Ginseng extract composition as well as preparation method and application thereof

Abstract

The invention discloses a ginseng extract composition and a preparation method and application thereof, and belongs to the field of extraction technology.The preparation method comprises the steps that an enzyme-containing crude extract is extracted from fresh ginseng containing fresh stems and leaves, main root total saponins are extracted from main roots of ginseng, fibrous root total saponins are extracted from fibrous roots of ginseng, and the ginseng extract composition is obtained. And dissolving and mixing the enzyme-containing crude extract, the main root total saponins and the fibrous root total saponins by using a buffer solution, converting for enough preset time at the appropriate temperature of the enzyme to obtain a crude product system, and removing water and water-soluble impurities in the crude product system to obtain the ginseng extract composition. According to the ginseng extract composition, enzymes in fresh ginseng and saponin in main roots and fibrous roots are combined, conversion and extraction of ginsenoside are promoted, natural enzymes in the fresh ginseng are beneficial to converting the saponin into components with higher biological activity, and the prepared ginseng extract composition not only can treat the alcoholic fatty liver, but also has a prevention effect on the alcoholic fatty liver; and compared with direct taking of ginseng or taking of a direct extract of the whole ginseng, the effect is better.

Description

Technical Field

[0001] The invention relates to a ginseng extraction composition and a preparation method and application thereof, belonging to the field of extraction technology. Background Art

[0002] Excessive drinking, through ethanol itself and its derivative acetaldehyde, can cause repeated fatty degeneration, necrosis and regeneration of liver cells, leading to alcoholic liver disease, such as alcoholic fatty liver. Starting from the initial simple alcoholic fatty liver, if not intervened, the disease may gradually develop into alcoholic hepatitis, and then liver fibrosis, and eventually may move towards the serious stage of alcoholic cirrhosis.

[0003] Common drugs for treating alcoholic fatty liver include Silybin Capsules, Liver Protection Capsules, Compound Yiganling Capsules, and Kaixilai Tablets, but these drugs may cause adverse reactions such as dizziness and vomiting. For patients with allergic constitutions, allergic reactions may occur. Long-term high-dose use may cause abnormal liver function. Therefore, a drug or food that has both preventive and therapeutic effects on alcoholic fatty liver and no toxic side effects is needed to prevent or improve the pain caused by alcoholic fatty liver. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, the present invention provides a ginseng extract composition and a preparation method thereof, which can treat and prevent alcoholic fatty liver, and provides related applications.

[0005] The technical solution adopted by the present invention to solve its technical problem is: In a first aspect, the present application provides a method for preparing a ginseng extract composition, comprising the following steps: Extracting crude enzyme extract from fresh ginseng with fresh stems and leaves; Extract taproot total saponins from ginseng taproot; Extracting total saponins from ginseng root; Dissolving and mixing the enzyme-containing crude extract, the main root total saponins and the fibrous root total saponins with a buffer, and converting them at an enzyme suitable temperature for a preset time to obtain a crude production system; The water and water-soluble impurities in the crude product system are removed to obtain the ginseng extract composition.

[0006] The method for preparing the ginseng extract composition provided in the present application utilizes the enzyme activity in fresh ginseng to promote the conversion of saponins in the main root and fibrous root of ginseng. Experiments have confirmed that ginsenosides in specific parts are more conducive to the treatment and prevention of alcoholic fatty liver after conversion.

[0007] Furthermore, the step of dissolving and mixing the crude enzyme extract, the taproot total saponins and the fibrous root total saponins with a buffer solution comprises: The main root total saponins and the fibrous root total saponins are mixed to obtain a first mixture, and the first mixture is dissolved in the buffer solution which is 10 times the mass of the first mixture to obtain a first solution; Dissolving the crude enzyme extract with the buffer solution which is 10 times the mass of the crude enzyme extract to obtain a second solution; Mixing equal masses of the first solution and the second solution; The buffer solution is a phosphate buffer solution with a pH of 5.5 to 7.0.

[0008] This process can ensure that the enzyme activity is maintained and exerted, thereby more efficiently converting saponins, optimizing the effect of the enzymatic reaction, and ultimately improving the therapeutic effect of the ginseng extract composition.

