Hypsizygus marmoreus strain suitable for white beech mushroom production

Abstract

The invention discloses a hypsizygus marmoreus strain suitable for white beech mushroom production. In the invention, the white beech mushroom strain white beech mushroom 87 is formed by hybridizing and breeding hypsizigus marmoreus' B02 'and hypsizigus marmoreus' B06 'as parents. The white beech mushroom strain 'B02' has the characteristics of uniform stipe thickness, high yield and the like, but the stipe is thin, yellow and the like, the 'B06' has the characteristics of thick mushroom caps, thicker stipe, white color, higher yield, but the defects of easy generation of deformed buds, poor uniformity and the like, and the white beech mushroom strain 'B07' is classified and named as Hypsizygus marmoreus 87, is preserved in China Center for Type Culture Collection on November 7, 2024, and has the preservation number of CGMCC NO. The preservation number is CCTCC M 20242461, and the preservation address is No. 299 on eight road in Wuchang District, Wuhan City, Hubei Province.

Description

Technical Field

[0001] The invention belongs to the technical field of white jade mushroom breeding, and specifically relates to a Pleurotus eryngii fungus species suitable for white jade mushroom production. Background Art

[0002] Hypsizygus marmoreus belongs to the phylum Basidiomycetes, class Hymeromycetes, order Agaricales, family Trichoderma, genus Hypsizygus. Its common names include enokitake and shimeji mushroom. It is one of the three major varieties currently cultivated in factories in my country. Hypsizygus marmoreus has different variations in the process of cultivation and breeding. According to the color of the fruiting bodies and the length of the stalks of Hypsizygus marmoreus sold on the market, its commercial names include oyster mushroom, white jade mushroom, seafood mushroom, etc. Among them, white jade mushroom refers to Hypsizygus marmoreus with white fruiting bodies and short stalks. Its cultivation method is different from that of oyster mushroom and seafood mushroom. Therefore, people carry out directional breeding of Hypsizygus marmoreus according to the different categories of Hypsizygus marmoreus sold on the market.

[0003] The cultivation technology of Agrocybe agaricus in my country was introduced from Japan. At present, most of the varieties used in factories in my country are cultivated in Japan, and a considerable amount of variety or technology royalties need to be paid to Japan every year. With the continuous exploration of Agrocybe agaricus cultivation technology by Chinese researchers and growers, a set of Agrocybe agaricus cultivation technology suitable for China's national conditions has gradually been formed. It is also found that the varieties introduced from Japan have more or less some defects in cultivation, especially with the increasingly competitive market in recent years, the commercial requirements for varieties are getting higher and higher. Summary of the invention

[0004] The object of the present invention is to provide a species of Agaricus spp. suitable for producing Agaricus spp. in order to solve the above-mentioned problems.

[0005] The technical solution adopted by the present invention is as follows: a Hypsizygus marmoreus strain suitable for the production of white jade mushroom, the white jade mushroom strain 'Bai Yu Mushroom 87' is classified and named Hypsizygus marmoreus Bai Yu Mushroom 87, which has been preserved in the China Center for Type Culture Collection on November 7, 2024, with a preservation number of CCTCC M20242461, and the preservation address is No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0006] In a preferred embodiment, the white jade mushroom strain (Hypsizygus marmoreus) white jade mushroom 87 is bred by hybridization with Hypsizygus marmoreus 'B02' and Hypsizygus marmoreus 'B06' as parents. 'B02' has the characteristics of uniform thickness of the stem and high yield, but has the disadvantages of thin and yellow stem; 'B06' has the characteristics of thick cap, thick stem, white color and high yield, but has the disadvantages of easy deformed buds and slightly poor uniformity. It is the main cultivated variety of white jade mushroom of Fujian Wanchen Biotechnology Group Co., Ltd. According to the cultivation test, the fruiting body of 'Bai Yu Mushroom 87' has good overall uniformity, white color, round and thick cap, good stem hardness, high yield, and inherits the advantages of the two parents.

[0007] In a preferred embodiment, the mycelium of 'Bai Yu Mushroom 87' on PDA plate culture medium is white and fluffy, and its mycelium can grow at 10-30°C, wherein the growth is slower and the mycelium is thinner at 10°C and 30°C; at 25°C, the colony grows well and the mycelium grows the thickest, densest and whitest.

[0008] In a preferred embodiment, the average single-bottle yield of 'Bai Yu Mushroom 87' is 336.5 g, which is 21.96% higher than the certified variety 'Nong Wan Zhen No. 1' (average single-bottle yield is 275.9 g) in Fujian Province. In addition, the cap diameter, thickness, stipe diameter and length of 'Bai Yu Mushroom 87' are all greater than those of 'Nong Wan Zhen No. 1'.

[0009] In a preferred embodiment, the use of a Pleurotus eryngii strain suitable for producing Pleurotus eryngii in Pleurotus eryngii breeding comprises the following steps:

[0010] 1) Creation of breeding materials: With 'B02' and 'B06' as breeding parents, after fruiting test, the spores of the two parents were ejected when their fruiting bodies matured, and spore germination was carried out. The germinated spores were picked up under a microscope and confirmed by lock-like union. The monospore strains without lock-like union were selected as breeding materials, and finally 44 monospore strains of 'B02' and 19 monospore strains of 'B06' were obtained.

[0011] (2) Hybridization pairing: 10 monospore strains of 'B02' and 'B06' with good growth were selected and paired in pairs, for a total of 100 combinations. After microscopic examination, 37 hybrids with lock-like unions were obtained, that is, 37 hybrids were obtained. Among them, 2 hybrid strains grew poorly in the mycelium stage and were discarded.

[0012] (3) Hybrid screening: The 35 hybrid strains with normal growth obtained by hybridization were screened for fruiting test. Among them, 6 hybrid strains showed good fruiting performance in the initial screening. These 6 were arranged for the re-screening experiment.

[0013] (4) The second fruiting screening of the 6 hybrids was performed. All of the 6 hybrids were able to produce mushrooms normally, but there were differences in the properties and fresh weight per bottle of each hybrid strain. Among them, the hybrid with the fruiting number B0087 had the best overall properties. Its fruiting body was uniform, the mushroom body was white, the mushroom stem was thick, the hardness was good, the mushroom cap was round, and the fresh weight per bottle was 345g, which was much higher than the parent 'B06' suitable for the cultivation of white jade mushroom (single bottle fresh weight reached 270g), and 6.28% higher than the other parent 'B02' 324.6g. Therefore, the hybrid white jade mushroom 87 inherited the advantages of high yield of the parent 'B02', and also inherited the characteristics of white fruiting body and thick stem of the parent 'B06'. The hybrid of the spotted jade mushroom B0087 was named white jade mushroom 87.

[0014] In a preferred embodiment, in step 1, the mother culture medium for creating the breeding material is PDA medium, the formula is: 200g potato, 20g glucose, 20g agar, 1000mL water

[0015] In a preferred embodiment, in step 1, the formula of the culture medium used for the original seed production and the substitute planting is: 47% corn cobs, 25% rice bran, 7% bran, 2% cottonseed hulls, 5% bean hulls, 4% active agent, 8% beet pulp, 2% husk ash, 70% water content, pH 6.1-6.5. Each bottle contains about 243 grams of dry material.

