Molecular detection system and device with sample heating and quantitative sample adding functions

Abstract

The invention relates to the technical field of nucleic acid detection, in particular to a molecular detection system and device with sample heating and quantitative sample adding functions. The invention provides a molecule detection device with sample heating and quantitative sample adding functions, the molecule detection device comprises a base, an optical acquisition system and at least one detection module, the detection module comprises a detection cavity, a driving system, a fluid control system and an auxiliary heating system; the detection module is arranged on the base, a detection cavity is formed in the detection module, and an insertion opening allowing a reagent card to be inserted is formed in the top of the detection cavity; the driving system, the fluid control system and the auxiliary heating system are sequentially arranged in the detection cavity, and the socket is located between the fluid control system and the auxiliary heating system. The problems that in the prior art, operation is tedious, time is long, and errors are large are effectively solved, sample PCR detection can be rapidly completed, only one-step sample adding is needed, and no pipetting step or extra manual operation is needed.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to a molecular detection system and device with sample heating and quantitative sample addition functions. Background Art

[0002] The sample release agent is used to quickly release the nucleic acid (DNA / RNA) in the sample to be tested, and is directly used for subsequent conventional PCR amplification detection. The use of sample release agents reduces the nucleic acid extraction process, greatly simplifies the nucleic acid detection process, shortens the detection time, and improves the detection efficiency. The existing technology mainly includes the following PCR detection methods:

[0003] The first method: conventional PCR instrument + release agent + manual pipette quantitative addition method: collect samples and add them to the sample release agent, manually turn them upside down for 1 second, let them stand at room temperature for 5 minutes, the samples are not heated, the operator uses the pipette to manually add the release agent containing the sample to the reaction tube, and then test it on the machine. This method has the following disadvantages: it takes a long time, cannot achieve rapid detection, does not go through ultrasound or heating steps, takes a long time to lyse pathogens, and the nucleic acid is not completely released, which affects the sensitivity.

[0004] The second method: conventional PCR instrument + small ultrasonic instrument + manual sampler quantitative addition method: the release agent containing the sample is first treated with a small ultrasonic instrument, and then the operator uses the sampler to manually add the release agent containing the sample to the reaction tube for quantitative analysis, and then the test is performed on the machine. This method has the disadvantages of requiring two devices, having many operation steps, taking a long time, and being unable to achieve rapid detection.

[0005] It can be seen that in order to accelerate the release of nucleic acids in samples and increase the nucleic acid release rate, it is usually necessary to use a small ultrasonic instrument to pre-treat the release agent containing the sample, and then manually use a pipette to quantitatively add samples, which not only makes the entire operation process cumbersome and time-consuming, but also easily introduces addition errors. Summary of the invention

[0006] (I) The problem to be solved by the present invention is that in the prior art, a small ultrasonic instrument is usually required to pre-treat the release agent containing the sample, and then a sampler is required to manually perform quantitative sample addition, which not only makes the entire operation process cumbersome and time-consuming, but also easily introduces sample addition errors.

[0007] (II) Technical solution

[0008] A molecular detection device with sample heating and quantitative sample addition functions, comprising a base, an optical collection system and at least one detection module, wherein the detection module comprises a detection cavity, a drive system, a fluid control system and an auxiliary heating system;

[0009] The detection module is arranged on the base, and a socket for inserting a reagent card is formed on the top of the detection cavity; the drive system, the fluid control system and the auxiliary heating system are arranged in sequence in the detection cavity, and the socket is located between the fluid control system and the auxiliary heating system;

[0010] The fluid control system comprises a machine block, a plurality of fluid incubation drive mechanisms, and a plurality of fluid barrier mechanisms, wherein the fluid incubation drive mechanisms and the fluid barrier mechanisms are arranged on the machine block in sequence and spaced apart in a vertical direction, and the fluid incubation drive mechanisms and the fluid barrier mechanisms can slide toward the auxiliary heating system, and each of the fluid incubation drive mechanisms is provided with a heating module for heating the reagent card;

[0011] The driving system is used to drive the fluid blocking mechanism and the fluid incubation driving mechanism to approach or move away from the auxiliary heating system; the auxiliary heating system includes an auxiliary heating box, and the auxiliary heating box is formed with auxiliary heating areas corresponding to the fluid incubation driving mechanisms on the end surface facing the fluid control system, and each of the auxiliary heating areas is provided with a heating module for heating the reagent card; at least one of the auxiliary heating areas is provided with a light-transmitting hole for receiving and transmitting optical signals;

[0012] The optical acquisition system is used to acquire and process optical signals.

