Cell incubator convenient for adding nutrient solution

Abstract

The invention relates to the technical field of culture devices, in particular to a cell culture box convenient for adding a nutrient solution, the cell culture box comprises a culture box, a downward moving stirring mechanism, a downward moving vibration mechanism and a heating pick-and-place mechanism are arranged in the culture box, and a sealing mechanism is arranged on the outer wall of the culture box; the downward-moving stirring mechanism comprises an electric push rod fixedly penetrating through the top end of the incubator, a hollow column is fixedly connected to the telescopic end of the electric push rod, a stirring shaft rotationally penetrates through the bottom end of the hollow column, and a plurality of stirring blades used for stirring the virulent phage colonies are fixedly connected to the outer wall of the stirring shaft. The electric push rod drives the stirring blades and the stirring shaft to move up and down through the hollow column; according to the invention, vibration treatment can be carried out on the strong bacteriophage in the stirring process through the downward-moving stirring mechanism and the downward-moving vibration mechanism, so that full contact of the strong bacteriophage and host cells is ensured, oxygen and nutrient substances in a culture medium are uniformly distributed, and a favorable culture environment is ensured.

Description

Technical Field

[0001] The invention relates to the technical field of culture devices, and in particular to a cell culture box which is convenient for adding nutrient solution. Background Art

[0002] Virulent phages adsorb to the surface of host bacteria through their tail fibers, inject their genomes into cells, replicate and reproduce using the metabolic system of the host bacteria, produce a large number of progeny phages, and ultimately lyse the host bacteria, releasing the progeny phages and intracellular substances. Virulent phage colonies need to be cultured and processed using a culture device during the culture process, which makes it convenient to maintain them during the culture process.

[0003] At present, in the prior art, when culturing virulent phages, they are stirred by a stirring mechanism. However, stirring alone will cause aggregation of virulent phages to form cell clusters or cell clusters. These cell clusters may hinder the uniform distribution of nutrients and the penetration of growth factors, leading to malnutrition and metabolic abnormalities inside the virulent phages. In addition, after stirring, the stirring blades will be placed in the culture dish for a long time, resulting in prolonged contact time with the virulent phages, increasing the influence of the external environment, and causing the virulent phages to be unable to be in a suitable growth environment, which is not conducive to the normal growth and reproduction of the virulent phages, and it is inconvenient for the experimenter to observe the growth and morphological changes of the virulent phages. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings of the background technology and to provide a cell culture box that is convenient for adding nutrient solution.

[0005] To achieve the above purpose, the technical solution adopted by the present invention is: a cell culture box that is convenient for adding nutrient solution, comprising a culture box, wherein a downward stirring mechanism, a downward vibration mechanism and a heating and placing mechanism are arranged inside the culture box, and a sealing mechanism is arranged on the outer wall of the culture box;

[0006] The downward stirring mechanism includes an electric push rod fixedly passing through the top of the incubator, the telescopic end of the electric push rod is fixedly connected to a hollow column, the bottom end of the hollow column is rotatably penetrated by a stirring shaft, the outer wall of the stirring shaft is fixedly connected to a plurality of stirring blades for stirring the virulent phage colony, the electric push rod moves up and down with the stirring blades and the stirring shaft through the hollow column, and leaves the virulent phage colony range after stirring;

[0007] The downward vibration mechanism includes limit rods symmetrically fixedly connected to the outer wall of the hollow column, the two limit rods are slidably connected to the inner wall of the incubator, one end of the two limit rods is fixedly connected to an elastic paddle, the bottom of the inner wall of the incubator is fixedly connected to a temperature control box through a circular column, the outer wall of the temperature control box is symmetrically fixedly connected to vertical rods, one end of the two vertical rods is fixedly connected to a plurality of metal plates, the limit rods move downward with the elastic paddles and paddle the metal plates to generate vibration.

[0008] Preferably, the downward stirring mechanism also includes an extension rod fixedly connected to the outer wall of the hollow column, a transmission shaft rotatably passes through the extension rod, one end of the transmission shaft is fixedly connected to a circular gear, the outer diameter of the circular gear is meshed with a toothed plate, and the toothed plate is fixedly connected to the incubator.

