Photosensitive imaging method

Abstract

The invention provides a photosensitive imaging method, which comprises the following steps of: opening a bin door (22) of an automatic bin entering and exiting device (11) of a photosensitive imager (10), and exposing a photosensitive chip assembly (65); a sample (70) is placed on the photosensitive chip assembly (65), and then the bin door (22) is closed; lightening the whole backlight assembly (66) to irradiate the photosensitive chip assembly (65) and the sample (70) on the photosensitive chip assembly (65); the photosensitive chip assembly (65) collects an image and identifies the position of the sample (70), and determines a sample area (71) and a non-sample area (72); sequentially lighting different colors of light of the local backlight assembly (66) so that only the sample area (71) is provided with backlight and the non-sample area (72) is not provided with backlight; the signal values of the backlight samples (70) of different colors are obtained through the photosensitive chip assembly (65), and a color image of the samples (70) is synthesized in combination with a software algorithm. According to the photosensitive imaging method, the signal-to-noise ratio of the image can be increased, the color image is obtained, and simultaneous detection of multiple samples is supported.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering or medical device technology, and specifically to a photosensitive imaging method, in particular to a back-illuminated structured light projection imaging technology using a photosensitive imager. Background Art

[0002] Photosensitive imagers are mainly used for protein and nucleic acid gel imaging and analysis. Usually, they provide white light, ultraviolet light and blue light sources for photographing gels, and use the system's built-in image capture software to capture images, and then use image analysis software to analyze the captured images. This device can automatically find gel bands and has multiple functions such as molecular weight quantification, density quantification, PCR quantification, etc. It is suitable for qualitative analysis of the separation and purification results of biological molecules such as DNA, RNA, and proteins.

[0003] Existing photosensitive imagers usually use an integral backlight, that is, the backlight component emits light as a whole, such as a whole backlight panel emits light as a whole, and the light-emitting area cannot be partitioned or arbitrarily controlled. In this way, it provides an overall backlight to the detected area when working, and the entire detected area is illuminated with a uniform lighting color. Existing photosensitive imagers cannot provide background light for a specific target area (such as a sample area) separately, and the image signal-to-noise ratio obtained is relatively low, and the target detection signal is not prominent. In addition, existing photosensitive imagers and back-illuminated structured light projection imaging technology can only generate black and white images. Summary of the invention

[0004] The purpose of the present invention is to at least partially overcome the defects of the prior art and provide a novel photosensitive imaging method.

[0005] Another object of the present invention is to provide a photosensitive imaging method capable of increasing the image signal-to-noise ratio.

[0006] Another object of the present invention is to provide a photosensitive imaging method capable of obtaining a color image.

[0007] The present invention also aims to provide a photosensitive imaging method that supports simultaneous detection of multiple samples.

[0008] To achieve the above purpose or one of the purposes, the technical solution of the present invention is as follows:

[0009] A photosensitive imaging method, the photosensitive imaging method comprising:

[0010] Step S1: Open the door of the automatic entry and exit device of the photosensitive imager to expose the photosensitive chip assembly;

[0011] Step S2: placing the sample on the photosensitive chip assembly and then closing the chamber door;

[0012] Step S3: Light up the entire backlight assembly to illuminate the photosensitive chip assembly and the sample on the photosensitive chip assembly;

[0013] Step S4: the photosensitive chip component collects images and identifies the location of the sample, and determines the sample area and the non-sample area;

[0014] Step S5: lighting up lights of different colors of the local backlight assembly in sequence, so that only the sample area is provided with backlight, and the non-sample area is not provided with backlight;

[0015] Step S6: Acquire the signal values ​​of samples with different color backlights through the photosensitive chip component, and synthesize the color image of the sample in combination with the software algorithm.

[0016] According to a preferred embodiment of the present invention, the photosensitive imaging method further comprises:

[0017] Changes the color of the backlight provided by the backlight assembly.

[0018] According to a preferred embodiment of the present invention, the photosensitive chip assembly is a light intensity detection chip.

[0019] According to a preferred embodiment of the present invention, the photosensitive imaging method forms a color image by using different color backlights and light intensity detection chips through a software algorithm.

