Enzyme immunoassay cleaning system, automatic enzyme immunoassay detection device and reaction container cleaning method

Abstract

The invention discloses an enzyme-free cleaning system, an automatic enzyme-free detection device and a reaction container cleaning method.The enzyme-free cleaning system comprises a flushing barrel and a plurality of cleaning barrels, a filling pump can be communicated with the flushing barrel or one of the cleaning barrels through a control valve set, and a cleaning head comprises a liquid injection needle and a liquid suction needle; the filling pump can further be in fluid communication with a liquid injection needle of the cleaning head, the cleaning head can be matched with an enzyme immunoassay reaction container cleaning position of the cleaning tank, when the filling pump is communicated with one cleaning barrel, the filling pump drives cleaning liquid to be injected into an enzyme immunoassay reaction container in the cleaning position through the liquid injection needle, and a liquid suction needle of the cleaning head can further be in fluid communication with a waste liquid collecting tank. The length of the liquid suction needle is larger than that of the liquid injection needle, the cleaning tank further comprises a suction opening, the suction opening can also be in fluid communication with the waste liquid collecting tank, the waste liquid collecting tank is further connected with a vacuum pump, the vacuum pump can suck the waste liquid collecting tank, the waste liquid collecting tank is in a negative pressure state, and the enzyme no-clean system remarkably improves the cleaning effect.

Description

Technical Field

[0001] The invention belongs to the technical field of automated enzyme-free assay and related equipment, and in particular relates to an enzyme-free cleaning system, an automated enzyme-free assay device and a reaction container cleaning method. Background Art

[0002] In vitro diagnosis is a relatively complex interdisciplinary subject. In the biomedical field, it mainly relies on the development of biochemistry, genetics, molecular biology, molecular imaging, cytology, immunology, microbiology and other disciplines. The development of in vitro diagnosis is inseparable from the coordinated development of various interdisciplinary subjects. In recent years, immunology, as an important branch that developed relatively early, has experienced a development process from the monopoly of large foreign companies or head companies to the rise of domestic equipment, forming a good situation in which domestic manufacturers have gradually developed mature immunodiagnostic platforms of various types. Automated enzyme-linked immunosorbent assay in immunodiagnosis is very important for batch, efficient and accurate detection. It can not only reduce the demand for detection manpower and greatly reduce the risk of detection contamination, but also reduce detection costs through large-scale rapid detection. With the development of automated equipment, the development of long-term and reliable detection equipment is very necessary.

[0003] Since the development of automated equipment for enzyme-linked immunosorbent assay, the reaction cups for enzyme-linked immunosorbent assay have been recycled, so the cleaning efficiency and continuous cleaning volume of the reaction cups have become important factors restricting the development of automated equipment. The Chinese utility model patent with a publication date of May 2, 2023 and a publication number of CN218956599U discloses an ultrasonic cleaning scheme to increase the cleaning efficiency, and designs multiple liquid separation structures to achieve bifurcation of the cleaning liquid into multiple cleaning branch channels, thereby achieving the cleaning of key components in multiple cleaning tanks. Because the ultrasonic action range is limited, the objects to be cleaned in this application are limited to specific objects such as sample needles, reagent needles, or stirring rods, and this scheme has limitations. The Japanese invention patent with a publication date of August 3, 2017 and a publication number of JP2017133970A discloses a pure cleaning liquid pumping liquid path system and a filling element scheme. In order to ensure the reliability of the cleaning operation and avoid the risk of system overflow, it is equipped with a waste liquid volume detection sensor at the storage position of the waste liquid storage tank, and can cooperate with the control module to accurately output the opening time of the cleaning channel when the outlet of the waste liquid storage tank is closed. The Chinese invention with a publication date of November 13, 2020 and a publication number of CN111936864A, discloses a solution that focuses on the protection of the cleaning method of the cleaning needle in the cleaning head. It uses the suction needle not to operate for a certain period of time to allow the filling needle to fill a predetermined volume of cleaning liquid, and the needle can be completely immersed in the cleaning liquid, so that the cleaning needle in the cleaning head can be cleaned more thoroughly. The Chinese invention patent with a publication date of July 21, 2023 and a publication number of CN110062886B discloses a solution in which at least one of the two suction needles is configured to be able to move up and down, and the relative distance between the two suction needle needles is changed during the cleaning liquid filling process, so that the two needles can be cleaned more thoroughly and reliably. The Chinese invention patent with the publication date of February 19, 2014 and the publication number of CN101688872B discloses a solution in which a first electrode is arranged in the waste liquid storage tank and a second electrode is arranged in the pipeline between the suction nozzle and the waste liquid storage tank. The electrostatic capacitance change between the two electrodes is used to determine whether the liquid circuit system is blocked, and the electrostatic capacitance threshold is set to continuously determine whether it is blocked. It has the advantage of continuous detection, but it is not applicable to some solutions and there are problems such as corrosion and contamination of the detection head during long-term operation. The Chinese invention with the publication date of September 11, 2013 and the publication number of CN101952730B introduces multiple suction needles of different lengths. On the one hand, it can increase the reliability of the liquid circuit operation, and on the other hand, multiple suction needles can also be used to determine whether it is blocked. Specifically, if a suction needle is blocked, the amount of liquid sucked by different suction needles in the same suction pressure drive or the pressure curve in the pipeline is different, so that it can be timely identified whether the system is blocked.

[0004] Although many different types of cleaning liquid circuits have been designed in the currently disclosed schemes, the research is mainly carried out from the aspects of anti-blocking, blockage detection and special cleaning of key components. In the enzyme-immune reaction, the reaction cup serves as a direct or indirect carrier for the binding of antigens or antibodies. It is reused in a new test during each reaction or after the reaction and detection are completed. In order to ensure the cleaning effect, each cleaning step needs to include multiple cleaning sub-steps, and more thorough cleaning in each cleaning sub-step is crucial. At the same time, the amount of cleaning liquid is crucial for continuous batch operation. The compatible operation of different washing liquids can also enable the equipment to match reaction vessels from different manufacturers. These problems all rely on the development of a new cleaning system and cleaning method. Summary of the invention

[0005] In order to solve the above problems existing in the prior art, the present invention is implemented by the following technical solutions:

[0006] In the first aspect of the present invention, an enzyme-free cleaning system is provided, comprising a rinsing barrel, a cleaning barrel group formed by a plurality of cleaning barrels, a waste liquid collection tank, a cleaning head, a cleaning tank, a filling pump, a control valve group and a vacuum pump, wherein the cleaning head comprises an injection needle and a liquid suction needle, the length of the liquid suction needle is greater than the length of the injection needle, the liquid suction needle can be fluidically connected to the waste liquid collection tank, the cleaning head can be matched with a cleaning position that can accommodate an enzyme-free reaction container provided in the cleaning tank, the filling pump can be respectively connected to the rinsing barrel or one of the cleaning barrels through the control valve group, the filling pump can also be fluidically connected to the injection needle, the cleaning tank is also provided with a suction port, the liquid suction needle and the suction port can both be fluidically connected to the waste liquid collection tank, the waste liquid collection tank is connected to the vacuum pump, and the vacuum pump can suck the waste liquid collection tank to make the waste liquid collection tank in a negative pressure state.

[0007] In one embodiment of the present invention, the cleaning tank is also configured with a rinsing position, which can temporarily store the cleaning head. In the rinsing step, the cleaning head is connected to the rinsing bucket, and the injection needle can be driven by the filling pump to inject the rinsing liquid into the rinsing position. After the rinsing is completed, under the action of the vacuum pump, the aspiration needle sucks the waste rinsing liquid into the waste liquid collection tank.

