Acne-removing and anti-inflammatory preparation for skin and preparation method of acne-removing and anti-inflammatory preparation

Abstract

The invention provides a skin acne-removing and anti-inflammatory preparation and a preparation method thereof, and belongs to the technical field of cosmetics. The preparation is prepared from the following raw materials in percentage by mass: 15 to 30 percent of acne-removing and anti-inflammatory functional components, 0.5 to 20 percent of emollient, 0.5 to 15 percent of emulsifier, 1 to 25 percent of filler, 1 to 15 percent of thickener, 0.05 to 0.3 percent of preservative, 0.01 to 0.5 percent of pH regulator, 0.005 to 0.05 percent of essence and the balance of water, the acne-removing and anti-inflammatory functional components are obtained by sequentially performing enzymolysis and fermentation on traditional Chinese medicine raw materials; the traditional Chinese medicine raw materials comprise radix paeoniae rubra, seaweed, honeysuckle, dandelion and rose; a composite enzymolysis agent is used for enzymolysis, and the composite enzymolysis agent comprises beta-galactosidase, coenzyme A, laccase and maltase; a composite leavening agent is used for fermentation, and the composite leavening agent comprises xylanolyticus, saccharopolyspora spinosa, bacillus licheniformis and candida tropicalis. The preparation prepared by the invention is reasonable in formula composition, not only has excellent acne-removing and anti-inflammatory effects, but also has certain stability.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and in particular relates to a skin acne-removing and anti-inflammatory preparation and a preparation method thereof. Background Art

[0002] Acne, also known as acne, also known as pimples, pimples, blackheads or acne, is a skin problem that people are more concerned about. It is prone to occur on the cheeks and forehead. The main causes of acne are excessive secretion of the cortex, abnormal keratinization of hair follicles, bacterial infection, hormonal changes, etc. When the sebaceous glands secrete a large amount of oil, the oil and dead skin cells block the pores, and the oil cannot be eliminated and accumulates in the hair follicles. At this time, bacteria will breed in the hair follicles, causing inflammation, resulting in redness and swelling, rupture of hair follicles, and damage to the skin barrier. The main factors for people who are prone to acne are excessive oily skin, eating habits, irregular lifestyle, high stress, etc., and some factors are difficult to avoid, so how to protect the skin from acne is a problem that many people are more concerned about.

[0003] Commonly used acne removal products include salicylic acid, phenoxyethanol, benzoyl peroxide, vitamin A acid and its derivatives, antibiotics, etc. However, the use of the above acne removal products has potential side effects, long-term use can easily lead to dependence, and cannot fundamentally solve the acne problem. Based on this, people tend to use acne removal products with natural ingredients as active ingredients. These products are milder than chemical synthetic ingredients, less irritating to the skin, suitable for sensitive skin, and also have anti-inflammatory, moisturizing and soothing effects, and antioxidant effects, etc., which can comprehensively regulate acne and skin conditions.

[0004] Although acne-removing products with natural ingredients have many advantages, they also have some disadvantages in actual use. For example, there are many natural ingredients, and the formula is difficult to match, making it difficult to achieve the best acne-removing and anti-inflammatory effects; natural ingredients are easily affected by the surrounding environment, and are unstable and deteriorate, losing their acne-removing and anti-inflammatory effects; and acne-removing products with natural ingredients are difficult to store. Summary of the invention

[0005] The purpose of the present invention is to provide a skin acne-removing and anti-inflammatory preparation and a preparation method thereof, so as to solve the problems of difficult compatibility, poor stability and difficult storage of the above-mentioned acne-removing and anti-inflammatory preparations with natural ingredients.

[0006] In order to achieve the above-mentioned object, the first aspect of the present invention provides a skin acne and anti-inflammatory preparation, which comprises the following raw materials in terms of mass percentage: 15-30% of acne and anti-inflammatory effective ingredients, 0.5-20% of emollients, 0.5-15% of emulsifiers, 1-25% of fillers, 1-15% of thickeners, 0.05-0.3% of preservatives, 0.01-0.5% of pH adjusters, 0.005-0.05% of flavors, and the balance of water;

[0007] The anti-acne and anti-inflammatory ingredients are obtained by sequential enzymatic hydrolysis and fermentation of Chinese medicinal materials;

[0008] The Chinese medicinal materials include red peony root, seaweed, honeysuckle, dandelion and rose in a mass ratio of (4-6):(1-3):(6-8):(10-12):1;

[0009] The enzymatic hydrolysis uses a composite enzymatic hydrolyzer, which includes β-galactosidase, coenzyme A, laccase and maltase in a mass ratio of (0.5-0.8):(0.1-0.3):(3-5):1;

[0010] The fermentation uses a composite fermentation agent, which includes xylanolytic Bacteroides, spiny Saccharopolyspora, Bacillus licheniformis and tropical Candida in a mass ratio of (0.3-0.6):(1-3):(4-6):1.

