Vaginal lactobacillus and application thereof

Abstract

The invention discloses vaginal lactobacillus and application thereof, and belongs to the technical field of microorganisms. The invention relates to vaginal lactobacillus which is deposited in the China General Microbiological Culture Collection Center, and particularly comprises lactobacillus jensenii. The preservation number of the lactobacillus jensenii is CGMCC (China General Microbiological Culture Collection Center) NO.22802; the vaginal lactobacillus provided by the invention can remarkably inhibit proliferation and migration of cervical cancer cells in vitro, and has a remarkable effect in the aspects of regulation of vaginal flora disorder and prevention and treatment of cervical cancer.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, in particular to vaginal lactobacillus and application thereof. Background Art

[0002] Cervical cancer is the fourth most common malignant tumor in women, with 570,000 new cases and 311,000 deaths each year. Cervical cancer has become a public health issue that seriously affects the health of women worldwide. While there is no clear conclusion on the cause of cervical cancer, relevant studies have shown that genetics, environment, smoking, contraception, sexual behavior, bacteria, viruses and other factors may be related to the occurrence and development of cervical cancer.

[0003] In recent years, the research on the mechanism of cervical cancer development has been continuously deepened, including vaginal flora disorder; local pH increase; HPV infection; increased immune inflammatory factors, etc. These factors work together to promote the transformation of normal cells into cancer cells. Although the understanding of its pathogenesis continues to deepen, the means of prevention and treatment of cervical cancer are still very limited. The prevention and treatment methods include chemotherapy, radiotherapy, immunotherapy and other methods, all of which have certain limitations and the results are often unsatisfactory. Therefore, new prevention and treatment methods are urgently needed.

[0004] Lactobacillus spp. is the dominant bacteria in the vaginal microenvironment of healthy women, and has a mutually beneficial relationship with the host. On the one hand, it inhibits the invasion of pathogenic microorganisms by blocking the adhesion of pathogenic microorganisms and producing antibacterial substances to maintain the homeostasis of the vaginal microenvironment. On the other hand, it uses the glycogen of the host vaginal mucosal epithelial cells as energy to promote its own growth and reproduction. With the development of isolation culture and high-throughput sequencing technology, current studies have found that Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus iners, and Lactobacillus jensenii are the most common Lactobacillus species in the vaginal microenvironment of healthy women. Under physiological conditions, lactobacillus produces lactic acid, which promotes the pH of the vaginal microenvironment to ≤4.5, which is a sign of the homeostasis of the vaginal microenvironment. The abundance of Lactobacillus in the vagina of patients with cervical cancer decreased significantly, while the abundance of multiple opportunistic pathogens increased significantly. Therefore, vaginal Lactobacillus is of great significance in the prevention and treatment of cervical cancer. However, there are currently few articles reporting the role of vaginal Lactobacillus in the prevention and treatment of cervical cancer in healthy people. Summary of the invention

[0005] 1. Technical Problems to be Solved by the Present Invention

[0006] The purpose of the present invention is to provide a vaginal lactobacillus which can significantly inhibit the proliferation and migration of cervical cancer cells in vitro and play a significant role in regulating vaginal flora disorders and preventing and treating cervical cancer.

[0007] 2. Technical solution

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A vaginal lactobacillus, deposited in the General Microbiology Center of China Microbiological Culture Collection Administration, specifically including a Lactobacillus jensenii;

[0010] The Lactobacillus jensenii has a preservation number of CGMCC NO.22802.

[0011] Preferably, the Lactobacillus jensenii is isolated from the vagina of a healthy woman.

[0012] Preferably, the 16S rDNA sequence of the Lactobacillus jensenii with the accession number of CGMCC NO.22802 is: CCATTAGGCGGCTGGCTCCAAAGGTTACCTCACCGACTTTGGGTGTTACAAACTCTCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCAGCTTCGTGTAGGCGAGTTGCAGCCTACAGTCCGAACTGAGAACAGCTTTAAGAGATCCGCTTGCCTTCACAGGTTCGCTTCTCGTTGTACTGCCCATTGTAGCACGTGTGTAGCCCAGGTCA