[0008] In one embodiment of the present invention, when the number of cleaning heads is N, the corresponding number of cleaning tanks is also N, each cleaning head cooperates with its corresponding cleaning tank, and the N filling pumps can be connected to the flushing bucket in the flushing step and to at least one of the cleaning buckets in the cleaning step through the control valve group, wherein N is an integer greater than or equal to 2.

[0009] In one embodiment of the present invention, the enzyme-free cleaning system also includes a relative motion driving mechanism, which can cause the cleaning head and the cleaning tank to produce relative motion. The relative motion driving mechanism includes a relative motion driving motor, the output shaft of the relative motion driving motor is connected to the driving wheel, and a transmission wheel is arranged at an interval with the driving wheel. A transmission belt is wound between the driving wheel and the transmission wheel, and the transmission belt is fixedly connected to a transmission connecting block, which is connected to the cleaning head or the cleaning tank.

[0010] In one embodiment of the present invention, the enzyme-free cleaning system further comprises a lifting drive mechanism, and before or after the cleaning solution is added, the cleaning head can be driven by the lifting drive mechanism to lift the tip of the aspiration needle to a position higher than the opening of the ELISA reaction container.

[0011] In one embodiment of the present invention, the lifting drive mechanism includes a lifting motor connected to a lifting base plate, the output end of the lifting motor is connected to a lifting drive gear, the lifting drive gear is meshedly connected to a transmission rack, the transmission rack is connected to a cleaning head, and a slide rail is also fixedly connected to the lifting base plate. The cleaning head is directly or indirectly connected to a lifting connection block, the lifting connection block is provided with a slide groove, and the lifting connection block is connected to the slide rail through an engagement connection between the slide groove.

[0012] In one embodiment of the present invention, the cleaning head is connected to the lifting base plate via a cleaning head balancing member, two ends of the cleaning head balancing member are respectively connected to the cleaning head and the lifting base plate, and the elastic coefficient of the cleaning head balancing member is 0.13-0.22N / mm.

[0013] In the second aspect of the present invention, an automated ELISA detection device is provided, which includes the ELISA cleaning system, and also includes an ELISA reaction container for receiving samples, an oscillating incubation unit and a reaction detection unit. The oscillating incubation unit can incubate and oscillate the ELISA reaction container at a constant temperature when performing an ELISA reaction in the ELISA reaction container, the reaction detection unit can detect the ELISA reaction in the ELISA reaction container, and the ELISA cleaning system can clean the ELISA reaction container.

[0014] In the third aspect of the present invention, a method for cleaning an enzyme-free reaction container is provided, the method for cleaning an enzyme-free reaction container adopts the enzyme-free cleaning system, and the enzyme-free cleaning system also includes a relative motion drive mechanism and a lifting drive mechanism, the method includes: in the cleaning step, the relative motion drive mechanism causes the cleaning head and the cleaning tank to move relative to each other, so that the cleaning head is located above at least part of the enzyme-free reaction container in the cleaning tank, and a filling pump is connected to a cleaning barrel, so that the injection needle can fill the cleaning liquid into the enzyme-free reaction container; after the cleaning is completed, the lifting drive mechanism drives the cleaning head to descend until the liquid pipetting needle approaches or contacts the bottom of the enzyme-free reaction container, and the waste liquid collection tank is in a negative pressure state under the action of the vacuum pump, and the liquid pipetting needle discharges the cleaning liquid waste liquid in the enzyme-free reaction container into the waste liquid collection tank, and the suction port can fluidically connect the overflow liquid to the waste liquid collection tank, wherein, before the cleaning liquid is added or when the filling is completed, the lifting drive mechanism drives the cleaning head to rise, so that the needle tip of the liquid pipetting needle is higher than the position of the mouth of the enzyme-free reaction container.

[0015] In one embodiment of the present invention, the enzyme-free reaction container cleaning method also includes a flushing step: the relative motion drive mechanism drives the cleaning head to move above the flushing position in the cleaning tank, the filling pump is connected to the flushing bucket, and the injection needle injects the flushing liquid into the flushing position. After the flushing is completed, the waste liquid collection tank is in a negative pressure state under the action of the vacuum pump, and the aspiration needle sucks the flushing liquid waste liquid after the flushing operation into the waste liquid collection tank, wherein the flushing position is not connected to the cleaning position.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. The enzyme-free cleaning system provided by the present invention is equipped with a flushing bucket and multiple cleaning buckets. The filling pump can be connected to the flushing bucket or one of the cleaning buckets respectively through the control valve group. The filling pump is also connected to the injection needle of the cleaning head. The injection needle fills the cleaning liquid into the enzyme-free reaction container in the cleaning position of the cleaning tank. The liquid suction needle sucks the filled cleaning liquid waste liquid. After several filling and suction actions, the enzyme-free reaction container is automatically cleaned. The cleaning tank also includes a suction port connected to the waste liquid collection tank. Even if the cleaning liquid is too much and may overflow, it can be sucked and moved in time and effectively. By configuring a flushing position that is not connected to the cleaning position, a temporary storage position can be provided for the cleaning head, and the flushing and cleaning operation of the cleaning head can be interspersed without interference to avoid the risk of clogging of the cleaning head. The multiple cleaning barrels configured can increase the amount of cleaning liquid stored in the system in one case to ensure the running time of the continuous detection of the system. In another case, different types of cleaning liquids can be configured for different cleaning barrels to be compatible with enzyme-free reaction containers of different manufacturers, thereby improving the compatibility and versatility of equipment in the enzyme-free cleaning system. In addition, the number of cleaning heads of the enzyme-free cleaning system provided by the present invention can be configured to be one or more. When the number of cleaning heads is multiple, multiple cleaning heads can improve the cleaning efficiency and make the operation efficiency of the enzyme-free detection equipment higher.

[0018] 2. The enzyme-free cleaning system provided by the present invention configures the relative motion drive mechanism as a pulley transmission structure driven by a motor, and the overall configuration is simple. The relative motion drive mechanism can reliably and smoothly change the position of the cleaning tank or the cleaning head, so that the cleaning operation and the flushing operation can be smoothly and reliably interspersed. The lifting drive mechanism is configured as a gear rack meshing transmission structure driven by the lifting motor drive output. The lifting drive mechanism lifts the cleaning head to a position where the needle tip of the liquid aspiration needle is higher than the position of the enzyme-free reaction container mouth before or at the end of the cleaning liquid filling, so as to ensure that the cleaning liquid in the enzyme-free reaction container is filled to a full state, greatly reducing the risk of wall contamination during the enzyme-free reaction oscillation process, ensuring the cleaning effect, and the cleaning head can be reliably limited by the connection setting of the cleaning head balance member and the lifting substrate, avoiding the risk of damage such as the cleaning head falling off and hitting the needle caused by the motor power failure.

[0019] 3. The automated ELISA detection device provided by the present invention can reliably perform immunodiagnosis of samples for a longer period of time, and realizes a fully automated process of ELISA reaction, detection, and container cleaning.

[0020] 4. In the ELISA reaction container cleaning method provided by the present invention, when executing the cleaning step, before or at the end of the filling of the cleaning solution, the cleaning head can be driven by the lifting drive mechanism and lifted to a position where the tip of the aspiration needle is higher than the mouth of the ELISA reaction container, thereby ensuring that the ELISA reaction container can be cleaned efficiently and thoroughly. The configuration of the flushing position can provide a temporary storage position for the cleaning head, and can also promptly and effectively clear possible blockages from the cleaning head, thereby making the system operation more reliable.

