Plant polysaccharide hydrogel medical dressing for promoting tissue damage repair and application of plant polysaccharide hydrogel medical dressing

Abstract

The invention belongs to the technical field of regenerated medical dressings, particularly relates to a plant polysaccharide hydrogel medical dressing for promoting tissue damage repair and application thereof, and provides the following scheme that a medical and mechanical combined product comprises a regenerated medical dressing and a low-temperature sterile storage box for storing the regenerated medical dressing, the regenerated medical dressing comprises a hydrogel main body, open pores formed in the hydrogel main body, sustained-release vesicles arranged in the open pores, and stem cells and a cell culture medium which are filled in the sustained-release vesicles. The stem cell hydrogel can rapidly break blood, rapidly stop bleeding by being attached to a wound surface and promote wound closing, stem cells are inoculated to the upper surface of the hydrogel formed by dendrobium polysaccharides, aloe, lily, coptis chinensis and honeysuckle, safe application of the stem cells is achieved, active factors secreted by the stem cells are slowly released to the skin wound surface, and the wound healing effect is achieved. Through contact stimulation between cells, various cytokines can be secreted better, and the skin repair efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of regenerative medical dressings, in particular to a plant polysaccharide hydrogel medical dressing for promoting tissue damage repair and application thereof. Background Art

[0002] Burns are injuries caused by flames, high-temperature solids, and strong radiant heat. Burns are tissue damage caused by high temperatures, chemicals, or electricity. The extent of burns varies depending on the temperature and duration of action. They mainly affect the skin and mucous membranes, and in severe cases, may also damage subcutaneous and submucosal tissues.

[0003] Skin burns can lead to infection, water and electrolyte imbalance, shock, and local scars. After a burn, the integrity of the skin will be damaged to a certain extent, which may cause bacteria to enter the body, causing bacterial infection, pus discharge from the skin, pain and other adverse symptoms. After a large area of ​​burns, a large amount of fluid in the body will be lost, causing water and electrolyte imbalance, accompanied by fatigue and weakness in the limbs. After a burn, the integrity of the skin will be damaged, causing a large amount of fluid to evaporate, and most patients will experience hypovolemic shock. In addition, bacterial infection may be accompanied by septic shock. After a patient is partially burned, if protective measures are not in place, scar hyperplasia will occur on the skin, and obvious scars will appear.

[0004] Skin wound dressings can cover wounds and provide a temporary barrier to resist external infection, and serve as an inductive template to guide skin cell reorganization and subsequent host tissue penetration and integration, which has a significant effect on wound healing.

[0005] Mesencjymal stem cells and various bioactive factors secreted by stem cells and extracellular carriers (including exosomes and microvesicles, collectively referred to as exosomes) can promote cell proliferation, angiogenesis, collagen synthesis and repair of skin barrier function, thereby accelerating skin wound healing. Encapsulating MSCs or EXOs with polyoxyethylene, polyoxypropylene, and ether hydrogels to cover the surface of damaged mouse skin can promote the synthesis of collagen I and collagen III in damaged tissues, reduce the secretion of IL-6, TNF-α, CD68, and CD206, and greatly promote skin wound healing and re-epithelialization.

[0006] Therefore, it is of great significance to further strengthen the hydrogel's ability to promote burn wound healing, antibacterial and anti-infection. Summary of the invention

[0007] Based on the technical problems raised by the background technology, the present invention proposes a plant polysaccharide hydrogel medical dressing for promoting tissue damage repair and its application.

[0008] The plant polysaccharide hydrogel medical dressing for promoting tissue damage repair proposed by the present invention comprises a hydrogel body, an opening formed on the hydrogel body, a sustained-release vesicle disposed in the opening, and stem cells and a cell culture medium filled in the sustained-release vesicle;

[0009] An isolation membrane is disposed at the bottom of the hydrogel body, and a porous polymer material film outer layer is disposed at the top of the hydrogel body;

[0010] The hydrogel body comprises the following components in percentage by mass: 5%-15% of superfine dendrobium polysaccharide powder, 5%-8% of aloe extract, 2%-5% of lily extract, 1%-3% of coptis extract, 2%-5% of honeysuckle extract, 3%-5% of xanthan gum, 1%-3% of glycerol, 0.5%-1% of inorganic base, 0.5%-1% of inorganic salt, and the balance is water.

