Optimal parameter simulation method and preparation device for preparation of chromosome sample slide

Abstract

The invention discloses an optimal parameter simulation method for preparation of a chromosome sample slide and a preparation device, and belongs to the technical field of chromosome preparation. According to the preparation optimal parameter simulation method of the chromosome sample slide, the preparation parameters in the preparation process of the chromosome sample slide are adjusted by constructing the preparation evaluation model, the effect judgment model and the parameter adjustment model, so that the preparation optimal parameter simulation of the chromosome sample slide is realized; parameters of all links are optimized through a model algorithm, the working efficiency of a cytogenetics laboratory is greatly improved, the calculation result is more reliable, the influence of human intervention and subjective factors is effectively reduced, and then chromosome preparation with higher quality can be achieved. According to the preparation device, the whole chromosome preparation process can be automated, seamless connection of all links can be achieved, the slide preparation quality can be monitored and adjusted in real time, and the working efficiency and operation reliability of a laboratory are remarkably improved.

Description

Technical Field

[0001] The invention relates to a method and a device for simulating optimal parameters for preparing a chromosome sample slide, and belongs to the technical field of chromosome preparation. Background Art

[0002] The final effect of chromosome preparation needs to be judged by manual microscopic examination. The chromosome preparation process involves three links: culture, harvesting, and slide preparation (staining). Specifically, for the final effect of slide preparation, the current general practice is for professional pre-treatment process personnel to randomly select a slide from the currently prepared batch of slide samples, and use a biological microscope to observe the number of cells, the number and dispersion of mitotic phases, and staining, so as to judge the quality of cell culture harvest and the quality of slide preparation and staining.

[0003] With the development of laboratory automation equipment, chromosome preparation has gradually changed from early manual single operations to manual operation of single-function equipment to achieve a complete process. However, the following problems still exist:

[0004] 1. The preparation process is highly dependent on manual experience, and parameter adjustment is complex and time-consuming.

[0005] The performance of equipment and reagents from different manufacturers varies greatly, and manual exploration is required to improve the production effect, which is time-consuming and laborious. In the cytogenetics laboratory, "probing" is a critical and complex step that directly affects the quality of chromosome preparation.

[0006] Since the operation process is more dependent on manual labor and the factors that affect the final sample preparation effect are more complex, operators need to find the best parameters through repeated experiments and experience accumulation, which greatly increases the time and labor costs of the laboratory. In addition, even if the best parameters are found, due to differences between batches and differences in human operations, they still need to be constantly fine-tuned, which further increases the workload and complexity.

[0007] 2. The process is complicated and lacks an overall automation solution.

[0008] The entire process is relatively complicated. There is currently no equipment on the market that automates the entire process. Most of the equipment in each link exists separately, such as the independent inoculation equipment, incubators, harvesting instruments, slicing equipment, ovens and staining instruments that are commonly seen on the market.

[0009] 3. The equipment module has low integration, requiring frequent manual intervention, increasing the risk of contamination and errors.

[0010] The equipment module has a low integration level and cannot run through the entire process automatically. It requires manual intervention after each process is completed and operates the next process to finally complete the entire sample processing. In addition, manual microscopy is still required to judge the final preparation effect.

[0011] This decentralized equipment configuration and manual operation intervention not only increases the workload of the laboratory, but may also lead to problems such as inconsistent results and poor repeatability. Manual transfer and operation between each link increases the risk of sample contamination and errors, and also prolongs the time of the entire preparation process. In addition, due to the lack of real-time monitoring and automatic adjustment mechanisms, the quality control of the entire process mainly depends on the experience and judgment of the operator, which may lead to batch-to-batch differences and instability.

[0012] The information disclosed in this Background Art is only for understanding the background of the inventive concept and therefore it may include information that does not constitute the prior art. Summary of the invention

[0013] In response to the above problem or one of the above problems, an object of the present invention is to provide a method for simulating optimal parameters for the preparation of chromosome sample slides. By constructing a production evaluation model, an effect judgment model, and a parameter adjustment model, the production parameters in the process of chromosome sample slide preparation are adjusted to achieve the simulation of optimal parameters for the preparation of chromosome sample slides. By optimizing the parameters of each link through the model algorithm, the work efficiency of the cytogenetic laboratory will be greatly improved, the calculation results will be more reliable, and the consistency of parameter settings can be ensured, which can effectively reduce human intervention and the influence of subjective factors, thereby achieving higher quality chromosome preparation.

[0014] In response to the above problem or one of the above problems, the second object of the present invention is to provide a device for preparing chromosome sample slides. By setting up an inoculation culture instrument, an automatic cell harvester, an all-in-one slide making and staining machine, an automatic cell microscopic scanning system and a control system, the entire chromosome preparation process can be automated, each link can be seamlessly connected, and the preparation quality can be monitored and adjusted in real time, which significantly improves the laboratory's work efficiency and operational reliability.

[0015] To achieve one of the above purposes, the first technical solution of the present invention is:

[0016] A method for simulating optimal parameters for preparing a chromosome sample slide comprises the following steps:

[0017] Step 1, obtaining the slide preparation parameters of a chromosome sample during the slide preparation process;

[0018] Step 2: Scan and evaluate the chromosome sample slide obtained based on the slide production parameters using a pre-built slide production evaluation model to obtain a slide evaluation result;

[0019] Step 3: using the pre-built effect judgment model, judging whether the slide production effect meets the standard according to the slide evaluation result, if the slide production effect meets the standard, outputting the current slide production parameters and executing step 6;

[0020] If the production effect does not meet the standard, proceed to step 4;

[0021] Step 4: using the pre-built parameter adjustment model, based on the slide making effect, adjusting the slide making parameters in the chromosome sample slide preparation process, determining new slide making parameters, and executing step 2;

[0022] Step five, using the current slide making parameters as the optimal parameters for batch slide making, and using them for batch slide making to achieve the optimal parameter simulation for the preparation of chromosome sample slides.

[0023] After continuous exploration and experiments, the present invention adjusts the preparation parameters in the process of chromosome sample slide preparation by constructing a preparation evaluation model, an effect judgment model, and a parameter adjustment model, thereby realizing the simulation of the optimal preparation parameters of chromosome sample slides; by optimizing the parameters of each link through the model algorithm, the work efficiency of the cytogenetics laboratory will be greatly improved, the calculation results will be more reliable, and the consistency of parameter settings can be ensured, which effectively reduces the influence of human intervention and subjective factors, thereby achieving higher quality chromosome preparation.

[0024] As the preferred technical measures:

[0025] Step 2: Scan and evaluate the chromosome sample slides obtained based on the slide production parameters using a pre-built slide production evaluation model to obtain slide evaluation results;

[0026] Obtaining the slide preparation parameters, including incubation temperature, incubation time, centrifugation speed and time, drop height and angle, digestion time and staining time;

[0027] Based on the production parameters, set up the chromosome preparation device;

[0028] Obtaining a chromosome sample slide through the set-up chromosome preparation device;

[0029] The chromosome sample slide is transferred to the scanning system for scanning and analysis, and the number and quality of the chromosome metaphase division phases on the chromosome sample slide are detected. The number and quality of the chromosome metaphase division phases at least include the number of metaphase division phases, chromosome length, dispersion and banding staining conditions;

[0030] The chromosome length, dispersion and banding staining are coupled to form the slide evaluation results.

