Sample sampling device for preparing stem cells

Abstract

The invention discloses a sampling device for preparing stem cells, and relates to the technical field of pollution prevention, the sampling device comprises a rack, a pretreatment mechanism and a sampling mechanism, the pretreatment mechanism comprises an electric telescopic rod, a pressing piece is fixedly mounted at the telescopic end of the electric telescopic rod, and a liquid storage pipe is fixedly mounted at the top end of the pressing piece; the sampling mechanism comprises a sampler and a conical guide sleeve, a hose is installed in the middle of the bottom of the sampler, and the end, away from the sampler, of the hose is communicated with a material taking round head. A sealing ring is fixedly installed at a feeding port in the top of the material taking round head, and a jacking assembly is installed at the position, close to the material taking round head, of the bottom of the inner cavity of the shell. According to the sample sampling device for preparing the stem cells, the anti-pollution effect is achieved, contact between the stem cells and air is reduced, pollution is not likely to be caused, and sampling is safe and reliable.

Description

Technical Field

[0001] The invention relates to the technical field of sampling equipment, in particular to a sample sampling device for preparing stem cells. Background Art

[0002] Stem cells are a type of cells with self-replication and multidirectional differentiation potential. Under certain conditions, they can differentiate into a variety of functional cells and tissues, and have biological functions such as immune regulation and regeneration and repair. Stem cell secretions are a variety of active substances secreted by stem cells after special cultivation, including proteins, cytokines, peptides, etc., which are effective in anti-aging, beauty, cell repair, etc.; stem cell sampling plays a great role in the cultivation and research of stem cells. When processing blood, stem cells need to be separated and stem cell samples need to be sampled, so stem cell sampling equipment is needed.

[0003] Currently, during the existing sampling of stem cells, the stem cells are exposed to the outside, so that the stem cells are in direct contact with the air, which can easily cause contamination of the stem cells, thereby reducing the activity of the stem cells and affecting the sampling of the stem cells. Summary of the invention

[0004] To achieve the above objectives, the present invention is implemented through the following technical solutions: A sample sampling device for preparing stem cells, comprising: A frame, the frame having a supporting frame body, a shell is installed on the top of the frame, and a feeding mechanism is installed in the middle of the top of the shell; A pretreatment mechanism, which is used to stratify blood, and is installed in the middle of the top of the inner cavity of the housing; Wherein, the pretreatment mechanism comprises an electric telescopic rod, which is installed at the side of the top of the inner cavity of the shell, a pressing piece is fixedly installed at the telescopic end of the electric telescopic rod, a night storage tube is fixedly installed at the top of the pressing piece, an opening and closing component is installed at the bottom of the night storage tube, an elastic telescopic sleeve is fixedly installed between the outer cylindrical surface of the night storage tube and the top of the inner cavity of the shell, and a magnetic blocking ball is fixedly installed at the inclined surface of the inner cavity of the night storage tube and near the top. The telescopic end of the electric telescopic rod is contracted to drive the night storage tube to move upward, and the elastic telescopic sleeve is compressed. Combined with the top of the night storage tube being inside the elastic telescopic sleeve, it can be isolated from the outside air and is not easily polluted by the outside air, thereby helping the survival of stem cells; A sampling mechanism, which is used to collect stem cells and is installed at the bottom of the housing and close to the frame; Wherein, the sampling mechanism includes a sampler and a conical guide sleeve, the sampler is installed at the side of the outer side of the shell, the sampler is installed at a position close to the frame, the conical guide sleeve is fixedly installed in the middle of the bottom of the shell, a hose is installed in the middle of the bottom of the sampler, the hose passes through the center of the conical guide sleeve and extends to the inside of the shell, the end of the hose away from the sampler is connected with a material taking round head, a sealing ring is fixedly installed at the feed inlet on the top of the material taking round head, a jacking assembly is installed at the bottom of the inner cavity of the shell and close to the material taking round head, a pushing force is applied to the material taking round head by the jacking assembly, so that the material taking round head moves upward, and room is left in combination with the hose, so that the material taking round head moves upward smoothly, and it is not easy to get stuck in the structure, and the upward moving material taking round head coincides with the bottom end of the night storage tube; At the same time, the staff turns on the sampler and uses the suction generated by the sampler and the conveying of the hose to allow the round head to suck out the stem cells so that samples can be collected.