[0011] Preferably, the raw material for extracting the ultrafine Dendrobium powder is preferably Dendrobium officinale.

[0012] Preferably, the inorganic base is a mixture of one or more of sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide.

[0013] Preferably, the outer layer of the polymer material film is made of one or a mixture of polylactic acid, polyglycolic acid, lactic acid-glycolic acid copolymer, and polycaprolactone.

[0014] Preferably, the water holding capacity of the stem cells and the cell culture medium is %.

[0015] Preferably, the sustained-release vesicle consists of a polyvinyl alcohol layer and a deacetylated chitosan layer, wherein the deacetylated chitosan layer is located inside the polyvinyl alcohol layer, and the stem cells and cell culture medium are filled in the deacetylated chitosan layer.

[0016] The method for preparing the hydrogel body comprises the following steps:

[0017] A1: Preparation of Dendrobium officinale extract powder: Take the stem of Dendrobium officinale, crush it, add 80% ethanol solution by volume, extract it in a water bath at 55℃ for 1.5h, centrifuge it at 5000r / min, collect the alcohol extract and alcohol precipitate of Dendrobium officinale respectively, concentrate the alcohol extract under reduced pressure, freeze-dry it to obtain powdered extract 1; dry the alcohol precipitate of Dendrobium officinale to remove residual ethanol, add distilled water, extract it at 100℃ for 1.5h, centrifuge it at 5000r / min, collect the water extract, freeze-dry it after rotary evaporation, and obtain powdered extract 2; mix Dendrobium officinale extract 1 and 2 to prepare Dendrobium officinale extract powder, the extractant ratio is: the ratio of 80% ethanol solution to Dendrobium officinale is 1g:20mL; the ratio of water to Dendrobium officinale alcohol precipitate is 1g:10mL;

[0018] A2: Gel preparation: Add the prepared Dendrobium officinale extract powder into pure water preheated to 40-80°C, stir at a uniform speed, add aloe vera, lily, coptis root, honeysuckle extract, xanthan gum and glycerin, stir at a uniform speed for 10-20 minutes, then mix the prepared chitosan sol with sodium alginate solution in a volume ratio of 1:1, and stir and mix with the aid of high-frequency oscillation to ensure uniform dispersion of the components;

[0019] A3: Cross-linking to form gel: After mixing and stirring for 30 minutes, an inorganic base is added to adjust the pH of the mixed gel solution to about 7.0, and inorganic salts and multivalent cationic cross-linking agent CaCl2 are added for cross-linking to form a stable hydrogel network structure.

[0020] The extraction method of the aloe extract, lily extract, coptis extract and honeysuckle extract comprises the following steps:

[0021] B1: crushing, drying aloe, lily, coptis root or honeysuckle respectively, crushing into 100-200 mesh by a crusher, and collecting them separately for standby use;

[0022] B2: Extraction, soaking aloe vera powder, lily powder, coptis root powder or honeysuckle powder for 5-10 hours, heating at 50°C-80°C, centrifuging at 3000R / min for 1h-2h, taking the centrifuge liquid, and drying it to obtain aloe vera extract, lily extract, coptis root extract and honeysuckle extract respectively.

[0023] Preferably, the method for preparing the chitosan sol and sodium alginate solution comprises the following steps:

[0024] C1: Preparation of chitosan sol: Chitosan was dissolved in 0.25 mol / L acetic acid to prepare a solution with a mass concentration of 30 g / L, and its acidity was neutralized with 0.1 mol / L NaOH solution until the pH value reached about 6.3 to form chitosan sol;

[0025] C2: preparing a sodium alginate solution: dissolving sodium alginate in deionized water to form a sodium alginate aqueous solution with a mass concentration of 30 g / L.