[0009] Preferably, the downward stirring mechanism also includes a first bevel gear fixedly connected to the top end of the stirring shaft, the outer diameter of the first bevel gear is meshingly connected to the second bevel gear, one end of the second bevel gear is fixedly connected to a rotating shaft, the rotating shaft and the hollow column are rotatably connected, the outer wall of the rotating shaft is fixedly connected to a second pulley, the other end of the transmission shaft is fixedly connected to the first pulley, the first pulley and the second pulley are connected by a belt, and a hole is penetrated on one side of the outer wall of the hollow column corresponding to the extension rod, and the hole is arranged on the outside of the belt.

[0010] Preferably, the two upright poles are fixedly connected to a connecting rod at one end away from each other, and the two connecting rods are fixedly connected to the incubator.

[0011] Preferably, the heating and placing mechanism includes an annular placement groove opened at the top of the temperature control box, a limiting ring is provided on the inner wall of the annular placement groove, a handle is symmetrically fixedly connected to the top of the limiting ring, a culture dish is fixedly connected to the inner wall of the limiting ring, and a distance is left between the culture dish and the temperature control box.

[0012] Preferably, clamping columns are slidably penetrated at both ends of the temperature control box and extend into the annular placement groove, and clamping grooves matching the shape of the clamping columns are formed at both ends of the limiting ring.

[0013] Preferably, the ends of the two clamping columns away from the temperature control box are fixedly connected to vertical blocks, the ends of the two vertical blocks close to the temperature control box are fixedly connected to tension springs at the lower sides of the corresponding clamping columns, and the two tension springs are fixedly connected to the temperature control box.

[0014] Preferably, the heating and placing mechanism further comprises a water inlet pipe and a water outlet pipe fixedly passing through the two ends of the temperature control box and passing through the incubator, and a temperature sensor is fixedly passed through the temperature control box.

[0015] Preferably, the sealing mechanism includes an observation window penetrated through the incubator, a storage groove is provided at the top of the observation window, a transparent plate is provided on the inner wall of the storage groove, a fixed block is fixedly connected to the outer wall of the transparent plate, and an electric telescopic rod is fixedly connected between the fixed block and the incubator.

[0016] Preferably, a sealing groove matching the shape of the transparent plate is provided at the bottom of the observation window, and two rubber sealing rods are fixedly connected to both ends of the observation window and are arranged on both sides of the transparent plate.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. By setting the downward stirring mechanism, the stirring blade can be rotated during the downward movement, so that the virulent phage can be evenly distributed in the culture dish, so that the virulent phage can fully contact with the host cell, improve the infection efficiency and proliferation rate of the virulent phage, and change the uniformity of the fermentation liquid. After stirring, the stirring blade can be moved away from the range of the virulent phage, reducing the contact time with the virulent phage, so that the virulent phage colony can be in a more suitable growth environment, which is conducive to its normal growth and reproduction, and also allows the experimenter to more clearly observe the growth and morphological changes of the virulent phage colony, which is convenient for maintenance;

[0019] 2. The setting of the downward vibration mechanism can help improve the distribution of oxygen and nutrients in the culture medium during the stirring process, provide a more favorable environment for the growth of virulent phages, and also help the phages to be more evenly distributed in the culture medium, thereby increasing the chances of virulent phages contacting host cells;

[0020] 3. By setting up the heating and placing mechanism, the culture dish can be prevented from being disengaged and tilted during the vibration process, and the virulent phage colonies in the culture dish can be prevented from falling off, thereby ensuring stability during the vibration process. In addition, the culture dish can be heated during the culture process, and the hot water can wrap the bottom and sides of the entire culture dish, so that the virulent phage colonies in the culture dish can be evenly heated to ensure the heating effect. At the end of the culture, the cold water can be quickly replaced to prevent the growth of virulent phages.

[0021] 4. The sealing mechanism can prevent the entry of external microorganisms during the culture process, thereby avoiding contamination of the virulent phage colonies. The growth of the virulent phage colonies can be observed through the transparent plate, which facilitates maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is an overall schematic diagram of a cell culture box that is convenient for adding nutrient solution according to the present invention;

[0023] Figure 2 A cross-sectional view of a cell culture box that is convenient for adding nutrient solution according to the present invention;

[0024] Figure 3 It is a partial structural schematic diagram of a cell culture box for adding nutrient solution according to the present invention;

[0025] Figure 4 A cross-sectional view of a hollow column of a cell culture box for convenient addition of nutrient solution according to the present invention;

[0026] Figure 5 A cross-sectional view of a temperature control box of a cell culture box for adding nutrient solution according to the present invention;

[0027] Figure 6 This is a diagram showing the separation of a temperature control box and a culture dish of a cell culture box that is convenient for adding nutrient solution according to the present invention;

[0028] Figure 7 A cell culture box for adding nutrient solution conveniently provided by the present invention Figure 6 Enlarged view of point A in the middle;

[0029] Figure 8 A sectional top view of a transparent plate of a cell culture box for adding nutrient solution according to the present invention;

[0030] Fig. 9 The present invention is a cross-sectional bottom view of a transparent plate of a cell culture box for convenient addition of nutrient solution.