[0005] Preferably, there are two electric telescopic rods, and the two electric telescopic rods are symmetrically installed along the night storage tube. The night storage tube passes through the center of the elastic telescopic sleeve, so as to isolate the top of the night storage tube through the elastic telescopic sleeve.

[0006] After the mixed liquid material enters the storage tube, the material can be left to stand to allow the mixed material to be stratified, the stem cells to be separated, and the material to be pre-treated, thereby facilitating the sampling of the stem cells.

[0007] Preferably, the opening and closing assembly includes a right-angle elastic sheet and an annular positioning sleeve, the right-angle elastic sheet is fixedly mounted on the inner wall of the night storage tube and close to the bottom, the annular positioning sleeve is fixedly mounted on the bottom end of the night storage tube, and a ball is fixedly mounted on one end of the right-angle elastic sheet away from the inner wall of the night storage tube.

[0008] Preferably, the right-angle elastic sheets are evenly distributed between the spherical surface outside the sphere and the inner wall of the night storage tube, and the annular positioning sleeve is installed directly below the night storage tube.

[0009] Preferably, the sealing ring is made of rubber material, which is convenient for sealing the edge of the feed inlet at the top of the feeding round head. The lifting components are in two groups, and the two groups of lifting components are symmetrically installed along the feeding round head.

[0010] Preferably, the jacking assembly includes a U-shaped bracket and an arched pushing rod, the opening of the U-shaped bracket is downward, and the bottom end of the U-shaped bracket is fixedly installed with the bottom of the inner cavity of the shell, an arc-shaped hole is opened in the middle of the outer side of the arched pushing rod, and the pin shaft at the top of the U-shaped bracket passes through the middle of the arc-shaped hole, the connecting end of the outer side of the arched pushing rod is hinged to the bottom of the spherical surface of the material-retrieving round head, and a return spring is fixedly installed between the middle of the bottom of the arched pushing rod and the bottom of the inner cavity of the shell, and when the output end of the electric telescopic rod is extended, the pressing piece is driven to move downward, and the storage The night tube moves downward together, and the pressing piece applies pressing force to the end of the arched push rod away from the material taking round head, and under the support of the U-shaped bracket and the sliding installation through the arc hole, the material taking round head is lifted up, and the return spring is stretched, and the upward moving material taking round head is embedded in the annular positioning sleeve, and the material taking round head applies an upward pushing force to the ball, so that the ball moves upward, and the right-angle elastic sheet is compressed, and the discharge hole at the bottom of the night storage tube is opened, and under the sealing of the sealing ring, it is convenient to take the stem cells at the bottom of the night storage tube through the material taking round head.

[0011] Preferably, there are two arched push rods, and the two arched push rods are symmetrically installed along the material picking round head, and the reset spring is installed directly below the arched push rod to facilitate applying elastic tension to the arched push rod, thereby helping the arched push rod to reset.

[0012] Preferably, the feeding mechanism includes a square box and a discharge port, the edge of the bottom of the square box is fixedly installed with the top of the shell by screws, the discharge port is opened at the bottom of the square box, and the position of the discharge port corresponds to the position of the magnetic sealing ball, a reagent feeder is installed at the side of the top of the square box, a right-angle tube is installed on the top of the square box and on the side away from the reagent feeder, a servo motor is installed in the middle of the top of the square box, a rotating roller is rotatably installed in the middle of the inner cavity of the square box, the top of the rotating roller is fixedly installed with the output end of the servo motor through a coupling, a mixing blade is fixedly installed in the middle of the outer cylindrical surface of the rotating roller, and a flipping assembly is installed on the outer cylindrical surface of the rotating roller near the bottom. When the storage tube moves upward, the magnetic sealing ball can be driven to move upward together, and the position of the magnetic sealing ball corresponds to the position of the discharge port, so that the magnetic sealing ball can be embedded in the inside of the discharge port, thereby sealing the discharge port, and it is not easy to leak material at will.

[0013] By extending the output end of the electric telescopic rod, the night storage tube can be driven to move downward, so that the magnetic sealing ball can be driven to move downward, thereby separating the magnetic sealing ball from the discharge port, so that the discharge port is opened, and the mixed liquid material flows downward from the discharge port and is promptly connected to the night storage tube for subsequent processing.

[0014] By rotating the output end of the servo motor, the rotating roller can be driven to rotate, so that the mixing blades are driven to rotate together, and the rotating mixing blades can be used to mix the blood and reagents in the square box.