[0009] Furthermore, in the first mixture, the mass ratio of the total saponins in the main root to the total saponins in the fibrous root is 2.6:1.

[0010] This specific mass ratio optimizes the ratio of taproot and fibrous root saponins, maximizing the effect of saponin conversion and contributing to the formation of richer active ingredients.

[0011] Furthermore, the step of extracting the enzyme-containing crude extract from fresh ginseng containing fresh stems and leaves comprises: The fresh ginseng is crushed into pulp at below 30°C, water is added and the mixture is centrifuged, the supernatants are combined, and the mixture is concentrated under reduced pressure at 30°C-40°C to obtain a concentrate, the concentrate is mixed with 95% edible alcohol in an amount that is 3 times the volume of the concentrate, and the insoluble matter is collected by centrifugation to obtain the enzyme-containing crude extract.

[0012] This process can protect the enzyme activity in fresh ginseng from being destroyed. Low temperature treatment and reduced pressure concentration effectively retain the biologically active components in fresh ginseng, especially the activity of enzymes, and make the obtained enzyme-containing crude extract purer, which helps to improve the efficiency of subsequent saponin conversion and the final quality of the extract.

[0013] Furthermore, the main root total saponins and the fibrous root total saponins are extracted according to the following steps: The roots of the corresponding parts are crushed, and extracted with 8 to 10 times the amount of water at 85°C to 95°C for at least 1 hour, and the extraction is repeated for multiple times. The extracts are combined, and the extracts are adsorbed with a macroporous adsorption resin, and the macroporous adsorption resin is rinsed with water to remove impurities, and then the macroporous adsorption resin is eluted with 70% to 90% edible alcohol. Finally, the eluate is concentrated and dried to obtain the saponins of the corresponding parts.

[0014] This process can effectively extract saponin components from ginseng, which is beneficial to improving the purity and activity of the extract and providing purer basic materials for subsequent enzymatic transformation.

[0015] Furthermore, the requirement for the conversion at the enzyme suitable temperature for a preset time is: keeping warm at 40° C. for 48 h to 96 h under stirring at 80 rpm to 120 rpm.

[0016] That is to say, the optimum temperature for the enzyme is 40°C, and the preset time is 48h~96h. This process can give full play to the catalytic effect of the enzyme in fresh ginseng, transform it into components with higher biological activity, and make the conversion efficiency of saponin higher. The obtained extract performs better in the treatment of alcoholic fatty liver.

[0017] Furthermore, the step of removing water and water-soluble impurities in the crude production system comprises: The crude product system is concentrated under reduced pressure to one tenth of the original volume to obtain a crude product concentrate, 95% edible alcohol having a volume three times that of the crude product concentrate is added to the crude product concentrate, and then the supernatant is evaporated to dryness to obtain a crude product; The crude product is dissolved in 40% to 50% edible alcohol and adsorbed on a macroporous adsorption resin. The macroporous adsorption resin is rinsed with water to remove impurities. The macroporous adsorption resin is then eluted with 85% to 95% edible alcohol. Finally, the eluate is concentrated and dried.

[0018] After purification and refining, the purity of the extract can be greatly improved, which is beneficial to improving the quality of the extract and ensuring its higher safety and stability in subsequent use, thereby improving its effectiveness in the treatment and prevention of alcoholic fatty liver.

[0019] In a second aspect, the present application provides a ginseng extract composition, which is prepared by the ginseng extract composition preparation method described in the first aspect and is particularly suitable for preventing and treating alcoholic fatty liver. After optimized extraction and conversion processes, the obtained composition has higher activity.

[0020] Furthermore, the total ginsenoside content of ginseng is ≥90wt%, containing ginsenosides S-Rg2, R-Rg2, S-Rg3, R-Rg3, Rg4, Rg5, Rg6, S-Rh1, R-Rh1, S-Rh2, R-Rh2, Rh3, Rh4, Rk1, Rk2, and Rk3.

[0021] In a third aspect, the present application provides an application of the ginseng extract composition as described in the second aspect, specifically mixed in a beverage or food.