[0020] According to a preferred embodiment of the present invention, multiple samples are tested simultaneously.

[0021] According to a preferred embodiment of the present invention, the photosensitive imaging method uses a photosensitive imager, and the photosensitive imager includes:

[0022] The automatic in-and-out storage device;

[0023] The photosensitive chip assembly is arranged on the automatic storage device and is configured to be able to move with the automatic storage device between a first position located inside the photosensitive imager and a second position extending outside the photosensitive imager; and

[0024] The backlight assembly is arranged inside the photosensitive imager and faces the photosensitive chip assembly at the first position.

[0025] According to a preferred embodiment of the present invention, the photosensitive imager further comprises:

[0026] The system control component is fixed on the fixed base and the backlight component, and is configured to convert the external power supply into the power required by the photosensitive imager, and control the in-and-out operation of the automatic in-and-out device and the operation of the backlight component, and provide an input and output interface and a wireless connection interface to transmit data;

[0027] A central control component, fixed on the system control component, for providing human-computer interaction services to users; and

[0028] The photosensitive chip control component is configured to adjust and control the photosensitive chip component to obtain sample luminescence data.

[0029] According to a preferred embodiment of the present invention, at least one layer of coating is provided on the surface of the photosensitive chip assembly facing the backlight assembly, and the coating is used to contact with and carry the sample.

[0030] According to a preferred embodiment of the present invention, an indicator light and a control switch are provided on the cover of the photosensitive imager, the indicator light is used to indicate the working status of the photosensitive imager, and the control switch is used to control the opening and closing of the automatic in-and-out warehouse device.

[0031] According to a preferred embodiment of the present invention, the automatic warehouse entry and exit device comprises:

[0032] The fixed base;

[0033] a frame configured to be movable along a fixed base;

[0034] A barn door, which is arranged on the frame;

[0035] A drive assembly, wherein the drive assembly is fixedly disposed on a fixed base; and

[0036] The transmission element is arranged between the driving assembly and the frame, and is used for transmitting the actuating force of the driving assembly to the frame.

[0037] The present invention belongs to a technology for detecting chemiluminescence in photosensitive imaging. The photosensitive imaging method of the present invention can provide background light only for the target area (sample area), so that the light difference between the sample area and the non-sample area is obvious, and the image signal-to-noise ratio is increased. This is because, if the background light is only provided for the sample area, then the periphery of the sample area is darker, and the image formed by the sample area is clearer. On the contrary, if the background light is provided for the entire area of ​​the photosensitive chip assembly, then the image is unclear, just like overexposure when taking a photo. In addition, the photosensitive imaging method of the present invention uses different color structured lights and light intensity detection chips to form a color image through a software algorithm, and the photosensitive chip assembly is large, can carry multiple samples, and supports simultaneous detection of multiple samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 is a three-dimensional diagram of a photosensitive imager according to an embodiment of the present invention, wherein the automatic in-and-out bin device is in a closed state;

[0039] Figure 2is a three-dimensional diagram of a photosensitive imager according to an embodiment of the present invention, wherein the automatic in-and-out bin device is in an open state;

[0040] Figure 3 is a side sectional view of a photosensitive imager according to an embodiment of the present invention;

[0041] Figure 4 is a three-dimensional diagram of a photosensitive imager according to an embodiment of the present invention, wherein the cover is removed and the automatic in-and-out bin device is in a closed state;

[0042] Figure 5 is a three-dimensional diagram of a photosensitive imager according to an embodiment of the present invention, wherein the cover is removed and the automatic in-and-out bin device is in an open state;

[0043] Figure 6 is a rear view of a photosensitive imager according to an embodiment of the present invention;

[0044] Figure 7 is a cross-sectional view of a photosensitive imager according to an embodiment of the present invention;

[0045] Figure 8 is a top cross-sectional view of a photosensitive imager according to an embodiment of the present invention;

[0046] Fig. 9 is a bottom cross-sectional view of a photosensitive imager according to an embodiment of the present invention;

[0047] Fig.10 is a three-dimensional diagram of an automatic warehouse entry and exit device according to an embodiment of the present invention, wherein the automatic warehouse entry and exit device is in a closed state;

