Method for producing gastrodia elata symbiotic armillaria mellea by using liquorice dregs

Abstract

The invention discloses a method for producing gastrodia elata symbiotic armillaria mellea by using licorice root dregs, which comprises the following steps: mixing 20-40 parts by weight of licorice root dregs, 30-40 parts by weight of corn flour, 5-15 parts by weight of wheat bran, 20-30 parts by weight of cottonseed hull, 0.5-1.5 parts by weight of lime and 0.5-1.5 parts by weight of gypsum, uniformly stirring and mixing, adding water, soaking for 24 hours, bagging, sterilizing a fungus bag at high temperature and high pressure, cooling, and packaging into bags, thereby obtaining the gastrodia elata symbiotic armillaria mellea. Inoculating an armillaria mellea zymophyte solution, and culturing for 40 days at 15-25 DEG C under a dark condition, so as to obtain an armillaria mellea strain; the method is simple and easy to operate, secondary utilization is conducted on the waste traditional Chinese medicine liquorice dregs, the method is high in cultivation efficiency, the mycelial growth speed is higher than that of common fungus bags, the large-scale production requirement is met, the time cost can be greatly saved in industrial production, the production efficiency of armillaria mellea is improved, and the method is easy and convenient to operate and suitable for large-scale production. The method has important guiding significance on development and utilization of armillaria mellea.

Description

Technical Field

[0001] The invention relates to the field of culture and preparation of Armillaria mellea, and in particular to a method for producing Gastrodia elata symbiotic Armillaria mellea by utilizing liquorice herb residue. Background Art

[0002] Licorice is a Qi-tonifying medicine, which is the dried root and rhizome of the leguminous plants Glycyrrhiza uralensis, Glycyrrhiza uralensis or Glycyrrhiza glabra. Licorice is sweet, nourishing and moistening. It is cool when raw and warm when roasted. It mainly enters the spleen and lung meridians, and also enters the stomach and heart meridians. It can not only replenish Qi and replenish the middle, but also relieve pain, moderate the medicinal properties, and also eliminate phlegm, relieve cough, and detoxify. Honey roasting has strong Qi-tonifying and relieving effects; raw use can purge fire and detoxify; it contains triterpenoid saponins and flavonoids.

[0003] Armillaria fungus ( Armillaria ) is also known as hazel mushroom, Zhen mushroom, honey mushroom, belongs to the kingdom of fungi, phylum Basidiomycetes, class Basidiomycetes, order Agaricales, family Trichoderma, genus Armillaria, and is a medicinal and edible fungus. Armillaria mainly lives a saprophytic life and also lives a parasitic life. It has a symbiotic relationship with Gastrodia elata. As a medicinal and edible fungus, Armillaria elata has extremely high economic and medicinal value, and high-quality Armillaria elata is the key to producing high-quality Gastrodia elata. During the growth and development of Gastrodia elata, Armillaria elata is crucial to its survival and directly affects the growth and yield of Gastrodia elata. Gastrodia elata has no chlorophyll, and the loss of photosynthesis function makes it only able to use the nutrition brought by Armillaria elata after invading itself. The two use nutrition from each other to achieve the purpose of symbiosis. Therefore, Armillaria elata is an important symbiotic fungus that is indispensable in the cultivation of Gastrodia elata.

[0004] Due to the important role that Armillaria mellea plays in human health, the breeding of Armillaria mellea under natural conditions is far from meeting people's needs. Therefore, artificial cultivation has always been a hot topic of concern. At present, the artificial cultivation of Armillaria mellea original species mostly adopts the method of sterilizing branches and twigs and then making strain bags for inoculation. However, the common cultivation method has high material and labor costs and is time-consuming. As the cultivation cycle becomes longer, the risk of contamination by other microorganisms during the artificial cultivation of Armillaria mellea is greatly increased. Not only is the production efficiency low, it is difficult to adapt to the requirements of industrialization, but it is also easy to obtain aged Armillaria mellea, which seriously affects the quality of Armillaria mellea. Summary of the invention

[0005] In view of this, the present invention provides a method for producing Gastrodia elata symbiotic Armillaria mellea using liquorice root residues. The method uses the Chinese medicinal liquorice root residues discarded by the factory to make a fungus bag culture medium to cultivate Armillaria mellea. By further optimizing the process, the optimal nutrients and proportions for culturing and growing Armillaria mellea with the liquorice root residues are obtained, thereby achieving the purpose of increasing the yield and shortening the growth cycle. Using the Chinese medicinal liquorice root residues to cultivate Armillaria mellea can save forest resources and achieve sustainable development, and can provide a reference for the factory production of Armillaria mellea.

