Fish stimulation response behavior tracking and monitoring device and method

Abstract

The invention discloses a fish stimulus response behavior tracking and monitoring device and method.The device comprises an observation box and an animal behavior trajectory tracking system in communication connection with the observation box, the observation box comprises a box body with one hollow side, a supporting frame is arranged in the box body, a bearing plate is attached to the top of the supporting frame, and the bearing plate is connected with the observation box in a sliding mode; a perforated plate is inserted into the bearing plate, a stimulation mechanism for stimulating fishes in the perforated plate is arranged in the supporting frame, an infrared camera is arranged on the inner wall of the box body above the supporting frame, and the bearing plate is movably arranged in the box body through a driving mechanism; an auxiliary mechanism is arranged on the inner wall of the top of the box body and located on the outer side of the infrared camera. The auxiliary mechanism is arranged, after tracking and monitoring work is finished, the auxiliary mechanism can replace manual work to transfer juvenile zebrafish located in the perforated plate into the containing container, time is saved, and meanwhile the manual labor intensity is greatly reduced.

Description

Technical Field

[0001] The invention relates to the technical field of fish tracking and monitoring, and in particular to a device and method for tracking and monitoring fish stimulus response behavior. Background Art

[0002] The application of fish stimulus response behavior tracking monitoring device in the field of CYN research has been very extensive. Cylindrospermopsin (CYN), as a common cyanobacterial toxin, has attracted much attention for its effects on aquatic organisms, especially fish. In current research, users usually expose zebrafish fry to different concentrations of CYN exposure solution continuously, and after a certain period of time, these fry are placed in the fish stimulus response behavior tracking monitoring device for observation and analysis.

[0003] The fish tracking and monitoring device mainly consists of a zebrafish observation box and a computer pre-installed with an animal behavior trajectory tracking system (including an experimental hardware control module and a multi-observation zone module). The data of the fish's reaction tests such as light stimulation and sound stimulation in the observation box are collected to the animal behavior trajectory tracking system. The animal behavior trajectory tracking system collects and analyzes the movement trajectory of the young fish, the activity heat map, and calculates key parameters such as the movement distance, stagnation time and movement speed, so as to help users understand the impact of CYN on the early development of the zebrafish nervous system. However, although the above-mentioned tracking and monitoring device can track and monitor fish, it still has the following defects when used:

[0004] The zebrafish fry to be tracked and monitored are usually placed in the holes of a porous plate and then placed in an observation box. After the work is completed, the zebrafish fry in the porous plate need to be transferred. At present, the zebrafish in the porous plate holes are usually manually extracted one by one by a straw and then transferred to an external container. This method is not only time-consuming, but also labor-intensive. After the zebrafish in the porous plate is transferred, in order to ensure the next use of the porous plate, it needs to be manually cleaned and disinfected, which further increases the workload of the user. To this end, we propose a device and method for tracking and monitoring fish stimulus response behavior. Summary of the invention

[0005] The purpose of the present invention is to provide a device and method for tracking and monitoring fish stimulus response behavior to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A fish stimulation response behavior tracking and monitoring device comprises an observation box and an animal behavior trajectory tracking system communicatively connected to the observation box, wherein the animal behavior trajectory tracking system is pre-configured in a virtual environment and is used to receive and analyze and process fish behavior trajectory data collected by the observation box, the observation box comprises a box body with a hollow side, a support frame is provided in the box body, a carrying plate is attached to the top of the support frame, a porous plate for placing fish is inserted on the carrying plate, the lower end of the porous plate extends into the support frame, a stimulation mechanism for stimulating fish in the porous plate is provided in the support frame, an infrared camera is provided on the inner wall of the box body above the support frame, the carrying plate is movably arranged in the box body by a driving mechanism, an auxiliary mechanism is provided on the inner wall of the top of the box body and located on the outside of the infrared camera, the auxiliary mechanism is used to collect fish located in the porous plate when the driving mechanism drives the carrying plate to move to a preset position.

[0008] A further improvement is that the stimulation mechanism includes a light stimulation unit, a sound stimulation unit and a vibration stimulation unit; it also includes a water supply pipe with one end connected to the inner cavity of the support frame and the other end connected to an external water supply device and a heater arranged in the support frame, and the support frame is also connected to an external pump body through a drainage pipeline.

