Device for preparing platelet-rich fibrin and extraction method

Abstract

The invention discloses a device for preparing platelet-rich fibrin and an extraction method. The device comprises a vacuum blood collection tube. The device has the beneficial effects that when the gel is extracted after the injectable PRF gel is prepared, the rubber plug is firstly opened, and then the piston is moved downwards through the needle-free injector, so that the piston is in contact with the injectable PRF gel, and the injectable PRF gel can be conveniently extracted under the condition that the injectable PRF gel is not exposed; the injectable PRF gel is used for extracting the injectable PRF gel, pollution to the external environment during extraction of the injectable PRF gel is avoided, meanwhile, when the liquid injectable PRF gel is extracted, a piston is also directly moved downwards through a corresponding needle-free injector, so that the separated liquid injectable PRF gel is extracted, and the extraction efficiency is improved. The injectable PRF gel and the liquid substance are extracted by adopting a needle-free injector, so that the risk that a worker is accidentally punctured when the injectable PRF gel and the liquid substance are extracted can be avoided, and the extraction process of the injectable PRF is safer and more reliable.

Description

Technical Field

[0001] The invention relates to the technical field of platelet-rich fibrin extraction, and in particular to a device for preparing platelet-rich fibrin and an extraction method. Background Art

[0002] Platelet-rich fibrin (PRF) is a second-generation platelet concentrate derived from autologous blood. It is prepared by centrifuging whole blood and does not require the addition of any exogenous agents, thus avoiding the risk of immune response and disease transmission. Currently, PRF has been widely used in oral and maxillofacial surgery, orthopedics, plastic surgery, sports medicine, skin beauty and other fields.

[0003] There are two forms of PRF: APRF and IPRF. APRF (gel form) is rich in platelets, leukocytes, and especially various growth factors and cytokines, which can promote the migration, proliferation, and differentiation of stem cells, as well as the formation of new blood vessels and the synthesis of bone collagen. IPRF (liquid form) extracts injectable PRF by changing the extraction process, which contains a variety of growth factors, such as platelets, transforming growth factor, vascular endothelial growth factor, and insulin-like growth factor.

[0004] APRF preparation process: draw venous blood and place it in a sterile centrifuge tube without anticoagulant components; then immediately place the centrifuge tube in a centrifuge for centrifugation; after centrifugation, let it stand until the blood sample is divided into three layers. Between the red blood cell fragments at the bottom and the light yellow clear liquid platelet plasma at the top, take out the light yellow gel in the middle layer, which is platelet-rich fibrin. Place the PRF gel in a dry and sterilized container for 10 minutes to allow it to shrink naturally and release the serum inside.

[0005] Preparation method of IPRF: draw venous blood and place it in a sterile centrifuge tube without anticoagulant components; then immediately place the centrifuge tube in a centrifuge and set a lower centrifugal speed and time for centrifugation; after centrifugation, the blood is separated into layers, the lower layer is red blood cells, and the upper layer is injectable PRF.

[0006] At present, there are still some problems in the preparation and extraction process of PRF: PRF gel needs to be clamped with tweezers or other tools when extracting it. If it is not taken out thoroughly, it is easy to break or crush it. If it is thrown out directly, it is easy to splash serum and cause contamination. The operation process is relatively complicated. In addition, the PRF gel is directly exposed to the laboratory environment when it is taken out, and there is also a risk of contamination. Injectable PRF cannot further concentrate the concentration of platelets and growth factors. In addition, an injection needle is required for extraction, which can easily cause puncture injuries to people. Summary of the invention

[0007] The purpose of the present invention is to provide a device for preparing platelet-rich fibrin and an extraction method in order to solve the above-mentioned problems. PRF can be extracted without using instruments such as injection needles or tweezers. The extraction method is simpler and can extract injectable PRF with higher concentrations of platelets and growth factors. It also avoids punctures to personnel and possible contamination of the laboratory environment caused by exposure of PRF gel.

[0008] The present invention achieves the above-mentioned purpose through the following technical solutions:

[0009] A device for preparing platelet-rich fibrin comprises a vacuum blood collection tube, a rubber plug is installed at the top of the vacuum blood collection tube, a piston is installed under the rubber plug in the vacuum blood collection tube, a needleless syringe is arranged above the vacuum blood collection tube, and a gelator is arranged below the vacuum blood collection tube.

[0010] Furthermore, the rubber plug is plugged into the vacuum blood collection tube. The rubber plug is a medical rubber plug, which is mainly used to seal the port of the vacuum blood collection tube to ensure the vacuum degree of the vacuum blood collection tube when collecting blood.

