Application of complex of trace elements in feed additive

Abstract

The invention discloses application of a microelement complex in a feed additive, which comprises the following steps: mixing fresh kelp and water with the same mass as the fresh kelp, grinding and pulping to obtain kelp slurry, then adding compound enzyme into the kelp slurry, stirring and mixing uniformly, continuously adding boric acid and ammonium molybdate tetrahydrate under a magnetic field condition, stirring and mixing uniformly, and carrying out enzymolysis and chelation under an ultrasonic condition; sequentially adding zinc sulfate heptahydrate, ferrous sulfate heptahydrate, copper sulfate pentahydrate, cobaltous sulfate heptahydrate and manganese sulfate tetrahydrate while stirring, carrying out chelation reaction under a magnetic field condition, and centrifuging to take supernatant, so as to obtain a seaweed chelating solution; then adding maltose into the seaweed chelating liquid, uniformly stirring and mixing, continuously adding sodium selenite, and carrying out chelation reaction under the condition of a high-voltage pulsed electric field; and finally, continuously adding a beta-calcium polymalate aqueous solution under a magnetic field condition, carrying out ultrasonic oscillation treatment, and carrying out spray drying to obtain a complex of trace elements. The obtained complex of the trace elements can be used for preparing a feed additive, raw material sources are safe, and the complex has an obvious promoting effect on animal growth.

Description

Technical Field

[0001] The invention relates to the application of complexes of trace elements in feed additives, belonging to the technical field of feed processing. Background Art

[0002] Iron (Fe), copper (Cu), manganese (Mn), zinc (Zn), etc. are essential trace elements for animal growth and play an important role in the animal body. Lack of trace elements will affect the normal growth of animals and reduce their production performance. Due to the large differences in the content and potency of trace elements in feed, trace elements are usually supplemented in the form of feed additives in animal breeding.

[0003] The most common trace elements in animal husbandry are inorganic trace elements, which have low bioavailability and the unused parts are excreted from the body, causing waste and increasing the burden on the animal body and the environment. At present, amino acids such as lysine, methionine, and glycine are used to chelate trace elements, which have high biological efficacy, but the cost of use is also relatively high, which limits their promotion and use.

[0004] In addition, in production practice, the absorption rate of organic trace elements has not yet reached the ideal state. Therefore, it is very necessary to study the complexation of trace elements.

[0005] Patent CN101653199B discloses a water-soluble feed additive glycine iron complex, which is obtained by complexing glycine and ferrous sulfate as raw materials. The raw material glycine of this patented technology uses industrial-grade raw materials in production practice, which contains a large amount of carcinogens such as dioxins, posing a safety hazard. Summary of the invention

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide an application of a complex of trace elements in feed additives.

[0007] To achieve the above object, the present invention adopts the following technical solutions:

[0008] The application of the complex of trace elements in feed additives, measured by weight, is prepared by the following method:

[0009] (1) firstly, 10 parts of fresh kelp and an equal amount of water are mixed, ground and pulped to obtain kelp slurry, then 0.2-0.3 parts of complex enzyme are added to the kelp slurry, stirred and mixed, 0.1-0.2 parts of boric acid and 0.1-0.2 parts of ammonium molybdate tetrahydrate are added under magnetic field conditions, stirred and mixed, and enzymatically hydrolyzed and chelated under ultrasonic conditions;

[0010] (2) adding 0.4-0.5 parts of zinc sulfate heptahydrate, 0.6-0.8 parts of ferrous sulfate heptahydrate, 0.2-0.3 parts of copper sulfate pentahydrate, 0.2-0.3 parts of cobalt sulfate heptahydrate, and 0.2-0.3 parts of manganese sulfate tetrahydrate in sequence while stirring, carrying out chelation reaction under magnetic field conditions, and centrifuging to obtain supernatant to obtain seaweed liquid chelate;

[0011] (3) then adding 1-2 parts of maltose to the seaweed liquid chelate solution, stirring and mixing, and then adding 0.1-0.2 parts of sodium selenite to carry out a chelation reaction under high voltage pulse electric field conditions;

[0012] (4) Finally, 4 to 5 parts of β-polymalate calcium aqueous solution with a mass concentration of 15 to 25% are added under magnetic field conditions, ultrasonic oscillation treatment is performed, and spray drying is performed to obtain the product.