[0009] A further improvement is that the driving mechanism comprises:

[0010] Two groups of movable bases are respectively located at the two ends of the bottom of the bearing plate and are slidably connected to the inner wall of the bottom of the box body. The two groups of movable bases are respectively threadedly sleeved on the outer walls of the two groups of screws. The screws are longitudinally rotatably arranged in the box body, and one end of the screws movably penetrates the box body and is connected to the output end of the rotating device; and,

[0011] The two sets of telescopic devices are respectively arranged at two ends of the top of the bearing plate, and the output ends of the two sets of telescopic devices penetrate the bearing plate and are respectively connected with the two sets of movable bases.

[0012] A further improvement is that the auxiliary mechanism comprises:

[0013] A top plate is detachably mounted on the top inner wall of the box body, the bottom of the top plate is connected to the bottom plate via a connecting rod, a plurality of adsorption cylinders are detachably mounted on the bottom plate, the lower end of the adsorption cylinder is connected to an adsorption head for entering the holes of the porous plate, a piston is mounted in the adsorption cylinder, and the piston passes through the top of the adsorption cylinder via a piston rod; and,

[0014] The second telescopic device is arranged at the bottom of the top plate. A movable plate is arranged at the output end of the second telescopic device. The movable plate is detachably connected to the piston rod.

[0015] A further improvement is that the vertical cross-section of the porous plate is T-shaped, and a T-shaped opening for placing the porous plate is provided on the supporting plate. The length of the supporting plate is greater than the length of the supporting frame and less than the length of the inner cavity of the box cover. Box bodies are symmetrically and slidably provided on both sides of the top of the supporting plate. The opposite sides and the bottom of the two groups of box bodies are hollow. The opposite sides of the two groups of box bodies are both fitted with three output ends of a telescopic device. The three telescopic devices are fixedly arranged on the inner wall of the box body for driving the two groups of box bodies to contact each other. The opposite sides of the two groups of box bodies are also connected to the top of the supporting plate through an elastic member for driving the box bodies to reset. The top inner walls of the two groups of box bodies are provided with diverter plates, and several groups of nozzles are provided at the bottom of the diverter plates. One of the diverter plates is provided with a connected docking pipe on the side close to the porous plate, and the other diverter plate is provided with a docking hole for inserting the docking pipe on the side close to the porous plate. One of the diverter plates is connected to the external drying equipment and disinfectant supply equipment through a circulation pipeline.

[0016] A further improvement is that the inner walls of the two groups of the box bodies are vertically provided with electric guide rail mechanisms, and an elastic telescopic rod is horizontally rotatably provided on the slider of the electric guide rail mechanism, and a pressure plate for contacting the porous plate is provided at the other end of the elastic telescopic rod, and a gear is sleeved on the outer wall of the fixed section of the elastic telescopic rod, and a rack is provided on the guide rail of the electric guide rail mechanism, which is used to drive the gear to drive the elastic telescopic rod to rotate 180° when the slider drives the elastic telescopic rod upward to a preset position.

[0017] A further improvement is that a lens frame is provided on the inner wall of the box body through rotation of a rotator, and a lens body for covering the top of the porous plate is embedded on the lens frame.

[0018] A further improvement is that a box cover for closing the hollow side of the box body is provided on one side of the box body.

[0019] A method for tracking and monitoring fish stimulus response behavior, using the above device, comprises the following steps:

[0020] S1: When in use, a number of zebrafish fry that have been continuously exposed to CYN exposure solutions of different concentrations for a preset time are placed in a porous plate containing embryo culture water, the porous plate is placed on a carrier plate, and the carrier plate is driven to the support frame by a driving mechanism;

[0021] S2: stimulating the zebrafish fry in the multi-hole plate through the stimulation mechanism and collecting the movement trajectory data of the zebrafish fry through the infrared camera and sending the movement trajectory data to the animal behavior trajectory tracking system for analysis and processing;

[0022] Among them, after the tracking and monitoring work is completed, when the carrying plate is moved to the preset position by the driving mechanism, the zebrafish fry located in the porous plate is sucked by the auxiliary mechanism, and then the zebrafish fry is transferred to an external placement container by the auxiliary mechanism.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1) The present invention stimulates the zebrafish fry in the porous plate through a stimulation mechanism and collects the motion trajectory data of the zebrafish fry through an infrared camera and sends the data to the animal behavior trajectory tracking system for analysis and processing, thereby realizing the tracking and monitoring of the fish. At the same time, an auxiliary mechanism is provided. After the tracking and monitoring work is completed, the auxiliary mechanism can replace manual labor to transfer the zebrafish fry in the porous plate to a placement container, which not only saves time but also greatly reduces the labor intensity;

