Preparation method of novel disease-resistant digestion-aiding feed additive

Abstract

The invention relates to the technical field of feed additives, and discloses a preparation method of a novel disease-resistant digestion-aiding feed additive. The radix tinosporae, andrographis paniculata saponin and polysaccharide have good antibacterial and disease-resistant effects, and can effectively prevent livestock from being infected by germs. The inorganic composite product is prepared from inorganic matters and has the effects of promoting digestion, sterilizing and expelling parasites. The powdery fermentation product is prepared by mixing and fermenting myristica fragrans, sweet wormwood, hawthorn and sea-buckthorn, has the effects of promoting digestion, resisting diseases, increasing appetite and the like, and promotes the growth of poultry. The membrane material is a good antibacterial and corrosion-resistant material, and can effectively prevent the feed additive from being rotten and prolong the shelf life. The feed additive disclosed by the invention has good disease-resistant and digestion-aiding effects and can be widely applied to production of feeds and medicines, and the process production is green, safe and efficient.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, in particular to a method for preparing a novel disease-resistant and digestion-aiding feed additive. Background Art

[0002] The development of animal husbandry in my country has gradually moved towards industrialization, and the requirements for feed production and quality are getting higher and higher. Traditional feed cannot meet the requirements of industrial animal husbandry. With the industrialization of animal husbandry, the pursuit of production requirements is getting higher and higher, which will lead to problems such as indigestion caused by overfeeding and diseases caused by intensive breeding. Therefore, while feeding feed, adding an anti-disease and digestive additive to the feed can not only meet the requirements of efficient breeding, but also ensure the health of the breeding process. Since most additives are now chemically synthesized drugs, there will be drug residues, which have a certain impact on food health and safety. The use of natural materials, pollution-free and residue-free characteristics have attracted widespread attention. Summary of the invention

[0003] 1. Technical issues to be resolved In view of the deficiencies of the prior art, the present invention provides a method for preparing a novel disease-resistant and digestion-aiding feed additive.

[0004] (II) Technical solution A method for preparing a novel disease-resistant and digestion-aiding feed additive comprises the following steps: S1. Slice the golden fruit olive, dry it in the sun, crush it, and pass it through a 40-mesh sieve. Dry it in the sun, crush it, and pass it through a 40-mesh sieve. Mix them and add them into 10-20 times the weight of distilled water. Stir and extract them in a 40-60℃ water bath shaker for 10-30 min. Then, treat them with microwaves. The treatment conditions are 200-400 W, 5-10 min, and 70-80℃ water bath for 1-2 h. Filter them with suction. Add the residue into distilled water. Repeat the above operation. Combine the two filtrates, pass them through a 0.2-0.5 μm microfiltration membrane, and pass the permeate through an ultrafiltration membrane with a molecular weight cutoff of 5000-10000 to obtain the permeate and the concentrate. S2. The permeate was concentrated to one tenth of the original volume at 0.05-0.1 MPa and 70-80°C, freeze-dried, and chromatographed on an NKA macroporous adsorption resin column, eluted with 80% ethanol, the eluate was collected, dehydrated with anhydrous sodium sulfate, and then crystallized with ether to obtain saponin; the concentrate was vacuum concentrated at 0.05-0.1 MPa and 70-80°C, and the protein was removed by Sevage method. After dialysis for 12-24 hours, the polysaccharide was vacuum dried; S3, fry the salt until it turns yellow, add quicklime and continue frying for 10-30 min, and when the temperature drops to 30-40°C, add sulfur powder and nano-silicon dioxide to mix and obtain an inorganic composite product; S4. Press nutmeg, artemisia annua, hawthorn and sea buckthorn until they are crushed, mix and stir, place in a fermentation tank, adjust the pH to neutral, inoculate yeast and lactobacillus, and perform aerobic fermentation. The fermentation conditions are a rotation speed of 200-300 r / min and a ventilation volume of 0.05-0.1 m 3 / min, the temperature is 35-38°C, and after the fermentation is completed, the fermented product is dried and crushed to obtain a powdered fermentation product; S5, dissolve chitosan in 0.1 mol / L acetic acid solution, add glycidyl trimethylammonium chloride, stir evenly, react at 70-90°C for 4-8 h, after the reaction, cool to room temperature, adjust pH to 4-5, add cysteine, place in a constant temperature oscillator at 40-50°C for 3-6 h, place overnight, wash with water until neutral, and obtain a membrane material; S6. Mix saponin, polysaccharide, inorganic composite product and powdered fermentation product, stir evenly, place in the bellows of spray dryer, turn on the blower, spray-coat the film material in the form of spray, and dry at the same time to obtain disease-resistant and digestive-aiding feed additive.