[0031] As the preferred technical measures:

[0032] Step 3: Using the pre-built effect judgment model, the method for judging whether the slide production effect meets the standard according to the slide evaluation results is as follows:

[0033] Obtaining film production evaluation standards, which at least include the standard number of metaphase divisions, standard chromosome length, standard dispersion, and standard banding quality;

[0034] Compare the slide evaluation criteria with the slide evaluation results to analyze whether the number of metaphases, chromosome length, dispersion, and banding quality in the chromosome sample slides meet the standards;

[0035] If the number of metaphases, chromosome length and dispersion effect in the chromosome sample slide exceed the slide preparation evaluation standard, it means that the slide preparation effect meets the standard, and the slide preparation parameters at this time are output;

[0036] If the number of metaphases, chromosome length and dispersion effect in the chromosome sample slide do not meet the production evaluation standards, it means that the production effect is not up to standard, that is, the number of metaphases is small, the chromosomes are too short, the clustering and crossing are serious, or the banding is not clear.

[0037] As the preferred technical measures:

[0038] Step 4: Using the pre-built parameter adjustment model, the method for adjusting the slide preparation parameters in the chromosome sample slide preparation process based on the slide preparation effect is as follows:

[0039] Adjust the incubation time and temperature, including the following:

[0040] If the number of mitotic phases is insufficient, extend the cell culture time or fine-tune the culture temperature to promote cell proliferation; extend the culture time to increase the number of mitotic phases, and adjust the temperature to optimize cell growth conditions;

[0041] Adjust the centrifugation speed and time, including the following:

[0042] Centrifugation conditions will affect the sedimentation state of cells; if cells are unevenly distributed or aggregated, reduce the centrifugation speed or shorten the centrifugation time to ensure that the cells are evenly distributed on the chromosome sample slide;

[0043] Adjust the drop sheet height and angle, which includes the following:

[0044] If the chromosome dispersion is not ideal, optimize the distribution of cells on the chromosome sample slide by adjusting the drop height or angle; adjusting the drop height improves cell rupture, while adjusting the angle helps cells spread evenly;

[0045] Adjust digestion time, which includes the following:

[0046] For cases of poor chromosome dispersion and aggregation, the digestion time should be extended to ensure effective chromosome separation; if the chromosomes are excessively banded, the digestion time should be shortened;

[0047] Adjust the staining time and conditions, including the following:

[0048] If the band is not clear, extend the staining time; if the staining is too dark, shorten the staining time; at the same time, check and adjust the temperature and humidity in the staining environment to ensure the best reaction effect of the stain.

[0049] To achieve one of the above purposes, the second technical solution of the present invention is:

[0050] A method for simulating optimal parameters for preparing a chromosome sample slide, comprising the following contents:

[0051] Through the pre-built slide evaluation model, the chromosome sample slide is scanned and evaluated to obtain the slide evaluation result;

[0052] Use the pre-built effect judgment model to judge whether the slide production effect meets the standard based on the slide evaluation results;

[0053] If the slide preparation effect does not meet the standard, the pre-built parameter adjustment model is used to adjust the equipment parameters in the chromosome sample slide preparation process based on the slide preparation effect to obtain new slide preparation parameters;

[0054] Use the pre-built re-slide model to obtain new chromosome sample slides based on new slide making parameters, and re-scan and evaluate the slide making results;

[0055] If the production effect is up to standard, the production parameters at this time are output as the optimal parameters for batch production, which are used for batch production to ensure the consistency of the quality of chromosome samples in each batch and realize the simulation of the optimal parameters for the preparation of chromosome sample slides.

[0056] To achieve one of the above purposes, the third technical solution of the present invention is:

[0057] A chromosome sample slide preparation device, comprising an inoculation culture instrument, an automatic cell harvester, a slide preparation and staining all-in-one machine, an automatic cell microscopic scanning system and a control system;

[0058] Inoculation and culture instrument, used to complete the inoculation and culture of chromosome samples;

[0059] An automatic cell harvester, used to receive culture medium from an inoculated culture instrument and perform a series of processing operations;

[0060] The slide making and staining machine is used to make the processed chromosome samples on the glass slides and digest and stain them to obtain the sample slides that can be used for chromosome scanning and analysis;

[0061] An automated cell microscopy scanning system for scanning and image capture of stained slides for chromosome karyotype analysis;

[0062] The control system includes a central processing unit, a sensor network, a data processing module, and a safety monitoring module, which is used to coordinate the operation of the inoculation culture instrument, the automatic cell harvester, the slide preparation and staining machine, and the automatic cell microscopy scanning system to ensure the operation of the entire process and the unified management of data.

[0063] The present invention automates the entire chromosome preparation process by providing an inoculation culture instrument, an automatic cell harvester, an integrated preparation and staining machine, an automatic cell microscopic scanning system and a control system, thereby achieving seamless connection of various links, and being able to monitor and adjust the preparation quality in real time, thereby significantly improving the work efficiency and operational reliability of the laboratory.

[0064] As the preferred technical measures:

[0065] Inoculation culture apparatus, including a robotic arm, an incubator, and a conveyor belt;

[0066] The incubator is equipped with temperature and humidity sensors and culture medium;

[0067] The blood sample is inoculated on the culture medium through the built-in robotic arm to complete the inoculation of chromosome samples and ensure aseptic operation;

[0068] The temperature and humidity sensors can collect the temperature and humidity in the incubator to adjust the environmental conditions in the incubator and maintain appropriate culture temperature and humidity;

[0069] After the culture is completed, the culture medium is transferred to the automatic cell harvester by a conveyor belt;

[0070] Or / and, an automatic cell harvester for receiving culture medium from an inoculation culture instrument and performing a series of processing operations as follows:

[0071] For chromosome samples in the culture medium, add an appropriate amount of colchicine to block cell division;

[0072] The chromosome samples were centrifuged to separate the cell pellet and the supernatant;

[0073] Aspirate the chromosome sample to discard the supernatant and retain the cell pellet;

[0074] Add hypotonic solution to the chromosome sample and swell the cells by shaking;

[0075] Add fixative to the chromosome sample to fix the cell morphology;

[0076] Perform a second centrifugation on the chromosome sample and discard the supernatant to ensure a pure cell suspension, providing better materials for subsequent chromosome preparation and analysis;

[0077] After the chromosome sample is harvested, the chromosome sample is transferred to the slide preparation and staining machine.

[0078] As the preferred technical measures:

[0079] The slide making and staining machine includes a liquid transfer device, a slide baking device, a manipulator, a digestion tank and a staining tank;

[0080] Use a pipette to drop the treated cell suspension onto a glass slide, and spread the cells on the glass slide to form a thin layer for easy observation under a microscope;

[0081] The baking device is used to adjust the temperature. The prepared slides are placed in the preheated baking device. The chromosome samples are dried by baking at a constant temperature to quickly fix the cells and ensure that the morphology and structure of the cells remain stable during the preparation process.

[0082] The frosted area of ​​the slide is grabbed by a robot, and the chromosome sample is placed in a digestion tank. The cells are treated with trypsin to decompose the proteins between cells, separate the cells, and remove the connecting substances between cells, so that the chromosomes can be dispersed for easy observation and analysis;

[0083] Wash the digested slides with buffer to remove residual enzymes, culture medium components or impurities, prevent background interference during staining, and ensure the clarity of chromosomes;

[0084] The frosted area of ​​the slide is grabbed by a robot, and the chromosome sample is immersed in a staining jar. The chromosome is stained with a specific dye to make the chromosome banding characteristics visible and the chromosome structure clear, which is convenient for chromosome analysis and identification.