[0025] 2) The present invention is also provided with a supporting plate, a box body, a telescopic device three, an elastic member, a circulation pipeline and a diverter plate and other structures, so that the porous plate can be disinfected and cleaned by the disinfectant liquid supplied by the disinfectant supply device in the space formed by the combination of the two sets of box bodies and dried by the drying equipment, without manual processing, further reducing the labor intensity and improving the applicability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the structure of the observation box of the present invention;

[0027] Figure 2 For the present invention Figure 1 Schematic diagram of the internal structure;

[0028] Figure 3 For the present invention Figure 2 Schematic diagram of the local structure in;

[0029] Figure 4 It is a schematic diagram of the driving mechanism structure of the present invention;

[0030] Figure 5 It is a schematic diagram of the auxiliary mechanism structure of the present invention;

[0031] Figure 6 It is a schematic diagram of the structure of the electric guide rail mechanism of the present invention.

[0032] In the figure: 1. box body; 101. box cover; 2. support frame; 3. water supply pipe; 4. bearing plate; 5. porous plate; 6. screw; 7. movable base; 8. telescopic device one; 9. auxiliary mechanism; 91. top plate; 92. bottom plate; 93. adsorption cylinder; 94. adsorption head; 95. movable plate; 96. telescopic device two; 10. lens body; 11. box body; 12. telescopic device three; 13. elastic member; 14. infrared camera; 15. diverter plate; 16. circulation pipeline; 17. docking pipe; 18. electric guide rail mechanism; 19. elastic telescopic rod; 20. rack; 21. gear; 22. pressure plate. DETAILED DESCRIPTION

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0034] Please refer to the attached Figure 1 - attached Figure 2

[0035] A fish stimulus response behavior tracking and monitoring device includes an observation box and an animal behavior trajectory tracking system communicatively connected to the observation box. Among them, the animal behavior trajectory tracking system is pre-configured in a virtual environment (such as a computer) and is used to receive and analyze the fish behavior trajectory data collected by the observation box. The animal behavior trajectory tracking system includes a test hardware control module, a multi-observation area module, etc., which belong to the prior art and will not be elaborated here.

[0036] The observation box includes a box body 1 with a hollow side. One side of the box body 1 is provided with a box cover 101 for closing its hollow side. A support frame 2 is arranged inside the box body 1. The support frame 2 is in a shape of a rectangle with a hole in the middle. The top of the support frame 2 is attached to a bearing plate 4. A porous plate 5 for placing fish is inserted on the bearing plate 4. The lower end of the porous plate 5 extends into the support frame 2. A stimulating mechanism for stimulating the fish in the porous plate 5 is arranged inside the support frame 2. Preferably, the stimulating mechanism includes a light stimulation unit, a sound stimulation unit, and a vibration stimulation unit. Among them, the light stimulation unit is, for example, an adjustable light source, such as a TTL-controlled white light source. These light sources can be automatically turned on and off and the brightness can be adjusted according to requirements, and are used to study the physiological behaviors of zebrafish such as circadian rhythm and startle response. The sound stimulation unit includes a speaker arranged on the outer wall of the support frame 2, and the fish is stimulated by playing sound through the speaker. The vibration stimulation unit includes a vibrator with adjustable vibration strength, and the fish is vibrationally stimulated by applying different vibration strengths to the porous plate 5 through the vibrator. Of course, the above-mentioned light stimulation unit, sound stimulation unit, and vibration stimulation unit are not limited to these implementation methods.

[0037] The stimulating mechanism further includes a water supply pipe 3 with one end communicating with the inner cavity of the support frame 2 and the other end communicating with an external water supply device, and a heater arranged inside the support frame 2. The external water supply device can inject liquid into the support frame 2 through the water supply pipe 3. The injected liquid enters the support frame 2 and contacts the porous plate 5. The water temperature inside the support frame 2 can be controlled by the heater so as to better obtain the fish behavior trajectory data. The support frame 2 is also connected to an external pump body through a drainage pipeline to drain the liquid inside the support frame 2.

