Efficient plant leaf fertilizer containing fullerene C60 and preparation method thereof

Abstract

The invention provides an efficient plant leaf fertilizer containing fullerene C60 and a preparation method thereof, and relates to the technical field of leaf fertilizers. The efficient plant leaf fertilizer containing fullerene C60 is prepared from the following raw materials: a yeast extract, citric acid, xylitol, polyoxyethylene sorbitan fatty acid ester, glycerol, soybean small peptide chelate elements, plant organic selenium and modified fullerene C60. The preparation method comprises the following steps: stirring the yeast extract, citric acid, xylitol, polyoxyethylene sorbitan fatty acid ester, glycerol and deionized water at 30 DEG C, adding the modified fullerene C60, stirring, adding the soybean small peptide chelate element and the plant organic selenium, and stirring to obtain the efficient plant leaf fertilizer containing fullerene C60. The prepared foliar fertilizer increases the hundred-bud weight and yield of tea trees.

Description

Technical Field

[0001] The invention relates to the technical field of foliar fertilizers, and in particular to a high-efficiency plant foliar fertilizer containing fullerene C60 and a preparation method thereof. Background Art

[0002] Acidic land for growing tea trees is widely distributed in my country, mainly concentrated in the southern region, especially the provinces south of the Yangtze River. Acidic soils are widely distributed in these areas, especially red soil and yellow soil, which are very suitable for the growth of tea trees. However, a considerable portion of tea garden soil has a pH value below 4.5, which is severely acidified soil. For example, the average soil pH value of tea gardens in Jiangxi Province is the lowest, at 3.96, while tea trees are acid-loving crops with an optimum soil pH value of 4.5-5.5. However, due to natural factors (soil parent material, climate conditions, topography, etc.) and human factors (excessive use of chemical fertilizers, unreasonable farming methods, etc.), many tea gardens are facing soil acidification. Soil acidification not only affects the growth of tea trees and the quality of tea leaves, but also reduces the soil's ability to adsorb nutrients, making certain nutrients (such as calcium and magnesium) more easily lost, thereby affecting the nutrient absorption and growth and development of tea trees. It may also lead to ecological and environmental problems such as decreased soil fertility and increased heavy metal pollution. Foliar fertilizers mainly enter the leaves through the pores of the leaves and the gaps between the molecules of the cuticle compounds to provide the tea trees with the nutrients they need. This method is not affected by the soil. The influence of nutrient leaching, fixation and transformation, high nutrient utilization rate, good fertilization efficiency, and various types of foliar fertilizers, including macronutrient foliar fertilizers (such as nitrogen, phosphorus, and potassium), trace element foliar fertilizers (such as manganese, magnesium, boron, and zinc), and humic acid foliar fertilizers, etc. However, the formula of foliar fertilizers on the market does not fully take into account the complex acidic soil environment. The nutrients in many foliar fertilizers on the market are difficult to be efficiently absorbed and utilized by tea trees. In order to reduce R&D costs and expand sales, most foliar fertilizers on the market are broad-spectrum products, lacking special formulas for the growth characteristics of tea trees and acidic soil environments, resulting in inaccurate nutrient supply. Some foliar fertilizers are prone to chemical changes in an acidic environment, resulting in the ineffectiveness of the effective ingredients or the production of substances harmful to tea trees, which cannot fully improve the fertilizer efficiency and crop stress resistance, resulting in a decrease in 100 bud weight and yield. Therefore, a new high-efficiency plant foliar fertilizer is urgently needed in the market. Summary of the invention

[0003] The present invention provides a high-efficiency plant foliar fertilizer containing fullerene C60 and a preparation method thereof, which are used to solve the problems raised in the above-mentioned background technology.

[0004] In order to solve the above technical problems, the present invention discloses a high-efficiency plant foliar fertilizer containing fullerene C60 and a preparation method thereof. The high-efficiency plant foliar fertilizer containing fullerene C60 comprises yeast extract, citric acid, xylitol, polyoxyethylene sorbitan fatty acid ester, glycerol, deionized water, soybean peptide chelated elements, plant organic selenium and modified fullerene C60.

[0005] Furthermore, the raw materials for preparation include, by weight, 40-60 parts of yeast extract, 2-10 parts of citric acid, 2-8 parts of xylitol, 1-10 parts of polyoxyethylene sorbitan fatty acid ester, 3-10 parts of glycerol, 10-40 parts of deionized water, 10-20 parts of soybean peptide chelated elements, 2-8 parts of plant organic selenium and 3-8 parts of modified fullerene C60.

