Storage device for chitosan oligosaccharide cultivation

Abstract

The invention relates to the technical field of chitosan oligosaccharide cultivation, in particular to a storage device for chitosan oligosaccharide cultivation. The invention provides a storage device for cultivating chitosan oligosaccharide, which can simultaneously stir and dissolve a plurality of parts of chitosan so as to compare the enzymolysis effects of different enzyme preparations and select the optimal chitosan oligosaccharide. A storage device for chitosan oligosaccharide cultivation comprises a base, a storage mechanism and a slow stirring mechanism, the storage mechanism capable of storing reaction liquid is arranged on the base, and the slow stirring mechanism capable of stirring the reaction liquid is further arranged on the base. A central motor is started to drive a second rotating shaft to rotate, a belt rotates to drive a rotating sleeve to rotate, a long rod rotates to stir a solution in a splicing disc, and after chitosan is dissolved, different enzyme preparations are added into the splicing disc, so that multiple parts of chitosan can be stirred and dissolved at the same time; therefore, the optimal effect can be selected by comparing the enzymolysis effects of different enzyme preparations.

Description

Technical Field

[0001] The invention relates to the technical field of chitosan oligosaccharide cultivation, in particular to a storage device for chitosan oligosaccharide cultivation. Background Art

[0002] Chitosan oligosaccharides are oligosaccharides obtained by partial hydrolysis of chitosan, and have multiple biological activities and application values. Enzymatic hydrolysis is a method of generating chitosan oligosaccharides by catalyzing the hydrolysis of chitosan with specific enzymes. At present, when chitosan oligosaccharides are obtained by enzymatic hydrolysis, chitosan is added to a prepared aqueous solution, stirred under certain temperature conditions to dissolve the chitosan, and then an enzyme preparation liquid is added to allow it to be enzymolyzed at a constant temperature for a certain period of time, and then the reaction liquid is filtered to remove impurities, thereby obtaining chitosan oligosaccharides. Since the effects of different enzyme preparations may be different, when comparing the enzymatic hydrolysis effects of different enzyme preparations, it is necessary to stir and dissolve multiple portions of chitosan at the same time, and then add different enzyme preparations to compare the enzymatic hydrolysis effects, which is labor-intensive and inconvenient to operate.

[0003] Therefore, a storage device capable of simultaneously stirring and dissolving multiple portions of chitosan has been developed so as to compare the enzymolysis effects of different enzyme preparations and select the best chitosan oligosaccharide cultivation device. Summary of the invention

[0004] In order to overcome the disadvantages of needing to stir and dissolve multiple portions of chitosan at the same time and then add different enzyme preparations to compare the enzymatic hydrolysis effects when comparing the enzymatic hydrolysis effects of different enzyme preparations, which is labor-intensive and inconvenient to operate, the present invention provides a storage device for chitosan oligosaccharide cultivation that can stir and dissolve multiple portions of chitosan at the same time, so as to compare the enzymatic hydrolysis effects of different enzyme preparations and select the optimal one.

[0005] Technical solution: A storage device for chitosan oligosaccharide cultivation includes a base, a storage mechanism and a slow stirring mechanism. The base is provided with a storage mechanism capable of storing a reaction liquid, and the base is also provided with a slow stirring mechanism capable of stirring the reaction liquid.

[0006] In addition, it is particularly preferred that the storage mechanism includes an outer box, a first rotating shaft, a door panel, a bracket, a layering tray, a middle rack and a splicing tray, the outer box is connected to the upper side of the base, the first rotating shaft is rotatably connected to the left and right sides of the front of the outer box, the door panel is connected to the first rotating shaft, the bracket is connected to the upper middle side of the base, a plurality of layering trays are connected to the bracket, the middle rack is clamped on the layering tray, and the splicing trays are connected to the left and right sides of the middle rack.

[0007] Furthermore, it is particularly preferred that each door panel is provided with a handle.