[0038] An infrared camera 14 is provided on the inner wall of the box body 1 above the supporting frame 2, and the carrying plate 4 is movably arranged in the box body 1 through a driving mechanism. An auxiliary mechanism 9 is provided on the inner wall of the top of the box body 1 and on the outside of the infrared camera 14. The auxiliary mechanism 9 is used to collect the fish located in the porous plate 5 when the driving mechanism drives the carrying plate 4 to move to a preset position. The auxiliary mechanism 9 can assist the user in collecting and transferring the fish in the porous plate 5 after the tracking and monitoring work is completed, thereby reducing the intensity of manual labor.

[0039] Please see attached Figure 3 -Attached Figure 4

[0040] Preferably, the driving mechanism of this embodiment includes:

[0041] Two groups of movable bases 7 are respectively located at the two ends of the bottom of the carrying plate 4 and are slidably connected to the inner wall of the bottom of the box body 1. The two groups of movable bases 7 are respectively threadedly sleeved on the outer walls of the two groups of screw rods 6. The screw rods 6 are longitudinally rotatably arranged in the box body 1, and one end of the screw rods 6 movably penetrates the box body 1 and is connected to the output end of the rotating device (not shown in the figure). The rotating device is, for example, a servo motor and a reducer. By turning on the rotating device to drive the screw rods 6, the movable base 7 can drive the carrying plate 4 to move; and,

[0042] Two sets of telescopic devices 8 are respectively arranged at two ends of the top of the carrying plate 4, and the output ends of the two sets of telescopic devices 8 both penetrate the carrying plate 4 and are respectively connected to the two sets of movable bases 7.

[0043] When in use, the supporting plate 4 is driven upward by the telescopic device 8, so that the porous plate 5 is upward and separated from the supporting frame 2, and then the movable base 7 is driven by the rotating device and the screw 6 to drive the supporting plate 4 to move to the auxiliary mechanism 9, and then the supporting plate 4 is driven upward by the telescopic device 8 to correspond to the auxiliary mechanism 9, and the fish in the porous plate 5 can be collected and transferred by the auxiliary mechanism 9.

[0044] Please see attached Figure 5

[0045] Preferably, the auxiliary mechanism 9 of this embodiment includes:

[0046] A top plate 91 is detachably arranged on the top inner wall of the box body 1. The top plate 91 can be connected to the top inner wall of the box body 1 by a snap-fit ​​structure. The bottom of the top plate 91 is connected to a bottom plate 92 by a connecting rod. A plurality of groups of adsorption cylinders 93 are detachably arranged on the bottom plate 92. The bottom plate 92 and the adsorption cylinders 93 can be fixed by bolts or snap-fit ​​structures. Specifically, a plurality of groups of adsorption cylinders 93 are arranged linearly, and the number thereof can correspond to the number of holes in a row on the porous plate 5. The lower end of the adsorption cylinder 93 is connected to an adsorption head 94 for entering the holes in the porous plate 5. A piston is arranged in the adsorption cylinder 93. The piston passes through the top of the adsorption cylinder 93 through a piston rod. The piston is driven by the piston rod to move in the adsorption cylinder 93, so that the liquid and fish in the porous plate 5 can be sucked into the adsorption cylinder 93. When the liquid and fish are transferred to other containers, the liquid and fish can be discharged by pressing the piston; and,

[0047] The telescopic device 2 96 is arranged at the bottom of the top plate 91. A movable plate 95 is arranged at the output end of the telescopic device 2 96. The movable plate 95 is detachably connected to the piston rod. The movable plate 95 and the piston rod can be connected by a snap-fit ​​structure. The movable plate 95 can be driven by the telescopic device 2 96 to drive the piston rod to move upward. It should be noted that the telescopic device 2 96 drives the movable plate 95 to move a short distance each time, that is, to extract the liquid and fish in the holes of the porous plate 5. After the liquid and fish in the holes of a row in the porous plate 5 are extracted, the rotating device and the screw 6 can continue to drive the bearing The carrier plate 4 moves so that another row of holes on the porous plate 5 corresponds to the adsorption cylinder 93. After all the fish in the porous plate 5 are extracted, the user can remove the auxiliary mechanism 9 from the box body 1 or place a placement container under the adsorption cylinder 93. By aligning the adsorption cylinder 93 with the placement container to be transferred, and then driving the movable plate 95 to drive the piston rod to move downward and reset, the liquid and fish in the adsorption cylinder 93 can be discharged into the placement container. The user can also remove the required adsorption cylinder 93 separately as needed and transfer the fish in the adsorption cylinder 93 to the required position.