[0011] At present, most natural ingredients for skin acne removal and anti-inflammatory preparations still have unreasonable compatibility, resulting in the inability of the effective ingredients to fully exert their effects, and thus the skin acne removal and anti-inflammatory effects are poor. In response to this problem, the present invention specifically combines five Chinese medicinal raw materials, red peony root, seaweed, honeysuckle, dandelion and rose, to further improve the skin acne removal effect. Among them, red peony root has the effects of promoting blood circulation, removing blood stasis, clearing heat and cooling blood, and can be used to improve blood circulation and reduce inflammatory responses caused by blood stasis; seaweed is rich in minerals, vitamins and antioxidants, which help maintain the moisture balance of the skin and prevent skin problems caused by dryness. It also has anti-inflammatory properties, which can reduce skin redness and swelling and promote the healing of damaged skin. Iodine and other ingredients in seaweed may also help control oil secretion, thereby reducing the occurrence of acne; honeysuckle contains antiviral, antibacterial and anti-inflammatory ingredients, which can help reduce skin inflammation caused by bacterial infections; dandelion is rich in nutrients, including choline, iron, potassium, etc. These ingredients can help cleanse the blood, promote body detoxification, and have anti-inflammatory effects, which can help reduce skin redness and pain; rose extract contains a variety of antioxidants, such as vitamins C and E, as well as anti-inflammatory ingredients, which can help protect the skin from free radical damage and help maintain the skin's pH balance, thereby reducing the chance of acne formation. The five Chinese medicinal raw materials in the present invention also have a synergistic effect. The anti-inflammatory effects of honeysuckle and dandelion can enhance each other, the moisturizing property of seaweed can relieve dryness caused by inflammation, and the soothing effect of rose combined with the blood-activating effect of red peony root can help relieve skin inflammation while promoting blood circulation, thereby accelerating the skin repair process.

[0012] Although the reasonable compatibility of the above-mentioned Chinese medicine raw materials can improve the acne removal and anti-inflammatory effects of the preparation, the extracts of the Chinese medicine raw materials are usually mixtures, and the stability of the mixture is poor. After being further prepared into preparations, when placed in the air and the temperature changes, the preparations often change in appearance, affecting the use, and also affecting the acne removal and anti-inflammatory effects, which is very unfavorable for the long-term use and storage of the preparations. In response to this problem, the present invention sequentially enzymolyzes and ferments the Chinese medicine raw materials, which not only further improves the acne removal and anti-inflammatory effects of the preparations, but also improves the stability of the preparations in the air and when the temperature changes. In the enzymatic hydrolysis process, the present invention uses four enzymes, namely, β-galactosidase, coenzyme A, laccase and maltase, to enzymatically hydrolyze the Chinese medicine raw material. β-galactosidase can hydrolyze β-galactosidic bonds to release free galactose and other small molecules. β-galactosidase can help release more active ingredients, which may have anti-inflammatory and acne-removing effects, and can also promote the effects of other enzymes, because the decomposed products may be more easily processed by other enzymes. Coenzyme A is not a direct enzyme, but it participates in the metabolic process as a cofactor of many enzymatic reactions, helps to promote certain metabolic pathways, especially those involving fatty acid decomposition. In the process, coenzyme A may help improve the efficiency of enzymolysis and promote the release of active ingredients; laccase is a polyphenol oxidase that can oxidize polyphenol compounds in plants. These compounds are usually antioxidants and help improve the antioxidant capacity of the preparation. In addition, laccase can also change the structure of certain components through oxidation reactions, making them more easily absorbed by the skin, thereby enhancing the anti-inflammatory and acne-removing effects; maltase can hydrolyze starch into maltose or other smaller sugars. By decomposing the starch in the plant, maltase can release more small molecule sugars, which may serve as energy substances to support the activity of other enzymes, and may also serve as carriers to help other active ingredients be better absorbed by the skin. The present invention uses four enzymes to enzymolyze the Chinese medicine raw materials. The enzymolysis process may cause the macromolecular structure in the Chinese medicine raw materials to change, making the preparation product more stable and not easily affected by temperature changes. More antioxidant components, such as phenolic compounds, can also be released. These components can help resist oxidative stress, thereby improving the stability of the preparation in the air.