[0011] Furthermore, the piston is composed of a plug body and a sealing ring on the outside of the plug body after installation, and a liquid extraction hole is opened in the middle of the piston, and fitting grooves are opened at both upper and lower ends of the piston. The piston can slide along the inner wall of the vacuum blood collection tube to discharge the air in the vacuum blood collection tube when extracting injectable PRF.

[0012] Furthermore, the liquid extraction hole passes through the piston, and the fitting groove is a conical groove structure. The size of the fitting groove matches the size of the cone head at the bottom end of the needle-free syringe, which can ensure a reliable seal between the end of the needle-free syringe and the piston.

[0013] Furthermore, the gelator is provided with a slot for placing the vacuum blood collection tube, and the gelator is an intelligent temperature-controlled gel device, which can process the injectable PRF gel under a constant temperature.

[0014] Further, the preparation process of injectable PRF gel is as follows:

[0015] Step 1: Use one end of the blood collection needle to perform venipuncture on the patient, and then insert the needle tube at the other end of the blood collection needle into the vacuum blood collection tube. Due to the vacuum in the tube, the required volume of blood is automatically collected;

[0016] Step 2: Place the vacuum blood collection tube after collecting blood into the centrifuge. If necessary, take a balance tube and place it face to face with the vacuum blood collection tube into the centrifuge to ensure that the centrifuge remains balanced. Centrifuge after balancing.

[0017] Step 3, placing the centrifuged vacuum blood collection tube into a gelator and heating it to make it gel;

[0018] Step 4. After the vacuum blood collection tube is heated and cooled, remove the rubber stopper, take a needle-free syringe, insert the cone of the needle-free syringe into the hole in the middle of the piston in the tube, and make the cone on the needle-free syringe fit tightly with the fitting groove, then hold the curled edge of the needle-free syringe jacket and push the piston downward to first expel the air in the vacuum blood collection tube and then extract the PRF gel into the needle-free syringe.

[0019] Further, the preparation process of liquid injectable PRF gel is as follows:

[0020] Step 1: Use a blood collection needle to perform venipuncture, and then insert the needle tube at the other end of the blood collection needle into the vacuum blood collection tube. Due to the vacuum in the tube, the required volume of blood is automatically collected;

[0021] Step 2: Place the vacuum blood collection tube with collected blood into the centrifuge. If necessary, take a balance tube and place it face to face with the vacuum blood collection tube into the centrifuge to ensure that the centrifuge remains balanced. Centrifuge after balancing.

[0022] Step 3: After the vacuum blood collection tube is centrifuged and taken out, remove the rubber stopper, take a needle-free syringe, insert the cone of the needle-free syringe into the liquid extraction hole in the middle of the piston in the tube, hold the curled edge of the needle-free syringe jacket and push the piston downward, first extract the air and the top layer of liquid in the vacuum blood collection tube, and then extract the injectable PRF with a higher concentration of platelets and growth factors into the needle-free syringe.

[0023] Furthermore, in step 3, the temperature of the gel is 75-90° C. and the gel time is 10-15 min, which can effectively ensure the gel quality of the injectable PRF.

[0024] The beneficial effects of the present invention are:

[0025] When extracting the injectable PRF gel after the preparation of the present invention, the rubber plug is first opened, and then the piston is moved downward by a needle-free syringe to ensure that the piston is in contact with the injectable PRF gel, so as to realize convenient extraction of the injectable PRF gel without being exposed, and avoid the contamination of the injectable PRF gel to the external environment during extraction. At the same time, when extracting liquid injectable PRF, the piston is directly moved downward by a corresponding needle-free syringe to extract the separated liquid injectable PRF. Since the injectable PRF gel and the liquid substance are extracted by a needle-free syringe, the risk of accidental stabbing of the staff can be avoided when extracting the injectable PRF, making the injectable PRF extraction process safer and more reliable. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a disassembled diagram of a device for preparing platelet-rich fibrin according to the present invention;

[0027] Figure 2 It is a main cross-sectional view of a vacuum blood collection tube in a device for preparing platelet-rich fibrin according to the present invention;

[0028] Figure 3 A flow chart of a device for preparing platelet-rich fibrin according to the present invention when preparing injectable PRF gel;

[0029] Figure 4 The present invention is a flow chart of a device for preparing platelet-rich fibrin in preparing liquid injectable PRF.