[0006] Furthermore, the preparation method of the modified fullerene C60 includes: adding 300 parts of cerium nitrate hexahydrate to 1200 parts of acetone by weight to prepare a cerium nitrate solution, placing 400 parts of fullerene and 400 parts of cerium nitrate solution into a beaker containing 1200 parts of deionized water, reacting at room temperature for 4 hours, filtering, washing, and drying to obtain fullerene A, weighing 300 parts of fullerene A and 600 parts of oxidant hydrogen peroxide, placing them in a reaction device, reacting at 40°C for 4 hours, filtering, washing, and drying to obtain hydroxylated fullerene B, weighing 200 parts of hydroxylated fullerene B, 16 parts of β-(3,5-di-tert-butyl-4-hydroxyphenyl) propionyl chloride, 3 parts of catalyst triethylamine, and 800 parts of acetone, placing them in a reaction device, reacting at 50°C for 6 hours, filtering, washing, and drying to obtain modified fullerene C60.

[0007] Furthermore, the preparation method of the yeast extract comprises: adding water to brewer's yeast powder to prepare an 8% w / v yeast suspension; adding 2% w / v sodium chloride and 400U / mL papain, adjusting the pH to 7.0, and autolyzing at 45 degrees Celsius for 24 hours; inactivating the enzyme and centrifuging to obtain a yeast extract; ultrafiltration, vacuum concentration, and spray drying to obtain a powdered yeast extract;

[0008] Preferably, the spray drying air inlet temperature is 180°C and the air outlet temperature is 90°C.

[0009] Furthermore, the preparation method of the soybean peptide chelated element comprises:

[0010] S1. Pretreatment: weigh 1000 kg of soybean meal, add 10000 kg of water, prepare a protein solution with a concentration of 10%, then add 1.3% of the mass of soybean meal enzymatic hydrolysis accelerator (polyvinyl alcohol 1799 and reduced glutathione in a weight ratio of 5:3), and homogenize twice under 15 MPa to obtain a homogenized mixed soybean meal protein solution;

[0011] Performing homogenization twice under the condition of 15Mpa includes: performing a homogenization once on the mixed soybean meal protein liquid before homogenization through a multi-stage variable diameter pipeline and a micro jet high pressure homogenizer under the condition of 15Mpa; and partially retaining the high pressure through the primary circulation temporary storage chamber, and then pressurizing to 15Mpa, and performing a second homogenization; reducing the pre-pressurization pressure; improving the micro jet high pressure homogenization efficiency; and offsetting the wear of the cavity caused by the impact of some large particles in the mixed soybean meal protein liquid before homogenization;

[0012] S2, enzymatic hydrolysis reaction: the pH of the mixed soybean meal protein solution obtained in step S1 is adjusted to 8 with 2 mol / L NaOH and HCl solution, and then a composite protease is added, and after enzymatic hydrolysis at 40°C for 3 hours, it is heated to 80°C, the enzyme is inactivated for 10 minutes, and then centrifuged at 4000 rpm / min for 20 minutes, the supernatant is collected, and the soybean peptide powder is obtained by freeze-drying;

[0013] S3, chelation reaction: weigh 900 kg of the soybean peptide powder obtained in step S2, add 9000 kg of water and stir evenly to prepare a soybean peptide solution with a concentration of 10%, add 1 part of antioxidant and 5 parts of ferrous sulfate, 3 parts of zinc acetate and 2 parts of manganese chloride to 100 parts of the 10% soybean peptide solution, stir evenly, then add 0.2% of the weight of the soybean peptide powder antioxidant, mix evenly, adjust the pH to 6 with 20% sodium hydroxide, start timing when heated to 45°C, react for 0.4h to obtain the soybean peptide chelated element.

[0014] Furthermore, the composite protease is composed of alkaline protease, neutral protease and trypsin according to the enzyme activity ratio of 3:2:1, and the total enzyme activity of the composite protease is 4000U / g soybean meal. Furthermore, the particle size of the rice husk is 60-80 mesh, and the fermentation time is 20-25 days to obtain an organic auxiliary material.

[0015] Furthermore, the antioxidant consists of sodium D-isoascorbate, astragalus polysaccharide and sodium sulfite in a weight ratio of 7:2:4.