[0013] During the fermentation process, the present invention uses four strains of xylan-soluble Bacteroides, spiny polyspora, Bacillus licheniformis and tropical Candida to ferment the enzymolysis product. During the fermentation process, the strain may produce a variety of antioxidant substances, such as vitamin E, polyphenols, etc. These substances can enhance the antioxidant capacity of the preparation, thereby improving its air stability, and the strain may change the chemical structure of the enzymolysis product during the fermentation process to generate more stable compounds, thereby improving the stability of the preparation at different temperatures. Using multiple strains to ferment simultaneously can not only increase the stability of the preparation, but also further improve the anti-inflammatory effect of acne removal, because multiple active metabolites can be produced by fermentation of multiple strains, such as organic acids, antibiotics, etc., and these metabolites help to enhance the anti-inflammatory and acne removal effects of the preparation. At the same time, these four bacteria also have synergistic effects during the fermentation process, and the four strains may have different metabolic pathways during the metabolic process. The interaction between them can supplement each other's metabolic defects and jointly promote the generation of beneficial metabolites, wherein xylan-soluble Bacteroides and Bacillus licheniformis are good at decomposing complex carbohydrates, and tropical Candida may be better at converting carbohydrates into useful metabolites. These strains may create an environment that is conducive to each other's growth during the fermentation process, such as adjusting pH value, producing antibacterial substances, etc., thereby enhancing the stability and efficiency of the entire fermentation system.

[0014] Preferably, the enzymatic hydrolysis process of the Chinese medicine raw material is:

[0015] S11: Weigh the Chinese medicine raw materials according to proportion, dry and crush them to obtain Chinese medicine powder;

[0016] S12: adding water to the Chinese medicine powder, stirring evenly, and continuing to add a surfactant to obtain a suspension;

[0017] S13: adding a composite enzymatic hydrolyzing agent to the suspension, and pre-enzymolyzing for 10 to 15 hours at 37 to 45° C. to obtain a pre-enzymatic hydrolyzing solution;

[0018] S14: treating the pre-enzyme hydrolysis solution with a pulse electric field to obtain an enzymatic hydrolysis solution;

[0019] S15: filtering the enzymatic hydrolysate to obtain a supernatant, and freeze-drying the supernatant to obtain an enzymatic hydrolysate.

[0020] The present invention has further studied and found that although the use of four enzymes and four bacteria to perform enzymolysis and fermentation on Chinese medicine raw materials can improve the high and low temperature stability and antioxidant capacity of the preparation under air, it will still deteriorate in appearance to a certain extent and reduce the anti-acne and anti-inflammatory effects under light irradiation. In response to this problem, the present invention has further studied the enzymolysis process of Chinese medicine raw materials and found that the addition of surfactants and enzymolysis in conjunction with pulsed electric field treatment can further improve the light resistance of the preparation. Analysis of the reasons: Through enzymolysis and pulsed electric field treatment, the antioxidant components in the plants, such as polyphenols, flavonoids, etc., can be more effectively released. These components themselves have the ability to resist ultraviolet rays, thereby improving the photostability of the preparation.

[0021] Preferably, in step S12, the mass ratio of the Chinese medicine powder to water is 1:(8-12), and the mass ratio of the Chinese medicine powder to the surfactant is 1:(0.01-0.05).

[0022] Preferably, in step S12, the surfactant includes Span-40, Tween-20 and polyethylene glycol in a mass ratio of (0.3-0.5):(0.8-1):1.

[0023] Preferably, in step S13, the mass ratio of the Chinese medicine powder to the composite enzymatic hydrolyzing agent is 1:(0.05-0.08).

[0024] Preferably, in step S14, the electric field intensity of the pulse electric field treatment is 2-3 kV / cm, the number of pulses is 15-30 times, the pulse width is 10-20 μs, the frequency is 3-5 Hz, and the treatment time is 60-100 s.

[0025] Preferably, the fermentation process of the enzymolysis product is:

[0026] S21: adding a composite fermentation agent to the enzymatic hydrolyzate, then adding water, introducing oxygen, and fermenting at 25 to 35° C. for 7 to 15 days to obtain a fermentation liquid;

[0027] S22: filtering the fermentation liquid to obtain a filtrate, sterilizing the filtrate to inactivate the bacteria;

[0028] S23: centrifuging the sterilized filtrate, collecting the supernatant, and freeze-drying it to obtain the acne-removing and anti-inflammatory ingredients.

[0029] Preferably, in step S21, the inoculation amount of the composite fermentation agent is 3-6%, and the amount of water added is 20-25 times the amount of the enzymatic hydrolysis product;

[0030] In step S22, sterilize at 100-120°C for 30-40 minutes.