[0015] Preferably, the flipping assembly includes a supporting round rod, which is fixedly mounted on the outer circumferential surface of the rotating roller and close to the bottom position, and a flipping plate is rotatably mounted on the outer circumferential surface of the supporting round rod and close to the rotating roller, and a torque spring is fixedly mounted between the end of the supporting round rod away from the rotating roller and the side of the outer side of the flipping plate, and a magnetic ball is fixedly mounted on the outer side of the flipping plate and close to the bottom position, and the magnetic ball and the magnetic sealing ball have the same magnetic poles. When the rotating roller rotates, it will drive the flipping assembly to rotate as a whole, and utilize the magnetic ball and the magnetic sealing ball to approach each other so that the two generate repulsive magnetic force, and under the support of the supporting round rod, the flipping plate is subjected to the reverse magnetic thrust to rotate counterclockwise to adjust the angle, and the torque spring is subjected to the torque force to be elastically compressed; As the flipping plate drives the magnetic ball to continue to rotate, the magnetic ball moves away from the magnetic blocking ball, the reverse magnetic thrust disappears, and under the elastic force of the torque spring, the flipping plate rotates in the opposite direction. In this way, the blood and reagents in the square box can be flipped by the reciprocating swing of the flipping plate, so that the materials are fully integrated together, and the structures are connected together by utilizing the interaction between the structures.

[0016] Preferably, the flipping plate is arc-shaped, there are two flipping plates, and the two flipping plates are symmetrically installed along the axis of the rotating roller, and the supporting round rod passes through the center of the torque spring.

[0017] The present invention provides a sample sampling device for preparing stem cells. It has the following beneficial effects: 1. The sample sampling device for preparing stem cells applies a pushing force to the material collecting round head through a lifting component, so that the material collecting round head moves upward, and a room is left in combination with a hose, so that the material collecting round head moves upward smoothly, and it is not easy for the structure to get stuck, and the upward moving material collecting round head coincides with the bottom end of the night storage tube. At the same time, the staff turns on the sampler to work, uses the suction generated by the sampler, and under the transportation of the hose, the material collecting round head sucks out the stem cells, and then the sample can be collected.

[0018] 2. The sample sampling device for preparing stem cells utilizes the telescopic end of the electric telescopic rod to contract, so that the storage tube can be driven to move upward, and the elastic telescopic sleeve is compressed. In combination with the top of the storage tube being inside the elastic telescopic sleeve, it can be isolated from the outside air and is not easily contaminated by the outside air, thereby helping the survival of stem cells.

[0019] 3. The sample sampling device for preparing stem cells can drive the magnetic sealing ball to move upward when the storage tube moves upward, and by making the position of the magnetic sealing ball correspond to the position of the discharge port, the magnetic sealing ball can be embedded in the inside of the discharge port, thereby sealing the discharge port, and it is not easy to leak material randomly.

[0020] Fourth, when the blood and reagent are mixed, the sample sampling device for preparing stem cells can extend the output end of the electric telescopic rod again to drive the storage tube to move downward, so that the magnetic sealing ball can be driven to move downward, thereby separating the magnetic sealing ball from the discharge port, opening the discharge port, and allowing the mixed liquid material to flow downward from the discharge port and be promptly connected to the storage tube for subsequent processing.

[0021] 5. The sample sampling device for preparing stem cells can allow the mixed liquid material to stand still after it enters the storage tube, so that the mixed material can be layered, the stem cells can be separated, and the material can be pre-treated, which is helpful for sampling the stem cells.

[0022] 6. The sample sampling device for preparing stem cells uses a pressing piece to apply a pressing force to the end of the arched push rod away from the material collection round head, so that the material collection round head is pushed up, and the upwardly moving material collection round head is embedded in the annular positioning sleeve, and an upward pushing force is applied to the ball through the material collection round head, so that the ball moves upward, and the right-angle elastic sheet is compressed, and the discharge hole at the bottom of the storage tube is opened. Under the sealing of the sealing ring, it is convenient to use the material collection round head to collect the stem cells at the bottom of the storage tube.

[0023] 7. The sample sampling device for preparing stem cells can rotate the output end of the servo motor to drive the rotating roller to rotate, so that the mixing blades are driven to rotate together, and the rotating mixing blades can be used to mix the blood and reagents in the square box.

