Anti-hair loss and anti-dandruff composition and preparation method thereof

Abstract

The invention provides an anti-hair loss and anti-dandruff composition and a preparation method thereof, and belongs to the technical field of wash supplies. The anti-hair loss and anti-dandruff composition is prepared from the following raw materials: a plant raw material, a plant extract, a caffeine composition and an oil control composition, wherein the plant raw materials comprise the following raw materials in parts by mass: 5-20 parts of ginger root, 5-20 parts of black ginger root, 5-20 parts of alpinia japonica root, 1-3 parts of ligusticum wallichii, 5-10 parts of radix sophorae flavescentis, 2-4 parts of fructus cnidii, 1-5 parts of fructus viticis, 3-5 parts of saponin, 5-8 parts of soapberry peel, 1-5 parts of dandelion, 5-8 parts of polygonum multiflorum, 1-3 parts of mulberry, 3-6 parts of cacumen biotae, 3-6 parts of mulberry leaf, 7-10 parts of purslane and 1-4 parts of mangnolia officinalis; the plant extract is prepared from the following raw materials in parts by mass: 5 to 20 parts of aloe extract, 1 to 10 parts of herba menthae extract, 5 to 20 parts of fructus forsythiae fruit extract, 5 to 20 parts of tea leaf extract, 5 to 20 parts of herba centellae extract and 1 to 10 parts of cordyceps militaris extract. The composition prepared by the invention not only has good anti-dropping and anti-dandruff effects, but also has good stability.

Description

Technical Field

[0001] The invention belongs to the technical field of washing products, and in particular relates to an anti-shedding and anti-dandruff composition and a preparation method thereof. Background Art

[0002] Scalp problems such as dandruff and hair loss often affect people's daily lives. Dandruff is mainly caused by dandruff spores. Dandruff spores are affected by internal and external factors and proliferate in large quantities, destroying the normal metabolism of the scalp. Hair loss refers to the phenomenon of hair loss. Normally shed hair is hair in the catagen and telogen phases. Since the hair entering the catagen and the hair entering the new growth phase are constantly in a dynamic balance, the normal amount of hair can be maintained. Pathological hair loss refers to abnormal or excessive hair loss, and there are many reasons for it, such as androgenic alopecia, neurotic alopecia, endocrine alopecia, nutritional alopecia, physical alopecia, chemical alopecia, infectious alopecia, symptomatic alopecia, congenital alopecia and seasonal alopecia. As one of the commonly used washing and care products, hair composition products can be used to clean the oil, sweat stains, dust, microorganisms, etc. on the hair and scalp. In addition, some hair compositions also have various effects such as anti-hair loss, anti-whitening, hair growth and anti-itching.

[0003] Hair products, as products suitable for daily use and not requiring special care steps, have become a way to solve the above-mentioned dandruff and hair loss problems. At present, for specific scalp and hair problems such as hair loss and dandruff, active ingredients such as zinc, ketoconazole, minoxidil and saw palmetto extract are mainly added to the products. Zinc has antifungal effects and is used for dandruff removal, ketoconazole is used for severe dandruff and seborrheic dermatitis, and minoxidil and saw palmetto extract can promote hair growth and are used to prevent hair loss. However, the use of the above-mentioned active ingredients also brings the following disadvantages: it may cause dryness and irritation of the scalp, the effect varies from person to person, and it may take a long time to see the effect.

[0004] In order to solve the side effects and poor effects caused by the above ingredients, natural plant ingredients are gradually applied to hair products. Plant raw materials and plant extracts come from nature. Most plant ingredients are mild and less irritating to the skin and scalp. They also have multiple functions, which improve the effect of solving problems such as hair loss and dandruff. However, the use of natural plant ingredients also has the following disadvantages: many plant extracts contain substances such as unsaturated fatty acids, which are easily oxidized and affected by the external environment, and have poor stability; the activity of plant ingredients is low, and they need to be compounded to achieve obvious effects, and the formula design is complicated. Summary of the invention

[0005] The purpose of the present invention is to provide an anti-hair loss and anti-dandruff composition and a preparation method thereof, so as to solve the problem that the above-mentioned hair products using natural ingredients have poor stability and poor efficacy.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The first aspect of the present invention provides an anti-hair loss and anti-dandruff composition, which is prepared from the following raw materials: plant raw materials, plant extracts, caffeine compositions and oil control compositions;

[0008] The plant raw materials include the following raw materials by weight: 5-20 parts of ginger root, 5-20 parts of black ginger root, 5-20 parts of Alpinia officinalis root, 1-3 parts of Chuanxiong, 5-10 parts of Sophora flavescens, 2-4 parts of Cnidium monnieri, 1-5 parts of Vitex rotundifolia, 3-5 parts of Gleditsia sinensis, 5-8 parts of Sapindus mukorossi, 1-5 parts of Taraxacum officinale, 5-8 parts of Polygonum multiflorum, 1-3 parts of mulberry, 3-6 parts of Platycladus orientalis leaf, 3-6 parts of mulberry leaf, 7-10 parts of Portulaca oleracea and 1-4 parts of Magnolia officinalis;

[0009] The plant extract includes the following raw materials in parts by mass: 5-20 parts of aloe extract, 1-10 parts of peppermint extract, 5-20 parts of forsythia fruit extract, 5-20 parts of tea extract, 5-20 parts of Centella asiatica extract and 1-10 parts of Cordyceps militaris extract;

[0010] The caffeine composition includes cyclodextrin and caffeine;

[0011] The oil control composition includes disodium azelaate, arginine and water.

