Colorectal cancer tumor marker detection device

Abstract

The invention relates to the field of marker detection, in particular to a colorectal cancer tumor marker detection device which comprises a supporting box, the supporting box comprises a bottom plate and a plurality of transverse plates and longitudinal plates which are arranged on the bottom plate, the transverse plates and the longitudinal plates are vertically staggered to form a plurality of rows of placing grooves, and enzyme label strips are placed in the placing grooves; a sliding hole is formed in the end of each transverse plate, sliding grooves are formed in the portions, opposite to the containing grooves, of the sliding holes, and the sliding grooves communicate with the interiors of the sliding holes and the containing grooves. According to the invention, the designed supporting box is matched with the elisa strip, the push rod is arranged in the supporting box, and the push rod is matched with the limiting structure, so that the elisa strip is extruded and fixed in the placing groove, the space of the placing groove is vacated, the elisa strip can be conveniently taken out of the placing groove in an inclined state, and the operation of a detector on the detection process is facilitated.

Description

Technical Field

[0001] The present invention relates to the field of marker detection, and in particular to a device for detecting colorectal cancer tumor markers. Background Art

[0002] The detection of colorectal cancer tumor markers has important clinical significance for early diagnosis, treatment selection, efficacy evaluation and prognosis judgment. Common colorectal cancer tumor markers include CEA (carcinoembryonic antigen), CA19-9 (carbohydrate antigen 19-9), CA72-4 (carbohydrate antigen 72-4), etc. These markers can be quantitatively detected by ELISA and other methods, and the enzyme-labeled plate is the main tool for ELISA experiments;

[0003] The specific operation process of the ELISA plate is as follows:

[0004] Coating: Fixing specific antibodies in the wells of an ELISA plate or strip to capture the corresponding antigens in the sample to be tested.

[0005] Blocking: Fill each well with a blocking solution (such as bovine serum albumin or nonfat dry milk) to reduce nonspecific binding.

[0006] Sample addition: Add the sample or standard to be tested into the wells of the ELISA plate to allow the antigen to bind to the coated antibody.

[0007] Wash: Wash each well with wash buffer to remove unbound sample.

[0008] Enzyme-labeled antibody incubation: Add enzyme-labeled secondary antibody to bind to the bound antigen-antibody complex.

[0009] Wash: Wash again to remove unbound enzyme-labeled antibody.

[0010] Substrate color development: When the substrate of the enzyme is added, such as tetramethylbenzidine (TMB), the color changes under the action of the enzyme.

[0011] Termination reaction: Add stop solution (such as sulfuric acid) to stop the enzyme reaction.

[0012] Determination: Use an enzyme-labeled instrument to measure the absorbance (OD value) of each well at a specific wavelength, and calculate the concentration of the analyte in the sample based on the standard curve.

[0013] In actual operation, enzyme label strips are generally used for detecting a small amount of markers, such as Figure 6The shape shown is a strip shape composed of multiple short tubes connected in series; in order to ensure that the enzyme labeling strip can be stable and not tip over when in use, the enzyme labeling strip is generally placed in a special tray, and the tray is provided with a placement groove adapted to each tube; when the existing tray is used in conjunction with the enzyme labeling strip, the enzyme labeling strip is placed in the placement tray, and each tube of the enzyme labeling strip is placed in the placement groove. This process can be directly placed with one hand, and after the enzyme labeling strip is taken out of the tray, one hand holds the liquid gun, and the other hand holds the end of the enzyme labeling strip. At this time, the entire enzyme labeling strip moves up from the tray in an inclined state, and when moving up and out of the placement groove, the lower end edge of each tube will be squeezed on the inner side wall of the placement groove. At this time, the upward movement of the enzyme labeling strip will drive the tray to move, making it difficult to smoothly take out the enzyme labeling strip.