[0024] 8. The sample sampling device for preparing stem cells utilizes the repulsive magnetic force generated by the proximity of a magnetic ball and a magnetic sealing ball, so that a flipping plate is subjected to a reverse magnetic thrust to rotate counterclockwise to adjust the angle. The flipping plate drives the magnetic ball to rotate continuously, so that the magnetic ball moves away from the magnetic sealing ball, the reverse magnetic thrust disappears, and the flipping plate rotates in the opposite direction under the elastic force of the torque spring. In this way, the blood and reagents in the square box can be flipped by the reciprocating swing of the flipping plate, so that the materials are fully integrated together. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the overall structure of the sample sampling device for preparing stem cells according to the present invention; Figure 2 This is a schematic diagram of the cross-sectional structure of a sample sampling device for preparing stem cells according to the present invention; Figure 3 It is a schematic diagram of the connection structure between the pretreatment mechanism and the housing of the present invention; Figure 4 This is a schematic diagram of the overall structure of the opening and closing assembly of the present invention; Figure 5 It is a schematic diagram of the connection structure between the sampling mechanism and the housing of the present invention; Figure 6 It is a schematic diagram of the overall structure of the jacking assembly of the present invention; Figure 7 It is a schematic diagram of the connection structure between the feeding mechanism and the housing of the present invention; Figure 8 It is a schematic diagram of the overall structure of the flip assembly of the present invention.

[0026] In the figure: 1, frame; 2, shell; 3, pretreatment mechanism; 4, sampling mechanism; 5, feeding mechanism; 31, electric telescopic rod; 32, pressing piece; 33, night storage tube; 34, opening and closing assembly; 35, elastic telescopic sleeve; 36, magnetic blocking ball; 341, right-angle elastic sheet; 342, annular positioning sleeve; 343, round ball; 41, sampler; 42, conical guide sleeve; 43, hose; 44, round head for taking material; 45 , sealing ring; 46, lifting assembly; 461, U-shaped bracket; 462, arched push rod; 463, arc hole; 464, reset spring; 51, square box; 52, reagent feeder; 53, right-angle tube; 54, servo motor; 55, rotating roller; 56, mixing blade; 57, flipping assembly; 58, discharge port; 571, supporting round rod; 572, flipping plate; 573, torque spring; 574, magnetic ball. DETAILED DESCRIPTION

[0027] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present invention are provided for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

[0028] The first embodiment, as Figure 1-Figure 4 As shown, the present invention provides a technical solution: a sample sampling device for preparing stem cells, comprising: A frame 1 having a supporting frame body, a housing 2 is installed on the top of the frame 1, and a feeding mechanism 5 is installed in the middle of the top of the housing 2; A pretreatment mechanism 3, which is used to stratify blood and is installed in the middle of the top of the inner cavity of the housing 2; The pretreatment mechanism 3 comprises an electric telescopic rod 31, which is mounted on the side of the top of the inner cavity of the shell 2, a pressing piece 32 is fixedly mounted on the telescopic end of the electric telescopic rod 31, a night storage tube 33 is fixedly mounted on the top of the pressing piece 32, an opening and closing assembly 34 is mounted on the bottom of the night storage tube 33, an elastic telescopic sleeve 35 is fixedly mounted between the outer cylindrical surface of the night storage tube 33 and the top of the inner cavity of the shell 2, and a magnetic blocking ball 36 is fixedly mounted on the inclined surface of the inner cavity of the night storage tube 33 and near the top. When the staff turns on the electric telescopic rod 31 to work and uses the telescopic end of the electric telescopic rod 31 to contract, the night storage tube 33 can be driven to move upward, and the elastic telescopic sleeve 35 is compressed, and combined with the top of the night storage tube 33 being inside the elastic telescopic sleeve 35, it can be isolated from the outside air and is not easily polluted by the outside air, thereby helping the survival of stem cells; There are two electric telescopic rods 31, and the two electric telescopic rods 31 are symmetrically installed along the night storage tube 33. The night storage tube 33 passes through the center of the elastic telescopic sleeve 35, so that the top of the night storage tube 33 can be isolated by the elastic telescopic sleeve 35.

[0029] After the mixed liquid material enters the storage tube 33, the material can be left to stand to allow the mixed material to be stratified, the stem cells to be separated, and the material to be pre-treated, thereby facilitating the sampling of the stem cells.

[0030] The opening and closing assembly 34 includes a right-angle elastic piece 341 and an annular positioning sleeve 342. The right-angle elastic piece 341 is fixedly installed on the inner wall of the night storage tube 33 and close to the bottom. The annular positioning sleeve 342 is fixedly installed on the bottom end of the night storage tube 33. A ball 343 is fixedly installed on one end of the right-angle elastic piece 341 away from the inner wall of the night storage tube 33.