[0048] Preferably, the porous plate 5 of the present embodiment is T-shaped in vertical section, and a T-shaped opening for placing the porous plate 5 is provided on the supporting plate 4, so that the porous plate 5 can be easily disassembled and installed in this way; the length of the supporting plate 4 is greater than the length of the supporting frame 2 and less than the length of the inner cavity of the box cover 101, and box bodies 11 are symmetrically slidably provided on both sides of the top of the supporting plate 4, and the opposite sides and the bottom of the two groups of box bodies 11 are hollow, and a sealing strip can be embedded in the outer wall position where the box body 11 contacts the supporting plate 4 to ensure the sealing between the box body 11 and the supporting plate 4, and the opposite sides of the two groups of box bodies 11 are both fitted with the output end of a telescopic device three 12, and the telescopic device three 12 is fixedly arranged on the inner wall of the box body 1, and is used to drive the two groups of box bodies 11 to contact, and when the two groups of box bodies 11 are in contact, the porous plate 5 is in the two groups of box bodies 11, and the opposite sides of the two groups of box bodies 11 are also connected to the top of the supporting plate 4 by an elastic member 13, which is used to drive the box body 11 to reset, and the elastic member 13 is, for example, a spring , the top inner walls of the two groups of box bodies 11 are both provided with a diverter plate 15, and the bottom of the diverter plate 15 is provided with a plurality of groups of nozzles, wherein a diverter plate 15 is provided with a connected docking pipe 17 on one side close to the porous plate 5, and another diverter plate 15 is provided with a docking hole for inserting the docking pipe 17 on one side close to the porous plate 5. When the two groups of box bodies 11 are in contact, the docking pipe 17 of one diverter plate 15 enters the docking hole of the other diverter plate 15, and then the two diverter plates 15 are connected to each other, wherein one diverter plate 15 is connected to an external drying device and a disinfectant supply device through a circulation pipeline 16, and the circulation pipeline 16 may include a pipeline and a three-way pipeline connector, and the two ends of the three-way pipeline connector are respectively connected to the drying device and the disinfectant supply device through pipelines, and a valve body is also provided on the corresponding pipeline; the drying device is, for example, a heater, and the disinfectant supply device includes, for example, a box body storing a disinfectant and a pump body for inputting the disinfectant in the box body into the circulation pipeline 16;

[0049] After the fish and liquid in the porous plate 5 are collected by the auxiliary mechanism 9, the porous plate 5 is driven to return to the support frame 2 by the rotating device and the screw 6, and the two groups of box bodies 11 are driven to contact each other by the telescopic device 12, and then the disinfectant liquid is supplied by the disinfectant supply device. The disinfectant liquid enters the diverter plate 15 and is then sprayed out from the nozzle to clean and disinfect the porous plate 5. After disinfection, an airflow with a certain amount of heat can be supplied by a drying device to dry the porous plate 5.

[0050] Please see attached Figure 6

[0051] Preferably, the inner walls of the two groups of box bodies 11 of the present embodiment are vertically provided with an electric guide rail mechanism 18, which belongs to the prior art and includes structures such as a guide rail, a slider, and an electric motor. An elastic telescopic rod 19 is horizontally rotatably provided on the slider of the electric guide rail mechanism 18, and a pressure plate 22 for contacting the porous plate 5 is provided at the other end of the elastic telescopic rod 19. The cross section of the pressure plate 22 can be T-shaped, and a groove for the pressure plate 22 to enter can be provided on the side wall of the porous plate 5. In the process of the two groups of box bodies 11 approaching each other, the elastic telescopic rod 19 is pressed so that the pressure plate 22 is tightly in contact with the porous plate 5. The outer wall of the fixed section of the elastic telescopic rod 19 is provided with a gear 21, and the electric guide rail mechanism 18 is provided with a plurality of gears. A rack 20 is provided on the guide rail of the rail mechanism 18, which is used to drive the gear 21 to drive the elastic telescopic rod 19 to rotate 180° when the slider drives the elastic telescopic rod 19 upward to a preset position. After the two groups of box bodies 11 contact each other, the slider is driven to move upward by the electric guide rail mechanism 18, and then the elastic telescopic rod 19 and the pressure plate 22 drive the porous plate 5 to move upward and away from the carrier plate 4. In the upward process, the gear 21 and the rack 20 cooperate, so that the elastic telescopic rod 19 and the pressure plate 22 drive the porous plate 5 to rotate 180°, which not only allows the disinfection liquid in the porous plate 5 to separate from the porous plate 5 and enter the support frame 2, but also allows the porous plate 5 to be fully disinfected and dried.