[0016] Furthermore, the preparation method of plant organic selenium includes: mixing rice stems and leaves with rapeseed stems and leaves in a mass ratio of 1:3, crushing through a 100-mesh sieve, adding 30 times the mass of distilled water to the crushed selenium-rich plant stem and leaf powder, extracting at 80°C for 6 hours, then setting 800kHz ultrasonic-assisted extraction for 60 minutes, cooling to room temperature, filtering to remove the filter residue, taking the filtrate and adding 1 / 2 volume of 60v / v% ethanol thereto, precipitating with alcohol for 24 hours, taking the solution after alcohol precipitation, evaporating and concentrating to obtain plant organic selenium.

[0017] Furthermore, the preparation method of the high-efficiency plant foliar fertilizer containing fullerene C60 includes stirring yeast extract, citric acid, xylitol, polyoxyethylene sorbitan fatty acid ester, glycerol and deionized water, adding modified fullerene C60, stirring, adding soybean peptide chelated elements and plant organic selenium, and stirring to obtain a high-efficiency plant foliar fertilizer containing fullerene C60.

[0018] Furthermore, the method for applying the high-efficiency plant foliar fertilizer containing fullerene C60 is to spray it once on the new leaves and the back of the leaves of the tea tree before the tea heads sprout, and spray it once on the leaves of the tea tree with one bud and three leaves about 2 weeks before picking tea. The foliar fertilizer applied each time is diluted 400 to 500 times, the first spraying amount is 100L of aqueous solution per 500 square meters, and the second spraying amount is 50L of aqueous solution per 500 square meters. The spraying time is on a cloudy day, when the sun is about to set, or in the evening.

[0019] Compared with the prior art, the present invention provides a high-efficiency plant foliar fertilizer containing fullerene C60 and a preparation method thereof, which has the following beneficial effects:

[0020] (1) Hydroxylated fullerene C60 improves its dispersibility in water and wettability on plant leaves, which helps the fullerene C60 in foliar fertilizer to better contact the leaf surface, thereby accelerating its penetration process. Fullerene C60 has excellent antioxidant properties, which can remove free radicals in plants and reduce the damage of oxidative stress to plants. This antioxidant property may help protect leaf cells from damage, thereby maintaining the integrity of stomata and cuticles and promoting the absorption of foliar fertilizers;

[0021] (2) When hydroxylated fullerene C60 is used in combination with the iron, zinc and manganese in soybean peptide chelated elements in foliar fertilizers, and selenium in plant organic selenium, they produce a synergistic effect and enhance the effectiveness of the foliar fertilizers. This synergistic effect makes the nutrients in the foliar fertilizers easier for plants to absorb and utilize, thereby improving the fertilization effect.

[0022] (3) Polyoxyethylene sorbitan fatty acid ester is a more environmentally friendly and biodegradable surfactant. Glycerol as a penetrant can reduce the surface tension of leaves, making it easier for foliar fertilizer to penetrate into the leaves. The two work together to make foliar fertilizer easier to disperse evenly and enhance penetration.

[0023] (4) Xylitol has low hygroscopicity and excellent moisture resistance. It is used as a desiccant for foliar fertilizer. Soybean peptide chelated elements contain trace elements, which meet the comprehensive nutritional needs of tea. These trace elements are added in chelated form to improve their stability and absorption rate.

[0024] (5) The cerium nitrate hexahydrate used to prepare the modified fullerene C60 contains cerium, which enhances the growth ability of plants by affecting the physiological metabolic processes of plants, such as photosynthesis and respiration. In tea trees, this may be manifested as a higher growth rate, more luxuriant branches and leaves, and stronger vitality. By improving the nutrient absorption and metabolic processes of tea trees, the quality of tea leaves is indirectly improved, including the color, aroma, and taste of tea leaves. The chlorophyll content in plant leaves can be increased, which may affect the color and taste of tea leaves and enhance the stress resistance of plants, including drought resistance, cold resistance, and disease resistance. In the process of tea tree cultivation, the improvement of these stress resistances helps to reduce the occurrence of pests and diseases and reduce the use of pesticides, thereby improving the safety and quality of tea leaves and helping tea trees better absorb nutrients in the soil, such as nitrogen, phosphorus, and potassium. These nutrients are an important material basis for the growth and quality formation of tea leaves. By promoting the absorption and utilization of nutrients, cerium contributes to the growth and development of tea leaves. Plant organic selenium contains selenium, which promotes the growth of tea trees, improves the disease resistance, drought resistance and cold resistance of tea trees, reduces the occurrence of diseases and insect pests, and thus increases tea yields. Selenium can also promote the photosynthesis of tea leaves, improve the photosynthetic efficiency of tea leaves, and further promote the growth and development of tea leaves. Selenium has an antagonistic effect on heavy metals, and can reduce the content of heavy metals such as cadmium and arsenic in tea leaves, reduce the pollution risk of tea leaves, and also reduce pesticide residues in tea leaves, thereby improving the safety of tea leaves. The various components work synergistically and work together on the growth of tea trees, increasing the 100 bud weight and yield of tea trees. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0026] Figure 1 This is a diagram of an embodiment of a reaction device for a high-efficiency plant foliar fertilizer containing fullerene C60 and a preparation method thereof as described in the present invention. DETAILED DESCRIPTION