[0016] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0017] In the present invention, the strain is obtained by hybridization and breeding with Agrocybe 'B02' and Agrocybe 'B06' as parents. The fruiting body of 'Bai Yu Mushroom 87' has good overall uniformity, white color, round and thick mushroom cap, good hardness of mushroom stem, high yield, and good development and application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 These are the fruiting bodies of the Pleurotus eryngii strain 'Pleurotus eryngii 87' and its parents 'B02' and 'B06', among which the Pleurotus eryngii strain 'Pleurotus eryngii 87' is the middle picture, the parent B02 is the left picture, and the fruiting body of the parent B06 is the right picture.

[0019] Figure 2 This is a schematic diagram of the specific fragment amplification results of the Pleurotus eryngii strain 'Pleurotus eryngii 87' and its parents B02 and B06.

[0020] Figure 3 The PCR electrophoresis diagrams of the specific fragments of the Pleurotus eryngii strain 'Pleurotus eryngii 87' and its parents B02 and B06, where 1 is B02; 2 is Pleurotus eryngii 87; and 3 is B06.

[0021] Figure 4These are the fruiting bodies of the Pleurotus ostreatus strains 'Pleurotus ostreatus 87' and Nong Wanzhen No. 1. The one on the left is Pleurotus ostreatus 87, and the one on the right is Nong Wanzhen No. 1.

[0022] Figure 5 These are the fruiting bodies of the Pleurotus ostreatus strains 'Pleurotus ostreatus 87' and Nong Wanzhen No. 6. The fruiting body of Pleurotus ostreatus is on the right side of the picture, and the fruiting body of Nong Wanzhen No. 6 is on the right side of the picture. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0024] Reference Figure 1-5 ,

[0025] A species of Agaricus scaber suitable for the production of Agaricus scaber.

[0026] The tested Agaricus eryngii strains 'B02' and 'B06' were provided by Fujian Wanchen Biotechnology Co., Ltd., and 'Nong Wanzhen No. 1' was a variety bred by our team in cooperation with Fujian Wanchen Biotechnology Co., Ltd.

[0027] In the following examples, the mother culture medium is PDA medium, and the formula is: 200 g potato, 20 g glucose, 20 g agar, and 1000 mL water.

[0028] In the following examples, the culture material formula used for the original seed production and substitute planting is: 47% corn cobs, 25% rice bran, 7% bran, 2% cottonseed hulls, 5% bean hulls, 4% active agent, 8% beet pulp, 2% husk ash, 70% water content, pH 6.1-6.5. Each bottle contains about 243 grams of dry material.

[0029] Example 1: Breeding of Baiyu Mushroom 'Baiyu Mushroom 87':

[0030] The breeding of Baiyu Mushroom 'Baiyu Mushroom 87' includes the following steps:

[0031] (1) Creation of breeding materials: 'B02' and 'B06' were used as breeding parents. After fruiting tests, spores of the two parents were ejected when their fruiting bodies matured, and spore germination was carried out. The germinated spores were picked up under a microscope and confirmed by lock union. The monosporic strains without lock union were selected as breeding materials. Finally, 44 monosporic strains of 'B02' and 19 monosporic strains of 'B06' were obtained.

[0032] (2) Hybridization pairing: 10 monospore strains of 'B02' and 'B06' with good growth were selected and paired in pairs, for a total of 100 combinations. After microscopic examination, 37 hybrids with lock-like unions were obtained, that is, 37 hybrids were obtained. Among them, 2 hybrid strains grew poorly in the mycelium stage and were discarded.

[0033] (3) Hybrid screening: The 35 hybrid strains with normal growth obtained by hybridization were screened for fruiting test. Among them, 6 hybrid strains showed good fruiting performance in the initial screening. These 6 were arranged for the re-screening experiment.

[0034] (4) The second fruiting screening of the 6 hybrids was performed. All of the 6 hybrids were able to produce mushrooms normally, but there were differences in the properties and fresh weight per bottle of each hybrid strain. Among them, the hybrid with the fruiting number B0087 had the best overall properties. Its fruiting body was uniform, the mushroom body was white, the mushroom stem was thick, the hardness was good, the mushroom cap was round, and the fresh weight per bottle was 345g, which was much higher than the parent 'B06' suitable for the cultivation of white jade mushroom (single bottle fresh weight reached 270g), and 6.28% higher than the other parent 'B02' 324.6g. Therefore, the hybrid white jade mushroom 87 inherited the advantages of high yield of the parent 'B02', and also inherited the characteristics of white fruiting body and thick stem of the parent 'B06'. The hybrid of the spotted jade mushroom B0087 was named white jade mushroom 87.

[0035] The white jade mushroom strain 'Bai Yu Mushroom 87' was classified and named Hypsizygus marmoreus Bai Yu Mushroom 87, which was deposited in the China Center for Type Culture Collection on November 7, 2024, with the deposit number CCTCC M 20242461, and the deposit address is No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0036] According to the genome sequencing data, the whole genome sequencing data of the white jade mushroom strain of the present invention are compared with each other by using Kmer subtractive assembly technology to obtain specific difference fragment sequences (SVs), and 16 SV sequences (sequences are shown in SEQ ID NO.1-16) are selected to design specific primers (P1-P6), and the white jade mushroom 'white jade mushroom 87', the parent 'B02', and the parent 'B06' are amplified. The PCR amplification results of each specific fragment are as follows: Figure 3 and Figure 4 As shown: P1-P5 are common fragments of 'Bai Yu Mushroom 87' and 'B02', which are different from 'B06'; P6-P10 are common sequences of 'Bai Yu Mushroom 87' and 'B06', which are different from 'B02'; P11-P13 are unique sequences of 'B02' that are different from 'Bai Yu Mushroom 87' and 'B06'; P14-P16 are unique sequences of 'B06' that are different from 'Bai Yu Mushroom 87' and 'B02'.

[0037] Based on the above test results, it can be concluded that there are significant differences in the DNA fingerprints of Pleurotus eryngii 'Pleurotus eryngii 87', B02 and B06, and they are definitely different varieties.

[0038] Example 2: DNA fingerprints of Pleurotus eryngii 'Pleurotus eryngii 87' and its parents B02 and B06:

[0039] The DNA samples were sent to Beijing Novogene Bioinformatics Technology Co., Ltd. for whole genome sequencing. After the sequencing data were obtained, the Kmer subtractive assembly technology was used to compare the whole genome sequencing data of 'Baiyugu 87', the parent 'B02' and the parent 'B06' to obtain specific differential fragment sequences (SVs). These differential fragment sequences were then used to design specific primers, where the primer sequences are shown in Table 1; after the primers were synthesized, PCR verification was performed on the samples to be tested, and the PCR products were tested. The results of PCR amplification of each specific fragment are shown in Figures X and X: P1-P5 are common fragments of 'Bai Yu Mu 87' and 'B02', which are different from 'B06'; P6-P10 are common sequences of 'Bai Yu Mu 87' and 'B06', which are different from 'B02'; P11-P13 are unique sequences of 'B02' that are different from 'Bai Yu Mu 87' and 'B06'; P14-P16 are unique sequences of 'B06' that are different from 'Bai Yu Mu 87' and 'B02'. The results of specific molecular detection show that the hybrid 'Bai Yu Mu 87' is different from its parents 'B02' and 'B06' in genetics and is a different species.