[0021] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the system structure of an enzyme-free cleaning system provided in Example 1;

[0023] Figure 2 yes Figure 1 A schematic diagram of the overall structure of a cleaning module and a washing liquid module in an enzyme-free cleaning system provided;

[0024] Figure 3 is a schematic diagram of the system structure of another enzyme-free cleaning system provided in Example 1;

[0025] Figure 4 This is a schematic diagram of the system structure of another enzyme-free cleaning system provided in Example 1;

[0026] Figure 5 yes Figure 3 A schematic diagram of the overall structure of the cleaning module and the washing liquid module in the enzyme-free cleaning system provided;

[0027] Figure 6 It is a structural schematic diagram of the liquid barrel receiving position in the washing liquid module of the enzyme-free cleaning system provided in Example 1;

[0028] Figure 7 It is a schematic diagram of the structure of the pump valve assembly in the washing liquid module of the enzyme-free washing system provided in Example 1;

[0029] Figure 8 This is a schematic diagram of the structure of the washing liquid module after removing the partition plate and the side plate in the enzyme-free washing system provided in Example 1;

[0030] Fig. 9 It is a schematic diagram of the three-dimensional structure of the cleaning module in the enzyme-free cleaning system provided in Example 1;

[0031] Fig.10 It is a structural schematic diagram of a relative motion driving mechanism in an enzyme-free cleaning system provided by the present invention that connects a cleaning head and drives the cleaning head to move;

[0032] Fig.11 It is a schematic diagram of a relative motion driving mechanism connected to a cleaning tank and driving the cleaning tank to move in an enzyme-free cleaning system provided by another embodiment of the present invention;

[0033] Fig.12 It is a three-dimensional structural schematic diagram of the lifting and driving mechanism of the cleaning module in the enzyme-free cleaning system provided in Example 1;

[0034] Fig.13 It is a schematic diagram of the configuration structure of the cleaning head balance member in the cleaning module in the enzyme-free cleaning system provided in the first embodiment;

[0035] Fig.14 (a) shows a schematic diagram of the process in which the lifting drive mechanism directly lifts the cleaning head to a specific height before the cleaning liquid is added, and the injection needle is connected to the cleaning barrel to start filling the cleaning liquid into the ELISA reaction container;

[0036] Fig.14 (b) in the figure shows a schematic diagram of the process in which the cleaning head is kept at a specific height and the injection needle continuously injects the cleaning solution into the ELISA reaction container when the cleaning solution is added; Fig.14 (c) in the figure shows a schematic diagram of the process in which the cleaning head is kept at a specific height and the injection needle stops filling the cleaning solution into the ELISA reaction container at the end of the filling;

[0037] Fig.15 (a) shows a schematic diagram of the process in which, before the cleaning solution is added, the lifting drive mechanism drives the cleaning head to descend so that at least part of the liquid aspiration needle extends into the ELISA reaction container, and the liquid injection needle is connected to the cleaning barrel to start filling the cleaning solution into the ELISA reaction container; Fig.15(b) in the figure shows a schematic diagram of the process in which when the cleaning liquid is added, the lifting mechanism drives the cleaning head to move upward and lift, and the injection needle continuously fills the cleaning liquid into the ELISA reaction container; Fig.15 (c) in the figure shows a schematic diagram of the process in which, at the end of filling, the aspiration needle is driven to a position where the tip of the aspiration needle is higher than the mouth of the ELISA reaction container, and the injection needle stops filling the cleaning solution into the ELISA reaction container.

[0038] Description of reference numerals:

[0039] 110-cleaning head; 111-liquid aspiration needle; 112-liquid injection needle; 114-liquid aspiration connector; 115-liquid injection connector;

[0040] 120-cleaning tank; 121-suction port; 122-rinsing position; 123-waste liquid connector; 124-liquid adding connector;

[0041] 131-relative motion driving motor; 132-driving wheel; 133-transmission belt; 134-transmission wheel; 135-transmission connecting block; 136-guide rail;

[0042] 141-lifting motor; 142-lifting driving gear; 143-transmission rack; 144-lifting connecting block; 145-slide rail; 146-cleaning head balance member; 148-sensing member; 149-sensing block;

[0043] 200-ELISA reaction container;

[0044] 31-rinsing bucket receiving position; 310-rinsing bucket; 320-cleaning bucket; 330-filling pump; 321-first cleaning bucket receiving position; 322-second cleaning bucket receiving position; 323-third cleaning bucket receiving position; 34-vacuum pump; 35-waste liquid collection tank; 36-control valve group; 371-waste liquid pump; 38-waste liquid storage tank. DETAILED DESCRIPTION

[0045] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the scheme according to the present invention is described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0046] The above and other technical contents, features and effects of the present invention are clearly presented in the following detailed description of the specific implementation modes in conjunction with the accompanying drawings. Through the description of the specific implementation modes, the technical means and effects adopted by the present invention to achieve the predetermined purpose can be more deeply and specifically understood. However, the attached drawings are only for reference and explanation purposes and are not used to limit the technical solutions of the present invention.

[0047] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants are intended to cover non-exclusive inclusion, so that an article or device including a series of elements includes not only those elements, but also other elements that are not explicitly listed. In the absence of more restrictions, the elements defined by the statement "including one..." do not exclude the existence of other identical elements in the article or device including the elements.

[0048] Embodiment 1

[0049] Please refer to Figure 1 , Figure 1 1 is a schematic diagram of the system structure of an enzyme-free cleaning system provided in Example 1, wherein the enzyme-free cleaning system includes a rinse barrel 310, a cleaning barrel group formed by a plurality of cleaning barrels 320, a waste liquid collection tank 35, a cleaning head 110, a cleaning tank 120, a filling pump 330, a control valve group 36 and a vacuum pump 34, the cleaning head 110 includes an injection needle and a liquid suction needle, the length of the liquid suction needle is greater than the length of the injection needle, the liquid suction needle can be fluidically connected to the waste liquid collection tank 35, the cleaning head 110 can cooperate with the cleaning tank 120 that can accommodate the enzyme-free reaction container 200 In the cleaning position, the filling pump 330 can be connected to the flushing barrel 310 or one of the cleaning barrels 320 respectively through the control valve group 36. The filling pump 330 can also be fluidically connected to the injection needle. The cleaning tank 120 is also provided with a suction port. The suction needle and the suction port can be fluidically connected to the waste liquid collection tank 35. The waste liquid collection tank 35 is connected to the vacuum pump 34. The vacuum pump 34 can suck the waste liquid collection tank 35 to make the waste liquid collection tank 35 in a negative pressure state. The suction needle connected to the waste liquid collection tank 35 is also in a negative pressure state, thereby producing a suction effect on the liquid in contact with the suction needle.