[0008] In addition, it is particularly preferred that the slow stirring mechanism includes a central motor, a second rotating shaft, a connecting frame, a fixed cover, a support plate, a rotating sleeve, a belt, a long rod and a knocking assembly. The central motor is connected to the lower side of the middle part of the base, and the second rotating shaft is connected to the output shaft of the central motor. The second rotating shaft passes through the bracket and is rotatably connected to the outer box. The upper part of the layered disk is connected to a connecting frame, and the connecting frame is connected to a fixed cover. A plurality of support plates are connected to the left and right sides of the bracket, and the support plates are rotatably connected with a rotating sleeve. A belt is wrapped around two laterally adjacent rotating sleeves, and the belt is in contact with the second rotating shaft. The diameter of the second rotating shaft is larger than that of the rotating sleeve. Long rods are slidably connected to the rotating sleeves, and the long rods pass through adjacent fixed covers. A knocking assembly is provided between the second rotating shaft and the outer box. The central motor is started to drive the second rotating shaft to rotate, so that the belt rotation drives the rotating sleeve on the support plate to rotate, and the long rod rotates to stir the solution to dissolve the chitosan.

[0009] In addition, it is particularly preferred that the knocking assembly includes a screw ball and a knocking ball, a plurality of screw balls are threadedly connected to the upper portion of the second rotating shaft, and a plurality of knocking balls are connected to the inner side of the upper portion of the outer box. When the second rotating shaft rotates, the screw ball is driven to rotate, so that the screw ball hits the knocking ball, causing the outer box to vibrate, and then causing the splicing plate to vibrate, further improving the solution stirring efficiency.

[0010] In addition, it is particularly preferred that a locking mechanism is also included, the locking mechanism including an elastic locking plate, a retraction buckle, a synchronous plate, a back pressure plate, a guide rail and a rotating plate, a plurality of elastic locking plates are connected to the inner side of the rear portion of the outer box, a plurality of retraction buckles are slidably connected to the rear side of the outer box, the retraction buckles are all in contact with adjacent elastic locking plates, a synchronous plate is connected between the rear portions of the retraction buckles, a back pressure plate is connected to the synchronous plate, the front and rear guide rails are connected to the left and right sides of the middle portion of the outer box, the guide rails are slidably connected to the back pressure plate, the middle of the first rotating shaft is connected to a rotating plate, and the rotating plate is pressed and matched with the back pressure plate. When the door panel is opened, the first rotating shaft drives the rotating plate to rotate and squeeze the back pressure plate, so that the back pressure plate moves backward along the guide rail, so that the synchronous plate and the retraction buckle move backward, and when the retraction buckle moves backward, the elastic locking plate is squeezed inward and deformed, and after the middle frame is pushed into the layered disk to close the door panel, the rotating plate disengages from the back pressure plate, so that the elastic locking plate resumes the card-engagement with the middle frame.

[0011] In addition, it is particularly preferred that a lower locking mechanism is also included, and the lower locking mechanism includes a compression spring and an inclined fork plate. The rear sides of the left and right parts of the outer box are slidably connected with multiple inclined fork plates, and the inclined fork plates are connected to the outer box with compression springs. The long rods are slidably matched with adjacent inclined fork plates. When the door panel is closed, the inclined fork plates are squeezed to move backward, and the compression springs are squeezed and contracted, so that the long rods move downward along the inclined fork plates and are immersed in the solution. After the door panel is opened, the compression springs rebound, so that the inclined fork plates move forward and reset, and then the long rods move upward along the inclined fork plates and are lifted.

[0012] In addition, it is particularly preferred that a ventilation mechanism is also included, which includes an outer cylinder and a fan. The upper sides of the left and right parts of the outer box are connected to the outer cylinders, and fans are installed on the outer cylinders. When the interior of the outer box needs to be ventilated, the fan on the outer cylinder is started.