[0040] Table 1: Primer sequences of SV

[0041]

[0042]

[0043] The DNA sequences of each specific fragment are:

[0044] P1(813bp)

[0045] TGAATGCGAAGGGGGTTTGATTTGTTGGCCACAGATGCGACATCTTTGAGAAATTTGGAAGAAGAGAA

[0046] TTTCACCATGAAGGTGTGGAGGTGACGTTTCAGTGTTTTGTATATATCGTCATCATTGCAAACGAGGTG

[0047] GACGGGGTTCCTGGGTAGATAGCGAGCTGATATAAAACATCATTGGTGCAGTGACGTCCAAAACCACT

[0048] AAATGCATTGCCGGAATTATTTCGTATGTCCTGTACAATGAATGGACTTCTGTGTGGTCCAGGATGCGG

[0049] TTTGGATTGCAGCTTGCGAGCGACCAAGGGGTTTTTGGGTTTTTGGCGTTCCAAGGGGGACATAATCT

[0050] GATGGCTACTGCAATGCATGTGAACCAAAAAAAATGGTCATGGAAGGAATGAGTACGAGTGGGCGTA

[0051] CCCTTGGCGAAGAAAATATCCCTCTACAGTGAAGGTTGAATGTGGCAGGCTCATAATTTCCGCCTTGA

[0052] TTCTCTTCTTCTTCAGATTTTCCTCCTTCAGCTCCCAAACGTGCACACGTTTTTCCCATCGTTCAAACC

[0053] GCCGAACAACCACCTTTCGCCATTCGTCCAACGCCTGTTCAGCAAGCTTCACTTCGAGGCACCAGTC

[0054] TGGTCCACCTTTAAATGCATCAAATAACTTTGGCCATTCCTGTATTGTATAGAGGGGTCAGTCAGATGA

[0055] TCCTATGCAAGCCACTGTGACTTACAGGTGTATCGACATCGAAGTCCGGCTTATCCCAAACACGATCG

[0056] TGACTAATGATGTGGAGTCGCAAAAAATGAGAGAAGTCGGCAACAGCCCAAACCCAATCATCAGP2(768bp)

[0057] GTCGAAACTGAAACACCCCTGCAAGGCGGACTTAATGTCAGATAGTCTATTCATCCGCTGAGGGTCTC

[0058] GCAAGCCTCCTATCCTTCCGCTGATAACCTTTCAATTCCAGACAAGCCCTGTTTATCTGCCGCTGGTCT

[0059] CGAAACCTCATCGCTCCACATGCCAAGGTGAGTTTCAATCTATTTTTCGACACCCTGCACCGAGGACG

[0060] TATGCGAACACTGACATGTCCTTCATTGTAAAACAGTTTGCTCACCACGGATGGACCGTGCGTTGTGT

[0061] GAGGTGTTGTTACCGTACGGCCCAGCACAAGAGTGTTTATTCGATCATGATATAAATACGCACTGACGC

[0062] CCAACACATTTTGCTGCTTCACATGATGAATATCGACATGTACCATGCACATTTTGAAGACGTAGATATA

[0063] GGTAAAGTCCTGTTCAAACACGTTCCGTATGCTGGCTAAGAACCTATGCATTTCGCAGAGGAGTGGCG

[0064] GAATTTGATTGACGCTCTAGACAGTTCTCCGACCATTCCCGCCAGCAGTTACGACAAGCATTGCACCG

[0065] TTTGTGGACACGTCCAATCTGAACTGCTGAAGAGCGACCTAGGACTCGGTTTGATGGTTCCTGTCGTT

[0066] CCACATCCTGAACAACATCACAATGTTGAACAATCATTCATCCACTTGATCACCGACGAGGTTTTTGTA

[0067] TTATGCTGTGCATGCGCATCGCTTCGCAACAGCTCGCTGGCACAGGGAATAACATTTCATGTCCCTTGG

[0068] CAGTTTGAAGTCCT

[0069] P3(999 bp)

[0070] AACGGATCAACAACGAGGTCAAGGAGCATTGGGTGTCGTTGGCGAGGAGCGAGGAGCGACGCAGTC

[0071] CTCTGGTCCTCCAATATTACCTGTAACGCAGGAGGCTTCGCAATGACTGTGGCAATGTCATAAAGTTC

[0072] GACACTAGGAACGTAGGCTTCAGTCCAACAGAGGTAGAAACGAGAAGGTATGTGCTGGACGGCGTA

[0073] GGTGTGTGAAAGGGAGATGTCCGAGAAGCGGGCGGTTCAGACAGGCGGGTCCACCATAATTCCATCC

[0074] GCATTCAAACTCGTTTGTGGAGCGTGTCACCGGAAATGAGAGGATCGCTAGATAGCACAAAACAAAA

[0075] AATTGAGTTAAACCTGAGGACGTTCCAGAGCCTTGAGCATCATGGCCGCGGGGTAGGAGTGGTCCTT

[0076] GAGGAGCCGATGGGTGACGATAAGAAAGAGATGGTTAGTTTTCGACCCGGAACACCGGGATTCTGAG

[0077] CCGCCAAAGTTTATCATATCGGTCTTGGGCGCTTATCATCTTTTCTCGCGCGTCTGCGCCATCCGGCTCC

[0078] AAGTTCAAGTGAATGCGCCCACGACCATCGCACAGCCATGTGCCCGAGCCTCACCATACCAGGCAAA

[0079] CTCTTTCTGAATATGATCCGATAGCTGGCGTCTGAAAATGGAGGTGAGCAAGTCTTGTTGGTTCGAAA

[0080] TTCGAACTCTTGACGGTTTGTACAATGTTGTGCAGCTGATACGATGAGCACACGCGTGACAGGCCCGT

[0081] ATATGTGAGACCCACTTTTGCGTATCTGACTGGCTGTTGTCGGGCGCATGTGCGAAAAAGTGACTCTG

[0082] GCTGTGGGTTGGGGTCGTCGAACAGTCTACCTCAGGCAACCACGTTTGACGAACGTGGGATGACACA

[0083] AAGCGTCTGCATATGGCACTCAAATACATGGCCAAGCGTCGTTGTCGGTGAGAGCAGATCGGCAAATC

[0084] GGCAAGGCAATGGAGAGAGGAGGAACAGCGGATTCGCTTTTGCTTCTGGGTGTTC

[0085] P4(989bp)