[0014] Therefore, in order to solve the above problems, a colorectal cancer tumor marker detection device is proposed. Summary of the invention

[0015] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0016] The technical solution adopted by the present invention to solve the technical problem is as follows: a colorectal cancer tumor marker detection device according to the present invention comprises a tray box, wherein the tray box comprises a bottom plate and a plurality of transverse plates and longitudinal plates arranged on the bottom plate, wherein the transverse plates and longitudinal plates are vertically staggered to form a plurality of rows of placement slots, and enzyme label strips are placed in the placement slots;

[0017] A sliding hole is formed at the end of each of the transverse plates, and a sliding groove is formed at a position opposite to the sliding hole and each placement groove, and the sliding groove is connected to the sliding hole and the placement groove;

[0018] A push rod is arranged in each of the sliding holes, the end of the push rod passes through the outside of the sliding hole, and a limiting structure is arranged on the push rod at a position relative to the sliding groove;

[0019] Each of the limiting structures includes a connecting part and an extruding part. One end of the connecting part extends along the slide groove into the slide hole and is connected to the push rod. The other end of the connecting part is fixedly connected to the extruding part, and the extruding part extends upward to the notch position of the placement groove.

[0020] Preferably, a plurality of sleeves are provided on one side of the upper surface of the bottom plate, each sleeve is opposite to one end of the sliding hole, and one end of the push rod extends into the sleeve and is connected to the sleeve through a spring;

[0021] A plurality of recesses are provided on the other side of the upper surface of the bottom plate, each recess is arranged opposite to the other end hole of the sliding hole, and an elastic plate is provided in the recess, and a plurality of teeth are provided on the upper surface of the elastic plate; a push plate is provided at the other end of each push rod, and a clamping block is provided on the lower edge of the push plate, and the clamping block can be clamped on the clamping teeth on the upper surface of the elastic plate.

[0022] Preferably, each of the push rods is radially provided with a plurality of annular clamping grooves, and each clamping groove is arranged opposite to the placement groove one by one;

[0023] One end of each connecting portion is arranged in a C shape, and one end of the connecting portion is half-wrapped and can be clamped in the clamping groove.

[0024] Preferably, the other end of each of the connecting parts is fixedly connected to the extrusion part through a rubber block, and each extrusion part is arranged in an arc shape, and the arc surface of the extrusion part can be extruded on the outer side wall of the enzyme label strip.

[0025] Preferably, storage grooves are provided on the upper surfaces at both ends of the base plate, support rods are provided in the storage grooves, one end of the support rods is rotatably connected in the storage grooves, and insertion holes are provided on the lower surfaces at both ends of the base plate, and the support rods can support and separate the upper and lower layers of the base plates.

[0026] Preferably, chamfers are provided on both sides of one end of each of the connecting parts.

[0027] Preferably, a plurality of grooves are arranged in a matrix on the lower surface of the bottom plate, and the grooves are arranged opposite to the placement slots one by one, and a plurality of transparent hemispheres are arranged in each groove, and the spherical surface of the hemisphere is fixed to the top surface position in the groove.

[0028] Preferably, the lower surface of the hemisphere in the center of each groove is horizontally arranged, and the lower surface of the hemisphere outside the center of each groove is inclined.

[0029] Preferably, both ends of each sleeve are open.

[0030] Preferably, the outer edge of each notch is arranged in a flared shape.

[0031] The present invention is beneficial in that:

[0032] 1. In the present invention, a tray is designed to cooperate with the enzyme labeling strip, and a push rod is arranged in the tray. The push rod cooperates with the limiting structure to squeeze the enzyme labeling strip into the placement slot and fix it. At the same time, the placement slot also makes room, which makes it convenient for the enzyme labeling strip to be taken out of the placement slot in an inclined state, and facilitates the detection personnel to operate the detection process.