[0006] The method for producing Gastrodia elata symbiotic Armillaria melleae from liquorice root residue of the present invention is as follows: 1. Prepare culture medium Mix 20-40 parts by weight of liquorice residue, 30-40 parts by weight of cornmeal, 5-15 parts by weight of wheat bran, 20-30 parts by weight of cottonseed hulls, 0.5-1.5 parts by weight of lime and 0.5-1.5 parts by weight of gypsum, stir and mix, soak in water for 24 hours and then bag, the water content of the mixture after bagging is 50-70%, sterilize the bags at high temperature and high pressure (sterilize at 121°C for 2-4 hours), and cool; 2. When the temperature of the fungus bag drops below 28 ℃, spray the cooled fungus bag with alcohol, place it in a clean bench for UV sterilization for 25-35 min, inoculate it with Armillaria fermentation liquid, and culture it at 15-25 ℃ in the dark for 40 days to obtain the Armillaria strain.

[0007] The method of the present invention has the following characteristics: (1) The present invention uses Chinese medicinal liquorice residue to prepare the culture medium in the bag, which can significantly improve the production efficiency of Armillaria mellea. The method for producing Armillaria mellea is faster than the conventional method, which can shorten the industrial production time. The Armillaria mellea prepared by the method can grow to a full bag within 40 days. (2) The method of the present invention can improve the quality of the honey fungus produced by the honey fungus bag. The mycelium germination can be observed in the bag prepared by the method 4 days after inoculation. Mycelium begins to grow in the places in contact with the material, and the mycelium is white; the mycelium is relatively thick, has vigorous branches, good ductility, and eats the material quickly; it has the advantages of strong resistance to foreign bacteria and good stress resistance; The method for producing Gastrodia elata symbiotic Armillaria mellea with liquorice herb residues of the present invention is simple, easy to operate, and low in cost. The discarded Chinese medicinal liquorice herb residues are reused for the second time, which belongs to the reuse of resources. The method has high cultivation efficiency, and the mycelium growth rate is faster than that of ordinary mushroom bags, which is suitable for large-scale production needs. The prepared Armillaria mellea has the advantages of strong resistance to foreign bacteria, good stress resistance, fast mycelium growth rate, etc. The present invention can greatly save time cost and improve the production efficiency of Armillaria mellea in industrial production. The operation method of the present invention is simple, has important guiding significance for the development and utilization of Armillaria mellea, and is suitable for industrial-scale production and market promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] Figure 1 The growth of fungus bags prepared by the method of the present invention; Figure 2 The growth conditions of mushroom bags made by the conventional method and the method of the present invention are compared. The first mushroom bag in the figure is made by the conventional method, and the second to fourth are mushroom bags of Examples 1 to 3. DETAILED DESCRIPTION

[0009] The technical scheme of the present invention is further described in detail below through examples, but the content of the present invention is not limited thereto. The methods in the present examples are conventional methods unless otherwise specified, and the materials and reagents used are obtained from commercial sources unless otherwise specified; The Armillaria fermented liquid is prepared by inoculating Armillaria into a liquid culture medium (1% glucose, 2% sucrose, 3% silkworm pupa powder, 0.075% MgSO4·7H2O, 0.15% KH2PO4, 1% ethanol) and fermenting at 25°C for 8 days.

[0010] Example 1: Production of Gastrodia elata symbiotic Armillaria mellea with liquorice root residue 1. Mix 30 parts of licorice residue, 35 parts of cornmeal, 10 parts of wheat bran, 25 parts of cottonseed hulls, 0.5 parts of lime and 0.5 parts of gypsum, and stir evenly; add water to the mixture and soak it for 24 hours, then put it into a polypropylene spawn bag (15×30 cm) to make a spawn bag, and add an appropriate amount of soaking liquid to each spawn bag to make the water content of the mixture in the spawn bag 60%; 2. Use a high temperature and high pressure autoclave to sterilize the bags at 121°C for 4 hours, and set aside after cooling; 3. When the temperature of the fungus bag drops below 28°C, spray the cooled fungus bag with alcohol and place it in a clean bench for UV sterilization for 30 min. Take the honey fungus fermentation liquid and inoculate it. The inoculation volume of each fungus bag is 20 mL, and the inoculation is completed on the bench. 5. Place the inoculated fungus bag in a dark room at 15-25°C for 40 days to grow fully, and you will get the honey fungus strain; Figure 1 The growth status of the fungus bag after 20 days of inoculation. Figure 1 It can be seen that using licorice residue to produce Gastrodia elata symbiotic Armillaria can increase the growth rate of Armillaria, and the mycelium is thicker and more branched, and is not easily contaminated by Trichoderma viride.