[0013] Preferably, in step (1), the complex enzyme is obtained by mixing alginate lyase, cellulase and pectinase in a mass ratio of 3-4:0.3-0.4:1-2.

[0014] Preferably, in step (1), the magnetic field strength is 8 to 10 A / m.

[0015] Preferably, in step (1), the magnetic field strength is kept constant during enzymatic hydrolysis and chelation, and the enzymatic hydrolysis and chelation are carried out at 40 to 42° C. for 4 to 5 hours under the conditions of a frequency of 20 to 30 kHz and 400 to 500 W ultrasonic oscillation.

[0016] Preferably, in step (2), the magnetic field strength is 12 to 15 A / m.

[0017] Preferably, in step (2), the chelating reaction temperature is 42-45° C. and the reaction time is 4-5 hours.

[0018] Preferably, in step (3), the high voltage pulse electric field conditions are: electric field strength 8-10 kV / cm, pulse number 30-40 times, pulse width 100-200 μs.

[0019] Preferably, in step (3), the chelating reaction temperature is 40-45° C. and the reaction time is 5-7 minutes.

[0020] Preferably, in step (4), the β-polycalcium malate aqueous solution is obtained by removing fermentation cells and planoside from β-polycalcium malate fermentation broth from a process for preparing polymalic acid by conventional microbial fermentation by using plate and frame filtration, ultrafiltration or the like; the β-polycalcium malate contained therein has a degree of polymerization of 35 to 40 and a calcium content of 10 to 15%.

[0021] Preferably, in step (4), the magnetic field strength is 12 to 15 A / m.

[0022] Preferably, in step (4), the magnetic field intensity is kept constant during the ultrasonic oscillation treatment, and the ultrasonic oscillation treatment is performed at a frequency of 20 to 30 kHz and 400 to 500 W for 50 to 60 minutes.

[0023] Beneficial effects of the present invention:

[0024] The present invention first mixes fresh kelp with water of equal mass, grinds and pulps to obtain kelp slurry, then adds complex enzyme to the kelp slurry, stirs and mixes, continues to add boric acid and ammonium molybdate tetrahydrate under magnetic field conditions, stirs and mixes, and performs enzymolysis and chelation under ultrasonic conditions; adds zinc sulfate heptahydrate, ferrous sulfate heptahydrate, copper sulfate pentahydrate, cobalt sulfate heptahydrate, and manganese sulfate tetrahydrate in sequence while stirring, performs chelation reaction under magnetic field conditions, centrifuges and takes supernatant to obtain seaweed liquid chelate; then adds maltose to the seaweed liquid chelate, stirs and mixes, continues to add sodium selenite, performs chelation reaction under high-voltage pulse electric field conditions; finally, continues to add β-polymalate calcium aqueous solution under magnetic field conditions, performs ultrasonic oscillation treatment, and sprays and dries to obtain a complex of trace elements. The complex of trace elements obtained by the present invention can be used for the preparation of feed additives, the source of raw materials is safe, and it has a significant promoting effect on animal growth.

[0025] The present invention introduces trace elements such as boron, molybdenum, zinc, iron, copper, cobalt, manganese, selenium, calcium, etc., which can provide richer nutritional support for animal growth, thereby promoting animal growth.

[0026] The present invention mainly uses small peptides, polysaccharides, oligosaccharides, mannitol and the like in the kelp enzymatic hydrolysate to better chelate the aforementioned trace elements, thereby improving the biological efficacy of each trace element, having stable chemical properties, good palatability, and significantly promoting animal growth. In addition, calcium is introduced in the form of β-polycalcium malate, which also has the aforementioned advantages and is also beneficial to improving the gastrointestinal microenvironment of animals, further promoting animal growth.

