Composition and application thereof in preparation of whitening skin care product

Abstract

The invention relates to the technical field of cosmetics, in particular to a composition and application thereof in preparation of whitening skin care products. The composition provided by the invention comprises the kapok extract, the rose extract, the China rose extract and the lotus seed pot extract, and the formed composition has the effect of inhibiting tyrosinase activity. When the extract is added into cosmetics as a cosmetic additive, the generation of melanin is reduced, so that the whitening effect is achieved. Experiments show that the melanin can be remarkably reduced within 14 days after gel containing the composition is smeared.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a composition and application thereof in preparing whitening skin care products. Background Art

[0002] In the pursuit of beauty, skin whitening has always been the focus of many people. In the process of skin whitening, tyrosinase plays a vital role. Tyrosinase is an oxidase. As the rate-limiting enzyme that regulates melanin production, it is deeply involved in the complex process of melanin production. Specifically, the enzyme participates in two key reactions in the melanin production process. First, the monophenol is hydroxylated into diphenol, which starts the first important transformation of melanin production. Second, the o-diphenol is oxidized into o-diquinone, a reaction that further promotes the process of melanin production. After several subsequent reactions, o-diquinone eventually forms melanin, which is precipitated in the skin, causing the skin color to darken.

[0003] In order to achieve the purpose of skin whitening, two strategies are currently used to inhibit the formation of melanin. Strategy 1 is to prevent the formation of melanin, that is, to reduce the production of melanin from the source by inhibiting the activity of tyrosinase. Strategy 2 is to promote the excretion of melanin that has been generated and accelerate the metabolic process of melanin. Among these two strategies, strategy 1 is currently the key research direction for the development of whitening products. Many whitening cosmetics often add some raw materials that can inhibit the activity of tyrosinase, such as kojic acid, hydroquinone, etc. Kojic acid has a certain ability to inhibit the activity of tyrosinase. It can bind to tyrosinase and prevent the catalytic effect of tyrosinase on the substrate, thereby reducing the formation of melanin. Hydroquinone is also a commonly used whitening ingredient. Its mechanism of action is mainly to reduce the formation of melanin by inhibiting the activity of tyrosinase. However, these whitening cosmetic additives have many serious side effects during long-term use. For example, long-term use of whitening cosmetics containing kojic acid, hydroquinone and other ingredients can easily cause skin sensitivity. While inhibiting the activity of tyrosinase, these ingredients may also interfere with the normal physiological functions of the skin, causing discomfort such as redness, swelling, itching, and tingling. For people with sensitive skin, this side effect may be more obvious and may even cause skin diseases such as allergic dermatitis.

[0004] In contrast, adding plant extracts to cosmetics has many advantages. Chinese herbal medicine has a long history and abundant resources in my country. Many Chinese herbal ingredients have natural whitening effects with relatively few side effects.

[0005] On the one hand, Chinese herbal ingredients usually have mild properties. For example, some Chinese herbal medicines such as Atractylodes macrocephala, White Peony Root, White Poria cocos, etc., have the effects of strengthening the spleen and removing dampness, nourishing blood and whitening. These Chinese herbal ingredients achieve the effect of whitening by regulating the internal environment of the human body and improving the blood circulation of the skin. Compared with chemically synthesized whitening ingredients, Chinese herbal ingredients are less irritating to the skin and are not likely to cause skin sensitivity and other problems.

[0006] On the other hand, Chinese herbal ingredients often have a multi-target mechanism of action. Unlike a single chemical whitening ingredient that mainly inhibits tyrosinase activity, Chinese herbal ingredients can exert whitening effects through multiple pathways. For example, some Chinese herbal ingredients can inhibit the proliferation of melanocytes and reduce the synthesis of melanin; at the same time, they can also promote skin metabolism and accelerate the excretion of melanin. In addition, some Chinese herbal ingredients also have antioxidant effects, which can scavenge free radicals and reduce the damage of oxidative stress to the skin, thereby indirectly achieving a whitening effect.