[0031] Preferably, the emollient is at least one of butylene glycol, hexylene glycol, pentylene glycol, behenyl alcohol, caprylyl glycol, caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, olive oil, dimethiconol, isohexadecane, phytosterol, polydecene, and isononyl isononanoate;

[0032] The emulsifier is at least one of polysorbate-20, laureth-23, sorbitan olivate, steareth-21, polysorbate-80, soy lecithin PC60, and glyceryl stearate;

[0033] The filler is at least one of boron nitride, diatomaceous earth, kaolin, cellulose acetate, and starch octyl aluminum succinate;

[0034] The thickener is at least one of glycerol, 1,3-propylene glycol, carbomer, xanthan gum, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, gum arabic, polymer EMT-10, sodium acrylate / sodium acryloyldimethyl taurate, and acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer copolymer;

[0035] The preservative is at least one of phenoxyethanol, ethylhexylglycerin, phenoxyethanol, sodium benzoate, pentylene glycol and hexylene glycol;

[0036] The pH adjuster is at least one of lactic acid and arginine.

[0037] The second aspect of the present invention provides a method for preparing a skin acne-removing and anti-inflammatory preparation, comprising the following steps:

[0038] S1: Disperse the thickener completely in water to obtain a mixed solution;

[0039] S2: adding anti-acne and anti-inflammatory ingredients, emollients, fillers, preservatives and pH adjusters to the mixed solution in step S1, heating to 65° C., and homogenizing at 3000 r / min for 3 minutes to fully disperse the mixture;

[0040] S3: Add emulsifier and essence to step S2, homogenize at 3000 r / min for 3 minutes to fully emulsify it, heat to 65° C., and obtain a skin acne-removing and anti-inflammatory preparation.

[0041] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0042] 1. The present invention adopts the process of enzymatic hydrolysis and fermentation. By using a variety of enzymes and a variety of bacterial species, it can more effectively decompose the complex macromolecules in the plant raw materials, thereby improving the release efficiency of the active ingredients and improving the acne-removing and anti-inflammatory effects of the preparation.

[0043] 2. The use of surfactants in the present invention helps to improve the efficiency of enzymatic hydrolysis, while pulsed electric field treatment can further destroy the cell wall and release more active substances, thereby enhancing the stability and bioavailability of the preparation.

[0044] 3. The metabolites produced during the enzymatic hydrolysis and fermentation process of the present invention can improve the physical and chemical stability of the preparation, wherein the increase of antioxidant components helps to resist the influence of external factors (such as light and heat) on the preparation, thereby improving its stability during storage and use. DETAILED DESCRIPTION

[0045] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] Example 1

[0047] This embodiment provides a skin acne removal and anti-inflammatory preparation, the composition of which is shown in Table 1.

[0048] Table 1 Preparation composition of Example 1

[0049]

[0050]

[0051] The anti-acne and anti-inflammatory ingredients are obtained by enzymatic hydrolysis and fermentation of traditional Chinese medicine raw materials. The specific preparation process is as follows:

[0052] (1) The enzymatic hydrolysis process of Chinese medicine raw materials is:

[0053] S11: Weigh the Chinese medicine raw materials according to proportion, dry and crush them to obtain Chinese medicine powder;

[0054] The Chinese medicinal materials include red peony root, seaweed, honeysuckle, dandelion and rose in a mass ratio of 5:2:7:11:1;

[0055] S12: adding water to the Chinese medicine powder, stirring evenly, and continuing to add a surfactant to obtain a suspension; the mass ratio of the Chinese medicine powder to water is 1:10, the mass ratio of the Chinese medicine powder to the surfactant is 1:0.03, and the surfactant includes Span-40, Tween-20 and polyethylene glycol (CAS: 25322-68-3) in a mass ratio of 0.4:0.9:1;

[0056] S13: adding a composite enzymatic hydrolyzer to the suspension, and performing pre-enzymatic hydrolysis at 40° C. for 12 hours to obtain a pre-enzymatic hydrolyzer solution; the mass ratio of the Chinese medicine powder to the composite enzymatic hydrolyzer is 1:0.06; the composite enzymatic hydrolyzer includes β-galactosidase, coenzyme A, laccase and maltase in a mass ratio of 0.6:0.2:4:1;

[0057] β-Galactosidase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10066;

[0058] Coenzyme A was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10106;

[0059] Laccase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10189;

[0060] Maltase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10191;

[0061] S14: subjecting the pre-enzyme hydrolysis solution to a pulse electric field treatment to obtain an enzymatic hydrolysis solution; the electric field intensity of the pulse electric field treatment is 2.5 kV / cm, the number of pulses is 20 times, the pulse width is 15 μs, the frequency is 4 Hz, and the treatment time is 80 s;

[0062] S15: performing enzyme inactivation treatment on the enzymatic hydrolysate, filtering to obtain a supernatant, and freeze-drying the supernatant to obtain an enzymatic hydrolysate.