[0022] The beneficial effects of the present invention are as follows: the present invention combines the enzymes in fresh ginseng containing fresh stems and leaves with the saponins in the main roots and fibrous roots, thereby promoting the transformation and extraction of ginsenosides; the natural enzymes in fresh ginseng help to transform the saponins into components with higher biological activity; the prepared ginseng extract composition can not only treat alcoholic fatty liver, but also has a preventive effect on alcoholic fatty liver. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a comparison chart of serum biochemical indexes in each prevention group.

[0024] Figure 2 It is a comparison chart of lipid metabolism indicators in liver tissue of each prevention group.

[0025] Figure 3 It is a comparison chart of lipid peroxidation indexes in liver tissue of each prevention group.

[0026] Figure 4 It is a comparison chart of body weight and liver weight changes in each prevention group.

[0027] Figure 5 Figure 2 is the liver anatomy of each prevention group.

[0028] Figure 6 It is the HE staining picture of liver tissues in each prevention group.

[0029] Figure 7 It is a comparison chart of serum biochemical indexes in each treatment group.

[0030] Figure 8 It is a comparison chart of lipid metabolism indicators in liver tissue of each treatment group.

[0031] Fig. 9 It is a comparison chart of lipid peroxidation indexes in liver tissue of each treatment group.

[0032] Fig.10 It is a comparison chart of body weight and liver weight changes in each treatment group.

[0033] Fig.11 are liver anatomy diagrams of each treatment group.

[0034] Fig.12 HE staining images of liver tissues in each treatment group. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the embodiments of the present disclosure clearer, the technical solution of the embodiments of the present disclosure is clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present disclosure, not all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] It should be understood that, under the premise of no conflict, any and all embodiments of the present invention can be combined with the technical features in any other embodiment or multiple other embodiments to obtain another embodiment. The present invention includes such a combination to obtain another embodiment.

[0037] Unless otherwise specified, all technical and scientific terms used herein have the standard meaning in the field to which the claimed subject matter belongs. If there are multiple definitions for a term, the definition herein shall prevail.

[0038] As a widely used and long-standing medicinal material, ginseng has the effect of sobering up. Studies have shown that ginsenosides have multiple therapeutic effects such as antioxidant, anti-inflammatory, vasodilation, anti-allergic, and anti-diabetic, and have no toxic side effects. Although ginseng is known to have multiple benefits and has the potential to treat alcoholic fatty liver, how to use the ingredients in ginseng to make it more beneficial and more focused on treating alcoholic fatty liver is a difficult problem.

[0039] Ginsenoside is a composite material extracted from ginseng. Its composition is complex, and the components extracted from different parts of ginseng are different. More than 40 kinds of ginsenosides have been separated from ginseng roots in the existing technology, and the composition of ginseng is still not fully explored.

[0040] The project team accidentally discovered that mixing the slurry of fresh ginseng containing fresh stems and leaves with saponins extracted from dried ginseng overnight and then recovering the saponins from the mixture is more effective in treating and preventing alcoholic fatty liver disease than directly extracting the whole root of ginseng. It is speculated that the enzyme contained in fresh ginseng can partially convert the saponins extracted from dried ginseng, and the converted mixture is more effective in treating and preventing alcoholic fatty liver disease.

[0041] Based on this, the project team continued to conduct experiments with different parts of ginseng under different conditions and adjusted a method for preparing a ginseng extract composition, including the following steps: S1: Extraction of crude enzyme extract from fresh ginseng with fresh stems and leaves.

[0042] S2: Extract taproot total saponins from ginseng taproot.

[0043] S3: Extract total saponins from ginseng root hairs.

[0044] S4: Dissolve and mix the crude enzyme extract, the main root total saponins and the fibrous root total saponins with a buffer solution, and transform them at a temperature suitable for the enzyme for a preset time to obtain a crude production system.

[0045] The water and water-soluble impurities in the crude product system are removed through purification in step S5 and refinement in step S6 to obtain a ginseng extract composition.

