Antibacterial flexible patch and sterilization method

Abstract

The invention provides an antibacterial flexible patch and a sterilization method, and relates to the technical field of antibiosis, the antibacterial flexible patch comprises a hydrogel patch, a power supply and a controller, a plurality of through holes are formed in the hydrogel patch, the axial direction of the through holes is consistent with the thickness direction of the hydrogel patch, the through holes are filled with liquid metal, and the liquid metal is arranged in the hydrogel patch. The liquid metal is eutectic gallium indium alloy; the controller is electrically connected with the power supply; and the power supply is used for applying current to the liquid metal. When the antibacterial flexible patch provided by the invention is used, the controller is used for controlling the magnitude of current applied to the liquid metal by the power supply, so that the concentration of Ga < 3 + > released by the liquid metal is controlled, and rapid sterilization and rapid repair of tissues can be realized.

Description

Technical Field

[0001] The present invention relates to the field of antibacterial technology, and in particular to an antibacterial flexible patch and a sterilization method. Background Art

[0002] Liquid metal has excellent fluidity, conductivity and good biocompatibility, so it has attracted the attention of a large number of researchers. Relevant researchers have begun to apply liquid metal to wound tissue repair, which involves a sterilization stage and a tissue repair stage, that is, tissue repair is performed after sterilization. The sterilization speed and the tissue repair speed jointly determine the wound tissue repair speed. However, at present, liquid metal-based wound tissue repair products are still difficult to have both the characteristics of rapid sterilization and rapid tissue repair. Summary of the invention

[0003] The problem solved by the present invention is: how to achieve rapid sterilization and rapid tissue repair in a liquid metal-based wound tissue repair product.

[0004] To solve the above problems, the present invention provides an antibacterial flexible patch, including a hydrogel patch, a power supply and a controller, wherein a plurality of through holes are formed on the hydrogel patch, the axial direction of the through holes is consistent with the thickness direction of the hydrogel patch, the through holes are filled with liquid metal, and the liquid metal is a eutectic gallium-indium alloy; the controller is electrically connected to the power supply; and the power supply is used to apply current to the liquid metal.

[0005] Optionally, the material of the hydrogel patch is a composite hydrogel, and the composite hydrogel is formed by mixing tannic acid and hydrogel at a preset temperature.

[0006] Optionally, the preset temperature is 45-55°C

[0007] Optionally, the hydrogel is methacrylated gelatin.

[0008] Optionally, the mass fraction of the tannic acid in the composite hydrogel is 1-5%.

[0009] Optionally, the thickness of the hydrogel patch is 1-3 mm, and the inner diameter of the through hole is 0.1-1 mm.

[0010] Optionally, the plurality of through holes are distributed in an array on the hydrogel patch.

[0011] Optionally, the power supply is an alternating current power supply.

[0012] Optionally, the antibacterial flexible patch further includes a temperature sensor, and the temperature sensor is electrically connected to the controller.

[0013] Compared with the prior art, the antibacterial flexible patch provided by the present invention is applied to the wound when in use. In the sterilization stage, a controller controls the power supply to apply a large current (3-4 mA) to the liquid metal. Under the stimulation of the large current, the liquid metal releases more Ga 3+ , thus achieving rapid sterilization; in the tissue repair stage, the controller controls the power supply to apply a small current (0.9-1.1mA) to the liquid metal. Under the stimulation of the small current, the liquid metal releases less Ga 3+ , a small amount of Ga 3+ It can significantly promote rapid cell migration, thereby enabling rapid tissue repair. In addition, hydrogels can absorb exudate from wounds, promote skin breathing, and help skin healing.

[0014] The present invention also provides a sterilization method, based on the antibacterial flexible patch as described above, when the antibacterial flexible patch is used for sterilization, a preset current is applied to the liquid metal, and the magnitude of the preset current is 3-4 mA.