[0033] 2. In the present invention, the push rod and the limiting structure in the tray are designed to be detachable, which is convenient for cleaning and disinfection of the tray, as well as cleaning and disinfection of the push rod and the limiting structure themselves, ensuring that the tray is thoroughly cleaned to prevent residual bacteria or viruses from affecting subsequent detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 A first-view stereoscopic view of the tray in the present invention;

[0035] Figure 2A second perspective stereoscopic view of the tray in the present invention;

[0036] Figure 3 A top view of the tray in the present invention;

[0037] Figure 4 It is a three-dimensional diagram of the cooperation of the transverse plate and the longitudinal plate in the present invention;

[0038] Figure 5 It is a three-dimensional diagram of the coordination between the elastic plate and the push rod in the present invention;

[0039] Figure 6 It is a stereogram of the enzyme labeling strip in the present invention;

[0040] Figure 7 It is a three-dimensional diagram of the cooperation between the enzyme label strip and the extrusion part in the present invention;

[0041] Figure 8 is a three-dimensional diagram of the push rod in the present invention;

[0042] Fig. 9 It is a three-dimensional diagram of the cooperation between the push rod and the limiting structure in the present invention;

[0043] Fig.10 It is a front view of the limiting structure in the present invention;

[0044] Fig.11 This is a schematic diagram of the installation of the limiting structure in the present invention;

[0045] Fig.12 is a cross-sectional view of the bottom plate of the present invention;

[0046] Fig.13 It is a schematic diagram of the cooperation between the push rod and the sleeve in the present invention;

[0047] Fig.14 It is a schematic diagram of the cooperation between the clamping block and the clamping teeth in the present invention;

[0048] Fig.15 A bottom view of the tray in the present invention;

[0049] Fig.16 It is a schematic diagram of the cooperation between the hemisphere and the placement groove in the present invention.

[0050] In the figure: 1. tray; 2. bottom plate; 3. horizontal plate; 4. longitudinal plate; 5. placement slot; 6. enzyme labeling strip; 7. tube body; 8. sliding hole; 9. sliding slot; 10. push rod; 11. connecting part; 12. extrusion part; 13. sleeve; 14. spring; 15. elastic plate; 16. latch tooth; 17. push plate; 18. clamping block; 19. clamping slot; 20. rubber block; 21. storage slot; 22. support rod; 23. jack; 24. chamfer; 25. groove; 26. hemisphere; 27. notch. DETAILED DESCRIPTION

[0051] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0052] Reference Figure 1 - Figure 7 A colorectal cancer tumor marker detection device comprises a tray 1, wherein the tray 1 comprises a bottom plate 2 and a plurality of transverse plates 3 and longitudinal plates 4 arranged on the bottom plate 2, wherein the transverse plates 3 and longitudinal plates 4 are vertically staggered to form a plurality of rows of placement slots 5, wherein enzyme label strips 6 are placed in the placement slots 5;

[0053] A sliding hole 8 is formed at the end of each of the transverse plates 3, and a sliding groove 9 is formed at a portion of the sliding hole 8 opposite to each placement groove 5, and the sliding groove 9 is connected to the sliding hole 8 and the placement groove 5;

[0054] A push rod 10 is disposed in each of the slide holes 8, the end of the push rod 10 passes through the outside of the slide hole 8, and a limiting structure is disposed on the push rod 10 at a position relative to the slide groove 9;

[0055] Each of the limiting structures includes a connecting portion 11 and an extruding portion 12. One end of the connecting portion 11 extends along the slide groove 9 into the slide hole 8 and is connected to the push rod 10. The other end of the connecting portion 11 is fixedly connected to the extruding portion 12. The extruding portion 12 extends upward to the notch position of the placement groove 5.

[0056] In this embodiment, the colorectal cancer tumor marker detection device also includes an ELISA instrument. The ELISA strip 6 is placed in the tray 1, and then the tray 1 together with the ELISA strip 6 is placed in the ELISA instrument for detection and analysis; when the tray 1 provided in this embodiment is used in conjunction with the ELISA strip 6, the specific process is as follows:

[0057] Place the enzyme label strip 6, first pull the push rod 10, and pull the push rod 10 to the right side of the bottom plate 2, such as Figure 3 As shown, the push rod 10 drives the extrusion part 12 connected thereto to move synchronously to the right side of the bottom plate 2, and the empty space in the placement groove 5 is vacated, and then the enzyme labeling strip 6 is placed, and each tube body 7 of the enzyme labeling strip 6 is located in the placement groove 5, and then the push rod 10 is pushed to the left side of the bottom plate 2. At this time, the push rod 10 drives the extrusion part 12 thereon to squeeze on the outer side wall of each tube body 7 of the enzyme labeling strip 6. At this time, the extrusion part 12 squeezes and limits the entire enzyme labeling strip 6, and the enzyme labeling strip 6 is stabilized;

[0058] To remove the enzyme label strip 6, the operation is opposite to that of placing the enzyme label strip 6. The push rod 10 is pulled to move to the right side of the bottom plate 2. At this time, the squeezing portion 12 no longer squeezes each tube body 7 of the enzyme label strip 6, and space is vacated in the placement groove 5. At this time, the space in each placement groove 5 is enough to allow the tube body 7 to move up smoothly from the placement groove 5 in an inclined state, that is, the inner side wall of the placement groove 5 and the side wall of the squeezing portion 12 will not hinder the upward movement and separation of the tube body 7. Even if the entire enzyme label strip 6 moves up from the tray 1 in an inclined state, it can also be separated smoothly. For the detection personnel, one hand can hold the liquid extraction gun, and the other hand can smoothly take the enzyme label strip 6 out of the tray 1, which is convenient for the detection personnel to operate.

[0059] Reference Figure 1 - Figure 7 ,as well as Fig.12 - Fig.14 A plurality of sleeves 13 are provided on one side of the upper surface of the bottom plate 2, each sleeve 13 is opposite to one end of the sliding hole 8, and one end of the push rod 10 extends into the sleeve 13 and is connected to the sleeve 13 through a spring 14;

[0060] A plurality of notches 27 are provided on the other side of the upper surface of the bottom plate 2, each notch 27 is arranged opposite to the other end hole of the slide hole 8, and an elastic plate 15 is arranged in the notch 27, and a plurality of latching teeth 16 are arranged on the upper surface of the elastic plate 15; a push plate 17 is provided at the other end of each push rod 10, and a latching block 18 is provided at the lower edge of the push plate 17, and the latching block 18 can be latched on the latching teeth 16 on the upper surface of the elastic plate 15;

[0061] When placing the enzyme label strip 6, the enzyme label strip 6 is located in the placement groove 5, and then the push plate 17 is set at the other end of the push rod 10, and the push plate 17 drives the push rod 10 to move to the left direction of the bottom plate 2. At the same time, the block 18 set at the lower edge of the push plate 17 moves along the elastic plate 15. When the push rod 10 moves into place, the block 18 is stuck on the tooth 16. At the same time, one end of the push rod 10 is squeezed in the sleeve 13 by the spring 14. When the enzyme label strip 6 needs to be taken out of the tray 1, the inspector presses the elastic plate 15 with his finger, and the elastic plate 15 tilts downward. At the same time, the tooth 16 on the elastic plate 15 will also be separated from the block 18. Under the elastic force of the spring 14 connected to it, the push rod 10 moves to the right direction of the bottom plate 2 and drives the extrusion part 12 to move. The extrusion part 12 no longer squeezes the enzyme label strip 6, which further facilitates the inspection personnel to operate the inspection process.

[0062] Reference Figure 1 - Fig.14 A plurality of annular clamping grooves 19 are radially provided on each of the push rods 10, and each clamping groove 19 is arranged opposite to the placement groove 5 one by one;

[0063] One end of each of the connecting parts 11 is arranged in a C shape, and one end of the connecting part 11 is half-wrapped and can be clamped in the clamping groove 19;