[0031] In the figure: 1. incubator; 2. electric push rod; 3. electric telescopic rod; 4. transparent plate; 5. fixed block; 6. water inlet pipe; 7. hollow column; 8. limit rod; 9. metal plate; 10. vertical pole; 11. water outlet pipe; 12. culture dish; 13. temperature sensor; 14. temperature control box; 15. stirring blade; 16. stirring shaft; 17. tooth plate; 18. extension rod; 19. circular gear; 20. transmission shaft; 21. first pulley; 22. hole; 23. limit ring; 24. connecting rod; 25. handle; 26. elastic paddle; 27. first bevel gear; 28. second bevel gear; 29. ​​rotating shaft; 30. second pulley; 31. slot; 32. annular placement slot; 33. clamping column; 34. vertical block; 35. tension spring; 36. observation window; 37. sealing slot; 38. rubber sealing rod; 39. storage slot. DETAILED DESCRIPTION

[0032] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are only examples, and those skilled in the art may think of other obvious variations.

[0033] like Figure 1-Figure 9A cell culture box for adding nutrient solution is shown, comprising a culture box 1, wherein a downward stirring mechanism, a downward vibration mechanism and a heating and placing mechanism are arranged inside the culture box 1, and a sealing mechanism is arranged on the outer wall of the culture box 1. The arrangement of the downward stirring mechanism can stir the virulent bacteriophage during the downward movement, so that the virulent bacteriophage is evenly distributed in the culture dish 12, and the stirring blade 15 is moved away from the range of the virulent bacteriophage after the culture is completed, the downward vibration mechanism can assist the downward stirring mechanism, the heating and placing mechanism can limit the culture dish 12, and the virulent bacteriophage can be evenly and quickly heated, and the sealing mechanism can provide a sterile environment during the culture process;

[0034] like Figure 2 , Figure 4 As shown, the downward stirring mechanism includes an electric push rod 2 fixedly passing through the top of the incubator 1, the telescopic end of the electric push rod 2 is fixedly connected to a hollow column 7, the bottom end of the hollow column 7 is rotatably penetrated by a stirring shaft 16, and the outer wall of the stirring shaft 16 is fixedly connected to a plurality of stirring blades 15 for stirring the virulent phage colony, the electric push rod 2 moves up and down with the stirring blades 15 and the stirring shaft 16 through the hollow column 7, and leaves the virulent phage colony range after stirring;

[0035] like Figure 2 , Figure 3 As shown, the downward vibration mechanism includes limit rods 8 symmetrically fixedly connected to the outer wall of the hollow column 7, the two limit rods 8 are slidably connected to the inner wall of the incubator 1, one end of the two limit rods 8 is fixedly connected with an elastic paddle 26, the bottom of the inner wall of the incubator 1 is fixedly connected to the temperature control box 14 through a circular column, the outer wall of the temperature control box 14 is symmetrically fixedly connected with vertical rods 10, one end of the two vertical rods 10 is fixedly connected with a plurality of metal plates 9, the limit rods 8 move downward with the elastic paddle 26 and paddle the metal plate 9 to generate vibration.

[0036] like Figure 3 , Figure 4 As shown, the downward stirring mechanism also includes an extension rod 18 fixedly connected to the outer wall of the hollow column 7, a transmission shaft 20 is rotatably penetrated on the extension rod 18, one end of the transmission shaft 20 is fixedly connected to a circular gear 19, the outer diameter of the circular gear 19 is meshedly connected to a toothed plate 17, and the toothed plate 17 is fixedly connected to the incubator 1. The transmission shaft 20 is limited by the extension rod 18, and when the circular gear 19 moves downward, it rotates along the outer diameter of the toothed plate 17.