[0063] (2) The fermentation process of the enzymatic hydrolysis product is:

[0064] S21: adding a composite fermentation agent to the enzymatic hydrolysate, then adding water, sealing, and fermenting at 30° C. for 8 days to obtain a fermentation liquid; the inoculation amount of the composite fermentation agent is 5%, and the amount of water added is 20 times the amount of the enzymatic hydrolysate; the composite fermentation agent includes xylanolytic Bacteroides, spiny polyspora, Bacillus licheniformis and tropical Candida in a mass ratio of 0.4:2:5:1;

[0065] Bacteroides xylanolyticus was purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC353923;

[0066] Saccharopolyspora spinosa was purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC153056;

[0067] Bacillus licheniformis was purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC336463;

[0068] Candida tropicalis was purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC334135;

[0069] S22: filtering the fermentation broth to obtain a filtrate, sterilizing the filtrate to inactivate the bacteria; sterilizing at 105° C. for 35 minutes;

[0070] S23: centrifuging the sterilized filtrate, collecting the supernatant, and freeze-drying it to obtain the acne-removing and anti-inflammatory ingredients.

[0071] A method for preparing a skin acne-removing and anti-inflammatory preparation, specifically a method for preparing a skin acne-removing and anti-inflammatory essence lotion, comprising the following steps:

[0072] S1: Disperse the thickener completely in water to obtain a mixed solution;

[0073] S2: adding anti-acne and anti-inflammatory ingredients, emollients, fillers, preservatives and pH adjusters to the mixed solution in step S1, heating to 65° C., and homogenizing at 3000 r / min for 3 minutes to fully disperse the mixture;

[0074] S3: Add emulsifier and essence to step S2, homogenize at 3000 r / min for 3 minutes to fully emulsify it, heat to 65° C., and obtain a skin acne-removing and anti-inflammatory preparation.

[0075] Example 2

[0076] The difference between this embodiment and embodiment 1 is that the added amount of the acne-removing and anti-inflammatory ingredients is 18%.

[0077] Comparative Example 1

[0078] The difference between this comparative example and Example 1 is that the mass ratio of red peony root, seaweed, honeysuckle, dandelion and rose is 10:2:7:11:1.

[0079] Comparative Example 2

[0080] The difference between this comparative example and Example 1 is that no surfactant is added during the enzymatic hydrolysis of the Chinese medicine raw material. The enzymatic hydrolysis process of the Chinese medicine raw material in this comparative example is as follows:

[0081] S11: Weigh the Chinese medicine raw materials according to proportion, dry and crush them to obtain Chinese medicine powder;

[0082] The Chinese medicinal materials include red peony root, seaweed, honeysuckle, dandelion and rose in a mass ratio of 5:2:7:11:1;

[0083] S12: adding water to the Chinese medicine powder and stirring evenly to obtain a suspension; the mass ratio of the Chinese medicine powder to water is 1:10;

[0084] S13: adding a composite enzymatic hydrolyzer to the suspension, and performing pre-enzymatic hydrolysis at 40° C. for 12 hours to obtain a pre-enzymatic hydrolyzer solution; the mass ratio of the Chinese medicine powder to the composite enzymatic hydrolyzer is 1:0.06; the composite enzymatic hydrolyzer includes β-galactosidase, coenzyme A, laccase and maltase in a mass ratio of 0.6:0.2:4:1;

[0085] S14: subjecting the pre-enzyme hydrolysis solution to a pulse electric field treatment to obtain an enzymatic hydrolysis solution; the electric field intensity of the pulse electric field treatment is 2.5 kV / cm, the number of pulses is 20 times, the pulse width is 15 μs, the frequency is 4 Hz, and the treatment time is 80 s;

[0086] S15: performing enzyme inactivation treatment on the enzymatic hydrolysate, filtering to obtain a supernatant, and freeze-drying the supernatant to obtain an enzymatic hydrolysate.