[0013] According to one embodiment of the present invention, the driving system includes a plurality of first driving members and a plurality of second driving members, the first driving members correspond to the fluid blocking mechanism one by one, and the first driving members are connected to the fluid blocking mechanism to drive the fluid blocking mechanism to move closer to or away from the auxiliary heating system to isolate the liquid in the reagent card;

[0014] The second driving member corresponds to the fluid incubation driving mechanism one by one, and is connected to the fluid incubation driving mechanism to drive the fluid incubation driving mechanism to move toward or away from the auxiliary heating system to squeeze the reagent card.

[0015] According to an embodiment of the present invention, at least three fluid barrier mechanisms are provided, and at least two fluid incubation drive mechanisms are provided, and the fluid incubation drive mechanism is provided between two adjacent fluid barrier mechanisms.

[0016] According to an embodiment of the present invention, at least two auxiliary heating regions are provided, the two auxiliary heating regions are respectively a first auxiliary heating region and a second auxiliary heating region, the two auxiliary heating regions are arranged in a vertical direction, the first auxiliary heating region is higher than the second auxiliary heating region, and the light transmission hole is provided in the second auxiliary heating region;

[0017] The heating module in the auxiliary heating area includes at least one temperature sensor and at least one heating plate or cooling plate.

[0018] According to an embodiment of the present invention, a plurality of the detection modules are provided, and the plurality of the detection modules are arranged in sequence along the length direction of the base.

[0019] According to one embodiment of the present invention, the optical acquisition system includes a scanning head module and a transmission module, the scanning head module is installed on the transmission module, and the transmission module is used to drive the scanning head module to reciprocate along the length direction of the base to collect optical signals from multiple reagent cards in the detection modules.

[0020] According to one embodiment of the present invention, the detection module further includes a pressure cover, which is arranged on the top of the detection cavity and is used to close the socket.

[0021] According to one embodiment of the present invention, the heating module in the fluid incubation drive mechanism includes at least one temperature sensor for temperature control and at least one heating plate or cooling plate.

[0022] A molecular detection system comprises the above-mentioned molecular detection device with sample heating and quantitative sample addition functions and a reagent card, wherein the reagent card comprises a bracket, a hose and a U-shaped support plate, wherein a sampling port for reagent entry is provided at the top of the bracket, one end of the hose is connected to the bottom outlet of the sampling port, and the other end thereof is connected to the inner bottom wall of the support plate; a plug mechanism for plugging the sampling port is provided on the bracket, and the bracket and the two ends of the U-shaped support plate are clamped together; a semi-seal is provided in the hose which is opened under external pressure, and freeze-dried beads are wrapped inside the hose.

[0023] According to one embodiment of the present invention, the plug mechanism comprises a plastic belt and a plug connected in sequence, an end of the plastic belt away from the plug is connected to the side of the injection port, and at least one sealing ring is provided on the side of the plug.

[0024] A molecular detection system comprises the above-mentioned molecular detection device with sample heating and quantitative sample addition functions and a reagent card, wherein the reagent card comprises a bracket, a hose and a U-shaped support plate, wherein a sampling port for reagent entry is provided at the top of the bracket, one end of the hose is connected to the bottom outlet of the sampling port, and the other end thereof is connected to the inner bottom wall of the support plate; a plug mechanism for plugging the sampling port is provided on the bracket, and the bracket and the two ends of the U-shaped support plate are clamped together; a semi-seal is provided in the hose which is opened under external pressure, and freeze-dried beads are wrapped inside the hose.

[0025] According to one embodiment of the present invention, the plug mechanism comprises a plastic belt and a plug connected in sequence, an end of the plastic belt away from the plug is connected to the side of the injection port, and at least one sealing ring is provided on the side of the plug.

[0026] Beneficial effects of the present invention:

[0027] Compared with the existing molecular detection devices, the molecular detection device with sample heating and quantitative sample addition functions has at least the following advantages:

[0028] First, the ultrasonic instrument is omitted, and the reagent samples used for amplification are heated by the fluid control system and the auxiliary heating system, eliminating the need for manual operation and reducing human errors. With the same effect, the operation steps and operation time are reduced.

[0029] Second, the sample is quantitatively added to the reaction solution, eliminating the need for a pipette, avoiding the inaccuracy of manual pipetting, and ensuring the repeatability and reliability of each experiment. The accuracy and consistency of each addition are guaranteed through precise sample volume control and preset programs.

[0030] Third, the functions of heating, quantitative sample addition, and amplification are integrated into an integrated device to simplify the operation process and meet the needs of POCT nucleic acid testing. The integrated design of the device reduces the switching and operation steps between different devices and improves the overall operation efficiency.