[0027] In addition, the present invention ensures the chelating effect through technical means such as magnetic field treatment, high-voltage pulse electric field treatment, ultrasonic oscillation treatment, or the coupling effect between them, and the obtained product has a better promoting effect on animal growth. DETAILED DESCRIPTION

[0028] The present invention is further described below in conjunction with embodiments. It should be noted that the following description is only for explaining the present invention and does not limit its contents.

[0029] Embodiment 1:

[0030] The complex of trace elements is prepared by the following method:

[0031] (1) First, 10 kg of fresh kelp and an equal amount of water are mixed, ground and pulped to obtain kelp slurry, then 0.2 kg of complex enzyme is added to the kelp slurry, stirred and mixed, 0.1 kg of boric acid and 0.1 kg of ammonium molybdate tetrahydrate are added under magnetic field conditions, stirred and mixed, and enzymatically hydrolyzed and chelated under ultrasonic conditions;

[0032] The complex enzyme is obtained by mixing alginate lyase, cellulase and pectinase in a mass ratio of 3:0.3:1.

[0033] The magnetic field strength is 8A / m.

[0034] The magnetic field strength was kept constant during the enzymatic hydrolysis and chelation, and the enzymatic hydrolysis and chelation were carried out at 40° C. for 4 hours under the conditions of a frequency of 20 kHz and 400 W ultrasonic oscillation.

[0035] (2) adding 0.4 kg of zinc sulfate heptahydrate, 0.6 kg of ferrous sulfate heptahydrate, 0.2 kg of copper sulfate pentahydrate, 0.2 kg of cobalt sulfate heptahydrate, and 0.2 kg of manganese sulfate tetrahydrate in sequence while stirring, carrying out a chelation reaction under magnetic field conditions, and centrifuging to obtain a supernatant to obtain a seaweed liquid chelate;

[0036] The magnetic field strength is 12A / m.

[0037] The chelation reaction temperature was 42°C and the reaction time was 4 hours.

[0038] (3) then adding 1 kg of maltose to the seaweed liquid chelate solution, stirring and mixing, and then adding 0.1 kg of sodium selenite to carry out a chelation reaction under high voltage pulse electric field conditions;

[0039] The high-voltage pulse electric field conditions were as follows: electric field intensity 8 kV / cm, pulse number 30 times, and pulse width 100 μs.

[0040] The chelation reaction temperature was 40°C and the reaction time was 5 minutes.

[0041] (4) Finally, 4 kg of 15% β-polymalate calcium aqueous solution was added under magnetic field conditions, ultrasonic oscillation treatment was performed, and spray drying was performed to obtain the product.

[0042] The β-polymalate calcium aqueous solution is obtained by removing fermentation cells and planoside from β-polymalate calcium fermentation liquid from a conventional microbial fermentation process for preparing polymalic acid by plate and frame filtration, ultrafiltration and the like; the β-polymalate calcium aqueous solution has a polymerization degree of 40 and a calcium content of 12%.

[0043] The magnetic field strength is 12A / m.

[0044] The magnetic field intensity was kept constant during the ultrasonic oscillation treatment, and the ultrasonic oscillation treatment was performed at a frequency of 20 kHz and 400 W for 50 minutes.

[0045] Embodiment 2:

[0046] The complex of trace elements is prepared by the following method:

[0047] (1) First, 10 kg of fresh kelp and an equal amount of water are mixed, ground and pulped to obtain kelp slurry, then 0.3 kg of complex enzyme is added to the kelp slurry, stirred and mixed, 0.2 kg of boric acid and 0.2 kg of ammonium molybdate tetrahydrate are added under magnetic field conditions, stirred and mixed, and enzymatically hydrolyzed and chelated under ultrasonic conditions;

[0048] The complex enzyme is obtained by mixing alginate lyase, cellulase and pectinase in a mass ratio of 4:0.4:2.