[0005] Preferably, in step S1, the mass ratio of Herba Andrographis paniculatae to Herba Andrographis paniculatae is 1:0.8-1.2.

[0006] Preferably, in step S3, the mass ratio of salt, quicklime, sulfur powder and nano-silicon dioxide is 1:0.8~1.2:0.8~1.2:0.3~0.6.

[0007] Preferably, in step S4, the mass ratio of nutmeg, artemisia annua, hawthorn, seabuckthorn, yeast and lactobacillus is 1:1-1.2:2-4:0.8-1.6:0.02-0.05:0.08-0.15.

[0008] Preferably, in step S5, the mass ratio of chitosan, glycidyl trimethylammonium chloride and cysteine ​​is 1:1.5~3:1~1.5.

[0009] Preferably, in step S6, the mass ratio of the powdered fermentation product, the inorganic composite product, the saponin, the polysaccharide, and the membrane material is 1:0.05~0.1:0.01~0.02:0.01~0.02:0.8~1.2.

[0010] Preferably, the disease-resistant and digestion-aiding feed additive is used in the preparation of a feed additive.

[0011] Preferably, the disease-resistant and digestive-aiding feed additive is used in the preparation of disease-resistant and digestive-aiding drugs.

[0012] 3. Beneficial technical effects The disease-resistant and digestive-aiding feed additive produced by the present invention is made of golden olive, andrographol saponin and polysaccharide, inorganic composite product, and powdered fermentation product coated with film material. Golden olive, andrographol saponin and polysaccharide have good antibacterial and disease-resistant effects, and can effectively prevent livestock and poultry from being infected by pathogens. The inorganic composite product is made of inorganic substances and has the effects of aiding digestion, killing bacteria and expelling insects. The powdered fermentation product is made of nutmeg, wormwood, hawthorn, and sea buckthorn mixed fermentation, which can promote digestion, resist disease, increase appetite and other effects to promote the growth of livestock and poultry. The film material is a good antibacterial and corrosion-resistant material, which can effectively prevent the decay of feed additives and extend the shelf life.

[0013] The feed additive of the invention has good disease resistance and digestion-aiding effects, can be widely used in the production of feed and medicine, and the production process is green, safe and efficient. DETAILED DESCRIPTION

[0014] Example 1: S1, 10 kg of golden olive slices, dried, crushed, and passed through a 40-mesh sieve, 8 kg of Andrographis paniculata dried, crushed, and passed through a 40-mesh sieve, mixed and added into 10 times the weight of distilled water, stirred and extracted in a 40°C water bath shaker for 10 min, assisted by microwave treatment, the treatment conditions are 200 W, 5 min, 70°C water bath for 1 h, filtered, the residue is added into distilled water, the above operation is repeated, the two filtrates are combined, passed through a 0.2 μm microfiltration membrane, the permeate is passed through an ultrafiltration membrane with a molecular weight cutoff of 5000, to obtain a permeate and a concentrate.