[0086] CGTTCGTTGGTTGAGTGGTAAGAAGTGGGGTGCCTGATGCAGAGACGGCCCAGCCGCTGATGAAGTT

[0087] TGGTACTGAATATGTGTAGCCTGTTGCGGTGGGTAGCCCAAAGGAGAATGTGGGAACGGGCTGGAAT

[0088] AAACTCCAGGCTGAATAGGATATGACGAGTAATGGGACGACGGAAGTGGTGAGAAGTAGGGTACTGG

[0089] CTGCTGATATCGTGGAGTCTGTGGGTGCTGGAAGTGTGGTGGGTAGTACGGCGAGCAGGCAAATGGT

[0090] GGTGTGTATGATTGCGGAGGTAACGTCGAGTGTGGTGCATGGTATAAAACCGGAGGTTGTGGGGGTC

[0091] CGGACCATGGAGAAGAGGTGGTGTCAATTGGCCGTGAAGATGGCACCGGTGTCTCGTAAACTACTTG

[0092] ACCAGGCCGAAATACGGCTGGCGAATGAGATAGTTGAGAGGGCTGCGATGGCGGCTGTGAGGACGAT

[0093] AGCTGTGTCGGTGTTTGGAAGAGGTTGCGCATATGAAGAGCAGAAGCGCGTGGTGTTTTGTCGGCCT

[0094] GTCGTGGTGCGCGAGGAACAGGGGTGAAGACCGGTTGAGGGATGGGCTCCATCGCATCTGTGTTTTC

[0095] GTCAGCGCGATTCTTTTTTCGAGCTCTAGTTTGAGACGCTGGGGTATTCGAGACAGTAATGGCCAAAC

[0096] GCCAGTCATCAATAATTCCGGCGACCTCACGGAAGTCGCGGTCAGTCGCAAAATCCCAGGAGATTACT

[0097] TTCAGAATTTCGTGCTGTCCAATATTCGCACGCTTGAGGAAAGCACCGCTTCCGGAAACAAGTTTCTC

[0098] AAGCTGTTTCGGTGGCAAAGCAATCTCAGGTGCTAGAAAACAGCTATTTCCATGGCGCCGATGACGG

[0099] AGCAGCCTCCAATCAATCAGAAGGCTCCGAAGATGGTCCTTCTCGCCTTTTGAGATGGCTCGTGAACC

[0100] TTTGGGAGGCTCGAAGTTGATGGTAGCTTGGGAGACCTGTGAGACAT

[0101] P5(913bp)

[0102] GATGCCTGAGTGTTTGACGAAACTCTTGTAAGGTAGACTATTCCAGCTTCACATGAGAACAAAGTATG

[0103] GACTAAGATAAAAAGAGAAATACCTGTTGGGCAGCCAGTCTCGCCTCCTCCTCAGCGTCTAATTGCAC

[0104] AGCAACAGCAACAATAGGCGTCTTTCTAGGCCGGCCTGGTTTAGCAGACTTGGATGTCGATGCCGTCG

[0105] ATGCTGCCGTAGATGTAGAAGATGTCATAGACGTTGAAGTATTGGCCGACGTAATTTTGCCAGCGATG

[0106] GGCAGATCATTCTTGGTGGCTTCTTCAGGAGGGAATTTATTATGGGCAAAAGAGTCGCCGGTAAGGAT

[0107] ATCCGAAGCCAGGTGACGTGGGACGCGACCGTCAGTACTCTTCCGACTGCCAGCCAAATCCGGATGA

[0108] AAAGCATCGATGACTTCCCGTGAAATGACCAACAGAGTATTGACGAAGTCATTCTATATGAATGTAAG

[0109] TGGTAATTGGTAAGTCATTTACAATCTAAATCATGCATGATCGACTCACCACAACGAGGGAATGATCTT

[0110] CCCCAAGCAGCTCTGTCTGCCCTGTCCAAAAACGATACAGTATCTTCTGACGCTCCTCTCGTTGTTGA

[0111] GGTGTGACGTAGACGTCACTGAGAACGGGAATGGCTGCCAATGGAGGGTCGTTCAGCATGGTGAGA

[0112] ACTGAGGCTGAAACAGGCTGATTTGGACCCTGGACCCCAACGAACGATCCATGATGCGATCCATGAT

[0113] GTGATTGCGACTGAGTGAGTGGTTGTTCACCGCCATTATCCAGGGGGGTCGTATTTCTAGGCTGGAATA

[0114] TCGTGAGAACTTTGGTGGTATAACAAAAGGTCCACACAAACTTACTCTCTTTGGAGGACGATCAGATG

[0115] CCTGGTAGTCCTGGGACTGGTATGAAGACGA

[0116] P6(901 bp)

[0117] CTTACGCTTGCCTCTACCTGAATATGCAATATCTCCTGAGAATGCCTAGTCGGAGACGTTTCACTGGTA

[0118] TGAATGGTGAGCCTCTGGTCGTCAAGAAACTATATCTTTACTTACGGAGCTTTCGAAGGATACATTATT

[0119] TGCAAATGGATGTGGAAAAATCTCGAGTGCGAGAAAAATAATACCTACAGGACGTAAGTTTATCAAGT

[0120] CACTAGTCGTAGTTGTACAAACATTGACGGGAAATAGCTTGCCAGGAAACCTGATCAGGTTGAGGAA

[0121] GTATTCACGAGATGTCATGGCTATATGATGACGACCTTGATCCAGGTTTATACGCCCACACTTTCGAATT

[0122] CGCAGAGTACTCAAATCCTCATTTTAGATCTTAGGCCGAATTGTTAAAATTCTTTGGTCATGCGGTGCG

[0123] TGCGTGATTGATCAATTAGTCGGTCCTTGAAAGTCGAATGACTCACGATCCTTCATCAGTACTAACCAA

[0124] CCTCGAAGAGGTAAAGACGCTACTGCTATCTGGACAAAAGGTTCGACATCCTGTCCCAAAGAGTTGG

[0125] TTCTAACACGATTTTTGGATTATGCCTCCCAAACGTCGGTGTGAATGCACCGTGCATGGTTGTCAT

[0126] GACATTGAAACAGATGGGGAACGAGGCGTCTTAGTATCCTATGCGACGTGGAGGAGCCACCAGAAAG

[0127] ACGAGGAAACGAAGGATGTGCGCCTTTTAGCATCGGAAGTCGAAGCCAAAGCTCTCCTGGAACAGG

[0128] AGGAGACTTTTGAAGGCACTCCAGGAGATGTCAGTGGAAGATCTTGCGGCGATACCGACTGCAAG

[0129] AGAACGACCACTTCGAGAGGATCGGTACCAAATCGACAGAATACGCTCCATGGTTGCACACGCGAGT

[0130] GAAATCCAAGAGCGCAT

[0131] P7(965 bp)