[0031] Fourth, the sample volume is controlled by extrusion, and finally mixed with the amplification reagent for amplification, so that the entire PCR operation and reaction time can be completed within 10 minutes, greatly improving the detection efficiency.

[0032] The present invention effectively solves the problems of complicated operation, long time and large error in the prior art through innovative sample processing methods and integrated design, and can quickly complete sample PCR detection with only one step of sample addition, without any pipetting steps and additional manual operations. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0034] Figure 1 A structural diagram of a reagent card provided by an embodiment of the present invention;

[0035] Figure 2 A structural diagram of a nucleic acid detector provided by an embodiment of the present invention;

[0036] Figure 3 A structural diagram of a detection module provided by an embodiment of the present invention;

[0037] Figure 4 A front view of a fluid control system provided by an embodiment of the present invention;

[0038] Figure 5 A front view of an auxiliary heating system provided by an embodiment of the present invention;

[0039] Figure 6 A structural diagram of an optical acquisition system provided by an embodiment of the present invention;

[0040] Figure 7 A schematic diagram of the optical orientation of a scanning head module provided in an embodiment of the present invention;

[0041] Figure 8 A diagram showing the positional relationship of the reagent card, fluid control system and auxiliary heating system provided in an embodiment of the present invention during sample quantification and sample heating.

[0042] Icons: 1. Reagent card; 11. Bracket; 111. Inlet; 112. Plug; 1121. Sealing ring; 113. Buckle; 12. Hose; 121. Bottom seal; 122. Half seal; 123. Freeze-dried beads; 13. Support plate; 131. Card slot; 2. Nucleic acid detector; 21. Detection module; 211. Drive system; 212. Fluid control system; 2121. Fluid incubation drive mechanism; 21211. First fluid incubation drive mechanism; 21212. Second fluid incubation drive mechanism; 2122. Fluid barrier mechanism; 21221. First fluid barrier mechanism; 21222. Second fluid barrier mechanism; 21223. First Three-fluid barrier mechanism; 213, auxiliary heating system; 2131, light-transmitting hole; 2132, auxiliary heating area; 21321, first auxiliary heating area; 21322, second auxiliary heating area; 214, pressure cover; 215, single module control circuit; 22, optical acquisition system; 221, scanning head module; 2211, excitation light source; 2212, excitation light lens; 2213, laser light filter; 2214, dichroic mirror; 2215, emission lens; 2216, emission filter; 2217, fluorescence collector; 222, transmission module; 23, control system; 24, base; a, required volume sample; b, excess volume sample. DETAILED DESCRIPTION

[0043] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Embodiment 1:

[0045] like Figure 2-Figure 8As shown, embodiment 1 of the present invention provides a molecular detection device with sample heating and quantitative sample addition functions, specifically a nucleic acid detector 2, which includes a base 24, an optical acquisition system 22 and at least one detection module 21, the detection module 21 includes a detection cavity, a drive system 211, a fluid control system 212 and an auxiliary heating system 213; the detection module is arranged on the base 24, and a socket for inserting a reagent card 1 is formed on the top of the detection cavity; the drive system 211, the fluid control system 212 and the auxiliary heating system 213 are arranged in sequence in the detection cavity, and the socket is located between the fluid control system 212 and the auxiliary heating system 213; the fluid control system 212 includes a machine block, a plurality of fluid incubation drive mechanisms 2121, and a plurality of fluid barrier mechanisms 2122, and the fluid incubation drive mechanisms 2121 and the fluid barrier mechanisms 2122 are arranged in sequence in the vertical direction. On the machine block, the fluid incubation drive mechanism 2121 and the fluid barrier mechanism 2122 can slide toward the auxiliary heating system 213, and each of the fluid incubation drive mechanisms 2121 is provided with a heating module for heating the reagent card 1; the drive system 211 is used to drive the fluid barrier mechanism 2122 and the fluid incubation drive mechanism 2121 to approach or move away from the auxiliary heating system 213; the auxiliary heating system 213 includes an auxiliary heating machine box, and the auxiliary heating machine box is formed with auxiliary heating areas 2132 corresponding to the fluid incubation drive mechanisms 2121 on the end surface facing the fluid control system 212, and each auxiliary heating area 2132 is provided with a heating module for heating the reagent card 1; at least one auxiliary heating area 2132 is provided with a light-transmitting hole 2131 for receiving and transmitting optical signals; the optical acquisition system 22 is used to collect and process optical signals.