[0013] In addition, it is particularly preferred that a temperature control mechanism is also included, the temperature control mechanism includes a temperature controller and a temperature control tube, the left and right sides of the upper part of the outer box are connected to the temperature controller, and multiple temperature control tubes are connected to the temperature controller, and the temperature control tubes all pass through the outer box. When chitosan is enzymatically hydrolyzed in the splicing disk, the temperature controller is started according to the enzymatic hydrolysis requirements, so that the temperature control tubes adjust the temperature inside the outer box.

[0014] In addition, it is particularly preferred that a display screen is provided on the temperature controller.

[0015] The beneficial effects are as follows: 1. The present invention starts the central motor to drive the second rotating shaft to rotate, so that the belt rotates to drive the rotating sleeve to rotate, so that the long rod rotates to stir the solution in the splicing disk. After the chitosan is dissolved, different enzyme preparations are added to the splicing disk, so that multiple portions of chitosan can be stirred and dissolved at the same time, so as to compare the enzymatic hydrolysis effects of different enzyme preparations and select the best effect.

[0016] 2. The present invention pushes the middle frame into the layered tray and closes the door panel, and the rotating plate is separated from the return pressure plate, so that the elastic locking plate is restored to be engaged with the middle frame, thereby achieving the effect of being able to lock the middle frame and prevent the middle frame from falling.

[0017] 3. When the door panel of the present invention is closed, the inclined fork plate is squeezed to move backward, and the compression spring is squeezed and contracted, so that the long rod moves downward along the inclined fork plate and is immersed in the solution. After the door panel is opened, the compression spring rebounds, so that the inclined fork plate moves forward and resets, and then the long rod moves upward along the inclined fork plate and is lifted, thereby achieving the effect of being able to automatically control the long rod and improving the convenience of operation.

[0018] 4. The present invention achieves the effect of ventilating the outer box, reducing the humidity inside the outer box, and preventing the growth of mold by starting the fan on the outer cylinder when the inside of the outer box needs to be ventilated.

[0019] 5. When chitosan is enzymolyzed in the splicing tray, the present invention starts the temperature controller according to the enzymolysis requirements, so that the temperature control tube adjusts the temperature in the outer box, thereby achieving the effect of enabling chitosan to be enzymolyzed at a suitable temperature and ensuring the enzymolysis effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0021] Figure 2 It is a partial three-dimensional structural schematic diagram of the present invention.

[0022] Figure 3 It is a schematic cross-sectional view of the three-dimensional structure of the storage mechanism of the present invention.

[0023] Figure 4 It is a schematic cross-sectional view of the three-dimensional structure of the slow stirring mechanism of the present invention.

[0024] Figure 5 It is a partial three-dimensional structural schematic diagram of the slow stirring mechanism of the present invention.

[0025] Figure 6 It is a partial three-dimensional structural schematic diagram of the slow stirring mechanism of the present invention.

[0026] Figure 7 It is a partial three-dimensional structural cross-sectional schematic diagram of the locking mechanism of the present invention.

[0027] Figure 8 It is a three-dimensional structural schematic diagram of the locking mechanism of the present invention.

[0028] Fig. 9 It is a schematic cross-sectional view of the three-dimensional structure of the lower locking mechanism of the present invention.

[0029] Fig.10 It is a three-dimensional structural schematic diagram of the ventilation mechanism and the temperature control mechanism of the present invention.

[0030] In the figure: 1. base, 2. storage mechanism, 21. outer box, 22. first rotating shaft, 23. door panel, 24. bracket, 25. layered plate, 26. middle frame, 27. splicing plate, 3. slow stirring mechanism, 31. central motor, 32. second rotating shaft, 33. connecting frame, 34. fixed cover, 35. support plate, 36. rotating sleeve, 37. belt, 38. long rod, 39. screw ball, 310. knocking ball, 4. locking mechanism, 41. elastic locking plate, 42. retraction buckle, 43. synchronous plate, 44. back pressure plate, 45. guide rail, 46. rotating plate, 51. compression spring, 5. lower locking mechanism, 52. oblique fork plate, 6. ventilation mechanism, 61. outer cylinder, 62. fan, 7. temperature control mechanism, 71. temperature controller, 72. temperature control tube. DETAILED DESCRIPTION

[0031] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0032] A storage device for chitosan oligosaccharide cultivation, such as Figure 1-Figure 10 As shown, it includes a base 1, a storage mechanism 2 and a slow stirring mechanism 3. The base 1 is provided with the storage mechanism 2, and the base 1 is also provided with the slow stirring mechanism 3.

