Dry dyeing method

Abstract

The invention relates to a dry dyeing method which comprises the following steps: S1, placing a slide to be dyed in a slide bin on a centrifugal dyeing disc; s2, rotating the centrifugal dyeing disc, starting a dyeing liquid assembly, and spraying a dyeing liquid towards the direction of the slide by using a liquid spraying head at the tail end of the dyeing liquid assembly; s3, after the dyeing liquid is sprayed, washing is not needed after the reaction is completed, then the centrifugal dyeing disc rotates at a high speed for a certain time, and the redundant dyeing liquid on the slide is removed under the centrifugal effect. According to the staining method, after the staining solution is injected to the upper side of the slide, the staining solution on the slide is directly thrown away in a high-speed centrifugation mode without a washing step. By omitting the washing step, the time is saved, and the overall dyeing efficiency can be greatly improved. Meanwhile, the integral structure of the dyeing machine is simplified, and the manufacturing cost is reduced.

Description

Technical Field

[0001] The invention relates to the field of biochemical staining, and in particular to a dry staining method. Background Art

[0002] In the observation of bacterial morphology, it is necessary to use the staining method to color the bacteria and increase the light and dark contrast with the background, so that the individual morphology and partial structure can be observed more clearly under an optical microscope. Bacterial staining methods are divided into: single staining method and double staining method.

[0003] For example, the invention patent with application publication number CN110286023A discloses an automatic constant temperature high-definition staining device, which places the glass slide in a staining chamber, which is connected to a variety of staining liquid pipelines, and can perform automatic staining operations with a variety of staining liquids as needed. This staining chamber structure is stationary, and the glass slide is placed and stained before being taken out. There is also a centrifugal staining machine, which includes a staining tray, and the staining tray has several glass slide chambers distributed along the circumference for placing glass slides. The staining tray is rotatable, and during the rotation of the staining tray, the staining efficiency can be improved by spraying the staining liquid onto the glass slide with a nozzle. However, after each staining liquid is sprayed, the excess staining liquid needs to be sprayed on the glass slide area through a water nozzle for rinsing; more processes, in turn, reduce efficiency. The glass slides must also be dried before reading the film, and the drying time of the glass slides after being rinsed with clean water is also extended. Summary of the invention

[0004] The object of the present invention is to provide a dry dyeing method to improve dyeing efficiency.

[0005] To achieve the above object, the present invention adopts the following technical solution:

[0006] A dry staining method comprises the following steps: S1: placing a slide to be stained in a slide bin located on a centrifugal staining disk;

[0007] S2: The centrifugal staining disk rotates, the staining liquid assembly is started, and the staining liquid is sprayed toward the glass slide using the spray head at the end of the staining liquid assembly;

[0008] S3: After spraying the staining solution, wait for the reaction. After the reaction is completed, no rinsing is required. Then the centrifugal staining disk is rotated at high speed for a certain period of time to remove excess staining solution on the glass slide under the action of centrifugation.

[0009] Furthermore, in step S2, there are more than two types of dyeing liquids, and different dyeing liquids are located in different dyeing bottles and have corresponding different liquid spray heads; then step S3 is performed once after each dyeing liquid is sprayed.

[0010] Furthermore, the slide chambers are provided with a plurality of slide chambers distributed along the circumference of the centrifugal staining disk.

[0011] Furthermore, when placing the glass slide, one end of the glass slide is firstly abutted against the fixed limiting portion of the glass slide bin, and then the other end is abutted against the elastic limiting portion of the glass slide bin.

[0012] Furthermore, the elastic limiting portion includes a slider and a spring, one side of the slider is connected to the spring, and the other side opposite to the slider forms a limiting surface for the other end of the glass slide to abut against.

[0013] Furthermore, a support portion is provided at the bottom of the glass slide bin, and the bottom side of the inserted glass slide is supported by the support portion, so that there is a gap between the bottom side of the glass slide and the bin bottom.

[0014] Furthermore, the centrifugal staining disc is located in the cylinder, the upper part of the cylinder is provided with the liquid spray head, the staining liquid assembly includes a liquid supply pipeline, a liquid pump and a staining liquid bottle, and the liquid pump pumps the corresponding staining liquid into the liquid spray head.

[0015] Beneficial effects of the present invention:

[0016] In the present invention, after the dyeing liquid is sprayed onto the upper side of the glass slide and the reaction is completed, the dyeing liquid on the glass slide is directly thrown out by high-speed centrifugation without a rinsing step. Under the action of high-speed centrifugation, the glass slide is basically dried and no excess dyeing liquid remains. Therefore, after reducing the rinsing step, time is saved and the overall dyeing efficiency can be greatly improved. At the same time, the structural components related to rinsing are omitted, the overall structure of the dyeing machine is simplified, and the manufacturing cost is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is an outline diagram of a dyeing machine for implementing the dry dyeing method of the present invention;

[0018] Figure 2 yes Figure 1 The internal structure diagram of the dyeing machine shown;

[0019] Figure 3 This is a diagram of the structure of a centrifugal staining disk;

[0020] Figure 4 yes Figure 3 A partial view of the figure (one cover is omitted).