[0046] The reagent card 1 used in this embodiment is as follows Figure 1 As shown, it includes a bracket 11, a hose 12 and a U-shaped support plate 13, wherein a flow channel penetrating the bracket 11 is provided on the upper surface of the bracket 11, and an injection port 111 connected to the flow channel is provided on the upper surface of the bracket 11, the top of the hose 12 is open, the top of the hose 12 is connected to the flow channel of the bracket 11, and the bottom end of the hose 12 has a bottom seal 121, and the bottom seal 121 of the bottom end of the hose 12 is connected to the inner bottom wall of the support plate 13. Figure 8 As shown, the bottom end of the hose 12 is wrapped with freeze-dried beads 123, and a semi-sealed opening 122 is provided inside the hose 12. The semi-sealed opening 122 can be opened under external pressure without damaging the hose 12. Furthermore, a plug mechanism for plugging the injection port 111 is provided on the bracket 11.

[0047] When using the molecular detection device with sample heating and quantitative sample addition functions to conduct an amplification experiment, first dilute PIV1 with PBS (polybutylene succinate), take a certain amount of the diluent and add it to the extraction bottle containing the release agent, mix well, and then drop a fixed amount of the diluent into the injection port 111 of the reagent card 1 containing freeze-dried beads 123 and a semi-seal 122, and then close the plug mechanism. Then, the reagent card 1 is inserted into the socket of the detection cavity, and then the driving system 211 is used to drive different fluid incubation driving mechanisms 2121 and fluid barrier mechanisms 2122 to cooperate with each other, so that the excess sample in the hose 12 is isolated to the top of the hose 12 and out of the temperature control area, and the sample quantification is completed. Then, the heating module in the fluid incubation driving mechanism 2121 and the heating module in the auxiliary heating area 2132 cooperate to preheat the quantified sample to improve the release efficiency of the nucleic acid in the sample. Then, the driving system 211 is used to drive the fluid incubation driving mechanism 2121 to squeeze the heated sample into the freeze-dried bead 123 area below, and squeeze it up and down to mix it evenly with the freeze-dried beads 123. During this process, the optical collection system 22 continuously collects optical signals through the light-transmitting hole 2131, collects multiple sets of fluorescence data, and obtains the amplification curve results.

[0048] Compared with the existing molecular detection devices, the molecular detection device with sample heating and quantitative sample addition functions has at least the following advantages:

[0049] First, the ultrasonic instrument is omitted, and the reagent sample used for amplification is heated by the fluid control system 212 and the auxiliary heating system 213, without manual operation, thus reducing human errors. Under the condition of the same effect, the operation steps and operation time are reduced.

[0050] Second, the sample is quantitatively added to the reaction solution, eliminating the need for a pipette, avoiding the inaccuracy of manual pipetting, and ensuring the repeatability and reliability of each experiment. The accuracy and consistency of each addition are guaranteed through precise sample volume control and preset programs.

[0051] Third, the functions of heating, quantitative sample addition, and amplification are integrated into an integrated device to simplify the operation process and meet the needs of POCT nucleic acid testing. The integrated design of the device reduces the switching and operation steps between different devices and improves the overall operation efficiency.

[0052] Fourth, the sample volume is controlled by extrusion, and finally mixed with the amplification reagent for amplification, so that the entire PCR operation and reaction time can be completed within 10 minutes, greatly improving the detection efficiency.

[0053] In summary, the present invention effectively solves the problems of complicated operation, long time and large errors in the prior art through innovative sample processing methods and integrated design, and can quickly complete sample PCR detection with only one step of sample addition, without any pipetting steps and additional manual operations.

[0054] Among them, PIV1 is human parainfluenza virus type 1, which belongs to the Paramyxoviridae family and is a single-stranded RNA virus.

[0055] As a specific example, Figure 3 As shown, the detection module is arranged on the base 24, and the driving system 211, the fluid control system 212 and the auxiliary heating system 213 are arranged in sequence in the detection cavity, and the socket is located between the fluid control system 212 and the auxiliary heating system 213. Among them, the fluid incubation driving mechanism 2121 in the fluid control system 212 is in the shape of a plate, and there are two of them. For the convenience of distinction, the two fluid incubation driving mechanisms 2121 are named as the first fluid incubation driving mechanism 21211 and the second fluid incubation driving mechanism 21212 in order from high to low. There are three fluid blocking mechanisms 2122 in the fluid control system 212, and the fluid blocking mechanisms 2122 are in the shape of a block. In order from high to low, the three fluid blocking mechanisms 2122 are named as the first fluid blocking mechanism 21221, the second fluid blocking mechanism 21222 and the third fluid blocking mechanism 21223. Furthermore, the first fluid incubation driving mechanism 21211 is located between the first fluid barrier mechanism 21221 and the second fluid barrier mechanism 21222 , and the second fluid incubation driving mechanism 21212 is located between the second fluid barrier mechanism 21222 and the third fluid barrier mechanism 21223 .