[0048] Fig.11 Shown from another angle Fig.10 Automatic in and out warehouse device;

[0049] Fig.12 for Fig.10 A front view of an automatic in-and-out bin device;

[0050] Fig.13 for Fig.10 A top view of the automatic in-and-out warehouse device;

[0051] Fig.14 is a three-dimensional diagram of an automatic warehouse entry and exit device according to an embodiment of the present invention, wherein the automatic warehouse entry and exit device is in an open state;

[0052] Fig.15 Shown from another angle Fig.14 Automatic in and out warehouse device;

[0053] Fig.16 for Fig.14A top view of the automatic in-and-out warehouse device;

[0054] Fig.17 is a cross-sectional view of an automatic bin entry and exit device according to an embodiment of the present invention, wherein the automatic bin entry and exit device is in a closed state;

[0055] Fig.18 is another cross-sectional view of the automatic bin access device according to an embodiment of the present invention, wherein the automatic bin access device is in a closed state;

[0056] Fig.19 for Fig.18 A partial enlarged view of the automatic in-and-out warehouse device;

[0057] Fig. 20 An exploded view of a door, a frame, and a support plate of an automatic warehouse entry and exit device according to an embodiment of the present invention;

[0058] Fig.21 A three-dimensional diagram of a door fixing block of an automatic warehouse entry and exit device according to an embodiment of the present invention;

[0059] Fig. 22 4 is a flow chart of a photosensitive imaging method according to an embodiment of the present invention. DETAILED DESCRIPTION

[0060] Exemplary embodiments of the present invention are described in detail below in conjunction with the accompanying drawings, wherein the same or similar reference numerals represent the same or similar elements. In addition, in the detailed description below, for ease of explanation, many specific details are set forth to provide a comprehensive understanding of the disclosed embodiments. However, it is apparent that one or more embodiments may also be implemented without these specific details. In other cases, known structures and devices are embodied in a schematic manner to simplify the accompanying drawings.

[0061] Figure 1 , 2 A photosensitive imager 10 according to an embodiment of the present invention is shown. The photosensitive imager 10 has an automatic entry and exit device 11. A cover shell 12 is arranged on the upper side of the automatic entry and exit device 11. An indicator light 13 and a control switch 14 are arranged on the cover shell 12. The indicator light 13 is used to indicate the working status of the photosensitive imager 10, and the control switch 14 is used to control the opening and closing of the automatic entry and exit device 11.

[0062] Figure 3-9 The photosensitive imager 10 of the present invention is shown through a cross-sectional view, a partial stereoscopic view, etc., and the photosensitive imager 10 is mainly composed of an automatic in-and-out device 11, a photosensitive chip assembly 65, a backlight assembly 66, a system control assembly 62, a central control assembly 63, and a photosensitive chip control assembly 73. The automatic in-and-out device 11 is Figure 10-21In detail, the photosensitive chip assembly 65 is arranged on the automatic storage device 11, and is configured to be able to move with the automatic storage device 11 between a first position located inside the photosensitive imager 10 and a second position extending outside the photosensitive imager 10; the backlight assembly 66 is arranged inside the photosensitive imager 10, and faces the photosensitive chip assembly 65 in the first position. The backlight assembly can provide the system with backlights of different colors and brightness according to user needs. It should be noted that the distance between the photosensitive chip assembly 65 and the backlight assembly 66 is extremely short, less than 5 mm, preferably less than 1 mm, so as to achieve an extremely short light path.

[0063] like Figure 3-5 As shown, a U-shaped cover 61 is disposed in the housing 12 of the photosensitive imager 10, and the backlight assembly 66 is disposed on the inner side of the U-shaped cover 61; the photosensitive chip assembly 65 is disposed on the support plate 31. The system control assembly 62 is fixed on the fixed base 23 and the backlight assembly 66, and the fixing is performed by a support fixture 64. The system control assembly 62 is configured to convert an external power source into the power required by the photosensitive imager 10, and control the in-and-out operation of the automatic in-and-out device 11 and the operation of the backlight assembly 66, and at the same time provide an input and output interface and a wireless connection interface for data transmission; the central control assembly 63 is fixed on the system control assembly 62, as shown in FIG. Figure 5 As shown, the central control component 63 is used to provide human-computer interaction services for users; the photosensitive chip control component 73 is configured to adjust and control the photosensitive chip component 65 to obtain sample luminescence data.