[0030] The following are the descriptions of the reference numerals:

[0031] 1. Needleless syringe; 2. Rubber stopper; 3. Vacuum blood collection tube; 4. Gel analyzer; 5. Piston; 6. Liquid extraction hole; 7. Fitting groove. DETAILED DESCRIPTION

[0032] A device for preparing platelet-rich fibrin and an extraction method, comprising a vacuum blood collection tube 3, a rubber plug 2 is installed at the top of the vacuum blood collection tube 3, a piston 5 is installed at the lower side of the rubber plug 2 in the vacuum blood collection tube 3, a needleless syringe 1 is arranged above the vacuum blood collection tube 3, and a gelator 4 is arranged below the vacuum blood collection tube 3. When extracting the gel after the injectable PRF gel is prepared, the rubber plug 2 is first opened, and then the piston 5 is moved down by the needleless syringe 1 to ensure that the piston 5 is in contact with the injectable PRF gel, so as to achieve the effect of not exposing the gel. The convenient extraction of the injectable PRF gel avoids the contamination of the external environment caused by the injectable PRF gel during extraction. At the same time, when extracting the liquid injectable PRF, the piston 5 is directly moved downward through the corresponding needle-free syringe 1 to extract the separated liquid injectable PRF. Since the needle-free syringe 1 is used to extract the injectable PRF gel and the liquid substance, the risk of accidental stabbing of the staff can be avoided when extracting the injectable PRF, making the injectable PRF extraction process safer and more reliable.

[0033] In this embodiment, the rubber plug 2 is plugged into the vacuum blood collection tube 3. The rubber plug 2 is a medical rubber plug. The rubber plug 2 is mainly used to seal the port of the vacuum blood collection tube 3 to ensure the vacuum degree of the vacuum blood collection tube 3 when collecting blood.

[0034] In this embodiment, the piston 5 is composed of a plug body and a sealing ring on the outside of the plug body after installation, and a liquid extraction hole 6 is opened in the middle of the piston 5, and fitting grooves 7 are opened at the upper and lower ends of the piston 5. The piston 5 can slide along the inner wall of the vacuum blood collection tube 3 to discharge the air in the vacuum blood collection tube 3 when extracting injectable PRF.

[0035] In this embodiment, the liquid extraction hole 6 passes through the piston 5, and the fitting groove 7 is a conical groove structure. The size of the fitting groove 7 matches the size of the cone head at the bottom end of the needle-free syringe 1, which can ensure a reliable seal between the end of the needle-free syringe 1 and the piston 5.

[0036] In this embodiment, the gelator 4 is provided with a slot for placing the vacuum blood collection tube 3 . The gelator 4 is an intelligent temperature-controlled gel device. The gelator 4 can process the injectable PRF gel under a constant temperature.

[0037] In this embodiment, the preparation process of injectable PRF gel is as follows:

[0038] Step 1: Use one end of a blood collection needle to perform venipuncture on the patient, and then insert the needle tube at the other end of the blood collection needle into the vacuum blood collection tube 3, and the required volume of blood is automatically collected due to the vacuum in the tube;

[0039] Step 2, put the vacuum blood collection tube 3 after collecting blood into the centrifuge, if necessary, take a balance tube and put it into the centrifuge face to face with the vacuum blood collection tube 3, ensure that the centrifuge remains balanced, and centrifuge after balancing;

[0040] Step 3, placing the centrifuged vacuum blood collection tube 3 into a gelling apparatus 4 and heating it to make it gel;

[0041] Step 4, after the vacuum blood collection tube 3 is heated and cooled, remove the rubber stopper 2, take a needle-free syringe 1, insert the cone of the needle-free syringe 1 into the hole in the middle of the piston 5 in the tube, and make the cone on the needle-free syringe 1 fit tightly with the fitting groove 7, then hold the curled edge of the outer jacket of the needle-free syringe 1 and push the piston 5 downward, first remove the air in the vacuum blood collection tube 3 and then extract the PRF gel into the needle-free syringe 1.

[0042] In this embodiment, the preparation process of the liquid injectable PRF gel is as follows:

[0043] Step 1: Use a blood collection needle to perform venipuncture, and then insert the needle tube at the other end of the blood collection needle into the vacuum blood collection tube 3, and the required volume of blood is automatically collected due to the vacuum in the tube;

[0044] Step 2: Place the vacuum blood collection tube 3 after collecting blood into the centrifuge. If necessary, take a balance tube and place it face to face with the vacuum blood collection tube 3 into the centrifuge to ensure that the centrifuge remains balanced. Centrifuge after balancing.