[0033] like Figure 1 and Figure 3As shown, the storage mechanism 2 includes an outer box 21, a first rotating shaft 22, a door panel 23, a bracket 24, a layering tray 25, a middle frame 26 and a splicing tray 27. The outer box 21 is connected to the upper side of the base 1, and the first rotating shaft 22 is rotatably connected to the left and right sides of the front of the outer box 21. The first rotating shaft 22 is connected to the door panel 23, and the door panel 23 is provided with a handle for easy grasping. The bracket 24 is connected to the upper middle side of the base 1, and five layering trays 25 are connected to the bracket 24. The layering trays 25 are clamped with the middle frame 26, and the splicing trays 27 are connected to the left and right sides of the middle frame 26.

[0034] like Figure 1 , Figure 4 , Figure 5 and Figure 6 As shown, the slow stirring mechanism 3 includes a central motor 31, a second rotating shaft 32, a connecting frame 33, a fixed cover 34, a support plate 35, a rotating sleeve 36, a belt 37, a long rod 38 and a knocking assembly. The central motor 31 is connected to the lower side of the middle of the base 1, and the second rotating shaft 32 is connected to the output shaft of the central motor 31. The second rotating shaft 32 passes through the bracket 24 and is rotatably connected to the outer box 21. The upper part of the layered plate 25 is connected to the connecting frame 33, and the connecting frame 33 is connected to the fixed cover 34. The left and right sides of the bracket 24 are connected to five support plates 35, and the support plates 35 are connected to the fixed cover 34. A rotating sleeve 36 is rotatably connected, and a belt 37 is wound around two laterally adjacent rotating sleeves 36. The belt 37 is in contact with the second rotating shaft 32. The diameter of the second rotating shaft 32 is larger than that of the rotating sleeve 36. A long rod 38 is slidably connected to the rotating sleeve 36. The long rod 38 passes through the adjacent fixed cover 34. A knocking assembly is provided between the second rotating shaft 32 and the outer box 21. The knocking assembly includes a screw ball 39 and a knocking ball 310. Four screw balls 39 are threadedly connected to the upper part of the second rotating shaft 32, and four knocking balls 310 are connected to the inner side of the upper part of the outer box 21.

[0035] When using the present invention, first place the base 1 in the chitosan oligosaccharide cultivation area, then rotate and open the door panel 23 to drive the first rotating shaft 22 to rotate, then remove the middle frame 26 from the layering plate 25, then pour the prepared aqueous solution into the splicing plate 27, and add chitosan into the aqueous solution, then re-engage the middle frame 26 with the splicing plate 27 so that the fixed cover 34 on the connecting frame 33 covers the splicing plate 27 to prevent the aqueous solution from splashing, then close the door panel 23 to seal the outer box 21, and then start the central motor 31 to drive the second rotating shaft 32 to rotate, so that the belt 37 rotates to drive the rotating sleeve 36 on the support plate 35 to rotate, so that the long rod 38 rotates The solution in the splicing disk 27 is stirred to dissolve. When the splicing disk 27 is removed, the long rod 38 is lifted upward. After the splicing disk 27 is reset, the long rod 38 is moved downward and immersed in the solution. While the second rotating shaft 32 rotates, the screw ball 39 is driven to rotate, so that the screw ball 39 hits the knocking ball 310, causing the outer box 21 to vibrate, and then the splicing disk 27 to vibrate, further improving the stirring efficiency of the solution. When vibration is not required, the screw ball 39 is rotated to recycle it and no longer contact the knocking ball 310. After the chitosan is dissolved, different enzyme preparations are added to the splicing disk 27, and the reaction solution is enzymolyzed for a certain period of time, and the enzymolysis conditions in different splicing disks 27 are observed. Thereby, multiple portions of chitosan can be stirred and dissolved at the same time, so as to compare the enzymolysis effects of different enzyme preparations and select the best one. After the enzymolysis is completed, the reaction solution is poured out and filtered to obtain chitosan oligosaccharide.

