Essence containing firming and anti-wrinkle composition and preparation method thereof

Abstract

The invention discloses essence containing a firming and anti-wrinkle composition and a preparation method of the essence, and belongs to the field of cosmetics. The firming and anti-wrinkle essence contains a firming and anti-wrinkle composition, and the composition is composed of VCHA, hydrolyzed ulva lactuca extract and ganoderma lucidum fermentation liquor. VCHA, hydrolyzed ulva lactuca extract and lucid ganoderma fermentation liquor are compounded for use and have a synergistic effect, so that excellent tightening and anti-wrinkle effects are achieved.

Description

Technical Field

[0001] The present application relates to the field of cosmetics, and in particular to an essence containing a firming and anti-wrinkle composition and a preparation method thereof. Background Art

[0002] VCHA, or ascorbic acid propylene glycol hyaluronate, is a new type of anti-wrinkle moisturizer. It not only overcomes the shortcomings of vitamin C, such as instability, poor permeability, and strong irritation, but also has the dual effects of hyaluronic acid and vitamin C, which has certain advantages. However, the current price of this raw material is 100-1000 yuan / g (depending on the purity, the price is different). Due to the high price of this raw material, it has a small audience and is difficult to promote. Therefore, how to find some plant extracts or plant fermentation extracts that can synergize with VCHA, thereby reducing the amount of VCHA and reducing costs is a problem that has attracted much attention from companies. Summary of the invention

[0003] The purpose of the present invention is to provide an essence containing a firming and anti-wrinkle composition and a preparation method thereof. The firming and anti-wrinkle composition is prepared using VCHA, hydrolyzed ulva extract, and ganoderma lucidum fermentation liquid as raw materials, has high safety, excellent firming and anti-wrinkle effects, and is cost-effective.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions: In a first aspect, the present invention provides a firming and anti-wrinkle composition, comprising the following components: VCHA, hydrolyzed ulva extract, and ganoderma lucidum fermentation liquid.

[0005] The firming and anti-wrinkle composition is composed of the following components in parts by weight: VCHA: 0.1-0.5 parts; Hydrolyzed Ulva extract: 7-10 parts; 5-7 portions of Ganoderma lucidum fermented liquid.

[0006] Furthermore, the firming and anti-wrinkle composition is composed of the following components in parts by weight: VCHA: 0.1-0.3 parts; Hydrolyzed Ulva extract: 8-10 parts; 5-6 portions of Ganoderma lucidum fermented liquid.

[0007] Most preferably, the firming and anti-wrinkle composition consists of the following components in parts by weight: VCHA: 0.1 part; Hydrolyzed Ulva Extract: 8 parts; 6 parts of Ganoderma lucidum fermented liquid.

[0008] In a second aspect, the present invention provides a firming and anti-wrinkle essence, wherein the firming and anti-wrinkle essence comprises the firming and anti-wrinkle composition of the first aspect, and specifically consists of the following components in percentage by weight: Firming and anti-wrinkle composition: 1-3%; Preservatives: 0.1-0.3%; Moisturizer: 20-25%; Make up to 100% with deionized water.

[0009] Preferably, the preservative is one or more of 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, and ethylhexylglycerin.

[0010] Preferably, the moisturizing agent is one or more of glycerin, propylene glycol, butylene glycol, sodium hyaluronate, and sodium polyglutamate.

[0011] Preferably, the firming and anti-wrinkle essence comprises the firming and anti-wrinkle composition described in the first aspect, and specifically consists of the following components in percentage by mass: Firming and anti-wrinkle composition: 1-3%; Preservatives: 0.1-0.3%; Moisturizer: 20-25%; Emollient: 0.1-15%; Active matter: 0.1-5%; Fragrance: 0.1-10%; Make up to 100% with deionized water.

[0012] Preferably, the preservative is one or more of 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, and ethylhexylglycerin.

[0013] Preferably, the moisturizing agent is one or more of glycerin, propylene glycol, butylene glycol, sodium hyaluronate, and sodium polyglutamate.

[0014] Preferably, the emollient is one or more of caprylyl polymethicone, caprylic / capric triglyceride, meadowfoam seed oil, jojoba seed oil, squalane, and isopropyl myristate.

[0015] Preferably, the active ingredients are one or more of palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, dipeptide diaminobutyryl benzylamide diacetate, and acetyl hexapeptide-8.

[0016] Preferably, the fragrance is one or more of essence, sweet orange extract, cedarwood oil, brandy leaf oil, rose water, golden chamomile water, peony water, and Rosa damascena water.

[0017] In a third aspect, the present invention provides a method for preparing a firming and anti-wrinkle essence, the specific steps of which are as follows: Add preservatives and moisturizers into deionized water, stir evenly, add hydrolyzed ulva extract and ganoderma lucidum fermentation liquid, homogenize for 2-3 hours, add VCHA and homogenize for 10-20 minutes, and sterilize to obtain a firming and anti-wrinkle essence.