[0049] The magnetic field strength is 10A / m.

[0050] The magnetic field strength was kept constant during the enzymatic hydrolysis and chelation, and the enzymatic hydrolysis and chelation were carried out at 42° C. for 5 hours under the conditions of a frequency of 30 kHz and 500 W ultrasonic oscillation.

[0051] (2) adding 0.5 kg of zinc sulfate heptahydrate, 0.8 kg of ferrous sulfate heptahydrate, 0.3 kg of copper sulfate pentahydrate, 0.3 kg of cobalt sulfate heptahydrate, and 0.3 kg of manganese sulfate tetrahydrate in sequence while stirring, carrying out a chelation reaction under magnetic field conditions, and centrifuging to obtain a supernatant to obtain a seaweed liquid chelate;

[0052] The magnetic field strength is 15A / m.

[0053] The chelation reaction was carried out at 45°C for 5 hours.

[0054] (3) then adding 2 kg of maltose to the seaweed liquid chelate solution, stirring and mixing, and then adding 0.2 kg of sodium selenite to carry out a chelation reaction under high voltage pulse electric field conditions;

[0055] The high voltage pulse electric field conditions are: electric field intensity 10 kV / cm, pulse number 40 times, pulse width 200 μs.

[0056] The chelation reaction temperature was 45°C and the reaction time was 7 minutes.

[0057] (4) Finally, 5 kg of 25% β-polymalate calcium aqueous solution was added under magnetic field conditions, ultrasonic oscillation treatment was performed, and spray drying was performed to obtain the product.

[0058] The β-polymalate calcium aqueous solution is obtained by removing fermentation cells and planoside from β-polymalate calcium fermentation liquid from a conventional microbial fermentation process for preparing polymalic acid by plate and frame filtration, ultrafiltration and the like; the β-polymalate calcium aqueous solution has a polymerization degree of 40 and a calcium content of 12%.

[0059] The magnetic field strength is 15A / m.

[0060] The magnetic field intensity was kept constant during the ultrasonic oscillation treatment, and the ultrasonic oscillation treatment was performed at a frequency of 30 kHz and 500 W for 60 minutes.

[0061] Embodiment 3:

[0062] The complex of trace elements is prepared by the following method:

[0063] (1) First, 10 kg of fresh kelp and an equal amount of water are mixed, ground and pulped to obtain kelp slurry, then 0.2 kg of complex enzyme is added to the kelp slurry, stirred and mixed, 0.2 kg of boric acid and 0.1 kg of ammonium molybdate tetrahydrate are added under magnetic field conditions, stirred and mixed, and enzymatically hydrolyzed and chelated under ultrasonic conditions;

[0064] The complex enzyme is obtained by mixing alginate lyase, cellulase and pectinase in a mass ratio of 4:0.3:2.

[0065] The magnetic field strength is 8A / m.

[0066] The magnetic field strength was kept constant during the enzymatic hydrolysis and chelation, and the enzymatic hydrolysis and chelation were carried out at 42° C. for 4 hours under the conditions of 30 kHz frequency and 400 W ultrasonic oscillation.

[0067] (2) adding 0.5 kg of zinc sulfate heptahydrate, 0.6 kg of ferrous sulfate heptahydrate, 0.3 kg of copper sulfate pentahydrate, 0.2 kg of cobalt sulfate heptahydrate, and 0.3 kg of manganese sulfate tetrahydrate in sequence while stirring, carrying out chelation reaction under magnetic field conditions, and centrifuging to obtain the supernatant to obtain a seaweed liquid chelate;

[0068] The magnetic field strength is 12A / m.

[0069] The chelation reaction was carried out at 45°C for 4 hours.