[0037] like Figure 3 , Figure 4As shown, the downward stirring mechanism also includes a first bevel gear 27 fixedly connected to the top of the stirring shaft 16, the outer diameter of the first bevel gear 27 is meshed with a second bevel gear 28, one end of the second bevel gear 28 is fixedly connected to a rotating shaft 29, the rotating shaft 29 is rotatably connected to the hollow column 7, the outer wall of the rotating shaft 29 is fixedly connected to a second pulley 30, the other end of the transmission shaft 20 is fixedly connected to a first pulley 21, the first pulley 21 and the second pulley 30 are connected by a belt, and a hole 22 is pierced on one side of the outer wall of the hollow column 7 corresponding to the extension rod 18, and the hole 22 is set on the outside of the belt. When the circular gear 19 rotates, it will drive the transmission shaft 20 to rotate, thereby driving the first pulley 21, and the first pulley 21 will drive the second pulley 30 and the rotating shaft 29 to rotate through the belt, and then the stirring shaft 16 and the stirring blade 15 will rotate through the first bevel gear 27 and the second bevel gear 28 to complete the stirring of the virulent bacteriophage.

[0038] like Figure 3 As shown, the ends of the two vertical rods 10 away from each other are fixedly connected with connecting rods 24, and the two connecting rods 24 are fixedly connected to the incubator 1. The vertical rods 10 are limited and fixed by the connecting rods 24 to prevent the vertical rods 10 from breaking during the vibration process.

[0039] like Figure 5-Figure 7 As shown, the heating and placing mechanism includes an annular placement groove 32 opened at the top of the temperature control box 14, a limiting ring 23 is arranged on the inner wall of the annular placement groove 32, a handle 25 is symmetrically fixedly connected to the top of the limiting ring 23, and a culture dish 12 is fixedly connected to the inner wall of the limiting ring 23, and a distance is left between the culture dish 12 and the temperature control box 14. The limiting ring 23 is higher than the annular placement groove 32, and the limiting ring 23 can be lifted up by the handle 25, so that the culture dish 12 can be lifted up, which is convenient for taking and placing the culture dish 12, and the distance between the culture dish 12 and the temperature control box 14 can make water of different temperatures wrap the entire culture dish 12, so that it can be quickly and evenly heated, and cold water can also be quickly switched to prevent the growth of virulent bacteriophage colonies.

[0040] like Figure 6 , Figure 7 As shown, the two ends of the temperature control box 14 are slidably penetrated by a clamping column 33 and extend into the annular placement groove 32, and the two ends of the limit ring 23 are provided with a clamping groove 31 that matches the shape of the clamping column 33, and the ends of the two clamping columns 33 away from the temperature control box 14 are fixedly connected to a vertical block 34, and the ends of the two vertical blocks 34 close to the temperature control box 14 are fixedly connected to the lower side of the clamping column 33, and the two tension springs 35 are fixedly connected to the temperature control box 14. When the clamping column 33 is inserted into the clamping groove 31, the limit ring 23 can be fixed, and the clamping column 33 can always be inserted into the clamping groove 31 through the reset effect of the tension spring 35 and the cooperation of the vertical block 34.

[0041] like Figure 2 , Figure 5 As shown, the heating and placing mechanism also includes a water inlet pipe 6 and a water outlet pipe 11 fixedly passing through the two ends of the temperature control box 14 and passing through the incubator 1. A temperature sensor 13 is fixedly passed through the temperature control box 14. The temperature sensor 13 is a prior art and will not be described here. Water of appropriate temperature is injected into the temperature control box 14 through the water inlet pipe 6, and the temperature in the temperature control box 14 is monitored by the temperature sensor 13, and the used water is discharged through the water outlet pipe 11, thereby promoting the flow of water.

[0042] like Figure 8 , Fig. 9 As shown, the sealing mechanism includes an observation window 36 penetrated on the incubator 1, a storage slot 39 is provided at the top of the observation window 36, a transparent plate 4 is provided on the inner wall of the storage slot 39, a fixing block 5 is fixedly connected to the outer wall of the transparent plate 4, and an electric telescopic rod 3 is fixedly connected between the fixing block 5 and the incubator 1. The cultivation of the virulent bacteriophage colony can be observed through the transparent plate 4, so as to facilitate the processing and maintenance thereof, and the electric telescopic rod 3 can be moved with the fixing block 5, so that the transparent plate 4 can be stored in the storage slot 39, so as to facilitate the removal of the culture dish 12 after the cultivation is completed, and the arrangement here also facilitates the addition and replacement of the nutrient solution, bringing convenience.