[0046] The prepared ginseng extract composition has a total ginsenoside content of ≥90wt%, and contains ginsenosides S-Rg2, R-Rg2, S-Rg3, R-Rg3, Rg4, Rg5, Rg6, S-Rh1, R-Rh1, S-Rh2, R-Rh2, Rh3, Rh4, Rk1, Rk2, and Rk3. It should be noted that the composition of ginsenosides is complex and has not been fully explored. It is also difficult to qualitatively and quantitatively measure the components of the ginseng extract composition after transformation. It is impossible to determine whether the benefit of the transformation in treating and preventing alcoholic fatty liver is due to the production of more unknown substances or the change in the specific saponin ratio. It only shows here that the ginseng extract composition prepared in this application can be measured to meet this condition, and it cannot be considered as a characterization of the effective ingredients (ingredients that are more conducive to the treatment and prevention of alcoholic fatty liver). The composition obtained by other extraction methods meets these conditions but does not guarantee that the effect equivalent to the composition prepared by the preparation method of this application can be obtained.

[0047] The ginseng extract composition can be used as general food, health care products, and medicines. For example, it can be made into medicinal wine, capsules, snacks, etc. Applying the ginseng extract composition to food or beverages is conducive to easily preventing or improving alcoholic fatty liver in daily life, increasing feasibility and popularity.

[0048] The ginseng extract composition in the following experiment was prepared by the following steps. For the convenience of description, the concentration of alcohol is assumed to be volume concentration.

[0049] Enzyme extraction: Take fresh ginseng with fresh stems and leaves and grind it into a homogenate at below 30℃, add 1-2 times the weight of water to mix, stir thoroughly and centrifuge, retain the supernatant, continue to mix it evenly with the precipitate with appropriate amount of water, centrifuge, combine the supernatants from multiple times, concentrate under reduced pressure at 30-40℃, mix the concentrate with 3 times 95% edible alcohol, centrifuge, and the obtained precipitate is the crude enzyme extract.

[0050] Extraction of total saponins from ginseng taproot: Crush the ginseng taproot, extract with 8-10 times the amount of water at 85-95°C for 1h, repeat the extraction 3 times, combine the extracts, adsorb on a macroporous adsorption resin column, wash away impurities with water, elute the saponins with 70%-90% alcohol, and concentrate and dry to obtain total saponins from ginseng taproot.

[0051] Extraction of total saponins from ginseng fibrous roots: extract total saponins according to the method of ginseng taproot.

[0052] Transformation: Mix the total saponins of ginseng taproot and ginseng fibrous root, add 10 times of phosphate buffer at pH 5.5-7.0 to fully dissolve them, dissolve the crude enzyme extract in 10 times of phosphate buffer at pH 5.5-7.0, and then mix with the saponin solution (first solution) in equal weight, and keep warm at 40°C for 48-96 hours with stirring at 80-120 rpm.

[0053] Purification: After the insulation is completed, the solution is concentrated under reduced pressure to one tenth of the volume, and then 3 times 95% edible alcohol is added to fully dissolve it. The supernatant is evaporated to dryness to obtain a crude product.

[0054] Refining: The crude product is dissolved in 40% to 50% edible alcohol and adsorbed on a macroporous adsorption resin column. After adsorption, water is used to wash away water-soluble impurities such as salt and sugar, and then eluted with 85% to 95% alcohol. The eluted solution is concentrated and dried to obtain a ginseng extract composition.

[0055] In addition, the ginseng extract control required for the control experiment was prepared as follows: Enzyme extraction: Take fresh ginseng roots without stems and leaves and grind them into a homogenate at below 30℃, add 1-2 times the weight of fresh ginseng water to mix, stir thoroughly and centrifuge, retain the supernatant, continue to mix the precipitate with appropriate amount of water, centrifuge, combine the supernatants from multiple times, concentrate under reduced pressure at 30-40℃, mix the concentrate with 3 times 95% edible alcohol, centrifuge, and the obtained precipitate is the crude enzyme extract.

[0056] Extraction of total saponins from ginseng roots: Crush the main root of ginseng, extract with 8-10 times the amount of water at 85-95°C for 1 hour, repeat the extraction three times, combine the extracts, adsorb on a macroporous adsorption resin column, wash away impurities with water, elute the saponins with 70%-90% alcohol, and concentrate and dry to obtain total saponins from the main root of ginseng.

[0057] Transformation: Add 10 times pH 5.5-7.0 phosphate buffer to fully dissolve the total saponins of ginseng taproot, dissolve the crude enzyme extract in 10 times pH 5.5-7.0 phosphate buffer, and then mix with the saponin solution (first solution) in equal weights, and keep warm at 40°C for 48-96 hours with stirring at 80-120 rpm.