[0064] Advantageously, at least one layer of coating is provided on the surface of the photosensitive chip assembly 65 facing the backlight assembly 66, and the coating is used to contact with and support the sample 70. The coating prevents the sample from contaminating the photosensitive chip assembly (detection chip) during the experiment, and the photosensitive chip assembly contacts the sample through the coating.

[0065] A power switch 68 and multiple transmission ports 69 are provided on the back side of the photosensitive imager 10. The optical path is extremely short, and the indicator light 13 (light bar assembly), the control switch 14 and the power switch 68 are fixed on the cover 12. The indicator light 13 can display the product status and the experimental progress. The indicator light 13 supplies power and signals through the wire 67. The control switch 14 can send the user's entry and exit requirements to the system control component to realize the entry and exit operation. The power switch 68 can realize the switch of the product.

[0066] The general working process of the photosensitive imager of the present invention is: the user connects the photosensitive imager to the specified power socket and turns it on, prepares the sample, opens the automatic entry and exit device, places the sample on the photosensitive chip of the automatic entry and exit device (to avoid bubbles), closes the automatic entry and exit device, connects the equipment through a display, pad, computer, etc., adjusts relevant parameters and settings as needed, and then obtains image data. Figure 8 It is schematically shown that a plurality of samples 70 are placed on the photosensitive chip assembly 65. When the backlight assembly 66 is turned on, the photosensitive chip assembly 65 collects images, and the position of the sample can be determined by software, thereby determining the sample area 71, and the area without the sample is the non-sample area 72. After the sample area 71 is determined, the backlight assembly 66 can be controlled to light up partially, that is, only the sample area 71 with the sample is lit, and the non-sample area is not lit. The photosensitive chip assembly 65 obtains the image again, and then images the sample, so that the sample can be better detected in this way.

[0067] The present invention belongs to a technology for detecting chemiluminescence in photosensitive imaging. The back-illuminated structured light projection imaging technology of the present invention can provide background light only for the target area (sample area), so that the light difference between the sample area and the non-sample area is obvious, and the image signal-to-noise ratio is increased. This is because, if the background light is only provided for the sample area, the periphery of the sample area is darker, and the image formed by the sample area is clearer. On the contrary, if the background light is provided for the entire area of ​​the photosensitive chip component, the image is unclear, just like overexposure when taking a photo. In addition, the back-illuminated structured light projection imaging technology of the present invention uses different color structured lights and light intensity detection chips to form a color image through a software algorithm, and the photosensitive chip component is large and can carry multiple samples, supporting simultaneous detection of multiple samples.

[0068] like Figure 10-16 , shows the structure of the automatic warehouse entry and exit device 11 according to an embodiment of the present invention, including the open state and the closed state. The automatic warehouse entry and exit device 11 mainly includes a frame 21, a warehouse door 22, a fixed base 23, a driving assembly 24, a transmission element 25, a power transmission assembly 26, two supporting guide elements 29, two slider assemblies 30 and a support plate 31. The fixed base 23 is a bearing element and is in the shape of a flat plate. It can cooperate with the cover 12 to form a closed structure. The frame 21 is configured to be able to move along the fixed base 23, the warehouse door 22 is arranged on the frame 21, and the driving assembly 24 is fixedly arranged on the fixed base 23. Here, the driving assembly 24 includes a motor and a reducer. The transmission element 25 is arranged between the driving assembly 24 and the frame 21, and is used to transmit the actuating force of the driving assembly 24 to the frame 21.

[0069] The two support guide elements 29 can be set as two slide rails, extending along the moving direction of the frame 21, and the two support guide elements 29 are respectively located at the lower part of both sides of the frame 21; the two slider assemblies 30 are indirectly connected to the frame 21, and the two slider assemblies 30 are respectively matched with the two support guide elements 29, so that the frame 21 can slide along the support guide elements 29; the support plate 31 is located in the frame 21, the frame 21 is fixed on the support plate 31, and one end of the support plate 31 extends out of the frame 21; the power transmission assembly 26 is fixed on the one end of the support plate 31, and the power transmission assembly 26 is connected to the transmission element 25. The transmission element 25 is centrally located between the two support guide elements 29, and the drive assembly 24 is located at one end of the transmission element 25.