[0027] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0028] In addition, in the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes, and do not specifically refer to the order or sequence, nor are they used to limit the present invention. They are only used to distinguish components or operations described with the same technical terms, and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions and technical features between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0029] Unless otherwise specified, the examples and comparative examples are parallel tests with the same components, component contents, preparation steps and preparation parameters. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples are analytical reagents (AR) unless otherwise specified. Parts are parts by weight unless otherwise specified, and the materials are purchased from commercial channels.

[0030] Fullerene C60 was purchased from Sigma-Aldrich, CAS No.: 99685-96-8, with a molecular weight of 720.64 and a purity of 99.5%; brewer's yeast powder was purchased from Zhuhai Hewei Biotechnology Co., Ltd., with an effective substance content of ≥90%; L-reduced glutathione was purchased from Sigma-Aldrich, CAS No.: 70-18-8, with a purity of ≥98%; alkaline protease was purchased from Sigma-Aldrich, CAS No.: 9014-01-1, and neutral protease was purchased from Sigma-Aldrich, UNSPSC code: 12352200; trypsin was purchased from Sigma-Aldrich, CAS No.: 9002-07-7; papain was purchased from Sigma-Aldrich, CAS No.: 9001-73-4; soybean meal was purchased from Yuncheng Jinyu Feed Co., Ltd., with a crude protein content of ≥45% and a moisture content of 12%.

[0031] Example 1

[0032] The preparation method of high-efficiency plant foliar fertilizer containing fullerene C60 comprises the following specific steps:

[0033] Preparation of modified fullerene C60:

[0034] 300 g of cerium nitrate hexahydrate was added to 1.2 kg of acetone to prepare a cerium nitrate solution. 400 g of fullerene and 400 g of cerium nitrate solution were placed in a beaker containing 1.2 kg of deionized water, and reacted at room temperature for 4 hours. The mixture was filtered, washed, and dried to obtain fullerene A. 300 g of fullerene A and 600 g of oxidant hydrogen peroxide were weighed and placed in a reaction device. The mixture was reacted at 40° C. for 4 hours. The mixture was filtered, washed, and dried to obtain hydroxylated fullerene B. 200 g of hydroxylated fullerene B, 16 g of β-(3,5-di-tert-butyl-4-hydroxyphenyl)propionyl chloride, 3 g of catalyst triethylamine, and 800 g of acetone were weighed and placed in a reaction device. The mixture was reacted at 50° C. for 6 hours. The mixture was filtered, washed, and dried to obtain modified fullerene C60.

[0035] Prepare yeast extract:

[0036] For waste beer yeast: add water, stir evenly, sieve, centrifuge, repeat 3-4 times, and obtain yeast mud without obvious foam and wine smell; add sodium bicarbonate of 0.5% of the weight of yeast mud, mix evenly, debitter for 2 hours, and centrifuge to remove supernatant; add water to the yeast mud precipitate obtained by centrifugation to prepare a 12% w / v yeast suspension; add 2% w / v sodium chloride and 400U / mL papain, adjust the pH to 6.5, and autolyze at 45 degrees Celsius for 24 hours; inactivate the enzyme, centrifuge to obtain yeast extract; ultrafiltration, vacuum concentration, spray (spray drying inlet temperature 180°C, outlet temperature 90°C) drying to obtain powdered yeast extract.

[0037] For brewer's yeast powder: add water to brewer's yeast powder to prepare 8% w / v yeast suspension; add 2% w / v sodium chloride and 400U / mL papain, adjust the pH to 7.0, and autolyze at 45 degrees Celsius for 24 hours; inactivate the enzyme, centrifuge to obtain yeast extract; ultrafilter, vacuum concentrate, and spray dry to obtain powdered yeast extract.