[0018] Compared with the prior art, the present invention has the following beneficial effects: the present invention provides an essence containing a firming and anti-wrinkle composition and a preparation method thereof, wherein the firming and anti-wrinkle composition is compounded with VCHA, a hydrolyzed ulva extract and a ganoderma lucidum fermentation broth, and studies have found that there is a synergistic effect between VCHA and the hydrolyzed ulva extract and the ganoderma lucidum fermentation broth, specifically: on the one hand, the triterpenoid compounds and VCHA rich in the ganoderma lucidum fermentation broth both have significant antioxidant and collagen synthesis promoting effects, and the combination of the two can reduce the formation of wrinkles, and on the other hand, the hydrolyzed ulva extract can effectively promote the ganoderma lucidum fermentation broth and VCHA to penetrate into the skin, thereby better exerting their effects; in addition, the composition of the present invention is low in cost compared with VCHA of the same quality, has better effects, is cost-effective, and has market prospects. DETAILED DESCRIPTION

[0019] In order to better understand the present invention, the present invention is further described below in conjunction with specific application examples, wherein the terms used in the application examples are for describing specific implementation schemes and do not constitute limitations on the protection scope of the present invention.

[0020] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the calculation is based on mass percentage and mass fraction.

[0021] Some raw materials and their sources are as follows: VCHA: purchased from Runhui Biotechnology, CAS: 1800464-57-6, unit price: 60 yuan / g; Hydrolyzed Ulva extract: purchased from Shanghai Yuyu New Material Technology Co., Ltd., CAS: 97281-59-9, unit price: 3100 yuan / kg; Ganoderma lucidum fermentation liquid: purchased from Shanghai Lanhai Biotechnology Co., Ltd., item number: 831015, unit price: 480 yuan / kg.

[0022] The components of the firming and anti-wrinkle composition and their weight proportions are shown in Table 1.

[0023] Table 1 Components of the firming and anti-wrinkle composition and their mass ratios

[0024] The components and their mass percentages of the firming and anti-wrinkle essence are shown in Table 2.

[0025] Table 2 Components and mass percentages of the firming anti-wrinkle essence Components Application Example 1 Application Example 2 Application Example 3 Application Example 4 Application Example 5 Blank Application Example Firming anti-wrinkle composition / wt% 2 1 3 2 2 0 Firming and anti-wrinkle composition Composition 1 Composition 1 Composition 1 Composition 2 Composition 3 none Preservative / wt% 0.2 0.1 0.3 0.2 0.2 0.2 Humectant / wt% 20 25 30 20 20 20 Deionized water Make up to 100wt% Make up to 100wt% Make up to 100wt% Make up to 100wt% Make up to 100wt% Make up to 100wt% Note: The preservative is ethylhexylglycerin; the moisturizer is glycerin.

[0026] The preparation methods of the above application examples 1-5 are as follows: Add preservatives and moisturizers into deionized water, stir evenly, add hydrolyzed ulva extract and ganoderma lucidum fermentation liquid, homogenize for 2 hours, add VCHA and homogenize for 10 minutes, and sterilize to obtain a firming and anti-wrinkle essence.

[0027] The preparation method of the above blank application example is as follows: The preparation method is the same as that of Application Example 1, except that the hydrolyzed ulva extract, ganoderma lucidum fermentation liquid and VCHA are replaced with deionized water of equal mass.

[0028] Experiment 1: Collagen upregulation rate test Refer to "T / SHRH 031-2020 Cosmetic Firming and Anti-wrinkle Efficacy Test-In vitro Fibroblast Type I Collagen Content Determination", the specific steps are as follows: Samples: Compositions 1-3, compositions ①-⑦.

[0029] Cells: Human dermal fibroblasts, purchased from Jin Shaoyuan (Shanghai) Biotechnology Co., Ltd., model: JSY-2345C.

[0030] Kit: Human type I collagen (ColⅠ) ELISA kit, purchased from: Shanghai Keaibo Biotechnology Co., Ltd. Hefei Branch, model: CB10152-Hu.

[0031] In the experiment, the reagents and consumables required for cell culture were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0032] Adjust the cell concentration to 4.0 × 10 4 cell / mL, add 100 μl / well to a 96-well cell culture plate. Cover the cell plate, seal the edges with PBS, and place at 37°C and CO 2 The samples were cultured for 24 hours at a concentration of 5.0% for collagen expression testing.