[0056] The first fluid incubation drive mechanism 21211, the second fluid incubation drive mechanism 21212, the first fluid barrier mechanism 21221, the second fluid barrier mechanism 21222 and the third fluid barrier mechanism 21223 are respectively slidably mounted on the machine block of the fluid control system 212. Further, the drive system 211 includes three first drive members and two second drive members, the three first drive members correspond to the three fluid barrier mechanisms 2122 one by one, the first drive members are connected to the corresponding fluid barrier mechanisms 2122, and are used to drive the fluid barrier mechanisms 2122 to approach or move away from the auxiliary heating system 213 to isolate the sample in the reagent card 1; the two second drive members correspond to the two fluid incubation drive mechanisms 2121 one by one, and the second drive members are connected to the corresponding fluid incubation drive mechanisms 2121, and are used to drive the fluid incubation drive mechanisms 2121 to approach or move away from the auxiliary heating system 213 to squeeze the reagent card 1.

[0057] Correspondingly, such as Figure 5As shown, an auxiliary heating area 2132 corresponding to the fluid incubation drive mechanism 2121 is formed on the end surface of the auxiliary heating box facing the fluid control system 212, that is, two auxiliary heating areas 2132 are provided. In order from high to low, the two auxiliary heating areas 2132 are named first auxiliary heating area 21321 and second auxiliary heating area 21322. A heating module for preheating the reagent card 1 is provided in each auxiliary heating area 2132.

[0058] Specifically, Figure 8 As shown, first dilute PIV1 1000 times with PBS, take 100 μL and add it to the extraction bottle containing the release agent, mix well, drip 2 drops (about 50ul) into the reagent card 1 containing the freeze-dried beads 123 and the semi-sealed 122, and close the plug mechanism, and then insert the reagent card 1 into the socket of the detection chamber. The detection module 21 executes the steps according to the program. First, the second fluid barrier mechanism 21222 located in the middle moves to the left under the drive of the first driving member to compress the semi-sealed 122 of the hose 12. At the same time, the first fluid incubation driving mechanism 21211 above moves a set distance under the drive of the second driving member to squeeze the sample in the hose 12. The first fluid barrier mechanism 21221 located at the top compresses the hose 12 to the left. At this time, the excess volume sample b of about 30ul is isolated above the hose 12 and leaves the temperature control area. The required volume sample a of about 20ul is quantitatively completed.

[0059] Finally, the heating module in the first fluid incubation drive mechanism 21211 and the heating module in the first auxiliary heating area 21321 heat the required volume sample a to 95°C and continue heating for 1 minute according to the set program to improve the release efficiency of the nucleic acid in the sample. Then the second fluid barrier mechanism 21222 withdraws the set distance, and the first fluid incubation drive mechanism 21211 squeezes the hose 12 again to squeeze the required volume sample a into the freeze-dried beads 123 area below; then the heating module in the second fluid incubation drive mechanism 21212 and the heating module in the second auxiliary heating area 21322 heat the required volume sample a to 55°C and keep it for 2s according to the program, and then the second fluid incubation drive mechanism 21212 squeezes the required volume sample a into the upper area, so that the required volume sample a and the freeze-dried beads 123 are evenly mixed by squeezing up and down until the cycle is 40 times.

[0060] After each volume sample a is squeezed into the area where the freeze-dried beads 123 are located and stays there for 2 seconds, the optical collection system 22 collects optical signals through the light-transmitting holes 2131. 40 sets of fluorescence data are collected and the amplification curve results are obtained.

[0061] In this embodiment, since the first fluid incubation driving mechanism 21211 moves a set distance each time under the drive of the second driving member, that is, the squeezing force applied by the first fluid incubation driving mechanism 21211 to the hose 12 is fixed each time, the excess volume sample b discharged each time is about 30ul. In this way, the function of quantitative residual sample is achieved, the inaccuracy of manual pipetting is avoided, and the repeatability and reliability of each experiment are ensured.

[0062] In addition, since the heating module in the first fluid incubation drive mechanism 21211, the heating module in the second fluid incubation drive mechanism 21212, the heating module in the first auxiliary heating area 21321 and the heating module in the second auxiliary heating area 21322 preheat the sample according to the set program, there is no need to use an ultrasonic device for heating, and the entire preheating process is controlled by the program, without the need for manual operation, thereby reducing human errors.