[0038] The reaction equipment includes: a reaction vessel 31, a reaction vessel cover 32, a reaction vessel shaking device 33, a heating and heat preservation device 34, a timer 35 and a reaction controller 36; the reaction vessel is a corrosion-resistant heat-conducting container for containing reaction substances; the reaction vessel cover is used to seal the reaction vessel; the reaction vessel shaking device is used to shake the reaction vessel containing the reaction substances to accelerate the reaction speed and improve the reaction uniformity; the reaction vessel shaking device includes: a swing arm 331, a deflection plate 332 and a vessel clamp 333; the reaction vessel is fixed by the vessel clamp; the swing arm is driven by the rotation of the deflection plate to swing and shake; the heating and heat preservation device is used to heat the reaction vessel and maintain the set reaction temperature; the heating and heat preservation device includes: a heating belt 341, a heat preservation cover 342 and an electronic thermometer 343; a heating wire is arranged in the heating belt, attached to the inner wall of the heat preservation cover, and electrically heated by the heating belt; the heat preservation cover is a transparent cover body, which covers the outside of the reaction vessel, and is used to maintain the temperature during the reaction process, saving heating energy consumption during a long reaction process; the electronic thermometer is used to detect the reaction heating temperature; the timer is used to time according to the set reaction time; the reaction controller is electrically connected to the reaction vessel shaking device, the heating and heat preservation device and the timer; the reaction controller controls the start and stop of the swinging and shaking device and the swinging and shaking amplitude of the reaction vessel shaking device and the heating and heat preservation device, and according to the timing of the timer, after the set reaction time is reached, a reaction time limit reminder is issued.

[0039] Preparation of soybean peptide chelated elements:

[0040] S1. Pretreatment: weigh 1000 kg of soybean meal, add 10000 kg of water, prepare a protein solution with a concentration of 10%, then add 1.3% of the mass of soybean meal enzymatic hydrolysis accelerator (polyvinyl alcohol 1799 and reduced glutathione in a weight ratio of 5:3), and homogenize twice under 15 MPa to obtain a homogenized mixed soybean meal protein solution;

[0041] The homogenization treatment is performed twice under the condition of 15Mpa, including: under the condition of 15Mpa, the mixed soybean meal protein liquid before homogenization is pressed into the high-pressure input pipe through the multi-stage variable diameter pipe; the first stage pipe diameter of the multi-stage variable diameter pipe is the largest, and the diameters of the second stage pipe to the last stage pipe are successively reduced, and the pressure in the last stage pipe of the multi-stage variable diameter pipe reaches 15Mpa; the diameter of the last stage pipe is the same as the diameter of the high-pressure input pipe of the micro-jet high-pressure homogenizer; the micro-jet high-pressure homogenizer is provided with a plurality of high-pressure input pipes, and the plurality of high-pressure input pipes are convected in pairs in the micro-jet high-pressure homogenizer, and the high-pressure convection impact speed is doubled to form a higher speed. Convection high-pressure impact; and offset the wear of some large particles in the mixed soybean meal protein liquid before homogenization; after two convections and homogenization, they are collected in the primary circulation temporary storage chamber; there is still some retained high pressure in the primary circulation temporary storage chamber, and it is introduced into the secondary circulation pressure pump, and then pressurized to 15Mpa on the basis of retaining high pressure, and introduced into the high-pressure input pipe through a one-way valve for secondary homogenization treatment; greatly reduce the pre-pressurization power consumption of the second homogenization treatment; reduce the pre-pressurization pressure; significantly improve the efficiency of micro-jet high-pressure homogenization; and offset the wear of some large particles in the mixed soybean meal protein liquid before homogenization. The impact of the cavity, improve the service life of the micro-jet high-pressure homogenizer equipment;

[0042] S2, enzymatic hydrolysis reaction: the pH of the mixed soybean meal protein solution obtained in step S1 is adjusted to 8 with 2 mol / L NaOH and HCl solution, and then a composite protease (alkaline protease, neutral protease and trypsin are composed according to the enzyme activity ratio of 3:2:1, and the total enzyme activity of the composite protease is 4000U / g soybean meal) is added, and after enzymatic hydrolysis at 40°C for 3h, it is heated to 80°C, the enzyme is inactivated for 10min, and then centrifuged at 4000rpm / min for 20min, the supernatant is collected, and freeze-dried to obtain soybean peptide powder;

[0043] S3, chelation reaction: weigh 900 kg of the soybean peptide powder obtained in step S2, add 9000 kg of water and stir evenly to prepare a soybean peptide solution with a concentration of 10%, add 1 part of antioxidant (D-sodium isoascorbate, astragalus polysaccharide and sodium sulfite in a weight ratio of 7:2:4) and 5 parts of ferrous sulfate, 3 parts of zinc acetate and 2 parts of manganese chloride to 100 parts of the 10% soybean peptide solution, stir evenly, and then add 0.2% of the weight of the soybean peptide powder. After mixing evenly, adjust the pH to 6 with 20% sodium hydroxide, start timing when heated to 45°C, and react for 0.4h to obtain the soybean peptide chelated element.