[0050] In this embodiment, the enzyme-free cleaning system includes one flushing barrel 310, and the number of cleaning barrels 320 is shown as three. The cleaning reagent in the cleaning barrel 320 is different from the flushing reagent in the flushing barrel 310. Configuring the cleaning barrel 320 to be two or more than two can be suitable for continuous cleaning scenarios. The enzyme-free detection equipment currently developed usually needs to continuously perform long-term large-scale detection. The liquid circuit system of a single cleaning barrel has become increasingly unsuitable for automated batch detection scenarios. In one scenario, when the cleaning reagent in one cleaning barrel is used up, the liquid circuit system formed by the filling pump, the control valve group and the cleaning head can be connected to another cleaning barrel to continue to complete the cleaning operation, thereby reducing manual operations during the detection process. The flushing barrel 310 and the cleaning barrel 320 can be connected to the control valve group 36. In order to ensure that the cleaning liquid or the flushing liquid is reliably supplied, a plurality of two-way valves corresponding to the flushing barrel 310 and the cleaning barrel 320 are arranged in the control valve group 36. The two-way valve can be a public type of electromagnetic two-way valve or a pneumatic two-way valve, etc. The output end of the control valve group can be finally connected to the filling pump 330 by a pipeline through a three-way connection. The filling pump 330 is a diaphragm pump or other types of positive displacement pumps. The filling pump 330 is also connected to the cleaning head 110 through a pipeline. Preferably, the filling pump 330 can be fluidically connected to the injection needle of the cleaning head 110 to achieve the addition of cleaning liquid or flushing liquid. The cleaning head 110 is also equipped with a liquid suction needle, which can be fluidically connected to the waste liquid collection tank 35. In order to ensure the reliable execution of filling and suction, the length of the liquid suction needle is greater than the length of the liquid suction needle. In this way, on the one hand, the used cleaning liquid can be removed more thoroughly and efficiently, and on the other hand, the excessive cleaning liquid can be timely and effectively sucked during the cleaning liquid filling process. A cleaning tank 120 is provided in conjunction with the cleaning head 110, and a cleaning position is provided in the cleaning tank 120. In the cleaning step of cleaning the cleaning position, the injection needle of the cleaning head 110 is connected to a cleaning barrel 320, and the cleaning head 110 can be matched to the cleaning position of the cleaning tank 120. The filling pump 330 can drive the cleaning liquid to be filled into the enzyme-free reaction container in the cleaning position through the injection needle. The cleaning tank is also provided with a suction port, which is fluidically connected to a waste liquid collection tank 35, and the waste liquid collection tank 35 is connected to a vacuum pump 34. When the cleaning position is finished, the waste liquid collection tank 35 is in a negative pressure state under the action of the vacuum pump 34, and the liquid suction needle collects the waste liquid after cleaning into the waste liquid collection tank 35, and the suction port can recover the overflowed waste liquid into the waste liquid collection tank 35.

[0051] Furthermore, the cleaning tank 120 is also configured with a rinsing position 122, which can temporarily store the cleaning head 110. In order to reduce the risk of cross contamination, the rinsing position 122 is not connected to the cleaning position. In the rinsing step of rinsing the rinsing position 122, the cleaning head 110 is connected to the rinsing barrel 310, and the injection needle can be driven by the filling pump 330 to inject rinsing liquid into the rinsing position. After the rinsing is completed, under the action of the vacuum pump 34, the aspiration needle sucks the waste rinsing liquid into the waste liquid collection tank 35, that is, the waste liquid collection tank 35 is in a negative pressure state, and the aspiration needle sucks the waste rinsing liquid after the rinsing operation into the waste liquid collection tank 35.

[0052] Furthermore, in order to reduce the operating noise of the vacuum pump 34, the vacuum pump 34 is connected to a muffler element, and the waste liquid collection tank 35 may also be equipped with a pressure gauge to detect the negative pressure value therein. Preferably, in order to ensure that the liquid in the waste liquid collection tank 35 is monitored in time and to ensure the reliability of the vacuum pump 34 connected to the waste liquid collection tank 35, a liquid level sensing unit is configured in the waste liquid collection tank 35. Preferably, the system is also equipped with a waste liquid pump 371, one end of the waste liquid pump 371 is connected to the waste liquid collection tank 35, and the other end is connected to the waste liquid storage tank 38. With the cooperation of the liquid level sensing unit, the waste liquid pump 371 is operated at fixed time or irregular time intervals to transfer the waste liquid collected in the waste liquid collection tank 35 to the waste liquid storage tank 38.

[0053] Please refer to Figure 2 , Figure 2 yes Figure 1 A schematic diagram of the overall structure of a cleaning module and a washing liquid module in an enzyme-free cleaning system is provided, wherein the enzyme-free cleaning system is provided with a cleaning module and a washing liquid module, and its system structure corresponds to Figure 1In the system structure shown, the washing liquid module is provided with a liquid barrel, a liquid barrel receiving position and a pump valve assembly, and the washing liquid module includes a washing head 110 and a washing tank 120. In this embodiment, the liquid barrel includes a washing barrel 320 and a rinsing barrel 310. Specifically, the washing liquid module is provided with a rinsing barrel receiving position 31 and three washing barrel receiving positions, and the three washing barrel receiving positions are respectively a first washing barrel receiving position 321, a second washing barrel receiving position 322, and a third washing barrel receiving position 323, and each washing barrel receiving position can receive a washing barrel 320. The rinsing barrel receiving position 31 receives the rinsing barrel 310, and the rinsing barrel receiving position 31 and the three washing barrel receiving positions are arranged in parallel. The liquid barrel in the washing liquid module is connected to the washing head 110 by a pipeline as described above, and the washing head 110 is matched to the washing position of the washing tank 120. The washing position can form a temporary storage position for the washing head 110 so that the washing head 110 can be temporarily stored, and the washing position is configured as a groove structure with a certain depth. When the washing head 110 is connected to the washing barrel 310, the filling pump 330 can drive the washing liquid in the washing barrel 310 to be injected into the washing position. Specifically, the filling pump 330 fills the washing liquid into the washing position through the injection needle, and the longer suction needle can contact the washing liquid in the washing position more quickly, so that the suction needle cooperates with the injection needle to complete the washing effect of continuous injection and continuous suction in the washing position, and the washing head 110 can also suck the washing liquid that has completed the washing operation to the waste liquid collection tank 35.

[0054] Furthermore, the enzyme-free cleaning system provided in this embodiment also supports a parallel enzyme-free reaction container cleaning solution. When the number of cleaning heads 110 is N, N is an integer greater than or equal to 2, and the number of corresponding cleaning tanks 120 is also N, each cleaning head 110 cooperates with its corresponding cleaning tank 120, and the N filling pumps 330 can be connected to the flushing barrel 310 in the flushing step through the control valve group 36, and connected to at least one of the cleaning barrels 320 in the cleaning step.

[0055] For details, please refer to Figure 3 and Figure 4 , Figure 3 is a schematic diagram of the system structure of another enzyme-free cleaning system provided in Example 1, Figure 3 FIG. 2 shows a parallel ELISA reaction vessel cleaning solution performed by two cleaning tanks 120 and two cleaning heads 110. Figure 4 is a schematic diagram of the system structure of another enzyme-free cleaning system provided in Example 1, Figure 4The parallel type enzyme-free reaction container cleaning scheme performed by three cleaning tanks 120 coordinated with three cleaning heads 110 is shown in the figure. The multiple cleaning tanks are all equipped with suction ports. In each parallel type enzyme-free reaction container cleaning scheme, all cleaning tanks are connected to the same waste liquid collection tank 35. The same number of filling pumps 330 corresponding to the cleaning heads 110 are also configured in the liquid circuit system. The independent filling pumps 330 can achieve efficient cleaning of multiple enzyme-free reaction containers to be cleaned. In some other embodiments, at least two of the multiple cleaning barrels 320 include cleaning barrels 320 configured with two different cleaning reagents. This arrangement allows the cleaning liquid corresponding to different manufacturers to be configured in the cleaning barrel 320 when using enzyme-free reaction containers of different manufacturers to perform multiple types of enzyme-free detection, so that N filling pumps 330 can be connected to at least one of the cleaning barrels 320 through the control valve group 36 in the cleaning step, so that the versatility and compatibility of the detection equipment are stronger. Of course, if space permits, more cleaning heads and corresponding cleaning tanks can be configured in parallel, and the specific number is not limited.