[0026] The present invention also proposes an application of a plant polysaccharide hydrogel medical dressing that promotes tissue damage repair, including the regenerative medical dressing and a low-temperature sterile storage box for storing the regenerative medical dressing, wherein a regenerative dressing placement slot is provided in the middle of the low-temperature sterile storage box, and material extraction recesses are provided on both sides of the regenerative dressing placement slot, a refrigeration component and a power supply are provided in the low-temperature sterile storage box, and the temperature in the low-temperature sterile storage box is maintained at 1°C-5°C.

[0027] The beneficial effects of the present invention are:

[0028] 1. Inoculate stem cells on the upper surface of the hydrogel formed by dendrobium polysaccharide and aloe, lily, coptis root and honeysuckle to isolate the stem cells from the wound. The active factors secreted by the stem cells can penetrate into the wound through the hydrogel dressing, thereby promoting wound healing while avoiding direct contact between the stem cells and the wound, achieving safe application of stem cells. The active factors secreted by the stem cells are slowly released into the skin wound. Through the contact stimulation between cells, various cytokines can be better secreted, thereby improving the efficiency of skin repair.

[0029] 2. The combination of chitosan sol and sodium alginate solution can quickly cut off blood flow, adhere to the wound surface, quickly stop bleeding, and promote wound closure.

[0030] 3. Dendrobium officinale: The hydrogel formed by using Dendrobium officinale has good water holding rate and gel strength, and is biocompatible and biodegradable;

[0031] 4. Aloe vera: It has the effects of softening and purging, clearing away heat and dampness. It acts on the local area of ​​scald, has the effects of detoxification, clearing heat and relieving pain. Secondly, aloe vera is helpful in removing scars.

[0032] 5. Lily: Lily is rich in vitamins and trace elements, which are beneficial to skin metabolism, and have the effects of beautifying and removing scars.

[0033] 6. Coptis chinensis: It has many uses, such as clearing away heat, purging fire, drying dampness, detoxifying, clearing the heart and relieving restlessness. Berberine is used to treat scalds and burns. Clinical use has shown that using berberine to treat burn wounds can not only effectively prevent infection, shorten the course of treatment, but also relieve wound pain. Patients feel cool and comfortable. Except for wounds that have been severely infected before medication, second-degree burns can reduce scars.

[0034] 7. Honeysuckle can promote wound healing. Honeysuckle is cold in nature and has the effects of clearing away heat and detoxifying, antibacterial and anti-inflammatory. It is beneficial to enhance the body's immune ability and promote the healing of burn wounds. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of the structure of the plant polysaccharide hydrogel medical dressing for promoting tissue damage repair proposed by the present invention;

[0036] Figure 2 This is a schematic diagram of a partial structure enlargement of the plant polysaccharide hydrogel medical dressing for promoting tissue damage repair proposed by the present invention;

[0037] Figure 3 This is a schematic diagram of the application product structure of the plant polysaccharide hydrogel medical dressing for promoting tissue damage repair proposed by the present invention.

[0038] In the figure: 1. Hydrogel body; 2. Sustained-release vesicles; 2-1. Polyvinyl alcohol layer; 2-2. Deacetylated chitosan layer; 3. Cell culture medium; 4. Stem cells; 5. Outer layer of porous polymer film; 6. Isolation membrane; 7. Low-temperature sterile storage box; 8. Regenerative dressing placement slot; 9. Material removal recess. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0040] Example 1, reference Figure 1-3 , plant polysaccharide hydrogel medical dressing for promoting tissue damage repair and its application, the drug-device combination product includes a regenerative medical dressing and a low-temperature sterile storage box 7 for storing the regenerative medical dressing, a regenerative dressing placement groove 8 is opened in the middle of the low-temperature sterile storage box 7, and material extraction recesses 9 are opened on both sides of the regenerative dressing placement groove 8.