[0064] Considering the cleaning of the tray 1 and the convenience of recycling, after multiple uses, it is necessary to perform operations such as cleaning and sterilization. When cleaning, it is necessary to clean every part of the tray 1. For this purpose, the push rod 10 and the limiting structure are designed to be detachable, that is, one end of the connecting portion 11 is in a C shape, which can extend along the slide groove 9 to the slide hole 8 and be stuck in the clamping groove 19 on the push rod 10. The limiting structure is dragged with force to make the connecting portion 11 detach from the push rod 10, and then the push rod 10 is taken out of the slide hole 8. Each placement groove 5, slide groove 9 and slide hole 8 is immersed in cleaning and disinfection. At the same time, the removed slide rod and limiting structure are also fully cleaned and disinfected to ensure that the tray 1 is thoroughly cleaned to prevent residual bacteria or viruses from affecting subsequent detection;

[0065] After cleaning, disinfection and drying, assembly is performed. First, each push rod 10 is installed in the slide hole 8, and then the limiting structure is installed. The C-shaped part of the connecting part 11 is aligned with the slide groove 9, and it is squeezed hard into the clamping groove 19 on the push rod 10.

[0066] Reference Fig.10 The other end of each of the connecting parts 11 is fixedly connected to the extrusion part 12 through a rubber block 20, and each extrusion part 12 is arranged in an arc shape, and the arc surface of the extrusion part 12 can be extruded on the outer side wall of the enzyme labeling strip 6;

[0067] Taking into account that the sizes of the enzyme labeling strip 6 are various, that is, the diameters of the tube body 7 of the enzyme labeling strip 6 are of different specifications, and there are situations where enzyme labeling strips 6 of different specifications are placed in the same row of placement grooves 5, a rubber block 20 is provided at the connecting portion 11 and the extrusion portion 12. By utilizing the elasticity of the rubber block 20, the extrusion portions 12 of the same row can effectively squeeze tube bodies 7 of different specifications, thereby achieving the extrusion and fixation of the enzyme labeling strips 6 with tube bodies 7 of different specifications.

[0068] Reference Figure 1 - Figure 3 ,as well as Fig.12 The upper surfaces of both ends of the bottom plate 2 are provided with receiving grooves 21, and the receiving grooves 21 are provided with support rods 22, one end of the support rods 22 is rotatably connected in the receiving grooves 21, and the lower surfaces of both ends of the bottom plate 2 are provided with insertion holes 23, and the support rods 22 can support and separate the upper and lower layers of the bottom plates 2;

[0069] The support rod 22 is provided to separate the upper and lower layers of the tray 1. The support rod 22 is rotated perpendicular to the base plate 2, and the upper end of the support rod 22 is inserted into the socket 23 on the lower surface of the upper tray 1, so as to facilitate the detection and stacking of a large number of markers. When support is not needed, the support rod 22 can be rotated into the storage slot 21 to prevent the support rod 22 from obstructing the detection operation.

[0070] Reference Fig. 9 - Fig.11 , chamfers 24 are provided on both sides of one end of each of the connecting portions 11;

[0071] Chamfers 24 are provided on both sides of one end of the connecting portion 11. Fig.11 As shown, when installing the limiting structure, the limiting structure is placed in an inclined state so that one end of the connecting portion 11 is quickly aligned with the snap-in groove 19, and then the connecting portion 11 is squeezed hard, and the limiting structure and the push rod 10 are rotated into a vertical state, which facilitates the installation connection between each limiting structure and the push rod 10, and there is no need to install the limiting structure by brute force.

[0072] Reference Fig.15 - Fig.16 The bottom surface of the bottom plate 2 is provided with a plurality of grooves 25 in a matrix, and the grooves 25 are arranged opposite to the placement slots 5 one by one, and each groove 25 is provided with a plurality of transparent hemispherical bodies 26, and the spherical surface of the hemispherical body 26 is fixed to the top surface of the groove 25;

[0073] The groove 25 is used to protect the hemisphere 26, and the hemisphere 26 is used to focus the light beam emitted by the microplate reader so that the light beam can be concentrated on the tube body 7 in the placement groove 5, thereby improving the utilization effect of light; and the light beam emitted by the light source lamp of the microplate reader becomes a beam of monochromatic light after passing through the filter. The monochromatic light beam is focused by the hemisphere 26 and irradiated onto the sample to be tested in the tube body 7. After a part of it is absorbed, it reaches the photoelectric detector. The photoelectric detector converts the intensity of the light signal projected onto it into the size of the electrical signal. After the electrical signal is processed by analog signal such as analog-to-digital conversion, it is sent to the microprocessor for data processing and calculation, and finally the result output part outputs the test result.