[0043] like Figure 8 , Fig. 9 As shown, a sealing groove 37 matching the shape of the transparent plate 4 is provided at the bottom of the observation window 36, and two rubber sealing rods 38 are fixedly connected at both ends of the observation window 36 and arranged on both sides of the transparent plate 4. The sealing groove 37 and the rubber sealing rod 38 can achieve a sealing effect to prevent external microorganisms from entering during the culture process, thereby avoiding affecting the culture of virulent phage colonies.

[0044] Working principle: first, the electric telescopic rod 3 is started to move the fixed block 5 and the transparent plate 4 upward and open the observation window 36, then the vertical block 34 is pulled outward and the clamping column 33 is moved to leave the range of the annular placement groove 32, and the limiting ring 23 is placed in the annular placement groove 32, then the vertical block 34 is released and the clamping column 33 is clamped into the clamping groove 31 under the elastic reset action of the tension spring 35 to complete the limiting of the limiting ring 23, thereby completing the limiting of the culture dish 12, and then the electric telescopic rod 3 is reversely started to insert the transparent plate 4 into the sealing groove 37 and complete the sealing operation with the cooperation of the rubber sealing rod 38, so as to prevent the entry of external microorganisms during the culture process and avoid affecting the culture operation of the virulent bacteriophage;

[0045] Further, the electric push rod 2 is started to move the hollow column 7 downward, and the circular gear 19 moves downward along one end of the tooth plate 17 and rotates itself, thereby rotating the transmission shaft 20, and the transmission shaft 20 rotates with the first pulley 21, and the first pulley 21 rotates with the second pulley 30 and the rotating shaft 29 through the belt, thereby rotating the second bevel gear 28 and the first bevel gear 27, and the first bevel gear 27 rotates with the stirring shaft 16 and the stirring blade 15. When the stirring blade 15 enters the range of the culture dish 12, it will stir the virulent phage in the culture dish 12, so that the virulent phage and the host cells are fully in contact, thereby improving the infection efficiency and proliferation rate of the virulent phage, and changing the uniformity of the fermentation liquid. , and in the process of moving downward, the limit rod 8 and the elastic paddle 26 will move downward, and the metal plate 9 will be moved to produce a vibration effect. Since the metal plate 9 is fixed on the vertical rod 10, the vibration will be transmitted to the temperature control box 14, which can help improve the distribution of oxygen and nutrients in the culture medium during the stirring process, and provide a more favorable environment for the growth of virulent phages. When the stirring is completed, the electric push rod 2 is reversely started to move the hollow column 7 upward until the stirring blade 15 leaves the range of the culture dish 12, thereby reducing the contact time with the virulent phage, preventing the microorganisms on the stirring blade 15 from contaminating the virulent phage colony, and allowing the virulent phage colony to be in a more suitable growth environment, which is conducive to its normal growth and reproduction;

[0046] Furthermore, water of suitable temperature is injected into the temperature control box 14 through the water inlet pipe 6, and the water temperature is monitored by the temperature sensor 13 to reach a suitable temperature. The water will fully surround the bottom and sides of the culture dish 12, so that it can quickly reach a suitable temperature and culture the virulent phage colonies to ensure the heating effect.

[0047] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions only describe the principles of the present invention. The present invention may be subject to various changes and improvements without departing from the spirit and scope of the present invention. These changes and improvements fall within the scope of the present invention. The scope of protection claimed by the present invention is defined by the attached claims and their equivalents.

Claims

1. A cell culture incubator that facilitates the addition of nutrient solution, comprising an incubator (1), characterized in that: The incubator (1) is provided with a downward stirring mechanism, a downward vibration mechanism and a heating and placing mechanism inside, and a sealing mechanism is provided on the outer wall of the incubator (1); The downward moving stirring mechanism comprises an electric push rod (2) fixedly passing through the top of the incubator (1); the telescopic end of the electric push rod (2) is fixedly connected to a hollow column (7); a stirring shaft (16) is rotatably passed through the bottom end of the hollow column (7); a plurality of stirring blades (15) for stirring the virulent phage colony are fixedly connected to the outer wall of the stirring shaft (16); the electric push rod (2) moves up and down with the stirring blades (15) and the stirring shaft (16) through the hollow column (7), and leaves the virulent phage colony range after stirring; The downward vibration mechanism comprises a limit rod (8) symmetrically fixedly connected to the outer wall of the hollow column (7); the two limit rods (8) are both slidably connected to the inner wall of the incubator (1); one end of the two limit rods (8) is fixedly connected to an elastic paddle (26); the bottom of the inner wall of the incubator (1) is fixedly connected to a temperature control box (14) via a circular column; the outer wall of the temperature control box (14) is symmetrically fixedly connected to a vertical rod (10); one end of the two vertical rods (10) is fixedly connected to a plurality of metal plates (9); the limit rod (8) moves downward with the elastic paddle (26) and paddles the metal plates (9) to generate vibration.