[0012] Preferably, the mass ratio of ginger root, black ginger root, Alpinia officinalis root and Ligusticum chuanxiong is (2-6):(2-6):(2-6):1.

[0013] Preferably, the mass ratio of Sophora flavescens, Cnidium monnieri, Vitex rotundifolia, Gleditsia sinensis, Sapindus mukorossi and Taraxacum officinale is (1.5-2.5):(0.8-1.2):1:(1-1.5):(2-3):1.

[0014] Preferably, the mass ratio of Polygonum multiflorum, mulberry, Platycladus orientalis leaf and mulberry leaf is (1.5-2.5):(0.5-0.8):(1-1.5):1.

[0015] Preferably, the mass ratio of aloe vera extract to mint extract is (2-3.5):1, the mass ratio of forsythia fruit extract to tea extract is (1-2.5):1, and the mass ratio of Centella asiatica extract to Cordyceps militaris extract is (3-4.5):1.

[0016] At present, there are many materials that use natural ingredients as compositions. However, due to the complexity of the materials contained in natural ingredients and the complexity of compounding, the effect of adding natural ingredients as active ingredients to hair products is not good. The present invention uses plant raw materials and plant extracts as the main raw materials, and designs the specific components of the plant raw materials and the specific components of the plant extracts. The synergistic effect of each raw material significantly improves the anti-dandruff and anti-dandruff effect of the composition. Analysis of the reasons: The present invention sets the mass ratio of ginger root, black ginger root, Alpinia officinarum root and Chuanxiong to (2-6): (2-6): (2-6): 1. This ratio can ensure that the concentration of gingerol and volatile oil is moderate, which can effectively promote blood circulation without over-stimulating the scalp. At the same time, the blood circulation and blood stasis-removing effect of Chuanxiong can further enhance blood circulation, and jointly achieve the anti-hair loss effect. The mass ratio of Radix Sophorae Flavescentis, Fructus Cnidium, Fructus Viticis, Gleditsiae Sapindus, Fructus Sophorae Mukorossi and Herba Taraxaci is set to (1.5-2.5): (0.8-1.2): 1: (1-1.5): (2-3): 1, which can ensure that the synergistic effect between the components is maximized, and the anti-inflammatory, antifungal and cleaning effects are exerted together, effectively reducing dandruff and itching. The mass ratio of Radix Polygoni Multiflori, Fructus Mulberry, Folium Platycladus Orientalis and Folium Mulberry is set to (1.5-2.5): (0.5-0.8): (1-1.5): 1, which can ensure that the nourishing effect of Radix Polygoni Multiflori, the nourishing effect of Fructus Mulberry, the heat-clearing and detoxifying effects of Folium Platycladus Orientalis and Folium Mulberry are exerted together, and jointly promote hair growth and improve scalp health. The present invention sets the mass ratio of aloe vera extract to mint extract to (2-3.5):1, the mass ratio of forsythia fruit extract to tea extract to (1-2.5):1, and the mass ratio of Centella asiatica extract to Cordyceps militaris extract to (3-4.5):1, thereby ensuring that the synergistic effect between the extracts is maximized, and the extracts jointly exert the effects of moisturizing, anti-inflammatory, repairing and promoting cell regeneration, effectively improving scalp health, reducing dandruff and itching, and promoting hair growth.

[0017] The second aspect of the present invention provides a method for preparing an anti-dandruff and anti-dandruff composition, comprising the following steps:

[0018] (1) mixing plant raw materials according to a ratio, adding water to mix, obtaining a mixed liquid, adding a composite enzymatic hydrolyzing agent and an auxiliary enzymatic hydrolyzing agent to the mixed liquid, performing enzymatic hydrolysis at 30 to 38° C. for 5 to 8 hours, inactivating the enzyme, and obtaining an enzymatic hydrolysis product;

[0019] (2) adding a composite bacterial agent to the enzymatic hydrolysis product, introducing oxygen, fermenting at 28 to 35° C. for 3 to 5 days, sterilizing, and obtaining a fermentation product;

[0020] (3) filtering the fermentation product to obtain a supernatant, heating and concentrating the supernatant to obtain a concentrated solution, and freeze-drying the concentrated solution to obtain a plant enzymatic fermentation product;

[0021] (4) Adding the plant extract, the caffeine composition and the oil control composition to the plant enzymatic fermentation product and mixing them evenly to obtain an anti-hair loss and anti-dandruff composition.

[0022] Preferably, in step (1), the mass ratio of the plant raw material to water is 1:(15-20); the composite enzymatic hydrolyzer comprises cellulase, papain and trehalase, wherein the amount of cellulase in the mixed liquid is 100-150 U / mL, the amount of papain in the mixed liquid is 80-100 U / mL, and the amount of trehalase in the mixed liquid is 110-130 U / mL; the auxiliary enzymatic hydrolyzer comprises n-dodecyl alcohol and n-decanol, the mass ratio of n-dodecyl alcohol to n-decanol is (3-5):1, and the amount of the auxiliary enzymatic hydrolyzer added is 0.1-0.3% of the plant raw material.

[0023] Preferably, in step (2), the composite bacterial agent includes Lactobacillus geosporum, Bacillus subtilis, Bacillus cereus and Lactobacillus plantarum, wherein the amount of Lactobacillus geosporum in the enzymatic hydrolysate is 3.5×10 9 ~4.2×10 9 CFU / mL, the amount of Bacillus subtilis in the enzymatic hydrolysate was 5.8×10 9 ~6.5×10 9 CFU / mL, the amount of Bacillus cereus in the enzymatic hydrolysate was 4.5×10 9 ~5.5×10 9 CFU / mL, the amount of Lactobacillus plantarum in the enzymatic hydrolysate was 1.2×10 9 ~1.5×10 9 CFU / mL.