[0015] Compared with the prior art, the sterilization method provided by the present invention can achieve rapid sterilization and lay a foundation for the rapid repair of wound tissue. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of an antibacterial flexible patch in an embodiment of the present invention;

[0017] Figure 2 This is a scanning electron microscope image of methacrylic anhydride gelatin;

[0018] Figure 3 is a scanning electron microscope image of the composite hydrogel in Example 1;

[0019] Figure 4 is a scanning electron microscope image of the composite hydrogel in Example 2;

[0020] Figure 5 This is a scanning electron microscope image of the composite hydrogel in Example 3

[0021] Figure 6 This is a comparison chart of the adhesion strength between methacrylic anhydride gelatin and the composite hydrogels in Examples 1-3 and skin;

[0022] Figure 7 It is a comparison chart of the activity of Staphylococcus aureus in the experimental group 1-3 and the control group 1-2;

[0023] Figure 8 It is a comparison chart of the number of Staphylococcus aureus in the experimental group 1-3 and the control group 1-2;

[0024] Fig. 9The figure shows a comparison of the wound healing status of mice in the experimental group 1 and the control group 1 at different time periods.

[0025] Description of reference numerals:

[0026] 1. Hydrogel patch; 2. Liquid metal. DETAILED DESCRIPTION

[0027] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. Although certain embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be interpreted as being limited to the embodiments described herein. On the contrary, these embodiments are provided to provide a more thorough and complete understanding of the present invention. It should be understood that the drawings and embodiments of the present invention are only for exemplary purposes and are not intended to limit the scope of protection of the present invention.

[0028] Unless otherwise defined, all technical and scientific terms used in this application have the same meanings as those commonly understood by those skilled in the art to which this application belongs; the terms used in the specification of this application are only for the purpose of describing specific implementation methods and are not intended to limit this application;

[0029] The term "including" and its variations used in this article are open inclusions, that is, "including but not limited to"; the term "based on" is "based at least in part on"; the term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one other embodiment"; the term "some embodiments" means "at least some embodiments"; the term "optionally" means "optional embodiments". The relevant definitions of other terms will be given in the following description. It should be noted that the concepts of "first" and "second" mentioned in the present invention are used to distinguish different objects, rather than to describe a specific order or a primary and secondary relationship. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, the meaning of "multiple" is two or more.

[0030] like Figure 1As shown, an antibacterial flexible patch provided by an embodiment of the present invention includes a hydrogel patch 1, a power supply (not shown in the figure) and a controller (not shown in the figure), wherein a plurality of through holes are formed on the hydrogel patch 1, wherein the axial direction of the through holes is consistent with the thickness direction of the hydrogel patch 1, and the through holes are filled with liquid metal 2, wherein the liquid metal 2 is a eutectic gallium-indium alloy (EGaIn); the controller is electrically connected to the power supply; and the power supply is used to apply current to the liquid metal 2. Exemplarily, the positive electrode of the power supply is connected to the hydrogel patch 1, and the negative electrode of the power supply is respectively connected to the liquid metal 2 in each through hole.

[0031] The antibacterial flexible patch provided by the embodiment of the present invention is applied to the wound when in use. In the sterilization stage, the controller controls the power supply to apply a large current (3-4 mA) to the liquid metal 2. Under the stimulation of the large current, the liquid metal 2 releases more Ga 3+ , so that rapid sterilization can be achieved; in the tissue repair stage, the controller controls the power supply to apply a small current (0.9-1.1mA) to the liquid metal 2. Under the stimulation of the small current, the liquid metal 2 releases less Ga 3+ , a small amount of Ga 3+ It can significantly promote rapid cell migration, thereby enabling rapid tissue repair. In addition, hydrogels can absorb exudate from wounds, promote skin breathing, and help skin healing.

[0032] It should be noted that Ga 3+ The antibacterial mechanism is as follows: There is an iron carrier protein inside the bacteria, which is mainly responsible for transferring external Fe 3+ Transported to the interior of the bacteria. Under the attack of NADPH protein produced by mitochondria, Fe 3+ It will automatically detach and decompose inside the bacteria, providing the bacteria with the substances they need to proliferate. However, this iron carrier protein will mistakenly transfer Ga 3+ Identified as Fe 3+ So, Ga 3+ is transported into the bacteria. When attacked by NADPH proteins, Ga 3+ They do not automatically escape the control of iron carrier proteins. Instead, they accumulate in large quantities in bacteria. Due to the lack of necessary elements, the bacterial membrane ruptures, eventually leading to rapid bacterial apoptosis.