[0085] Transfer the prepared slides to the automated cell microscopy scanning system;

[0086] or / and, an automatic cell microscopic scanning system, comprising a film retrieval component, a stage, an automatic microscopic imaging system and a storage component;

[0087] The slide retrieval assembly pulls the slide to the stage, and the stage locates the slide scanning area;

[0088] The automatic microscopic imaging system includes a fully automatic stage, an automatic focusing system, optical imaging and a high-definition camera device, which can perform a full-scale scan of the slide scanning area to obtain high-definition cell images;

[0089] The cell images scanned by the storage component are convenient for chromosome karyotype analysis and archiving.

[0090] As the preferred technical measures:

[0091] The control system coordinates the work of the inoculation culture instrument, cell harvester, slide preparation and staining machine, and automatic cell microscopy scanning system in real time through the central processing unit and embedded control program to ensure seamless connection according to the predetermined process flow;

[0092] The control system integrates a sensor network, which includes at least a temperature sensor, a humidity sensor, a pressure sensor, a liquid level sensor and a position sensor, for real-time monitoring of the status and process parameters of each module;

[0093] Through the feedback data from these sensors, the control system can dynamically adjust the operation of each submodule; it includes the following:

[0094] When temperature deviation is detected during the culture process, the temperature in the incubator is adjusted; during the preparation process, if the drop height or cell distribution is abnormal, it is corrected by adjusting the movement of the robotic arm; this feedback adjustment mechanism ensures the accuracy and consistency of chromosome sample processing;

[0095] The control system integrates a data processing module to automatically record and track every step of the chromosome sample operation to ensure the uniqueness and traceability of the information. First, the chromosome sample is identified and encoded to ensure the uniqueness of the chromosome sample information. Then the processing status of the chromosome sample in each module is tracked, including culture time, harvesting conditions, preparation and staining parameters, to ensure the traceability of the entire process.

[0096] The control system uses a built-in safety monitoring module to detect the operating status of the equipment in real time to ensure safety during the working process; the sensor network monitors key parameters in real time, including temperature, pressure and liquid level. When an abnormality occurs, it will alarm and shut down to prevent damage to chromosome samples or expansion of equipment failures; the control system has automatic shutdown and fault self-diagnosis functions, which can save current data when a fault occurs, generate a fault report, and prompt operators to perform maintenance to ensure the continuity and safety of the work process.

[0097] To achieve one of the above purposes, the fourth technical solution of the present invention is:

[0098] A computer-readable storage medium stores a computer program, which, when executed by a processor, implements the above-mentioned optimal parameter simulation method for preparing a chromosome sample slide.

[0099] Compared with the prior art solutions, the present invention has the following beneficial effects:

[0100] After continuous exploration and experiments, the present invention adjusts the preparation parameters in the process of chromosome sample slide preparation by constructing a preparation evaluation model, an effect judgment model, and a parameter adjustment model, thereby realizing the simulation of the optimal preparation parameters of chromosome sample slides; by optimizing the parameters of each link through the model algorithm, the work efficiency of the cytogenetics laboratory will be greatly improved, the calculation results will be more reliable, and the consistency of parameter settings can be ensured, which effectively reduces the influence of human intervention and subjective factors, thereby achieving higher quality chromosome preparation.

[0101] Furthermore, the present invention can automate the entire chromosome preparation process by providing an inoculation culture instrument, an automatic cell harvester, an integrated preparation and staining machine, an automatic cell microscopic scanning system and a control system, thereby achieving seamless connection of each link, and being able to monitor and adjust the preparation quality in real time, thereby significantly improving the laboratory's work efficiency and operational reliability.

[0102] Furthermore, the present invention integrates multiple functional modules such as inoculation, cell harvesting, slide preparation, staining, and microscopic scanning into the same system. Each functional module is seamlessly connected through an automated transmission system, avoiding manual transfer operations between multiple devices. This highly integrated automated design greatly reduces manual intervention, reduces the risk of contamination and operational errors when samples are transferred between different devices, and significantly improves operational convenience and the consistency and stability of sample processing.

[0103] Furthermore, the present invention can evaluate key indicators on the slide in real time (such as the number of metaphase divisions, chromosome dispersion and band clarity) through an automatic scanning system. If the slide production effect does not meet the standard, the system can automatically adjust the process parameters such as culture time, centrifugation conditions, drop height, digestion and staining time, and re-produce the slide until the best slide production effect is obtained. This innovative automatic adjustment mechanism ensures the quality consistency of each batch of slide production, greatly reduces the error of human adjustment, and improves the reliability and stability of slide production.

[0104] Furthermore, the present invention realizes the automation of the whole process from sample inoculation, cell harvesting to slide making and staining, reducing the complexity of manual operation. After optimizing the parameters, the system can perform batch slide making operations to ensure the consistency and efficiency of large-scale sample processing. This innovative automation design greatly improves the sample processing capacity of the laboratory, enabling the laboratory to process a large number of samples in a short time, significantly improving work efficiency and meeting the needs of high-throughput experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0105] Figure 1 A schematic diagram of a process for preparing an optimal parameter simulation method of the present invention;

[0106] Figure 2A schematic diagram of a process for preparing a chromosome sample slide according to the present invention;

[0107] Figure 3 A schematic diagram of the preparation device of the present invention. DETAILED DESCRIPTION

[0108] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0109] On the contrary, the present invention covers any substitution, modification, equivalent method and scheme made on the essence and scope of the present invention as defined by the claims. Further, in order to make the public have a better understanding of the present invention, some specific details are described in detail in the following detailed description of the present invention. Those skilled in the art can fully understand the present invention without the description of these details.

[0110] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which the present invention pertains. The terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "or / and" as used herein includes any and all combinations of one or more of the associated listed items.

[0111] like Figure 1 As shown, the first specific embodiment of the optimal parameter simulation method for preparing chromosome sample slides of the present invention is as follows:

[0112] A method for simulating optimal parameters for preparing a chromosome sample slide comprises the following steps:

[0113] Step 1, obtaining the slide preparation parameters of a chromosome sample during the slide preparation process;

[0114] Step 2: Scan and evaluate the chromosome sample slides obtained based on the slide production parameters using a pre-built slide production evaluation model to obtain slide evaluation results;

[0115] Step 3: using the pre-built effect judgment model, judging whether the slide production effect meets the standard according to the slide evaluation result, if the slide production effect meets the standard, outputting the current slide production parameters and executing step 6;

[0116] If the production effect does not meet the standard, proceed to step 4;

[0117] Step 4: using the pre-built parameter adjustment model, based on the slide making effect, adjusting the slide making parameters in the chromosome sample slide preparation process, determining new slide making parameters, and executing step 2;

[0118] Step five, using the current slide making parameters as the optimal parameters for batch slide making, and using them for batch slide making to achieve the optimal parameter simulation for the preparation of chromosome sample slides.

[0119] In this embodiment: Step 2, a pre-constructed slide evaluation model is used to scan and evaluate the chromosome sample slide obtained based on the slide production parameters to obtain a slide evaluation result;

[0120] Obtaining the slide preparation parameters, including incubation temperature, incubation time, centrifugation speed and time, drop height and angle, digestion time and staining time;

[0121] Based on the production parameters, set up the chromosome preparation device;

[0122] Obtaining a chromosome sample slide through the set-up chromosome preparation device;

[0123] The chromosome sample slide is transferred to the scanning system for scanning and analysis, and the number and quality of the chromosome metaphase division phases on the chromosome sample slide are detected. The number and quality of the chromosome metaphase division phases at least include the number of metaphase division phases, chromosome length, dispersion and banding staining conditions;

[0124] The chromosome length, dispersion and banding staining are coupled to form the slide evaluation results.