[0070] In one embodiment, the transmission element 25 is configured as a transmission belt, which is sleeved on a driving wheel 27 and a driven wheel 28, the driving wheel 27 is in transmission connection with the driving assembly 24, and the driven wheel 28 is disposed on a side of the fixed base 23 away from the driving wheel 27. The power transmission assembly 26 includes an L-shaped plate and a clamp, one arm of the L-shaped plate is fixed to the one end of the support plate 31, and the other arm of the L-shaped plate is connected to the clamp to clamp the transmission belt.

[0071] It should be noted that the transmission elements of the present invention are not limited to synchronous belts, but can also be steel wires, ropes, lead screws, ball screws, gear racks, etc. The driving components can be pumps other than motors, and the supporting and guiding elements can also be linear guides, lead screws, guide shafts, etc.

[0072] Furthermore, the automatic storage device 11 further includes a circuit board 34, two sensors 32 and a sensing element 33. The circuit board 34 is in the shape of a longitudinal strip, located beside the frame 21 and arranged on the fixed base 23; the two sensors 32 are respectively arranged on the two ends of the circuit board 34; the sensing element 33 is arranged on the one end of the support plate 31, and the sensing element 33 is L-shaped and fixed on the support plate 31 by screws. The vertical lower end of the sensing element 33 can extend into the sensor 32, and the sensing of the sensing element 33 by the sensor 32 is used to determine whether the automatic storage device 11 is open or closed, and whether it is fully open or fully closed. The sensing device composed of the sensor 32 and the sensing element 33 of the present invention can be in the following forms: photoelectric sensor, micro switch, Hall sensor or other forms.

[0073] When in use, when the user sends a warehouse opening signal, the driving component 24 drives the transmission element 25 to move, and the transmission element 25 transmits power to the support plate 31 through the power transmission component 26, thereby causing the frame 21 to move outward. When the sensor 32 detects that the warehouse exit is completed, the driving component 24 stops moving and self-locks. When the user sends a warehouse entry signal, the transmission element 25 moves in the opposite direction, and the transmission element 25 transmits power to the support plate 31 through the power transmission component 26, thereby causing the frame 21 to move inward. When the sensor 32 detects that the warehouse entry is completed, the driving component 24 stops moving and self-locks.

[0074] In order to ensure that the door can always be tightly closed without being affected by assembly errors or displacement during use, the present invention improves and optimizes the connection structure of the door 22. Figure 17-21 Specifically, a door insertion strip 48 is provided on the back side of the door 22, and an insertion hole 47 is provided on the frame 21. The door insertion strip 48 extends into the insertion hole 47 and is directly or indirectly connected to the support plate 31. During assembly, the door 22 can be installed last after the components in the frame 21 are assembled.

[0075] Advantageously, the door 22 is configured to have a movement margin in multiple directions relative to the support plate 31; and the outer periphery of the door 22 forms a conical surface, so that the cross-sectional area of ​​the door 22 perpendicular to the direction from the door 22 to the frame 21 gradually decreases, see Fig.19 , the outer peripheral edge of the door 22 is an inclined surface, and correspondingly, the edge where the cover 12 and the door 22 are adapted is also an inclined surface. It is easy to know that when the door 22 has a slight assembly error, or as the position of the components changes during use (because the door is always extending and retracting, such displacement is easy to occur), the door 22 may not be aligned with the cover 12, that is, it cannot be perfectly closed. However, according to the design concept of the present invention, the edge of the door is designed to be a conical surface, and the door 22 can have a certain movement margin in the front-back, up-down, left-right directions, then such assembly errors or displacements can be compensated, so that the door can always be closed tightly.

[0076] To this end, the following design is added to the automatic entry and exit device 11, including a fixing column 41, a spring 42, a spring fixing bolt 43, a guide limit screw 44, a bin door fixing block 45, and a locking element 46. The fixing column 41 is fixedly arranged on the support plate 31. A screw hole can be provided in advance on the lower surface of the support plate 31. The fixing column 41 can be in the form of a bolt and screwed into the screw hole. The fixing column 41 serves as a supporting point for the spring 42.