[0015] S2. Concentrate the permeate to one tenth of its original volume at 0.05 MPa and 70°C, freeze-dry, and apply to NKA macroporous adsorption resin column for chromatography. Elute with 80% ethanol, collect the eluate, dehydrate with anhydrous sodium sulfate, and crystallize with ether to obtain saponin; concentrate at 0.05 MPa and 70°C in a vacuum concentration, remove protein with the Sevage method, dialyze for 12 h, and then vacuum dry to obtain polysaccharides.

[0016] S3. Fry 10 g of salt until it turns yellow, add 8 g of quicklime and continue frying for 10 min. When the temperature drops to 30°C, add 8 g of sulfur powder and 3 g of nano-silicon dioxide and mix to obtain an inorganic composite product.

[0017] S4. Press 100 g of nutmeg, 100 g of Artemisia annua, 200 g of hawthorn, and 80 g of sea buckthorn until they are crushed, mix and stir, place in a fermentation tank, adjust the pH to neutral, inoculate 2 g of yeast and 8 g of lactobacillus, and perform aerobic fermentation. The fermentation conditions are a rotation speed of 200 r / min and a ventilation volume of 0.05 m 3 / min, the temperature is 35°C. After the fermentation is completed, the fermented material is dried and crushed to obtain a powdered fermentation product.

[0018] S5. Dissolve 100 g of chitosan in 3 L of 0.1 mol / L acetic acid solution, add 150 g of glycidyl trimethylammonium chloride, stir evenly, react at 70 °C for 4 h, after the reaction is completed, cool the temperature to room temperature, adjust the pH to 4, add 100 g of cysteine, place in a constant temperature oscillator at 40 °C for 3 h, leave overnight, wash with water until neutral, and obtain a membrane material.

[0019] S6. Mix 1 g of saponin, 1 g of polysaccharide, 5 g of inorganic composite product, and 100 g of powdered fermentation product, stir evenly, place in the bellows of a spray dryer, turn on the blower, spray-coat 80 g of the film material in the form of a spray, and dry at the same time to obtain a disease-resistant and digestive-aiding feed additive.

[0020] Example 2: S1, 10 kg of golden olive slices, dried, crushed, and passed through a 40-mesh sieve, 12 kg of Andrographis paniculata dried, crushed, and passed through a 40-mesh sieve, mixed and added into 20 times the weight of distilled water, stirred and extracted in a 60°C water bath shaker for 30 min, assisted by microwave treatment, the treatment conditions are 400 W, 10 min, 80°C water bath for 2 h, filtered, the residue is added into distilled water, the above operation is repeated, the two filtrates are combined, passed through a 0.5 μm microfiltration membrane, the permeate is passed through an ultrafiltration membrane with a molecular weight cutoff of 10,000, to obtain a permeate and a concentrate.

[0021] S2. Concentrate the permeate to one tenth of its original volume at 0.1 MPa and 80°C, freeze-dry, and apply to NKA macroporous adsorption resin column for chromatography. Elute with 80% ethanol, collect the eluate, dehydrate with anhydrous sodium sulfate, and crystallize with ether to obtain saponin; concentrate at 0.1 MPa and 80°C in a vacuum concentration, remove protein with the Sevage method, dialyze for 24 hours, and then vacuum dry the polysaccharide.

[0022] S3. Fry 10 g of salt until it turns yellow, add 12 g of quicklime and continue frying for 30 min. When the temperature drops to 40°C, add 12 g of sulfur powder and 6 g of nano-silicon dioxide and mix to obtain an inorganic composite product.

[0023] S4. Press 100 g of nutmeg, 120 g of Artemisia annua, 400 g of hawthorn, and 160 g of sea buckthorn until they are crushed, mix and stir, place in a fermentation tank, adjust the pH to neutral, inoculate 5 g of yeast and 15 g of lactobacillus, and perform aerobic fermentation. The fermentation conditions are a rotation speed of 300 r / min and a ventilation volume of 0.1 m 3 / min, the temperature is 38°C. After the fermentation is completed, the fermented material is dried and crushed to obtain a powdered fermentation product.