[0024] In order to further improve the anti-dandruff effect and to improve the stability of the composition, and to avoid the precipitation of undecomposed macromolecular substances in the product due to environmental changes during subsequent use, which reduces the stability, the present invention performs a specific enzymatic hydrolysis process and a fermentation process on the plant raw materials, thereby improving the efficiency of plant raw material fermentation and the stability of the plant raw materials added to the product. Analysis of raw materials: In the present invention, three enzymatic agents are used for enzymatic hydrolysis at a specific addition ratio. The antioxidant substances produced during the enzymatic hydrolysis process can prevent the oxidation of unsaturated fatty acids and antioxidants in the plant extract, thereby extending the shelf life of the product; enzymatic hydrolysis can also decompose macromolecular substances, making the texture of the composition more delicate and improving the use experience of the product. The present invention uses four strains to ferment at a specific addition ratio. Lactobacillus geosporum can produce a variety of enzymes and metabolites, further decompose macromolecular substances in plant raw materials, and release more active ingredients; Bacillus subtilis has a strong degradation ability, can decompose complex organic matter, and produce beneficial metabolites; Bacillus cereus can produce antibacterial substances, inhibit the growth of harmful microorganisms, and maintain a healthy environment of the scalp; Lactobacillus plantarum can produce organic acids such as lactic acid, adjust the pH value of the scalp, maintain the acidic environment of the scalp, and be conducive to hair growth. The beneficial bacteria in the composite bacterial agent can inhibit the growth of harmful microorganisms, reduce microbial contamination of the product during storage, and extend the shelf life of the product. The metabolites produced during the fermentation process can enhance the stability of the product, such as lactic acid can adjust the pH value to prevent the product from deteriorating.

[0025] Preferably, in step (2), oxygen is introduced into the enzymatic hydrolyzate to adjust the dissolved oxygen value to 0.5-1.5 mg / L, and fermentation is carried out at 28-35° C. for 1-2 days, and then oxygen is continued to be introduced to adjust the dissolved oxygen value to 4-6 mg / L, and fermentation is continued at 28-35° C. for 1-2 days to obtain a fermentation product.

[0026] The process of aerobic fermentation of the present invention is divided into two steps, the initial stage: adjusting the dissolved oxygen value to 0.5-1.5 mg / L, and fermenting at 28-35°C for 1-2 days; the subsequent stage: continuing to introduce oxygen, adjusting the dissolved oxygen value to 4-6 mg / L, and continuing to ferment at 28-35°C for 1-2 days. In the initial stage, a lower dissolved oxygen value (0.5-1.5 mg / L) can promote the growth of anaerobic bacteria, such as certain lactic acid bacteria, which can produce lactic acid, adjust the pH value, and inhibit the growth of harmful microorganisms. In the subsequent stage, a higher dissolved oxygen value (4-6 mg / L) can promote the growth of aerobic bacteria, such as Bacillus subtilis and Bacillus cereus, which can more efficiently decompose organic matter and produce more beneficial metabolites, such as enzymes, antibacterial substances and organic acids. The present invention can significantly improve the stability of the composition and extend the shelf life of the product through precise control of the dissolved oxygen value, while ensuring the efficacy and safety of the product.

[0027] Preferably, in step (4), the mass ratio of the plant enzymatic fermentation product, the plant extract, the caffeine composition and the oil control composition is (1.5-2.5):(5-8):(0.8-1.2):1.

[0028] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0029] 1. The present invention designs a variety of ingredients in plant raw materials, which can work synergistically to improve the anti-hair loss effect and anti-dandruff effect. Plant raw materials such as ginger root, black ginger root, Alpinia officinalis root and Chuanxiong are rich in a variety of active ingredients, such as gingerol, volatile oil, etc. These ingredients can promote blood circulation, improve the scalp microenvironment, and thus reduce hair loss; plant raw materials such as Sophora flavescens, Cnidium monnieri, Vitex rotundifolia have antifungal and anti-inflammatory effects, and can effectively reduce dandruff and itching.

[0030] 2. The present invention designs multiple components of plant extracts to improve the anti-dandruff and anti-dandruff effects of the composition. Among them, aloe extract, mint extract, forsythia fruit extract, etc. are rich in vitamins, minerals and antioxidants, which can nourish the scalp and hair, promote hair growth, and repair damaged hair; mint extract, forsythia fruit extract, etc. have anti-inflammatory and soothing effects, which can relieve scalp inflammation and itching.

[0031] 3. The present invention can effectively decompose macromolecular substances in plant raw materials through enzymatic hydrolysis and fermentation processes, release more active ingredients, and improve their utilization rate; the metabolites produced during the enzymatic hydrolysis and fermentation process can also enhance the stability of the product and extend the shelf life.

[0032] 4. The plant raw materials and extracts used in the present invention are derived from nature, reducing the use of chemical synthetic ingredients and complying with the concepts of environmental protection and sustainable development. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] Example 1

[0035] This embodiment provides an anti-hair loss and anti-dandruff composition, which is prepared from the following raw materials: plant raw materials, plant extracts, caffeine composition and oil control composition;

[0036] The plant raw materials include the following raw materials by mass: 6 parts of ginger root, 12 parts of black ginger root, 8 parts of Alpinia officinalis root, 2 parts of Chuanxiong, 7.5 parts of Sophora flavescens, 2.4 parts of Cnidium monnieri, 3 parts of Vitex rotundifolia, 4.5 parts of Gleditsia sinensis, 7.5 parts of Sapindus mukorossi, 3 parts of Taraxacum officinale, 8 parts of Polygonum multiflorum, 2 parts of mulberry, 4.8 parts of Platycladus orientalis leaf, 4 parts of mulberry leaf, 8 parts of Portulaca oleracea and 2 parts of Magnolia officinalis;

[0037] Ginger is a plant of the ginger family, Zingiber officinale Roscoe, black ginger is a plant of the kaempferia family, Zingiber officinale Roscoe, and the mountain ginger is a plant of the ginger family, Alpinia japonica (Thunb.) Miq.