[0036] like Figure 1 , Figure 7 and Figure 8 As shown, a locking mechanism 4 is also included, and the locking mechanism 4 includes an elastic locking plate 41, a retracting buckle 42, a synchronous plate 43, a back pressure plate 44, a guide rail 45 and a rotating plate 46. Five elastic locking plates 41 are connected to the inner side of the rear portion of the outer box 21, and five retracting buckles 42 are slidably connected to the rear side of the outer box 21. The retracting buckles 42 are all in contact with adjacent elastic locking plates 41, and a synchronous plate 43 is connected between the rear portions of the retracting buckles 42. A back pressure plate 44 is connected to the synchronous plate 43. Front and rear guide rails 45 are connected to the left and right sides of the middle portion of the outer box 21, and the guide rails 45 are slidably connected to the back pressure plate 44. A rotating plate 46 is connected to the middle portion of the first rotating shaft 22, and the rotating plate 46 is squeezed and matched with the back pressure plate 44.

[0037] By using the locking mechanism 4 of the device, the middle frame 26 can be locked. When the door panel 23 is opened, the first rotating shaft 22 drives the rotating plate 46 to rotate and squeeze the back pressure plate 44, so that the back pressure plate 44 moves backward along the guide rail 45, so that the synchronous plate 43 and the retracting buckle 42 move backward. When the retracting buckle 42 moves backward, the elastic locking plate 41 is squeezed inward and deformed. After the middle frame 26 is pushed onto the layered plate 25 and the door panel 23 is closed, the rotating plate 46 is disengaged from the back pressure plate 44, so that the elastic locking plate 41 is restored to be engaged with the middle frame 26, thereby locking the middle frame 26 and preventing the middle frame 26 from falling.

[0038] like Figure 1 and Fig. 9 As shown, it also includes a lower locking mechanism 5, which includes a compression spring 51 and an inclined fork plate 52. Five inclined fork plates 52 are slidably connected to the rear sides of the left and right parts of the outer box 21. The inclined fork plates 52 are all connected to the outer box 21 with compression springs 51, and the long rods 38 are slidably matched with adjacent inclined fork plates 52.

[0039] By using the lower locking mechanism 5 of the device, the long rod 38 can be automatically controlled. When the door panel 23 is closed, the inclined fork plate 52 is squeezed to move backward, and the compression spring 51 is squeezed and contracted, so that the long rod 38 moves downward along the inclined fork plate 52 and is immersed in the solution. After the door panel 23 is opened, the compression spring 51 rebounds, so that the inclined fork plate 52 moves forward and resets, and then the long rod 38 moves upward along the inclined fork plate 52 and is lifted, thereby automatically controlling the long rod 38 and improving the convenience of operation.

[0040] like Figure 1 and Fig.10 As shown, a ventilation mechanism 6 is also included. The ventilation mechanism 6 includes an outer cylinder 61 and a fan 62. The upper sides of the left and right parts of the outer box 21 are connected to the outer cylinder 61, and the fan 62 is installed on the outer cylinder 61.

[0041] By using the ventilation mechanism 6 of the device, the outer box 21 can be ventilated. When the interior of the outer box 21 needs to be ventilated, the fan 62 on the outer cylinder 61 is started, thereby ventilating the outer box 21, reducing the humidity inside the outer box 21, and preventing mold growth.

[0042] like Figure 1 and Fig.10 As shown, a temperature control mechanism 7 is also included. The temperature control mechanism 7 includes a temperature controller 71 and a temperature control tube 72. The temperature controller 71 is connected to both sides of the upper left and right sides of the outer box 21. The temperature controller 71 is provided with a display screen to display the temperature. Four temperature control tubes 72 are connected to the temperature controller 71, and the temperature control tubes 72 pass through the outer box 21.