[0007] However, cosmetics containing plant ingredients still have some defects. For example, the whitening effect of Chinese herbal ingredients may be relatively slow. Compared with chemically synthesized whitening ingredients, Chinese herbal ingredients usually require a longer period of use to show obvious whitening effects. Therefore, further research and development of Chinese herbal extracts with whitening effects has positive significance. Summary of the invention

[0008] In view of this, the technical problem to be solved by the present invention is to provide a composition and its application in the preparation of whitening skin care products.

[0009] The composition provided by the present invention is composed of the following components in parts by weight:

[0010] 10-20 parts of kapok flower extract;

[0011] 5-10 parts of rose extract;

[0012] 5-15 parts of rose extract;

[0013] 10-20 parts of lotus seed pod extract.

[0014] In some embodiments, the composition consists of 10 parts of kapok flower extract, 10 parts of rose flower extract, 10 parts of rose flower extract and 10 parts of lotus seed pod extract.

[0015] In other embodiments, the composition consists of 15 parts of kapok flower extract, 10 parts of rose flower extract, 5 parts of rose flower extract and 20 parts of lotus pod extract.

[0016] In other embodiments, the composition consists of 15 parts of kapok flower extract, 5 parts of rose flower extract, 15 parts of rose flower extract and 15 parts of lotus pod extract.

[0017] In the present invention, the kapok flower extract is obtained by reflux extraction of kapok flowers from ethanol aqueous solution. In this method, the volume fraction of ethanol in the ethanol aqueous solution is 80% to 95%; for example, the volume fraction of ethanol is 80%, 85%, 90% or 95%. The volume of the ethanol aqueous solution is 10 to 20 times that of the kapok flower; for example, the volume of the ethanol aqueous solution is 10 times, 15 times or 20 times that of the kapok flower. In the present invention, the reflux extraction is Soxhlet reflux extraction, and the extraction conditions include: the heating level in the extraction stage is 16, and the extraction time is 3h; the heating level in the drying stage is 10, and the drying time is 20min.

[0018] In the present invention, the rose extract is obtained by reflux extraction of rose from ethanol-water solution. In this method, the volume fraction of ethanol in the ethanol-water solution is 80% to 95%; for example, the volume fraction of ethanol is 80%, 85%, 90% or 95%. The volume of the ethanol-water solution is 10 to 20 times that of the kapok flower; for example, the volume of the ethanol-water solution is 10 times, 15 times or 20 times that of the kapok flower. In the present invention, the reflux extraction is Soxhlet reflux extraction, and the extraction conditions include: the heating level in the extraction stage is 16, and the extraction time is 3h; the heating level in the drying stage is 10, and the drying time is 20min.

[0019] In the present invention, the rose extract is obtained by reflux extraction of rose from ethanol aqueous solution. In this method, the volume fraction of ethanol in the ethanol aqueous solution is 80% to 95%; for example, the volume fraction of ethanol is 80%, 85%, 90% or 95%. The volume of the ethanol aqueous solution is 10 to 20 times that of the kapok flower; for example, the volume of the ethanol aqueous solution is 10 times, 15 times or 20 times that of the kapok flower. In the present invention, the reflux extraction is Soxhlet reflux extraction, and the extraction conditions include: the heating level in the extraction stage is 16, and the extraction time is 3h; the heating level in the drying stage is 10, and the drying time is 20min.

[0020] In the present invention, the lotus pod extract is obtained by reflux extraction of lotus pods with ethanol-water solution. In this method, the volume fraction of ethanol in the ethanol-water solution is 80% to 95%; for example, the volume fraction of ethanol is 80%, 85%, 90% or 95%. The volume of the ethanol-water solution is 10 to 20 times that of the kapok flower; for example, the volume of the ethanol-water solution is 10 times, 15 times or 20 times that of the kapok flower. In the present invention, the reflux extraction is Soxhlet reflux extraction, and the extraction conditions include: the heating level in the extraction stage is 16, and the extraction time is 3h; the heating level in the drying stage is 10, and the drying time is 20min.

[0021] The invention also provides application of the composition in preparing whitening products.

[0022] In an embodiment of the present invention, the efficacy of the whitening product includes inhibiting tyrosinase activity and / or inhibiting melanin levels.

[0023] Furthermore, the present invention also provides a whitening product, which comprises the above-mentioned composition and auxiliary materials acceptable in cosmetics.

