Antibacterial healing-promoting chitosan functional dressing, antibacterial healing-promoting functional solution as well as preparation method and application of antibacterial healing-promoting chitosan functional dressing and antibacterial healing-promoting functional solution

Abstract

The invention belongs to the technical field of medical dressings, and relates to an antibacterial healing-promoting chitosan functional dressing, an antibacterial healing-promoting functional solution as well as a preparation method and application of the antibacterial healing-promoting chitosan functional dressing. The antibacterial and healing-promoting functional solution comprises the following components in parts by weight: 6-8 parts of an ethanol aqueous solution containing organic acid and polyethylene glycol, 1-2 parts of a gelatin aqueous solution containing a healing-promoting component and 1-2 parts of an ethanol aqueous solution containing an antibacterial component, the gelatin aqueous solution containing the healing promoting component contains a recombinant human epidermal growth factor rhEGF and phenolic acid, and the ethanol aqueous solution containing the antibacterial component contains polyhexamethylene biguanide hydrochloride and polyethylene glycol dicarboxylic acid. The antibacterial and healing-promoting functional solution is loaded on chitosan base cloth to obtain the antibacterial and healing-promoting chitosan functional dressing, and the dressing has the gel characteristic, does not adhere to a wound, is continuously antibacterial, promotes wound healing, has excellent seepage management performance and can be widely applied to wound nursing.

Description

Technical Field

[0001] The invention belongs to the technical field of medical dressings, and relates to an antibacterial and healing-promoting chitosan functional dressing, an antibacterial and healing-promoting functional solution, and a preparation method and application thereof. Background Art

[0002] In the process of wound healing, bacterial infection is the main factor that hinders wound repair and healing. For wound repair under special conditions such as battlefield / traffic accident trauma, burns and scalds, and postoperative trauma, traditional wound dressings such as gauze and bandages mainly provide physical coverage of the wound, which can easily cause secondary infection and tissue damage to the wound. Studies have shown that keeping the wound surface in a moist environment can stimulate cell proliferation, accelerate epidermal migration, enhance white blood cell function, facilitate wound repair, and relieve pain. When treating clinical patient wounds, ideal wound dressings need to inhibit exogenous microorganisms and prevent bacterial growth, maintain a controllable moist environment, absorb exudate, allow gas exchange and promote fluid discharge, have a soft and flexible texture, and have biocompatibility and a certain strength.

[0003] Chitosan is a natural antibacterial material with advantages such as good biocompatibility and biodegradability. Its molecular structure is similar to that of cellulose and can be spun into non-woven fabrics. Chitosan dressings made from chitosan are soft in texture and can absorb wound exudate, keep the wound surface moist, and promote wound healing. The amino groups in chitosan molecules act as positive charge centers and can interact with the negative charges on the surface of bacteria to inhibit bacterial growth. However, due to the complex environment of the wound surface, the antibacterial sites on the surface of chitosan dressings are easily covered by bacteria and inflammatory factors. In addition, the pH of bacterially infected wounds is weakly alkaline, and chitosan is a cationic polymer. It only has antibacterial properties after the amino groups are protonated in an acidic environment. Therefore, in clinical use, the continuous antibacterial effect of chitosan dressings is not ideal.

[0004] Patent document CN110772660A proposes a method for preparing a guanidated chitosan dressing, firstly guanidating chitosan to enhance its antibacterial activity, then dissolving, filtering, degassing, spinning, washing, stretching and drying a certain mass ratio of guanidated chitosan by a wet spinning process to obtain guanidated chitosan fiber, and then preparing a guanidated chitosan non-woven dressing through opening, combing, laying and needle punching reinforcement processes. Its antibacterial performance is better than that of pure chitosan dressings prepared by the same method. However, this guanidation method is cumbersome and complicated, and the dressing after spinning only has antibacterial function on the surface, resulting in a waste of raw materials. At the same time, a high grafting rate will affect the mechanical properties of chitosan fiber. On the other hand, the guanidated chitosan prepared by the guanidated chitosan dressing is a monoguanidine, which still belongs to contact antibacterial, and the antibacterial site will also be covered by killed bacteria, weakening the antibacterial effect of the dressing.

[0005] Patent document CN113244437A discloses a preparation method of a biomimetic multifunctional composite sponge dressing, with hyaluronic acid and gelatin grafted with dopamine as composite dressing matrix, then the composite dressing matrix is ​​immersed in polyhexamethylene biguanide solution (PHMB), and the composite sponge dressing (DHGT+PHMB) loaded with polyhexamethylene biguanide is obtained by drying after ultrasonic vibration. Antibacterial experiments show that DHGT+PHMB has a strong inhibitory effect on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. However, this method directly immerses the composite dressing matrix in PHMB anhydrous ethanol solution, utilizes ultrasonic dispersion into matrix, utilizes the porosity of hydrogel to adsorb PHMB, and can cause PHMB to be released in large quantities in the early stage during use, which is far less than directly adding PHMB to the composite dressing matrix precursor to carry out cross-linking reaction.

[0006] Patent document CN113797385A discloses a chitosan / polyethylene glycol hydrogel and a preparation method thereof, wherein the hydrogel is obtained by reacting a four-arm polyethylene glycol active ester with a primary amine in a chitosan molecule to generate an amide bond; the antibacterial properties of the hydrogel are enhanced by loading polyhexamethylene guanidine hydrochloride in the hydrogel material. This method utilizes the cross-linking effect of the four-arm polyethylene glycol active ester to load PHMB into the chitosan hydrogel. However, the mechanical properties of the hydrogel dressing prepared by this method are relatively poor, far from the strength of the spinning dressing. At the same time, the preparation steps of the hydrogel dressing are cumbersome, which is not conducive to industrial production.

[0007] Wound dressings are usually changed every 3-5 days in clinical use, so higher requirements are placed on their ability to absorb and retain wound exudate and their ability to continuously resist bacteria and promote healing. Chitosan dressings have great advantages in biocompatibility, but their healing and sustained antibacterial effects are insufficient. Currently, there is no research on enhancing the healing and sustained antibacterial effects of chitosan dressings. Summary of the invention

[0008] Based on this, the purpose of the present invention is to provide an antibacterial and healing-promoting functional solution, which can functionally modify chitosan dressings and give the chitosan dressings sustained antibacterial and healing-promoting functions.