[0132] CCGAGCCCCTTCTTTTTGAAACCGTGAAAAGATGCATGGTGCATGGGCCATGCGGACCAGGTTTTCCA

[0133] AACGCGCCATGTATGGTGAATGGAAAATGCTCTAAAGGCTATCCCAAAGCCTTTCAGTCATTTACGAC

[0134] CATGGATATGCACGGCTTTCCATTGTATTTTCGTCCAGATAATGGCGTAAAGGTCCTCATAGGTGGCGT

[0135] TTGGATCGACAATCGTTGGATTGTTCCTTTTTGCCCATTTCTCTCTGTTATGTTCGACTGCCATATCAAC

[0136] TGTGAATGCGCGGCTTCATTGGGCTCATTCAAATATCTCTTCAAGTACATCCAGAAGGGGGGAGATCG

[0137] TGCATCACTCGAGGTCGGTCAACATGACGAGATTCAGCAGTTCGTCAATGGACGGTACATCTCTGCTT

[0138] CGGAAGCTATCTACCGGATTTTCCATTTCGACACCCATGACCAGATTCCGAACATTGTCCGACTACAAG

[0139] TCCATCTTCCAGGCCGGCATATGGTAGTCTTTGACCCAACCGAAGATCCCGCAGTTCTTCTTGCACGG

[0140] GCAGTAAATGAGCGCACGCCGTTGACAGCATTCTTCGAAGCAAATGCTGATGAGGGGCTACTTGGAG

[0141] AGGAGGCTCGGAAGTACACCTACCAGGAGTTTCCTCAGCATTTTGTATGGAAGGATACAACAAAGAA

[0142] GTGGGCTGTCCGCCAGCGAGGCTTCGCATTGGGACGGATGTATTTTGTCTCACCGACCGGAGGGGAG

[0143] CGGTTCTATCTTCGGACCCTTCTAACAACTGTCAAGGGGCCACGTTCATTCGAGGATCTTCGAACGTA

[0144] CAATGGCGTCGTTCACAGCACATTTCATTCGGCCTGTCTTGCACATGGGCTGCTTGAGGATGATGGCG

[0145] AGTGGCGTCAATGTTTACAGGAGGCATCACATATGCAGACAGGAACCCGTCTACGCCAGCTGTTTGCG

[0146] ACCCTTCTTCTG

[0147] P8(846 bp)

[0148] GAGTAAAGCAACCGTCCACAAAATGCCACCCTCCCGGTAAGATGGGTCGACCGTCCATCATGATATTG

[0149] AGGCCAGTGAGATCCCTGTGGCATGTGATCGGTCGTTATAGCTTGAGAGATATGAAACGAGATTACCC

[0150] ATGAGCTACCAGATGACTATGCATAAAGCTAAGACCCTGGCATTACCTTAAATAACCGTGTAATAGTCC

[0151] CGAAGTGTTAACAACGCACCTCTAAGATCTGTGTAACAAAGTCGACAGCCTCTCCAACGGCGCCGAA

[0152] TTCTGGATCATCGAAAGGGCGAAGCGAGGGCATCACAAGATATTTAACGTCAGGTAGGAGGGGATCG

[0153] TTGAATGTCCCTAATATCTCGACGCAGTGATTTCGCGCGTCTTTGAGTCGTCCGCAGGAAAGGTATTGA

[0154] GCAATCTCCACTTCATCGGTTTTCTTTTGGATACGCTTTATGCATACAATGCTCCCATCCGAGAGCCTTT

[0155] TAGCATCCAGAACCCCTGGTATCTGCGAAATAAAATGTTAGTTAGATGGATGCTGCATCCAAATCACGT

[0156] TCAGGTTCTGCACGAACGATTTGTTGGATAGCATCTTCGCAGTATGTAGGATTAATAGAGGTTCCTATC

[0157] CATGATGGGGTCCAGTCAGGATGATATCGAGGTCGTAGTTCCAGGCCCTGCTGAAGAAAGAAGGAGT

[0158] ACCGATCTCGCCATAACTTCTCGATGTTGTCCAGTTCGGTTGGGTACACACGAAAAGTGATAGCAGGA

[0159] ATGACAGTGGACGGGGAGTTGCTTGATGTAGCATTCGCCTTTCCAGAGGAGGGCGTCGTATACGCGTC

[0160] GTGGCCTTGGATGGGTACACGAGAAGA

[0161] P9(976bp)

[0162] TGGATCTCTGTCCCTCGTTTCTGTGTGCCTGGTAGAAGTTGCTGCATGAGGTATATCATGGGCAAACCT

[0163] CCCATCTAGTACCCTTAGCTACCCCCAAACGATCCACCCGCAGTACCTCCTTCAGTTTTTATTTGACGC

[0164] GCATCCCTCTTTGTCAGTCTATATGGACATGTCCGACACTACTCTTATGCCCATATCCATATCTAACAAG

[0165] TTCTCTTTGAGCACTATCACCACCACCCTCACCGCTCCGACAGGGAACACCGCTCAGTCAGTCGCATT

[0166] TCGAGATGTGCTTACTTCCAATTCTGGCTCCAAACTGGAGATCAGCTCCGACTTCATGTTTACCATGGC

[0167] CCCCGCCCATATCCTTTCCGCCCCCGCCCGGAACACTGCTCATATCATTGCCTTTCAATTTATTCGCGCG

[0168] TGCTTTCTTTGGATTCCACCTCGTATCCTTTGTTTTCTGCACCGAATTTCATACTGCATCTCTAGCCAGG

[0169] TTCTATCCACGATAGCGATTACACCGACACTGCCTTGTCATAATTTGTTGATTGCTACTGTTGAGCTATT

[0170] GGTATAGATAAAGGTACAAGAAGAGTCTCCAGGAATAAAATGCGCTGTCCAAAACAAAAGGTAAAAT

[0171] TCAGTAACGGGAAGAACATATGATAAAATCTGGTGCAGGGAACTTGCTATAACACCTGTGTCATCGCC

[0172] AAAGATGATACTCTCAAGACCTATACAGAGTGAAGTTCAGAAGACCGATCAGACGAAGCAAAGCTGT

[0173] ATGCCTCGGCGCGAAGATCAAAACCATGCTCATTCATATCTTTTCTGACTTACCATAAGAACTTCCCTC

[0174] ACTGATTGTCGACCATTTCTAGTGCTTTCCAATCTTCAGCATTAAGCCAGGCTACCGTTGGAAGCATCA

[0175] CTTAGTACCAAACATTGCGGTTACCTCAAGCCATCGTTACCCTAAACGTGGTCCTCAGTCTCCGAGCC

[0176] ATCATTAACACCA

[0177] P10(986bp)