[0024] In the present invention, the concentration of the composition in the whitening product is 0.25-1 mg / mL.

[0025] In the present invention, the auxiliary materials include: a thickener, a moisturizer and a pH adjuster;

[0026] In the present invention, the thickener is selected from at least one of carbomer, xanthan gum, hydroxyethyl cellulose, hydroxypropyl methylcellulose, gum arabic, guar gum, sodium alginate, agar, pectin, polyvinyl pyrrolidone, polyethylene glycol, methyl cellulose, sodium carboxymethyl cellulose, locust bean gum, konjac gum, candelilla wax, microcrystalline wax, cetyl alcohol or stearyl alcohol;

[0027] In the present invention, the moisturizing agent is selected from at least one of butylene glycol, glycerin, hyaluronic acid, propylene glycol, sorbitol, polyglutamic acid, ceramide, urea, xylitol, trehalose, chitosan, sodium lactate, PCA-sodium, collagen, squalene, jojoba oil, olive oil, vaseline, and lanolin;

[0028] In the present invention, the pH adjuster is selected from at least one of citric acid, sodium citrate, lactic acid, sodium lactate, sodium hydroxide, potassium hydroxide, triethanolamine, phosphoric acid, sodium dihydrogen phosphate, disodium hydrogen phosphate, boric acid, borax, malic acid, tartaric acid, sodium tartrate, potassium carbonate, sodium carbonate, aminosulfonic acid, and sodium bicarbonate.

[0029] The whitening product described in the present invention is lotion, face cream, eye cream, hand cream, essence, toner, softener, mask liquid, cleanser, cleansing cream, cleansing milk, cleansing cream, make-up remover oil, make-up remover water, make-up remover milk, gel, pressed powder or loose powder.

[0030] In some specific embodiments, the whitening product includes 0.012 g / mL of carbomer, 0.1 g / mL of butylene glycol, 0.001 g / mL of triethanolamine, 0.004 g / mL of the composition of any one of claims 1 to 3, and water.

[0031] Furthermore, the present invention also provides a method for preparing the whitening product, comprising: mixing the composition with an auxiliary material acceptable in cosmetics to obtain the whitening product.

[0032] Furthermore, the present invention also provides a whitening method, which comprises administering the whitening product as described above to a subject.

[0033] The present invention provides a composition comprising kapok flower extract, rose flower extract, rose flower extract and lotus pod extract, and the resulting composition has the function of inhibiting tyrosinase activity. The extract is added to cosmetics as a cosmetic additive to reduce the generation of melanin, thereby exerting a whitening effect. Experiments show that applying a gel containing the composition of the present invention can achieve a significant reduction in melanin within 14 days. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 Shows the changes in melanin levels in people who tried the whitening gel (vs D0, **p < 0.01). DETAILED DESCRIPTION

[0035] The present invention provides a composition and its application in the preparation of whitening skin care products. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the method and application of this article without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0036] Unless otherwise defined herein, scientific and technical terms related to the present invention shall have the meanings understood by those of ordinary skill in the art.

[0037] In this application, the term "and / or" describes the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. A and B can be singular or plural.

[0038] In this application, "at least one" means one or more, "more than one" means two or more. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single or plural items.

[0039] The terms "include", "comprising" and "having" are used interchangeably herein and are intended to indicate the inclusiveness of the solution, meaning that the solution may have other elements in addition to the listed elements. At the same time, it should be understood that the use of "include", "comprising" and "having" descriptions herein also provides a "consisting of..." solution.

[0040] The numerical ranges and parameters involved in the present invention have been presented in the specific embodiments as accurately as possible. However, any numerical value inherently inevitably contains standard deviations due to individual testing methods. Therefore, unless otherwise explicitly stated, it should be understood that all numerical ranges or specific data used in this disclosure may have certain reasonable deviations within a certain range, for example: within ±10%, ±5%, ±1% or ±0.5%.

[0041] Some cases are recorded in the examples and comparative examples of the present invention. The examples show some implementations of the present invention. However, this does not mean that the effects of the present invention can only be achieved in these cases. In fact, any concentration of each component between the two endpoint values ​​shown in the examples can achieve good effects of inhibiting tyrosinase activity and reducing melanin levels, and the best case is Example 1.