[0070] (3) then adding 2 kg of maltose to the seaweed liquid chelate solution, stirring and mixing, and then adding 0.1 kg of sodium selenite to carry out a chelation reaction under high voltage pulse electric field conditions;

[0071] The high-voltage pulse electric field conditions were as follows: electric field intensity 10 kV / cm, pulse number 30 times, and pulse width 200 μs.

[0072] The chelation reaction temperature was 40°C and the reaction time was 7 minutes.

[0073] (4) Finally, 4 kg of 25% β-polymalate calcium aqueous solution was added under magnetic field conditions, ultrasonic oscillation treatment was performed, and spray drying was performed to obtain the product.

[0074] The β-polymalate calcium aqueous solution is obtained by removing fermentation cells and planoside from β-polymalate calcium fermentation liquid from a conventional microbial fermentation process for preparing polymalic acid by plate and frame filtration, ultrafiltration and the like; the β-polymalate calcium aqueous solution has a polymerization degree of 40 and a calcium content of 12%.

[0075] The magnetic field strength is 12A / m.

[0076] The magnetic field intensity was kept constant during the ultrasonic oscillation treatment, and the ultrasonic oscillation treatment was performed at a frequency of 30 kHz and 400 W for 60 minutes.

[0077] Embodiment 4:

[0078] The application of the complex of trace elements in feed additives, wherein the complex of trace elements is prepared by the following method:

[0079] (1) First, 10 kg of fresh kelp and an equal amount of water are mixed, ground and pulped to obtain kelp slurry, then 0.25 kg of complex enzyme is added to the kelp slurry, stirred and mixed, 0.15 kg of boric acid and 0.15 kg of ammonium molybdate tetrahydrate are added under magnetic field conditions, stirred and mixed, and enzymatically hydrolyzed and chelated under ultrasonic conditions;

[0080] The complex enzyme is obtained by mixing alginate lyase, cellulase and pectinase in a mass ratio of 3.5:0.35:1.5.

[0081] The magnetic field strength is 9A / m.

[0082] The magnetic field strength was kept constant during the enzymatic hydrolysis and chelation, and the enzymatic hydrolysis and chelation were carried out at 41° C. for 4.5 hours under the conditions of a frequency of 25 kHz and 500 W ultrasonic oscillation.

[0083] (2) adding 0.45 kg of zinc sulfate heptahydrate, 0.7 kg of ferrous sulfate heptahydrate, 0.25 kg of copper sulfate pentahydrate, 0.25 kg of cobalt sulfate heptahydrate, and 0.25 kg of manganese sulfate tetrahydrate in sequence while stirring, carrying out chelation reaction under magnetic field conditions, and centrifuging to obtain the supernatant to obtain a seaweed liquid chelate;

[0084] The magnetic field strength is 13A / m.

[0085] The chelation reaction temperature was 43°C and the reaction time was 4.5 hours.

[0086] (3) then adding 1.5 kg of maltose to the seaweed liquid chelate solution, stirring and mixing, and then adding 0.15 kg of sodium selenite to carry out a chelation reaction under high voltage pulse electric field conditions;

[0087] The high voltage pulse electric field conditions are: electric field intensity 9 kV / cm, pulse number 35 times, pulse width 200 μs.

[0088] The chelation reaction temperature was 42°C and the reaction time was 6 minutes.

[0089] (4) Finally, 4.5 kg of 20% β-polymalate calcium aqueous solution was added under magnetic field conditions, ultrasonic oscillation treatment was performed, and spray drying was performed to obtain the product.

[0090] The β-polymalate calcium aqueous solution is obtained by removing fermentation cells and planoside from β-polymalate calcium fermentation liquid from a conventional microbial fermentation process for preparing polymalic acid by plate and frame filtration, ultrafiltration and the like; the β-polymalate calcium aqueous solution has a polymerization degree of 40 and a calcium content of 12%.

[0091] The magnetic field strength is 13A / m.