[0041] A refrigeration component and a power supply are provided in the low-temperature sterile storage box 7, and the temperature in the low-temperature sterile storage box 7 is maintained at 1°C-5°C;

[0042] The regenerative medical dressing comprises a hydrogel body 1, an opening formed on the hydrogel body 1, a sustained-release vesicle 2 disposed in the opening, and stem cells 4 and a cell culture medium 3 filled in the sustained-release vesicle 2;

[0043] An isolation membrane 6 is provided at the bottom of the hydrogel body 1, and a porous polymer material film outer layer 5 is provided at the top of the hydrogel body 1;

[0044] The hydrogel body 1 includes the following components in percentage by mass: 8% of superfine dendrobium polysaccharide powder, 5% of aloe extract, 2% of lily extract, 1% of coptis extract, 2% of honeysuckle extract, 3% of xanthan gum, 3% of glycerol, 0.5% of inorganic base, 0.5% of inorganic salt, and the balance is water.

[0045] The raw material for extracting the ultrafine powder of Dendrobium is preferably Dendrobium officinale.

[0046] The inorganic base is a mixture of one or more of sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide.

[0047] The outer layer 5 of the polymer film is made of one or a mixture of polylactic acid, polyglycolic acid, lactic acid-glycolic acid copolymer, and polycaprolactone.

[0048] The water holding capacity of the stem cells 4 and the cell culture medium 3 is 30%-50%.

[0049] The sustained-release vesicle 2 is composed of a polyvinyl alcohol layer 2-1 and a deacetylated chitosan layer 2-2, wherein the deacetylated chitosan layer 2-2 is located inside the polyvinyl alcohol layer 2-1, and the stem cells 4 and the cell culture medium 3 are filled in the deacetylated chitosan layer 2-2.

[0050] The method for preparing the hydrogel body 1 comprises the following steps:

[0051] A1: Preparation of Dendrobium officinale extract powder: Take the stem of Dendrobium officinale, crush it, add 80% ethanol solution by volume, extract it in a water bath at 55℃ for 1.5h, centrifuge it at 5000r / min, collect the alcohol extract and alcohol precipitate of Dendrobium officinale respectively, concentrate the alcohol extract under reduced pressure, freeze-dry it to obtain powdered extract 1; dry the alcohol precipitate of Dendrobium officinale to remove residual ethanol, add distilled water, extract it at 100℃ for 1.5h, centrifuge it at 5000r / min, collect the water extract, freeze-dry it after rotary evaporation, and obtain powdered extract 2; mix Dendrobium officinale extract 1 and 2 to prepare Dendrobium officinale extract powder, the extractant ratio is: the ratio of 80% ethanol solution to Dendrobium officinale is 1g:20mL; the ratio of water to Dendrobium officinale alcohol precipitate is 1g:10mL;

[0052] A2: Gel preparation: Add the prepared Dendrobium officinale extract powder into pure water preheated to 40-80°C, stir at a uniform speed, add aloe vera, lily, coptis root, honeysuckle extract, xanthan gum and glycerin, stir at a uniform speed for 10-20 minutes, then mix the prepared chitosan sol with sodium alginate solution in a volume ratio of 1:1, and stir and mix with the aid of high-frequency oscillation to ensure uniform dispersion of the components;

[0053] A3: Cross-linking to form gel: After mixing and stirring for 30 minutes, an inorganic base is added to adjust the pH of the mixed gel solution to about 7.0, and inorganic salts and multivalent cationic cross-linking agent CaCl2 are added for cross-linking to form a stable hydrogel network structure.

[0054] The extraction method of aloe extract, lily extract, coptis extract and honeysuckle extract comprises the following steps:

[0055] B1: crushing, drying aloe, lily, coptis root or honeysuckle respectively, crushing into 100-200 mesh by a crusher, and collecting them separately for standby use;

[0056] B2: Extraction, soaking aloe vera powder, lily powder, coptis root powder or honeysuckle powder for 5-10 hours, heating at 50°C-80°C, centrifuging at 3000R / min for 1h-2h, taking the centrifuge liquid, and drying it to obtain aloe vera extract, lily extract, coptis root extract and honeysuckle extract respectively.