[0009] The invention also provides a method for preparing an antibacterial and healing-promoting functional solution and an antibacterial and healing-promoting chitosan functional dressing, as well as application of the antibacterial and healing-promoting chitosan functional dressing in wound care.

[0010] To achieve the above object, the present invention adopts the following technical solutions: The invention provides an antibacterial and healing-promoting functional solution, comprising the following components in parts by weight: 6-8 parts of an ethanol aqueous solution containing an organic acid and polyethylene glycol, 1-2 parts of a gelatin aqueous solution containing a healing-promoting component, and 1-2 parts of an ethanol aqueous solution containing an antibacterial component; the gelatin aqueous solution containing the healing-promoting component contains a recombinant human epidermal growth factor (rhEGF) and phenolic acid, and the ethanol aqueous solution containing the antibacterial component contains polyhexamethylene biguanide hydrochloride and polyethylene glycol dicarboxylic acid.

[0011] Furthermore, the organic acid in the ethanol aqueous solution includes any one or more of formic acid, acetic acid, lactic acid and malonic acid, the polyethylene glycol includes PEG200, PEG400, PEG600 or PEG1000; the molecular weight of the gelatin is 50kDa-100kDa, and the phenolic acid includes tannic acid, gentisic acid or protocatechuic acid.

[0012] Furthermore, the amounts of organic acid and polyethylene glycol added to the ethanol aqueous solution are 1.5%-3% and 0.5%-2% of the mass of the ethanol aqueous solution, respectively; the amounts of rhEGF and phenolic acid added to the gelatin aqueous solution containing the healing-promoting component are 0.01%-0.05% and 0.5%-1% of the mass of the gelatin aqueous solution containing the healing-promoting component, respectively; the amounts of polyethylene glycol dicarboxylic acid and PHMB added to the ethanol aqueous solution containing the antibacterial component are 1%-5% and 0.5%-2% of the mass of the ethanol aqueous solution containing the antibacterial component, respectively.

[0013] The present invention further provides a method for preparing an antibacterial and healing-promoting functional solution, which is prepared by mixing the ethanol aqueous solution, the gelatin aqueous solution containing the healing-promoting component and the ethanol aqueous solution containing the antibacterial component in proportion.

[0014] The present invention further provides an application of an antibacterial and healing-promoting functional solution in the modification of chitosan dressings.

[0015] The present invention further provides an antibacterial and healing-promoting chitosan functional dressing, wherein the base fabric of the chitosan dressing contains the antibacterial and healing-promoting functional solution, the antibacterial components in the antibacterial and healing-promoting functional solution are uniformly loaded into the chitosan base fabric by covalent grafting, and the healing-promoting factors are uniformly loaded into the chitosan base fabric by double cross-linked network adsorption through a one-step reaction.

[0016] Furthermore, the base fabric is made of chitosan raw material through wet spinning and non-woven needle punching process; the deacetylation degree of the chitosan raw material is ≥95%, and the viscosity is 100-200mPa.s.

[0017] The present invention further provides a method for preparing an antibacterial and healing-promoting chitosan functional dressing, comprising the following steps: S1, base fabric preparation: chitosan raw materials are slurried to obtain slurry, and then spun, coagulated, stretched, and dried to obtain fibers; the fibers are then cut into short fibers, humidified, and opened to form a fiber web; the fiber web is needle-punched and shaped to obtain a base fabric; S2, loading the antibacterial and healing-promoting functional solution onto the base fabric, and drying the base fabric to obtain Furthermore, the slurry in S1 includes the following components in mass percentage: chitosan raw material 2%-4%, urea 0.5%-1%, propylene glycol 0.2%-0.8%, anhydrous ethanol 0.1%-0.5%, zinc acetate 0.5%-2%, and the remainder is acetic acid aqueous solution; the mass fraction of the acetic acid aqueous solution is 1-5%.

[0018] Furthermore, the coagulation bath in S1 is a mixed solution of 5%-15% by mass of sodium hydroxide aqueous solution and an equal mass of anhydrous ethanol, and the coagulation bath temperature is adjusted to 20°C-25°C; the stretching ratio is 1-2 times, and the drying temperature is 50°C-70°C; the short fiber length is 1cm-2cm, the humidification condition is humidification for 1h~2h under a regain of 20%-30%, and the setting temperature is 130°C-150°C.

[0019] Furthermore, the loading amount of the antibacterial and healing promoting functional solution on the base fabric in S2 is 2-2.5 L / m 2 The loading method includes spraying or dipping, preferably spraying. The drying temperature is set at 100°C-130°C, and the drying time is 15min-30min.

[0020] Finally, the present invention provides an application of an antibacterial and healing-promoting chitosan functional dressing in wound care. The dressing is suitable for the care of chronic moderate and severe exudative wounds: it can be used for pressure sores, lower extremity venous ulcers, diabetic foot ulcers, II degree burns, skin grafts and donor areas or traumatic wounds.

[0021] The beneficial effects of the present invention are: 1. The antibacterial and healing-promoting chitosan functional dressing provided by the present invention has a 4-day antibacterial effect on Staphylococcus aureus, Escherichia coli, Candida albicans and Pseudomonas aeruginosa, which can effectively promote wound healing and shorten the healing time to 12 days. It can effectively avoid the infection risk caused by wound exposure due to frequent dressing changes, and reduce the pain caused to patients by dressing changes.

[0022] 2. The antibacterial and healing-promoting chitosan functional dressing provided by the present invention has excellent liquid absorption and liquid retention properties, with a liquid absorption capacity of more than 20 times. After absorbing the exudate, it forms a soft, sticky and hydrophilic double-network gel, which can be in close contact with the wound bed, eliminate cavities, fill and support cavity wounds, and provide a moist healing environment for wound healing. At the same time, the adsorbed antibacterial components and recombinant human epidermal growth factor rhEGF enter the gel and are slowly released to the wound surface to achieve continuous antibacterial and healing-promoting effects.

[0023] 3. The preparation method of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention, after the chitosan base cloth is treated with the antibacterial and healing-promoting functional solution, polyethylene glycol dicarboxylic acid is used as a connecting molecule, and under acidic and high temperature conditions, the carboxyl groups at both ends of the polyethylene glycol dicarboxylic acid form amide bonds with the amino groups of chitosan and polyhexamethylene biguanide hydrochloride PHMB respectively, and the polyhexamethylene biguanide hydrochloride PHMB is covalently grafted to the chitosan base cloth to enhance the antibacterial properties of the chitosan base cloth body. The ungrafted polyhexamethylene biguanide hydrochloride PHMB is adsorbed on the fiber and sustained-released after absorbing the exudate. After the chitosan dressing is modified with polyhexamethylene biguanide hydrochloride PHMB, it has a more excellent antibacterial effect, ensuring that the dressing body still has an antibacterial effect after the antibacterial agent is completely released, avoiding the contamination of the dressing body by microorganisms, and overcoming the shortcomings of the existing chitosan dressings in clinical practice that they cannot be continuously antibacterial.