[0092] The magnetic field intensity was kept constant during the ultrasonic oscillation treatment, and the ultrasonic oscillation treatment was performed at a frequency of 25 kHz and 500 W for 55 minutes.

[0093] Comparative Example

[0094] The complex of trace elements is prepared by the following method:

[0095] (1) First, 10 kg of fresh kelp and an equal amount of water are mixed, ground and pulped to obtain kelp slurry, then 0.2 kg of complex enzyme is added to the kelp slurry, stirred and mixed, and then 0.1 kg of boric acid and 0.1 kg of ammonium molybdate tetrahydrate are added, stirred and mixed, and enzymatic hydrolysis and chelation are performed under ultrasonic conditions;

[0096] The complex enzyme is obtained by mixing alginate lyase, cellulase and pectinase in a mass ratio of 3:0.3:1.

[0097] The enzymatic hydrolysis and chelation conditions are: enzymatic hydrolysis and chelation at 40° C. for 4 hours under the conditions of 20 kHz frequency and 400 W ultrasonic oscillation.

[0098] (2) adding 0.4 kg of zinc sulfate heptahydrate, 0.6 kg of ferrous sulfate heptahydrate, 0.2 kg of copper sulfate pentahydrate, 0.2 kg of cobalt sulfate heptahydrate, and 0.2 kg of manganese sulfate tetrahydrate in sequence while stirring to carry out chelation reaction, and centrifuging to obtain the supernatant to obtain a seaweed liquid chelate;

[0099] The chelation reaction temperature was 42°C and the reaction time was 4 hours.

[0100] (3) then adding 1 kg of maltose to the seaweed liquid chelate solution, stirring and mixing, and then adding 0.1 kg of sodium selenite to carry out a chelation reaction under high voltage pulse electric field conditions;

[0101] The high-voltage pulse electric field conditions were as follows: electric field intensity 8 kV / cm, pulse number 30 times, and pulse width 100 μs.

[0102] The chelation reaction temperature was 40°C and the reaction time was 5 minutes.

[0103] (4) Continue to add 4 kg of 15% mass concentration β-polymalate calcium aqueous solution, treat with ultrasonic oscillation, and spray dry to obtain.

[0104] The β-polymalate calcium aqueous solution is obtained by removing fermentation cells and planoside from β-polymalate calcium fermentation liquid from a conventional microbial fermentation process for preparing polymalic acid by plate and frame filtration, ultrafiltration and the like; the β-polymalate calcium aqueous solution has a polymerization degree of 40 and a calcium content of 12%.

[0105] The ultrasonic oscillation treatment conditions are: a frequency of 20 kHz and 400 W for 50 minutes.

[0106] Test example

[0107] The complexes of trace elements obtained in Examples 1 to 4 and the comparative example were used to carry out animal feeding experiments, and the specific methods are as follows:

[0108] 50 weaned piglets weighing about 8kg were randomly divided into 5 groups, and the complexes of trace elements obtained in Examples 1 to 4 and the comparative example were used as feed additives to match the basic diet, specifically the basic diet + 200 mg / kg of the complex of trace elements, and the basic diet composition was: 53kg corn, 19kg peeled soybean meal, 9kg puffed soybean, 5kg fish meal, 5kg whey powder, 3kg glucose, 2kg oil, 0.8kg stone powder, 1kg calcium hydrogen phosphate, and 0.3kg sodium chloride. Free feeding, free drinking water, and continuous feeding for 30 days, the average weight gain and feed-to-meat ratio of each piglet were calculated, and the results are shown in Table 1.

[0109] Table 1. Results of animal feeding experiments

[0110] Average weight gain (kg) Feed to meat ratio Example 1 13.5 1.32 Example 2 13.8 1.32 Example 3 14.2 1.26 Example 4 14.9 1.25 Comparative Example 11.6 1.89

[0111] It can be seen from Table 1 that the complexes of trace elements obtained in Examples 1 to 4 can significantly promote the growth of piglets with a low feed-to-meat ratio.