[0033] The culture medium was discarded from the cultured cell plate, and the plate was washed with PBS for 1-2 times. The sample was diluted to 2wt% with DMEM and added to the cell plate. Six wells were added with the same concentration. A blank control group and a positive control group were set up. The blank control group was added with 100μl / well DMEM, and the positive control group was added with 100μl / well DMEM medium containing 100ng / mL recombinant human transforming growth factor TGF-β. After adding the sample, the plate was placed at 37℃ and CO. 2After culturing for 24 hours under the condition of 5.0% concentration, after the end of the culture, the cell culture medium was discarded, and the cells at the bottom were gently rinsed twice with PBS, discarded, and the cells at the bottom were lysed, and the lysate was collected to determine the collagen content. The cell lysis method is: add 100μl / well of cell lysis solution, place in a 2-8℃ refrigerator for lysis for 10min, and collect the lysate for use. If it cannot be determined in time, the cell fluid can be collected in a 1.5mL sterile centrifuge tube and placed in a -80℃ ultra-low temperature refrigerator for storage.

[0034] The collagen content in the cell culture fluid was tested by enzyme-linked immunosorbent assay (ELISA), and the specific test method was carried out according to the instruction manual of the human type I collagen (ColⅠ) ELISA kit. According to the standard curve of the ELISA kit, the collagen content of the sample group, blank control group and positive control group was calculated respectively.

[0035] Collagen expression up-regulation rate (%) = (TC) / C×100%.

[0036] Wherein, T is the average collagen content of the sample group, and C is the average collagen content of the blank control group.

[0037] Table 3 Collagen upregulation rate test results

[0038] Note:" "Indicates that the experimental group is significantly different from the blank group," "represent p <0.05," "represent p <0.01.

[0039] According to the test results in Table 3, the composition provided by the present invention has a significant effect of promoting collagen regeneration. By comparing the test results of composition 1 with compositions ①-⑥, it can be seen that there is a certain synergistic effect between VCHA, hydrolyzed ulva extract, and ganoderma fermentation broth in the composition. By comparing the test results of composition 1 with composition ⑦, it can be seen that the composition composed of 0.1 parts of VCHA, 8 parts of hydrolyzed ulva extract, and 6 parts of ganoderma fermentation broth in the present invention has the same improvement rate of type I collagen as that achieved by 0.5 parts of VCHA by mass in composition ⑦, but the cost is several times different. By comparing the test results of composition 3 with composition ⑦, it can be seen that under the action of hydrolyzed ulva extract and ganoderma fermentation broth, VCHA has a better improvement rate for the regeneration of type I collagen and a better anti-wrinkle effect.

[0040] Experiment 2: Anti-aging test The eye cream prepared by the present invention was subjected to an 8-week anti-aging test according to the method described in the literature (Zhu Liping et al., Clinical Efficacy Test and Analysis of Ginseng Anti-aging Mask [J], Chinese Journal of Aesthetic Medicine, 2016, 25(02): 33-36).

[0041] Ten volunteers were selected for each application case. Inclusion criteria: ① Male or female, aged 30 to 65 years old; with a certain degree of facial / eye skin sagging or fine wrinkles on the face / eyes; ② Good health, serious attitude, certain expression ability, and can truly reflect the feelings after use; ③ Voluntary participation, signing the informed consent form, strictly abide by the requirements of the research plan, use the product according to the requirements of the research plan and complete follow-up. Exclusion criteria: ① Pregnant or lactating women; ② Sensitive skin; ③ People with damaged facial skin or skin diseases that affect the observation of facial status; ④ People who have received skin treatment, beauty and other tests that may affect the results; ⑤ People who have sunburned or used hormone drugs and immunosuppressants in the past 1 month; ⑥ People who have participated in other facial clinical studies or received treatment by dermatologists currently or in the past 3 months. Termination and exclusion criteria: ① The subject requests to stop the test; ② The subject's compliance is poor; ③ The test cannot be continued due to adverse reactions or special physiological and pathological changes; ④ During the test, other skin care products that affect this experiment are used, and excessive exposure to ultraviolet rays are received.

[0042] How to use the product: After cleansing every night, volunteers apply 500-600 mg of the test sample evenly on the face. After massage and absorption, there is no need to cleanse the face or apply any other cosmetics. This procedure lasts for 8 weeks: relevant data are measured on the same part at week 0 and week 8.

[0043] Instrument measurement method: Skin elasticity tester MPA580 and VISIA facial image analysis system from Canfield, USA were used for testing. ① Facial skin elasticity measurement: Use a skin elasticity tester to measure the skin elasticity at the end of the left and right eyes and under the eyes, and calculate R 2 The average value, R 2 =Ua / Uf, Ua is the recovery value of the skin from the time when negative pressure is removed to the time when negative pressure is applied to the skin surface again during the next continuous test, and Uf is the maximum stretching of the skin when negative pressure is present.

[0044] ② Facial imaging: The VISIA facial image analysis system was used to take photos of the subjects’ faces (front, left and right sides at 45°). The wrinkle scores were extracted and the average value was taken.

[0045] Test samples: Application Examples 1-5, blank application example.