[0033] In some embodiments of the present invention, the material of the hydrogel patch 1 is a composite hydrogel, which is formed by mixing tannic acid and hydrogel at a preset temperature; the preset temperature is 45-55°C; the hydrogel is methacrylic anhydride gelatin (GelMA); the mass fraction of tannic acid in the composite hydrogel is 1-5%. Compared with hydrogel, the porosity of the composite hydrogel is lower, and as the content of tannic acid in the composite hydrogel increases, the porosity of the composite hydrogel gradually decreases, which means that Ga 3+ The resistance to free movement increases; compared with hydrogel, the composite hydrogel has a higher bonding strength with the skin, which means that the composite hydrogel has a better bonding effect with the skin, and as the tannic acid content in the composite hydrogel increases, the bonding strength between the composite hydrogel and the skin gradually increases. In order to balance the bonding strength of the composite hydrogel with the skin and Ga 3+ The easy release of ions, preferably, the mass fraction of the tannic acid in the composite hydrogel is 3%.

[0034] In some embodiments of the present invention, the thickness of the hydrogel patch 1 is 1-3 mm, and the inner diameter of the through hole is 0.1-1 mm. Since the surface tension of liquid metal is large and the inner diameter of the through hole is small, it can be ensured that the liquid metal will not flow out of the through hole.

[0035] In some embodiments of the present invention, a plurality of through holes are distributed in an array on the hydrogel patch 1; thereby, the Ga 3+ Even release at the wound site.

[0036] In some embodiments of the present invention, the power source is an AC power source. Compared with using a DC power source, using an AC power source can effectively prevent the adverse effects of water electrolysis on the antibacterial flexible patch.

[0037] In some embodiments of the present invention, the antibacterial flexible patch further comprises a temperature sensor, which is electrically connected to the controller. The increase in wound temperature is considered to be an important basis for determining wound status information such as wound bacterial infection or wound inflammation, so the wound status can be obtained by monitoring the wound temperature through the temperature sensor.

[0038] The embodiment of the present invention further provides a sterilization method, based on the antibacterial flexible patch as described above, when the antibacterial flexible patch is used for sterilization, a preset current is applied to the liquid metal 2, and the magnitude of the preset current is 3-4 mA.

[0039] The sterilization method provided by the embodiment of the present invention can achieve rapid sterilization and lay a foundation for rapid repair of wound tissue.

[0040] The present invention is further described below in conjunction with specific embodiments.

[0041] Example 1

[0042] An antibacterial flexible patch comprises a hydrogel patch, a power supply and a controller, wherein three through holes are formed on the hydrogel patch, wherein the axial direction of the through holes is consistent with the thickness direction of the hydrogel patch, and the through holes are filled with liquid metal, wherein the liquid metal is a eutectic gallium-indium alloy (EGaIn); the controller is electrically connected to the power supply; and the power supply is used to apply current to the liquid metal; wherein the hydrogel patch is cylindrical in shape, has a thickness of 2 mm, and an inner diameter of the through hole is 0.2 mm; the material of the hydrogel patch is a composite hydrogel, wherein the composite hydrogel is formed by mixing tannic acid and hydrogel at 50° C., the hydrogel is methacrylic anhydride-treated gelatin, and the mass fraction of tannic acid in the composite hydrogel is 1%.

[0043] Example 2

[0044] The difference from Example 1 is that the mass fraction of tannic acid in the composite hydrogel is 3%.

[0045] Example 3

[0046] The difference from Example 1 is that the mass fraction of tannic acid in the composite hydrogel is 5%.

[0047] Experimental Example 1

[0048] The methacrylic anhydride gelatin and the composite hydrogels in Examples 1-3 were characterized by scanning electron microscopy. Figure 2-5 ,from Figure 2-5 It can be seen that as the tannic acid content in the composite hydrogel increases, the porosity of the composite hydrogel gradually decreases. The adhesion strength between the methacrylic anhydride gelatin and the composite hydrogels in Examples 1-3 and the skin was tested, and the results are shown in FIG. Figure 6 ,from Figure 6 It can be seen that with the increase of tannic acid content in the composite hydrogel, the adhesion strength between the composite hydrogel and the skin gradually increased. Figure 6 0% TA, 1% TA, 3% TA, and 5% TA correspond to the experimental data of methacrylic anhydride gelatin and the composite hydrogel in Examples 1-3, respectively.

[0049] Experimental Example 2: In vitro antibacterial test

[0050] Experimental Group 1

[0051] The antibacterial flexible patch prepared in Example 2 was placed in a Staphylococcus aureus culture solution, and a current of 4 mA was applied to the liquid metal for 10 minutes.