[0052] Preferably, the inner wall of the box body 1 of the present embodiment is provided with a lens frame which is rotated by a rotator, and the rotator is, for example, a rotary motor. A lens body 10 for covering the top of the porous plate 5 is embedded in the lens frame. The lens frame is driven to rotate to a horizontal state by the rotator, so that the lens body 10 can correspond to and fit on the top of the porous plate 5, and the infrared camera 14 can better track and monitor the fish through the lens body 10.

[0053] A method for tracking and monitoring fish stimulus response behavior, using the above device, comprises the following steps:

[0054] S1: When in use, a plurality of zebrafish fry that have been continuously exposed to CYN exposure solutions of different concentrations for a preset time are placed in a porous plate 5 containing embryo culture water, the porous plate 5 is placed on a carrier plate 4, and the carrier plate 4 is driven to the support frame 2 by a driving mechanism;

[0055] S2: stimulating the zebrafish fry in the porous plate 5 by the stimulation mechanism and collecting the motion trajectory data of the zebrafish fry by the infrared camera 14 and sending the motion trajectory data to the animal behavior trajectory tracking system for analysis and processing;

[0056] Among them, after the tracking and monitoring work is completed, when the carrying plate 4 is driven to move to the preset position by the driving mechanism, the zebrafish fry located in the porous plate 5 is sucked by the auxiliary mechanism 9, and then the zebrafish fry is transferred to an external placement container by the auxiliary mechanism 9. After the zebrafish fry is transferred to the external placement container, the carrying plate 4 is driven to reset to the support frame 2 by the driving mechanism, and then the two groups of box bodies 11 are driven to contact each other by the telescopic device 3 12, and then the disinfectant liquid is supplied by the disinfectant supply device, and the disinfectant liquid enters the diverter plate 15 and is then sprayed out from the nozzle to clean and disinfect the porous plate 5. After disinfection, an airflow with a certain amount of heat is supplied by the drying device to dry the porous plate 5.

[0057] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A fish stimulus response behavior tracking and monitoring device, comprising an observation box and an animal behavior trajectory tracking system connected to the observation box, wherein: The animal behavior trajectory tracking system is pre-configured in a virtual environment and is used to receive and analyze the fish behavior trajectory data collected by an observation box. The system is characterized in that: the observation box comprises a hollow box body (1) on one side, a support frame (2) is provided in the box body (1), a carrying plate (4) is attached to the top of the support frame (2), a porous plate (5) for placing fish is inserted on the carrying plate (4), the lower end of the porous plate (5) extends into the support frame (2), a stimulation mechanism for stimulating the fish in the porous plate (5) is provided in the support frame (2), an infrared camera (14) is provided on the inner wall of the box body (1) above the support frame (2), the carrying plate (4) is movably arranged in the box body (1) through a driving mechanism, an auxiliary mechanism (9) is provided on the inner wall of the top of the box body (1) and located outside the infrared camera (14), and the auxiliary mechanism (9) is used to collect the fish in the porous plate (5) when the driving mechanism drives the carrying plate (4) to move to a preset position.

2. The device according to claim 1, characterized in that: The stimulation mechanism comprises a light stimulation unit, a sound stimulation unit and a vibration stimulation unit; it also comprises a water supply pipe (3) having one end connected to the inner cavity of the support frame (2) and the other end connected to an external water supply device, and a heater arranged in the support frame (2); the support frame (2) is also connected to an external pump body via a drainage pipeline.

3. The device according to claim 1, characterized in that: The driving mechanism comprises: Two groups of movable bases (7) are respectively located at two ends of the bottom of the carrying plate (4) and are slidably connected to the inner wall of the bottom of the box (1); the two groups of movable bases (7) are respectively threadedly sleeved on the outer walls of two groups of screw rods (6); the screw rods (6) are longitudinally rotatably arranged in the box (1), and one end of the screw rod (6) movably penetrates the box (1) and is connected to the output end of the rotating device; and, Two sets of telescopic devices (8) are respectively arranged at two ends of the top of the bearing plate (4), and the output ends of the two sets of telescopic devices (8) both penetrate the bearing plate (4) and are respectively connected to the two sets of movable bases (7).