[0021] The names corresponding to the marks in the figure are:

[0022] 1. Outer shell; 11. Touch screen; 12. Top cover; 21. Staining liquid bottle; 22. Liquid pump; 23. Electric valve; 24. Spray head; 3. Cylinder; 41. Hot air blower; 42. Hot air blowpipe; 43. Temperature sensor; 5. Centrifugal staining plate; 51. Slide; 6. Slide bin; 61. Fixed limit part; 62. Elastic limit part; 621. Cover plate; 622. Sliding block; 623. Spring; 63. Support part; 7. Slide. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0024] Example:

[0025] To facilitate understanding of the dyeing method of the present invention, a dyeing machine capable of implementing the method is first introduced.

[0026] like Figure 1-Figure 4 As shown, the dyeing machine includes a shell 1, and a slope is provided on the front side of the shell 1. A touch screen 11 is provided on the slope, which can be connected to a control system. The operation of the dyeing machine can be controlled and some parameters, such as working time, internal temperature, etc., can be displayed by corresponding buttons on the touch screen 11.

[0027] Figure 2 and 3 As shown, a cylinder 3 is provided in the housing, a centrifugal staining disk 5 is provided in the cylinder 3, and a plurality of slide bins 6 are distributed along the circumference of the centrifugal staining disk 5. In this embodiment, 8 slide bins 6 are provided, and the number can be increased or decreased according to the situation in other embodiments. Figure 3 As shown, in order to facilitate understanding of the structural design, seven slides 7 are installed, and another slide chamber 6 is empty. A driving mechanism is provided at the bottom of the cylinder 3, including a motor, which is used to drive the centrifugal staining disk 5 to rotate. This turntable structure is very mature and will not be described in detail. A servo motor can be used. A top cover 12 is provided on the upper side of the housing 1. One side of the top cover 12 is hinged. A handle is also provided on the top cover 12. The operator can hold the handle to flip the top cover 12 up and open it to perform related operations. The top cover 12 is covered during the staining process.

[0028] A dyeing liquid assembly is arranged in the shell, and the dyeing liquid assembly includes a liquid supply pipeline, a dyeing liquid bottle 21 , and a liquid pump. The liquid pump pumps the corresponding dyeing liquid into the liquid spray head 24 . Figure 2 In the structure shown, the dyeing liquid assembly includes six groups, each group includes a dyeing liquid bottle 21, a liquid pump 22, a liquid supply pipeline, and a liquid spray head 24. The liquid pump can be a peristaltic pump, but is not limited to this. The liquid spray head 24 is arranged on the upper part of the barrel 3 and can spray the dyeing liquid toward the glass slide area in the barrel 3. Each dyeing liquid bottle 21 can be filled with a dyeing liquid as needed, and the dyeing liquid bottles 21 are numbered. When dyeing different samples, one or more dyeing liquids can be selected in combination with the corresponding dyeing process. The outlet of each pump is connected with an electric valve 23, such as a pipe clamp valve, which is used for switching and adjusting the flow.

[0029] The slide chamber 6 has a rectangular trough body, which can accommodate a slide. A support portion 63 is provided at the bottom of the trough body, and the bottom of the inserted slide is supported by the support portion 63, so that there is a gap between the bottom of the slide and the bottom of the chamber. The support portion 63 is provided with two groups, one group is a circular protrusion and the other group is a strip protrusion along the length direction of the slide chamber 6. The thickness of the support portion 63 is less than 1 mm, preferably 0.5 mm or a value thereabout.

[0030] The outer end of the slide chamber 6 is provided with a fixed stopper 61, and the inner end (the end on the centrifugal staining disk 5) is provided with an elastic stopper 62. When placing the slide, the side with the sample faces upward, and one end of the slide 7 is firstly abutted against the fixed stopper 61 of the slide chamber 6, and then the other end is abutted against the elastic stopper 62 of the slide chamber 6, and the slide 7 is clamped by the elastic stopper 62 to prevent it from falling off during centrifugal movement.

[0031] Figure 3 and 4 As shown, the elastic limiting portion 62 includes a slider 622 and a spring 623. One side of the slider 622 is connected to the spring 623, and the other side opposite to the slider forms a limiting surface for the other end of the glass slide 7 to abut against. The slider 622 is L-shaped, with one end extending vertically into the glass slide bin 6, and the other end being horizontally arranged and connected to one end of the spring 623. A slide groove 51 is provided on the centrifugal staining tray 5, for the horizontal end of the slider 622 to extend into, and the spring 623 is located in the slide groove 51. When the slider 622 is subjected to force, it can move in the length direction of the glass slide bin 6. A cover plate 621 is also provided on the upper side of the slide groove 51, and the cover plate 621 is used to shield the spring 623 area. The cover plate 621 is fixed to the upper side of the centrifugal staining tray 5 by screws.