[0031] The right-angle elastic pieces 341 are evenly distributed between the spherical surface outside the sphere 343 and the inner wall of the night storage tube 33 , and the annular positioning sleeve 342 is installed just below the night storage tube 33 .

[0032] The second embodiment, as Figure 1-Figure 6 As shown, based on the first embodiment: A sampling mechanism 4, which is used to collect stem cells, and is installed at the bottom of the housing 2 and close to the frame 1; The sampling mechanism 4 includes a sampler 41 and a conical guide sleeve 42. The sampler 41 is installed at the side of the outer side of the shell 2. The sampler 41 is installed at a position close to the frame 1. The conical guide sleeve 42 is fixedly installed in the middle of the bottom of the shell 2. A hose 43 is installed in the middle of the bottom of the sampler 41. The hose 43 passes through the center of the conical guide sleeve 42 and extends to the inside of the shell 2. The end of the hose 43 away from the sampler 41 is connected with a material taking round head 44. A sealing ring 45 is fixedly installed at the feed inlet on the top of the material taking round head 44. A lifting component 46 is installed at the bottom of the inner cavity of the shell 2 and close to the material taking round head 44. A driving force is applied to the material taking round head 44 through the lifting component 46, so that the material taking round head 44 moves upward, and a leeway is left in combination with the hose 43, so that the material taking round head 44 moves upward smoothly, and it is not easy to get stuck in the structure, and the upward moving material taking round head 44 coincides with the bottom end of the night storage tube 33. At the same time, the staff turns on the sampler 41 to work, and uses the suction force generated by the sampler 41 and the conveyance of the hose 43 to make the material collection round head 44 suck out the stem cells, so that the sample can be collected.

[0033] The sealing ring 45 is made of rubber, which is convenient for sealing the edge of the feeding port at the top of the feeding round head 44 . There are two groups of lifting components 46 , and the two groups of lifting components 46 are symmetrically installed along the feeding round head 44 .

[0034] The lifting assembly 46 includes a U-shaped bracket 461 and an arched push rod 462. The opening of the U-shaped bracket 461 is downward, and the bottom end of the U-shaped bracket 461 is fixedly installed with the bottom of the inner cavity of the shell 2. An arc hole 463 is opened in the middle of the outer side of the arched push rod 462. The pin shaft at the top of the U-shaped bracket 461 passes through the middle of the arc hole 463. The connecting end of the outer side of the arched push rod 462 is hinged to the bottom of the spherical surface of the material-taking round head 44. A return spring 464 is fixedly installed between the middle of the bottom of the arched push rod 462 and the bottom of the inner cavity of the shell 2. When the output end of the electric telescopic rod 31 is extended, the pressing piece 32 is driven to move downward, and the storage tube 33 moves upward together. The pressing piece 32 is used to apply a pressing force to the end of the arched push rod 462 away from the material collection round head 44, and under the support of the U-shaped bracket 461 and the sliding installation of the arc hole 463, the material collection round head 44 is lifted up, and the return spring 464 is stretched, and the upward moving material collection round head 44 is embedded in the annular positioning sleeve 342, and the material collection round head 44 applies an upward pushing force to the ball 343, so that the ball 343 moves upward, and the right-angle elastic sheet 341 is compressed, and the discharge hole at the bottom of the night storage tube 33 is opened, and under the sealing of the sealing ring 45, it is convenient to take the stem cells at the bottom of the night storage tube 33 through the material collection round head 44.

[0035] There are two arched push rods 462 , and the two arched push rods 462 are symmetrically installed along the material taking round head 44 . The reset spring 464 is installed directly below the arched push rod 462 to apply elastic tension to the arched push rod 462 , thereby helping the arched push rod 462 to reset.

[0036] The third embodiment, as Figure 1-Figure 8 As shown, based on the first and second embodiments: The feeding mechanism 5 includes a square box 51 and a discharge port 58. The edge of the bottom of the square box 51 is fixed to the top of the shell 2 by screws. The discharge port 58 is opened at the bottom of the square box 51. The position of the discharge port 58 corresponds to the position of the magnetic blocking ball 36. A reagent feeder 52 is installed on the side of the top of the square box 51. A right-angle tube 53 is installed on the top of the square box 51 and away from the side of the reagent feeder 52. A servo motor 54 is installed in the middle of the top of the square box 51. A rotating roller 55 is rotatably installed in the middle of the inner cavity of the square box 51. The top of the moving roller 55 is fixedly installed with the output end of the servo motor 54 through a coupling, a mixing blade 56 is fixedly installed in the middle of the outer cylindrical surface of the rotating roller 55, and a flipping assembly 57 is installed on the outer cylindrical surface of the rotating roller 55 near the bottom. When the night storage tube 33 moves upward, it can drive the magnetic blocking ball 36 to move upward together, and by making the position of the magnetic blocking ball 36 correspond to the position of the discharge port 58, the magnetic blocking ball 36 can be embedded in the inside of the discharge port 58, thereby blocking the discharge port 58, and it is not easy to leak materials at will.