[0058] Purification: After the insulation is completed, the solution is concentrated under reduced pressure to one tenth of the volume, and then 3 times 95% edible alcohol is added to fully dissolve it. The supernatant is evaporated to dryness to obtain a crude product.

[0059] Refining: Dissolve the crude product in 40%~50% edible alcohol and adsorb it on a macroporous adsorption resin column. After adsorption, wash away water-soluble impurities such as salt and sugar with water, then elute with 85%~95% alcohol, concentrate and dry the eluted solution to obtain the ginseng extract reference.

[0060] Experimental preparation Reagents: 0.9% saline, acetic acid, 4% paraformaldehyde, anhydrous ethanol, 95% ethanol, silybin, mouse alcohol liquid feed, mouse alcohol-free control liquid feed, maltodextrin, aspartate aminotransferase (AST / GOT) kit, alanine aminotransferase (ALT / GOT) kit, triglyceride (TG) kit, total cholesterol (TC) kit, reduced glutathione (GSH) ratio kit, malondialdehyde (MDA) kit and BCA protein concentration determination kit, etc.

[0061] To prepare the L-RGG solution, the ginseng extract composition prepared above was dissolved in a 20% ethanol solution to prepare a 20 mg / kg solution.

[0062] To prepare the H-RGG solution, the ginseng extract composition prepared above was dissolved in a 20% ethanol solution to prepare a 40 mg / kg solution.

[0063] To prepare the RT solution, dissolve the ginseng extract control prepared above in 20% ethanol solution to make a 40 mg / kg solution.

[0064] Instruments: constant temperature water bath, ELISA reader, high-speed low-temperature centrifuge, homogenizer, etc.

[0065] Experimental mice: healthy SPF grade C57BL / 6 J male mice (weight 20-22g) Statistical analysis of experimental data was performed using GraphPad Prism 10.0 software, and the final data were expressed as mean ± standard deviation. One-way analysis of variance was used to compare differences between groups. Statistical significance was defined as follows: * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; **** indicates p < 0.0001.

[0066] Experimental Procedure 1. After five days of adaptive feeding, the mice in the blank prevention group were fed with alcohol-free control liquid feed, and from the first day of feeding with alcohol-free control liquid feed, they were gavaged with 0.1 ml of normal saline every day. After five days of adaptive feeding, the mice in the blank treatment group were fed with alcohol-free control liquid feed, and on the tenth day of feeding with alcohol-free control liquid feed, they were gavaged with 0.1 ml of normal saline.

[0067] 2. After five days of adaptive feeding, the mice in the model prevention group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations (1% on the first day, 2% on the second day, 3% on the third day, 4% on the fourth day, and 5% on the fifth day), and then continued to be fed with 5% (vol / vol) alcohol liquid feed. From the first day of feeding with alcohol liquid feed, 0.1ml of normal saline was gavaged every day. After five days of adaptive feeding, the mice in the model treatment group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations, and then continued to be fed with 5% (vol / vol) alcohol liquid feed. On the sixth day of feeding with 5% alcohol liquid feed (equivalent to the tenth day of feeding with alcohol liquid feed), 0.1ml of normal saline was gavaged.

[0068] 3. After five days of adaptive feeding, mice in the silybin prevention group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations, and then continued to be fed with 5% (vol / vol) alcohol liquid feed. From the first day of feeding with alcohol liquid feed, 0.1ml of silybin solution (50mg / kg) was gavaged every day. After five days of adaptive feeding, mice in the silybin treatment group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations, and then continued to be fed with 5% (vol / vol) alcohol liquid feed. On the sixth day of feeding with 5% alcohol liquid feed, 0.1ml of silybin solution (50mg / kg) was gavaged.