[0063] like Figure 5 and Figure 8 As shown, the light-transmitting hole 2131 is arranged on the second auxiliary heating area 21322 .

[0064] As a preferred embodiment, the heating module in each fluid incubation drive mechanism 2121 includes a temperature sensor, a heating plate and a cooling plate for temperature control. A single module control circuit 215 is provided on each detection module 21, and the temperature sensor, the heating plate and the cooling plate in the fluid incubation drive mechanism 2121 are respectively connected to the single module control circuit 215 by wires, and the temperature sensor, the heating plate and the cooling plate are controlled by an embedded program to realize the preheating work of the reagent card 1.

[0065] Furthermore, the heating module in each auxiliary heating area 2132 includes a temperature sensor, a heating plate and a cooling plate. The temperature sensor, heating plate and cooling plate in the auxiliary heating area 2132 are respectively connected to the single module control circuit 215 by wires, and the temperature sensor, heating plate and cooling plate are controlled by an embedded program.

[0066] As a preferred embodiment, the three first driving members and the two second driving members all adopt micro electric push rods, which are installed on the machine block of the fluid control system 212, and these multiple micro electric push rods are respectively connected to the single module control circuit 215 signal, and the single module control circuit 215 drives the three first driving members and the two second driving members to work.

[0067] In addition, if Figure 3As shown, a top plate is installed at the top opening of each detection cavity, and a socket is opened on the top plate. After the reagent card 1 is inserted from the socket of the top plate, the bracket 11 of the reagent card 1 is placed on the socket. Furthermore, a pressure cover 214 is hinged at the end of the top plate. The pressure cover 214 is equipped with a press-type lock, which is used to press the top of the reagent card 1 after the reagent card 1 is installed, so as to better fix the reagent card 1.

[0068] In this embodiment, if Figure 2 As shown, a plurality of detection modules 21 are provided, and the plurality of detection modules 21 are arranged in sequence along the length direction of the base 24, and the optical collection system 22 is located at the back of the detection module 21, that is, near the position of the pressure cover 214. The optical collection system 22 includes a scanning head module 221 and a transmission module 222, and the scanning head module 221 is installed on the transmission module 222, and the transmission module 222 is used to drive the scanning head module 221 to reciprocate along the length direction of the base 24 to collect the optical signals of the reagent cards 1 in the plurality of detection modules 21.

[0069] Further, such as Figure 6 As shown, the transmission module 222 adopts a belt-type transmission mechanism, and the scanning head module 221 is installed on the belt of the belt-type transmission mechanism. It moves back and forth along the arrangement direction of the scanning head module 221 as the belt rotates. The scanning head module 221 is driven by the belt to reciprocate horizontally within a set range, thereby completing the optical scanning action.

[0070] In addition, the optical collection system 22 further includes a guide rail, which is arranged along the length direction of the base 24. The scanning head module 221 is slidably connected to the guide rail to improve the stability of the scanning head module 221 when moving.

[0071] Preferably, Figure 7 As shown, the scanning head module 221 includes excitation and emission elements of multiple fluorescence detection channels.

[0072] Each fluorescence detection channel at least comprises an excitation light source 2211, an excitation light lens 2212, a laser light filter 2213, a dichroic mirror 2214, an emission lens 2215, an emission filter 2216 and a fluorescence collector 2217;

[0073] Optionally, each fluorescence detection channel may also be designed to include a structure including optical elements such as a collimating lens and a light guide mirror.

[0074] It should be noted that the number of fluorescence detection channels in this embodiment is ≥2. Specifically, the number of fluorescence detection channels may be 4, namely, FAM / VIC / ROX / Cy5 channels.

[0075] Embodiment 2:

[0076] The second embodiment provides a molecular detection system, including a molecular detection device with sample heating and quantitative sample addition functions and a reagent card 1 in the first embodiment, such as Figure 1 As shown, the reagent card 1 includes a bracket 11, a hose 12 and a U-shaped support plate 13. A flow channel penetrating the bracket 11 is provided on the upper surface of the bracket 11, and an injection port 111 connected to the flow channel is provided on the upper surface of the bracket 11. The top of the hose 12 is open, and the top of the hose 12 is connected to the flow channel of the bracket 11. The bottom end of the hose 12 has a bottom seal 121, and the bottom seal 121 at the bottom end of the hose 12 is connected to the inner bottom wall of the support plate 13.

