity test of Metarhizium anisopliae to Phytoseiidae in Chile are as follows: Figure 2 As shown, from Figure 2 It can be seen that with the extension of the investigation time, the mortality rate of Chilean phytoseiid mites has a tendency to gradually increase, but during the same investigation period, there is no significant difference in the mortality rate of Chilean phytoseiid mites treated with green muscardine compared with the control.

[0094] 2.3 Effects of Metarhizium anisopliae on predation by Phytoseiidae

[0095] The predation amount of Phytoseiidae chilensis on Tetranychus urticae after different treatments Figure 3 After treatment with Metarhizium anisopliae, the total prey intake of Phytoseiius chilensis mites in 5 days was 18.57 mites, and the average daily prey intake of each mites was 4.08 mites; in the control treatment, the total prey intake of Phytoseiius chilensis mites in 5 days was 22 mites, and the average daily prey intake of each mites was 4.63 mites. There was no significant difference between the two (total prey intake t=0.869, F=0.394; average daily prey intake of each mites t=0.184, F=0.258).

[0096] 2.4 Effects of Metarhizium anisopliae on the reproduction of Phytoseiidae

[0097] The egg laying amount of Phytoseiidae chilensis after different treatments Figure 4 As shown, after treatment with Metarhizium anisopliae, the total egg laying amount of the Chilean Phytoseiidae mites in 5 days was 18.49, and the average daily egg laying amount of a single head was 3.62; in the control treatment, the total egg laying amount of the Chilean Phytoseiidae mites in 5 days was 17.93, and the average daily egg laying amount of a single head was 3.49. There was no significant difference between the two (total egg laying amount t = 0.679, F = 0.496; average daily egg laying amount of a single head t = 0.1708, F = 0.103).

[0098] 2.5 Control effect of functional release medium of predatory mites

[0099] The numbers of spider mites and aphids investigated at different periods are shown in Table 1.

[0100] Table 1

[0101]

[0102] As can be seen from Table 1, after using the functional release medium of predatory mites of the present invention to treat spider mites and aphids, the number of spider mites and aphids is significantly reduced. After the treatment with predatory mites + medium 500g / mu, the control effect after 10 days is increased by 5.55% compared with the treatment with predatory mites alone. After the treatment with predatory mites + medium 1000g / mu, the control effect after 10 days is increased by 12.05% compared with the treatment with predatory mites alone. At the same time, it also has a good control effect on aphids, and the control effect reaches more than 75%.

[0103] In summary, the green muscardine added to the functional release medium of predatory mites described in the present invention has strong pathogenicity to aphids and spider mites, but has no significant effect on the mortality, predation and yield of Phytoseiidae chilensis. This shows that the application of the functional release medium of predatory mites described in the present invention can not only improve the control effect of predatory mite products on spider mites, but also have a good control effect on aphids. The functional release medium of predatory mites broadens the scope of control objects of the original predatory mite products, can realize the integrated control of multiple harmful mites and pests, improve control efficiency, and reduce control costs.

[0104] The present invention has been described in detail above in conjunction with specific implementations and exemplary examples, but these descriptions cannot be understood as limiting the present invention. Those skilled in the art understand that, without departing from the spirit and scope of the present invention, a variety of equivalent substitutions, modifications or improvements may be made to the technical solution of the present invention and its implementation methods, all of which fall within the scope of the present invention. The scope of protection of the present invention shall be subject to the attached claims.

Claims

1. A functional release medium for predatory mites, characterized in that: The functional release medium for predatory mites comprises entomogenous fungi, silicon dioxide, diatomaceous earth and fillers; The mass ratio of the entomogenous fungus, silicon dioxide, diatomaceous earth and filler is (1-5): (0.5-2): (20-40): (50-80).

2. The predatory mite functional release medium according to claim 1, characterized in that: The mass ratio of the entomogenous fungus, silicon dioxide, diatomaceous earth and filler is 4:1:30:

65.

3. The predatory mite functional release medium according to claim 1, characterized in that: The entomogenous fungi are selected from one or two of the entomogenous fungi of Beauveria bassiana and Metarhizium anisopliae.

4. The predatory mite functional release medium according to claim 1, characterized in that: The filler is selected from one or more of edible fungus residue, vermiculite and wheat bran.

5. The predatory mite functional release medium according to claim 4, characterized in that: The filler is edible fungus residue.

6. The predatory mite functional release medium according to any one of claims 1 to 5, characterized in that: The mass ratio of the Beauveria bassiana entomogenous fungus spore powder, Metarhizium anisopliae entomogenous fungus spore powder, silicon dioxide, diatomaceous earth and edible fungus residue is 2:2:1:30:65; Or, the mass ratio of the Beauveria bassiana entomogenous fungus spore powder, silicon dioxide, diatomaceous earth and edible fungus residue is 4:1:30:65; Or, the mass ratio of the green muscardine entomogenous fungus spore powder, silicon dioxide, diatomaceous earth and edible fungus residue is 4:1:30:

65.

7. A method for preparing the functional release medium for predatory mites according to any one of claims 1 to 6, characterized in that: The preparation method comprises the following steps: Step 1, sterilizing and drying diatomaceous earth and filler; Step 2: Mix the sterilized and dried diatomaceous earth and filler, entomogenous fungi and silicon dioxide to obtain a functional release medium for predatory mites.

8. The preparation method according to claim 7, characterized in that: In step 1, Sterilize the diatomaceous earth and filler at 100°C for 60 to 120 minutes; Dry the sterilized diatomaceous earth and filler at 50-60°C for 45-90 minutes.

9. A method for using the predatory mite functional release medium according to any one of claims 1 to 6, characterized in that: The method of use includes: Investigate the number of spider mites in the release field, the benefit-harm ratio of predatory mites to spider mites is 1:10-15, the application dosage of predatory mite functional release medium is 500g-1000g / mu, mix the predatory mites and the predatory mite functional release medium thoroughly according to this dosage, and spread them on the crop leaves. Choose a cloudy day, evening or early morning as the time for spreading, and avoid using any chemical pesticides 10 days before and after spreading.

10. The method of use according to claim 9, characterized in that: The predatory mite is Phytoseiidae chilensis.

Images