[0125] In this embodiment: Step 3, using the pre-built effect judgment model, the method for judging whether the slide production effect meets the standard according to the slide evaluation result is as follows:

[0126] Obtaining film production evaluation standards, which at least include the standard number of metaphase divisions, standard chromosome length, standard dispersion, and standard banding quality;

[0127] Compare the slide evaluation criteria with the slide evaluation results to analyze whether the number of metaphases, chromosome length, dispersion, and banding quality in the chromosome sample slides meet the standards;

[0128] If the number of metaphases, chromosome length and dispersion effect in the chromosome sample slide exceed the slide preparation evaluation standard, it means that the slide preparation effect meets the standard, and the slide preparation parameters at this time are output;

[0129] If the number of metaphases, chromosome length and dispersion effect in the chromosome sample slide do not meet the production evaluation standards, it means that the production effect is not up to standard, that is, the number of metaphases is small, the chromosomes are too short, the clustering and crossing are serious, or the banding is not clear.

[0130] In this embodiment: Step 4, the method of adjusting the slide preparation parameters in the chromosome sample slide preparation process based on the slide preparation effect using the pre-built parameter adjustment model is as follows:

[0131] Adjust the incubation time and temperature, including the following:

[0132] If the number of mitotic phases is insufficient, extend the cell culture time or fine-tune the culture temperature to promote cell proliferation; extend the culture time to increase the number of mitotic phases, and adjust the temperature to optimize cell growth conditions;

[0133] Adjust the centrifugation speed and time, including the following:

[0134] Centrifugation conditions will affect the sedimentation state of cells; if cells are unevenly distributed or aggregated, reduce the centrifugation speed or shorten the centrifugation time to ensure that the cells are evenly distributed on the chromosome sample slide;

[0135] Adjust the drop sheet height and angle, which includes the following:

[0136] If the chromosome dispersion is not ideal, optimize the distribution of cells on the chromosome sample slide by adjusting the drop height or angle; adjusting the drop height improves cell rupture, while adjusting the angle helps cells spread evenly;

[0137] Adjust digestion time, which includes the following:

[0138] For cases of poor chromosome dispersion and aggregation, the digestion time should be extended to ensure effective chromosome separation; if the chromosomes are excessively banded, the digestion time should be shortened;

[0139] Adjust the staining time and conditions, including the following:

[0140] If the band is not clear, extend the staining time; if the staining is too dark, shorten the staining time; at the same time, check and adjust the temperature and humidity in the staining environment to ensure the best reaction effect of the stain.

[0141] The second specific embodiment of the optimal parameter simulation method for preparing chromosome sample slides of the present invention:

[0142] A method for simulating optimal parameters for preparing a chromosome sample slide, comprising the following contents:

[0143] Through the pre-built slide evaluation model, the chromosome sample slide is scanned and evaluated to obtain the slide evaluation result;

[0144] Use the pre-built effect judgment model to judge whether the slide production effect meets the standard based on the slide evaluation results;

[0145] If the slide preparation effect does not meet the standard, the pre-built parameter adjustment model is used to adjust the equipment parameters in the chromosome sample slide preparation process based on the slide preparation effect to obtain new slide preparation parameters;

[0146] Use the pre-built re-slide model to obtain new chromosome sample slides based on new slide making parameters, and re-scan and evaluate the slide making results;

[0147] If the production effect is up to standard, the production parameters at this time are output as the optimal parameters for batch production, which are used for batch production to ensure the consistency of the quality of chromosome samples in each batch and realize the simulation of the optimal parameters for the preparation of chromosome sample slides.

[0148] The first specific embodiment of the chromosome sample slide preparation device of the present invention:

[0149] A chromosome sample slide preparation device, comprising an inoculation culture instrument, an automatic cell harvester, a slide preparation and staining all-in-one machine, an automatic cell microscopic scanning system and a control system;

[0150] Inoculation and culture instrument, used to complete the inoculation and culture of chromosome samples;

[0151] An automatic cell harvester, used to receive culture medium from an inoculated culture instrument and perform a series of processing operations;

[0152] The slide making and staining machine is used to make the processed chromosome samples on the glass slides and digest and stain them to obtain the sample slides that can be used for chromosome scanning and analysis;

[0153] An automated cell microscopy scanning system for scanning and image capture of stained slides for chromosome karyotype analysis;

[0154] The control system includes a central processing unit, a sensor network, a data processing module, and a safety monitoring module, which is used to coordinate the operation of the inoculation culture instrument, the automatic cell harvester, the slide preparation and staining machine, and the automatic cell microscopy scanning system to ensure the operation of the entire process and the unified management of data.

[0155] Inoculation culture instrument: used for inoculation and culture of samples. The system inoculates sample blood into the culture medium through a robotic arm and adjusts the temperature and humidity for culture.

[0156] Automatic cell harvester: responsible for receiving samples after culture, and processing and collecting cell precipitates through automated steps such as adding colchicine, centrifugation, discarding supernatant, adding hypotonic solution, and adding fixative.

[0157] All-in-one slide preparation and staining machine: drop the cell suspension onto the slide, perform baking, trypsin digestion, staining and other operations to prepare cell slides for microscopic analysis.

[0158] Automatic cell microscopic scanning system: Microscopically scan the stained slides to obtain high-definition chromosome images, which are automatically stored for subsequent karyotype analysis.

[0159] Control system: The core of the entire system, used to coordinate the operation of the five modules, material transmission, data processing and feedback adjustment to ensure the automation, efficiency and stability of the production process. The system integrates a variety of sensors to monitor the process parameters of each link in real time, and dynamically adjusts according to the feedback data to ensure the accuracy and consistency of sample processing.

[0160] like Figure 2 As shown, the first specific embodiment of the method for preparing a chromosome sample slide using the optimal parameter simulation method of the present invention is as follows:

[0161] A method for preparing chromosome sample slides, which automatically evaluates the slide making effect, automatically adjusts the process parameters such as culture, centrifugation, drop slide, digestion and staining as needed, and re-slides until the ideal slide making effect is obtained and then enters the batch slide making stage to achieve the best slide making effect; the specific contents are as follows:

[0162] Preliminary slide preparation: The sample enters the slide preparation and staining machine, and preliminary slide samples are prepared through operations such as dropping, baking, digestion and staining.

[0163] Slide preparation evaluation: The sample slide is transferred to the automatic microscopic scanning system, which uses the automatic scanning function to evaluate the number of metaphases, chromosome length, dispersion degree and banding effect on the slide.

[0164] Effect judgment: If the production effect does not meet the standard (such as small number of mitotic phases, too short chromosomes, severe clustering and crossing, or unclear banding), the system automatically enters the parameter adjustment stage; if the effect is qualified, the process enters the batch production stage.

[0165] Parameter adjustment: The system automatically adjusts key parameters such as incubation time and temperature, centrifugation speed and time, drop height and angle, digestion time and staining conditions.

[0166] Re-production: After adjusting the parameters, the system will re-produce the film and re-evaluate the production effect. If the effect is still unsatisfactory, the system will continue to adjust the parameters and re-produce until the result meets the requirements.