[0077] The door fixing block 45 is Y-shaped. Fig.21As shown, it includes a first wall 52 located on one side, a second wall 53 and a third wall 54 located on the other side, a hollow space is formed between the second wall 53 and the third wall 54, and the door insertion strip 48 is inserted into the hollow space. The door fixing block 45 is provided with a spring fixing bolt screw hole 49, a guide limit screw through hole 50, and a locking element screw hole 51 along the frame 21, and the spring fixing bolt screw hole 49, the guide limit screw through hole 50, and the locking element screw hole 51 are arranged in sequence along the moving direction of the frame 21. The spring fixing bolt screw hole 49 and the guide limit screw through hole 50 are arranged on the first wall, and the locking element screw hole 51 is arranged on the second wall.

[0078] The spring fixing bolt 43 is fixed to the door fixing block 45 through the spring fixing bolt screw hole 49, and the spring fixing bolt 43 serves as another supporting point of the spring 42, so that the two ends of the spring 42 are respectively fixed to the fixing column 41 and the spring fixing bolt 43. In addition, the door fixing block 45 has a guide limit screw through hole 50, and the door fixing block 45 is configured to be attached to the support plate 31 through the guide limit screw 44. The lower surface of the support plate 31 is pre-set with a screw hole, and the guide limit screw 44 passes through the guide limit screw through hole 50 and is screwed on the screw hole. The inner diameter of the guide limit screw through hole 50 is larger than the outer diameter of the guide limit screw 44, and the degree of connection between the guide limit screw 44 and the support plate 31 allows the warehouse door fixing block 45 to have a movement margin in multiple directions relative to the support plate 31. That is to say, the reserved height of the guide limit screw 44 is greater than the height of the warehouse door fixing block, so that the warehouse door fixing block 45 can move slightly in the front and back, up and down, left and right directions. The warehouse door insertion strip 48 is connected to the warehouse door fixing block 45, so it will drive the warehouse door 22 to achieve the above-mentioned movement.

[0079] The locking element 46 is used to lock the door insertion strip 48 with the door fixing block 45; the locking element 46 includes a stud, an elastic element arranged in the stud, and a ball located at the end of the elastic element and extending from the stud; a locking element screw hole 51 is provided on the second wall 53 of the door fixing block 45, and the locking element 46 is screwed on the locking element screw hole 51, and the ball extends into the hollow space; the door insertion strip 48 is provided with a groove adapted to the ball.

[0080] As mentioned above, the door fixing block is fixed by the guide limit screw and the spring. The spring is in a pre-stretched state. The door fixing block can be appropriately moved and rotated along the guide limit screw, so that the door is in a floating state under the action of the spring force after assembly. During assembly, the door is fixed after being pushed into the door fixing block, and the assembly is simple. The edges of the door and the cover are treated with conical surfaces, which can automatically guide the door. When there is a small error in the assembly of parts, the floating function of the door fixing block and the conical surface of the door will ensure that the door is closed according to the conical surface guidance, which can effectively prevent light, air and foreign matter from entering.

[0081] It can be seen from the above technical scheme that the actuating mechanism of the automatic warehouse entry and exit device of the present invention is composed of a driving assembly, a transmission element, two supporting guide elements, two slider assemblies, a support plate, a power transmission assembly, a driven wheel, etc., and no exposed gear rack structure is used. The automatic warehouse entry and exit device adopts a tail-centered force-pulling structure. The transmission part and the dragging part are not exposed, and the appearance is beautiful. The force-generating position conforms to the symmetrical structure, which reduces the jamming and distortion during the process of entering and exiting the warehouse. In addition, the warehouse door is floatingly assembled, and it has a movement margin in multiple directions relative to the fixed support plate. Therefore, it can self-adjust to adapt to assembly errors or displacements during use, so that the warehouse door can always be closed tightly, ensuring a light-free and dust-free environment inside, and improving imaging quality.