[0043] By using the temperature control mechanism 7 of the device, the temperature inside the outer box 21 can be adjusted. When chitosan is enzymatically hydrolyzed in the splicing tray 27, the temperature controller 71 is started according to the enzymatic hydrolysis requirements, so that the temperature control tube 72 adjusts the temperature inside the outer box 21, thereby enabling chitosan to be enzymatically hydrolyzed at a suitable temperature and ensuring the enzymatic hydrolysis effect.

[0044] It should be understood that this embodiment is only used to illustrate the present invention and is not used to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope limited by the appended claims of the application.

Claims

1. A storage device for chitosan oligosaccharide cultivation, characterized in that: The invention comprises a base (1), a storage mechanism (2) and a slow stirring mechanism (3); the base (1) is provided with the storage mechanism (2) capable of storing the reaction liquid; the base (1) is also provided with the slow stirring mechanism (3) capable of stirring the reaction liquid.

2. A storage device for chitosan oligosaccharide cultivation as claimed in claim 1, characterized in that: The storage mechanism (2) comprises an outer box (21), a first rotating shaft (22), a door panel (23), a bracket (24), a layering tray (25), a middle frame (26) and a splicing tray (27). The upper side of the base (1) is connected to the outer box (21), the left and right sides of the front of the outer box (21) are both rotatably connected to the first rotating shaft (22), the first rotating shaft (22) is connected to the door panel (23), the upper side of the middle of the base (1) is connected to the bracket (24), a plurality of layering trays (25) are connected to the bracket (24), the layering trays (25) are all clamped with the middle frame (26), and the left and right sides of the middle frame (26) are both connected to the splicing tray (27).

3. A storage device for chitosan oligosaccharide cultivation as claimed in claim 2, characterized in that: The door panels (23) are all provided with handles.

4. A storage device for chitosan oligosaccharide cultivation as claimed in claim 2, characterized in that: The slow stirring mechanism (3) comprises a central motor (31), a second rotating shaft (32), a connecting frame (33), a fixed cover (34), a support plate (35), a rotating sleeve (36), a belt (37), a long rod (38) and a knocking assembly. The central motor (31) is connected to the lower side of the middle part of the base (1). The output shaft of the central motor (31) is connected to the second rotating shaft (32). The second rotating shaft (32) passes through the bracket (24) and is rotatably connected to the outer box (21). The upper part of the stratified disk (25) is connected to the connecting frame (33). The connecting frame (33) is connected to the fixed cover (34). The fixed cover (34) is connected to the adjacent stratified disk (25). The left and right sides of the bracket (24) are connected to a plurality of support plates (35). ), a rotating sleeve (36) is rotatably connected to the support plate (35), a belt (37) is wound around two laterally adjacent rotating sleeves (36), the belt (37) is in contact with the second rotating shaft (32), the diameter of the second rotating shaft (32) is larger than that of the rotating sleeve (36), a long rod (38) is slidably connected to the rotating sleeve (36), the long rod (38) passes through the adjacent fixed cover (34), a knocking component is provided between the second rotating shaft (32) and the outer box (21), the central motor (31) is started, the second rotating shaft (32) is driven to rotate, the belt (37) is rotated to drive the rotating sleeve (36) on the support plate (35) to rotate, and the long rod (38) is rotated to stir the solution so that the chitosan is dissolved.

5. A storage device for chitosan oligosaccharide cultivation as claimed in claim 4, characterized in that: The knocking assembly comprises a screw ball (39) and a knocking ball (310); a plurality of screw balls (39) are threadedly connected to the upper portion of the second rotating shaft (32); a plurality of knocking balls (310) are connected to the inner side of the upper portion of the outer box (21); when the second rotating shaft (32) rotates, the screw ball (39) is driven to rotate, so that the screw ball (39) hits the knocking ball (310), causing the outer box (21) to vibrate, thereby causing the splicing plate (27) to vibrate, thereby further improving the solution stirring efficiency.

