Polylactic acid fermentation culture device and method

Abstract

The invention provides a polylactic acid fermentation culture device and method, and belongs to the technical field of polylactic acid.The polylactic acid fermentation culture device comprises a reaction kettle, a liquid inlet and a liquid inlet pipe are arranged on the side portion of the reaction kettle, the liquid inlet is used for adding a sugar raw material and a zymophyte raw material into the reaction kettle, the liquid inlet pipe is used for adding a catalyst into the reaction kettle, and a heater is fixedly connected to the interior of the reaction kettle; the top of the reaction kettle is provided with an air outlet, the upper end of the air outlet is fixedly connected with an air outlet pipe, the lower side of the air outlet pipe is provided with a plurality of groups of shunting mechanisms, the lower side of the air outlet pipe is provided with a support frame, the support frame is detachably connected with a plurality of storage cylinders, and each storage cylinder is connected with each group of shunting mechanism; the lactic acid fermentation process is optimized in a heating and catalyzing mode, the fermentation efficiency of bacterial colonies in the fermentation process is effectively improved, and the quality of polylactic acid is greatly improved.

Description

Technical Field

[0001] The invention belongs to the technical field of polylactic acid, and in particular relates to a polylactic acid fermentation and cultivation device and method. Background Art

[0002] As a biodegradable and environmentally friendly material, polylactic acid has attracted widespread attention due to its unique properties and excellent processability. Polylactic acid can be synthesized through two methods: one is the direct polycondensation of lactic acid monomers, i.e. the direct method; the other is the ring-opening polymerization of lactide, i.e. first synthesizing lactide from lactic acid, and then obtaining polylactic acid through ring-opening polymerization, i.e. the indirect method.

[0003] The prior art discloses a Chinese patent with application number CN202210444741.3, which discloses a polylactic acid fermentation culture device and method, including a fermentation tank for generating lactic acid by fermentation and generating polylactic acid by lactic acid reaction, the top of the fermentation tank is sealed by a sealing cover, and a step heating device is provided on the sealing cover, and a heating chamber is opened inside the tank wall of the fermentation tank, and the step heating device is connected to the inside of the heating chamber through a pipeline and provides heating liquid for the heating chamber in a stepwise manner. The device and method for culturing polylactic acid by microbial fermentation from carbohydrates provided by the present invention optimize the lactic acid fermentation process by step heating, and effectively improve the fermentation efficiency of the colony during the fermentation process. And the step-by-step heating process can make it easier for more types of strains to adapt, so that the polylactic acid produced continuously has a great quality improvement.

[0004] Lactic acid is used as its raw material, which is generally synthesized by fermentation. In the process of lactic acid synthesis, since the fermentation efficiency of each strain at different temperatures is different, and it is difficult to ferment efficiently at high temperature for a long time, in view of this, we propose a polylactic acid fermentation culture device and method Summary of the invention

[0005] The object of the present invention is to provide a polylactic acid fermentation culture device and method, aiming to solve the problems raised in the background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A polylactic acid fermentation and cultivation device comprises a reactor, characterized in that a liquid inlet and a liquid inlet pipe are arranged on the side of the reactor, the liquid inlet is used to add sugar raw materials and fermentation bacteria raw materials into the reactor, the liquid inlet pipe is used to add catalyst into the reactor, a heater is fixedly connected to the interior of the reactor, an air outlet is arranged on the top of the reactor, an air outlet pipe is fixedly connected to the upper end of the air outlet, a plurality of groups of flow distribution mechanisms are arranged on the lower side of the air outlet pipe, a support frame is arranged on the lower side of the air outlet pipe, a plurality of storage cylinders are detachably connected to the support frame, and each of the storage cylinders is connected to each group of flow distribution mechanisms.

[0008] As a preferred solution of the present invention, each group of diversion mechanisms includes a diversion tube, a first valve and a thin tube opening, the diversion tube is fixedly connected to the lower side of the outlet pipe, the first valve is fixedly connected inside the diversion tube, and the thin tube opening is fixedly connected to the bottom of the diversion tube.