[0077] The bracket 11 and the two ends of the U-shaped support plate 13 are clamped together; a semi-sealed opening 122 which can be opened under external pressure is provided in the hose 12, freeze-dried beads 123 are wrapped inside the hose 12, and a plug mechanism for blocking the sample inlet 111 is provided on the bracket 11.

[0078] Preferably, the bracket 11 is made of a polymer material, and illustratively, can be any one of PTFE, PA, PC, PET, PP, and PMMA, and its color can be transparent or translucent.

[0079] The support plate 13 is made of a polymer material, and illustratively, can be any one of PTFE, PA, PC, PET, PP, and PMMA.

[0080] Exemplarily, the thickness H of the support plate 13 may be in the range of 0.1 mm ≤ H ≤ 3 mm.

[0081] Preferably, the hose 12 is a flexible film with certain elasticity and scalability, and the color can be transparent or translucent. The top of the hose 12 is tightly connected to the bracket 11, and the connection method can be adhesive bonding, welding, injection molding, etc., and there is a bottom seal 121 below it to prevent leakage of internal substances. The bottom seal 121 is connected to the inner wall of the bottom of the support plate 13, and the connection method can be heat sealing, welding, adhesive bonding, etc.

[0082] Exemplarily, the range of the wall thickness h of the hose 12 is: 0.01 mm≤h≤1 mm; the range of the length L is: 2 mm≤L≤200 mm; and the range of the inner diameter d is: 1 mm≤d≤8 mm.

[0083] As a preferred embodiment, the plug mechanism includes a plastic belt and a plug 112 connected in sequence, the end of the plastic belt away from the plug 112 is connected to the side of the injection port 111, and at least one sealing ring 1121 is provided on the side of the plug 112. The sealing ring 1121 cooperates with the plug 112 to seal the injection port 111, thereby ensuring airtightness and preventing environmental pollution caused by evaporation of reagents.

[0084] It should be noted that the support plate 13 and the bracket 11 can be connected in a welding, hot pressing, adhesive bonding, buckle, etc. Preferably, a buckle is used. Exemplarily, the bracket 11 is provided with a buckle 113 for connecting with the support plate 13, and two buckles 113 are provided, and the two buckles 113 are located on both sides of the bottom of the bracket 11. The two ends of the top of the support plate 13 are respectively provided with a slot 131 used in conjunction with the buckle 113.

[0085] In addition, if Figure 2 As shown, the molecular detection system also includes a control system 23, which is arranged in the space below the base 24 and includes a main control circuit and a matching program. The control system 23 is used to trigger related reaction programs when the reagent card 1 is inserted into the instrument, including but not limited to temperature control, movement, optical detection, data analysis and other functions, thereby completing the experiment.

[0086] The molecular detection system also includes a shell (not shown in the figure), which is used for packaging, protection and aesthetics of the outside of the instrument.

[0087] The use of this molecular detection system can complete sample PCR detection within 10 minutes. Only one step of sample addition is required, without any pipetting steps and additional manual operations. The same sample was amplified 10 times, and the CV value was less than 3%. The error caused by the pipetting step was avoided. Therefore, the present invention is combined with the application of sample release agents to effectively reduce the PCR detection time from the original 60-90 minutes to less than 10 minutes. And the whole process only requires one step of sample addition, without the need for cumbersome operations. The PCR detection efficiency is improved, the operator's workload is reduced, and the deviation of the experimental results due to the operator's mistakes is reduced.

[0088] In the description of the present invention, it should be noted that the terms "upper", "lower", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0089] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the inside of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. In addition, in the description of the present invention, unless otherwise specified, "plurality" means two or more.

[0090] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A molecular detection device with sample heating and quantitative sample addition functions, characterized in that: It comprises a base (24), an optical collection system (22) and at least one detection module (21), wherein the detection module (21) comprises a detection chamber, a drive system (211), a fluid control system (212) and an auxiliary heating system (213); The detection module is arranged on the base (24), and a socket for inserting the reagent card (1) is formed on the top of the detection cavity; the drive system (211), the fluid control system (212) and the auxiliary heating system (213) are arranged in sequence in the detection cavity, and the socket is located between the fluid control system (212) and the auxiliary heating system (213); The fluid control system (212) comprises a machine block, a plurality of fluid incubation drive mechanisms (2121), and a plurality of fluid barrier mechanisms (2122); the fluid incubation drive mechanisms (2121) and the fluid barrier mechanisms (2122) are arranged on the machine block in a sequentially spaced manner along a vertical direction, and the fluid incubation drive mechanisms (2121) and the fluid barrier mechanisms (2122) can slide toward the auxiliary heating system (213); and each of the fluid incubation drive mechanisms (2121) is provided with a heating module for heating the reagent card (1); The driving system (211) is used to drive the fluid barrier mechanism (2122) and the fluid incubation driving mechanism (2121) to move toward or away from the auxiliary heating system (213); the auxiliary heating system (213) comprises an auxiliary heating box, and an auxiliary heating area (2132) corresponding to the fluid incubation driving mechanism (2121) is formed on the end surface of the auxiliary heating box facing the fluid control system (212), and each of the auxiliary heating areas (2132) is provided with a heating module for heating the reagent card (1); at least one of the auxiliary heating areas (2132) is provided with a light-transmitting hole (2131) for receiving and transmitting optical signals; The optical acquisition system (22) is used to acquire and process optical signals.