[0178] CCTCGCCTATGACTTTTGCTGCCCCTCTCGCTGAGGTATGGGCTTCTGCTAATACGTCTTCTGCAGCTG

[0179] TCAATTTCTTTCCGATACTGACGTGCCGCTGGACGCCTGTCCCCATCTTTGAGCAACAATCAATATCAA

[0180] TGACATCCTCCCTGGATAGCTCGCTATCCAGGAGTCCTGAGCACGCTCCTTGCCCTCGCCACCTTATTC

[0181] GAGCGAGACACGGTTCGGGAAGCTCGGACTGGGCGGAGCGTTTCCATCCGCACTCAACTCAGATGG

[0182] GCTGTATGTGTGCGTCCATGTTGGGGATGAGCGCAGAGGCTTTGAGGTGGTGTCGGTACAGCAAGAG

[0183] GAGTGTCGCCGGTGCGTAAACATCCATTGGGGGCTCGATATGTGAGTATTCTGATGTACAGTACCGTG

[0184] AGGCCGATCAGAACGTACGGAACGTTCATGGAGTCGGGAGCGAATTAGGATGAGTGCGACGTCTCTT

[0185] CTCTATTCCTTGTACTTCTTTCGTGGGGACTCCATGTAGTACATACCTGTAATGTAGTCCAATTCAATTC

[0186] TAACACATTTTTATGATATGTTGCGGAACAGCCGAAGCGGTTGCTTGAACTGTACTCAAGTTCACCAAT

[0187] TACCGCAACAACCACACTGACCACCACTACATACCACACAAAGACCTATTTATACGTCTTCTCGATTG

[0188] GGATCTGGCAGTGACCGGATGACCGCAGAAGGCCTCTAAGGCCCCTTTACCTTCCTACAAGGAAGGG

[0189] TACCTTCAAGGCTTCACGTGACCCACCACCGCGCTGCCAGGGGTGATGCATGGTTGGAATGGGAAGT

[0190] TTACAACTCACTGTTTTCGTCGTGGAGGTGCCCAGTACCGCTTTATGTGGGCACCACGGTTGTGGAGT

[0191] CTAAAGGCCGTCAAATGGTGGGGAGGATGGTCTTCAAGGGACCAGGTAAGGAGGGTATCACACATGC

[0192] GTGGCCATTTGTAGCTTACCATCGCTTGACAGGTG

[0193] P11(946bp)

[0194] GGCACACCAAGACACTAACAGTAATGAAGGTATTGCCGTACTTTCCTCATGTATCTCTCGATTGCTGAA

[0195] GTTCTCTCAACAGGAACCCAATACACGGAGGCCAGCGTTCAAGCCGCTGTTCAGACGATGAGCCAAA

[0196] TACAGAGTGTTCCACATGTGGGAAGCAGCCATGGTAAGTTTTCATATTTGCGAAGGGTGGCCTACATC

[0197] TGACAATCATCTTGATGACTATAGTAGGAAATACCGGCGCGAGCAGACCCAGTGATCGGCCATCAGGG

[0198] ACAGAACCGGACCAGCTTCAGGTTCCAACGTCAACACAGGTAAGTCATATCCCACGGAGACAAATAG

[0199] GTGTTACTGACAAAATGATCAGCTCAGCACTCGGTCTATTGCTCAACGTGCTCGCAGGCAAAGGGAA

[0200] CAGATGCAGCATGCCCAGGTCCAACAAAGCATGCACGAAGATAACCCGAGTAAGTGTATCTATTCCTT

[0201] CAATTCTCTCCTTATATCACTGATATAATTATCCCAAGATTCAAATGCCCGTTCACTGGCTCAACGTGCA

[0202] CGGCGTGAGAGGGAACGAGTCCAACGAGCTCAAGCACAATGGGAAGCCAATAGTAACGAAGGCAAG

[0203] ATTGTATGTTTCTCATCTCTTTAGTGATGGCTGAAACCTCCTCAGGAGAAACTCAACACATCGAGGCC

[0204] AATATTCAAGCTCCCACTCAAGGTGTAAGCCAGGATCAAACTATCCGACCGCCGCAAGTAGGAAATAG

[0205] CCAAGGCAAGTTGTCACACGATGAGATGACATACGTCACTCATGTCGTTTGAACAGTCATTTCCCTGC

[0206] CAGTTTCGCGTGCACGTCGGGAGAGGCCTCGCATGCACCGCAACTCACAGGAATCGGCCAATAACCA

[0207] TGTTGGTAAGTTTCCCTCAAGCAACTCGCTGCAATGTTAATACTTTTCGGACTCAACACTTTGCA

[0208] P12(958 bp)

[0209] ACATTGCTGGCGTAGTTCTCGAGATGGCCTTGCATAACTTCTGCGACAGCACCTTGATTGAGGAAACA

[0210] CCTGCTGTTTAGCAAAGCACAGCGAACCGGGAGTATAAACGCACTAGGTGTCCCGAGGGGTCTCCGC

[0211] AGCGCTCGTTCAAAGAGATGATGAAGAGGAGGGGAAAGTTATGGGATGAAAAAAAGGCGACCCAAT

[0212] GCTGGAAGGCAGCGGCTGACAGCTGGTGAATTATGTTCGAAGTTTGAGAAGCCCATCCTCGTGTCCT

[0213] GCTCGCAAAATAAGTCGTGAGGGCTCATGCAAATTACAGGCAGACTAAGTGCCGTGATGAGAGGGAA

[0214] GGCGGCGGAGGGCAGCGCAACAAGGGAGTGCATTTTCAAAATTGTAGCGATCATGTGGGGAAGTCAT

[0215] GTGATCTGAAGGACGCATCATCGGACAAGCTTGTGAGGGCTCGCGGTGCGCCTATCCGCTATTCGTAG

[0216] ATAGAAGAACCATAGGGCACTACTTAATAAGCTTCCCATGATGGCGCCGGACCCAAACCTAACTTGTG

[0217] CGCAATGGTACATTCCTCATGCAGTTCCCCACGAGCGTACTCCCAAGCATTGCCTCCCTCCTCATCTCC

[0218] CCAAACACTACTTATCGGGTCAGGGGAAGGCGTCCTTTCGGCTCCCCATGGATGATAGCGAGATGTTG

[0219] GGTCTGGGTCAAACGGTTCCACGTAATCGTCCGCCAAGCGCGCGAGCGCCTTCAATCTGTTTGAGCA

[0220] AGGGGGGAAAAAATTCATGGAGGAAGCTGCGTACCGCCGGGATCTTCCCCTGGTTATTTGCCTTGATG

[0221] TCCGATTCTTCAAGTACGACATCCCATTGGTGTCCCATGTTCAAGTATCTGCTCCCTCTTGCTGCGTTGT

[0222] ACCGAAAAATCTGGAGCTATCATGTACGGTTAGCAAAAACGTAGACTCAATCAGTCTGCCAAATACGC

[0223] ACTCTCCTGT

[0224] P13(979 bp)