[0056] Please refer to Figure 5 , Figure 5 yes Figure 3 The overall structure diagram of the cleaning module and the washing liquid module in the enzyme-free cleaning system provided is as follows: Figure 2 The structure of the enzyme-free cleaning system shown in FIG. 1 is provided with one more cleaning module. Specifically, the enzyme-free cleaning system includes two cleaning heads 110, which are designed with two cleaning tanks 120. A plurality of enzyme-free reaction containers 200 arranged in an array are arranged in the cleaning tanks 120, for example, 96 enzyme-free reaction containers 200. In one cleaning operation, the two cleaning heads 110 can be connected to the same cleaning barrel 320 to perform efficient cleaning. In another cleaning operation, the two cleaning heads 110 can be connected to two different cleaning barrels 320 respectively to perform more compatible cleaning of reaction containers of different manufacturers or different types. The two filling pumps 330 can be connected to the flushing barrel 310 in the flushing step through the control valve group 36, and connected to at least one of the cleaning barrels 320 in the cleaning step.

[0057] Based on the parallel ELISA reaction container cleaning scheme, the cleaning operation and the flushing operation can be performed interspersed. Preferably, the ELISA cleaning system is also equipped with a blockage detection element, which can timely and effectively detect whether the system is blocked, and then reasonably arrange the flushing operation procedure when the blockage detection value exceeds the threshold. In order to simplify the system, the flushing operation can be performed interspersed with fixed intervals or non-fixed intervals to ensure that the liquid system is flushed more frequently to improve the reliability of the system operation.

[0058] This embodiment configures a flushing bucket and multiple cleaning buckets in the enzyme-free cleaning system, and the filling pump can be connected to the flushing bucket or one of the cleaning buckets through the control valve group. The filling pump is also connected to the injection needle of the cleaning head. The injection needle fills the cleaning liquid into the enzyme-free reaction container in the cleaning position of the cleaning tank, and the liquid suction needle sucks the waste liquid of the filled cleaning liquid. After several times of filling and suction, the enzyme-free reaction container is automatically cleaned. The cleaning tank also includes a suction port connected to the waste liquid collection tank, and even if the cleaning liquid is too much and causes a possible overflow, it can be sucked and moved in time and effectively. By configuring a flushing position that is not connected to the cleaning position, a temporary storage position can be provided for the cleaning head, and the flushing and cleaning operation of the cleaning head can be interspersed without interference, avoiding the risk of clogging of the cleaning head. The multiple cleaning barrels configured can increase the amount of cleaning liquid stored in the system in one case to ensure the running time of the system's continuous detection. In another case, different types of cleaning liquids can be configured for different cleaning barrels to be compatible with enzyme-free reaction containers of different manufacturers, thereby improving the compatibility and versatility of equipment in the enzyme-free cleaning system. In addition, the number of cleaning heads of the enzyme-free cleaning system provided in this embodiment can be configured to be one or more. When the number of cleaning heads is multiple, multiple cleaning heads can improve the cleaning efficiency and make the operation efficiency of the enzyme-free detection equipment higher.

[0059] Please refer to Figure 6 , Figure 6 It is a structural schematic diagram of the liquid barrel receiving position in the washing liquid module in the enzyme-free cleaning system provided in the first embodiment. Specifically, the washing barrel receiving position and the rinsing barrel receiving position 31 are both liquid barrel receiving positions. The washing liquid module is centrally and parallelly provided with multiple washing barrel receiving positions and rinsing barrel receiving positions 31, which can receive rinsing barrels 310 and multiple washing barrels 320 respectively. In order to ensure that the rinsing barrels 310 in the washing barrel receiving position 31 and the washing barrels 320 in the washing barrel receiving position can be accurately quantified, and to make the filling amount control more accurate, a weight detection unit is also configured in each liquid barrel receiving position, and the weight detection unit can accurately detect the weight change of the reagent barrel in the receiving position. Here, the weight detection unit can be an electronic gravity sensor, and the weight change data can be digitized and signaled to achieve accurate filling amount control.

[0060] The pump valve assembly is arranged on the other side opposite to the liquid barrel receiving position of the cleaning liquid module. Figure 7 , Figure 7This is a schematic diagram of the structure of the pump valve assembly in the washing liquid module in the enzyme-free cleaning system provided in Example 1. In order to ensure the compactness and configuration reliability of the washing liquid module, the liquid barrel receiving position and the pump valve assembly are arranged on a common bottom plate. The pump valve assembly is also arranged with a side plate that partially or completely closes the pump valve assembly. The pump valve assembly includes a vacuum pump 34. In order to reduce the vibration and noise impact of the operation of the vacuum pump 34, the vacuum pump 34 is arranged on a vibration cushion. In order to ensure the reliability of the system configuration, all control valves are centrally configured to form a control valve group 36. The control valve group 36 contains multiple independent two-way valves. This configuration makes the inspection and maintenance of the control valve group 36 simpler. The control valve group 36 is configured on a bracket with a predetermined height to reduce various interferences during operation. Furthermore, a partition is arranged between the receiving position of the liquid barrel and the pump-valve assembly, and a plurality of filling pumps 330 and a waste liquid pump 371 are fixedly installed in the partition. Setting the filling pump 330 and the waste liquid pump 371 as the same type of pump can reduce costs. The vacuum pump 34 and the liquid pump are arranged on different substrates to reduce the risk of resonance when they are arranged in the same module. In order to reduce the waste of space, the pump-valve assembly also includes a waste liquid collection tank 35. The vacuum pump 34 and the waste liquid collection tank 35 are close to each other, and the connection pipeline configuration between the two is also simpler, making the system configuration more reliable.

[0061] Please refer to Figure 8 , Figure 8 It is a schematic diagram of the structure of the washing liquid module after removing the partition plate and the side plate in the enzyme-free cleaning system provided in Example 1. After removing the partition plate and the side plate, the structure of the washing liquid module is observed. It can be seen that all liquid pumps are connected through the liquid pump configuration plate and the partition plate to achieve modular centralized configuration of the liquid pump, and a vibration damping pad is configured between each liquid pump and the liquid pump configuration plate. Various liquid barrels contain the same connector, each connector is connected to the partition plate and then connected to the liquid pump and / or the control valve group 36 through a pipeline. Various drive circuit boards and control boards can be configured accordingly and are not limited here.

[0062] Please refer to Fig. 9 and Fig.10 , Fig. 9 is a schematic diagram of the three-dimensional structure of the cleaning module in the enzyme-free cleaning system provided in Example 1, Fig.10It is a structural schematic diagram of a relative motion driving mechanism in an enzyme-free cleaning system provided by the present invention that connects a cleaning head and drives the cleaning head to move. The enzyme-free cleaning system also includes a relative motion driving mechanism. The relative motion of the cleaning head 110 and the cleaning tank 120 depends on the drive of the relative motion driving mechanism, that is, the relative motion driving mechanism can make the cleaning head 110 and the cleaning tank 120 produce relative motion. The cleaning tank 120 itself includes a cleaning position that can place the enzyme-free reaction container 200, and a flushing position 122 arranged at intervals from the cleaning position. The cleaning head 110 can move relative to the cleaning tank 120, so that the cleaning head 110 can cooperate with the flushing position 122 to temporarily store or perform a flushing operation. In this way, even if there is a risk of dripping in the flushing position, the cleaning position of the reaction container will not be contaminated, and the cleaning head 110 can cooperate with the cleaning position to perform a reaction cup cleaning operation. Specifically, the relative motion driving mechanism includes a relative motion driving motor 131, the output shaft of the relative motion driving motor 131 is connected to a driving wheel, a transmission wheel 134 is arranged at an interval with the driving wheel, a transmission belt 133 is wound between the driving wheel and the transmission wheel 134, a transmission connecting block 135 is fixedly connected at one position of the transmission belt 133, and the transmission connecting block 135 is connected to the cleaning head 110 or the cleaning tank 120. The rotational motion of the relative motion driving motor 131 causes the cleaning head 110 and the cleaning tank 120 to produce relative motion, thereby changing the matching position of the two.