[0024] S5. Dissolve 100 g of chitosan in 3 L of 0.1 mol / L acetic acid solution, add 300 g of glycidyl trimethylammonium chloride, stir evenly, react at 90 °C for 8 h, after the reaction is completed, cool the temperature to room temperature, adjust the pH to 5, add 150 g of cysteine, place in a constant temperature oscillator at 50 °C for 6 h, leave overnight, wash with water until neutral, and obtain a membrane material.

[0025] S6. Mix 2 g of saponin, 2 g of polysaccharide, 10 g of inorganic composite product, and 100 g of powdered fermentation product, stir evenly, place in the bellows of a spray dryer, turn on the blower, spray-coat 120 g of the film material in the form of a spray, and dry it at the same time to obtain a disease-resistant and digestive-aiding feed additive.

[0026] Example 3: S1, 10 kg of golden olive slices, dried, crushed, and passed through a 40-mesh sieve, 10 kg of Andrographis paniculata dried, crushed, and passed through a 40-mesh sieve, mixed and added into 15 times the weight of distilled water, stirred and extracted in a 50°C water bath shaker for 20 min, assisted by microwave treatment, the treatment conditions are 300 W, 5 min, 80°C water bath for 1.5 h, filtered, the residue is added into distilled water, the above operation is repeated, the two filtrates are combined, passed through a 0.3 μm microfiltration membrane, the permeate is passed through an ultrafiltration membrane with a molecular weight cutoff of 6000, to obtain a permeate and a concentrate.

[0027] S2. Concentrate the permeate to one tenth of its original volume at 0.06 MPa and 75°C, freeze-dry, and apply to NKA macroporous adsorption resin column for chromatography. Elute with 80% ethanol, collect the eluate, dehydrate with anhydrous sodium sulfate, and crystallize with ether to obtain saponin; concentrate at 0.08 MPa and 80°C in a vacuum concentration, remove protein with the Sevage method, dialyze for 15 hours, and then vacuum dry to obtain polysaccharides.

[0028] S3. Fry 10 g of salt until it turns yellow, add 9 g of quicklime and continue frying for 15 min. When the temperature drops to 35°C, add 10 g of sulfur powder and 5 g of nano-silicon dioxide and mix to obtain an inorganic composite product.

[0029] S4. Press 100 g of nutmeg, 110 g of Artemisia annua, 250 g of hawthorn, and 90 g of sea buckthorn until they are crushed, mix and stir, place in a fermentation tank, adjust the pH to neutral, inoculate 3 g of yeast and 9 g of lactobacillus, and perform aerobic fermentation. The fermentation conditions are a rotation speed of 250 r / min and a ventilation volume of 0.08 m 3 / min, the temperature is 37°C. After the fermentation is completed, the fermented material is dried and crushed to obtain a powdered fermentation product.

[0030] S5. Dissolve 100 g of chitosan in 3 L of 0.1 mol / L acetic acid solution, add 200 g of glycidyl trimethylammonium chloride, stir evenly, react at 80°C for 4 h, after the reaction is completed, cool the temperature to room temperature, adjust the pH to 4, add 120 g of cysteine, place in a constant temperature oscillator at 45°C for 5 h, leave overnight, wash with water until neutral, and obtain a membrane material.

[0031] S6. Mix 1.2 g of saponin, 1.5 g of polysaccharide, 6 g of inorganic composite product, and 100 g of powdered fermentation product, stir evenly, place in the bellows of a spray dryer, turn on the blower, spray-coat 90 g of the film material in the form of a spray, and dry it at the same time to obtain a disease-resistant and digestive-aiding feed additive.