[0013] TAAGGGGCATGATGACTTGACGTCATCCCCACCTTCCTCCGGT

[0014] TTGTCACCGGCAGTCTCAATAGAGTGCCCAACTTAATGCTGGC

[0015] AACTATTAACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAAC

[0016] ATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTCTC

[0017] TTTGTCCCCGAAGGGAAAACCTAATCTCTTAGGTGGTCAAAGG

[0018] ATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAA

[0019] CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTG

[0020] AGTTTCAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAAT

[0021] GCGTTAGCTGCAGCACTGAGAGGCGGAAACCTCCCAACACTT

[0022] AGCACTCATCGTTTACGGCATGGACTACCAGGGTATCTAATCC

[0023] TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGAC

[0024] CAGAGAGCCGCCTTCGCCACTGGTGTTCTTCCATATATCTACG

[0025] CATTCCACCGCTACACATGGAGTTCCACTCTCCTCTTCTGCACT

[0026] CAAGATTGACAGTTTCTGATGCAGTTCTTCGGTTGAGCCGAAG

[0027] GCTTTCACATCAGACTTATCAATCCGCCTGCGCTCGCTTTACGC

[0028] CCAATAAATCCGGACAACGCTTGCCACCTACGTATTACCGCGG

[0029] CTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGATTACCGTC

[0030] AAATAAAGGCCAGTTACTACCTCTATCCTTCTTCACCAACAAC

[0031] AGAGCTTTACGATCCGAAAACCTTCTTCACTCACGCGGCGTTG

[0032] CTCCATCAGACTTGCGTCCATTGTGGAAGATTCCCTACTGCTG

[0033] CCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCCAATGTGGCC

[0034] GATCAGTCTCTCAACTCGGCTATGCATCATCGCCTTGGTAAGC

[0035] CGTTACCTTACCAACTAGCTAATGCATCGCAGGTCCATCCTTT

[0036] AGCGACAGCTTACGCCGCCTTTTAAACTTCTTTCATGCGAAAG

[0037] TAGCTTTTTATCCGGTATTAGCATCTGTTTCCAAATGGTATCCCA

[0038] GACTTAAGGGCAGGTTACCCACGCGTTACTCACCCATCCGCCG

[0039] CTAGCTTGTATCTATGTCCATTCCGAAGAATTTCAATAGGCAA

[0040] GCTCGCTCGACTTGCATGTATTAGGCACGCCGCCAGCGTTCGTCCTGAGCCAGATTCCAAAC.

[0041] The invention discloses an application of vaginal lactobacillus, wherein the lactobacillus jensenii can significantly inhibit the proliferation and migration of cervical cancer cells in vitro, and has important applications in the regulation of vaginal flora disorder and the prevention and treatment of cervical cancer.

[0042] 3. Beneficial effects

[0043] The present invention proposes that a lactobacillus isolated from the human body can significantly inhibit the proliferation and migration of cervical cancer cells in vitro, which will have important application prospects in the regulation of vaginal flora disorders and the prevention and treatment of cervical cancer. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is a diagram showing the analysis of the vaginal flora structure of healthy volunteers and cervical cancer patients using the two vaginal lactobacilli proposed in Example 2 of the present invention;

[0045] Figure 2A schematic diagram of colonies of two vaginal lactobacilli and their application, Lactobacillus crispatus and Lactobacillus jannaschii, proposed in Example 3 of the present invention;

[0046] Figure 3 A schematic diagram of Gram staining of two vaginal lactobacilli and their separated Lactobacillus crispatus and Lactobacillus jannaschii proposed in Example 3 of the present invention;

[0047] Figure 4 It is a schematic diagram of the whole genome map and comparison result of the two vaginal lactobacilli and their application, Lactobacillus crispatus and Lactobacillus jannaschii, proposed in Example 3 of the present invention;

[0048] Figure 5 This is a schematic diagram of the effects of the two vaginal lactobacilli proposed in Example 4 of the present invention, and the separated Lactobacillus crispatus and Lactobacillus jensenii used therein, on the proliferation of cervical cancer cells in vitro;

[0049] Figure 6 This is a schematic diagram of the effects of the two vaginal lactobacilli and their applied isolated Lactobacillus crispatus and Lactobacillus jensenii on the migration of cervical cancer cells in vitro in Example 5 of the present invention. DETAILED DESCRIPTION

[0050] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0051] Embodiment 1:

[0052] A vaginal lactobacillus, deposited in the General Microbiology Center of China Microbiological Culture Collection Administration, specifically including a Lactobacillus jensenii;

[0053] Lactobacillus jensenii, its preservation number is CGMCC NO.22802.

[0054] Lactobacillus jensenii was isolated from the vagina of healthy women.