[0087] Comparative Example 3

[0088] The difference between this comparative example and Example 1 is that the pulse electric field treatment is not performed during the enzymatic hydrolysis of the Chinese medicine raw material. The specific enzymatic hydrolysis process of the Chinese medicine raw material in this comparative example is as follows:

[0089] S11: Weigh the Chinese medicine raw materials according to proportion, dry and crush them to obtain Chinese medicine powder;

[0090] The Chinese medicinal materials include red peony root, seaweed, honeysuckle, dandelion and rose in a mass ratio of 5:2:7:11:1;

[0091] S12: adding water to the Chinese medicine powder, stirring evenly, and continuing to add a surfactant to obtain a suspension; the mass ratio of the Chinese medicine powder to water is 1:10, the mass ratio of the Chinese medicine powder to the surfactant is 1:0.03, and the surfactant includes Span-40, Tween-20 and polyethylene glycol in a mass ratio of 0.4:0.9:1;

[0092] S13: adding a composite enzymatic hydrolyzer to the suspension, and performing pre-enzymatic hydrolysis at 40° C. for 12 hours to obtain a pre-enzymatic hydrolyzer solution; the mass ratio of the Chinese medicine powder to the composite enzymatic hydrolyzer is 1:0.06; the composite enzymatic hydrolyzer includes β-galactosidase, coenzyme A, laccase and maltase in a mass ratio of 0.6:0.2:4:1;

[0093] S14: The pre-enzymatic solution is subjected to enzyme inactivation treatment, and then filtered to obtain a supernatant, and the supernatant is freeze-dried to obtain an enzymatic hydrolysis product.

[0094] Comparative Example 4

[0095] The difference between this comparative example and Example 1 is that the mass ratio of β-galactosidase, coenzyme A, laccase and maltase is 0.6:1:4:1.

[0096] Comparative Example 5

[0097] The difference between this comparative example and Example 1 is that coenzyme A is replaced by coenzyme Q10, which is purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10105.

[0098] Comparative Example 6

[0099] The difference between this comparative example and Example 1 is that maltase is replaced by sucrase, which is purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10208.

[0100] Comparative Example 7

[0101] The difference between this comparative example and Example 1 is that the mass ratio of Span-40, Tween-20 and polyethylene glycol is 1:0.9:1.

[0102] Comparative Example 8

[0103] The difference between this comparative example and Example 1 is that Tween-20 is replaced by Tween-80.

[0104] Comparative Example 9

[0105] The difference between this comparative example and Example 1 is that the mass ratio of Bacteroides xylanolyticus, Saccharopolyspora spinosa, Bacillus licheniformis and Candida tropicalis is 1:2:5:1.

[0106] Comparative Example 10

[0107] The difference between this comparative example and Example 1 is that Bacillus licheniformis is replaced by Bacillus cereus purchased from Henan Industrial Microbial Strain Engineering Technology Research Center, model number BNCC103930.

[0108] Comparative Example 11

[0109] The difference between this comparative example and Example 1 is that tropical Candida is replaced by Saccharomyces cerevisiae, which is purchased from Henan Industrial Microbial Strain Engineering Technology Research Center with model number BNCC336054.

[0110] Performance Testing

[0111] (1) Safety testing

[0112] Test method: Use a third-party testing agency to test safety. Select an area not exceeding 50mm 2 , a suitable patch with a depth of about 1 mm, and about 0.02 g of the test substance is added to the patch tester by a closed patch test method. The patch tester is attached to the curved side of the forearm of the subject, and the test substance is removed after 24 hours. The skin reaction is observed 48 hours after removal, and the results are recorded according to the skin reaction classification standard in the "Cosmetic Safety Technical Regulations 2015". The test results of the comparative examples and embodiments are shown in Table 2.

[0113] The skin reaction grading standards are as follows:

[0114] Grade 0: negative reaction;

[0115] Grade 1: Suspicious reaction; only slight erythema;

[0116] Grade 2: weak positive reaction (erythema reaction); erythema, infiltration, edema, and papules may be present;

[0117] Grade 3: Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, herpes; the reaction may extend beyond the test area;

[0118] Grade 4: Very strong reaction (confluent herpes reaction); obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0119] Table 2 Safety test results

[0120]

[0121] As can be seen from Table 2, the preparations prepared in the examples and comparative examples are relatively safe and can be used for subsequent experiments.

[0122] (2) Stability test

[0123] Test method: Internal control standard, observe the appearance color for three months.

[0124] The preparations prepared in Examples and Comparative Examples were placed at 45° C. for 14 days, and their appearances were observed.

[0125] The preparations prepared in Examples and Comparative Examples were placed at -15°C for 14 days, and their appearances were observed.

[0126] After the preparations prepared in Examples and Comparative Examples were placed under light for 14 days, their appearances were observed.

[0127] After the above experiment, the appearance test results are expressed as 1, 2 and 3, where 1 means no obvious change in appearance, 2 means a small amount of material precipitates, and 3 means a large amount of material precipitates. The test results are shown in Table 3.