[0032] Example 4: S1, 10 kg of golden olive slices, dried, crushed, and passed through a 40-mesh sieve, 9 kg of Andrographis paniculata dried, crushed, and passed through a 40-mesh sieve, mixed and added into 20 times the weight of distilled water, stirred and extracted in a 60°C water bath shaker for 20 min, assisted by microwave treatment, the treatment conditions are 400 W, 10 min, 70°C water bath for 1.2 h, filtered, the residue is added into distilled water, the above operation is repeated, the two filtrates are combined, passed through a 0.4 μm microfiltration membrane, the permeate is passed through an ultrafiltration membrane with a molecular weight cutoff of 10,000, to obtain a permeate and a concentrated solution.

[0033] S2. Concentrate the permeate to one tenth of its original volume at 0.09 MPa and 80°C, freeze-dry, and apply to NKA macroporous adsorption resin column for chromatography. Elute with 80% ethanol, collect the eluate, dehydrate with anhydrous sodium sulfate, and crystallize with ether to obtain saponin; concentrate at 0.05 MPa and 80°C in a vacuum concentration, remove protein with the Sevage method, dialyze for 20 hours, and then vacuum dry to obtain polysaccharides.

[0034] S3. Fry 10 g of salt until it turns yellow, add 10 g of quicklime and continue frying for 30 min. When the temperature drops to 40°C, add 11 g of sulfur powder and 5 g of nano-silicon dioxide and mix to obtain an inorganic composite product.

[0035] S4. Press 100 g of nutmeg, 120 g of Artemisia annua, 300 g of hawthorn, and 120 g of sea buckthorn until they are crushed, mix and stir, place in a fermentation tank, adjust the pH to neutral, inoculate 4 g of yeast and 12 g of lactobacillus, and perform aerobic fermentation. The fermentation conditions are a rotation speed of 260 r / min and a ventilation volume of 0.1 m 3 / min, the temperature is 36°C. After the fermentation is completed, the fermented material is dried and crushed to obtain a powdered fermentation product.

[0036] S5. Dissolve 100 g of chitosan in 3 L of 0.1 mol / L acetic acid solution, add 250 g of glycidyl trimethylammonium chloride, stir evenly, react at 80 °C for 6 h, after the reaction is completed, cool the temperature to room temperature, adjust the pH to 5, add 140 g of cysteine, place in a constant temperature oscillator at 50 °C for 6 h, leave overnight, wash with water until neutral, and obtain a membrane material.

[0037] S6. Mix 1.5 g of saponin, 2 g of polysaccharide, 10 g of inorganic composite product, and 100 g of powdered fermentation product, stir evenly, place in the bellows of a spray dryer, turn on the blower, spray-coat 120 g of the film material in the form of a spray, and dry it at the same time to obtain a disease-resistant and digestive-aiding feed additive.

[0038] Example 5: S1, 10 kg of golden olive slices, dried, crushed, and passed through a 40-mesh sieve, 12 kg of Andrographis paniculata dried, crushed, and passed through a 40-mesh sieve, mixed and added into 10 times the weight of distilled water, stirred and extracted in a 40°C water bath shaker for 10 min, assisted by microwave treatment, the treatment conditions are 200 W, 10 min, 70°C water bath for 2 h, filtered, the residue is added into distilled water, the above operation is repeated, the two filtrates are combined, passed through a 0.5 μm microfiltration membrane, the permeate is passed through an ultrafiltration membrane with a molecular weight cutoff of 5000, to obtain a permeate and a concentrated solution.

[0039] S2. Concentrate the permeate to one tenth of its original volume at 0.1 MPa and 70°C, freeze-dry, and apply to NKA macroporous adsorption resin column for chromatography. Elute with 80% ethanol, collect the eluate, dehydrate with anhydrous sodium sulfate, and crystallize with ether to obtain saponin; concentrate at 0.05 MPa and 70°C in a vacuum concentration, remove protein with the Sevage method, dialyze for 20 h, and then vacuum dry to obtain polysaccharides.

[0040] S3. Fry 10 g of salt until it turns yellow, add 10 g of quicklime and continue frying for 30 min. When the temperature drops to 30°C, add 9 g of sulfur powder and 5 g of nano-silicon dioxide and mix to obtain an inorganic composite product.