[0042] In addition to Examples 1 to 3, many attempts were made during the research and development process, for example: other solvents were used for extraction in the preparation of various extracts, such as water or anhydrous ethanol, or decoction extraction or other auxiliary extraction methods were used, but the effects of the obtained extracts after combination were not as good as those in Examples 1 to 3. In addition, the present invention also tried compositions lacking some components (Comparative Examples 1 to 3), and the results showed that the effects were not as good as those in Examples 1 to 3, indicating that the components in the compositions provided by the present invention cooperate with each other and are indispensable. In addition, the inhibitory effects of individual components on tyrosinase were also detected. After statistical analysis, the formulas of Examples 1 to 3 were calculated to have a synergistic coefficient less than 1 relative to a single component, indicating that they can produce a synergistic effect, and the synergistic effect of Example 1 is more significant. In addition, different ratios also have an effect on the removal of melanin and the inhibition of tyrosinase activity. Examples 1 to 3 present the effects achieved under the best ratios, and other cases with poor effects are not repeated here.

[0043] The test materials used in the present invention are all common commercial products and can be purchased on the market.

[0044] Kapok is the flower of Bombax malabaricum DC., a plant of the Bombacaceae family.

[0045] Rose is the dried flower buds of Rosa rugosa, a plant of the Rosaceae family.

[0046] Rose is the semi-open flower of Rosa chinensis, a plant of the Rosaceae family.

[0047] The lotus pod is the dried receptacle of the lotus Nelumbo nucifera Gaertn. of the Nymphaeaceae family.

[0048] In the present invention, the kapok flower alcohol extract is prepared by the following method: kapok flower medicinal materials are crushed to form kapok flower powder, 10g of kapok flower powder is weighed and placed on filter paper to wrap into a medicine bag, the medicine bag is placed at the bottom of a cylindrical glass sample tube of an extractor, 15 times the volume of 90% ethanol is poured into a solvent cup and fixed at the lower end interface of the Soxhlet extractor, the heating level of the extraction stage is set to 16, the extraction time is 3h; the heating level of the drying stage is set to 10, the drying time is 20min, and extraction is performed. After the extraction is completed, the extract is subjected to vacuum low-temperature freeze-drying to finally obtain the kapok flower alcohol extract.

[0049] The preparation methods of rose flower ethanol extract, rose flower ethanol extract and lotus pod ethanol extract are consistent with the preparation method of kapok flower ethanol extract.

[0050] It should be understood that in various embodiments of the present application, the size of the sequence number of each process does not mean the order of execution, some or all of the steps can be executed in parallel or in sequence, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present application. The present invention is further described below in conjunction with the embodiments:

[0051] Example 1

[0052] Take 10 parts of kapok ethanol extract, 10 parts of rose ethanol extract, 10 parts of rose ethanol extract, and 10 parts of lotus pod ethanol extract, dilute them with DMSO to form 50 mg / mL samples, and then dilute them with phosphate buffer solution with pH=6.8 to 2, 1, 0.5, and 0.25 mg / mL samples.

[0053] Example 2

[0054] Take 15 parts of kapok ethanol extract, 10 parts of rose ethanol extract, 5 parts of rose ethanol extract, and 20 parts of lotus pod ethanol extract, dilute them with DMSO to form 50 mg / mL samples, and then dilute them with pH=6.8 phosphate buffer solution to 0.88, 0.44, 0.22, and 0.11 mg / mL samples.

[0055] Example 3

[0056] Take 15 parts of kapok ethanol extract, 5 parts of rose ethanol extract, 15 parts of rose ethanol extract, and 15 parts of lotus pod ethanol extract, dilute them with DMSO to form 50 mg / mL samples, and then dilute them with pH=6.8 phosphate buffer solution to 1, 0.5, 0.25, and 0.125 mg / mL samples.

[0057] Comparative Example 1 (lack of roses)

[0058] Take 10 parts of kapok ethanol extract, 10 parts of rose ethanol extract, and 10 parts of lotus pod ethanol extract, dilute them with DMSO to form 50 mg / mL samples, and then dilute them with pH=6.8 phosphate buffer solution to 1, 0.5, and 0.25 mg / mL samples.