[0037] When the blood and reagent are mixed, the electric telescopic rod 31 can be turned on again to work. By extending the output end of the electric telescopic rod 31, the night storage tube 33 can be driven to move downward, and the magnetic blocking ball 36 can be driven to move downward, thereby separating the magnetic blocking ball 36 from the discharge port 58, so that the discharge port 58 is opened, and the mixed liquid material flows downward from the discharge port 58 and is promptly connected to the night storage tube 33.

[0038] The staff injects blood into the square box 51 through the right-angle tube 53, injects reagent into the square box 51 through the reagent feeder 52, and turns on the servo motor 54 to work. By rotating the output end of the servo motor 54, the rotating roller 55 can be driven to rotate, so that the mixing blade 56 is driven to rotate together, and the rotating mixing blade 56 can be used to mix the blood and reagent in the square box 51.

[0039] The flipping assembly 57 includes a supporting round rod 571, which is fixedly mounted on the outer circumferential surface of the rotating roller 55 and close to the bottom position. A flipping plate 572 is rotatably mounted on the outer circumferential surface of the supporting round rod 571 and close to the rotating roller 55. A torque spring 573 is fixedly mounted between the end of the supporting round rod 571 away from the rotating roller 55 and the side of the outer side of the flipping plate 572. A magnetic ball 574 is fixedly mounted on the outer side of the flipping plate 572 and close to the bottom position. The magnetic ball 574 and the magnetic blocking ball 36 have the same magnetic poles. When the rotating roller 55 rotates, it will drive the flipping assembly 57 to rotate as a whole, and when the magnetic ball 574 and the magnetic blocking ball 36 are close to each other, the two will generate a repulsive magnetic force, and under the support of the supporting round rod 571, the flipping plate 572 is subjected to the reverse magnetic thrust to rotate counterclockwise to adjust the angle, and the torque spring 573 is subjected to the torque force to be elastically compressed; As the flipping plate 572 drives the magnetic ball 574 to continue to rotate, the magnetic ball 574 moves away from the magnetic blocking ball 36, the reverse magnetic thrust disappears, and under the elastic force of the torque spring 573, the flipping plate 572 rotates in the opposite direction. In this way, the blood and reagents in the square box 51 can be flipped by the reciprocating swing of the flipping plate 572, so that the materials can be fully integrated together.

[0040] The material turning plate 572 is arc-shaped. There are two material turning plates 572 , and the two material turning plates 572 are installed symmetrically along the axis of the rotating roller 55 . The supporting round rod 571 passes through the center of the torque spring 573 .