[0041] S4. Press 100 g of nutmeg, 120 g of Artemisia annua, 200 g of hawthorn, and 150 g of sea buckthorn until they are crushed, mix and stir, place in a fermentation tank, adjust the pH to neutral, inoculate 5 g of yeast and 15 g of lactobacillus, and perform aerobic fermentation. The fermentation conditions are a rotation speed of 300 r / min and a ventilation volume of 0.05 m 3 / min, the temperature is 35°C. After the fermentation is completed, the fermented material is dried and crushed to obtain a powdered fermentation product.

[0042] S5. Dissolve 100 g of chitosan in 3 L of 0.1 mol / L acetic acid solution, add 250 g of glycidyl trimethylammonium chloride, stir evenly, react at 70 °C for 5 h, after the reaction is completed, cool the temperature to room temperature, adjust the pH to 5, add 150 g of cysteine, place in a constant temperature oscillator at 50 °C for 6 h, leave overnight, wash with water until neutral, and obtain a membrane material.

[0043] S6. Mix 1.5 g of saponin, 1 g of polysaccharide, 5 g of inorganic composite product, and 100 g of powdered fermentation product, stir evenly, place in the bellows of a spray dryer, turn on the blower, spray-coat 80 g of the film material in the form of a spray, and dry it at the same time to obtain a disease-resistant and digestive-aiding feed additive.

[0044] Comparative Example 1 S1. Slice 10 kg of Oleander, dry it in the sun, crush it, and pass it through a 40-mesh sieve. Dried 8 kg of Andrographis paniculata in the sun, crush it, and pass it through a 40-mesh sieve. Mix them and add them into 10 times the weight of distilled water. Stir and extract them in a 40℃ water bath shaker for 10 min. Microwave treatment was used. The treatment conditions were 200 W for 5 min and 70℃ water bath for 1 h. Filter by suction. Add the residue into distilled water. Repeat the above operation. Combine the two filtrates, pass them through a 0.2 μm microfiltration membrane, and pass the permeate through an ultrafiltration membrane with a molecular weight cutoff of 5000 to obtain the permeate and concentrate.

[0045] S2. Concentrate the permeate to one tenth of its original volume at 0.05 MPa and 70°C, freeze-dry, and apply to NKA macroporous adsorption resin column for chromatography. Elute with 80% ethanol, collect the eluate, dehydrate with anhydrous sodium sulfate, and crystallize with ether to obtain saponin; concentrate at 0.05 MPa and 70°C in a vacuum concentration, remove protein with the Sevage method, dialyze for 12 h, and then vacuum dry to obtain polysaccharides.

[0046] S3. Pressurize 100 g of Artemisia annua to crush it, mix and stir, place in a fermentation tank, adjust the pH to neutral, inoculate 2 g of yeast and 8 g of Lactobacillus, and perform aerobic fermentation. The fermentation conditions are a speed of 200 r / min and a ventilation volume of 0.05 m 3 / min, the temperature is 35°C. After the fermentation is completed, the fermented material is dried and crushed to obtain a powdered fermentation product.

[0047] S4. Dissolve 100 g of chitosan in 3 L of 0.1 mol / L acetic acid solution, add 150 g of glycidyl trimethylammonium chloride, stir evenly, react at 70°C for 4 h, after the reaction is completed, cool the temperature to room temperature, adjust the pH to 4, add 100 g of cysteine, place in a constant temperature oscillator at 40°C for 3 h, leave overnight, wash with water until neutral, and obtain a membrane material.

[0048] S5. Mix 1 g of saponin, 1 g of polysaccharide and 100 g of powdered fermentation product, stir evenly, place in the bellows of a spray dryer, turn on the blower, spray-coat 80 g of the film material in the form of a spray, and dry at the same time to obtain a disease-resistant and digestive-aiding feed additive.