[0024] 4. The preparation method of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention comprises gelatin and phenolic acid substances in the antibacterial and healing-promoting functional solution. The phenolic acid contains a large amount of phenolic hydroxyl groups, which can cross-link gelatin and chitosan fibers. The double network structure can play a role in the physical sustained release of antibacterial components and recombinant human epidermal growth factor rhEGF, while further enhancing the mechanical properties and biocompatibility of the chitosan dressing.

[0025] 5. The method for preparing the antibacterial and healing-promoting chitosan functional dressing provided by the present invention can obtain the antibacterial and healing-promoting chitosan functional dressing by spraying the antibacterial and healing-promoting functional solution in one step, which greatly shortens the entire production time and saves manpower and material resources. It is not only applicable to the multifunctional modification of chitosan-based fabrics, but also to the modification of other hydrophilic fiber-based fabrics. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solution of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] Attached Figure 1 A sample of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention after drying; Attached Figure 2 A schematic diagram of the grafting principle of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention; Attached Figure 3 The infrared spectrum of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention; Attached Figure 4 The antibacterial and healing-promoting chitosan functional dressing and the chitosan base cloth provided by the present invention are in a state after absorbing liquid; Attached Figure 5 Demonstration of the liquid-locking ability of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention; Attached Figure 6 The release curve of rhEGF of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention; Attached Figure 7 A flow chart of the continuous antibacterial experiment of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention; Attached Figure 8 This is a graph showing the results of a sustained antibacterial experiment of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. The embodiments mentioned are all implemented on the premise of the technical solutions of the present invention, and detailed implementation processes are given, but it should be stated that the protection scope of the present invention is not limited to the following embodiments.

[0030] Example 1 An antibacterial and healing-promoting chitosan functional dressing S1: Preparation of chitosan-based fabric: Preparation of S101 chitosan fiber: Weigh 0.5kg of acetic acid and dissolve it in 49.5kg of purified water to prepare a 1% acetic acid solution; add 2kg of chitosan, 0.25kg of urea, 0.3kg of propylene glycol, 0.05kg of anhydrous ethanol, and 0.5kg of zinc acetate to 46.9kg of the above acetic acid aqueous solution, close the feed port and all valves on the stirring kettle, stir for 6 hours, and complete the slurry preparation. Slowly add 5kg of NaOH to 95kg of purified water, then add an equal amount of anhydrous ethanol to mix well and pour into the coagulation bath, and adjust the coagulation bath temperature to 20℃. The prepared spinning slurry is filtered, degassed, spun, and coagulated to obtain the primary fiber; the primary fiber is washed and stretched 1 times, and then dried at 60℃ to obtain chitosan fiber.

[0031] S102: The chitosan fibers obtained in S101 were cut into 1 cm short fibers, humidified for 2 h at a moisture regain of 20%, poured into a hopper, mixed and loosened to form a uniform fiber web, and then needle-punched, shaped at 140°C, and wound to prepare a chitosan-based fabric.

[0032] S2: Preparation of antibacterial and healing functional solution: S201: Prepare an ethanol aqueous solution containing an organic acid and polyethylene glycol: weigh 93 g of anhydrous ethanol and 7 g of purified water to prepare a 93% ethanol aqueous solution, add 2.1 g of acetic acid, 0.3 g of malonic acid and 1 g of PEG600 to 96.6 g of the above ethanol solution, stir to dissolve, and mix to obtain an ethanol aqueous solution.

[0033] S202: Prepare a gelatin aqueous solution containing healing-promoting ingredients: weigh 2 g of gelatin into 98 g of purified water, heat and stir at 60°C to dissolve, and after complete dissolution, add 0.03 g of rhEGF (manufacturer: Aladdin, model: rp185510, purity ≥95%) and 0.8 g of tannic acid respectively, mix well to obtain a gelatin aqueous solution containing healing-promoting ingredients.

[0034] S203: Ethanol aqueous solution containing antibacterial components: Weigh 93g of anhydrous ethanol and 7g of purified water to prepare a 93% ethanol aqueous solution, add 2g of polyethylene glycol dicarboxylic acid and 1g of PHMB into 97g of the above ethanol aqueous solution, stir to dissolve, and mix well to obtain an ethanol aqueous solution containing antibacterial components.

[0035] S204: uniformly mix the above-mentioned ethanol aqueous solution: the gelatin aqueous solution containing the healing-promoting component: the ethanol aqueous solution containing the antibacterial component in a mass ratio of 8:1:1 to obtain an antibacterial and healing-promoting functional solution.

[0036] S3: Preparation of chitosan dressing: Cut the chitosan base cloth into small pieces of 10 cm × 10 cm, use a spray device to spray the above-mentioned antibacterial and healing-promoting functional solution onto the surface of the chitosan base cloth at a spray volume of 20 mL per piece to make it completely soaked, put it into a 130°C oven, continue to treat for 18 minutes, and obtain the antibacterial and healing-promoting chitosan functional dressing after drying.

[0037] Example 2 An antibacterial and healing-promoting chitosan functional dressing S1: Preparation of chitosan-based fabric: Preparation of S101 chitosan fiber: Weigh 1.5kg of acetic acid and dissolve it in 48.5kg of purified water to prepare a 3% acetic acid solution; add 1kg chitosan, 0.3kg urea, 0.1kg propylene glycol, 0.05kg anhydrous ethanol, and 0.5kg zinc acetate to 49.05kg of the above acetic acid aqueous solution, close the feed port and all valves on the stirring kettle, stir for 7h, and complete the slurry preparation. Slowly add 10kg of NaOH to 90kg of purified water, then add an equal amount of anhydrous ethanol to mix well and pour into the coagulation bath, and adjust the coagulation bath temperature to 23℃. The prepared spinning slurry is filtered, degassed, spun, and coagulated to obtain the primary fiber; the primary fiber is washed and stretched 1.5 times, and then dried at 50℃ to obtain chitosan fiber.