[0044] Preparation of plant organic selenium:

[0045] The dried stems and leaves of selenium-rich plants (a mixture of rice stems and leaves and rapeseed stems and leaves, with a mass ratio of 1:3) are crushed through a 100-mesh sieve, and 30 times the mass of distilled water is added to the crushed selenium-rich plant stem and leaf powder, and extracted at 80°C for 6 hours, followed by 800kHz ultrasonic-assisted extraction for 60 minutes. The mixture is cooled to room temperature, filtered to remove the residue, and 1 / 2 volume of 60v / v% ethanol is added to the filtrate. The mixture is precipitated for 24 hours, and the solution after the precipitation is taken and evaporated and concentrated to obtain plant organic selenium.

[0046] Preparation of high-efficiency plant foliar fertilizer:

[0047] At 30°C, 50 parts of yeast extract, 5 parts of citric acid, 6 parts of xylitol, 8 parts of polyoxyethylene sorbitan fatty acid ester, 8 parts of glycerol and 20 parts of deionized water were mixed in a weight ratio and stirred for 30 minutes. 6 parts of modified fullerene C60 were added and stirred for 30 minutes. 12 parts of soybean peptide chelated elements and 5 parts of plant organic selenium were added and stirred for 60 minutes to obtain a high-efficiency plant foliar fertilizer containing fullerene C60.

[0048] The method of applying high-efficiency plant foliar fertilizer containing fullerene C60 is to spray it once on the new leaves and the back of the leaves of the tea tree before the tea heads sprout. About 2 weeks before tea picking, it is mainly sprayed once on the leaves of the tea tree with one bud and three leaves. The foliar fertilizer applied each time is diluted 400 to 500 times. The first spraying amount is 100L of aqueous solution per 500 square meters, and the second spraying amount is 50L of aqueous solution per 500 square meters. The spraying time is on cloudy days, when the sun is about to set, or in the evening.

[0049] Example 2

[0050] The difference from Example 1 is that a high-efficiency plant foliar fertilizer is prepared, and the rest is the same as Example 1.

[0051] Preparation of high-efficiency plant foliar fertilizer:

[0052] At 30°C, 40 parts of yeast extract, 2 parts of citric acid, 2 parts of xylitol, 1 part of polyoxyethylene sorbitan fatty acid ester, 3 parts of glycerol and 10 parts of deionized water were mixed in a weight ratio and stirred for 30 minutes. 3 parts of modified fullerene C60 were added and stirred for 30 minutes. 10 parts of soybean small peptide chelated elements and 2 parts of plant organic selenium were added and stirred for 60 minutes to obtain a high-efficiency plant foliar fertilizer containing fullerene C60.

[0053] Example 3

[0054] The difference from Example 1 is that a high-efficiency plant foliar fertilizer is prepared, and the rest is the same as Example 1.

[0055] Preparation of high-efficiency plant foliar fertilizer:

[0056] At 30°C, 60 parts of yeast extract, 10 parts of citric acid, 8 parts of xylitol, 10 parts of polyoxyethylene sorbitan fatty acid ester, 10 parts of glycerol and 40 parts of deionized water were mixed in a weight ratio and stirred for 30 minutes. 8 parts of modified fullerene C60 were added and stirred for 30 minutes. 20 parts of soybean small peptide chelated elements and 8 parts of plant organic selenium were added and stirred for 60 minutes to obtain a high-efficiency plant foliar fertilizer containing fullerene C60.

[0057] Comparative Example 1

[0058] The difference from Example 1 is that the modified fullerene C60 is missing in equal parts by weight, and the other parts are the same.

[0059] Comparative Example 2

[0060] The difference from Example 1 is that an equal weight portion of polyoxyethylene sorbitan fatty acid ester is missing, and the other contents are the same.

[0061] Comparative Example 3

[0062] The difference from Example 1 is that an equal weight portion of glycerol is missing, and the other parts are the same.

[0063] Comparative Example 4

[0064] The difference from Example 1 is that the soybean peptide chelated element is missing in equal parts by weight, and the other parts are the same.

[0065] Comparative Example 5

[0066] The difference from Example 1 is that an equal weight portion of plant organic selenium is missing, and the other contents are the same.