[0167] Batch production: Once the optimal production parameters are confirmed, the system will use these parameters as the standard for batch production to ensure that the quality of each batch of samples is consistent and suitable for subsequent karyotype analysis and clinical diagnosis.

[0168] like Figure 3 As shown, the second specific embodiment of the device for preparing chromosome sample slides of the present invention:

[0169] A chromosome sample slide preparation device consists of five main modules: an inoculation culture instrument, an automatic cell harvester, a slide preparation and staining all-in-one machine, an automatic cell microscopic scanning system and a control system.

[0170] Among them, the inoculation culture instrument, automatic cell harvester, slide preparation and staining machine, and automatic cell microscopy scanning system are all controlled by a unified control system, which automatically completes the process and transmission of materials from front to back under program control. The process and transmission process between each module are automated to ensure that the sample completes the preparation process at each stage in each submodule and the series process between each submodule is connected. Specifically, the processing mechanism of each module is as follows:

[0171] The inoculation incubator is used to automatically complete the inoculation and cultivation of samples. The specific processing flow includes:

[0172] Sample inoculation: The manually input blood sample is inoculated on the culture medium through the built-in robotic arm to ensure sterile operation.

[0173] Environmental control: The incubator has built-in temperature and humidity sensors that can automatically adjust the environmental conditions inside the box to maintain appropriate culture temperature and humidity.

[0174] Automatic transfer: After the culture is completed, the culture medium is automatically transferred by the conveyor belt to the next submodule, the automatic cell harvester.

[0175] The automatic cell harvester is responsible for receiving the culture medium from the inoculation culture instrument and performing a series of processing operations. The specific processing flow includes:

[0176] Add colchicine: The system automatically adds an appropriate amount of colchicine to block cell division.

[0177] Centrifugation: Samples are centrifuged to separate the cell pellet and supernatant.

[0178] Discard supernatant: Automatically aspirate to discard supernatant and retain cell pellet.

[0179] Add hypotonic oscillation: Automatically add hypotonic solution and swell the cells by oscillation.

[0180] Add fixative: automatically add fixative to fix cell morphology.

[0181] Centrifuge again and discard the supernatant: Perform a second centrifugation and discard the supernatant to ensure a purer cell population, providing better material for subsequent chromosome preparation and analysis.

[0182] Automatic transfer: After the automatic cell harvesting is completed, the system automatically transfers the samples to the slide preparation and staining machine.

[0183] The slide preparation and staining machine is used to prepare the processed cell samples on the slides and perform digestion and staining to obtain the final slide samples that can be used for chromosome scanning and analysis under the microscope system. The specific processing flow includes:

[0184] Slide preparation: The processed cell suspension is automatically dropped onto a glass slide by a pipette device, and the cells are spread and dispersed on the slide to form a thin layer for observation under a microscope.

[0185] Slide baking: The all-in-one machine integrates a slide baking device with adjustable temperature and time. It automatically places the prepared slides in the preheated slide baking device, dries the samples by constant temperature baking, and quickly fixes the cells, ensuring that the cell morphology and structure remain stable during the preparation process.

[0186] Digestion: Use a robot to grab the frosted area of ​​the slide, place the cell sample area of ​​the slide in a digestion tank, and use trypsin to treat the cells to break down the proteins between cells, separate the cells, remove the connecting substances between cells, and disperse the chromosomes for easy observation and analysis.

[0187] Rinsing: Wash the digested slides with an appropriate buffer (such as phosphate buffered saline) to remove residual enzymes, culture medium components or other impurities, which helps prevent background interference during staining and ensures the clarity of chromosomes.

[0188] Staining: The frosted area of ​​the slide is grabbed by a robot, and the cell sample area of ​​the slide is immersed in a staining jar. The chromosomes are stained with specific dyes (such as Giemsa dye) to make the banding characteristics of the chromosomes more visible and the structure clearer, which is convenient for chromosome analysis and identification.

[0189] Automatic transfer: Automatically transfer the prepared slides to the automatic cell microscopy scanning system.

[0190] The automatic cell microscopy scanning system is used to fully automatically scan and capture images of stained slides for chromosome karyotype analysis. The specific processing flow includes:

[0191] Automatic positioning: The fully automatic slide retrieval assembly pulls the slide to the stage, and the high-precision stage automatically positions the slide scanning area.

[0192] Intelligent scanning: The automatic microscopic imaging system includes a fully automatic stage, an automatic focusing system, optical imaging and a high-definition camera device, which automatically scans the slide scanning area in all directions to obtain high-definition cell images.

[0193] Data storage: Automatically store scanned cell images to facilitate chromosome karyotype analysis and archiving.

[0194] The control system is the core of the entire automated experimental device, which is used to coordinate the operation of each module to ensure the automated operation of the entire process and the unified management of data. It is mainly composed of a central processing unit (CPU), a data processing module, a sensor network, and a security monitoring module, and is responsible for the following functions:

[0195] System integration and process scheduling: The control system coordinates the work of various modules such as the inoculation culture instrument, cell harvester, slide preparation and staining machine, automatic cell microscopy scanning system, etc. in real time through the central processing unit (CPU) and embedded control program to ensure seamless connection according to the predetermined process flow.

[0196] Real-time monitoring and feedback adjustment: The control system integrates a variety of sensors (such as temperature, humidity, pressure, liquid level, position sensors, etc.) to monitor the status and process parameters of each module in real time. Through the feedback data of these sensors, the control system can dynamically adjust the operation of each sub-module. For example, when the temperature deviation during the culture process is collected, the system will automatically adjust the temperature in the incubator; during the preparation process, if the drop height or cell distribution is abnormal, the system will correct it by adjusting the movement of the robotic arm. This type of feedback adjustment mechanism ensures the accuracy and consistency of sample processing.

[0197] Data management and information traceability: The control system integrates a data management module to automatically record and track every step of the sample operation to ensure the uniqueness and traceability of the information. When the sample enters the system, the system will automatically identify and encode it to ensure the uniqueness of the sample information. The system tracks the processing status of the sample in each module, including culture time, harvesting conditions, preparation and staining parameters, to ensure the traceability of the entire process.

[0198] Operational safety and fault handling: The control system uses a built-in safety monitoring mechanism to detect the operating status of the equipment in real time to ensure safety during the work process. The sensor monitors key parameters such as temperature, pressure and liquid level in real time, and automatically alarms and shuts down when an abnormality occurs to prevent sample damage or equipment failure. The system has automatic shutdown and fault self-diagnosis functions, which can save current data, generate fault reports, and prompt operators to perform maintenance in the event of a fault, ensuring the continuity and safety of the work process.

[0199] The control system is not just a simple module coordination device. It ensures the efficient, stable and safe operation of the entire automated experimental device through efficient information processing, real-time monitoring feedback, refined data management and intelligent optimization functions. The system can monitor the progress of sample processing throughout the process and provide an immediate response mechanism in abnormal situations, thereby ensuring the accuracy and traceability of each sample from inoculation to final analysis. Through this highly integrated and intelligent control system, human intervention and operational errors are greatly reduced, and the laboratory's automation level, data reliability and sample processing efficiency are significantly improved.

[0200] A specific embodiment of the method and preparation device of the present invention is as follows:

[0201] The main factors that affect the preparation of chromosome slides include sample quality, inoculation and harvesting time, culture medium and various reagents used (such as colchicine, trypsin, etc.), preparation and staining operations, environmental control, etc. These factors will directly affect the quality of the final chromosomes, and thus affect the accuracy of subsequent analysis. Although some factors can be reasonably controlled through some equipment and standardized operations, changes in the performance of culture media and reagents may still affect the preparation effect, and the impact on the results cannot be completely eliminated.