[0011] Example 2: Production of Gastrodia elata symbiotic Armillaria mellea from liquorice root residue 1. Mix 25 parts of licorice residue, 35 parts of cornmeal, 10 parts of wheat bran, 25 parts of cottonseed hulls, 0.5 parts of lime and 0.5 parts of gypsum, and stir evenly; add water to the mixture and soak it for 24 hours, then put it into a polypropylene spawn bag (15×30 cm) to make a spawn bag, and add an appropriate amount of soaking liquid to each spawn bag to make the water content of the mixture in the spawn bag 60%; 2. Use a high temperature and high pressure sterilizer to sterilize the fungus bag at 121°C for 3 hours, and set aside after cooling; 3. When the temperature of the fungus bag drops below 28°C, spray the cooled fungus bag with alcohol and place it in a clean bench for UV sterilization for 30 min. Take the honey fungus fermentation liquid and inoculate it. The inoculation volume of each fungus bag is 20 mL, and the inoculation is completed on the bench. 5. Place the inoculated fungus bag in a dark room at 15-25°C for 40 days to grow fully, and you will get the honey fungus strain; Example 3: Production of Gastrodia elata symbiotic Armillaria mellea with liquorice root residue 1. Mix 20 parts of licorice residue, 35 parts of cornmeal, 10 parts of wheat bran, 25 parts of cottonseed hulls, 0.5 parts of lime and 0.5 parts of gypsum, and stir evenly; add water to the mixture and soak it for 24 hours, then put it into a polypropylene spawn bag (15×30 cm) to make a spawn bag, and add an appropriate amount of soaking liquid to each spawn bag to make the water content of the mixture in the spawn bag 60%; 2. Use a high temperature and high pressure sterilizer to sterilize the fungus bag at 121°C for 3 hours, and set aside after cooling; 3. When the temperature of the fungus bag drops below 28°C, spray the cooled fungus bag with alcohol and place it in a clean bench for UV sterilization for 30 minutes. Take the honey fungus fermentation liquid and inoculate it. The inoculation volume of each fungus bag is 20mL, and the inoculation is completed on the bench. 5. Place the inoculated fungus bag in a dark room at 15-25°C for 40 days to grow fully, and you will get the honey fungus strain.

[0012] Comparative Example 1: Conventional method for producing Gastrodia elata symbiotic Armillaria 1. Mix 30 parts of sawdust, 35 parts of cornmeal, 10 parts of wheat bran, 25 parts of cottonseed hulls, 0.5 parts of lime and 0.5 parts of gypsum, and stir evenly; add water to the mixture and soak it for 24 hours, then put it into a polypropylene spawn bag (15×30 cm) to make a spawn bag, and add an appropriate amount of soaking liquid to each spawn bag to make the water content of the mixture in the spawn bag 60%; 2. Use a high temperature and high pressure autoclave to sterilize the bags at 121°C for 4 hours, and set aside after cooling; 3. When the temperature of the fungus bag drops below 28°C, spray the cooled fungus bag with alcohol and place it in a clean bench for UV sterilization for 30 minutes. Take the honey fungus fermentation liquid and inoculate it. The inoculation volume of each fungus bag is controlled at 20mL and the inoculation is completed on the bench. 5. Cultivate the inoculated fungus bag at 15-25℃ in the dark room for 45 days to get full growth of honey fungus. Figure 2 , the figure shows the cultivation situation on the 20th day, and it can be seen that the mycelium of Armillaria produced by the conventional method is white, but the growth rate of the mycelium is slower than that of Example 1-3.

Claims

1. A method for producing Gastrodia elata symbiotic Armillaria mellea using liquorice root residues, characterized in that: Mix 20-40 parts by weight of licorice residue, 30-40 parts by weight of cornmeal, 5-15 parts by weight of wheat bran, 20-30 parts by weight of cottonseed hulls, 0.5-1.5 parts by weight of lime and 0.5-1.5 parts by weight of gypsum, stir and mix well, soak in water for 24 hours and then pack into bags, sterilize the bags with high temperature and high pressure, inoculate with Armillaria fermentation liquid after cooling, and culture at 15-25°C in the dark for 40 days to obtain Armillaria strain.

2. The method for producing Gastrodia elata symbiotic Armillaria mellea using liquorice root residue according to claim 1, characterized in that: The moisture content of the mixture after bagging is 50-70%.

3. The method for producing Gastrodia elata symbiotic Armillaria mellea using liquorice root residue according to claim 1, characterized in that: High temperature and high pressure sterilization is sterilization at 121 ℃ for 2-4 hours.

4. The method for producing Gastrodia elata symbiotic Armillaria mellea using liquorice root residue according to claim 1, characterized in that: Before inoculation, the bacteria bags were sprayed with alcohol and sterilized by ultraviolet light in a clean bench for 25-35 minutes.

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