[0067] Comparative Example 6

[0068] The difference from Example 1 is that equal weight portions of polyoxyethylene sorbitan fatty acid ester and glycerol are missing, and the other contents are the same.

[0069] Comparative Example 7

[0070] The difference from Example 1 is that equal weight portions of soybean peptide chelated elements and plant organic selenium are missing, and the rest are the same.

[0071] Comparative Example 8

[0072] The difference from Example 1 is that when preparing the soybean peptide chelated element, equal parts of ferrous sulfate, zinc acetate and manganese chloride are not added, and the rest are the same.

[0073] Performance Testing

[0074] Field fertilizer efficiency experiment: The fertilizer prepared in Example 1 and the comparative example was sprayed once on the new leaves and the back of the leaves of the tea tree before the tea heads sprouted. About 2 weeks before tea picking, it was mainly sprayed once on the leaves of one bud and three leaves of the tea tree. The foliar fertilizer applied each time was diluted 400 to 500 times. The first spraying amount was 100L of aqueous solution per 500 square meters, and the second spraying amount was 50L of aqueous solution per 500 square meters. The spraying time was cloudy. The tea planted was Yinghong No. 9, hilly land, slightly acidic soil pH 5.0, and the soil contained 0.2% lime. Double rows were planted: the large row spacing was 150 cm, the small row spacing was 30 cm, the plant spacing was 45 cm, and 2000 tea seedlings were planted per mu. The results are shown in Table 1. The blank control group was a blank control sprayed with clean water.

[0075] Table 1

[0076] 100 bud weight(g) Yield (kg / mu) Example 1 214 223.4 Comparative Example 1 204 212.1 Comparative Example 2 208 218.7 Comparative Example 3 210 220.3 Comparative Example 4 199 204.5 Comparative Example 5 201 208.4 Comparative Example 6 202 210.8 Comparative Example 7 197 202.1 Comparative Example 8 200 206.7 Blank control 196 200.3

[0077] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A high-efficiency plant foliar fertilizer containing fullerene C60, wherein the raw materials for preparation include yeast extract, citric acid, xylitol, polyoxyethylene sorbitan fatty acid ester, glycerol, deionized water, soybean peptide chelated elements, plant organic selenium and modified fullerene C60.

2. The high-efficiency plant foliar fertilizer containing fullerene C60 according to claim 1, characterized in that: The raw materials for preparation include 40-60 parts of yeast extract, 2-10 parts of citric acid, 2-8 parts of xylitol, 1-10 parts of polyoxyethylene sorbitan fatty acid ester, 3-10 parts of glycerol, 10-40 parts of deionized water, 10-20 parts of soybean peptide chelated elements, 2-8 parts of plant organic selenium and 3-8 parts of modified fullerene C60 in parts by weight.

3. The high-efficiency plant foliar fertilizer containing fullerene C60 according to claim 1 or 2, characterized in that: The preparation method of the modified fullerene C60 comprises: adding 300 parts of cerium nitrate hexahydrate to 1200 parts of acetone by weight to prepare a cerium nitrate solution, placing 400 parts of fullerene and 400 parts of the cerium nitrate solution into a beaker containing 1200 parts of deionized water, reacting at room temperature for 4 hours, filtering, washing, and drying to obtain fullerene A, weighing 300 parts of fullerene A and 600 parts of oxidant hydrogen peroxide, placing them in a reaction device, reacting at 40°C for 4 hours, filtering, washing, and drying to obtain hydroxylated fullerene B, weighing 200 parts of hydroxylated fullerene B, 16 parts of β-(3,5-di-tert-butyl-4-hydroxyphenyl) propionyl chloride, 3 parts of catalyst triethylamine, and 800 parts of acetone, placing them in a reaction device, reacting at 50°C for 6 hours, filtering, washing, and drying to obtain modified fullerene C60.

4. The high-efficiency plant foliar fertilizer containing fullerene C60 according to claim 1 or 2, characterized in that: The preparation method of the yeast extract comprises: adding water to brewer's yeast powder to prepare an 8% w / v yeast suspension; adding 2% w / v sodium chloride and 400U / mL papain, adjusting the pH to 7.0, and autolyzing at 45 degrees Celsius for 24 hours; inactivating the enzyme, centrifuging to obtain a yeast extract; ultrafiltration, vacuum concentration, and spray drying to obtain a powdered yeast extract; Preferably, the spray drying air inlet temperature is 180°C and the air outlet temperature is 90°C.