[0059] Comparative Example 2 (lack of roses)

[0060] Take 10 parts of kapok flower ethanol extract, 10 parts of rose flower ethanol extract, and 10 parts of lotus pod ethanol extract, dilute them with DMSO to form 50 mg / mL samples, and then dilute them with pH=6.8 phosphate buffer solution to 1, 0.5, and 0.25 mg / mL samples.

[0061] Comparative Example 3 (Lotus pod missing)

[0062] Take 10 parts of kapok Soxhlet ethanol extract, 10 parts of rose Soxhlet ethanol extract, and 10 parts of rose ethanol extract, dilute them with DMSO to form 50 mg / mL samples, and then dilute them with pH=6.8 phosphate buffer solution to 1, 0.5, and 0.25 mg / mL samples.

[0063] Comparative Example 4 (Kapok alcohol extract)

[0064] Take 10 portions of Kapok alcohol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with pH=6.8 phosphate buffer solution to 2, 1, 0.5, and 0.25 mg / mL samples. Comparative Example 5 (Rose alcohol extract)

[0065] Take 10 portions of rose alcohol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with a phosphate buffer solution with pH=6.8 to 2, 1, 0.5, and 0.25 mg / mL samples. Comparative Example 6 (Rose alcohol extract)

[0066] Take 10 portions of rose alcohol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with pH=6.8 phosphate buffer solution to 2, 1, 0.5, and 0.25 mg / mL samples.

[0067] Take 10 portions of the lotus pod alcohol extract, dilute it with DMSO to form a 50 mg / mL sample, and then dilute it with a phosphate buffer solution with pH=6.8 to form 2, 1, 0.5, and 0.25 mg / mL samples.

[0068] Efficacy verification

[0069] 1. The effects of Examples 1 to 3 and Comparative Examples 1 to 3 on tyrosinase activity were determined using the mushroom tyrosinase DOPA rate oxidation method. The specific process is as follows:

[0070] 1. Accurately weigh 2.5 mg of L-DOPA and dilute it to 5 mL with pH 6.8 phosphate buffer solution to prepare a 2.55 mM L-DOPA solution. Keep it away from light and prepare it before use.

[0071] 2. Use pH 6.8 phosphate buffer solution to dilute mushroom tyrosinase with an enzyme activity of 2.5 kU / mL into a tyrosinase solution with an enzyme activity of 200 U / mL and set aside.

[0072] 3. Add the reaction solutions shown in Table 1 to the 96-well plate in sequence, the reaction system is 160 μL, incubate at 37 ° C for 10 min, add 40 μL of 2.55 mM L-DOPA to each well, incubate at 37 ° C in the dark for 10 min, then quickly place in a multifunctional microplate reader, measure the OD value at 475 nm, repeat the above operation, parallel measurement 3 times, according to the formula: inhibition rate (%) = [(AB)-(CD)] / (AB)*100%, calculate the effect of the embodiment or comparative example group on tyrosinase activity, where A, B, C, and D are the absorbance values ​​corresponding to each group. Use SPSS software to calculate the mass concentration IC50 value corresponding to the inhibition rate of each embodiment and comparative example when it is 50%. The smaller the IC50 value, the stronger the inhibitory effect of the combination on tyrosinase.

[0073] Table 1 Composition of each reaction solution

[0074]

[0075] 2. Effects of Examples and Comparative Examples and Single Components on Tyrosinase Activity

[0076] 1. Using the formula under item "I", the inhibition rates of Examples 1 to 3 on tyrosinase at three concentrations were calculated. The results are shown in Table 2. As can be seen from Table 2, Examples 1 to 3 all have the effect of inhibiting tyrosinase at three concentrations (the inhibition rates are all greater than 0). The IC 50 It can be seen from the values ​​that the IC 50 The value is the smallest, and the inhibitory effect on tyrosinase is the strongest. The drug-deficient comparison examples under Example 1 are set, including comparison example 1 (lacking rose flowers), comparison example 2 (lacking rose flowers), and comparison example 3 (lacking lotus pods), to see the inhibition of tyrosinase by the drug composition after drug deficiency.