[0112] In the control group, the magnetic field treatment was omitted, and the promoting effect of the trace element complex on the growth of piglets was significantly weakened, and the feed-to-meat ratio became higher, indicating that the magnetic field treatment, high-voltage pulse electric field treatment, ultrasonic oscillation treatment and other treatment methods of the present application are coupled and synergistic, promoting the full chelation of trace elements and improving the promoting effect of the product on the growth of piglets.

[0113] Although the above describes the specific implementation methods of the present invention, it does not limit the scope of protection of the present invention. Based on the technical solution of the present invention, various modifications or variations that can be made by those skilled in the art without creative work are still within the scope of protection of the present invention.

Claims

1. The application of the complex of trace elements in feed additives is characterized by: In parts by weight, the complex of trace elements is prepared by the following method: (1) firstly, 10 parts of fresh kelp and an equal amount of water are mixed, ground and pulped to obtain kelp slurry, then 0.2-0.3 parts of complex enzyme are added to the kelp slurry, stirred and mixed, 0.1-0.2 parts of boric acid and 0.1-0.2 parts of ammonium molybdate tetrahydrate are added under magnetic field conditions, stirred and mixed, and enzymatically hydrolyzed and chelated under ultrasonic conditions; (2) adding 0.4-0.5 parts of zinc sulfate heptahydrate, 0.6-0.8 parts of ferrous sulfate heptahydrate, 0.2-0.3 parts of copper sulfate pentahydrate, 0.2-0.3 parts of cobalt sulfate heptahydrate, and 0.2-0.3 parts of manganese sulfate tetrahydrate in sequence while stirring, carrying out chelation reaction under magnetic field conditions, and centrifuging to obtain supernatant to obtain seaweed liquid chelate; (3) then adding 1-2 parts of maltose to the seaweed liquid chelate solution, stirring and mixing, and then adding 0.1-0.2 parts of sodium selenite to carry out a chelation reaction under high voltage pulse electric field conditions; (4) Finally, 4 to 5 parts of β-polymalate calcium aqueous solution with a mass concentration of 15 to 25% are added under magnetic field conditions, ultrasonic oscillation treatment is performed, and spray drying is performed to obtain the product.

2. The use according to claim 1, characterized in that: In step (1), the complex enzyme is obtained by mixing alginate lyase, cellulase and pectinase in a mass ratio of 3-4:0.3-0.4:1-2.

3. The use according to claim 1, characterized in that: In step (1), the magnetic field strength is 8 to 10 A / m.

4. The use according to claim 1, characterized in that: In step (1), the magnetic field strength is kept constant during enzymatic hydrolysis and chelation, and the enzymatic hydrolysis and chelation are carried out at 40 to 42° C. for 4 to 5 hours under the conditions of a frequency of 20 to 30 kHz and 400 to 500 W ultrasonic oscillation.

5. The use according to claim 1, characterized in that: In step (2), the magnetic field strength is 12 to 15 A / m.

6. The use according to claim 1, characterized in that: In step (2), the chelating reaction is carried out at a temperature of 42 to 45° C. and for a time of 4 to 5 hours.

7. The use according to claim 1, characterized in that: In step (3), the high voltage pulse electric field conditions are: electric field strength 8-10 kV / cm, pulse number 30-40 times, pulse width 100-200 μs.

8. The use according to claim 1, characterized in that: In step (3), the chelating reaction temperature is 40-45° C. and the reaction time is 5-7 minutes.

9. The use according to claim 1, characterized in that: In step (4), the magnetic field strength is 12 to 15 A / m.

10. The use according to claim 1, characterized in that: In step (4), the magnetic field intensity is kept constant during the ultrasonic oscillation treatment, and the ultrasonic oscillation treatment is performed at a frequency of 20 to 30 kHz and 400 to 500 W for 50 to 60 minutes.