[0038] The plant extracts include the following raw materials by weight: 15 parts of aloe extract, 5 parts of peppermint extract, 15 parts of forsythia fruit extract, 10 parts of tea extract, 15 parts of Centella asiatica extract and 5 parts of Cordyceps militaris extract; the above plant extracts are all purchased from Guangzhou Shoumei Cosmetics Co., Ltd.;

[0039] The caffeine composition comprises cyclodextrin and caffeine, and the mass ratio of cyclodextrin to caffeine is 1:1;

[0040] The oil control composition includes disodium azelaic acid, arginine and water, and the mass ratio of disodium azelaic acid, arginine and water is 12:0.5:87.5.

[0041] A method for preparing an anti-dandruff and anti-dandruff composition comprises the following steps:

[0042] (1) mixing plant raw materials according to a ratio, adding water to mix, obtaining a mixed liquid, adding a composite enzymatic hydrolyzing agent and an auxiliary enzymatic hydrolyzing agent to the mixed liquid, performing enzymatic hydrolysis at 32° C. for 6 hours, and then inactivating the enzyme to obtain an enzymatic hydrolysis product;

[0043] The mass ratio of plant raw material to water is 1:18; the composite enzymatic hydrolyzer includes cellulase, papain and trehalase, wherein the amount of cellulase in the mixed liquid is 135 U / mL, the amount of papain in the mixed liquid is 85 U / mL, and the amount of trehalase in the mixed liquid is 120 U / mL; the auxiliary enzymatic hydrolyzer is n-dodecyl alcohol and n-decanol, the mass ratio of n-dodecyl alcohol to n-decanol is 4:1, and the amount of the auxiliary enzymatic hydrolyzer added is 0.2% of the plant raw material;

[0044] Cellulase, papain, and trehalase were purchased from Shanghai Yuanye Biotechnology Co., Ltd., models S10041, S10011, and V13023, respectively;

[0045] (2) adding a composite bacterial agent to the enzymatic hydrolysate, introducing oxygen into the enzymatic hydrolysate to adjust the dissolved oxygen value to 1.0 mg / L, fermenting at 30° C. for 2 days, then continuing to introduce oxygen to adjust the dissolved oxygen value to 5 mg / L, and continuing to ferment at 32° C. for 3 days to obtain a fermentation product;

[0046] The composite bacterial agent includes Lactobacillus geosporum, Bacillus subtilis, Bacillus cereus and Lactobacillus plantarum, wherein the amount of Lactobacillus geosporum in the enzymatic hydrolysate is 4.0×10 9 CFU / mL, the amount of Bacillus subtilis in the enzymatic hydrolysate was 6.0×10 9 CFU / mL, the amount of Bacillus cereus in the enzymatic hydrolysate was 5.0×10 9 CFU / mL, the amount of Lactobacillus plantarum in the enzymatic hydrolysate was 1.4×10 9 CFU / mL;

[0047] Lactobacillus geosporium, Bacillus subtilis, Bacillus cereus and Lactobacillus plantarum were all freeze-dried powders purchased from Shanghai Chen Microbiology Technology Co., Ltd., with models of CICC 10726, GDMCC 1.20, GDMCC 1.842 and CICC23165, respectively. The freeze-dried powders were activated before use, and the activation process was as follows:

[0048] The freeze-dried powders of Lactobacillus geosporum, Bacillus subtilis, Bacillus cereus and Lactobacillus plantarum were added to 35°C water, and then inoculated into the culture medium at an inoculation amount of 8%. Under the conditions of 35°C and 70% humidity, the culture was cultured into a bacterial seed liquid with a bacterial count of 9.5×10 11 CFU / mL, for addition to the enzymatic hydrolysate;

[0049] Culture medium: 20.0 g glucose, 10.0 g yeast extract, 10.0 g peptone, 10.0 g sodium acetate, 5.0 ml salt solution, 1.0 L distilled water, pH 6.8. Salt solution formula: 40.0 g MgSO4·7H2O, 2.0 g MnSO4·4H2O, 2.0 g FeSO4·7H2O, 2.0 g NaCl, 1.0 L distilled water.

[0050] (3) filtering the fermentation product to obtain a supernatant, heating and concentrating the supernatant to obtain a concentrated solution, and freeze-drying the concentrated solution to obtain a plant enzymatic fermentation product;

[0051] (4) Adding a plant extract, a caffeine composition and an oil-control composition to the plant enzymatic fermentation product, wherein the mass ratio of the plant enzymatic fermentation product, the plant extract, the caffeine composition and the oil-control composition is 2:6:1:1, and mixing them evenly to obtain an anti-hair loss and anti-dandruff composition.