[0055] Lactobacillus jensenii with the preservation number of CGMCC NO.22802, its 16S rDNA sequence is: CCATTAGGCGGCTGGCTCCAAAGGTTACCTCACCGACTTTGGGTGTTACAAACTCTCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCAGCTTCGTGTAGGCGAGTTGCAGCCTACAGTCCGAACTGAGAACAGCTTTAAGAGATCCGCTTGCCTTCACAGGTTCGCTTCTCGTTGTACTGCCCATTGTAGCACGTGTGTAGCCCAGGTCA

[0056] TAAGGGGCATGATGACTTGACGTCATCCCCACCTTCCTCCGGT

[0057] TTGTCACCGGCAGTCTCAATAGAGTGCCCAACTTAATGCTGGC

[0058] AACTATTAACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAAC

[0059] ATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTCTC

[0060] TTTGTCCCCGAAGGGAAAACCTAATCTCTTAGGTGGTCAAAGG

[0061] ATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAA

[0062] CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTG

[0063] AGTTTCAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAAT

[0064] GCGTTAGCTGCAGCACTGAGAGGCGGAAACCTCCCAACACTT

[0065] AGCACTCATCGTTTACGGCATGGACTACCAGGGTATCTAATCC

[0066] TGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGAC

[0067] CAGAGAGCCGCCTTCGCCACTGGTGTTCTTCCATATATCTACG

[0068] CATTCCACCGCTACACATGGAGTTCCACTCTCCTCTTCTGCACT

[0069] CAAGATTGACAGTTTCTGATGCAGTTCTTCGGTTGAGCCGAAG

[0070] GCTTTCACATCAGACTTATCAATCCGCCTGCGCTCGCTTTACGC

[0071] CCAATAAATCCGGACAACGCTTGCCACCTACGTATTACCGCGG

[0072] CTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGATTACCGTC

[0073] AAATAAAGGCCAGTTACTACCTCTATCCTTCTTCACCAACAAC

[0074] AGAGCTTTACGATCCGAAAACCTTCTTCACTCACGCGGCGTTG

[0075] CTCCATCAGACTTGCGTCCATTGTGGAAGATTCCCTACTGCTG

[0076] CCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGTGGCC

[0077] GATCAGTCTCTCAACTCGGCTATGCATCATCGCCTTGGTAAGC

[0078] CGTTACCTTACCAACTAGCTAATGCATCGCAGGTCCATCCTTT

[0079] AGCGACAGCTTACGCCGCCTTTTAAACTTCTTTCATGCGAAAG

[0080] TAGCTTTTTATCCGGTATTAGCATCTGTTTCCAAATGGTATCCCA

[0081] GACTTAAGGGCAGGTTACCCACGCGTTACTCACCCATCCGCCG

[0082] CTAGCTTGTATCTATGTCCATTCCGAAGAATTTCAATAGGCAA

[0083] GCTCGCTCGACTTGCATGTATTAGGCACGCCGCCAGCGTTCGTCCTGAGCCAGATTCCAAAC.

[0084] An application of vaginal lactobacillus, Lactobacillus jensenii can significantly inhibit the proliferation and migration of cervical cancer cells in vitro, and has important applications in the regulation of vaginal flora disorders and the prevention and treatment of cervical cancer.

[0085] Embodiment 2:

[0086] See also Figure 1 , combined with the basis of Example 1, the difference is that,

[0087] Comparison of vaginal flora composition between healthy volunteers and patients with cervical cancer

[0088] 1) DNA extraction: Vaginal swab specimens from healthy volunteers and cervical cancer patients were taken out of a -80°C freezer and thawed on ice. After returning to room temperature, DNA from each vaginal swab was extracted according to the QlAamp DNA mini Kit operating instructions. The obtained DNA samples were tested for concentration (ng / μL) and purity (A260 / A280) using a Nano Drop 2000 (Thermo) instrument. The A260 / A280 ratio should generally be between 1.8 and 2.0. The extracted DNA was then packaged and stored;

[0089] 2) High-throughput sequencing analysis of vaginal flora 16S rRNA gene: Illuminate Hiseq PE250 16SrRNA gene sequencing analysis technology was used to sequence and analyze the extracted vaginal swab DNA:

[0090] A1. First, after quality control analysis, the DNA samples were uploaded to QIIME (Quantitative Insights IntoMicrobial Ecology, v1.8.0) for further research;

[0091] A2. Then perform OTUs (Operational Taxonomic Units) clustering and species classification analysis based on valid data;

[0092] A3. Based on the OTUs clustering results, species annotation was performed on the representative sequences of each OUT. The vaginal flora composition of healthy volunteers and cervical cancer patients was as follows: Figure 1 As shown in the results, the vaginal flora of patients with cervical cancer was significantly disturbed, and the abundance of vaginal lactobacilli was significantly reduced.