5. The high-efficiency plant foliar fertilizer containing fullerene C60 according to claim 1 or 2, characterized in that: The preparation method of the soybean peptide chelated element comprises: S1. Pretreatment: weigh 1000 kg of soybean meal, add 10000 kg of water, prepare a protein solution with a concentration of 10%, then add 1.3% of the mass of soybean meal enzymatic hydrolysis accelerator (polyvinyl alcohol 1799 and reduced glutathione in a weight ratio of 5:3), and homogenize twice under 15 MPa to obtain a homogenized mixed soybean meal protein solution; Performing homogenization twice under the condition of 15Mpa includes: performing a homogenization once on the mixed soybean meal protein liquid before homogenization through a multi-stage variable diameter pipeline and a micro jet high pressure homogenizer under the condition of 15Mpa; and partially retaining the high pressure through the primary circulation temporary storage chamber, and then pressurizing to 15Mpa, and performing a second homogenization; reducing the pre-pressurization pressure; improving the micro jet high pressure homogenization efficiency; and offsetting the wear of the cavity caused by the impact of some large particles in the mixed soybean meal protein liquid before homogenization; S2, enzymatic hydrolysis reaction: the pH of the mixed soybean meal protein solution obtained in step S1 is adjusted to 8 with 2 mol / L NaOH and HCl solution, and then a composite protease is added, and after enzymatic hydrolysis at 40°C for 3 hours, it is heated to 80°C, the enzyme is inactivated for 10 minutes, and then centrifuged at 4000 rpm / min for 20 minutes, the supernatant is collected, and the soybean peptide powder is obtained by freeze-drying; S3, chelation reaction: weigh 900 kg of the soybean peptide powder obtained in step S2, add 9000 kg of water and stir evenly to prepare a soybean peptide solution with a concentration of 10%, add 1 part of antioxidant and 5 parts of ferrous sulfate, 3 parts of zinc acetate and 2 parts of manganese chloride to 100 parts of the 10% soybean peptide solution, stir evenly, then add 0.2% of the weight of the soybean peptide powder antioxidant, mix evenly, adjust the pH to 6 with 20% sodium hydroxide, start timing when heated to 45°C, react for 0.4h to obtain the soybean peptide chelated element.

6. The high-efficiency plant foliar fertilizer containing fullerene C60 according to claim 5, characterized in that: The composite protease is composed of alkaline protease, neutral protease and trypsin according to an enzyme activity ratio of 3:2:1, and the total enzyme activity of the composite protease is 4000U / g soybean meal.

7. The high-efficiency plant foliar fertilizer containing fullerene C60 according to claim 5, characterized in that: The antioxidant consists of sodium D-isoascorbate, astragalus polysaccharide and sodium sulfite in a weight ratio of 7:2:

4.

8. The high-efficiency plant foliar fertilizer containing fullerene C60 according to claim 1 or 2, characterized in that: The preparation method of plant organic selenium comprises: mixing rice stems and leaves with rape stems and leaves in a mass ratio of 1:3, crushing and passing through a 100-mesh sieve, adding 30 times the mass of distilled water to the crushed selenium-rich plant stem and leaf powder, extracting at 80°C for 6 hours, then setting 800kHz ultrasonic-assisted extraction for 60 minutes, cooling to room temperature, filtering to remove filter residue, adding 1 / 2 volume of 60v / v% ethanol to the filtrate, precipitating with alcohol for 24 hours, taking the solution after alcohol precipitation, evaporating and concentrating, and obtaining plant organic selenium.

9. A method for preparing a high-efficiency plant foliar fertilizer containing fullerene C60 according to any one of claims 1 to 8, characterized in that: The process comprises stirring yeast extract, citric acid, xylitol, polyoxyethylene sorbitan fatty acid ester, glycerin and deionized water, adding modified fullerene C60, stirring, adding soybean peptide chelated elements and plant organic selenium, and stirring to obtain a high-efficiency plant foliar fertilizer containing fullerene C60.

10. The high-efficiency plant foliar fertilizer containing fullerene C60 according to any one of claims 1 to 8, characterized in that: The method for applying the high-efficiency plant foliar fertilizer containing fullerene C60 is as follows: spraying it once on the new leaves and the back of the leaves of the tea tree before the tea heads sprout, and spraying it once about 2 weeks before picking the tea, mainly on the leaves of the tea tree with one bud and three leaves. The foliar fertilizer applied each time is diluted 400 to 500 times, the first spraying amount is 100L of aqueous solution per 500 square meters, and the second spraying amount is 50L of aqueous solution per 500 square meters. The spraying time is on a cloudy day, when the sun is about to set, or in the evening.

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