[0074] Reference Fig.16 The lower surface of the hemispherical body 26 in the center of each groove 25 is horizontally arranged, and the lower surface of the hemispherical body 26 outside the center of each groove 25 is inclined;

[0075] The inclined arrangement of the hemispherical body 26 at the center periphery of the groove 25 enables the light beam scattered outwards by the light source lamp to be refracted to the surface of the tube body 7, further improving the utilization rate of the light beam.

[0076] Reference Fig.13 Both ends of each sleeve 13 are open; both ends of the sleeve 13 are open, so that during subsequent cleaning, it is convenient for air to flow along the sleeve 13, which helps to thoroughly dry the tray 1.

[0077] Reference Figure 5The outer edge of each of the notches 27 is flared; the flared setting of the notches 27 facilitates the inspection personnel to press the elastic plate 15 with their fingers.

[0078] Working principle: In this embodiment, the colorectal cancer tumor marker detection device also includes an ELISA instrument. The ELISA strip 6 is placed in the tray 1, and then the tray 1 together with the ELISA strip 6 is placed in the ELISA instrument for detection and analysis; when the tray 1 set in this embodiment is used in conjunction with the ELISA strip 6, the specific process is as follows:

[0079] Place the enzyme label strip 6, first pull the push rod 10, and pull the push rod 10 to the right side of the bottom plate 2, such as Figure 3 As shown, the push rod 10 drives the extrusion part 12 connected thereto to move synchronously to the right side of the bottom plate 2, and the empty space in the placement groove 5 is vacated, and then the enzyme labeling strip 6 is placed, and each tube body 7 of the enzyme labeling strip 6 is located in the placement groove 5, and then the push rod 10 is pushed to the left side of the bottom plate 2. At this time, the push rod 10 drives the extrusion part 12 thereon to squeeze on the outer side wall of each tube body 7 of the enzyme labeling strip 6. At this time, the extrusion part 12 squeezes and limits the entire enzyme labeling strip 6, and the enzyme labeling strip 6 is stabilized;

[0080] To remove the enzyme label strip 6, the operation is opposite to that of placing the enzyme label strip 6. The push rod 10 is pulled to move to the right side of the bottom plate 2. At this time, the squeezing portion 12 no longer squeezes each tube body 7 of the enzyme label strip 6, and space is freed in the placement groove 5. At this time, the space of each placement groove 5 is enough to allow the tube body 7 to smoothly move up from the placement groove 5 in an inclined state, that is, the inner side wall of the placement groove 5 and the side wall of the squeezing portion 12 will not hinder the upward movement and separation of the tube body 7. Even if the entire enzyme label strip 6 moves up from the tray 1 in an inclined state, it can also be smoothly separated. For the tester, one hand can hold the liquid gun, and the other hand can smoothly take the enzyme label strip 6 out of the tray 1, which is convenient for the tester to operate;

[0081] When the enzyme label strip 6 is placed, the enzyme label strip 6 is located in the placement groove 5, and then the push plate 17 is set at the other end of the push rod 10, and the push plate 17 drives the push rod 10 to move to the left direction of the bottom plate 2, and at the same time, the block 18 set at the lower edge of the push plate 17 moves along the elastic plate 15. When the push rod 10 moves into place, the block 18 is stuck on the tooth 16, and at the same time, one end of the push rod 10 is squeezed into the sleeve 13 by the spring 14. When the enzyme label strip 6 needs to be taken out of the tray 1, the inspector presses the elastic plate 15 with his finger, and the elastic plate 15 tilts downward. At the same time, the tooth 16 on the elastic plate 15 will also be separated from the block 18. Under the elastic force of the spring 14 connected to it, the push rod 10 moves to the right direction of the bottom plate 2 and drives the extrusion part 12 to move. The extrusion part 12 no longer squeezes the enzyme label strip 6, which further facilitates the inspection personnel to operate the inspection process;