[0057] The method for preparing chitosan sol and sodium alginate solution comprises the following steps:

[0058] C1: Preparation of chitosan sol: Chitosan was dissolved in 0.25 mol / L acetic acid to prepare a solution with a mass concentration of 30 g / L, and its acidity was neutralized with 0.1 mol / L NaOH solution until the pH value reached about 6.3 to form chitosan sol;

[0059] C2: preparing a sodium alginate solution: dissolving sodium alginate in deionized water to form a sodium alginate aqueous solution with a mass concentration of 30 g / L.

[0060] Example 2, 8% of superfine dendrobium polysaccharide powder, 6% of aloe extract, 3% of lily extract, 3% of coptis extract, 3% of honeysuckle extract, 3% of xanthan gum, 2% of glycerol, 0.5% of inorganic base, 0.5% of inorganic salt, and the balance is water.

[0061] Example 3, 10% of superfine dendrobium polysaccharide powder, 7% of aloe extract, 5% of lily extract, 3% of coptis extract, 3% of honeysuckle extract, 3% of xanthan gum, 2% of glycerol, 0.5% of inorganic base, 0.5% of inorganic salt, and the balance is water.

[0062] Example 4, 12% of superfine dendrobium polysaccharide powder, 5% of aloe extract, 5% of lily extract, 3% of coptis extract, 5% of honeysuckle extract, 3% of xanthan gum, 2% of glycerol, 0.8% of inorganic base, 0.8% of inorganic salt, and the balance is water.

[0063] Example 5, 15% of superfine dendrobium polysaccharide powder, 5% of aloe extract, 5% of lily extract, 3% of coptis extract, 5% of honeysuckle extract, 2% of xanthan gum, 2% of glycerol, 1% of inorganic base, 1% of inorganic salt, and the balance is water.

[0064] Mouse experiments:

[0065] The hydrogels were prepared by using the above Examples 1-5 respectively, and the dressing test was carried out on the burned mouse skin, and the following table was obtained:

[0066]

[0067]

[0068] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A plant polysaccharide hydrogel medical dressing for promoting tissue damage repair, characterized in that: The regenerative medical dressing comprises a hydrogel body (1), an opening formed on the hydrogel body (1), a sustained-release vesicle (2) disposed in the opening, and stem cells (4) and a cell culture medium (3) filled in the sustained-release vesicle (2); An isolation membrane (6) is provided at the bottom of the hydrogel body (1), and a porous polymer material film outer layer (5) is provided at the top of the hydrogel body (1); The hydrogel body (1) comprises the following components in percentage by mass: 5%-15% of superfine dendrobium powder, 5%-8% of aloe extract, 2%-5% of lily extract, 1%-3% of coptis extract, 2%-5% of honeysuckle extract, 3%-5% of xanthan gum, 1%-3% of glycerol, 0.5%-1% of inorganic base, 0.5%-1% of inorganic salt, and the balance is water.

2. The plant polysaccharide hydrogel medical dressing for promoting tissue damage repair according to claim 1, characterized in that: The raw material for extracting the ultrafine Dendrobium powder is preferably Dendrobium officinale.

3. The plant polysaccharide hydrogel medical dressing for promoting tissue damage repair according to claim 1, characterized in that: The inorganic base is a mixture of one or more of sodium carbonate, potassium carbonate, sodium hydroxide and potassium hydroxide.

4. The plant polysaccharide hydrogel medical dressing for promoting tissue damage repair according to claim 1, characterized in that: The polymer material film outer layer (5) is made of one or a mixture of polylactic acid, polyglycolic acid, lactic acid-glycolic acid copolymer, and polycaprolactone.

5. The plant polysaccharide hydrogel medical dressing for promoting tissue damage repair and its application according to claim 1, characterized in that: The water holding capacity of the stem cells (4) and the cell culture medium (3) is 30%-50%.