[0045] Step 3: After the vacuum blood collection tube 3 is centrifuged and taken out, remove the rubber stopper 2, take a needle-free syringe 1, insert the cone of the needle-free syringe 1 into the liquid extraction hole 6 in the middle of the piston 5 in the tube, hold the curled edge of the outer jacket of the needle-free syringe 1 and push the piston 5 downward, first extract the air and the top part of the liquid in the vacuum blood collection tube 3, and then extract the injectable PRF with a higher concentration of platelets and growth factors into the needle-free syringe 1.

[0046] In this embodiment, the temperature of the gel in step 3 is 75-90° C. and the gel time is 10-15 min, which can effectively ensure the quality of the injectable PRF gel.

[0047] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for preparing platelet-rich fibrin, characterized in that: The invention comprises a vacuum blood collection tube (3), wherein a rubber plug (2) is installed at the top of the vacuum blood collection tube (3), a piston (5) is installed in the vacuum blood collection tube (3) below the rubber plug (2), a needle-free syringe (1) is arranged above the vacuum blood collection tube (3), and a gelator (4) is arranged below the vacuum blood collection tube (3).

2. A device for preparing platelet-rich fibrin according to claim 1, characterized in that: The rubber plug (2) is plugged into the vacuum blood collection tube (3), and the rubber plug (2) is a medical rubber plug.

3. The device for preparing platelet-rich fibrin according to claim 1, characterized in that: The piston (5) is composed of a plug body and a sealing ring on the outside of the plug body after installation, and a liquid extraction hole (6) is provided in the middle of the piston (5), and fitting grooves (7) are provided at both the upper and lower ends of the piston (5).

4. The device for preparing platelet-rich fibrin according to claim 3, characterized in that: The liquid extraction hole (6) passes through the piston (5), and the fitting groove (7) is a conical groove structure. The size of the fitting groove (7) matches the size of the cone head at the bottom end of the needle-free syringe (1).

5. The device for preparing platelet-rich fibrin according to claim 1, characterized in that: The gelling apparatus (4) is provided with a slot for placing the vacuum blood collection tube (3), and the gelling apparatus (4) is an intelligent temperature-controlled gelling device.

6. The device for preparing platelet-rich fibrin according to claim 1, characterized in that: The preparation process of injectable PRF gel is as follows: Step 1: Use one end of a blood collection needle to perform venipuncture on the patient, and then insert the needle tube at the other end of the blood collection needle into a vacuum blood collection tube (3), and the required volume of blood is automatically collected due to the vacuum in the tube; Step 2, placing the vacuum blood collection tube (3) after collecting blood into the centrifuge, and if necessary, taking a balance tube and placing it face to face with the vacuum blood collection tube (3) into the centrifuge to ensure that the centrifuge remains balanced, and centrifuging after balancing; Step 3, placing the centrifuged vacuum blood collection tube (3) into a gelling apparatus (4) and heating it to make it gel; Step 4: After the vacuum blood collection tube (3) is heated and cooled, remove the rubber stopper (2), take a needle-free syringe (1), insert the cone of the needle-free syringe (1) into the hole in the middle of the piston (5) in the tube, and make the cone on the needle-free syringe (1) fit tightly with the fitting groove (7), then hold the curled edge of the outer jacket of the needle-free syringe (1) and push the piston (5) downward, first remove the air in the vacuum blood collection tube (3) and then extract the PRF gel into the needle-free syringe (1).

7. The device for preparing platelet-rich fibrin according to claim 1, characterized in that: The preparation process of liquid injectable PRF gel is as follows: Step 1: Use a blood collection needle to perform venipuncture, and then insert the needle tube at the other end of the blood collection needle into the vacuum blood collection tube (3). Due to the vacuum in the tube, the required volume of blood is automatically collected; Step 2: Place the vacuum blood collection tube (3) after collecting blood into the centrifuge. If necessary, take a balance tube and place it face to face with the vacuum blood collection tube (3) into the centrifuge to ensure that the centrifuge remains balanced. Centrifuge after balancing. Step 3: After the vacuum blood collection tube (3) is centrifuged and taken out, remove the rubber stopper (2), take a needle-free syringe (1), insert the cone of the needle-free syringe (1) into the liquid extraction hole (6) in the middle of the piston (5) in the tube, hold the curled edge of the outer jacket of the needle-free syringe (1) and push the piston (5) downward to first extract the air and the top layer of liquid in the vacuum blood collection tube (3), and then extract the injectable PRF with a higher concentration of platelets and growth factors into the needle-free syringe (1).

8. The device for preparing platelet-rich fibrin according to claim 6, characterized in that: The temperature of the gel in step 3 is 75-90° C., and the gel time is 10-15 min.

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