[0202] To this end, the present invention adds a film scanning system with a film production effect evaluation function to the preparation device to ensure that the best film production effect is obtained in the batch film production process. When the system replaces different reagents, it first produces films according to the set film production conditions, and evaluates the film production effect by scanning the film. If the effect is not up to standard, the system will automatically adjust the film production parameters according to the set influencing factors, and re-produce until the film production effect meets the requirements. Finally, the system will achieve batch production based on the qualified film production parameters.

[0203] Slide preparation effect evaluation function: After slide preparation, the control system automatically transfers the sample slide to the slide scanning system for scanning and analysis. The system automatically detects the number and quality of metaphases on the slide, including chromosome length, dispersion, and banding staining. Slides with a large number of mitotic phases and good quality are more conducive to subsequent analysis and research, so the system will give priority to retaining such slides for karyotype analysis and clinical research. For samples with poor slide preparation, the system will automatically adjust based on influencing factors.

[0204] The main factors that affect the effect of chromosome slide preparation are as follows:

[0205] The effect of chromosome slide preparation is affected by many factors, including the quality of the sample itself, the control of environmental conditions during the preparation process, and the setting of various process parameters. Specifically, the following are several major influencing factors:

[0206] Sample quality: The health, activity and cell status of the sample directly affect the final film production results. Poor quality samples will lead to insufficient number of mitotic phases, affecting the distribution of chromosomes and banding effects.

[0207] Inoculation and harvesting time: The length of culture time and the timing of cell harvesting are crucial to the production effect. If the culture time after inoculation is too short, the cells may not be able to fully proliferate, resulting in insufficient number of mitotic phases; if the culture time is too long, the cells may enter the late mitotic phase, and the chromosomes may be too short to affect subsequent analysis.

[0208] Performance of culture medium and reagents: Colchicine, trypsin and other reagents play a role in blocking cell division and digesting intercellular matrix during the preparation process. The activity of reagents in different batches varies, which will have a direct impact on the state of cell division phase, chromosome dispersion and banding effect.

[0209] Centrifugation conditions: The centrifugal speed and time affect the sedimentation and concentration of cells. If the centrifugation conditions are inappropriate, it may lead to uneven distribution or excessive aggregation of cells, affecting the droplet effect.

[0210] Dropping operation: The height, angle and diffusion of the liquid during the drop directly determine the distribution of cells on the slide. If the drop height is too high, it may cause cell rupture and uneven chromosome dispersion; while if it is too low, it may cause cells to aggregate too much and chromosomes to overlap.

[0211] Digestion time: Digestion time determines the degree of chromosome separation. Too short a digestion time will result in incomplete chromosome separation and severe clustering and crossing; too long a digestion time may lead to over-digestion of chromosome structure and unsatisfactory banding and staining.

[0212] Staining time and environmental conditions: Staining time, dye concentration, temperature and humidity during the staining process affect the chromosome banding effect. If the staining time is too short, the banding will be unclear, while if the time is too long, the staining may be too dark, affecting the resolution of the chromosome.

[0213] Furthermore, the method and preparation device of the present invention are used to dynamically monitor the preparation effect, automatically optimize various key parameters, and ensure high-quality chromosome preparation. The specific process is as follows:

[0214] Step 1. Conduct film production evaluation. The specific method is as follows:

[0215] After completing the preliminary preparation, the system evaluates the preparation effect based on the preset preparation conditions (such as culture temperature, culture time, centrifugal speed and time, drop height and angle, digestion time, staining time, etc.). Through the scanning system, the number of metaphases, chromosome length, dispersion degree and banding quality are analyzed. If the evaluation results show that the number of mitotic phases is large, the chromosome length is moderate and the dispersion effect is good, it means that the preparation is qualified; if the evaluation does not meet the standards, the system will automatically adjust the corresponding parameters.

[0216] Step 2. Adjust the parameters. The specific method is as follows:

[0217] If the production effect is not ideal (such as small number of mitotic phases, chromosomes are too short, severe clustering and crossing, or unclear banding), the system will automatically adjust the following key parameters:

[0218] Culture time and temperature: If the number of mitotic phases is insufficient, the culture time of the cells can be appropriately extended, or the culture temperature can be fine-tuned to promote cell proliferation. Extending the culture time can increase the number of mitotic phases, and adjusting the temperature can optimize cell growth conditions.

[0219] Centrifugation speed and time: Centrifugation conditions affect the sedimentation state of cells. If the cells are unevenly distributed or over-aggregated, the system will reduce the centrifugation speed or shorten the centrifugation time to ensure that the cells are evenly distributed on the slide.

[0220] Droplet height and angle: If the chromosome dispersion is not ideal, the system will optimize the distribution of cells on the slide by adjusting the droplet height or angle. Adjusting the droplet height can improve cell breakage, while adjusting the angle helps cells spread evenly.

[0221] Digestion time: If the chromosomes are poorly dispersed or severely aggregated, the system will extend the digestion time to ensure effective chromosome separation; if the chromosomes are excessively banded, the digestion time should be appropriately shortened.

[0222] Staining time and conditions: If the band is not clear, the system will extend the staining time; if the staining is too dark, it will shorten the staining time. At the same time, the system will also check and adjust the temperature and humidity in the staining environment to ensure the best reaction effect of the stain.

[0223] Step 3. Re-filming and evaluation. The specific method is as follows:

[0224] After the parameters are adjusted, the system will re-perform the production operation and evaluate the production effect again. If the new production effect meets the standard (such as an increase in the number of mitotic phases, moderate chromosome length, good dispersion and clear banding), the system will confirm that the adjusted parameters are effective and enter the next stage; if the effect is still not ideal, the system will continue to make fine adjustments based on feedback until the expected quality is achieved.

[0225] Step 4. Batch production, the specific method is as follows:

[0226] Once the system confirms the optimal production parameters, the adjusted parameters will be used as the standard for subsequent batch production. The system will continue to monitor the quality of the batch production process to ensure that the production effect of each batch is consistent and stable. If the effect fluctuations are detected during the batch production process, the system will automatically make fine adjustments to maintain the high quality output of the samples.

[0227] The present invention can dynamically optimize various key parameters in the production process by combining production effect evaluation and automatic adjustment mechanism to ensure the best chromosome production effect under different conditions. Through real-time feedback and parameter adjustment, the system solves the influence of factors such as reagent variation and environmental fluctuation on the production effect, and significantly improves the stability, efficiency and repeatability of the experiment. After preliminary optimization, the system can achieve efficient batch production, ensure that the sample quality always meets the standard, and provide reliable sample support for subsequent karyotype analysis and clinical diagnosis.

[0228] The present invention provides an automated chromosome preparation device, which realizes the automation and high efficiency of the whole process by integrating the processes of culture, harvesting, slide preparation, staining, microscopic scanning, etc. Compared with the prior art, the present invention is simpler in operation and greatly improves the quality and consistency of slide preparation.

[0229] Furthermore, the present invention adopts an innovative overall integrated design, integrating multiple functional modules such as inoculation, cell harvesting, slide preparation, staining, and microscopic scanning into the same system. Each functional module is seamlessly connected through an automated transmission system, avoiding manual transfer operations between multiple devices. This highly integrated automated design greatly reduces manual intervention, reduces the risk of contamination and operational errors when samples are transferred between different devices, and significantly improves the convenience of operation and the consistency and stability of sample processing.