[0052] Example 2

[0053] This embodiment provides an anti-hair loss and anti-dandruff composition, which is prepared from the following raw materials: plant raw materials, plant extracts, caffeine composition and oil control composition;

[0054] The plant raw materials include the following raw materials by mass: 6 parts of ginger root, 6 parts of black ginger root, 6 parts of Alpinia officinalis root, 1 part of Chuanxiong, 5 parts of Sophora flavescens, 2.4 parts of Cnidium monnieri, 2 parts of Vitex rotundifolia, 2.4 parts of Gleditsia sinensis, 6 parts of Soapberry peel, 2 parts of Taraxacum officinale, 6 parts of Polygonum multiflorum, 2.4 parts of Mulberry, 4.5 parts of Platycladus orientalis leaf, 3 parts of Mulberry leaf, 7 parts of Portulaca oleracea and 2 parts of Magnolia officinalis;

[0055] The plant extract includes the following raw materials in parts by mass: 16 parts of aloe extract, 8 parts of peppermint extract, 20 parts of forsythia fruit extract, 10 parts of tea extract, 6 parts of Centella asiatica extract and 2 parts of Cordyceps militaris extract;

[0056] The caffeine composition comprises cyclodextrin and caffeine, and the mass ratio of cyclodextrin to caffeine is 1:1;

[0057] The oil control composition includes disodium azelaic acid, arginine and water, and the mass ratio of disodium azelaic acid, arginine and water is 12:0.5:87.5.

[0058] A method for preparing an anti-dandruff and anti-dandruff composition comprises the following steps:

[0059] (1) mixing plant raw materials according to a ratio, adding water to the mixture to obtain a mixed liquid, adding a composite enzymatic hydrolyzer and an auxiliary enzymatic hydrolyzer to the mixed liquid, performing enzymatic hydrolysis at 32° C. for 5 hours, inactivating the enzyme, and obtaining an enzymatic hydrolysis product;

[0060] The mass ratio of plant raw material to water is 1:20; the composite enzymatic hydrolyzer comprises cellulase, papain and trehalase, wherein the amount of cellulase in the mixed liquid is 125 U / mL, the amount of papain in the mixed liquid is 95 U / mL, and the amount of trehalase in the mixed liquid is 120 U / mL; the auxiliary enzymatic hydrolyzer comprises n-dodecyl alcohol and n-decanol, the mass ratio of n-dodecyl alcohol to n-decanol is 3:1, and the amount of the auxiliary enzymatic hydrolyzer added is 0.3% of the plant raw material;

[0061] (2) adding a composite bacterial agent to the enzymatic hydrolysate, introducing oxygen into the enzymatic hydrolysate to adjust the dissolved oxygen value to 1.2 mg / L, fermenting at 30° C. for 2 days, then continuing to introduce oxygen to adjust the dissolved oxygen value to 4.5 mg / L, and continuing to ferment at 35° C. for 2 days to obtain a fermentation product;

[0062] The composite bacterial agent includes Lactobacillus geosporum, Bacillus subtilis, Bacillus cereus and Lactobacillus plantarum, wherein the amount of Lactobacillus geosporum in the enzymatic hydrolysate is 3.8×10 9CFU / mL, the amount of Bacillus subtilis in the enzymatic hydrolysate was 6.2×10 9 CFU / mL, the amount of Bacillus cereus in the enzymatic hydrolysate was 5.3×10 9 CFU / mL, the amount of Lactobacillus plantarum in the enzymatic hydrolysate was 1.2×10 9 CFU / mL;

[0063] The freeze-dried powders of Lactobacillus geosporum, Bacillus subtilis, Bacillus cereus and Lactobacillus plantarum were added to water at 35°C, and then inoculated into the culture medium at an inoculation amount of 8%. The culture was cultured into a bacterial seed liquid at 35°C and a humidity of 70%, and the bacterial content was 1.5×10 12 CFU / mL, for addition to the enzymatic hydrolysate;

[0064] Culture medium: 20.0 g glucose, 10.0 g yeast extract, 10.0 g peptone, 10.0 g sodium acetate, 5.0 ml salt solution, 1.0 L distilled water, pH 6.8. Salt solution formula: 40.0 g MgSO4·7H2O, 2.0 g MnSO4·4H2O, 2.0 g FeSO4·7H2O, 2.0 g NaCl, 1.0 L distilled water.

[0065] (3) filtering the fermentation product to obtain a supernatant, heating and concentrating the supernatant to obtain a concentrated solution, and freeze-drying the concentrated solution to obtain a plant enzymatic fermentation product;

[0066] (4) Adding a plant extract, a caffeine composition and an oil-control composition to the plant enzymatic fermentation product, wherein the mass ratio of the plant enzymatic fermentation product, the plant extract, the caffeine composition and the oil-control composition is 1.8:5.5:0.9:1, and mixing them evenly to obtain an anti-hair loss and anti-dandruff composition.

[0067] Comparative Example 1

[0068] The difference between this comparative example and Example 1 is that the formula of the plant raw materials is different.

[0069] The plant raw materials in this comparative example include the following raw materials by weight:

[0070] 20 parts of ginger roots, 15 parts of black ginger roots, 15 parts of Alpinia officinalis roots, 2 parts of ligusticum chuanxiong, 15 parts of sophora flavescens, 5 parts of cnidium monnieri, 7 parts of vitex rotundifolia, 8 parts of gleditsia sinensis, 10 parts of soapberry peels, 3 parts of dandelions, 10 parts of polygonum multiflorum, 5 parts of mulberries, 8 parts of arborvitae leaves, 4 parts of mulberry leaves, 8 parts of purslane and 2 parts of Magnolia officinalis.

[0071] Comparative Example 2

[0072] The difference between this comparative example and Example 1 is that the formula of the plant extract is different.

[0073] The plant extract in this comparative example includes the following raw materials in parts by mass:

[0074] 15 parts of aloe extract, 15 parts of peppermint extract, 15 parts of forsythia fruit extract, 15 parts of tea extract, 15 parts of Centella asiatica extract and 15 parts of Cordyceps militaris extract.