[0082] Considering the cleaning of the tray 1 and the convenience of recycling, after multiple uses, it is necessary to perform operations such as cleaning and sterilization. When cleaning, it is necessary to clean every part of the tray 1. For this purpose, the push rod 10 and the limiting structure are designed to be detachable, that is, one end of the connecting portion 11 is in a C shape, which can extend along the slide groove 9 to the slide hole 8 and be stuck in the clamping groove 19 on the push rod 10. The limiting structure is dragged with force to make the connecting portion 11 detach from the push rod 10, and then the push rod 10 is taken out of the slide hole 8. Each placement groove 5, slide groove 9 and slide hole 8 is immersed in cleaning and disinfection. At the same time, the removed slide rod and limiting structure are also fully cleaned and disinfected to ensure that the tray 1 is thoroughly cleaned to prevent residual bacteria or viruses from affecting subsequent detection;

[0083] After cleaning, disinfection and drying, assemble. First, install each push rod 10 into the slide hole 8, then install the limiting structure, align the C-shaped part of the connecting part 11 with the slide groove 9, and squeeze it into the clamping groove 19 on the push rod 10.

[0084] Considering that the sizes of the enzyme labeling strip 6 are various, that is, the diameters of the tube body 7 of the enzyme labeling strip 6 are of different specifications, and there are enzyme labeling strips 6 of different specifications placed in the same row of placement slots 5, a rubber block 20 is provided at the connecting portion 11 and the extrusion portion 12. By utilizing the elasticity of the rubber block 20, the extrusion portions 12 of the same row can effectively squeeze the tube bodies 7 of different specifications, thereby achieving the extrusion and fixation of the enzyme labeling strips 6 of the tube bodies 7 of different specifications;

[0085] The support rod 22 is used to separate the upper and lower trays 1. The support rod 22 is rotated perpendicular to the bottom plate 2, and the upper end of the support rod 22 is inserted into the insertion hole 23 on the lower surface of the upper tray 1, which is convenient for the detection and stacking of a large number of markers. When the support is not needed, the support rod 22 can be rotated into the storage slot 21 to avoid the support rod 22 hindering the detection operation.

[0086] Chamfers 24 are provided on both sides of one end of the connecting portion 11. Fig.11 As shown, when installing the limiting structure, the limiting structure is placed in an inclined state so that one end of the connecting portion 11 is quickly aligned with the clamping groove 19, and then the connecting portion 11 is pressed hard, and the limiting structure and the push rod 10 are rotated to a vertical state, which facilitates the installation and connection of each limiting structure and the push rod 10, and does not require brute force to install the limiting structure;

[0087] The groove 25 is used to protect the hemisphere 26, and the hemisphere 26 is used to focus the light beam emitted by the microplate reader, so that the light beam can be concentrated on the tube body 7 in the placement groove 5, thereby improving the utilization effect of light; and the light beam emitted by the light source lamp of the microplate reader becomes a beam of monochromatic light after passing through the filter, and the monochromatic light beam is focused by the hemisphere 26 and irradiated to the sample to be tested in the tube body 7, and after a part of it is absorbed, it reaches the photoelectric detector, and the photoelectric detector converts the intensity of the light signal projected thereon into the size of the electrical signal, and after the electrical signal is processed by analog signal processing such as analog-to-digital conversion, it is sent to the microprocessor for data processing and calculation, and finally the result output part outputs the test result in the setting;

[0088] The inclined arrangement of the hemispherical body 26 at the center periphery of the groove 25 enables the light beam scattered outwards by the light source lamp to be refracted to the surface of the tube body 7, further improving the utilization rate of the light beam.