[0093] Embodiment 3:

[0094] See also Figure 2-4 , based on Example 1-2 but with the difference that,

[0095] Preparation of culture medium

[0096] Preparation of separation culture medium: The culture medium used for the separation and culture of Lactobacillus crispatus and Lactobacillus jensenii is MRS solid culture medium. The mass concentration of each component of MRS solid culture medium is as follows: peptone 10g, beef extract powder 8g, yeast extract powder 4g, glucose 20g, potassium dihydrogen phosphate 2g, diammonium hydrogen citrate 2g, sodium acetate 5g, magnesium sulfate 0.2g, manganese sulfate 0.04g, agar 14g, Tween 80 1g. The pH value is adjusted to 6.5±0.2 at room temperature and dissolved in 1L distilled water.

[0097] Preparation of culture medium for enrichment: The culture medium used for enrichment of Lactobacillus crispatus and Lactobacillus jensenii is MRS broth medium. The mass concentration of each component of MRS broth medium is as follows: 10g peptone, 8g beef extract powder, 4g yeast extract powder, 20g glucose, 2g potassium dihydrogen phosphate, 2g diammonium hydrogen citrate, 5g sodium acetate, 0.2g magnesium sulfate, 0.04g manganese sulfate. The pH value is adjusted to 5.7±0.2 at room temperature and dissolved in 1L distilled water.

[0098] Isolation and identification of Lactobacillus crispatus and Lactobacillus jensenii

[0099] Isolation of bacterial strains

[0100] Dissolve the vaginal swab sample from a healthy person in 1 mL of sterile saline, squeeze it repeatedly until the liquid becomes turbid, and then use a cotton swab to evenly spread it on an MRS solid plate. Incubate it at 37°C in an anaerobic environment for 24 hours, and select obvious light white colonies for isolation, purification, and preservation.

[0101] Identification of bacterial species

[0102] 1) Morphological observation: The isolated strains were inoculated on MRS solid medium and cultured at 37°C in an anaerobic environment for 24 h. The colony morphology was observed. Figure 2As shown, one of the isolated strains has white colonies with irregular edges, flaky shapes, and a convex center; the other strain has round, white colonies with neat edges and smooth colonies;

[0103] 2) Gram staining: Gram stain the two strains and observe them under an optical microscope. Figure 3 As shown, both strains were Gram-positive bacilli;

[0104] 3) Molecular biological identification: 16S rDNA was amplified for the strains obtained by screening, and the purified PCR products were sequenced. The sequencing results are shown in the sequence table in Example 1; the whole genome sequencing of the screened strains was performed, and the whole genome sequencing map and comparison results are shown in Figure 4 shown.

[0105] Based on the results of morphology, Gram staining and molecular biological identification, one of the isolated bacteria was Lactobacillus crispatus and the other was Lactobacillus jensenii.

[0106] The above-isolated Lactobacillus crispatus and Lactobacillus jensenii were deposited in the General Microbiology Center of China Culture Collection Administration (CGMCC for short, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences) in June 2021, and the deposit registration number is CGMCC).

[0107] Embodiment 4:

[0108] See also Figure 5 , based on Examples 1-3 but with the difference that,

[0109] Effects of Lactobacillus crispatus and Lactobacillus jensenii on the proliferation of cervical cancer cells in vitro. The specific experimental steps are as follows:

[0110] 1) Prepare a single cell suspension of cervical cancer cell SiHa in a culture medium containing 5% fetal bovine serum, add 100 μL of 5,000 cells to a 96-well plate, and perform 3 replicate wells for each experimental group or control group;

[0111] 2) Pick the single colonies of Lactobacillus crispatus and Lactobacillus jensenii isolated in Example 2, inoculate them in 100 mL of MRS broth medium, and culture them at 37°C under anaerobic conditions. The bacterial culture solution was aspirated every other day, and the optical density (OD) at 600 nm was measured using an enzyme marker to monitor bacterial growth. When OD600 = 1.5, centrifuge at 5,000 rpm for 10 min, collect the bacteria and culture solution, and add the supernatant to the cervical squamous cell carcinoma cell suspension after filtering through a 0.2 μm filter. MRS medium was used as a control. MOI, that is, the ratio of bacteria to cells, was 1:1000.

[0112] 3) Culture overnight at 37°C and 5% CO2;

[0113] 4) Dilute 5*MTT to 1*MTT, add 50 μL 1*MTT to each well, and incubate at 37°C for 4 h to reduce MTT to formazan;

[0114] 5) Terminate the culture, carefully pour out the supernatant in the wells to avoid cell loss, add 50 μL of dimethyl sulfoxide (DMSO) to each well to fully dissolve the crystals, shake well with a plate shaker, and finally measure the absorbance at a wavelength of 595 nm with an enzyme-labeled instrument.