[0009] As a preferred solution of the present invention, the top of each storage cylinder is fixedly connected with an air inlet, and the thin tube opening is inserted into the air inlet from top to bottom.

[0010] As a preferred solution of the present invention, a scale is provided on the surface of each storage tube, and a positioning groove is provided on the bottom of each storage tube.

[0011] As a preferred solution of the present invention, a mist outlet pipe is provided in the reactor, one end of the liquid inlet pipe is fixedly connected to the mist outlet pipe, and an atomizer is fixedly connected to the circumferential surface of the liquid inlet pipe.

[0012] As a preferred solution of the present invention, a liquid discharge port is provided at the bottom of the reaction kettle.

[0013] As a preferred solution of the present invention, a plurality of support plates are arranged in the support frame, a positioning block is fixedly connected to the top of each support plate, each positioning block is movably inserted into a corresponding positioning groove, and a group of elastic mechanisms are arranged on the lower side of each support plate.

[0014] As a preferred solution of the present invention, each group of the elastic mechanism includes a telescopic rod and a spring, the telescopic rod is fixedly connected to the lower inner wall of the support frame, the extended end of the telescopic rod is fixedly connected to the bottom of the support plate, and the spring is sleeved on the circumferential surface of the telescopic rod.

[0015] As a preferred solution of the present invention, a plurality of slide grooves are provided on the side of the support frame, and a foot pedal is provided near the slide groove of each support plate, and the foot pedal is slidably connected in the slide groove.

[0016] A method for using a polylactic acid fermentation culture device comprises the following steps:

[0017] S1, adding sugar raw materials and fermentation bacteria raw materials into the reactor through the liquid inlet, and then adding catalyst into the mist outlet pipe through the liquid inlet pipe, the catalyst enters the reactor after atomization, and the pH value of the liquid in the reactor is controlled to be 4.5-5.5 and then the reactor is sealed;

[0018] S2. After 24 hours of catalytic fermentation, the heater is started to heat the reactor several times, with an interval of 1 hour each time, and the heating temperature is controlled at 140-150 degrees;

[0019] S3, after heating for 28 hours, unseal and sterilize by adding steam into the reactor to obtain lactic acid;

[0020] S4. The lactic acid is heated and vaporized to flow upward, and the first valves are opened one by one to allow the lactic acid to enter multiple storage cylinders in sequence. After 10 liters are stored in the storage cylinders, the first valve is closed to complete the polylactic acid cultivation work.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. In this scheme, a catalyst is added into the mist outlet pipe, and the lactic acid fermentation process is optimized by heating and catalysis, which effectively improves the fermentation efficiency of the bacterial colony during the fermentation process and greatly improves the quality of polylactic acid.

[0023] 2. In this scheme, the heated and vaporized lactic acid enters the outlet pipe. When the first valve is opened, the lactic acid enters the diversion pipe and finally enters the storage cylinder from the capillary mouth. The lactic acid cools down and liquefies in the storage cylinder, thereby realizing automatic collection of lactic acid liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0025] In the attached picture:

[0026] Figure 1 A perspective view of the present invention;

[0027] Figure 2 It is a structural diagram of the storage cylinder of the present invention;

[0028] Figure 3 It is a cross-sectional view of a reaction kettle of the present invention;

[0029] Figure 4 It is a structural diagram of the shunt pipe of the present invention;

[0030] Figure 5 It is a structural diagram of the support frame of the present invention;

[0031] Figure 6 is a cross-sectional view of a positioning plate of the present invention;

[0032] Figure 7 It is an exploded view of the spring of the present invention;

[0033] Figure 8 This is a structural diagram of the bottom of the storage cylinder of the present invention.