6. The plant polysaccharide hydrogel medical dressing for promoting tissue damage repair according to claim 1, characterized in that: The sustained-release vesicle (2) is composed of a polyvinyl alcohol layer (2-1) and a deacetylated chitosan layer (2-2), wherein the deacetylated chitosan layer (2-2) is located inside the polyvinyl alcohol layer (2-1), and the stem cells (4) and the cell culture medium (3) are filled in the deacetylated chitosan layer (2-2).

7. The plant polysaccharide hydrogel medical dressing for promoting tissue damage repair according to claim 1, characterized in that: The method for preparing the hydrogel body (1) comprises the following steps: A1 Preparation of Dendrobium Officinale Extract Powder: Take the stem of Dendrobium Officinale, crush it, add 80% ethanol solution by volume, extract it in a water bath at 55℃ for 1.5h, centrifuge it at 5000r / min, collect the alcohol extract and alcohol precipitate of Dendrobium Officinale respectively, concentrate the alcohol extract under reduced pressure, freeze-dry it to obtain powdered extract 1; dry the alcohol precipitate of Dendrobium Officinale to remove residual ethanol, add distilled water, extract it at 100℃ for 1.5h, centrifuge it at 5000r / min, collect the water extract, freeze-dry it after rotary evaporation, and obtain powdered extract 2; mix Dendrobium Officinale Extract 1 and 2 to prepare Dendrobium Officinale Extract Powder, the extractant ratio is: the ratio of 80% ethanol solution to Dendrobium is 1g:20mL; the ratio of water to Dendrobium alcohol precipitate is 1g:10mL; Preparation of A2 gel: Add the prepared Dendrobium officinale extract powder into pure water preheated to 40-80°C and stir at a uniform speed, then add aloe vera, lily, coptis root, honeysuckle extract, xanthan gum and glycerin, stir at a uniform speed for 10-20 minutes, then mix the prepared chitosan sol and sodium alginate solution in a volume ratio of 1:1, and stir and mix by high-frequency oscillation to ensure uniform dispersion of the components; A3 cross-linking to form a gel: After mixing and stirring for 30 minutes, an inorganic base is added to adjust the pH of the mixed gel solution to about 7.0, and an inorganic salt and a multivalent cationic cross-linking agent CaCl2 are added for cross-linking to form a stable hydrogel network structure.

8. The plant polysaccharide hydrogel medical dressing for promoting tissue damage repair according to claim 1, characterized in that: The extraction method of the aloe extract, lily extract, coptis extract and honeysuckle extract comprises the following steps: B1: crushing, drying aloe, lily, coptis root or honeysuckle respectively, crushing into 100-200 mesh by a crusher, and collecting them separately for standby use; B2: Extraction, soaking aloe vera powder, lily powder, coptis root powder or honeysuckle powder for 5-10 hours, heating at 50°C-80°C, centrifuging at 3000R / min for 1h-2h, taking the centrifuge liquid, and drying it to obtain aloe vera extract, lily extract, coptis root extract and honeysuckle extract respectively.

9. The plant polysaccharide hydrogel medical dressing for promoting tissue damage repair according to claim 7, characterized in that: The method for preparing chitosan sol and sodium alginate solution comprises the following steps: C1: Preparation of chitosan sol: Chitosan was dissolved in 0.25 mol / L acetic acid to prepare a solution with a mass concentration of 30 g / L, and its acidity was neutralized with 0.1 mol / L NaOH solution until the pH value reached about 6.3 to form chitosan sol; C2: preparing a sodium alginate solution: dissolving sodium alginate in deionized water to form a sodium alginate aqueous solution with a mass concentration of 30 g / L.

10. The use of the plant polysaccharide hydrogel medical dressing for promoting tissue damage repair according to claim 1, characterized in that: The invention comprises the regenerative medical dressing and a low-temperature sterile storage box (7) for storing the regenerative medical dressing, wherein a regenerative dressing placement slot (8) is provided in the middle of the low-temperature sterile storage box (7), and material extraction recesses (9) are provided on both sides of the regenerative dressing placement slot (8), a refrigeration component and a power supply are provided in the low-temperature sterile storage box (7), and the temperature in the low-temperature sterile storage box (7) is maintained at 1°C-5°C.

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