[0046] Table 4 Human efficacy test results Experimental Group <![CDATA[Δ Skin elasticity R 2 Value (week 8 - week 0) / %]]> ΔWrinkle score (week 8-week 0) / % Application Example 1 0.736 -3.4 Application Example 2 0.714 -3.1 Application Example 3 0.769 -3.5 Application Example 4 0.867 -4.3 Application Example 5 0.973 -4.9 Blank Application Example 0.029 0.2 Test results of skin elasticity: As shown in Table 4, the skin elasticity was significantly improved compared with that in week 0, and the essence prepared by the present invention showed a good effect of improving skin elasticity.

[0047] Test results of skin wrinkle scores: As shown in Table 4, compared with the volunteers in week 0, the facial wrinkle scores of the volunteers were significantly and gradually reduced, indicating that the essence prepared by the present invention has a strong effect of improving facial wrinkles and fine lines, and will not produce skin tolerance due to long-term use.

[0048] Therefore, long-term use of the essence prepared by the embodiment of the present invention by the subjects can improve skin elasticity and effectively reduce facial wrinkles, showing good anti-aging, wrinkle reduction, and skin elasticity enhancement effects.

[0049] Test 3: Patch test The present invention experiments verify the biosafety of application examples 1-5 and blank application examples.

[0050] The 2015 "Technical Specifications for Safety of Cosmetics" was used as a reference standard for the irritation evaluation of cosmetics. The test method was a skin patch test, with 10 people in each group, aged 16-65 years old, and gender was random.

[0051] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0052] Evaluation criteria: Grade 0: negative reaction; Grade 1: Suspicious reaction, only slight erythema; Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present; Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area; Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0053] Test results: Skin reactions were negative in all subjects.

[0054] Test 4: Human test on sensitive skin Test substances: Application Examples 1-5 and blank application examples.

[0055] Subject selection: healthy volunteers aged 21-44 years old, who meet the requirements of lactic acid sting test, voluntarily participate and sign the informed consent, and can complete the test as required during the test period are selected. Each group has 30 subjects.

[0056] Experimental method: Volunteers randomly divided both sides of their faces into test side and control side. After cleansing, the product was evenly applied to the facial skin of the test side according to the product usage instructions, and no product was used on the control side. After 28 days of trial, the volunteers reported local skin reactions. If there were any adverse reactions, dermatologists would evaluate the adverse reactions.

[0057] Result analysis: The classification of adverse skin reactions was determined according to the classification of adverse skin reactions in human trial tests specified in the "Technical Specifications for Safety of Cosmetics" (2015 edition). The results are shown in Table 5.

[0058] Table 5 Human trial test results

[0059] Combining the results of the human skin patch test in Experiment 3 with the sensitive skin test in this experiment, no subjects experienced adverse skin reactions in Application Examples 1-5 during the test period, indicating that Application Examples 1-5 are safe, mild and non-irritating, and suitable for people with sensitive skin.

[0060] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. A firming and anti-wrinkle composition, characterized in that: The composition consists of the following components in parts by weight: VCHA: 0.1-0.5 parts; Hydrolyzed Ulva extract: 7-10 parts; 5-7 portions of Ganoderma lucidum fermented liquid.

2. The composition according to claim 1, characterized in that The composition consists of the following components in parts by weight: VCHA: 0.1-0.3 parts; Hydrolyzed Ulva extract: 8-10 parts; 5-6 portions of Ganoderma lucidum fermented liquid.

3. The composition according to claim 1, characterized in that The composition consists of the following components in parts by weight: VCHA: 0.1 part; Hydrolyzed Ulva Extract: 8 parts; 6 parts of Ganoderma lucidum fermented liquid.

4. A firming and anti-wrinkle essence, characterized in that: The essence comprises the firming and anti-wrinkle composition according to any one of claims 1 to 3.

5. The essence according to claim 4, characterized in that: The essence is composed of the following components in percentage by weight: Firming and anti-wrinkle composition: 1-3%; Preservatives: 0.1-0.3%; Moisturizer: 20-25%; Make up to 100% with deionized water.

6. The essence according to claim 5, characterized in that: The preservative is one or more of 1,2-pentanediol, 1,2-hexanediol, caprylyl glycol, and ethylhexylglycerin.

7. The essence according to claim 5, characterized in that: The moisturizing agent is one or more of glycerin, propylene glycol, butylene glycol, sodium hyaluronate, and sodium polyglutamate.

8. A method for preparing the essence according to any one of claims 5 to 7, characterized in that: The following steps are involved: Add preservatives and moisturizers into deionized water, stir evenly, add hydrolyzed ulva extract and ganoderma lucidum fermentation liquid, homogenize for 2-3 hours, add VCHA and homogenize for 10-20 minutes, and sterilize to obtain a firming and anti-wrinkle essence.

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