[0082] The present invention also provides a photosensitive imaging method using the above-mentioned photosensitive imaging device, and the photosensitive imaging method comprises:

[0083] Step S1: Open the door 22 of the automatic loading and unloading device 11 of the photosensitive imaging device 10 to expose the photosensitive chip assembly 65;

[0084] Step S2: placing the sample 70 on the photosensitive chip assembly 65, and then closing the chamber door 22; the sample can be placed directly on the photosensitive chip assembly or indirectly on it;

[0085] Step S3: Light up the entire backlight assembly 66 to illuminate the photosensitive chip assembly 65 and the sample 70 on the photosensitive chip assembly 65;

[0086] Step S4: the photosensitive chip assembly 65 collects images and identifies the location of the sample 70, determines the sample area 71 and the non-sample area 72; and also includes identifying the shape of the sample area;

[0087] Step S5: sequentially lighting up the local backlight assembly 66 with different colors of light, so that only the sample area 71 is provided with backlight, and the non-sample area 72 is not provided with backlight; increasing the signal-to-noise ratio between the sample area and the non-sample area, and achieving higher sensitivity;

[0088] Step S6: The signal value of the sample 70 with different color backlights is obtained through the photosensitive chip component 65, and a color image of the sample 70 is synthesized in combination with a software algorithm; specifically, a certain area on the sample has only one color instead of mixed colors, and the signal values ​​under different color backlights are different, and the differences obey a certain regularity. Through this regularity, it is possible to clearly know the color of the sample itself, so that a color image of the sample can be obtained.

[0089] According to a preferred embodiment of the present invention, the photosensitive imaging method further comprises:

[0090] The color of the backlight provided by the backlight assembly 66 is changed.

[0091] According to a preferred embodiment of the present invention, the photosensitive chip assembly 65 is a light intensity detection chip.

[0092] According to a preferred embodiment of the present invention, the photosensitive imaging method forms a color image by using different color backlights and light intensity detection chips through a software algorithm.

[0093] According to a preferred embodiment of the present invention, multiple samples 70 are tested simultaneously. Since backlight is only provided to the target area, multiple samples can be tested simultaneously, effectively improving the testing efficiency.

[0094] The present invention belongs to a technology for detecting chemiluminescence in photosensitive imaging. The photosensitive imaging method of the present invention can provide background light only for the target area (sample area), so that the light difference between the sample area and the non-sample area is obvious, and the image signal-to-noise ratio is increased. This is because, if the background light is only provided for the sample area, then the periphery of the sample area is darker, and the image formed by the sample area is clearer. On the contrary, if the background light is provided for the entire area of ​​the photosensitive chip assembly, then the image is unclear, just like overexposure when taking a photo. In addition, the photosensitive imaging method of the present invention uses different color structured lights and light intensity detection chips to form a color image through a software algorithm, and the photosensitive chip assembly is large, can carry multiple samples, and supports simultaneous detection of multiple samples.

[0095] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes may be made to these embodiments without departing from the principles and spirit of the present invention. The scope of application of the present invention is defined by the appended claims and their equivalents.

[0096] List of reference numerals:

[0097] 10 Photosensitive imager

[0098] 11Automatic in and out warehouse device

[0099] 12 Shell

[0100] 13 Indicator lights

[0101] 14 Control switch

[0102] 21 Frame

[0103] 22 Warehouse door

[0104] 23Fixed base

[0105] 24 drive components

[0106] 25 Transmission elements

[0107] 26 Power transmission components

[0108] 27 driving wheel

[0109] 28 driven wheel

[0110] 29 Support guide element

[0111] 30 Slider assembly

[0112] 31 Support plate

[0113] 32 sensors

[0114] 33 Sensing element

[0115] 34 Circuit Board

[0116] 41Fixed column

[0117] 42 Spring

[0118] 43 Spring fixing screw

[0119] 44 guide limit screw

[0120] 45 Warehouse door fixing block

[0121] 46 locking elements

[0122] 47 Insertion hole

[0123] 48 Door Insert Strip

[0124] 49 Spring fixing bolt screw hole

[0125] 50 guide limit screw through hole

[0126] 51 Locking element screw hole

[0127] 52 First Wall

[0128] 53 Second Wall

[0129] 54 The Third Wall

[0130] 61U-Shaped Hood

[0131] 62 System Control Components

[0132] 63 Central Control Components

[0133] 64 Support fixings

[0134] 65 photosensitive chip components

[0135] 66 Backlight components

[0136] 67 wire

[0137] 68 Power switch

[0138] 69 transmission port

[0139] 70 samples

[0140] 71 Sample Area

[0141] 72 Non-sample area

[0142] 73 photosensitive chip control component.