[0077] Table 2 Inhibition rate and IC of tyrosinase in various examples 50 value

[0078]

[0079] 2. Use the formula under "1" to calculate the inhibition rates of Comparative Examples 1 to 3 on tyrosinase at 3 concentrations. The results are shown in Table 3. It can be seen from Table 3 that the comparative examples all have the effect of inhibiting tyrosinase at 3 concentrations (the inhibition rates are all greater than 0). However, compared with Example 1 at the same concentrations of 1, 0.5, and 0.25 mg / mL, the inhibition rates of Comparative Example 1 (lacking rose flower), Comparative Example 2 (lacking Chinese rose flower), and Comparative Example 3 (lacking lotus receptacle) on tyrosinase are all significantly lower than that of Example 1( Figure 1 ), and the IC50 value of Example 1 is less than that of Comparative Example 1 (lacking rose flower), Comparative Example 2 (lacking Chinese rose flower), and Comparative Example 3 (lacking lotus receptacle).

[0080] Table 3 Inhibition rates of each comparative example on tyrosinase and IC 50 value

[0081]

[0082] 3. Use the formula under "1" to calculate the inhibition rates of Comparative Examples 4 to 7 on tyrosinase at 4 concentrations. The results are shown in Table 4. Calculate the synergy index based on the IC 50 values of each single drug component to evaluate the synergy of Examples 1 to 4.

[0083] Table 4 Inhibition rates of each single drug component on tyrosinase and IC 50 value

[0084]

[0085] 4. Synergy index calculation

[0086] Refer to Chou, T.C. Theoretical basis, experimental design, and computerized simulation of synergism and antagonism in drug combination studies. Pharmacol. Rev. 1970, 326, 261–270. to verify the synergistic effect of the composition.

[0087] Record the mass concentration corresponding to the 50% inhibition rate of the kapok extract in Comparative Example 4 as IC 50A , record the mass concentration corresponding to the 50% inhibition rate of the rose flower extract in Comparative Example 5 as IC 50B , record the mass concentration corresponding to the 50% inhibition rate of the Chinese rose flower extract in Comparative Example 6 as IC 50C , record the mass concentration corresponding to the 50% inhibition rate of the lotus receptacle extract in Comparative Example 7 as IC 50D .

[0088] In different embodiments, the IC according to the embodiment 50 The mass concentration of kapok extract was calculated to be IC 50a , the mass concentration of rose extract is IC 50b The mass concentration of rose flower extract is IC 50c The mass concentration of lotus seed pod extract is IC 50d .

[0089] The synergistic index calculation formula of the combined use of the four extracts is:

[0090] CI=IC 50a / IC 50A +IC 50b / IC 50B +IC 50c / IC 50C +IC 50d / IC 50D ,

[0091] When CI=1, it means that kapok flower extract, rose flower extract, rose flower extract and lotus pod extract show an additive effect;

[0092] When CI is less than 1, it means that kapok flower extract, rose flower extract, rose flower extract and lotus pod extract show synergistic effect. The smaller the CI value, the stronger the synergistic effect.

[0093] When CI>1, it means that kapok flower extract, rose flower extract, rose flower extract and lotus pod extract show antagonistic effects. The larger the CI value, the stronger the antagonistic effect.

[0094] It can be seen from Table 2 that the synergistic indexes of Examples 1 to 3 are all less than 1, namely 0.78, 0.82, and 0.91, respectively. The smaller the CI value, the stronger the synergistic effect, indicating that at the ratio of Example 1, the synergistic effect of kapok flower extract, rose flower extract, rose flower extract, and lotus pod extract is the strongest.

[0095] 3. Effect of whitening gel formed by Chinese herbal extract composition on melanin production

[0096] 1. Preparation of whitening gel:

[0097] 0.12 g of carbomer, 1 g of butylene glycol, 0.01 g of triethanolamine, and 10 mL of water were added to the composition of Example 1 (10 parts of kapok extract, 10 parts of rose extract, 10 parts of rose extract, and 10 parts of lotus seed pod extract) to a mass fraction of 0.4%, and mixed together to prepare a whitening gel for later use.