[0038] S102: The chitosan fibers obtained in S101 were cut into 1.5 cm short fibers, humidified for 1 h at a moisture regain of 25%, poured into a hopper, mixed and loosened to form a uniform fiber web, and then needle-punched, shaped at 130°C, and wound to prepare a chitosan-based fabric.

[0039] S2: Preparation of antibacterial and healing functional solution: S201: Prepare an ethanol aqueous solution containing an organic acid and polyethylene glycol: weigh 91 g of anhydrous ethanol and 9 g of purified water to prepare a 91% ethanol aqueous solution, add 2.1 g of formic acid, 0.3 g of malonic acid and 0.5 g of PEG800 to 97.1 g of the above ethanol solution, stir to dissolve, and mix to obtain an ethanol aqueous solution.

[0040] S202: Prepare a gelatin aqueous solution containing healing-promoting ingredients: weigh 1 g of gelatin into 99 g of purified water, heat and stir at 60°C to dissolve, and after complete dissolution, add 0.01 g of rhEGF (manufacturer: Aladdin, model: rp185510, purity ≥95%) and 0.8 g of gentisic acid, respectively, mix well to obtain a gelatin aqueous solution containing healing-promoting ingredients.

[0041] S203: Ethanol aqueous solution containing antibacterial ingredients: weigh 95g of anhydrous ethanol and 5g of purified water to prepare a 95% ethanol aqueous solution, add 1g of polyethylene glycol dicarboxylic acid and 0.5g of PHMB to 98.5g of the above ethanol aqueous solution, stir to dissolve, and mix to obtain an ethanol aqueous solution containing antibacterial ingredients.

[0042] S204: Evenly mix the above-mentioned ethanol aqueous solution: the gelatin aqueous solution containing the healing-promoting component: the ethanol aqueous solution containing the antibacterial component in a mass ratio of 7:2:1 to obtain an antibacterial and healing-promoting functional solution.

[0043] S3: Preparation of chitosan dressing: Cut the chitosan base cloth into small pieces of 10 cm × 10 cm, use a spray device to spray the above-mentioned antibacterial and healing-promoting functional solution onto the surface of the chitosan base cloth at a spray volume of 23 mL per piece to make it completely soaked, put it into a 100°C oven, continue to treat for 30 minutes, and obtain the antibacterial and healing-promoting chitosan functional dressing after drying.

[0044] Example 3 An antibacterial and healing-promoting chitosan functional dressing S1: Preparation of chitosan-based fabric: Preparation of S101 chitosan fiber: Weigh 2.5kg of acetic acid and dissolve it in 47.5kg of purified water to prepare a 5% acetic acid solution; add 1.5kg chitosan, 0.25kg urea, 0.3kg propylene glycol, 0.1kg anhydrous ethanol, and 0.25kg zinc acetate to 47.6kg of the above acetic acid aqueous solution, close the feed port and all valves on the stirring kettle, stir for 8h, and complete the slurry preparation. Slowly add 15kg of NaOH to 85kg of purified water, then add an equal amount of anhydrous ethanol to mix well and pour into the coagulation bath, and adjust the coagulation bath temperature to 25℃. The prepared spinning slurry is filtered, degassed, spun, and coagulated to obtain primary fibers; the primary fibers are washed and stretched 2 times, and then dried at 70℃ to obtain chitosan fibers.

[0045] S102: The chitosan fibers obtained in S101 were cut into 2 cm short fibers, humidified for 1.5 h at a moisture regain of 30%, poured into a hopper, mixed and loosened to form a uniform fiber web, and then needle-punched, shaped at 150°C, and wound to prepare a chitosan-based fabric.

[0046] S2: Preparation of antibacterial and healing functional solution: S201: Prepare an ethanol aqueous solution containing an organic acid and polyethylene glycol: weigh 95 g of anhydrous ethanol and 5 g of purified water to prepare a 95% ethanol aqueous solution, add 1.5 g of acetic acid, 0.7 g of malonic acid and 1 g of PEG600 to 96.8 g of the above ethanol solution, stir to dissolve, and mix to obtain an ethanol aqueous solution.

[0047] S202: Prepare a gelatin aqueous solution containing healing-promoting ingredients: weigh 5 g of gelatin into 95 g of purified water, heat and stir at 60°C to dissolve, and after complete dissolution, add 0.05 g of rhEGF (manufacturer: Aladdin, model: rp185510, purity ≥95%) and 1 g of tannic acid respectively, mix well to obtain a gelatin aqueous solution containing healing-promoting ingredients.

[0048] S203: Ethanol aqueous solution containing antibacterial ingredients: Weigh 91g of anhydrous ethanol and 9g of purified water to prepare a 91% ethanol aqueous solution, add 2g of polyethylene glycol dicarboxylic acid and 2g of PHMB to 96g of the above ethanol aqueous solution, stir to dissolve, and mix to obtain an ethanol aqueous solution containing antibacterial ingredients.

[0049] S204: Evenly mix the above-mentioned ethanol aqueous solution: the gelatin aqueous solution containing the healing-promoting component: the ethanol aqueous solution containing the antibacterial component in a mass ratio of 6:2:2 to obtain an antibacterial and healing-promoting functional solution.

[0050] S3: Preparation of chitosan dressing: Cut the chitosan base cloth into small pieces of 10 cm × 10 cm, use a spray device to spray the above-mentioned antibacterial and healing-promoting functional solution onto the surface of the chitosan base cloth at a spray volume of 25 mL per piece to make it completely soaked, put it into a 120°C oven, continue to treat for 25 minutes, and obtain the antibacterial and healing-promoting chitosan functional dressing after drying.

[0051] Example 4 An antibacterial and healing-promoting chitosan functional dressing S1: Preparation of chitosan-based fabric: Preparation of S101 chitosan fiber: Weigh 0.5kg of acetic acid and dissolve it in 49.5kg of purified water to prepare a 1% acetic acid solution; add 1kg chitosan, 0.5kg urea, 0.4kg propylene glycol, 0.05kg anhydrous ethanol, and 0.25kg zinc acetate to 47.8kg of the above acetic acid aqueous solution, close the feed port and all valves on the stirring kettle, stir for 7h, and complete the slurry preparation. Slowly add 5kg of NaOH to 95kg of purified water, then add an equal amount of anhydrous ethanol to mix well and pour into the coagulation bath, and adjust the coagulation bath temperature to 23℃. The prepared spinning slurry is filtered, degassed, spun, and coagulated to obtain the primary fiber; the primary fiber is washed and stretched 1.5 times, and then dried at 60℃ to obtain chitosan fiber.