[0089] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A colorectal cancer tumor marker detection device, characterized in that: The tray box (1) comprises a bottom plate (2) and a plurality of transverse plates (3) and longitudinal plates (4) arranged on the bottom plate (2), wherein the transverse plates (3) and longitudinal plates (4) are vertically staggered to form a plurality of rows of placement grooves (5), and enzyme label strips (6) are placed in the placement grooves (5); A sliding hole (8) is provided at the end of each of the transverse plates (3), a sliding groove (9) is provided at a position of the sliding hole (8) opposite to each placement groove (5), and the sliding groove (9) is connected to the sliding hole (8) and the placement groove (5); A push rod (10) is provided in each of the slide holes (8), the end of the push rod (10) passes through the outside of the slide hole (8), and a limiting structure is provided on the push rod (10) at a position relative to the slide groove (9); Each of the limiting structures comprises a connecting portion (11) and an extruding portion (12); one end of the connecting portion (11) extends along the slide groove (9) into the slide hole (8) and is connected to the push rod (10); the other end of the connecting portion (11) is fixedly connected to the extruding portion (12); and the extruding portion (12) extends upward to the notch position of the placement groove (5).

2. A colorectal cancer tumor marker detection device according to claim 1, characterized in that: A plurality of sleeves (13) are provided on one side of the upper surface of the bottom plate (2), each sleeve (13) is opposite to one end of the sliding hole (8), and one end of the push rod (10) extends into the sleeve (13) and is connected to the sleeve (13) through a spring (14); A plurality of recesses (27) are provided on the other side of the upper surface of the bottom plate (2), each recess (27) being arranged opposite to the other end hole of the sliding hole (8), and an elastic plate (15) is provided in the recess (27), and a plurality of latching teeth (16) are provided on the upper surface of the elastic plate (15); a push plate (17) is provided at the other end of each push rod (10), and a latching block (18) is provided at the lower edge of the push plate (17), and the latching block (18) can be latched on the latching teeth (16) on the upper surface of the elastic plate (15).

3. A colorectal cancer tumor marker detection device according to claim 2, characterized in that: A plurality of annular clamping grooves (19) are radially formed on each push rod (10), and each clamping groove (19) is arranged opposite to the placement groove (5) one by one; One end of each connecting portion (11) is arranged in a C shape, and one end of the connecting portion (11) is half-wrapped and can be clamped in the clamping groove (19).

4. A colorectal cancer tumor marker detection device according to claim 3, characterized in that: The other end of each connecting portion (11) is fixedly connected to the extrusion portion (12) via a rubber block (20), and each extrusion portion (12) is arranged in an arc shape, and the arc surface of the extrusion portion (12) can be extruded on the outer side wall of the enzyme labeling strip (6).

5. A colorectal cancer tumor marker detection device according to claim 2, characterized in that: The upper surfaces of both ends of the bottom plate (2) are provided with receiving grooves (21), and a support rod (22) is provided in the receiving groove (21). One end of the support rod (22) is rotatably connected in the receiving groove (21). The lower surfaces of both ends of the bottom plate (2) are provided with insertion holes (23), and the support rod (22) can support and separate the upper and lower layers of the bottom plates (2).

6. A colorectal cancer tumor marker detection device according to claim 4, characterized in that: Chamfers (24) are provided on both sides of one end of each connecting portion (11).

7. A colorectal cancer tumor marker detection device according to claim 5, characterized in that: The bottom surface of the bottom plate (2) is provided with a plurality of grooves (25) in a matrix, the grooves (25) and the placement slots (5) are arranged one by one in a relative position, and each groove (25) is provided with a plurality of transparent hemispherical bodies (26), the spherical surface of the hemispherical body (26) being fixedly connected to the top surface of the groove (25).

8. A colorectal cancer tumor marker detection device according to claim 7, characterized in that: The lower surface of the hemispherical body (26) in the center of each groove (25) is arranged horizontally, and the lower surface of the hemispherical body (26) outside the center of each groove (25) is arranged inclined.

9. A colorectal cancer tumor marker detection device according to claim 2, characterized in that: Both ends of each sleeve (13) are open.

10. A colorectal cancer tumor marker detection device according to claim 2, characterized in that: The outer edge of each notch (27) is arranged in a flared shape.

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