2. A molecular detection device with sample heating and quantitative sample addition functions according to claim 1, characterized in that: The driving system (211) comprises a plurality of first driving members and a plurality of second driving members, wherein the first driving members correspond to the fluid blocking mechanism (2122) one by one, and the first driving members are connected to the fluid blocking mechanism (2122) and are used to drive the fluid blocking mechanism (2122) to move toward or away from the auxiliary heating system (213) so as to isolate the liquid in the reagent card (1); The second driving member corresponds to the fluid incubation driving mechanism (2121) one by one, and the second driving member is connected to the fluid incubation driving mechanism (2121) and is used to drive the fluid incubation driving mechanism (2121) to move toward or away from the auxiliary heating system (213) to squeeze the reagent card (1).

3. A molecular detection device with sample heating and quantitative sample addition functions according to claim 2, characterized in that: At least three fluid barrier mechanisms (2122) are provided, and at least two fluid incubation drive mechanisms (2121) are provided, and the fluid incubation drive mechanism (2121) is provided between two adjacent fluid barrier mechanisms (2122).

4. A molecular detection device with sample heating and quantitative sample addition functions according to claim 3, characterized in that: At least two auxiliary heating regions (2132) are provided, the two auxiliary heating regions (2132) are respectively a first auxiliary heating region and a second auxiliary heating region, the two auxiliary heating regions (2132) are arranged in a vertical direction, the first auxiliary heating region is higher than the second auxiliary heating region, and the light-transmitting hole (2131) is provided in the second auxiliary heating region; The heating module in the auxiliary heating area (2132) includes at least one temperature sensor and at least one heating plate or cooling plate.

5. A molecular detection device with sample heating and quantitative sample addition functions according to claim 4, characterized in that: A plurality of the detection modules (21) are provided, and the plurality of detection modules (21) are arranged in sequence along the length direction of the base (24).

6. A molecular detection device with sample heating and quantitative sample addition functions according to claim 5, characterized in that: The optical acquisition system (22) comprises a scanning head module (221) and a transmission module (222), wherein the scanning head module (221) is mounted on the transmission module (222), and the transmission module (222) is used to drive the scanning head module (221) to move back and forth along the length direction of the base (24) to acquire optical signals of the reagent cards (1) in the plurality of detection modules (21).

7. A molecular detection device with sample heating and quantitative sample addition functions according to claim 1, characterized in that: The detection module (21) further comprises a pressure cover (214), wherein the pressure cover (214) is arranged at the top of the detection cavity and is used to close the socket.

8. The molecular detection device with sample heating and quantitative sample addition functions according to claim 1, characterized in that: The heating module in the fluid incubation drive mechanism (2121) includes at least one temperature sensor for temperature control and at least one heating plate or cooling plate.

9. A molecular detection system, characterized in that: A molecular detection device and a reagent card (1) with sample heating and quantitative sample addition functions as described in any one of claims 1 to 8, wherein the reagent card (1) comprises a bracket (11), a hose (12) and a U-shaped support plate (13); a sample inlet (111) for reagent entry is provided at the top of the bracket (11); one end of the hose (12) is connected to the bottom outlet of the sample inlet (111), and the other end is connected to the inner bottom wall of the support plate (13); a plug mechanism for plugging the sample inlet (111) is provided on the bracket (11), and the bracket (11) and the two ends of the U-shaped support plate (13) are clamped together; a semi-sealed opening (122) opened under external pressure is provided inside the hose (12), and freeze-dried beads are wrapped inside the hose (12).

10. A molecular detection system according to claim 9, characterized in that: The plug mechanism comprises a plastic belt and a plug (112) connected in sequence, wherein one end of the plastic belt away from the plug (112) is connected to a side surface of the injection port (111), and at least one sealing ring (1121) is provided on the side surface of the plug (112).

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