[0225] CGTCTGAAGCGTGTCTATCCTGTTAGTTTATTGTACTTTTTTTGGTGGACGTGCATTGATTATTTCTGTT

[0226] CGCGTCCTACAGGGTTATTGTCCTTTCAATGGTGTTAGTACTGCTATTGTGCTCAAAGCTATCTGCTTTC

[0227] CAGCAAATTGGAACCAACTTTGGCCTAGCAAGGATCACGCCACTTGGTTGGAAGGTGCCCGCCTCAC

[0228] TGATTGCTATGATTTGAGCAAAGTCTTCACCTTCCGTGTGAGGAATACACATGCTCGCTTCCACGGTAT

[0229] GGTCTTTCAGCTATCCTGGTTTATATTCTGACCATTTCTCCAGCCTTTGACGTCACCCCGACCTTTTTGT

[0230] TGAGTCAAGACAATAACACTTGGCTTGGGCAACATTGGATTATGGGCAGGGCACTGACAAGAAGTGC

[0231] TTTTGCCAGTGATGACATCGAGAAGGTGTTCCATGACACGCTGCATGCCTTCTCCCACTTTACGTATGA

[0232] AATGAAGGACCACCTTGGCATTCATCTCGGGTTTCAGGGTGCGCAATGTCACTGCACTGCCGTCTGAA

[0233] TTAGCTGATCATTGAGACAGGCTTCTTGACATCCCATGGCCCTGTGATCTTCGACTGCACCACACATCT

[0234] TATGTATGCAGGTGTTTACCTGTATTGTCGATACTATGGTGACTAACTTGACGTTGTAGCTCTGATCCGC

[0235] ATGGCCATACCTACCTCGGAAATGGTGGTGAAGTGTGCGTTAACGAGTGGATTACTGGTCATTCCTGC

[0236] AATGCAATCTGCTCAGAGCTTGGGCTCCCCGACCTCTCTAATGTGAAAATTACACCGACTCATGGATTT

[0237] ATGTCTGATGACGACATCTGATCCTGTCAGTACGAACCGCTCATAAAAAATACTCTCGTTATTTAGTATT

[0238] TAATATACTACATTGGCGGGAAGATTGCTGACATCGGAATCATCCATGGTGAATGGCCTTTTGTCTCCA

[0239] ACTCGCAAACAACA

[0240] P14(802 bp)

[0241] TTGTATATGGGCCGCAACTCACATTATTCATATGGACCGGCTTGAGTAATGCTGCCTCGATCATGAGGC

[0242] GTATTTCTCGGGCTCCCATGAAATATTTTTCTCGCTTGGTGTGGGGAAGGTTAAATGCGAGGGCGAGA

[0243] GTCTCACGGGATTTACGACTCTGGTCTTCCCAGACACGATGTTGCGGATAATCATGTCGGGCATCATCG

[0244] CAGAATTCGATGATCTAGAAACTGTTCGGTAAAAAAAATTGCGCATTGAACGAAAGATTAATTCACCA

[0245] GACTGGTCATGTCCTTCATCAGGGTCGTAAAACTCGTGTACGTCATGCGCGTTGTGGGGTCAATTCTG

[0246] GACTGCGAGTTTGAGAGGACATGAGTAAGGAGATCAACTCCACGTTCTTGAGGGAAGAAGGGATTTT

[0247] TTTTTATTGAAAAACATGCCCACAGAAAAATAGAGCACCAGCAATTACCAAATAGGCCTTCCTGAGTG

[0248] AACTCTTTGCCAACGAGATGATTGTAGCCAGGTACTTCCTTGTGCATTCGGAGAAATTTTTCAAGCAG

[0249] TCTTGCTCTTCGTAAGCTTGTGGAAAAAGACCAAGTGATATATATATCGGCCACTTCTGAAATGATCTG

[0250] GTTGAGTTGTTCAAACGCCGGATTTTGGAGAGAGTTGACAAAGGCCAAATTGTAGGCTGTGGGATTT

[0251] TTACGACGGCTGGCCTTGGCCAGCTTCAGGCTTGGCTCGAGCTCTCCTGCGGCGGCCATTCCAGCGTT

[0252] CGATGGTGTGAGGGCGGAGACAGGGGGAAAAAGGAGTTGGCGAAAAGGAATGCP15(938bp)

[0253] GCAAGACCAACAAACCGAAGAGACAGGACACCTGCTGAAATGCTTGTGTCTTTGTTTCCCTCCAAAA

[0254] ATAGTAATAGTTCTGGTAGCATGTATTCGCCGCCTGGCGTTCGCAGATACTGGGGAGGAATTGGTGGG

[0255] ACCATACACCGGGAACGAGAAATATATCCCGACGCCTCTTGCTTGTCAAATATGTAACCCAAGATAGC

[0256] GCCTCGTATCAAGTGTGCCAAGAATCTGGGCTGCATGCCGAAGTCAAGCGTATAGGCAAGTTCACGGA

[0257] TCCAATCTTTTAAGACCTGCAGACCTCTCTGGAACTCAGGAATAAGGCTAACGCGGAGATTGACTTGT

[0258] GTCCCAAGTACGTGGAAAGCGGGCTTCAAGGCCTCGTAGAACCGATTGACCCAATGTCTGTAAAATA

[0259] CTTAGACCACGAGAGAAACTTGATTTATTAAAACTGGCCTTTTCTGGCTCCTCACATCGGACACGCTG

[0260] TTGACAGAGTGGGAAAAGTGTTTGAAGAATTAGCATCTGTTGAAGATGCACGCGGATACGCGCATTGTA

[0261] CCAATCATTTGTCAGAAGGGATGAATGTGTGTGTTCGTTGGGACATTCAATCCGATTACAGTAGCCAA

[0262] AGGCAATGAATGTGAGGCAAGGAGAGAACTGCGGGGATCTCTGGACGACCTCATTTTCTTCTGATATG

[0263] CGCTGCCGGATATGATCGCAAATGGCTCGTCTAAGGGTTCGCGATAAGTCCCAACCGGAAATGGAAAT

[0264] CTCCCCAAGTGAAGAATGTCGAGCACTGGGGAAATTAGTTTTCACCAGGTTGCGATAGAGGAAGGTA

[0265] TCGATTGGAATAATGAAGTCATCCCCAGAGGATCTACGGAAACCAAACATTTTTGCCACCGATGCCGT

[0266] AGTGAGAGGGTCGACTTTGAACGCGAGCTCATGAAGAAGGCGGACATAGTCAAGGAAP16(989bp)

[0267] CAATGTCTCCACAGCAAGGTTATTTTCCCAGAATTGAATTACACCAGTGCCTCATGACTCCGGGAAAC

[0268] AAACACATGATTCTTTTAGAACATAATGGATTGAGTTAAACTTCTGACAACGAATCAATCGTGGGTCA

[0269] AGACAGGAGCGAGCATTCAGGTAGGTAGGTACGTAGGTAGAGGTAGGTAGGTATCGCGGCATAGGCA

[0270] GGAACCCAAAGATCAAATGTGATCAGAAGAACCATACACTGGAAAATTGTTCGATAAGCGAGGTGAA

[0271] GCTGGAAAAACATATGGGAGAGAGTCAAAGCACGACGCAGCACCCAGAATAAAATAGATGCCCCTCC

[0272] GGCATCCAGCATCCAACGGTTGTAATCATGGCCCTCCGAGTACTGCTGACAAAACGAGAGGCTGGCA

[0273] AGGCTGGCCCTAACCTGGAGCTCAAATAGTAGTGGGGCCTGTTTGAGCAGTAAGGCGACATGGAGGT

[0274] ACGCACCAGCGCTGACACATCTAACTTTAAAGCCAGGCCAAACTAGGTTCGACAGAAGTAATGCCAG

[0275] CAGACTCGGAAATGGGAGGGGGAGGGGAAAAAATGGGTAAATGGTCGTCATAATGATTCTGCAACCG

[0276] TTCAAGCCGTCCTCGAGCATAGCGTTCTTCATACTTAAGGATGCTGGACTCCACGGTCGCCAACAGAC

[0277] TCCGGCGACGAATCTGATCATACTCCAAGGTGCGCAAAGCAAGTAGATCCGAAAGGGACCAAAACTC

[0278] TGGGTTGAATGGTGCATCCGCCGTAGTCCAAAGGGCGCCATCTTGGCTACACAGCTGGAGCAGCACC

[0279] GTTACATATGGATACATAAAAATTGACGCCAAGACCTACCCCTTCAGATGCATAGCCCCTGACTTTGTC

[0280] CATAAGCGATATAATTCTCTGTCTTGCATGGAGAATTCTTTCTGTAGAAACAGGTACCGTCCAACCAG

[0281] GTGATACAACGGATTGTCTCGCGGTTCGTTGGAGGTGAATAGCG

[0282] Example 3: Planting of the Agrocybe spp. strain 'Baiyu Mushroom 87':