[0034] Explanation of the numbers in the figure: 1. Reactor; 2. Liquid inlet; 3. Heater; 4. Thermometer; 5. Liquid discharge port; 6. Liquid inlet pipe; 7. Atomizer; 8. Mist outlet pipe; 9. Air outlet; 10. Air outlet pipe; 11. Diverter pipe; 12. First valve; 13. Storage cylinder; 14. Air inlet; 15. Scale; 16. Capillary port; 17. Support frame; 18. Support plate; 19. Telescopic rod; 20. Spring; 21. Positioning block; 22. Positioning groove; 23. Slide groove; 24. Foot pedal. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] Example

[0037] See also Figure 1-Figure 8 , the technical solution provided by this embodiment is as follows:

[0038] A polylactic acid fermentation and cultivation device comprises a reactor 1. A liquid inlet 2 and a liquid inlet pipe 6 are arranged on the side of the reactor 1. The liquid inlet 2 is used to add sugar raw materials and fermentation bacteria raw materials into the reactor 1. The liquid inlet pipe 6 is used to add catalyst into the reactor 1. A heater 3 is fixedly connected to the inside of the reactor 1. An air outlet 9 is arranged on the top of the reactor 1. An air outlet pipe 10 is fixedly connected to the upper end of the air outlet 9. A plurality of groups of flow distribution mechanisms are arranged on the lower side of the air outlet pipe 10. A support frame 17 is arranged on the lower side of the air outlet pipe 10. A plurality of storage cylinders 13 are detachably connected to the support frame 17. Each storage cylinder 13 is connected to each group of flow distribution mechanisms.

[0039] In a specific embodiment of the present invention, the liquid inlet 2 can be used to add sugar raw materials and fermentation bacteria raw materials into the reactor 1, the soup raw material can use glucose stock solution, the fermentation bacteria can be any one of Lactobacillus acidophilus and Lactobacillus delbrueckii, and the liquid inlet pipe 6 is used to add a catalyst into the reactor 1. When in use, the sugar raw material and fermentation bacteria raw material are added to the reactor 1 through the liquid inlet 2, and then the catalyst is added to the mist outlet pipe 8 through the liquid inlet pipe 6. The catalyst enters the reactor 1 after atomization, and the pH value of the liquid in the reactor 1 is controlled to be 4.5-5.5, and then the reactor 1 is sealed. After catalytic fermentation for 24 hours, the heater 3 is started to heat the reactor 1 multiple times, each time at an interval of 1 hour. Heating, controlling the heating temperature at 140 degrees to 150 degrees, after heating for 28 hours, removing the seal, and adding steam to the reactor 1 for sterilization to obtain lactic acid, the lactic acid is heated and vaporized and flows upward, and the first valves 12 are opened one by one to allow the lactic acid to enter multiple storage cylinders 13 in sequence. After 10 liters are stored in the storage cylinder 13, the first valve 12 is closed to complete the cultivation of polylactic acid. The lactic acid fermentation process is optimized by heating and catalysis, which effectively improves the fermentation efficiency of the colony during the fermentation process, and greatly improves the quality of polylactic acid. Preferably, a thermometer 4 is fixedly connected to the surface of the reactor 1 to monitor the internal temperature of the reactor 1 in real time.

[0040] Specifically, each group of diversion mechanisms includes a shunt tube 11, a first valve 12 and a capillary port 16. The shunt tube 11 is fixedly connected to the lower side of the outlet pipe 10, the first valve 12 is fixedly connected inside the shunt tube 11, the capillary port 16 is fixedly connected to the bottom of the shunt tube 11, and the top of each storage tube 13 is fixedly connected to an air inlet 14, and the capillary port 16 is inserted into the air inlet 14 from top to bottom.

[0041] In a specific embodiment of the present invention, the heated and vaporized lactic acid enters the outlet pipe 10. When the first valve 12 is opened, the lactic acid enters the diversion pipe 11 and finally enters the storage tube 13 through the capillary opening 16. The lactic acid is cooled and liquefied in the storage tube 13, thereby realizing automatic collection of the lactic acid liquid.

[0042] Specifically, a scale 15 is disposed on the surface of each storage tube 13 , and a positioning groove 22 is disposed on the bottom of each storage tube 13 .