4. The device according to claim 1, characterized in that: The auxiliary mechanism (9) comprises: A top plate (91) is detachably mounted on the top inner wall of the box body (1); the bottom of the top plate (91) is connected to a bottom plate (92) via a connecting rod; a plurality of groups of adsorption cylinders (93) are detachably mounted on the bottom plate (92); the lower end of the adsorption cylinder (93) is connected to an adsorption head (94) for entering the hole of the porous plate (5); a piston is mounted in the adsorption cylinder (93); and the piston penetrates the top end of the adsorption cylinder (93) via a piston rod; and The second telescopic device (96) is arranged at the bottom of the top plate (91), and a movable plate (95) is arranged at the output end of the second telescopic device (96), and the movable plate (95) is detachably connected to the piston rod.

5. The device according to claim 1, characterized in that: The porous plate (5) has a T-shaped vertical cross section, and a T-shaped opening for placing the porous plate (5) is provided on the carrier plate (4). The length of the carrier plate (4) is greater than the length of the support frame (2) and less than the length of the inner cavity of the box cover (101). Box bodies (11) are symmetrically slidably provided on both sides of the top of the carrier plate (4). The opposite sides and the bottom of the two groups of the box bodies (11) are hollow. The opposite sides of the two groups of the box bodies (11) are both in contact with the output end of a telescopic device (12). The telescopic device (12) is fixedly arranged on the inner wall of the box body (1) and is used to drive the two groups of the box bodies (11) to contact each other. The opposite side of the box body (11) is also connected to the top of the carrier plate (4) through an elastic member (13) for driving the box body (11) to reset. The top inner walls of the two groups of box bodies (11) are both provided with a diverter plate (15). The bottom of the diverter plate (15) is provided with a plurality of groups of nozzles. A connecting pipe (17) is provided on the side of one of the diverter plates (15) close to the porous plate (5), and a docking hole for inserting the connecting pipe (17) is provided on the side of the other diverter plate (15) close to the porous plate (5). One of the diverter plates (15) is connected to an external drying device and a disinfectant supply device through a circulation pipeline (16).

6. The device according to claim 5, characterized in that: The inner walls of the two groups of the box bodies (11) are both vertically provided with electric guide rail mechanisms (18); a slider of the electric guide rail mechanism (18) is horizontally rotatably provided with an elastic telescopic rod (19); the other end of the elastic telescopic rod (19) is provided with a pressing plate (22) for contacting the porous plate (5); a gear (21) is sleeved on the outer wall of the fixed section of the elastic telescopic rod (19); a rack (20) is provided on the guide rail of the electric guide rail mechanism (18) for driving the gear (21) to drive the elastic telescopic rod (19) to rotate 180 degrees when the slider drives the elastic telescopic rod (19) upward to a preset position.

7. The device according to claim 1, characterized in that: The inner wall of the box (1) is provided with a lens frame which is rotated by a rotator, and a lens body (10) for covering the top of the porous plate (5) is embedded in the lens frame.

8. The device according to claim 1, characterized in that: One side of the box body (1) is provided with a box cover (101) for closing the hollow side thereof.

9. A method for tracking and monitoring fish stimulus response behavior, using the device according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1: When in use, a plurality of zebrafish fry that have been continuously exposed to CYN exposure solutions of different concentrations for a preset time are placed in a porous plate (5) containing embryo culture water, the porous plate (5) is placed on a carrier plate (4), and the carrier plate (4) is driven to the support frame (2) by a driving mechanism; S2: stimulating the zebrafish fry in the porous plate (5) through the stimulation mechanism and collecting the movement trajectory data of the zebrafish fry through the infrared camera (14) and sending the movement trajectory data to the animal behavior trajectory tracking system for analysis and processing; After the tracking and monitoring work is completed, when the carrying plate (4) is driven by the driving mechanism to move to a preset position, the zebrafish fry located in the porous plate (5) is sucked by the auxiliary mechanism (9), and then the zebrafish fry is transferred to an external placement container by the auxiliary mechanism (9).

Images