[0052] S102: The chitosan fibers obtained in S101 were cut into 1.5 cm short fibers, humidified for 2 h at a moisture regain of 20%, poured into a hopper, mixed and loosened to form a uniform fiber web, and then needle-punched, shaped at 130°C, and wound to prepare a chitosan-based fabric.

[0053] S2: Preparation of antibacterial and healing functional solution: S201: Prepare an ethanol aqueous solution containing an organic acid and polyethylene glycol: weigh 91 g of anhydrous ethanol and 9 g of purified water to prepare a 91% ethanol aqueous solution, add 1.5 g of lactic acid, 0.6 g of malonic acid and 2 g of PEG200 to 95.9 g of the above ethanol solution, stir to dissolve, and mix to obtain an ethanol aqueous solution.

[0054] S202: Prepare a gelatin aqueous solution containing healing-promoting ingredients: weigh 5 g of gelatin into 95 g of purified water, heat and stir at 60°C to dissolve, and after complete dissolution, add 0.03 g of rhEGF (manufacturer: Aladdin, model: rp185510, purity ≥95%) and 0.5 g of protocatechuic acid, respectively, and mix well to obtain a gelatin aqueous solution containing healing-promoting ingredients.

[0055] S203: Ethanol aqueous solution containing antibacterial components: weigh 95g of anhydrous ethanol and 5g of purified water to prepare a 95% ethanol aqueous solution, add 5g of polyethylene glycol dicarboxylic acid and 2g of PHMB to 93g of the above ethanol aqueous solution, stir to dissolve, and mix to obtain an ethanol aqueous solution containing antibacterial components.

[0056] S204: Evenly mix the above-mentioned ethanol aqueous solution: the gelatin aqueous solution containing the healing-promoting component: the ethanol aqueous solution containing the antibacterial component in a mass ratio of 7:1:2 to obtain an antibacterial and healing-promoting functional solution.

[0057] S3: Preparation of chitosan dressing: Cut the chitosan base cloth into small pieces of 10 cm × 10 cm, use a spray device to spray the above-mentioned antibacterial and healing-promoting functional solution onto the surface of the chitosan base cloth at a spray volume of 23 mL per piece to make it completely soaked, put it into a 120°C oven, continue to treat for 25 minutes, and obtain the antibacterial and healing-promoting chitosan functional dressing after drying.

[0058] Example 5 An antibacterial and healing-promoting chitosan functional dressing S1: Preparation of chitosan-based fabric: Preparation of S101 chitosan fiber: Weigh 2.5kg of acetic acid and dissolve it in 47.5kg of purified water to prepare a 5% acetic acid solution; add 2kg chitosan, 0.3kg urea, 0.1kg propylene glycol, 0.25kg anhydrous ethanol, and 1kg zinc acetate to 46.35kg of the above acetic acid aqueous solution, close the feed port and all valves on the stirring kettle, stir for 6 hours, and complete the slurry preparation. Slowly add 15kg of NaOH to 85kg of purified water, then add an equal amount of anhydrous ethanol to mix well and pour into the coagulation bath, and adjust the coagulation bath temperature to 20℃. The prepared spinning slurry is filtered, degassed, spun, and coagulated to obtain primary fibers; the primary fibers are washed and stretched 2 times, and then dried at 60℃ to obtain chitosan fibers.

[0059] S102: The chitosan fibers obtained in S101 were cut into 2 cm short fibers, humidified for 1.5 h at a moisture regain of 30%, poured into a hopper, mixed and loosened to form a uniform fiber web, and then needle-punched, shaped at 140°C, and wound to prepare a chitosan-based fabric.

[0060] S2: Preparation of antibacterial and healing functional solution: S201: Prepare an ethanol aqueous solution containing an organic acid and polyethylene glycol: weigh 95 g of anhydrous ethanol and 5 g of purified water to prepare a 95% ethanol aqueous solution, add 1.5 g of acetic acid, 0.4 g of malonic acid, 0.3 g of formic acid and 2 g of PEG400 to 95.8 g of the above ethanol solution, stir to dissolve, and mix to obtain an ethanol aqueous solution.

[0061] S202: Prepare a gelatin aqueous solution containing healing-promoting ingredients: weigh 2 g of gelatin into 98 g of purified water, heat and stir at 60°C to dissolve, and after complete dissolution, add 0.05 g of rhEGF (manufacturer: Aladdin, model: rp185510, purity ≥95%) and 0.5 g of gentisic acid, respectively, mix well to obtain a gelatin aqueous solution containing healing-promoting ingredients.

[0062] S203: Ethanol aqueous solution containing antibacterial ingredients: weigh 93g of anhydrous ethanol and 7g of purified water to prepare a 93% ethanol aqueous solution, add 2g of polyethylene glycol dicarboxylic acid and 0.5g of PHMB to 97.5g of the above ethanol aqueous solution, stir to dissolve, and mix to obtain an ethanol aqueous solution containing antibacterial ingredients.

[0063] S204: uniformly mix the above-mentioned ethanol aqueous solution: gelatin aqueous solution containing healing-promoting components: ethanol aqueous solution containing antibacterial components in a mass ratio of 6.5:2:1.5 to obtain an antibacterial and healing-promoting functional solution.

[0064] S3: Preparation of chitosan dressing: Cut the chitosan base cloth into small pieces of 10 cm × 10 cm, use a spray device to spray the above-mentioned antibacterial and healing-promoting functional solution onto the surface of the chitosan base cloth at a spray volume of 25 mL per piece to make it completely soaked, put it into a 100°C oven, continue to treat for 30 minutes, and obtain the antibacterial and healing-promoting chitosan functional dressing after drying.

[0065] Example 6 An antibacterial and healing-promoting chitosan functional dressing S1: Preparation of chitosan-based fabric: Preparation of S101 chitosan fiber: Weigh 1.5kg of acetic acid and dissolve it in 48.5kg of purified water to prepare a 3% acetic acid solution; add 1.5kg chitosan, 0.25kg urea, 0.1kg propylene glycol, 0.05kg anhydrous ethanol, and 0.25kg zinc acetate to 47.85kg of the above acetic acid aqueous solution, close the feed port and all valves on the stirring kettle, stir for 8h, and complete the slurry preparation. Slowly add 10kg of NaOH to 80kg of purified water, then add an equal amount of anhydrous ethanol to mix well and pour into the coagulation bath, and adjust the coagulation bath temperature to 25℃. The prepared spinning slurry is filtered, degassed, spun, and coagulated to obtain the primary fiber; the primary fiber is washed and stretched 1 times, and then dried at 50℃ to obtain chitosan fiber.