[0128] Table 3 Stability test results

[0129]

[0130] As can be seen from Table 3, the preparations of Examples 1-2 can maintain their appearance stability under high temperature (45°C), low temperature (-15°C) and light, while Comparative Examples 1-11 all have appearance precipitation problems to varying degrees and have poor stability.

[0131] (3) Evaluation of acne-removing efficacy

[0132] Test method: Twice a day, morning and evening, apply 0.5g of the preparation prepared in the embodiment and comparative example on the entire face. The facial condition was analyzed before using the sample (D0) and 28 days after using the sample (D28). Primos was used for facial image acquisition and acne analysis. Skin acne volume change rate = (skin acne volume before using the sample - skin acne volume after using the sample) / skin acne volume before using the sample × 100. The test results are shown in Table 4.

[0133] Table 4 Acne removal test results

[0134]

[0135] It can be seen from the above performance test results that Examples 1-2 have good acne removal effects and stability, especially Example 1 has the most outstanding comprehensive performance. This is mainly because the present invention adds acne removal and anti-inflammatory ingredients, the overall formula is reasonable, and acne skin is conditioned in an all-round way.

[0136] The comparative example is not adopted the necessary technical solution, which leads to the corresponding performance test being significantly worse than the embodiment. The ratio of Chinese medicine raw materials is changed in comparative example 1. It can be seen that the anti-acne effect decreases, proving that the ratio of Chinese medicine raw materials has an important influence on the anti-acne effect and stability effect of the preparation. In comparative example 2, no surfactant is used in the enzymolysis process. It can be seen from the results that the extraction process of Chinese medicine raw material components is affected, further resulting in a decrease in acne and stability effects. In comparative example 3, the pre-enzyme hydrolyzate is not subjected to pulse electric field treatment. It can be seen from the results that the anti-acne effect and stability of the preparation prepared by it are both decreased, indicating that the present invention adds surfactants and performs pulse electric field treatment during the enzymolysis of Chinese medicine raw materials. It has an important influence on improving the anti-acne effect and stability of the preparation, and both are indispensable. Comparative example 4 changes the ratio of four enzymes. It can be seen from the results that the anti-acne effect and preparation stability of its preparation decrease, indicating that the ratio between multiple enzymes has a certain degree of influence on improving the comprehensive performance of the preparation. Comparative Examples 5-6 respectively changed the types of two enzymes. It can be seen from the results that the acne-removing effect and stability of the preparations prepared therefrom further decreased, indicating that the present invention uses a specific type of enzyme to combine and enzymolyze the Chinese medicine raw materials, which has an important influence on improving the comprehensive performance of the preparation. Comparative Examples 7 and 8 respectively changed the ratio and type of surfactants. It can be seen from the results that the comprehensive performance of the preparation decreased, indicating that the type and ratio of surfactants have a certain degree of influence on the acne-removing effect and stability of the preparation. Comparative Example 9 changed the ratio of four fermented bacteria. It can be seen from the results that the acne-removing effect and stability of its preparation decreased. Comparative Examples 10-11 respectively changed the types of two fermented bacteria. It can be seen from the results that the acne-removing effect and stability of its preparations further decreased, indicating that the mixing ratio of the fermented bacteria and the type of the fermented bacteria in the present invention have a very important influence on improving the comprehensive performance of the preparation. The above experimental results further prove the importance of the technical scheme defined in the present invention for its technical effect.

[0137] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A skin acne and anti-inflammatory preparation, characterized in that: The following raw materials are included in percentage by mass: 15-30% of acne-removing and anti-inflammatory ingredients, 0.5-20% of moisturizer, 0.5-15% of emulsifier, 1-25% of filler, 1-15% of thickener, 0.05-0.3% of preservative, 0.01-0.5% of pH adjuster, 0.005-0.05% of flavor and the balance of water; The anti-acne and anti-inflammatory ingredients are obtained by enzymatic hydrolysis and fermentation of traditional Chinese medicine raw materials; The Chinese medicinal materials include red peony root, seaweed, honeysuckle, dandelion and rose in a mass ratio of (4-6):(1-3):(6-8):(10-12):1; The enzymatic hydrolysis uses a composite enzymatic hydrolyzer, which includes β-galactosidase, coenzyme A, laccase and maltase in a mass ratio of (0.5-0.8):(0.1-0.3):(3-5):1; The fermentation uses a composite fermentation agent, which includes xylanolytic Bacteroides, spiny Saccharopolyspora, Bacillus licheniformis and tropical Candida in a mass ratio of (0.3-0.6):(1-3):(4-6):

1.