[0115] Results Figure 5 The isolated Lactobacillus crispatus and Lactobacillus jensenii could significantly inhibit the proliferation of cervical cancer cells in vitro (***p<0.001).

[0116] Embodiment 5:

[0117] See also Figure 6 , based on Examples 1-4 but with the difference that,

[0118] Effects of Lactobacillus crispatus and Lactobacillus jensenii on the migration of cervical cancer cells in vitro. The specific experimental steps are as follows:

[0119] 1) Seed about 5×105 SiHa cells in a 6-well plate and ensure that the wells are confluent overnight;

[0120] 2) On the second day, use the gun tip to make vertical scratches horizontally and vertically. The gun tip should be vertical and not tilted;

[0121] 3) Wash the cells three times with PBS, remove the scratched cells, add the supernatant of Lactobacillus crispatus and Lactobacillus jensenii in Example 4 to the culture medium at MOI=1:1000, and MRS is used as a control;

[0122] 4) Place the cells in a 37°C incubator, take samples at 0 and 48 hours, take photos, and analyze the images using ImageJ software.

[0123] Results Figure 6 The isolated Lactobacillus crispatus and Lactobacillus jensenii could significantly inhibit the migration of cervical cancer cells in vitro (****p<0.0001).

[0124] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and improved concepts of the present invention within the technical scope disclosed by the present invention, and they should be covered by the protection scope of the present invention.

Claims

1. A kind of vaginal Lactobacillus, which is deposited in the General Microbiology Center of China Microbial Culture Collection Committee, specifically including a kind of Lactobacillus jannoni; The deposit number of Lactobacillus jannaschii is CGMCC NO. 22802.

2. A vaginal Lactobacillus according to claim 1, It is characterized by, The Lactobacillus jannaschii was isolated from the vagina of healthy women.

3. A vaginal Lactobacillus according to claim 1, It is characterized by, The deposit number of Lactobacillus jannaschii is CGMCC NO. 22802, and its 16S rDNA sequence is: CCATTAGGCGGCTGGCTCCAAAGGTTACCTCACCGACTTTGGGTGTTACAAACTCTCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCAGCTTCGTGTAGGCGAGTTGCAGCCTACAGTCCGAACTGAGAACAGCTTTAAGAGATCCGCTTGCCTTCACAGGTTCGCTTCTCGTTGTACTGCCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCAATAGAGTGCCCAACTTAATGCTGGCAACTATTAACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTCTCTTTGTCCCCGAAGGGAAAACCTAATCTCTTAGGTGGTCAAAGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTGAGAGGCGGAAACCTCCCAACACTTAGCACTCATCGTTTACGGCATGGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCCTTCGCCACTGGTGTTCTTCCATATATCTACGCATTCCACCGCTACACATGGAGTTCCACTCTCCTCTTCTGCACTCAAGATTGACAGTTTCTGATGCAGTTCTTCGGTTGAGCCGAAGGCTTTCACATCAGACTTATCAATCCGCCTGCGCTCGCTTTACGCCCAATAAATCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGATTACCGTCAAATAAAGGCCAGTTACTACCTCTATCCTTCTTCACCAACAACAGAGCTTTACGATCCGAAAACCTTCTTCACTCACGCGGCGTTGCTCCATCAGACTTGCGTCCATTGTGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAGTCTCTCAACTCGGCTATGCATCATCGCCTTGGTAAGCCGTTACCTTACCAACTAGCTAATGCATCGCAGGTCCATCCTTTAGCGACAGCTTACGCCGCCTTTTAAACTTCTTTCATGCGAAAGTAGCTTTTATCCGGTATTAGCATCTGTTTCCAAATGGTATCCCAGACTTAAGGGCAGGTTACCCACGCGTTACTCACCCATCCGCCGCTAGCTTGTATCTATGTCCATTCCGAAGAATTTCAATAGGCAAGCTCGCTCGACTTGCATGTATTAGGCACGCCGCCAGCGTTCGTCCTGAGCCAGATTCCAAAC .

4. According to the specific application obtained by a kind of vaginal lactobacilli described in claim 1, It is characterized by, The Lactobacillus jannaschii can significantly inhibit the proliferation and migration of cervical cancer cells in vitro, and has important applications in the regulation of vaginal flora disorders and the prevention and treatment of cervical cancer.

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