[0049] Comparative Example 2 S1. Fry 10 g salt until it turns yellow, add 8 g quicklime and continue frying for 10 min. When the temperature drops to 30°C, add 8 g sulfur powder and 3 g nano-silicon dioxide and mix to obtain an inorganic composite product.

[0050] S2. Press 100 g nutmeg, 200 g hawthorn, and 80 g sea buckthorn until they are crushed, mix and stir, place in a fermentation tank, adjust the pH to neutral, inoculate 2 g yeast and 8 g lactobacillus, and perform aerobic fermentation. The fermentation conditions are a rotation speed of 200 r / min and a ventilation volume of 0.05 m 3 / min, the temperature is 35°C. After the fermentation is completed, the fermented material is dried and crushed to obtain a powdered fermentation product.

[0051] S3. Dissolve 100 g of chitosan in 3 L of 0.1 mol / L acetic acid solution, add 150 g of glycidyl trimethylammonium chloride, stir evenly, react at 70°C for 4 h, after the reaction is completed, cool the temperature to room temperature, adjust the pH to 4, add 100 g of cysteine, place in a constant temperature oscillator at 40°C for 3 h, leave overnight, wash with water until neutral, and obtain a membrane material.

[0052] S4. Mix 5 g of the inorganic composite product and 100 g of the powdered fermentation product, stir evenly, place in the bellows of a spray dryer, turn on the blower, spray-coat 80 g of the film material in the form of a spray, and dry at the same time to obtain a disease-resistant and digestive-aiding feed additive.

[0053] 1. Digestive aid test 600 white-feathered broilers with low spirits, decreased feed intake, and residual feed particles and foam in feces were selected and diagnosed as indigestion. They were divided into 6 groups, each with 100 chickens, wherein the Example 1-5 groups were gavaged with 0.2 g / kg of the feed additives in Examples 1-5, and the Comparative Example 1 group was gavaged with 0.2 g / kg of the feed additives in Comparative Example 1, and the feeding was continued for 10 days to observe the digestion of the sick chickens.

[0054] Cure: Good spirits, normal feed intake, no feed residue particles or foam in feces.

[0055] Significant effect: The spirit is improved, the feed intake is close to normal, and there is basically no feed residual particles and foam in the feces.

[0056] Effective: Improved spirits, increased feed intake, and reduced feed particles and foam in feces.

[0057] Ineffective: No improvement in symptoms.

[0058] Total effective rate = (cured number + markedly effective number + effective number) / number of sick chickens tested × 100% Table 1

[0059] As shown in Table 1, the addition of the feed additives prepared in Examples 1 to 5 can effectively improve the indigestion symptoms of white-feathered broilers, with significant effects.

[0060] 2. Disease resistance test 600 5-day-old white-feathered broilers were selected and divided into groups 1 to 5 of embodiments and group 2 of comparative example, with 100 broilers in each group. Groups 1 to 5 of embodiments were gavaged with 0.2 g / kg of the feed additives in embodiments 1 to 5, and group 2 of comparative example was gavaged with 0.2 g / kg of the feed additives in comparative example 21. The gavage was performed 4 times, on the 5th day, the 10th day, the 15th day, and the 20th day, respectively, and the test period was 25 days, and the diarrhea of ​​the broilers was observed.