[0066] S102: The chitosan fibers obtained in S101 were cut into 1 cm short fibers, humidified for 1 h at a moisture regain of 25%, poured into a hopper, mixed and loosened to form a uniform fiber web, and then needle-punched, shaped at 150°C, and wound to prepare a chitosan-based fabric.

[0067] S2: Preparation of antibacterial and healing functional solution: S201: Prepare an ethanol aqueous solution containing an organic acid and polyethylene glycol: weigh 93 g of anhydrous ethanol and 7 g of purified water to prepare a 93% ethanol aqueous solution, add 1.5 g of lactic acid, 0.6 g of malonic acid and 1.5 g of PEG600 to 96.4 g of the above ethanol solution, stir to dissolve, and mix to obtain an ethanol aqueous solution.

[0068] S202: Prepare a gelatin aqueous solution containing healing-promoting ingredients: weigh 1 g of gelatin into 99 g of purified water, heat and stir at 60°C to dissolve, and after complete dissolution, add 0.03 g of rhEGF (manufacturer: Aladdin, model: rp185510, purity ≥95%) and 1 g of protocatechuic acid, respectively, mix well to obtain a gelatin aqueous solution containing healing-promoting ingredients.

[0069] S203: Ethanol aqueous solution containing antibacterial ingredients: Weigh 91g of anhydrous ethanol and 9g of purified water to prepare a 91% ethanol aqueous solution, add 5g of polyethylene glycol dicarboxylic acid and 1g of PHMB to 94g of the above ethanol aqueous solution, stir to dissolve, and mix to obtain an ethanol aqueous solution containing antibacterial ingredients.

[0070] S204: Evenly mix the above-mentioned ethanol aqueous solution: the gelatin aqueous solution containing the healing-promoting component: the ethanol aqueous solution containing the antibacterial component in a mass ratio of 7:1.5:1.5 to obtain an antibacterial and healing-promoting functional solution.

[0071] S3: Preparation of chitosan dressing: Cut the chitosan base cloth into small pieces of 10 cm × 10 cm, use a spray device to spray the above-mentioned antibacterial and healing-promoting functional solution onto the surface of the chitosan base cloth at a spray volume of 20 mL per piece to make it completely soaked, put it into a 100°C oven, continue to treat for 30 minutes, and obtain the antibacterial and healing-promoting chitosan functional dressing after drying.

[0072] Comparative Example 1 Chitosan dressing S1: Preparation of chitosan-based fabric: Preparation of S101 chitosan fiber: Weigh 0.5kg of acetic acid and dissolve it in 49.5kg of purified water to prepare a 1% acetic acid solution; add 2kg chitosan, 0.25kg urea, 0.3kg propylene glycol, 0.05kg anhydrous ethanol, and 0.5kg zinc acetate to 46.9kg of the above acetic acid aqueous solution, close the feed port and all valves on the stirring kettle, stir for 6 hours, and complete the slurry preparation. Slowly add 5kg of NaOH to 95kg of purified water, then add an equal amount of anhydrous ethanol to mix well and pour into the coagulation bath, and adjust the coagulation bath temperature to 20℃. The prepared spinning slurry is filtered, degassed, spun, and coagulated to obtain primary fibers; the primary fibers are washed and stretched 1 times, and then dried at 60℃ to obtain chitosan fibers.

[0073] S102: The chitosan fibers obtained in S101 were cut into 1 cm short fibers, humidified for 2 h at a moisture regain of 20%, poured into a hopper, mixed and loosened to form a uniform fiber web, and then needle-punched, shaped at 140°C, and wound to prepare a chitosan-based fabric.

[0074] S2: Weigh 93g of anhydrous ethanol and 7g of purified water to prepare a 93% ethanol-water solution.

[0075] S3: Preparation of chitosan dressing: Cut the chitosan base cloth into small pieces of 10 cm × 10 cm, use a spray device to spray the above ethanol aqueous solution onto the surface of the chitosan base cloth at a spray volume of 20 mL per piece to make it completely soaked, put it into a 130°C oven, continue to treat for 18 minutes, and obtain the chitosan dressing after drying.

[0076] Comparative Example 2 Chitosan dressing S1: Preparation of chitosan-based fabric: Preparation of S101 chitosan fiber: Weigh 0.5kg of acetic acid and dissolve it in 49.5kg of purified water to prepare a 1% acetic acid solution; add 2kg chitosan, 0.25kg urea, 0.3kg propylene glycol, 0.05kg anhydrous ethanol, and 0.5kg zinc acetate to 46.9kg of the above acetic acid aqueous solution, close the feed port and all valves on the stirring kettle, stir for 6 hours, and complete the slurry preparation. Slowly add 5kg of NaOH to 95kg of purified water, then add an equal amount of anhydrous ethanol to mix well and pour into the coagulation bath, and adjust the coagulation bath temperature to 20℃. The prepared spinning slurry is filtered, degassed, spun, and coagulated to obtain primary fibers; the primary fibers are washed and stretched 1 times, and then dried at 60℃ to obtain chitosan fibers.

[0077] S102: The chitosan fibers obtained in S101 were cut into 1 cm short fibers, humidified for 2 h at a moisture regain of 20%, poured into a hopper, mixed and loosened to form a uniform fiber web, and then needle-punched, shaped at 140°C, and wound to prepare a chitosan-based fabric.

[0078] S2: Preparation of ethanol aqueous solution: weigh 93 g of anhydrous ethanol and 7 g of purified water to prepare a 93% ethanol aqueous solution, add 2.1 g of acetic acid, 0.3 g of malonic acid and 1 g of PEG600 to 96.6 g of the above ethanol solution, stir to dissolve, and mix to obtain an ethanol aqueous solution.

[0079] S3: Preparation of chitosan dressing: Cut the chitosan base cloth into small pieces of 10 cm × 10 cm, use a spray device to spray the above ethanol aqueous solution onto the surface of the chitosan base cloth at a spray volume of 20 mL per piece to make it completely soaked, put it into a 130°C oven, continue to treat for 18 minutes, and obtain the chitosan dressing after drying.