[0032] Figure 2 As shown, the dyeing machine is also equipped with a drying module, which can be used as an option. The drying module includes a hot air blower 41 and a hot air blowpipe 42 connected thereto. One end of the hot air blowpipe 42 is arranged on the cylinder 3, and can blow hot air toward the cylinder 3. During the dyeing process, the cylinder 3 is in a certain suitable temperature range, which is conducive to improving the dyeing effect. The temperature sensor 43 installed on the cylinder 3 is used to measure the temperature inside the cylinder 3 and feed it back to the control system to adjust the hot air blower 41.

[0033] A dry staining method comprises the following steps: S1: placing a glass slide 7 to be stained in a glass slide bin 6 located on a centrifugal staining disk 5.

[0034] The side of the glass slide 7 coated with the sample faces upward, and one end of the glass slide 7 is firstly abutted against the fixed stopper 61 of the glass slide chamber 6, and then the other end is abutted against the elastic stopper 62 of the glass slide chamber 6. The bottom side of the glass slide is supported and matched with the support part 63 in the glass slide chamber 6. After each glass slide is fixed, the staining operation is performed.

[0035] S2: The centrifugal staining disk 5 rotates, the staining liquid assembly is started, and the staining liquid is sprayed toward the glass slide 7 by the liquid spray head 24 at the end of the staining liquid assembly. The centrifugal staining disk 5 rotates to align with the corresponding glass slide area, and the rotation angle of the centrifugal staining disk 5 can be controlled by a servo motor according to the number of glass slides and the interval angle of the glass slide bin.

[0036] S3: After the dyeing solution is injected, wait for the reaction. After the reaction is completed, no rinsing is required. Then the centrifugal staining disk 5 is rotated at high speed for a certain period of time to remove the excess dyeing solution on the glass slide under the centrifugal effect. The rotation speed of the centrifugal staining disk 5 in this step should be greater than that in step S2. The specific rotation speed is not limited, as long as the centrifugal drying effect is achieved. The rinsing can be carried out by water rinsing or other existing rinsing liquids.

[0037] If more than two kinds of staining solutions are required for staining the sample, step S3 is performed once after each staining solution is sprayed in step S2.

[0038] In the present invention, the flushing step is omitted, the process is reduced, and the overall dyeing efficiency can be greatly improved. At the same time, the structural components related to flushing are omitted, the overall structure of the dyeing machine is simplified, and the manufacturing cost is reduced.

[0039] The centrifugal staining disk 5 is located in the cylinder 3, and the spray head 24 is located in the upper part of the cylinder 3. After the spray head 24 sprays the staining liquid, most of the staining liquid is sprayed onto the glass slide area, and a small amount of staining liquid or the staining liquid thrown out in step S3 flows to the bottom of the cylinder 3. A waste liquid collection pipe is connected to the bottom of the cylinder 3 to discharge the waste liquid for subsequent centralized treatment.

[0040] The present invention is not limited to the above-mentioned optimal implementation mode. Anyone can derive other various forms of products under the inspiration of the present invention. However, no matter what changes are made in the shape or structure, all technical solutions that are the same or similar to those of the present application fall within the protection scope of the present invention.

Claims

1. A dry dyeing method, characterized in that: The method comprises the following steps: S1: placing a slide to be stained in a slide bin located on a centrifugal staining disk; S2: The centrifugal staining disk rotates, the staining liquid assembly is started, and the staining liquid is sprayed toward the glass slide using the spray head at the end of the staining liquid assembly; S3: After spraying the staining solution, wait for the reaction. After the reaction is completed, no rinsing is required. Then the centrifugal staining disk is rotated at high speed for a certain period of time to remove excess staining solution on the glass slide under the action of centrifugation.

2. A dry dyeing method according to claim 1, characterized in that: In step S2, there are two or more types of dyeing liquids, and different dyeing liquids are located in different dyeing bottles and have corresponding different liquid spray heads; After each dyeing liquid is sprayed, step S3 is performed once.

3. A dry dyeing method according to claim 1, characterized in that: The slide chambers are provided with a plurality of slide chambers distributed along the circumference of the centrifugal staining disk.

4. A dry dyeing method according to claim 1, characterized in that: When placing the glass slide, one end of the glass slide is firstly abutted against the fixed limiting portion of the glass slide bin, and then the other end is abutted against the elastic limiting portion of the glass slide bin.

5. A dry dyeing method according to claim 4, characterized in that: The elastic limiting part comprises a slider and a spring. One side of the slider is connected to the spring, and the other side opposite thereto forms a limiting surface for the other end of the glass slide to abut against.

6. A dry dyeing method according to claim 4, characterized in that: The bottom of the glass slide bin is provided with a support portion, and the bottom side of the inserted glass slide is supported by the support portion, so that there is a gap between the bottom side of the glass slide and the bin bottom.

7. A dry dyeing method according to claim 1, characterized in that: The centrifugal dyeing disc is located in the cylinder, the upper part of the cylinder is provided with the liquid spray head, the dyeing liquid assembly comprises a liquid supply pipeline, a liquid pump and a dyeing liquid bottle, and the liquid pump pumps the corresponding dyeing liquid into the liquid spray head.

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