[0075] Comparative Example 3

[0076] The difference between this comparative example and Example 1 is that the composition of the composite enzymatic hydrolyzing agent is different.

[0077] In this comparative example, papain in the composite enzymolysis agent was replaced with neutral protease, and the neutral protease was purchased from Shanghai Yuanye Biotechnology Co., Ltd., model number S10013.

[0078] Comparative Example 4

[0079] The difference between this comparative example and Example 1 is that the composition of the composite enzymatic hydrolyzing agent is different.

[0080] In this comparative example, the trehalase in the composite enzymatic hydrolyzer was replaced by xylanase, which was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a model number of Y35469.

[0081] Comparative Example 5

[0082] The difference between this comparative example and Example 1 is that the aerobic fermentation process is different.

[0083] The aerobic fermentation process in step (2) of this comparative example is:

[0084] A composite bacterial agent was added to the enzymolysis product, oxygen was introduced into the enzymolysis product to adjust the dissolved oxygen value to 5 mg / L, and the fermentation was continued at 32° C. for 5 days to obtain a fermentation product.

[0085] Comparative Example 6

[0086] The difference between this comparative example and Example 1 is that the types of composite fermentation bacteria agents are different.

[0087] In this comparative example, the Bacillus subtilis in the composite fermentation agent was replaced by Bacillus megaterium, which was purchased from Shanghai Chen Microbiology Technology Co., Ltd., model number GDMCC 1.13.

[0088] Comparative Example 7

[0089] The difference between this comparative example and Example 1 is that the mass ratios of the plant enzymatic hydrolysis and fermentation product, the plant extract, the caffeine composition and the oil-control composition are different.

[0090] In this comparative example, the mass ratio of the plant enzymatic fermentation product, the plant extract, the caffeine composition and the oil control composition is 6:6:1:1.

[0091] Comparative Example 8

[0092] The difference between this comparative example and Example 1 is that no auxiliary enzymatic hydrolysis agent is added in step (1).

[0093] Performance Testing

[0094] The compositions prepared in the examples and comparative examples are prepared into hair compositions, and the specific preparation process is as follows:

[0095] Take an appropriate amount of distilled water and heat it to 85°C. Under stirring conditions, add 12% of fatty alcohol polyoxyethylene ether sodium sulfate (AEO-9), 2% of sodium dodecyl sulfate, 3% of dodecyl dimethylaminoethyl lactone (CAS: 683-10-3), and 0.5% of hexadecanol in sequence, stir until dissolved, cool to 55°C, add 1% of glycerol and 1% of D-panthenol as moisturizers, stir evenly, and when the temperature of the paste drops to room temperature, add 22% of the composition, 0.5% of cystine, 0.3% of zinc sulfate, 0.1% of sodium chloride, 0.1% of pigment, and 0.1% of essence, stir well, make up to 100% with deionized water, and stir to obtain a hair composition.

[0096] (1) Physical and chemical properties test

[0097] The prepared hair composition was tested for physical and chemical indicators according to GB / T 29679-2013, and the test results are shown in Table 1. The test results in Table 1 show that the hair composition prepared in the embodiment meets the standards and reaches the level of excellent products.

[0098] The hair compositions prepared in the examples and comparative examples were placed in a low temperature environment (-30°C) and a high temperature environment (80°C) for 3 days, respectively, and then returned to room temperature, and the physical and chemical indicators were tested again according to the above method. The test results are shown in Table 1.

[0099] After the test, the appearance indicators are scored, where excellent hair composition products have no foreign matter on the appearance and are scored 15 to 20 points; good hair composition products have trace foreign matter on the appearance, which is visible to the naked eye, and are scored 10 to 14 points; poor hair composition products have a small amount of foreign matter on the appearance, which is visible to the naked eye, and are scored 5 to 9 points.

[0100] Table 1 Physical and chemical properties test results

[0101]

[0102]

[0103] It can be seen from Table 1 that the hair composition prepared from the composition of Examples 1-2 can maintain its appearance substantially unaffected after being treated at low and high temperatures, and has good appearance stability.

[0104] (2) Anti-dandruff performance test

[0105] The anti-dandruff performance was tested using the Malassezia inhibition test.

[0106] Test method: Flat plate contact method

[0107] Operation method: remove the hair and fat from the pig skin, cut it into 4cm×4cm square pieces, and sterilize it by Co60-γ ray irradiation.

[0108] Weigh 44.0 g of Leeming-Notman medium base (purchased from Shandong Top Bioengineering Co., Ltd.) in 1 L of deionized water, boil until completely dissolved, add 1.6 mL of olive oil per 80 mL, autoclave and cool to 50 °C, add 40 mg of cycloheximide solution per 80 mL under aseptic operation, and prepare LN plates (90 mm × 15 mm) for use. Add 100 μL of 1 × 10 5 ~3×10 5 CFU / mL of Malassezia suspension is evenly applied and set aside for use.

[0109] After wiping the pig skin surface evenly with 0.5 g of the hair composition sample prepared in Examples 1-2 and Comparative Examples 1-8 for 1 min, the liquid soap on the pig skin was rinsed off with 100 mL of sterile water for 1 min and 2 times; after rinsing, the pig skin was placed in a biosafety cabinet for ventilation and airing until there were no obvious water droplets on the pig skin surface, and then the pig skin was attached to the bacteria-contaminated plate for 15 min. After the attachment was completed, the pig skin was removed, and the plate was placed upright in a (32±2)°C constant temperature box for 48 hours, and the growth of Malassezia was observed, and the antibacterial rate was calculated (the calculation result was rounded to one decimal place). The specific test results are shown in Table 2 below.