[0063] In this embodiment, the relative motion driving mechanism is connected to the cleaning head and drives the cleaning head to move, so that the cleaning head 110 can move relative to the cleaning tank 120. Fig.10 As shown, the output shaft of the relative motion driving motor 131 is connected to the driving wheel 132, and a transmission wheel 134 is arranged at a preset distance from the driving wheel 132, and a transmission belt 133 is arranged between the two wheels. In order to ensure the reliability of the transmission, a transmission connection block 135 is fixedly connected to the lower side of the transmission belt, and the transmission connection block 135 is connected to the cleaning head 110. The rotation of the driving motor can drive the cleaning head to cooperate with different positions of the flushing position or the cleaning position. The cleaning head 110 includes a liquid suction joint 114 connected to the liquid suction needle and a liquid injection joint 115 connected to the liquid injection needle. In order to ensure that the possible overflow liquid can be discharged in time and effectively, the cleaning tank is also provided with a suction port 121 that can be connected to the cleaning position. The suction port 121 and the liquid suction joint 114 can be connected to the waste liquid joint 123 through a pipeline. The waste liquid joint 123 is liquid-connected to the waste liquid collection tank 35, so that the possible overflow liquid in the cleaning position of the cleaning tank and the cleaning liquid in the reaction cup can be efficiently and timely removed. The liquid injection joint 115 can be connected to the liquid addition joint 124 through a pipeline.

[0064] Please refer to Fig.11 , Fig.111 is a schematic diagram of an enzyme-free cleaning system provided by another embodiment of the present invention, in which a relative motion driving mechanism is connected to a cleaning tank and drives the cleaning tank to move. In another embodiment of the present invention, the relative motion driving mechanism drives the cleaning tank to move by connecting to the cleaning tank so that the cleaning head 110 can move relative to the cleaning tank 120. Its principle and structure are similar to Fig.10 The relative motion drive mechanism shown in FIG. 1 is connected to the cleaning head and drives the cleaning head to move, except that the transmission connection block 135 is fixedly connected to the cleaning tank 120. Similar structures and connections are not described here. Preferably, the enzyme-free cleaning system is also equipped with a guide rail 136 that can be directly or indirectly engaged with the transmission connection block 135, ensuring that the transmission connection block 135 can drive the cleaning head 110 or the cleaning tank 120 to move more smoothly and reliably.

[0065] Please refer to Fig.12 , Fig.12 It is a three-dimensional structural schematic diagram of the lifting drive mechanism of the cleaning module in the enzyme-free cleaning system provided in Example 1, the cleaning head 110 can be driven by the lifting drive mechanism to lift the liquid-absorbing needle tip above the position of the enzyme-free reaction container mouth, and the enzyme-free cleaning system also includes a lifting drive mechanism, which includes a lifting motor 141 connected to a lifting substrate, and the output end of the lifting motor 141 is connected to a lifting drive gear 142, and the lifting drive gear 142 is meshed and connected to a transmission rack 143, and the transmission rack 143 is connected to the cleaning head 110. The gear rack transmission can efficiently and accurately drive the cleaning head 110 to rise or fall, and realize a higher precision position control. Preferably, a slide rail 145 is also fixedly connected to the lifting substrate, and the cleaning head 110 is directly or indirectly connected to a lifting connection block 144, and the lifting connection block 144 is provided with a slide groove, and the lifting connection block 144 is connected by the slide groove and the slide rail 145. When the lifting motor 141 rotates to make the cleaning head 110 slide up and down along the lifting substrate, the aspiration needle 111 and the injection needle 112 configured on the cleaning head 110 are driven to perform corresponding lifting movements. Specifically, when the lifting motor 141 rotates in a first direction, the cleaning head 110 slides upward along the lifting substrate, and the aspiration needle 111 and the injection needle 112 configured on the cleaning head 110 are driven to perform an upward movement. When the lifting motor 141 rotates in an opposite second direction, the cleaning head 110 slides downward along the lifting substrate, and the aspiration needle 111 and the injection needle 112 configured on the cleaning head 110 are driven to perform a downward movement.

[0066] Further, the length of the liquid suction needle 111 is greater than the length of the liquid injection needle 112. Preferably, the length of the liquid injection needle 112 exposed to the outside of the cleaning head 110 matrix is ​​less than 1 / 2 of the length of the liquid suction needle 111 exposed to the outside of the cleaning head matrix. The length of the liquid injection needle 112 exposed to the outside of the cleaning head 110 matrix is ​​less than the length of the liquid suction needle 111 exposed to the outside of the cleaning head matrix. It can also be a ratio of 1 / 3, 1 / 4, 2 / 5, 1 / 5, etc., or it can be an approximate value of these ratios. When the liquid suction needle 111 aspirates the used waste liquid, even if the position driven by the lifting mechanism has a certain deviation, the length of the liquid injection needle 112 exposed to the outside of the cleaning head 110 matrix is ​​greater than the height difference between the length of the liquid suction needle 111 exposed to the outside of the cleaning head matrix, which greatly reduces the risk of the liquid injection needle 112 contacting the liquid during the liquid transfer process, and reduces the risk of contamination during the liquid injection and waste liquid suction and discharge process.

[0067] Furthermore, in order to ensure that the lifting drive mechanism can perform lifting and lowering drive reliably for a long time, the cleaning head 110 is also connected to a sensor block 149, and a sensor component 148 is arranged on the lifting substrate. The sensor block 149 and the sensor component 148 can be used as a limiter and can also act as a position calibration component to make the lifting drive mechanism more reliable and operate for a long time.

[0068] Please refer to Fig.13 , Fig.13 1 is a schematic diagram of the configuration structure of the cleaning head balance member in the cleaning module in the enzyme-free cleaning system provided in Example 1. The cleaning head 110 is connected to the lifting base plate through the cleaning head balance member 146, and the two ends of the cleaning head balance member 146 are respectively connected to the cleaning head 110 and the lifting base plate. In this embodiment, the cleaning head balance member 146 is a tension spring, and the two ends of the tension spring are respectively hooked and connected to the cleaning head 110 and the lifting base plate. Preferably, the cleaning head balance piece optimally selects a tension spring with an elastic coefficient of 0.13-0.22N / mm. Too small an elastic coefficient causes the gravity of the cleaning head 110 to be unable to be effectively balanced, which may cause a tilt problem in cooperation with the lifting substrate, resulting in serious movement resistance and wear of the two, which is unacceptable for enzyme-free reaction application scenarios that require precise driving of the cleaning head position. Too large an elastic coefficient will result in higher torque requirements for the drive motor, and there is also a risk of causing excessive force and deformation of the teeth of the gear rack, and it is not suitable for the long-term high-precision position control requirements of the enzyme-free cleaning head. This elastic coefficient ensures that the cleaning head balance piece 146 can reliably balance the weight of the cleaning head 110, and can also cooperate with the lifting drive mechanism to change the stretching state of the cleaning head 110 more labor-saving and efficient.

[0069] In the enzyme-linked reaction, oscillating incubation is very necessary. During this process, the test liquid in the enzyme-linked reaction container must not only be maintained in a constant temperature incubation environment for a long time, but also the enzyme-linked reaction container needs to be shaken or shaken to make the enzyme-linked reaction more fully and thoroughly executed. Shaking the reaction container during this process will put the reaction liquid in the reaction container in a certain state of motion, resulting in a high risk of the inner wall of the reaction container being contaminated with liquid. If the cleaning is not sufficient and thorough, there may be a risk of contamination. In the existing technology, most companies try to achieve more efficient cleaning by increasing the number of cleaning times. However, there is a small amount of cleaning liquid added, and the reaction cup always has a dead zone that cannot be cleaned. This problem is unacceptable for accurate detection. The enzyme-free cleaning system provided in this embodiment provides a variety of feasible cleaning schemes. The following describes two cleaning schemes for the enzyme-free cleaning system provided in this embodiment.