[0283] The new variety of enoki mushrooms 'Nong Wanzhen No. 1' certified by Fujian Province was used as a control. 'Nong Wanzhen No. 1' is suitable for the cultivation of short-handled white jade mushrooms. The strains of 'Bai Yu Mushroom 87' and 'Nong Wanzhen No. 1' were activated and then used for original seed production. Bottle cultivation was adopted. The planting location was Fujian Wanchen Biotechnology Group Co., Ltd. The company's production method of growing white jade mushrooms was used for mushroom production management. It took 111 days from inoculation to harvest. 4 baskets and 64 bottles were randomly selected for single bottle weighing. The average single bottle fresh weight of 'Nong Wanzhen No. 1' was 275.1g, and the average single bottle fresh weight of 'Bai Yu Mushroom 87' was 349.5g. The single bottle yield of 'Bai Yu Mushroom 87' was much higher than that of 'Nong Wanzhen No. 1'.

[0284] Example 4: Batch planting of the species of Agrocybe 'Baiyu Mushroom 87':

[0285] The new variety of shiitake mushrooms recognized by Fujian Province, 'Nong Wanzhen No. 6', was used as a control. 'Nong Wanzhen No. 6' is a new variety selected and bred by the team in 2023 in cooperation with Fujian Wanchen Biotechnology Group Co., Ltd. This variety is also a shiitake mushroom strain suitable for short-stalked white jade mushroom cultivation. It has more advantages than 'Nong Wanzhen No. 1', so the better 'Nong Wanzhen No. 6' was selected as a control for batch planting. The production method of white jade mushroom cultivation by Fujian Wanchen Biotechnology Group Co., Ltd. was used for 4 consecutive batches of cultivation. The results are shown in Table 2. It can be seen from the table that the half-bottle fresh mushroom yield of 'Bai Yu Mushroom 87' was higher than that of 'Nong Wanzhen No. 6' in all 4 batches, and the average increase in yield in 4 batches was 6.7%, indicating that the hybrid variety 'Bai Yu Mushroom 87' is a high-yield variety. The average cap thickness and stipe length of 'Bai Yu Mushroom 87' are significantly different from those of the control variety 'Nong Wanzhen No. 6', while the cap diameter and stipe diameter are not significantly different. 'Bai Yu Mushroom 87' has good overall uniformity, thick mushroom stem, round mushroom cap, and overall white color, and has good commercial quality.

[0286] Table 2 Results of multiple batches of cultivation experiments

[0287]

[0288]

[0289] From the above, it can be known that in the present invention, the strain is obtained by hybridization and breeding with Agrocybe 'B02' and Agrocybe 'B06' as parents, and the fruiting body of 'Bai Yu Mushroom 87' has good overall uniformity, white color, round and thick mushroom cap, good hardness of mushroom stem, high yield, and has good development and application prospects.

[0290] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the term "comprise" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0291] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A species of Pleurotus eryngii suitable for producing Pleurotus eryngii, characterized in that: The strain: classified and named as Agaricus leucophylla 87, was deposited in the China Center for Type Culture Collection on November 7, 2024, with the collection number CCTCC M20242461, and the collection address is No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

2. The Agrocybe spp. strain suitable for producing Pleurotus eryngii according to claim 1, characterized in that: The strain is bred by hybridization with Pleurotus eryngii 'B02' and Pleurotus eryngii 'B06' as parents.

3. The mycelium produced by the Agrocybe spp. strain suitable for producing Pleurotus eryngii as claimed in claim 1.

4. The protoplasts produced by the Pleurotus eryngii strain suitable for producing Pleurotus eryngii as claimed in claim 1.

5. The fruiting body produced by the Pleurotus eryngii strain suitable for producing Pleurotus eryngii as claimed in claim 1.

6. A mushroom stick produced by the Agrocybe spp. strain suitable for producing Pleurotus eryngii as claimed in claim 1.

7. The use of a Pleurotus eryngii strain suitable for Pleurotus eryngii production as claimed in claim 1 in Pleurotus eryngii breeding, characterized in that: The application comprises the following steps: 1) Creation of breeding materials: Taking 'B02' and 'B06' as breeding parents, after the fruiting test, ejected the spores of the two parents when their fruiting bodies were mature, and spore germination was carried out. The germinated spores were picked up under a microscope and confirmed by lock-like union. The monospore strains without lock-like union were selected as breeding materials. Finally, 44 monospore strains of 'B02' and 19 monospore strains of 'B06' were obtained; (2) Hybridization pairing: 10 monospore strains of 'B02' and 'B06' with good growth were selected and paired in pairs, with a total of 100 combinations. After microscopic examination, 37 hybrids with lock-like unions were obtained, that is, 37 hybrids were obtained, of which 2 hybrid strains had poor growth in the mycelium stage and were discarded; (3) Hybrid screening: The 35 hybrid strains with normal growth obtained by hybridization were screened for fruiting test. Among them, 6 hybrid strains showed good fruiting performance in the initial screening, and these 6 were arranged for the re-screening experiment; (4) The six hybrids were screened for fruiting for the second time. All of them were able to produce mushrooms normally. However, there were differences in the properties and fresh weight per bottle of each hybrid strain. Among them, the hybrid with the fruiting number B0087 had the best overall properties. Its fruiting bodies were uniform, white, with thick stems, good hardness, and round caps. The fresh weight per bottle was 345g, which was much higher than the parent 'B06' suitable for Baiyu mushroom cultivation (single bottle fresh weight reached 270g), and 6.28% higher than the other parent 'B02' of 324.6g. Therefore, the hybrid Baiyu mushroom 87 inherited the advantages of high yield of the parent 'B02', and also inherited the characteristics of white fruiting bodies and thick stems of the parent 'B06'. The hybrid of the spotted jade mushroom B0087 was named Baiyu mushroom 87.

8. The use of a Pleurotus eryngii strain suitable for producing Pleurotus eryngii as claimed in claim 7, characterized in that: In the step 1, the mother culture medium for creating the breeding material is PDA culture medium, and the formula is: 200g potato, 20g glucose, 20g agar, and 1000mL water.

9. The use of a Pleurotus eryngii strain suitable for producing Pleurotus eryngii as claimed in claim 7, characterized in that: In the step 1, the formula of the culture medium used for original seed production and substitute planting is: 47% corn cobs, 25% rice bran, 7% bran, 2% cottonseed hulls, 5% bean skins, 4% active agent, 8% beet pulp, 2% husk ash, 70% water content, pH value 6.1-6.5; each bottle contains 243 grams of dry material.

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