[0230] The present invention introduces an intelligent slide quality assessment and automatic adjustment mechanism, which can evaluate key indicators on the slide in real time (such as the number of metaphase divisions, chromosome dispersion and band clarity) through an automatic scanning system. If the slide production effect does not meet the standard, the system can automatically adjust the incubation time, centrifugation conditions, drop height, digestion and staining time and other process parameters, and re-slide until the best slide production effect is obtained. This innovative automatic adjustment mechanism ensures the quality consistency of each batch of slide production, greatly reduces the error of human adjustment, and improves the reliability and stability of slide production.

[0231] The present invention realizes the automation of the whole process from sample inoculation, cell harvesting to slide making and staining through an efficient automated operating system, reducing the complexity of manual operation. After optimizing the parameters, the system can perform batch slide making operations to ensure the consistency and efficiency of large-scale sample processing. This innovative automated design greatly improves the laboratory's sample processing capabilities, allowing the laboratory to process a large number of samples in a short period of time, significantly improving work efficiency and meeting the needs of high-throughput experiments.

[0232] Through the above-mentioned innovations, the present invention effectively solves the deficiencies in the prior art, realizes the automation and intelligence of the entire chromosome preparation process, ensures the high quality, stability and consistency of sample processing, and significantly improves the laboratory's work efficiency and operational reliability.

[0233] An embodiment of a device applying the method of the present invention:

[0234] An electronic device comprising:

[0235] one or more processors;

[0236] A storage device for storing one or more programs;

[0237] When the one or more programs are executed by the one or more processors, the one or more processors implement the above-mentioned optimal parameter simulation method for preparing a chromosome sample slide.

[0238] A computer medium embodiment using the method of the present invention:

[0239] A computer-readable storage medium stores a computer program, which, when executed by a processor, implements the above-mentioned optimal parameter simulation method for preparing a chromosome sample slide.

[0240] Those skilled in the art will appreciate that the embodiments of the present application may be provided as methods, systems, and computer program products. Therefore, the present application may take the form of a complete hardware embodiment, a complete software embodiment, or an embodiment combining software and hardware. Moreover, the present application may take the form of a computer program product implemented on one or more computer-usable storage media (including but not limited to disk storage, optical storage, etc.) containing computer-usable program codes.

[0241] The present application is described by flowcharts or / and block diagrams of the methods, devices (systems), and computer program products of the embodiments of the present application. It should be understood that each process or / and block in the flowchart or / and block diagram and the combination of the processes or / and blocks in the flowchart or / and block diagram can be implemented by computer program instructions. These computer program instructions can be provided to a processor of a general-purpose computer, a special-purpose computer, an embedded processor, or other programmable data processing device to generate a machine, so that the instructions executed by the processor of the computer or other programmable data processing device generate instructions for implementing the processes in the flowchart or / and block diagram. Figure 1 Process or multiple processes and / or boxes Figure 1 A device that provides the functions specified in a block or multiple blocks.

[0242] These computer program instructions may also be stored in a computer-readable memory capable of directing a computer or other programmable data processing device to operate in a specific manner, so that the instructions stored in the computer-readable memory produce an article of manufacture comprising an instruction device, which implements the process Figure 1 Process or multiple processes and / or boxes Figure 1A function specified in one or more boxes.

[0243] These computer program instructions can also be loaded onto a computer or other programmable data processing device so that a series of operating steps are executed on the computer or other programmable device to produce a computer-implemented process, thereby providing instructions for implementing the process. Figure 1 Process or multiple processes and / or boxes Figure 1 The steps for the functions specified in one or more boxes.

[0244] The model in this application is an object that objectively describes the morphological structure with the help of physical or virtual representation. The object is not equal to the object and is not limited to physical and virtual. It can be a data processing function, software program, processing mode, usage method, operation method, workflow, application process, electronic hardware, circuit module, processing system, system imitation or simulation object.

[0245] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field can still modify or replace the specific implementation methods of the present invention with equivalents. Any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A method for simulating optimal parameters for preparing chromosome sample slides, characterized in that: The following steps are involved: Step 1, obtaining the slide preparation parameters of a chromosome sample during the slide preparation process; Step 2: Scan and evaluate the chromosome sample slide obtained based on the slide production parameters using a pre-built slide production evaluation model to obtain a slide evaluation result; Step 3: using the pre-built effect judgment model, judging whether the slide production effect meets the standard according to the slide evaluation result, if the slide production effect meets the standard, outputting the current slide production parameters and executing step 6; If the production effect does not meet the standard, proceed to step 4; Step 4: using the pre-built parameter adjustment model, based on the slide making effect, adjusting the slide making parameters in the chromosome sample slide preparation process, determining new slide making parameters, and executing step 2; Step five, using the current slide making parameters as the optimal parameters for batch slide making, and using them for batch slide making to achieve the optimal parameter simulation for the preparation of chromosome sample slides.

2. The method for simulating optimal parameters for preparing a chromosome sample slide according to claim 1, characterized in that: Step 2: Scan and evaluate the chromosome sample slide obtained based on the slide production parameters using a pre-built slide production evaluation model to obtain a slide evaluation result; Obtaining the slide preparation parameters, including incubation temperature, incubation time, centrifugation speed and time, drop height and angle, digestion time and staining time; Based on the production parameters, the chromosome preparation device is set up; Obtaining a chromosome sample slide through the set-up chromosome preparation device; The chromosome sample slide is transferred to the scanning system for scanning and analysis, and the number and quality of the chromosome metaphase division phases on the chromosome sample slide are detected. The number and quality of the chromosome metaphase division phases at least include the number of metaphase division phases, chromosome length, dispersion and banding staining conditions; The chromosome length, dispersion and banding staining are coupled to form the slide evaluation results.

3. The method for simulating optimal parameters for preparing a chromosome sample slide according to claim 1, characterized in that: Step 3: Using the pre-built effect judgment model, the method for judging whether the slide production effect meets the standard according to the slide evaluation results is as follows: Obtaining film production evaluation standards, which at least include the standard number of metaphase divisions, standard chromosome length, standard dispersion, and standard banding quality; Compare the slide evaluation criteria with the slide evaluation results to analyze whether the number of metaphases, chromosome length, dispersion, and banding quality in the chromosome sample slides meet the standards; If the number of metaphases, chromosome length and dispersion effect in the chromosome sample slide exceed the slide preparation evaluation standard, it means that the slide preparation effect meets the standard, and the slide preparation parameters at this time are output; If the number of metaphases, chromosome length and dispersion effect in the chromosome sample slide do not meet the production evaluation standards, it means that the production effect is not up to standard, that is, the number of metaphases is small, the chromosomes are too short, the clustering and crossing are serious, or the banding is not clear.

4. The method for simulating optimal parameters for preparing a chromosome sample slide according to claim 1, characterized in that: Step 4: Using the pre-built parameter adjustment model, the method for adjusting the slide preparation parameters in the chromosome sample slide preparation process based on the slide preparation effect is as follows: Adjust the incubation time and temperature, including the following: If the number of mitotic phases is insufficient, extend the cell culture time or fine-tune the culture temperature to promote cell proliferation; extend the culture time to increase the number of mitotic phases, and adjust the temperature to optimize cell growth conditions; Adjust the centrifugation speed and time, including the following: Centrifugation conditions will affect the sedimentation state of cells; if cells are unevenly distributed or aggregated, reduce the centrifugation speed or shorten the centrifugation time to ensure that the cells are evenly distributed on the chromosome sample slide; Adjust the drop sheet height and angle, which includes the following: If the chromosome dispersion is not ideal, optimize the distribution of cells on the chromosome sample slide by adjusting the drop height or angle; adjusting the drop height improves cell rupture, while adjusting the angle helps cells spread evenly; Adjust digestion time, which includes the following: For cases of poor chromosome dispersion and aggregation, the digestion time should be extended to ensure effective chromosome separation; if the chromosomes are excessively banded, the digestion time should be shortened; Adjust the staining time and conditions, including the following: If the band is not clear, extend the staining time; if the staining is too dark, shorten the staining time; at the same time, check and adjust the temperature and humidity in the staining environment to ensure the best reaction effect of the stain.