[0070] In the first cleaning solution provided by the enzyme-free cleaning system provided in this embodiment, please refer to Fig.14 , Fig.14 (a) in the figure shows a schematic diagram of the process in which the lifting drive mechanism directly lifts the cleaning head to a specific height before adding the cleaning liquid, and the injection needle is connected to the cleaning barrel to start filling the cleaning liquid into the ELISA reaction container. Fig.14 (b) in the figure shows a schematic diagram of the process in which the cleaning head is kept at a specific height and the injection needle continuously injects the cleaning liquid into the ELISA reaction container when the cleaning liquid is added. Fig.14 (c) in the figure shows a schematic diagram of the process in which the cleaning head is kept at a specific height and the injection needle stops filling the ELISA reaction container with cleaning solution at the end of the filling. In the ELISA cleaning system provided in this embodiment, before the cleaning solution is filled or at the end of the filling, the cleaning head 110 can be driven by the lifting drive mechanism to lift the tip of the aspiration needle 111 to a specific height, so that there is a certain height difference h between the tip of the aspiration needle 111 and the mouth of the ELISA reaction container, where h is a value associated with the diameter of the ELISA reaction container, and the specific height is higher than the position of the mouth of the ELISA reaction container. The specific height can ensure that the tip of the aspiration needle 111 is higher than the mouth of the reaction container. At this fixed height, the injection needle 112 is fluidically connected to the cleaning barrel 320 to fill the ELISA reaction container 200 with cleaning solution.

[0071] In the second cleaning solution provided by the enzyme-free cleaning system provided in this embodiment, please refer to Fig.15 , Fig.15 (a) in the figure shows a schematic diagram of a process in which, before the cleaning liquid is added, the lifting drive mechanism drives the cleaning head to descend so that at least part of the liquid aspiration needle extends into the ELISA reaction container, and the liquid injection needle is connected to the cleaning barrel to start filling the cleaning liquid into the ELISA reaction container. Fig.15 (b) in the figure shows a schematic diagram of the process in which the lifting mechanism drives the cleaning head to move upward and lift up, and the injection needle continuously fills the cleaning liquid into the ELISA reaction container when the cleaning liquid is added. Fig.15 (c) in the figure shows a schematic diagram of the process in which the liquid-pipetting needle is driven to a position where the tip of the liquid-pipetting needle is higher than the mouth of the ELISA reaction container at the end of the filling, and the liquid-injecting needle stops filling the ELISA reaction container with cleaning solution. In this scheme, the lifting drive mechanism can drive the cleaning head 110 to descend so that at least part of the liquid-pipetting needle 111 extends into the ELISA reaction container, and then the liquid-injecting needle 112 is fluidically connected to the cleaning barrel 320 to inject cleaning solution into the container, and at the same time, the lifting drive mechanism drives the cleaning head to move upward, so that at the end of the filling, the cleaning head 110 can be driven by the lifting drive mechanism to be lifted to a position where the tip of the liquid-pipetting needle 111 is higher than the mouth of the ELISA reaction container 200.

[0072] After studying these two schemes, it is found that in the enzyme-free reaction container with a smaller volume, more cleaning liquid can be added. In the initial stage of just being filled with cleaning liquid, the cleaning liquid does not overflow the reaction container immediately, but under the action of surface tension, more liquid can be injected into the reaction container, so that the liquid surface forms a protruding state, because the liquid suction needle is higher than the position of the reaction container mouth at this time, so that the liquid can maintain the protruding state, more liquid can be sucked away by the negative pressure of the liquid surface contacted by the liquid suction needle 111, and the state of the cleaning liquid protruding from the container mouth in the reaction container will be retained by the surface tension of the liquid surface, in this case, all the inner wall surfaces of the container can be soaked and cleaned, the cleaning efficiency is improved, and the pollution risk of multiple recycling of the enzyme-free reaction container is reduced. In different embodiments, the needle head of the liquid suction needle 111 is higher than the reaction container mouth, so that the cleaning liquid in the reaction container is filled with a protruding state in which the liquid surface protrudes from the reaction container mouth, and since the cleaning position of the cleaning tank in the present embodiment is also equipped with a suction port, even if there is a problem of a small amount of liquid overflowing the reaction container according to the method of the present invention, the waste liquid can be discharged in time and effectively, greatly reducing the pollution risk. The amount of cleaning liquid added to the ELISA reaction container may be 103%-110% of its nominal rated capacity to ensure that a sufficient amount of cleaning liquid is added to the ELISA reaction container without excessive filling leading to excessive overflow.

[0073] The enzyme-free cleaning system provided in this embodiment configures the relative motion drive mechanism as a pulley transmission structure driven by a motor, and the overall configuration is simple. The relative motion drive mechanism can reliably and smoothly change the position of the cleaning tank or the cleaning head, so that the cleaning operation and the flushing operation can be smoothly and reliably interspersed. The lifting drive mechanism is configured as a gear rack meshing transmission structure driven by the lifting motor drive output. The lifting drive mechanism lifts the cleaning head to a position where the needle tip of the liquid aspiration needle is higher than the position of the enzyme-free reaction container mouth before or at the end of the cleaning liquid filling, ensuring that the cleaning liquid in the enzyme-free reaction container is filled to a full state, greatly reducing the risk of wall contamination during the enzyme-free reaction oscillation process, ensuring the cleaning effect, and the cleaning head can be reliably limited by the connection setting of the cleaning head balance member and the lifting substrate, avoiding the risk of damage such as the cleaning head falling off and hitting the needle caused by the motor power failure.

[0074] Embodiment 2

[0075] An automated ELISA device comprises the ELISA cleaning system of the first embodiment, and further comprises an ELISA reaction container for receiving a sample, an oscillating incubation unit, a reaction detection unit, etc. Specifically, the oscillating incubation unit can incubate and oscillate the ELISA reaction container at a constant temperature when the ELISA reaction container performs an ELISA, the reaction detection unit can detect the ELISA in the ELISA reaction container, and the ELISA cleaning system can clean the ELISA reaction container, thereby realizing a fully automated execution process from sample reception to test result output and container cleaning.

[0076] The automated ELISA test device provided in this embodiment can reliably perform immunodiagnosis of samples for a longer period of time, and realizes a fully automated process of ELISA reaction, detection, and container cleaning.

[0077] Embodiment 3

[0078] The present invention also discloses an enzyme-free reaction container cleaning method, which is to perform an enzyme-free reaction container cleaning method based on the enzyme-free cleaning system provided in Example 1, that is, the cleaning head is provided with a relative motion driving mechanism and a lifting driving mechanism, and the flushing position is not connected to the cleaning position, and the method comprises:

[0079] In the cleaning step, the relative motion driving mechanism causes the cleaning head and the cleaning tank to move relative to each other, so that the cleaning head is located above at least part of the ELISA reaction container in the cleaning tank, and the filling pump is connected to one of the cleaning barrels, so that the filling needle can fill the cleaning liquid into the ELISA reaction container;

[0080] Before or after the cleaning solution is added, the lifting drive mechanism drives the cleaning head to rise, so that the tip of the aspiration needle is higher than the position of the ELISA reaction container opening;

[0081] After cleaning is completed, the lifting drive mechanism drives the cleaning head down until the pipette needle is close to or in contact with the bottom of the ELISA reaction container. Under the action of the vacuum pump, the waste liquid collection tank is in a negative pressure state. The pipette needle discharges the cleaning liquid waste in the ELISA reaction container into the waste liquid collection tank, and the suction port can fluidically connect the overflow liquid to the waste liquid collection tank.