[0052] Experimental Group 2

[0053] The antibacterial flexible patch prepared in Example 2 was placed in a Staphylococcus aureus culture solution, and a current of 3 mA was applied to the liquid metal for 10 minutes.

[0054] Experimental Group 3

[0055] The antibacterial flexible patch prepared in Example 2 was placed in a Staphylococcus aureus culture solution, and a current of 2 mA was applied to the liquid metal for 10 minutes.

[0056] Control group 1

[0057] The antibacterial flexible patch prepared in Example 2 was placed in a Staphylococcus aureus culture solution and kept for 10 minutes.

[0058] Control group 2

[0059] The Staphylococcus aureus culture solution was allowed to stand for 10 min.

[0060] The activity and number of Staphylococcus aureus in the experimental groups 1-3 and the control groups 1-2 were statistically analyzed. Figure 7 and 8 ;from Figure 7 and Figure 8 It can be seen that the activity and number of bacteria in experimental group 1-2 are both lower, indicating that the sterilization speed in experimental group 1-2 is faster and better. Figure 7 and Figure 8 In the figure, from right to left they correspond to the statistical results of experimental group 1, experimental group 2, experimental group 3, control group 1 and control group 2 respectively.

[0061] Experimental Example 3: Wound healing experiment

[0062] Experimental Group

[0063] After the wounds of infected wound model mice were subjected to the same sterilization treatment, the antibacterial flexible patch prepared in Example 2 was attached to the wound, a current of 1 mA was applied to the liquid metal for 10 min, and the healing of the wound was observed for the next 14 days.

[0064] Control group

[0065] After the wounds of infected wound model mice were subjected to the same sterilization treatment, the antibacterial flexible patch prepared in Example 2 was attached to the wounds, and the healing of the wounds was observed for the next 14 days.

[0066] Comparison of wound healing in mice at different time periods between experimental group 1 and control group 1 Fig. 9 ,from Fig. 9 It can be seen that compared with the control group, the wound healing of mice in the experimental group was better after 7 days; compared with the control group, the wound healing of mice in the experimental group was better after 14 days. Fig. 9The upper, middle, and lower pictures on the left side are pictures of the wounds of mice in the control group at 0 days, 7 days, and 14 days, respectively. Fig. 9 The upper, middle and lower three pictures on the middle right are pictures of the wounds of mice in the experimental group at 0 days, 7 days and 14 days respectively.

[0067] Although the present invention is disclosed as above, the protection scope of the present invention is not limited thereto. Those skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications will fall within the protection scope of the present invention.

Claims

1. An antibacterial flexible patch, characterized in that: The invention comprises a hydrogel patch (1), a power supply and a controller, wherein a plurality of through holes are formed on the hydrogel patch (1), wherein the axial direction of the through holes is consistent with the thickness direction of the hydrogel patch (1), and the through holes are filled with liquid metal (2), wherein the liquid metal (2) is a eutectic gallium-indium alloy; the controller is electrically connected to the power supply; and the power supply is used to apply current to the liquid metal (2).

2. The antibacterial flexible patch according to claim 1, characterized in that: The material of the hydrogel patch (1) is a composite hydrogel, and the composite hydrogel is formed by mixing tannic acid and hydrogel at a preset temperature.

3. The antibacterial flexible patch according to claim 2, characterized in that: The preset temperature is 45-55°C.

4. The antibacterial flexible patch according to claim 2, characterized in that: The hydrogel is methacrylic anhydride-treated gelatin.

5. The antibacterial flexible patch according to claim 4, characterized in that: The mass fraction of the tannic acid in the composite hydrogel is 1-5%.

6. The antibacterial flexible patch according to claim 1, characterized in that: The thickness of the hydrogel patch (1) is 1-3 mm, and the inner diameter of the through hole is 0.1-1 mm.

7. The antibacterial flexible patch according to claim 6, characterized in that: The plurality of through holes are distributed in an array on the hydrogel patch (1).

8. The antibacterial flexible patch according to claim 1, characterized in that: The power supply is an alternating current power supply.

9. The antibacterial flexible patch according to claim 1, characterized in that: A temperature sensor is also included, and the temperature sensor is electrically connected to the controller.

10. A sterilization method, based on the antibacterial flexible patch according to any one of claims 1 to 9, characterized in that: When the antibacterial flexible patch is used for sterilization, a preset current is applied to the liquid metal (2), and the magnitude of the preset current is 3-4 mA.

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