[0110] Table 2 Anti-dandruff performance test results

[0111]

[0112]

[0113] As can be seen from Table 2, the hair compositions prepared in Examples 1-2 have obvious antibacterial effects and can better adjust the hair microecology. After 48 hours, the inhibition rate of Malassezia can still reach more than 90%, while the comparative examples cannot maintain a high antibacterial effect for a long time.

[0114] (3) Anti-shedding performance test

[0115] Experimental method: SD rats were half male and half female, and were adaptively fed for 7 days. The rats were randomly divided into a normal control group, a model control group, each example group and each comparison group, with 8 rats in each group. Hair removal was performed on the back area of ​​each rat, and the hair removal area was 9cm×7cm. Except for the normal control group, the rats in the other groups were subcutaneously injected with testosterone propionate (the dosage was 5 mg / kg, the frequency of administration was 1 time / d, and the dosage was halved on the 31st day). The rats in the normal control group were injected with an equal amount of normal saline. The drugs were administered during the modeling process. The rats in each example group were administered with the hair composition of each embodiment in the hair removal area, and the rats in each comparison group were administered with the hair composition of each comparative example in the hair removal area. The drugs were administered once a day for a total of 60 days, and the dosage was 2 mL. The normal control group and the model control group were administered with an equal amount of normal saline in the same manner. After the last administration, the rats were fasted, blood was collected from the artery, and the testosterone (T) and estradiol (E2) content in the serum were detected by ELISA. Blood was collected from the abdominal cavity, and the skin of the hair loss part on the back of the rats was removed, homogenized with PBS and centrifuged, and the supernatant was taken and the content of hepatocyte growth factor (HGF) was detected by ELISA. The test results are shown in Table 3.

[0116] Table 3 Anti-slip performance test results

[0117]

[0118]

[0119] There is a significant correlation between seborrheic alopecia and sex hormones in the body and cell growth factors in hair follicles. Therefore, the levels of testosterone (T) and estradiol (E2) and cell growth factor (HGF) in rats were detected. It can be seen from Table 3 that the hair composition of Examples 1-2 can effectively reduce serum testosterone levels, while increasing estradiol and hepatocyte growth factor levels, and has a good anti-hair loss effect.

[0120] It can be seen from the above performance test results that Examples 1-2 have good appearance stability and anti-dandruff and anti-dandruff effects, especially Example 1 has the most outstanding comprehensive performance. This is mainly because the present invention performs a specific enzymatic hydrolysis and fermentation process on the plant raw materials, and also designs the composition and ratio of the plant raw materials and plant extracts, thereby improving the stability and anti-dandruff and anti-dandruff effects of the hair composition.

[0121] The comparative examples, however, were significantly inferior to the examples in the corresponding performance tests because they did not adopt the necessary technical solutions. In comparative examples 1 and 2, the raw material ratios of plant raw materials and plant extracts were changed respectively. From the results, it can be seen that the stability and anti-dandruff and anti-dandruff effects of the hair compositions prepared therefrom were deteriorated, proving that the composition and ratio of plant raw materials and the composition and ratio of plant extracts had an important influence on the comprehensive effects of the hair compositions. In comparative examples 3 and 4, the type of one enzymatic agent in the composite enzymatic agent was changed respectively. From the results, it can be seen that the appearance stability and anti-dandruff and anti-dandruff effects of the hair compositions were affected, and the comprehensive performance decreased, indicating that the composition of the composite enzymatic agent in the present invention had an important influence on the comprehensive performance of the composition. In comparative example 5, the aerobic fermentation process was changed, and the process of fermentation at a specific dissolved oxygen value in two stages was not adopted. From the results, it can be seen that the stability and anti-dandruff and anti-dandruff performance of the hair compositions prepared therefrom decreased to varying degrees, indicating that the two-step oxygenation and fermentation at a specific dissolved oxygen value in the present invention have an important influence on improving the comprehensive performance of the hair compositions. In Comparative Example 6, the type of a fermentation agent in the composite fermentation agent was changed. From the results, it can be seen that the comprehensive performance of the hair composition prepared by it decreased, indicating that the use of a specific type of fermentation agent in the present invention has a very important influence on the performance of the hair composition. In Comparative Example 7, the mass ratio of the plant enzymatic fermentation product, the plant extract, the caffeine composition and the oil control composition in the composition was changed. From the results, it can be seen that this will also affect the anti-dandruff and stability of the hair composition, which shows that the setting of the above mass ratio in the present invention has an important influence on improving the comprehensive performance of the hair composition. In Comparative Example 8, no auxiliary enzymatic agent was added during the enzymatic hydrolysis process. From the results, it can be seen that the stability and anti-dandruff and anti-dandruff effects of the hair composition prepared by it decreased, indicating that the composite enzymatic agent and the auxiliary enzymatic agent are used together, and the two have a synergistic effect, which improves the enzymatic hydrolysis process of the plant raw material and releases more effective ingredients, which has an important influence on improving the stability and anti-dandruff and anti-dandruff effects of the hair composition. The above experimental results further prove the importance of the technical scheme defined in the present invention for its technical effects.