[0041] When in use, first, when the ball 343 is in the initial state, the ball 343 is subjected to the elastic pressing force of the right-angle elastic sheet 341, and the liquid outlet hole at the bottom end of the night storage tube 33 is blocked; The staff turns on the electric telescopic rod 31 to work, and uses the telescopic end of the electric telescopic rod 31 to contract, so that the night storage tube 33 can be driven to move upward, and the elastic telescopic sleeve 35 is compressed, and the position of the magnetic blocking ball 36 corresponds to the position of the discharge port 58, so that the magnetic blocking ball 36 can be embedded in the discharge port 58, thereby blocking the discharge port 58; In addition, the top of the night storage tube 33 is located inside the elastic telescopic sleeve 35, so that it can be isolated from the outside air and is not easily polluted by the outside air, thereby facilitating the survival of stem cells; At this time, the staff injects blood into the square box 51 through the right-angle tube 53, injects reagent into the square box 51 through the reagent feeder 52, and turns on the servo motor 54 to work. The output end of the servo motor 54 rotates to drive the rotating roller 55 to rotate, so that the mixing blade 56 is driven to rotate together, and the rotating mixing blade 56 can be used to mix the blood and reagent in the square box 51; At the same time, when the rotating roller 55 rotates, it will drive the flipping assembly 57 to rotate as a whole, and when the magnetic ball 574 and the magnetic blocking ball 36 are close to each other, the two will generate a repulsive magnetic force, and under the support of the supporting round rod 571, the flipping plate 572 is subjected to the reverse magnetic thrust to rotate counterclockwise to adjust the angle, and the torque spring 573 is subjected to the torque force to be elastically compressed; As the material turning plate 572 drives the magnetic ball 574 to continue to rotate, the magnetic ball 574 moves away from the magnetic blocking ball 36, the reverse magnetic thrust disappears, and under the elastic force of the torque spring 573, the material turning plate 572 rotates in the opposite direction, so that the blood and reagents in the square box 51 can be turned over by the reciprocating swing of the material turning plate 572; When the blood and reagent are mixed, the electric telescopic rod 31 can be turned on again to work. The output end of the electric telescopic rod 31 is extended to drive the night storage tube 33 to move downward, so that the magnetic blocking ball 36 can be driven to move downward, thereby separating the magnetic blocking ball 36 from the discharge port 58, so that the discharge port 58 is opened, and the mixed liquid material flows downward from the discharge port 58 and is promptly received by the night storage tube 33. And when the mixed liquid material enters the storage tube 33, the material can be left to stand, so that the mixed material can be layered, the stem cells can be separated, and the material can be pre-treated; When the output end of the electric telescopic rod 31 is extended, the pressing member 32 is driven to move downward, and the night storage tube 33 moves downward together, and the pressing member 32 applies a pressing force to the end of the arched push rod 462 away from the material taking round head 44, and under the support of the U-shaped bracket 461 and the sliding installation through the arc hole 463, the material taking round head 44 is lifted up, and the return spring 464 is stretched, and the upward moving material taking round head 44 is embedded in the annular positioning sleeve 342, and the material taking round head 44 applies an upward pushing force to the ball 343, so that the ball 343 moves upward, and the right-angle elastic sheet 341 is compressed, and the material discharge hole at the bottom of the night storage tube 33 is opened; At this time, the lifting component 46 applies a pushing force to the material taking round head 44, so that the material taking round head 44 moves upward, and the hose 43 leaves room, so that the material taking round head 44 moves upward smoothly, and the structure is not easily stuck, and the upwardly moving material taking round head 44 coincides with the bottom end of the night storage tube 33; At the same time, the staff turns on the sampler 41 to work, and uses the suction force generated by the sampler 41 and the conveyance of the hose 43 to make the material collection round head 44 suck out the stem cells, so that the sample can be collected.

[0042] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention are implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. A sample sampling device for preparing stem cells, characterized in that: include: A frame (1), the frame (1) having a frame body with a supporting function, a shell (2) being installed on the top of the frame (1), and a loading mechanism (5) being installed in the middle of the top of the shell (2); A pretreatment mechanism (3), the pretreatment mechanism (3) is used to stratify blood, and the pretreatment mechanism (3) is installed in the middle of the top of the inner cavity of the housing (2); The pretreatment mechanism (3) comprises an electric telescopic rod (31), the electric telescopic rod (31) being mounted on the side of the top of the inner cavity of the housing (2), a pressing piece (32) being fixedly mounted on the telescopic end of the electric telescopic rod (31), a night storage tube (33) being fixedly mounted on the top of the pressing piece (32), an opening and closing assembly (34) being mounted on the bottom of the night storage tube (33), an elastic telescopic sleeve (35) being fixedly mounted between the outer cylindrical surface of the night storage tube (33) and the top of the inner cavity of the housing (2), and a magnetic blocking ball (36) being fixedly mounted on the inclined surface of the inner cavity of the night storage tube (33) and close to the top; A sampling mechanism (4), the sampling mechanism (4) is used to collect stem cells, and the sampling mechanism (4) is installed at the bottom of the housing (2) and close to the frame (1); The sampling mechanism (4) comprises a sampler (41) and a conical guide sleeve (42), wherein the sampler (41) is mounted on the side of the outer side of the housing (2), the sampler (41) is mounted near the frame (1), the conical guide sleeve (42) is fixedly mounted in the middle of the bottom of the housing (2), a hose (43) is mounted in the middle of the bottom of the sampler (41), the hose (43) passes through the center of the conical guide sleeve (42) and extends to the inside of the housing (2), one end of the hose (43) away from the sampler (41) is connected to a material taking round head (44), a sealing ring (45) is fixedly mounted at the material inlet at the top of the material taking round head (44), and a lifting assembly (46) is mounted at the bottom of the inner cavity of the housing (2) and near the material taking round head (44).