[0098] 2. Evaluation of the whitening effect of whitening gel

[0099] Ten trial participants were recruited, and the arm area was divided into a blank control group and a whitening gel group using tattoo stickers to locate the measurement and application area. The blank control group applied a blank matrix without Example 1 every day, and the whitening gel group applied whitening gel every day, twice a day. The melanin content of the blank control group and the whitening gel group was tested on the 0th day (D0), the 7th day (D7) and the 14th day (D14) of the experiment. Each time, the measurement was performed 7 times, and the average value was calculated. The whitening gel group / blank control group was used as the data for the final statistical analysis. The results are shown as follows: Figure 1 As shown in the figure, when the whitening gel product was tried for 14 days, the melanin content of the trial population was significantly reduced (p < 0.01), indicating that the Chinese herbal extract composition contained in the whitening gel product has a significant effect of reducing melanin and can be developed into whitening cosmetics, including lotions, essences, emulsions, creams, masks, etc.

[0100] The above are only preferred embodiments of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition comprising the following components in parts by weight: 10-15 parts of kapok flower extract; 5-10 parts of rose extract; 5-15 parts of rose extract; 10-20 parts of lotus seed pod extract.

2. The composition according to claim 1, characterized in that The composition comprises 10 parts of kapok flower extract, 10 parts of rose flower extract, 10 parts of rose flower extract and 10 parts of lotus seed pod extract; Or composed of 15 parts of kapok flower extract, 10 parts of rose flower extract, 5 parts of rose flower extract and 20 parts of lotus pod extract; Or it is composed of 15 parts of kapok flower extract, 5 parts of rose flower extract, 15 parts of rose flower extract and 15 parts of lotus seed pod extract.

3. The composition according to claim 1 or 2, characterized in that The kapok flower extract is prepared by extracting kapok flowers from an ethanol aqueous solution through reflux extraction; The rose extract is prepared by reflux extraction of roses from ethanol aqueous solution; The rose extract is prepared by extracting rose from ethanol aqueous solution through reflux extraction; The lotus pod extract is prepared by extracting the lotus pod from an ethanol aqueous solution through reflux extraction.

4. Use of the composition according to any one of claims 1 to 3 in the preparation of whitening products.

5. The use according to claim 4, characterized in that: The whitening product efficacy includes inhibiting tyrosinase activity and / or inhibiting melanin levels.

6. A whitening product, characterized in that: The composition comprises the composition according to any one of claims 1 to 3 and auxiliary materials acceptable in cosmetics.

7. The whitening product according to claim 6, characterized in that: The concentration of the composition according to any one of claims 1 to 3 is 0.25 to 1 mg / mL.

8. The whitening product according to claim 6, characterized in that: The auxiliary materials include: thickener, moisturizer and pH regulator; The thickener is selected from at least one of carbomer, xanthan gum, hydroxyethyl cellulose, hydroxypropyl methylcellulose, gum arabic, guar gum, sodium alginate, agar, pectin, polyvinyl pyrrolidone, polyethylene glycol, methyl cellulose, sodium carboxymethyl cellulose, locust bean gum, konjac gum, candelilla wax, microcrystalline wax, cetyl alcohol or stearyl alcohol; The moisturizing agent is selected from at least one of butylene glycol, glycerin, hyaluronic acid, propylene glycol, sorbitol, polyglutamic acid, ceramide, urea, xylitol, trehalose, chitosan, sodium lactate, PCA-sodium, collagen, squalene, jojoba oil, olive oil, vaseline, and lanolin; The pH regulator is selected from at least one of citric acid, sodium citrate, lactic acid, sodium lactate, sodium hydroxide, potassium hydroxide, triethanolamine, phosphoric acid, sodium dihydrogen phosphate, disodium hydrogen phosphate, boric acid, borax, malic acid, tartaric acid, sodium tartrate, potassium carbonate, sodium carbonate, aminosulfonic acid, and sodium bicarbonate.

9. The whitening product according to any one of claims 6 to 8, characterized in that: include: Carbomer 0.012 g / mL, butylene glycol 0.1 g / mL, triethanolamine 0.001 g / mL, the composition of any one of claims 1 to 3 0.004 g / mL and water.

10. The method for preparing the whitening product according to any one of claims 6 to 9, comprising: The whitening product is prepared by mixing the composition according to any one of claims 1 to 3 with an auxiliary material acceptable in cosmetics.

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