[0082] Furthermore, the method also includes a flushing step, which specifically includes: a relative motion drive mechanism drives the cleaning head to move above the flushing position in the cleaning tank, a filling pump is connected to the flushing barrel, and an injection needle injects flushing liquid into the flushing position. After flushing is completed, the waste liquid collection tank is in a negative pressure state under the action of the vacuum pump, and the aspiration needle sucks the flushing liquid waste after the flushing operation into the waste liquid collection tank.

[0083] The ELISA reaction container cleaning method provided in this embodiment, when executing the cleaning step, before or at the end of the filling of the cleaning liquid, the cleaning head can be driven by the lifting drive mechanism and lifted to a position where the needle tip of the aspiration needle is higher than the mouth of the ELISA reaction container, thereby ensuring that the ELISA reaction container can be cleaned efficiently and thoroughly. The configuration of the flushing position can provide a temporary storage position for the cleaning head, and can also promptly and effectively clear possible blockages from the cleaning head, thereby making the system operation more reliable.

[0084] The above contents are further detailed descriptions of the present invention in combination with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. An enzyme-free cleaning system, characterized in that: The invention comprises a flushing bucket (310), a cleaning bucket group formed by a plurality of cleaning buckets (320), a waste liquid collection tank (35), a cleaning head (110), a cleaning tank (120), a filling pump (330), a control valve group (36) and a vacuum pump (34), wherein: The cleaning head (110) comprises an injection needle and a suction needle. The length of the suction needle is greater than that of the injection needle. The suction needle can be fluidically connected to the waste liquid collection tank (35). The cleaning head (110) can be matched with a cleaning position that can accommodate an enzyme-free reaction container (200) and is provided in the cleaning tank (120). The filling pump (330) can be respectively connected to the flushing bucket (310) or one of the cleaning buckets (320) through a control valve group (36). The filling pump (330) can also be fluidically connected to the injection needle. The cleaning tank (120) is also provided with a suction port. The suction needle and the suction port can both be fluidically connected to the waste liquid collection tank (35). The waste liquid collection tank (35) is connected to a vacuum pump (34). The vacuum pump (34) can suck the waste liquid collection tank (35) to make the waste liquid collection tank (35) in a negative pressure state.

2. The enzyme-free cleaning system according to claim 1, characterized in that: The cleaning tank (120) is also provided with a flushing position (122) which can temporarily store the cleaning head (110). During the flushing step, the cleaning head (110) is connected to the flushing barrel (310), and the injection needle can be driven by the injection pump (330) to inject flushing liquid into the flushing position. After the flushing is completed, under the action of the vacuum pump (34), the suction needle sucks the flushing liquid waste into the waste liquid collection tank (35).

3. The enzyme-free cleaning system according to claim 2, characterized in that: When the number of cleaning heads (110) is N, the number of corresponding cleaning tanks (120) is also N, and each cleaning head (110) cooperates with its corresponding cleaning tank (120). The N filling pumps (330) can be connected to the flushing bucket (310) in the flushing step and connected to at least one of the cleaning buckets (320) in the cleaning step through the control valve group (36), wherein N is an integer greater than or equal to 2.

4. The enzyme-free cleaning system according to claim 1, characterized in that: The invention also comprises a relative motion driving mechanism, wherein the relative motion driving mechanism can cause the cleaning head (110) and the cleaning tank (120) to generate relative motion, and the relative motion driving mechanism comprises a relative motion driving motor (131), wherein the output shaft of the relative motion driving motor (131) is connected to a driving wheel, a transmission wheel (134) is arranged at an interval from the driving wheel, a transmission belt (133) is wound between the driving wheel and the transmission wheel (134), the transmission belt (133) is fixedly connected to a transmission connection block (135), and the transmission connection block (135) is connected to the cleaning head (110) or the cleaning tank (120).

5. The enzyme-free cleaning system according to claim 1, characterized in that: It also includes a lifting drive mechanism. Before or after the cleaning liquid is filled, the cleaning head (110) can be driven by the lifting drive mechanism to lift the tip of the liquid aspiration needle to a position higher than the opening of the ELISA reaction container.

6. The enzyme-free cleaning system according to claim 5, characterized in that: The lifting drive mechanism comprises a lifting motor (141) connected to a lifting base plate, the output end of the lifting motor (141) is connected to a lifting drive gear (142), the lifting drive gear (142) is meshedly connected to a transmission rack (143), the transmission rack (143) is connected to a cleaning head (110), a slide rail (145) is also fixedly connected to the lifting base plate, the cleaning head (110) is directly or indirectly connected to a lifting connection block (144), the lifting connection block (144) is provided with a slide groove, and the lifting connection block (144) is connected to the slide rail (145) by being engaged with the slide groove.

7. The enzyme-free cleaning system according to claim 1, characterized in that: The cleaning head (110) is connected to the lifting base plate via a cleaning head balancing member (146), and two ends of the cleaning head balancing member (146) are respectively connected to the cleaning head (110) and the lifting base plate, and the elastic coefficient of the cleaning head balancing member is 0.13-0.22N / mm.

8. An automated enzyme immunoassay detection device, characterized in that: The enzyme-free cleaning system comprises the enzyme-free cleaning system according to any one of claims 1 to 7, and also comprises an enzyme-free cleaning reaction container for receiving a sample, an oscillating incubation unit and a reaction detection unit, wherein the oscillating incubation unit is capable of incubating and oscillating the enzyme-free cleaning reaction container at a constant temperature when performing an enzyme-linked reaction in the enzyme-free cleaning reaction container, the reaction detection unit is capable of detecting the enzyme-linked reaction in the enzyme-free cleaning reaction container, and the enzyme-free cleaning system is capable of cleaning the enzyme-free cleaning reaction container.

9. A method for cleaning an enzyme-free reaction vessel, characterized in that: The enzyme-free cleaning system according to claim 1 is used, and the enzyme-free cleaning system further includes a relative motion drive mechanism and a lifting drive mechanism, and the method includes: In the cleaning step, the relative motion driving mechanism causes the cleaning head and the cleaning tank to move relative to each other, so that the cleaning head is located above at least part of the ELISA reaction container in the cleaning tank, and the filling pump is connected to one of the cleaning barrels, so that the filling needle can fill the cleaning liquid into the ELISA reaction container; After the cleaning is completed, the lifting drive mechanism drives the cleaning head down until the liquid-absorbing needle approaches or contacts the bottom of the ELISA reaction container. Under the action of the vacuum pump, the waste liquid collection tank is in a negative pressure state. The liquid-absorbing needle discharges the cleaning liquid waste in the ELISA reaction container into the waste liquid collection tank, and the suction port can connect the overflow liquid to the waste liquid collection tank. Before or after the cleaning solution is added, the lifting drive mechanism drives the cleaning head to rise, so that the tip of the aspiration needle is higher than the position of the ELISA reaction container port.

10. The method for cleaning an ELISA reaction container according to claim 9, characterized in that: Also includes a rinse step: The relative motion driving mechanism drives the cleaning head to move above the rinsing position in the cleaning tank, the filling pump is connected to the rinsing barrel, and the injection needle injects the rinsing liquid into the rinsing position. After the rinsing is completed, the waste liquid collection tank is in a negative pressure state under the action of the vacuum pump, and the suction needle sucks the rinsing liquid waste after the rinsing operation into the waste liquid collection tank, wherein the rinsing position is not connected to the cleaning position.

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