[0080] Characterization and performance analysis 1. Material Characterization The dried sample of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention is as follows: Figure 1 shown.

[0081] The present invention provides a method for preparing an antibacterial and healing-promoting chitosan functional dressing. After the chitosan base cloth is treated with an antibacterial and healing-promoting functional solution, polyethylene glycol dicarboxylic acid (PEG-2COOH) is used as a connecting molecule. Under acidic and high temperature conditions, the carboxyl groups at both ends of PEG-2COOH form amide bonds with the amino groups of chitosan (CS) and polyhexamethylene biguanide hydrochloride (PHMB) respectively, and the PHMB is covalently grafted to the CS base cloth to enhance the antibacterial performance of the CS base cloth. The grafting process is as follows: Figure 2 shown.

[0082] The antibacterial and healing-promoting chitosan functional dressing in Example 1 was analyzed by infrared spectroscopy. The results are as follows: Figure 3 In the infrared spectrum of chitosan, 3290 cm -1 The broad peaks on the left and right are the stretching vibration absorption peaks of the OH of the hydroxyl group and the NH of the amino group after the formation of hydrogen bonds, 1660 cm -1 and 1587cm -1 It is the amino deformation vibration absorption peak in chitosan, 1059cm -1 The 1739cm-1 is the stretching vibration absorption peak of the hydroxyl group OH in chitosan that has not formed a hydrogen bond. -1 It is the C=O stretching vibration absorption peak of carboxylic acid. In the infrared spectrum of CS-PHMB, the 1660cm -1 and 1587cm-1 The amino deformation vibration absorption peak at 1739cm -1 The C=O stretching vibration absorption peak of carboxylic acid disappears at 1544cm -1 A new characteristic absorption peak that is not found in chitosan, PEG-2COOH, and PHMB appears at 1404 cm -1 A new characteristic absorption peak that is not found in chitosan, PEG-2COOH and PHMB appears at the position, which is attributed to the stretching vibration absorption peak of amide III (CN). This indicates that the carboxyl groups at both ends of PEG-2COOH undergo condensation reactions with the amino groups on the chitosan molecule and the amino groups on PHMB, respectively, to generate new chemical bonds, and PHMB is grafted onto the chitosan molecule through amide bonds.

[0083] 2. Liquid absorption, gelling properties, water vapor transmission rate The results are shown in Table 1 below, following the methods specified in 3.2, 3.5 and 3.2 of YY / T0471.1-2004. Figure 4 The states of the antibacterial and healing-promoting chitosan functional dressing and the chitosan base cloth after absorbing liquid provided by the present invention are given. Figure 5 The liquid-locking ability of the antibacterial and healing-promoting chitosan functional dressing provided by the present invention is demonstrated. The figure shows that different positions of the same dressing absorb liquids of different colors without color bleeding.

[0084] It can be seen from the results that the antibacterial and healing-promoting chitosan functional dressing provided by the present invention has excellent liquid absorption and liquid locking properties, with a liquid absorption capacity of more than 20 times. After absorbing the exudate, it forms a soft, sticky and hydrophilic double-network gel, which can be in close contact with the wound bed, eliminate the cavity, fill and support the cavity wound, and provide a moist healing environment for wound healing. At the same time, the adsorbed antibacterial components and recombinant human epidermal growth factor enter the gel and are slowly released to the wound surface to achieve a continuous antibacterial and healing-promoting effect.

[0085] 3. In vitro release curve test of recombinant human epidermal growth factor rhEGF The chitosan dressing prepared in Example 1 was used for this test. 0.3 g of chitosan dressing was weighed and placed in a centrifuge tube. 30 mL of normal saline was added. The centrifuge tube was placed on a constant temperature shaker and shaken at 37°C and 60 rpm. The centrifuge tube was taken out at 0 h, 4 h, 8 h, 12 h, 24 h, 48 h, 60 h, and 72 h, respectively. 100 uL of the supernatant was aspirated and 100 uL of normal saline was added. The amount of rhEGF in the supernatant was determined by the BCA method, and the cumulative release rate was calculated. The results are shown in Figure 2. Figure 6 shown.

[0086] The results show that the cumulative release of rhEGF was about 25% in the first 4 hours, and then slowly released, with the cumulative release reaching 92% in 72 hours. This sustained release can continuously stimulate epithelial cells and accelerate wound healing.

[0087] 4. Wound healing test The dressings prepared in Example 1 and Comparative Examples 1-2 were selected for wound healing test. 20 adult male SD rats were randomly divided into 4 groups, with 5 rats in each group. One group was the model group and the rest were experimental groups, and the experimental groups corresponded to Example 1 and Comparative Examples 1-2, respectively. The rats were fasted for 12 hours in advance, and anesthetized by intraperitoneal injection of 1% sodium pentobarbital aqueous solution at a dose of 35 mg / kg. After the rats entered the anesthetized state, the rats were fixed, the hair on the back of the rats was removed, and after disinfection, the full-thickness skin was removed at the exposed skin in a size of 2cm×2cm, deep to the fascia layer, to form a square wound to establish a rat skin damage model. Then the dressing corresponding to the group was used for treatment, and the model group was treated with gauze. The dressing corresponding to the group was changed every 2 days, and the wound healing was observed and recorded. The wound healing rates on the 4th, 8th, 12th, 16th and 20th days were calculated respectively. The wound healing effect was expressed as the wound healing rate, which was (initial wound area - unhealed wound area on the nth day) / initial wound area × 100%. The results are shown in Table 2 below.

[0088] It can be seen from the results that compared with the model group, the wound healing rate increased significantly after using the chitosan dressing of Example 1; compared with Comparative Examples 1 and 2, the wound healing rate increased significantly after using the chitosan dressing of Example 1, indicating that the human recombinant epidermal growth factor added to the chitosan dressing has the effect of stimulating epithelial cell proliferation and differentiation, promoting wound epithelialization and neovascularization, accelerating the wound healing process, and shortening the healing time to 12 days. It can effectively avoid the infection risk caused by frequent changes of dressings and wound exposure, and reduce the pain caused to patients by changing dressings. Comparative Example 1 has no difference in healing effect compared with the model group. The chitosan-based cloth dressing has not been modified and has no antibacterial properties, which is equivalent to the effect of ordinary gauze. Comparative Example 2 has a slightly improved healing rate compared with the model group. After chitosan is treated with an antibacterial and healing-promoting functional solution, the antibacterial property is slightly improved, which reduces the growth of bacteria on the surface of the wound and is conducive to the formation of scabs.