[0122] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An anti-dandruff and anti-dandruff composition, characterized in that: The anti-hair loss and anti-dandruff composition is prepared from the following raw materials: plant raw materials, plant extracts, caffeine composition and oil control composition; The plant raw materials include the following raw materials by weight: 5-20 parts of ginger root, 5-20 parts of black ginger root, 5-20 parts of Alpinia officinalis root, 1-3 parts of Chuanxiong, 5-10 parts of Sophora flavescens, 2-4 parts of Cnidium monnieri, 1-5 parts of Vitex rotundifolia, 3-5 parts of Gleditsia sinensis, 5-8 parts of Sapindus mukorossi, 1-5 parts of Taraxacum officinale, 5-8 parts of Polygonum multiflorum, 1-3 parts of mulberry, 3-6 parts of Platycladus orientalis leaf, 3-6 parts of mulberry leaf, 7-10 parts of Portulaca oleracea and 1-4 parts of Magnolia officinalis; The plant extract includes the following raw materials in parts by mass: 5-20 parts of aloe extract, 1-10 parts of peppermint extract, 5-20 parts of forsythia fruit extract, 5-20 parts of tea extract, 5-20 parts of Centella asiatica extract and 1-10 parts of Cordyceps militaris extract; The caffeine composition includes cyclodextrin and caffeine; The oil control composition includes disodium azelaate, arginine and water.

2. The anti-dandruff and anti-dandruff composition according to claim 1, characterized in that: The mass ratio of ginger root, black ginger root, Alpinia officinalis root and Ligusticum chuanxiong is (2-6):(2-6):(2-6):

1.

3. The anti-dandruff and anti-dandruff composition according to claim 1, characterized in that: The mass ratio of Sophora flavescens, Cnidium monnieri, Vitex rotundifolia, Gleditsia sinensis, Sapindus mukorossi and Taraxacum officinale is (1.5-2.5):(0.8-1.2):1:(1-1.5):(2-3):

1.

4. The anti-dandruff and anti-dandruff composition according to claim 1, characterized in that: The mass ratio of Polygonum multiflorum, mulberry, Platycladus orientalis leaf and mulberry leaf is (1.5-2.5):(0.5-0.8):(1-1.5):

1.

5. The anti-dandruff and anti-dandruff composition according to claim 1, characterized in that: The mass ratio of aloe vera extract to peppermint extract is (2-3.5):1, the mass ratio of forsythia fruit extract to tea extract is (1-2.5):1, and the mass ratio of Centella asiatica extract to Cordyceps militaris extract is (3-4.5):

1.

6. A method for preparing an anti-dandruff and anti-dandruff composition according to any one of claims 1 to 5, characterized in that: The following steps are involved: (1) mixing plant raw materials according to a ratio, adding water to mix, obtaining a mixed liquid, adding a composite enzymatic hydrolyzing agent and an auxiliary enzymatic hydrolyzing agent to the mixed liquid, performing enzymatic hydrolysis at 30 to 38° C. for 5 to 8 hours, inactivating the enzyme, and obtaining an enzymatic hydrolysis product; (2) adding a composite bacterial agent to the enzymatic hydrolyzate, introducing oxygen, fermenting at 28 to 35° C. for 3 to 5 days, sterilizing, and obtaining a fermentation product; (3) filtering the fermentation product to obtain a supernatant, heating and concentrating the supernatant to obtain a concentrated solution, and freeze-drying the concentrated solution to obtain a plant enzymatic fermentation product; (4) Adding the plant extract, the caffeine composition and the oil control composition to the plant enzymatic fermentation product and mixing them evenly to obtain an anti-hair loss and anti-dandruff composition.

7. The method for preparing the anti-dandruff and anti-dandruff composition according to claim 6, characterized in that: In step (1), the mass ratio of plant raw material to water is 1:(15-20); the composite enzymatic hydrolyzer comprises cellulase, papain and trehalase, wherein the amount of cellulase in the mixed liquid is 100-150 U / mL, the amount of papain in the mixed liquid is 80-100 U / mL, and the amount of trehalase in the mixed liquid is 110-130 U / mL; the auxiliary enzymatic hydrolyzer comprises n-dodecyl alcohol and n-decanol, the mass ratio of n-dodecyl alcohol to n-decanol is (3-5):1, and the amount of the auxiliary enzymatic hydrolyzer added is 0.1-0.3% of the plant raw material.

8. The method for preparing the anti-dandruff and anti-dandruff composition according to claim 6, characterized in that: In step (2), the composite bacterial agent includes Lactobacillus geosporum, Bacillus subtilis, Bacillus cereus and Lactobacillus plantarum, wherein the amount of Lactobacillus geosporum in the enzymatic hydrolysate is 3.5×10 9 ~4.2×10 9 CFU / mL, the amount of Bacillus subtilis in the enzymatic hydrolysate was 5.8×10 9 ~6.5×10 9 CFU / mL, the amount of Bacillus cereus in the enzymatic hydrolysate was 4.5×10 9 ~5.5×10 9 CFU / mL, the amount of Lactobacillus plantarum in the enzymatic hydrolysate was 1.2×10 9 ~1.5×10 9 CFU / mL.

9. The method for preparing an anti-dandruff and anti-dandruff composition according to claim 6, characterized in that: In step (2), oxygen is introduced into the enzymatic hydrolyzate to adjust the dissolved oxygen value to 0.5-1.5 mg / L, and the product is fermented at 28-35° C. for 1-2 days. Subsequently, oxygen is continued to be introduced to adjust the dissolved oxygen value to 4-6 mg / L, and the product is fermented at 28-35° C. for 1-3 days to obtain a fermentation product.

10. The method for preparing the anti-dandruff and anti-dandruff composition according to claim 6, characterized in that: In step (4), the mass ratio of the plant enzymatic fermentation product, the plant extract, the caffeine composition and the oil control composition is (1.5-2.5):(5-8):(0.8-1.2):1.

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