6. A storage device for chitosan oligosaccharide cultivation as claimed in claim 5, characterized in that: The outer box (21) further comprises a locking mechanism (4), wherein the locking mechanism (4) comprises an elastic locking plate (41), a retracting buckle (42), a synchronous plate (43), a back pressure plate (44), a guide rail (45) and a rotating plate (46); a plurality of elastic locking plates (41) are connected to the inner side of the rear portion of the outer box (21); a plurality of retracting buckles (42) are slidably connected to the rear side of the outer box (21); the retracting buckles (42) are all in contact with adjacent elastic locking plates (41); a synchronous plate (43) is connected between the rear portions of the retracting buckles (42); a back pressure plate (44) is connected to the synchronous plate (43); two front and rear guide rails (45) are connected to the left and right sides of the middle portion of the outer box (21); the guide rails (45) are slidably connected to the back pressure plate (44); The first rotating shaft (22) is connected to a rotating plate (46) at the middle part thereof. The rotating plate (46) is pressed and matched with the return pressure plate (44). When the door panel (23) is opened, the first rotating shaft (22) drives the rotating plate (46) to rotate and press the return pressure plate (44), so that the return pressure plate (44) moves backward along the guide rail (45), so that the synchronous plate (43) and the retracting buckle (42) move backward. When the retracting buckle (42) moves backward, the elastic locking plate (41) is pressed and retracted inwardly to deform. After the middle frame (26) is pushed onto the layered plate (25) and the door panel (23) is closed, the rotating plate (46) is separated from the return pressure plate (44), so that the elastic locking plate (41) is restored to be engaged with the middle frame (26).

7. A storage device for chitosan oligosaccharide cultivation as claimed in claim 6, characterized in that: The invention also comprises a lower locking mechanism (5), which comprises a compression spring (51) and an inclined fork plate (52). The rear sides of the left and right parts of the outer box (21) are both slidably connected with a plurality of inclined fork plates (52). The inclined fork plates (52) are all connected to the outer box (21) with compression springs (51). The long rods (38) are all slidably matched with adjacent inclined fork plates (52). When the door panel (23) is closed, the inclined fork plates (52) are squeezed to move backwards, and the compression springs (51) are squeezed and contracted, so that the long rods (38) move downwards along the inclined fork plates (52) and are immersed in the solution. After the door panel (23) is opened, the compression springs (51) rebound, so that the inclined fork plates (52) move forward and reset, thereby causing the long rods (38) to move upwards along the inclined fork plates (52) and be lifted.

8. A storage device for chitosan oligosaccharide cultivation as claimed in claim 7, characterized in that: The device further comprises a ventilation mechanism (6), the ventilation mechanism (6) comprising an outer cylinder (61) and a fan (62), the upper sides of the left and right parts of the outer box (21) are both connected to the outer cylinder (61), and the fan (62) is installed on the outer cylinder (61). When the interior of the outer box (21) needs to be ventilated, the fan (62) on the outer cylinder (61) is started.

9. A storage device for chitosan oligosaccharide cultivation as claimed in claim 8, characterized in that: The outer box (21) further comprises a temperature control mechanism (7), wherein the temperature control mechanism (7) comprises a temperature controller (71) and a temperature control tube (72). The temperature controller (71) is connected to both left and right sides of the upper part of the outer box (21), and a plurality of temperature control tubes (72) are connected to the temperature controller (71). The temperature control tubes (72) all pass through the outer box (21). When chitosan is enzymatically hydrolyzed in the splicing plate (27), the temperature controller (71) is started according to the enzymatic hydrolysis requirements, so that the temperature control tubes (72) adjust the temperature inside the outer box (21).

10. A storage device for chitosan oligosaccharide cultivation as claimed in claim 9, characterized in that: The temperature controller (71) is provided with a display screen.

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