[0043] In a specific embodiment of the present invention, the storage tube 13 is made of transparent plastic material, and the amount of lactic acid stored in the storage tube 13 can be directly observed under the display of the scale 15 .

[0044] Specifically, a mist outlet pipe 8 is provided in the reactor 1 , one end of the liquid inlet pipe 6 is fixedly connected to the mist outlet pipe 8 , and an atomizer 7 is fixedly connected to the circumferential surface of the liquid inlet pipe 6 .

[0045] In a specific embodiment of the present invention, refer to Figure 3The catalyst is atomized by the atomizer 7 and enters the atomization pipe 8, and then enters the reactor 1 evenly, so that it can be mixed with the sugar raw material more quickly and the catalytic speed can be accelerated.

[0046] Specifically, a drain port 5 is provided at the bottom of the reactor 1 .

[0047] In a specific embodiment of the present invention, the drain port 5 is used to discharge the liquid in the reaction kettle 1 to facilitate subsequent cleaning work.

[0048] Specifically, a plurality of support plates 18 are arranged in the support frame 17, a positioning block 21 is fixedly connected to the top of each support plate 18, each positioning block 21 is movably inserted into the corresponding positioning groove 22, and a group of elastic mechanisms are arranged on the lower side of each support plate 18, each group of elastic mechanisms includes a telescopic rod 19 and a spring 20, the telescopic rod 19 is fixedly connected to the lower inner wall of the support frame 17, the extended end of the telescopic rod 19 is fixedly connected to the bottom of the support plate 18, and the spring 20 is sleeved on the circumferential surface of the telescopic rod 19.

[0049] In a specific embodiment of the present invention, the positioning block 21 corresponds to the position of the positioning groove 22, the support plate 18 fits the bottom of the storage tube 13, and the telescopic rod 19 is used to limit the deformation of the spring 20 to prevent the spring 20 from tilting. During installation, the positioning block 21 is inserted into the positioning groove 22. Under the elastic action of the spring 20, the support plate 18 pushes the storage tube 13 to move upward, so that the capillary 16 is inserted into the air inlet 14 without separation, and the position of the storage tube 13 is automatically fixed.

[0050] Specifically, a plurality of slide grooves 23 are provided on the side of the support frame 17 , and a foot pedal 24 is provided near the slide groove 23 on each support plate 18 , and the foot pedal 24 is slidably connected in the slide groove 23 .

[0051] In a specific embodiment of the present invention, the slide groove 23 is used to limit the movement of the foot pedal 24 to ensure the stable lifting and lowering of the support plate 18. The foot pedal 24 is stepped on, which is more labor-saving.

[0052] A method for using a polylactic acid fermentation culture device comprises the following steps:

[0053] S1, adding sugar raw materials and fermentation bacteria raw materials into the reactor 1 through the liquid inlet 2, and then adding catalyst into the mist outlet pipe 8 through the liquid inlet pipe 6, the catalyst enters the reactor 1 after atomization, and the pH value of the liquid in the reactor 1 is controlled to be 4.5-5.5, and then the reactor 1 is sealed;

[0054] S2, after 24 hours of catalytic fermentation, start the heater 3 to heat the reactor 1 multiple times, each heating interval is 1 hour, and the heating temperature is controlled at 140 degrees to 150 degrees;

[0055] S3, after heating for 28 hours, unseal and sterilize by adding steam into the reactor 1 to obtain lactic acid;

[0056] S4, the lactic acid is heated and vaporized to flow upward, and the first valves 12 are opened one by one to allow the lactic acid to enter the multiple storage cylinders 13 in sequence. After 10 liters of lactic acid is stored in the storage cylinders 13, the first valve 12 is closed to complete the polylactic acid cultivation work.