[0069] 4. After five days of adaptive feeding, the mice in the L-RGG prevention group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations. After the mice adapted to the alcohol concentration, they continued to be fed with 5% (vol / vol) alcohol liquid feed. From the first day of feeding with alcohol liquid feed, they were gavaged with 0.1ml L-RGG solution (20mg / kg) every day. After five days of adaptive feeding, the mice in the L-RGG treatment group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations. After the mice adapted to the alcohol concentration, they continued to be fed with 5% (vol / vol) alcohol liquid feed. On the sixth day of feeding with 5% alcohol liquid feed, they were gavaged with 0.1ml L-RGG solution (20mg / kg).

[0070] 5. After five days of adaptive feeding, the mice in the H-RGG prevention group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations. After the mice adapted to the alcohol concentration, they continued to be fed with 5% (vol / vol) alcohol liquid feed. From the first day of feeding with alcohol liquid feed, they were gavaged with 0.1ml H-RGG solution (40mg / kg) every day. After five days of adaptive feeding, the mice in the H-RGG treatment group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations. After the mice adapted to the alcohol concentration, they continued to be fed with 5% (vol / vol) alcohol liquid feed. On the sixth day of feeding with 5% alcohol liquid feed, they were gavaged with 0.1ml H-RGG solution (20mg / kg).

[0071] 6. After five days of adaptive feeding, mice in the RT prevention group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations, and then continued to be fed with 5% (vol / vol) alcohol liquid feed. From the first day of feeding with alcohol liquid feed, 0.1ml of ginsenoside solution (40mg / kg) was gavaged every day. After five days of adaptive feeding, mice in the RT treatment group were fed with 1%~5% (vol / vol) alcohol liquid feed at increasing concentrations, and then continued to be fed with 5% (vol / vol) alcohol liquid feed. On the sixth day of feeding with 5% alcohol liquid feed, 0.1ml of ginsenoside solution (40mg / kg) was gavaged.

[0072] 7. On the eleventh day of the experiment (the sixteenth day if adaptive feeding is included), all mice were gavaged with 95% ethanol (5 g / kg), and the mice in each group died suddenly 9 hours after gavage.

[0073] 8. Collect blood by removing the eyeball. Immediately after removing the eyeball and collecting blood, remove the entire liver and rinse it several times in pre-cooled saline to clean the residual blood in the liver for weighing. Take the largest right lobe of each liver and immerse it in 4% paraformaldehyde for the preparation of tissue sections for histopathological evaluation. The remaining liver tissue is prepared into the corresponding liver homogenate for testing according to the corresponding kit instructions.

[0074] The experimental results are as follows Figures 1 to 12 In the figure, the blank group is represented by Control, the model group is represented by Model, the silybin group is represented by Silybin, the L-RGG group is represented by L-RGG, the H-RGG group is represented by H-RGG, and the ginseng whole extract group is represented by RT.

[0075] Reference Figures 1 to 6The experimental results of each prevention group showed that the 40 mg / kg RGG dose can effectively inhibit the increase of triglyceride (TG), total cholesterol (TC), malondialdehyde (MDA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in the liver of mice; effectively promote the synthesis of glutathione (GSH); curb the decrease in mouse body weight and the weight gain of the liver; HE staining results showed that RGG significantly reduced the formation of liver fat particles; it has a good preventive effect on alcoholic liver disease, but it does not have a dose-dependent relationship. The preventive effect of the control ginseng extract composition 2 was not obvious, and no statistically significant effect was found, indicating that the crude enzyme in the fresh ginseng root alone cannot be converted into effective ingredients.

[0076] Reference Figures 7 to 12 The experimental results of each treatment group showed that high-dose RGG (ginseng extract composition of the present application) can effectively curb the increase of triglyceride (TG), total cholesterol (TC), malondialdehyde (MDA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in mouse liver; effectively promote the synthesis of glutathione (GSH); curb the decrease in mouse body weight and the weight gain of liver; HE staining results show that RGG significantly reduces the formation of liver fat particles; has a good therapeutic effect on alcoholic liver disease, and shows a dose-dependent relationship. The therapeutic effect of the ginseng extract control group was not obvious, and no statistically significant effect was found, indicating that the crude enzyme in fresh ginseng root alone cannot be converted into effective ingredients.