Claims

1. A photosensitive imaging method, characterized in that: The photosensitive imaging method comprises: Step S1: opening the door (22) of the automatic entry and exit device (11) of the photosensitive imaging device (10) to expose the photosensitive chip assembly (65); Step S2: placing the sample (70) on the photosensitive chip assembly (65), and then closing the chamber door (22); Step S3: lighting up the entire backlight assembly (66) to illuminate the photosensitive chip assembly (65) and the sample (70) on the photosensitive chip assembly (65); Step S4: the photosensitive chip assembly (65) collects images and identifies the location of the sample (70), and determines the sample area (71) and the non-sample area (72); Step S5: sequentially lighting up the local backlight assembly (66) with lights of different colors, so that only the sample area (71) is provided with backlight, and the non-sample area (72) is not provided with backlight; Step S6: Acquire the signal values ​​of the samples (70) with different color backlights through the photosensitive chip assembly (65), and synthesize the color images of the samples (70) in combination with the software algorithm.

2. The photosensitive imaging method according to claim 1, characterized in that: The photosensitive imaging method also includes: Changing the color of the backlight provided by the backlight assembly (66).

3. The photosensitive imaging method according to claim 2, characterized in that: The photosensitive chip assembly (65) is a light intensity detection chip.

4. The photosensitive imaging method according to claim 3, characterized in that: The photosensitive imaging method forms a color image by using different color backlights and light intensity detection chips through software algorithms.

5. The photosensitive imaging method according to claim 4, characterized in that: Multiple samples (70) are tested simultaneously.

6. The photosensitive imaging method according to claim 1, characterized in that: The photosensitive imaging method uses a photosensitive imager (10), and the photosensitive imager (10) comprises: The automatic in-and-out storage device (11); The photosensitive chip assembly (65) is arranged on the automatic storage device (11) and is configured to be able to move with the automatic storage device (11) between a first position located inside the photosensitive imager (10) and a second position extending outside the photosensitive imager (10); and The backlight assembly (66) is arranged inside the photosensitive imager (10) and faces the photosensitive chip assembly (65) in the first position.

7. The photosensitive imaging method according to claim 6, characterized in that: The photosensitive imager (10) further comprises: The system control component (62) is fixed on the fixed base (23) and the backlight component (66), and is configured to convert the external power supply into the power required by the photosensitive imager (10), and control the in-and-out operation of the automatic in-and-out device (11) and the operation of the backlight component (66), and provide an input and output interface and a wireless connection interface for transmitting data; A central control component (63), fixed on the system control component (62), is used to provide human-computer interaction services for users; and The photosensitive chip control component (73) is configured to adjust and control the photosensitive chip component (65) to obtain sample luminescence data.

8. The photosensitive imaging method according to claim 7, characterized in that: At least one layer of coating is provided on the surface of the photosensitive chip component (65) facing the backlight component (66), and the coating is used to contact with the sample (70) and carry the sample (70).

9. The photosensitive imaging method according to claim 7, characterized in that: An indicator light (13) and a control switch (14) are provided on the cover (12) of the photosensitive imager (10); the indicator light (13) is used to indicate the working state of the photosensitive imager (10); and the control switch (14) is used to control the opening and closing of the automatic in-and-out bin device (11).

10. The photosensitive imaging method according to claim 7, characterized in that: The automatic in-and-out warehouse device (11) comprises: The fixed base (23); a frame (21), the frame (21) being configured to be movable along a fixed base (23); A door (22) is arranged on the frame (21); A drive assembly (24), wherein the drive assembly (24) is fixedly disposed on the fixed base (23); and The transmission element (25) is arranged between the driving assembly (24) and the frame (21) and is used to transmit the actuating force of the driving assembly (24) to the frame (21).

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