[0057] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A polylactic acid fermentation and culture device, comprising a reaction kettle (1), characterized in that: The reactor (1) is provided with a liquid inlet (2) and a liquid inlet pipe (6) on the side thereof, wherein the liquid inlet (2) is used for adding sugar raw materials and fermentation bacteria raw materials into the reactor (1), and the liquid inlet pipe (6) is used for adding catalyst into the reactor (1). The reactor (1) is fixedly connected with a heater (3) inside, and the reactor (1) is provided with an air outlet (9) on the top thereof, and an air outlet pipe (10) is fixedly connected to the upper end of the air outlet (9), and a plurality of flow distribution mechanisms are provided on the lower side of the air outlet pipe (10), and a support frame (17) is provided on the lower side of the air outlet pipe (10), and a plurality of storage cylinders (13) are detachably connected to the support frame (17), and each storage cylinder (13) is connected with each group of flow distribution mechanisms.

2. A polylactic acid fermentation and culture device according to claim 1, characterized in that: Each group of diversion mechanisms comprises a diversion pipe (11), a first valve (12) and a thin tube opening (16); the diversion pipe (11) is fixedly connected to the lower side of the air outlet pipe (10); the first valve (12) is fixedly connected inside the diversion pipe (11); and the thin tube opening (16) is fixedly connected to the bottom of the diversion pipe (11).

3. A polylactic acid fermentation and culture device according to claim 2, characterized in that: The top of each storage cylinder (13) is fixedly connected to an air inlet (14), and the thin tube opening (16) is inserted into the air inlet (14) from top to bottom.

4. A polylactic acid fermentation and culture device according to claim 3, characterized in that: The surface of each storage tube (13) is provided with a scale (15), and the bottom of each storage tube (13) is provided with a positioning groove (22).

5. A polylactic acid fermentation and culture device according to claim 4, characterized in that: A mist outlet pipe (8) is arranged in the reaction kettle (1), one end of the liquid inlet pipe (6) is fixedly connected to the mist outlet pipe (8), and an atomizer (7) is fixedly connected to the circumferential surface of the liquid inlet pipe (6).

6. A polylactic acid fermentation and culture device according to claim 5, characterized in that: The bottom of the reaction kettle (1) is provided with a liquid discharge port (5).

7. A polylactic acid fermentation and culture device according to claim 6, characterized in that: A plurality of support plates (18) are arranged in the support frame (17), a positioning block (21) is fixedly connected to the top of each support plate (18), each positioning block (21) is movably inserted into a corresponding positioning groove (22), and a group of elastic mechanisms are arranged on the lower side of each support plate (18).

8. A polylactic acid fermentation and culture device according to claim 7, characterized in that: Each group of the elastic mechanisms comprises a telescopic rod (19) and a spring (20); the telescopic rod (19) is fixedly connected to the lower inner wall of the support frame (17); the extended end of the telescopic rod (19) is fixedly connected to the bottom of the support plate (18); and the spring (20) is sleeved on the circumferential surface of the telescopic rod (19).

9. A polylactic acid fermentation and culture device according to claim 8, characterized in that: A plurality of slide grooves (23) are provided on the side of the support frame (17), and a foot pedal (24) is provided near the slide groove (23) on each support plate (18), and the foot pedal (24) is slidably connected in the slide groove (23).

10. A method for using a polylactic acid fermentation culture device, characterized in that: The steps include: S1, adding sugar raw material and fermentation bacteria raw material into the reactor (1) through the liquid inlet (2), then adding catalyst into the mist outlet pipe (8) through the liquid inlet pipe (6), the catalyst enters the reactor (1) after being atomized, and the pH value of the liquid in the reactor (1) is controlled to be 4.5-5.5, and then the reactor (1) is sealed; S2, after 24 hours of catalytic fermentation, start the heater (3) to heat the reactor (1) multiple times, each heating interval is 1 hour, and the heating temperature is controlled at 140 degrees to 150 degrees; S3, after heating for 28 hours, unseal and add steam into the reaction kettle (1) for sterilization to obtain lactic acid; S4, the lactic acid is heated and vaporized to flow upward, and the first valves (12) are opened one by one, so that the lactic acid enters into a plurality of storage cylinders (13) in sequence. After 10 liters are stored in the storage cylinders (13), the first valve (12) is closed, and the polylactic acid cultivation work is completed.

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