[0077] In the description of this specification, the description with reference to the terms "one embodiment", "certain embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0078] The above is a preferred embodiment of the present invention. It should be pointed out that a person skilled in the art can make several improvements and modifications without departing from the principle of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A method for preparing a ginseng extract composition, characterized in that: The following steps are involved: Extracting crude enzyme extract from fresh ginseng with fresh stems and leaves; Extract taproot total saponins from ginseng taproot; Extracting total saponins from ginseng root; Dissolving and mixing the enzyme-containing crude extract, the main root total saponins and the fibrous root total saponins with a buffer solution, and converting them at an enzyme suitable temperature for a preset time to obtain a crude production system; The water and water-soluble impurities in the crude product system are removed to obtain the ginseng extract composition.

2. The method for preparing the ginseng extract composition according to claim 1, characterized in that: The step of dissolving and mixing the crude enzyme extract, the taproot total saponins and the fibrous root total saponins with a buffer comprises: The main root total saponins and the fibrous root total saponins are mixed to obtain a first mixture, and the first mixture is dissolved in the buffer solution which is 10 times the mass of the first mixture to obtain a first solution; Dissolving the crude enzyme extract with the buffer solution which is 10 times the mass of the crude enzyme extract to obtain a second solution; Mixing equal masses of the first solution and the second solution; The buffer solution is a phosphate buffer solution with a pH of 5.5 to 7.

0.

3. The method for preparing the ginseng extract composition according to claim 2, characterized in that: In the first mixture, the mass ratio of the total saponins in the main root to the total saponins in the fibrous root is 2.6:

1.

4. The method for preparing the ginseng extract composition according to claim 1 or 2, characterized in that: The step of extracting the enzyme-containing crude extract from fresh ginseng containing fresh stems and leaves comprises: The fresh ginseng containing fresh stems and leaves is crushed into pulp at below 30°C, water is added and the mixture is centrifuged, the supernatants are combined, and the mixture is concentrated under reduced pressure at 30°C-40°C to obtain a concentrate, the concentrate is mixed with 95% edible alcohol in an amount that is 3 times the volume of the concentrate, and the insoluble matter is collected by centrifugation to obtain the enzyme-containing crude extract.

5. The method for preparing the ginseng extract composition according to claim 1 or 2, characterized in that: The main root total saponins and the fibrous root total saponins are extracted according to the following steps: The roots of the corresponding parts are crushed, and extracted with 8 to 10 times the amount of water at 85°C to 95°C for at least 1 hour, and the extraction is repeated for multiple times. The extracts are combined, and the extracts are adsorbed with a macroporous adsorption resin, and the macroporous adsorption resin is rinsed with water to remove impurities, and then the macroporous adsorption resin is eluted with 70% to 90% edible alcohol. Finally, the eluate is concentrated and dried to obtain the saponins of the corresponding parts.

6. The method for preparing the ginseng extract composition according to claim 1, characterized in that: The requirement for the conversion at the enzyme suitable temperature for a preset time is: keeping warm at 40° C. for 48 h to 96 h under stirring at 80 rpm to 120 rpm.

7. The method for preparing the ginseng extract composition according to claim 1, characterized in that: The step of removing water and water-soluble impurities in the crude product system comprises: The crude product system is concentrated under reduced pressure to one tenth of the original volume to obtain a crude product concentrate, 95% edible alcohol having a volume three times that of the crude product concentrate is added to the crude product concentrate, and then the supernatant is evaporated to dryness to obtain a crude product; The crude product is dissolved in 40% to 50% edible alcohol and adsorbed on a macroporous adsorption resin. The macroporous adsorption resin is rinsed with water to remove impurities. The macroporous adsorption resin is then eluted with 85% to 95% edible alcohol. Finally, the eluate is concentrated and dried.

8. A ginseng extract composition, characterized in that: The ginseng extract composition is prepared by the preparation method of any one of claims 1 to 7.

9. The ginseng extract composition according to claim 8, characterized in that: The total ginsenoside content of ginseng is ≥90wt%, containing ginsenosides S-Rg2, R-Rg2, S-Rg3, R-Rg3, Rg4, Rg5, Rg6, S-Rh1, R-Rh1, S-Rh2, R-Rh2, Rh3, Rh4, Rk1, Rk2, and Rk3.

10. A use of the ginseng extract composition according to any one of claims 8 or 9, characterized in that: Mix in beverages or food.

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