[0089] 5. Antibacterial and sustained antibacterial performance test Common wound bacteria species - Staphylococcus aureus, Escherichia coli, Candida albicans, and Pseudomonas aeruginosa were used as experimental bacteria, and the antibacterial test was carried out according to the method in Appendix C of GB 15979-2002. The results are shown in Table 3 below.

[0090] The results show that the chitosan dressing prepared by the present invention has a good in vitro antibacterial effect, and the antibacterial rates against Staphylococcus aureus, Escherichia coli, Candida albicans, and Pseudomonas aeruginosa can reach more than 99%. The antibacterial effect is significant and significantly better than that of the control group. It can effectively inhibit the reproduction of microorganisms in the wound bed and reduce the risk of wound infection.

[0091] The continuous antibacterial performance test was carried out by the following method: antibacterial chitosan dressing, ordinary chitosan dressing, and commercially available silver ion dressing were cut into 2.5 cm × 2.5 cm samples and irradiated for sterilization; nutrient agar corresponding to the test strain was prepared, poured into a plate after sterilization and solidified for use; the concentration of the prepared agar was 6 × 10 5 cfu / ml of the test bacterial suspension was evenly spread on the nutrient agar plate with a cotton swab; after adding physiological saline to the surface of the test sample, stick it on the culture medium and gently press it until it is completely attached. Then, after culturing in a 37°C biochemical incubator for 18 hours, take it out to observe the bacterial growth and the size of the inhibition zone, and take photos for record. Remove the dressing from the culture dish with tweezers and place it in another newly prepared culture dish containing bacteria and repeat the above experiment for 4 days. The experimental process is as follows Figure 7 As shown in Table 4 and Figure 8 The results are determined according to the requirements of GB / T 20944.1-2007 Evaluation of antimicrobial properties of textiles Part 1: Agar plate diffusion method 10.3 and 10.4 for determining the continuous antimicrobial time.

[0092] The results show that the chitosan dressing prepared by the present invention has a good in vitro sustained antibacterial effect, and the sustained antibacterial time for Staphylococcus aureus, Escherichia coli, Candida albicans, and Pseudomonas aeruginosa is up to 4 days, which can effectively avoid the risk of frequent dressing changes and the risk of microbial contamination of the dressing itself.

[0093] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An antibacterial and healing-promoting functional solution, characterized in that: The invention comprises the following components in parts by weight: 6-8 parts of an ethanol aqueous solution containing an organic acid and polyethylene glycol, 1-2 parts of a gelatin aqueous solution containing a healing-promoting component, and 1-2 parts of an ethanol aqueous solution containing an antibacterial component; the gelatin aqueous solution containing the healing-promoting component contains recombinant human epidermal growth factor (rhEGF) and phenolic acid, and the ethanol aqueous solution containing the antibacterial component contains polyhexamethylene biguanide hydrochloride and polyethylene glycol dicarboxylic acid.

2. The antibacterial and healing promoting functional solution according to claim 1, characterized in that: The organic acid in the ethanol aqueous solution includes any one or more of formic acid, acetic acid, lactic acid and malonic acid, the polyethylene glycol includes PEG200, PEG400, PEG600 or PEG1000; the molecular weight of the gelatin is 50kDa-100kDa, and the phenolic acid includes tannic acid, gentisic acid or protocatechuic acid.

3. The antibacterial and healing promoting functional solution according to claim 1, characterized in that: The added amounts of organic acid and polyethylene glycol in the ethanol aqueous solution are 1.5%-3% and 0.5%-2% of the mass of the ethanol aqueous solution, respectively; the added amounts of rhEGF and phenolic acid in the gelatin aqueous solution containing the healing-promoting component are 0.01%-0.05% and 0.5%-1% of the mass of the gelatin aqueous solution containing the healing-promoting component, respectively; the added amounts of polyethylene glycol dicarboxylic acid and polyhexamethylene biguanide hydrochloride in the ethanol aqueous solution containing the antibacterial component are 1%-5% and 0.5%-2% of the mass of the ethanol aqueous solution containing the antibacterial component, respectively.

4. A method for preparing the antibacterial and healing-promoting functional solution according to any one of claims 1 to 3, characterized in that: The ethanol aqueous solution, the gelatin aqueous solution containing the healing-promoting component and the ethanol aqueous solution containing the antibacterial component are mixed in proportion to obtain the product.

5. Use of the antibacterial and healing-promoting functional solution according to any one of claims 1 to 3 in the modification of chitosan dressings.

6. An antibacterial and healing-promoting chitosan functional dressing, characterized in that: The base fabric of the chitosan functional dressing contains the antibacterial and healing-promoting functional solution according to any one of claims 1 to 3.

7. The antibacterial and healing-promoting chitosan functional dressing according to claim 6, characterized in that: The base fabric is made of chitosan raw material through wet spinning and non-woven needle punching process; the deacetylation degree of the chitosan raw material is ≥95%, and the viscosity is 100-200mPa.s.

8. A method for preparing the antibacterial and healing-promoting chitosan functional dressing according to claim 6 or 7, characterized in that: The steps include: S1, base fabric preparation: chitosan raw materials are slurried to obtain slurry, and then spun, coagulated, stretched, and dried to obtain fibers; the fibers are then cut into short fibers, humidified, and opened to form a fiber web; the fiber web is needle-punched and shaped to obtain a base fabric; S2, loading the antibacterial and healing promoting functional solution onto the base fabric, and drying the base fabric.

9. The method for preparing the antibacterial and healing-promoting chitosan functional dressing according to claim 8, characterized in that: The slurry in S1 includes the following components in mass percentage: chitosan raw material 2%-4%, urea 0.5%-1%, propylene glycol 0.2%-0.8%, anhydrous ethanol 0.1%-0.5%, zinc acetate 0.5%-2%, and the balance is acetic acid aqueous solution; the mass fraction of the acetic acid aqueous solution is 1-5%; the loading amount of the antibacterial and healing promoting functional solution in S2 on the base fabric is 2-2.5L / m 2 The loading methods include spraying or dipping, and the drying temperature is 100℃-130℃.

10. Use of the antibacterial and healing-promoting chitosan functional dressing according to claim 6 or 7 in wound care.

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