2. A sample sampling device for preparing stem cells according to claim 1, characterized in that: There are two electric telescopic rods (31), and the two electric telescopic rods (31) are symmetrically installed along the night storage tube (33), and the night storage tube (33) passes through the center of the elastic telescopic sleeve (35).

3. A sample sampling device for preparing stem cells according to claim 1, characterized in that: The opening and closing assembly (34) comprises a right-angle elastic piece (341) and an annular positioning sleeve (342), wherein the right-angle elastic piece (341) is fixedly mounted on the inner wall of the night storage tube (33) and close to the bottom, and the annular positioning sleeve (342) is fixedly mounted on the bottom end of the night storage tube (33), and a round ball (343) is fixedly mounted on one end of the right-angle elastic piece (341) away from the inner wall of the night storage tube (33).

4. A sample collection device for preparing stem cells according to claim 3, characterized in that: The right-angle elastic pieces (341) are evenly distributed between the spherical surface outside the sphere (343) and the inner wall of the night storage tube (33), and the annular positioning sleeve (342) is installed directly below the night storage tube (33).

5. The sample sampling device for preparing stem cells according to claim 1, characterized in that: The sealing ring (45) is made of rubber. The lifting components (46) are in two groups, and the two groups of lifting components (46) are symmetrically installed along the material taking round head (44).

6. A sample sampling device for preparing stem cells according to claim 1, characterized in that: The lifting assembly (46) comprises a U-shaped bracket (461) and an arched push rod (462), the opening of the U-shaped bracket (461) is downward, and the bottom end of the U-shaped bracket (461) is fixedly mounted to the bottom of the inner cavity of the shell (2), an arc-shaped hole (463) is provided in the middle of the outer side of the arched push rod (462), a pin shaft at the top of the U-shaped bracket (461) passes through the middle of the arc-shaped hole (463), the outer connecting end of the arched push rod (462) is hinged to the bottom of the spherical surface of the material taking round head (44), and a return spring (464) is fixedly mounted between the middle of the bottom of the arched push rod (462) and the bottom of the inner cavity of the shell (2).

7. A sample sampling device for preparing stem cells according to claim 6, characterized in that: There are two arched push rods (462), and the two arched push rods (462) are symmetrically installed along the material taking round head (44), and the return spring (464) is installed directly below the arched push rods (462).

8. The sample collection device for preparing stem cells according to claim 1, characterized in that: The feeding mechanism (5) comprises a square box (51) and a discharge port (58). The edge of the bottom of the square box (51) is fixed to the top of the housing (2) by means of screws. The discharge port (58) is opened at the bottom of the square box (51). The position of the discharge port (58) corresponds to the position of the magnetic blocking ball (36). A reagent feeder (52) is installed at the side of the top of the square box (51). The top of the square box (51) is away from the reagent feeder (52). A right-angle tube (53) is installed on one side, a servo motor (54) is installed in the middle of the top of the square box (51), a rotating roller (55) is rotatably installed in the middle of the inner cavity of the square box (51), the top end of the rotating roller (55) is fixedly installed with the output end of the servo motor (54) through a coupling, a mixing blade (56) is fixedly installed in the middle of the outer cylindrical surface of the rotating roller (55), and a flipping assembly (57) is installed on the outer cylindrical surface of the rotating roller (55) near the bottom.

9. A sample collection device for preparing stem cells according to claim 8, characterized in that: The flipping assembly (57) comprises a supporting round rod (571), the supporting round rod (571) being fixedly mounted on the outer circumferential surface of the rotating roller (55) and close to the bottom; a flipping plate (572) is rotatably mounted on the outer circumferential surface of the supporting round rod (571) and close to the rotating roller (55); a torque spring (573) is fixedly mounted between an end of the supporting round rod (571) away from the rotating roller (55) and an outer side of the flipping plate (572); a magnetic ball (574) is fixedly mounted on the outer side of the flipping plate (572) and close to the bottom; the magnetic ball (574) and the magnetic blocking ball (36) have the same magnetic poles.

10. A sample sampling device for preparing stem cells according to claim 9, characterized in that: The flipping plate (572) is arc-shaped, there are two flipping plates (572), and the two flipping plates (572) are symmetrically installed along the axis of the rotating roller (55), and the supporting round rod (571) passes through the center of the torque spring (573).

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