2. A skin acne-removing and anti-inflammatory preparation according to claim 1, characterized in that: The enzymatic hydrolysis process of Chinese medicine raw materials is: S11: Weigh the Chinese medicine raw materials according to proportion, dry and crush them to obtain Chinese medicine powder; S12: adding water to the Chinese medicine powder, stirring evenly, and continuing to add a surfactant to obtain a suspension; S13: adding a composite enzymatic hydrolyzing agent to the suspension, and pre-enzymolyzing at 37-45° C. for 10-15 hours to obtain a pre-enzymatic hydrolyzing solution; S14: treating the pre-enzyme hydrolysis solution with a pulse electric field to obtain an enzymatic hydrolysis solution; S15: filtering the enzymatic hydrolysate to obtain a supernatant, and freeze-drying the supernatant to obtain an enzymatic hydrolysate.

3. A skin acne-removing and anti-inflammatory preparation according to claim 2, characterized in that: In step S12, the mass ratio of the Chinese medicine powder to water is 1:(8-12), and the mass ratio of the Chinese medicine powder to the surfactant is 1:(0.01-0.05).

4. The skin acne-removing and anti-inflammatory preparation according to claim 2, characterized in that: In step S12, the surfactant includes Span-40, Tween-20 and polyethylene glycol in a mass ratio of (0.3-0.5):(0.8-1):

1.

5. The skin acne-removing and anti-inflammatory preparation according to claim 2, characterized in that: In step S13, the mass ratio of the Chinese medicine powder to the composite enzymatic hydrolyzing agent is 1:(0.05-0.08).

6. The skin acne-removing and anti-inflammatory preparation according to claim 2, characterized in that: In step S14, the electric field intensity of the pulse electric field treatment is 2-3 kV / cm, the number of pulses is 15-30 times, the pulse width is 10-20 μs, the frequency is 3-5 Hz, and the treatment time is 60-100 s.

7. The skin acne-removing and anti-inflammatory preparation according to claim 2, characterized in that: The fermentation process of enzymatic hydrolysis products is as follows: S21: adding a composite fermentation agent to the enzymatic hydrolyzate, then adding water, introducing oxygen, and fermenting at 25 to 35° C. for 7 to 15 days to obtain a fermentation liquid; S22: filtering the fermentation liquid to obtain a filtrate, sterilizing the filtrate to inactivate the bacteria; S23: centrifuging the sterilized filtrate, collecting the supernatant, and freeze-drying it to obtain the acne-removing and anti-inflammatory ingredients.

8. The skin acne-removing and anti-inflammatory preparation according to claim 7, characterized in that: In step S21, the inoculation amount of the composite leavening agent is 3-6%, and the amount of water added is 20-25 times of the enzymatic hydrolysis product; in step S22, sterilization is performed at 100-120° C. for 30-40 minutes.

9. The skin acne-removing and anti-inflammatory preparation according to claim 1, characterized in that: The emollient is at least one of butylene glycol, hexylene glycol, pentylene glycol, behenyl alcohol, caprylyl glycol, caprylic capric triglyceride, squalane, avocado oil, shea butter, jojoba oil, olive oil, dimethiconol, isohexadecane, phytosterol, polydecene, and isononyl isononanoate; The emulsifier is at least one of polysorbate-20, laureth-23, sorbitan olivate, steareth-21, polysorbate-80, soy lecithin PC60, and glyceryl stearate; The filler is at least one of boron nitride, diatomaceous earth, kaolin, cellulose acetate, and starch octyl aluminum succinate; The thickener is at least one of glycerol, 1,3-propylene glycol, carbomer, xanthan gum, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, gum arabic, polymer EMT-10, sodium acrylate / sodium acryloyldimethyl taurate, and acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer copolymer; The preservative is at least one of phenoxyethanol, ethylhexylglycerin, phenoxyethanol, sodium benzoate, pentylene glycol and hexylene glycol; The pH adjuster is at least one of lactic acid and arginine.

10. A method for preparing a skin acne-removing and anti-inflammatory preparation according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1: Disperse the thickener completely in water to obtain a mixed solution; S2: adding anti-acne and anti-inflammatory ingredients, emollients, fillers, preservatives and pH adjusters to the mixed solution in step S1, heating to 65° C., and homogenizing at 3000 r / min for 3 minutes to fully disperse the mixture; S3: Add emulsifier and essence to step S2, homogenize at 3000 r / min for 3 minutes to fully emulsify it, heat to 65° C., and obtain a skin acne-removing and anti-inflammatory preparation.

Images