2. A cell culture incubator that facilitates the addition of nutrient solution according to claim 1, characterized in that: The downward stirring mechanism also includes an extension rod (18) fixedly connected to the outer wall of the hollow column (7), a transmission shaft (20) rotatably passing through the extension rod (18), one end of the transmission shaft (20) is fixedly connected to a circular gear (19), the outer diameter of the circular gear (19) is meshingly connected to a toothed plate (17), and the toothed plate (17) is fixedly connected to the incubator (1).

3. A cell culture incubator that facilitates the addition of nutrient solution according to claim 2, characterized in that: The downward moving stirring mechanism also includes a first bevel gear (27) fixedly connected to the top end of the stirring shaft (16); the first bevel gear (27) is meshingly connected to the outer diameter of the first bevel gear (27); one end of the second bevel gear (28) is fixedly connected to a rotating shaft (29); the rotating shaft (29) and the hollow column (7) are rotatably connected; the outer wall of the rotating shaft (29) is fixedly connected to a second pulley (30); the other end of the transmission shaft (20) is fixedly connected to a first pulley (21); the first pulley (21) and the second pulley (30) are connected via a belt; a hole (22) is provided on the outer wall of the hollow column (7) corresponding to one side of the extension rod (18); the hole (22) is arranged on the outer side of the belt.

4. The cell culture incubator for adding nutrient solution according to claim 1, characterized in that: The two upright poles (10) are fixedly connected to a connecting rod (24) at one end away from each other, and the two connecting rods (24) are fixedly connected to the incubator (1).

5. The cell culture box for adding nutrient solution according to claim 1, characterized in that: The heating and placing mechanism comprises an annular placement groove (32) provided at the top of the temperature control box (14); a limiting ring (23) is arranged on the inner wall of the annular placement groove (32); a handle (25) is symmetrically fixedly connected to the top of the limiting ring (23); a culture dish (12) is fixedly connected to the inner wall of the limiting ring (23); and a distance is left between the culture dish (12) and the temperature control box (14).

6. A cell culture incubator for adding nutrient solution according to claim 5, characterized in that: The two ends of the temperature control box (14) are slidably penetrated by clamping columns (33) and extend into the annular placement groove (32), and the two ends of the limit ring (23) are provided with clamping grooves (31) that are matched with the shape of the clamping columns (33).

7. A cell culture incubator for adding nutrient solution according to claim 6, characterized in that: The ends of the two clamping columns (33) away from the temperature control box (14) are fixedly connected to a vertical block (34), and the ends of the two vertical blocks (34) close to the temperature control box (14) are fixedly connected to the lower sides of the corresponding clamping columns (33), and the two tension springs (35) are fixedly connected to the temperature control box (14).

8. The cell culture box for adding nutrient solution according to claim 5, characterized in that: The heating and placing mechanism also includes a water inlet pipe (6) and a water outlet pipe (11) which are fixedly passed through the two ends of the temperature control box (14) and pass through the incubator (1); a temperature sensor (13) is fixedly passed through the temperature control box (14).

9. The cell culture incubator for adding nutrient solution according to claim 1, characterized in that: The sealing mechanism comprises an observation window (36) penetrated through the incubator (1), a storage groove (39) is provided at the top of the observation window (36), a transparent plate (4) is provided on the inner wall of the storage groove (39), a fixed block (5) is fixedly connected to the outer wall of the transparent plate (4), and an electric telescopic rod (3) is fixedly connected between the fixed block (5) and the incubator (1).

10. The cell culture box for adding nutrient solution according to claim 9, characterized in that: The bottom end of the observation window (36) is provided with a sealing groove (37) matching the shape of the transparent plate (4), and two rubber sealing rods (38) are fixedly connected to both ends of the observation window (36) and are arranged on both sides of the transparent plate (4).

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