[0061] Table 2

[0062] It can be seen from Table 2 that the feed additives prepared in Examples 1 to 5 can effectively reduce the incidence of diarrhea with significant effects.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a novel disease-resistant and digestion-aiding feed additive, characterized in that: The following steps are involved: S1. Slice the golden fruit olive, dry it in the sun, crush it, and pass it through a 40-mesh sieve. Dry it in the sun, crush it, and pass it through a 40-mesh sieve. Mix them and add them into 10-20 times the weight of distilled water. Stir and extract them in a 40-60℃ water bath shaker for 10-30 min. Then, treat them with microwaves. The treatment conditions are 200-400 W, 5-10 min, and 70-80℃ water bath for 1-2 h. Filter them with suction. Add the residue into distilled water. Repeat the above operation. Combine the two filtrates, pass them through a 0.2-0.5 μm microfiltration membrane, and pass the permeate through an ultrafiltration membrane with a molecular weight cutoff of 5000-10000 to obtain the permeate and the concentrate. S2. The permeate was concentrated to one tenth of the original volume at 0.05-0.1 MPa and 70-80°C, freeze-dried, and chromatographed on an NKA macroporous adsorption resin column, eluted with 80% ethanol, the eluate was collected, dehydrated with anhydrous sodium sulfate, and then crystallized with ether to obtain saponin; the concentrate was vacuum concentrated at 0.05-0.1 MPa and 70-80°C, and the protein was removed by Sevage method. After dialysis for 12-24 hours, the polysaccharide was vacuum dried; S3, fry the salt until it turns yellow, add quicklime and continue frying for 10-30 min, and when the temperature drops to 30-40°C, add sulfur powder and nano-silicon dioxide to mix and obtain an inorganic composite product; S4. Press nutmeg, artemisia annua, hawthorn and sea buckthorn until they are crushed, mix and stir, place in a fermentation tank, adjust the pH to neutral, inoculate yeast and lactobacillus, and perform aerobic fermentation. The fermentation conditions are a rotation speed of 200-300 r / min and a ventilation volume of 0.05-0.1 m 3 / min, the temperature is 35-38°C, and after the fermentation is completed, the fermented product is dried and crushed to obtain a powdered fermentation product; S5, dissolve chitosan in 0.1 mol / L acetic acid solution, add glycidyl trimethylammonium chloride, stir evenly, react at 70-90°C for 4-8 h, after the reaction, cool to room temperature, adjust pH to 4-5, add cysteine, place in a constant temperature oscillator at 40-50°C for 3-6 h, place overnight, wash with water until neutral, and obtain a membrane material; S6. Mix saponin, polysaccharide, inorganic composite product and powdered fermentation product, stir evenly, place in the bellows of spray dryer, turn on the blower, spray-coat the film material in the form of spray, and dry at the same time to obtain disease-resistant and digestive-aiding feed additive.

2. The method for preparing a novel disease-resistant and digestion-aiding feed additive according to claim 1, characterized in that: In the step S1, the mass ratio of Olea europaea to Andrographis paniculata is 1:0.8-1.

2.

3. The method for preparing a novel disease-resistant and digestion-aiding feed additive according to claim 1, characterized in that: In step S3, the mass ratio of salt, quicklime, sulfur powder and nano silicon dioxide is 1:0.8~1.2:0.8~1.2:0.3~0.

6.

4. The method for preparing a novel disease-resistant and digestion-aiding feed additive according to claim 1, characterized in that: In the step S4, the mass ratio of nutmeg, artemisia annua, hawthorn, seabuckthorn, yeast and lactobacillus is 1:1-1.2:2-4:0.8-1.6:0.02-0.05:0.08-0.

15.

5. The method for preparing a novel disease-resistant and digestion-aiding feed additive according to claim 1, characterized in that: In the step S5, the mass ratio of chitosan, glycidyl trimethylammonium chloride and cysteine ​​is 1:1.5-3:1-1.

5.

6. The method for preparing a novel disease-resistant and digestion-aiding feed additive according to claim 1, characterized in that: In step S6, the mass ratio of the powdered fermentation product, the inorganic composite product, the saponin, the polysaccharide, and the membrane material is 1:0.05-0.1:0.01-0.02:0.01-0.02:0.8-1.

2.

7. Use of the disease-resistant and digestive-aiding feed additive prepared by the method for preparing a novel disease-resistant and digestive-aiding feed additive according to claim 1 in preparing feed additives.

8. Use of the disease-resistant and digestive-aiding feed additive prepared by the method for preparing a novel disease-resistant and digestive-aiding feed additive according to claim 1 in the preparation of disease-resistant and digestive-aiding drugs.

Images