5. A method for simulating optimal parameters for preparing chromosome sample slides, characterized in that: Includes the following: Through the pre-built slide evaluation model, the chromosome sample slide is scanned and evaluated to obtain the slide evaluation result; Use the pre-built effect judgment model to judge whether the slide production effect meets the standard based on the slide evaluation results; If the slide preparation effect does not meet the standard, the pre-built parameter adjustment model is used to adjust the equipment parameters in the chromosome sample slide preparation process based on the slide preparation effect to obtain new slide preparation parameters; Use the pre-built re-slide model to obtain new chromosome sample slides based on new slide making parameters, and re-scan and evaluate the slide making results; If the production effect is up to standard, the production parameters at this time are output as the optimal parameters for batch production, which are used for batch production to ensure the consistency of the quality of chromosome samples in each batch and realize the simulation of the optimal parameters for the preparation of chromosome sample slides.

6. A device for preparing a chromosome sample slide, characterized in that: Including inoculation culture instrument, automatic cell harvester, slide preparation and staining machine, automatic cell microscopy scanning system and control system; Inoculation and culture instrument, used to complete the inoculation and culture of chromosome samples; An automatic cell harvester, used to receive culture medium from an inoculated culture instrument and perform a series of processing operations; The slide making and staining machine is used to make the processed chromosome samples on the glass slides and digest and stain them to obtain the sample slides that can be used for chromosome scanning and analysis; An automated cell microscopy scanning system for scanning and image capture of stained slides for chromosome karyotype analysis; The control system includes a central processing unit, a sensor network, a data processing module, and a safety monitoring module, which is used to coordinate the operation of the inoculation culture instrument, the automatic cell harvester, the slide preparation and staining machine, and the automatic cell microscopy scanning system to ensure the operation of the entire process and the unified management of data.

7. The device for preparing a chromosome sample slide according to claim 6, characterized in that: Inoculation culture apparatus, including a robotic arm, an incubator, and a conveyor belt; The incubator is equipped with temperature and humidity sensors and culture medium; The blood sample is inoculated on the culture medium through the built-in robotic arm to complete the inoculation of chromosome samples and ensure aseptic operation; The temperature and humidity sensors can collect the temperature and humidity in the incubator to adjust the environmental conditions in the incubator and maintain appropriate culture temperature and humidity; After the culture is completed, the culture medium is transferred to the automatic cell harvester by a conveyor belt; Or / and, an automatic cell harvester for receiving culture medium from an inoculation culture instrument and performing a series of processing operations as follows: For chromosome samples in the culture medium, add an appropriate amount of colchicine to block cell division; The chromosome samples were centrifuged to separate the cell pellet and the supernatant; Aspirate the chromosome sample to discard the supernatant and retain the cell pellet; Add hypotonic solution to the chromosome sample and swell the cells by shaking; Add fixative to the chromosome sample to fix the cell morphology; Perform a second centrifugation on the chromosome sample and discard the supernatant to ensure a pure cell suspension, providing better materials for subsequent chromosome preparation and analysis; After the chromosome sample is harvested, the chromosome sample is transferred to the slide preparation and staining machine.

8. The device for preparing a chromosome sample slide according to claim 7, characterized in that: The slide making and staining machine includes a liquid transfer device, a slide baking device, a manipulator, a digestion tank and a staining tank; Use a pipette to drop the treated cell suspension onto a glass slide, and spread the cells on the glass slide to form a thin layer for easy observation under a microscope; The baking device is used to adjust the temperature. The prepared slides are placed in the preheated baking device. The chromosome samples are dried by baking at a constant temperature to quickly fix the cells and ensure that the morphology and structure of the cells remain stable during the preparation process. The frosted area of ​​the slide is grabbed by a robot, and the chromosome sample is placed in a digestion tank. The cells are treated with trypsin to decompose the proteins between cells, separate the cells, and remove the connecting substances between cells, so that the chromosomes can be dispersed for easy observation and analysis; Wash the digested slides with buffer to remove residual enzymes, culture medium components or impurities, prevent background interference during staining, and ensure the clarity of chromosomes; The frosted area of ​​the slide is grabbed by a robot, and the chromosome sample is immersed in a staining jar. The chromosome is stained with a specific dye to make the chromosome banding characteristics visible and the chromosome structure clear, which is convenient for chromosome analysis and identification. Transfer the prepared slides to the automated cell microscopy scanning system; or / and, an automatic cell microscopic scanning system, comprising a film retrieval component, a stage, an automatic microscopic imaging system and a storage component; The slide retrieval assembly pulls the slide to the stage, and the stage locates the slide scanning area; The automatic microscopic imaging system includes a fully automatic stage, an automatic focusing system, optical imaging and a high-definition camera device, which can perform a full-scale scan of the slide scanning area to obtain high-definition cell images; The cell images scanned by the storage component are convenient for chromosome karyotype analysis and archiving.

9. The device for preparing a chromosome sample slide according to claim 8, characterized in that: The control system coordinates the work of the inoculation culture instrument, cell harvester, slide preparation and staining machine, and automatic cell microscopy scanning system in real time through the central processing unit and embedded control program to ensure seamless connection according to the predetermined process flow; The control system integrates a sensor network, which includes at least a temperature sensor, a humidity sensor, a pressure sensor, a liquid level sensor and a position sensor, for real-time monitoring of the status and process parameters of each module; Through the feedback data from these sensors, the control system can dynamically adjust the operation of each submodule; That Includes the following: When a temperature deviation is detected during the culture process, the temperature in the incubator is adjusted; During the preparation process, if the drop height or cell distribution is abnormal, it can be corrected by adjusting the movement of the robotic arm; this feedback adjustment mechanism ensures the accuracy and consistency of chromosome sample processing; The control system integrates a data processing module to automatically record and track every step of the chromosome sample operation to ensure the uniqueness and traceability of the information. First, the chromosome sample is identified and encoded to ensure the uniqueness of the chromosome sample information. Then the processing status of the chromosome sample in each module is tracked, including culture time, harvesting conditions, preparation and staining parameters, to ensure the traceability of the entire process. The control system uses a built-in safety monitoring module to detect the operating status of the equipment in real time to ensure safety during the working process; the sensor network monitors key parameters in real time, including temperature, pressure and liquid level. When an abnormality occurs, it will alarm and shut down to prevent damage to chromosome samples or expansion of equipment failures; the control system has automatic shutdown and fault self-diagnosis functions, which can save current data when a fault occurs, generate a fault report, and prompt operators to perform maintenance to ensure the continuity and safety of the work process.

10. A computer-readable storage medium, characterized in that: A computer program is stored thereon, and when the program is executed by a processor, a method for simulating optimal parameters for preparing a chromosome sample slide as described in any one of claims 1 to 5 is implemented.

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