Preparation for improving intestinal environment and preparation method thereof

Abstract

The invention relates to a preparation for improving the intestinal environment. The preparation is mainly prepared from the following raw materials by weight: 5-10 g of bighead atractylodes rhizome, 5-10 g of poria cocos, 5-10 g of hawthorn, 5-10 g of eucommia ulmoides, 3-5 g of wormwood, 3-5 g of astragalus membranaceus, 3-5 g of angelica sinensis, 3-5 g of butyric acid, 5-8 g of vitamin C, 10-15 g of garlic and 5-7 g of probiotics. The raw materials of the preparation are reasonably compatible, respective unique action mechanisms are exerted, beneficial bacteria are activated, harmful bacteria are inhibited, gastrointestinal flora balance is regulated, and intestinal health of pigs is synergistically improved.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and mainly relates to a preparation for improving intestinal environment and a preparation method thereof. Background Art

[0002] In modern pig farming, the intestinal health of pigs has a vital impact on their growth performance, immunity and disease resistance. A poor intestinal environment can easily lead to digestion and absorption disorders in pigs and increase susceptibility to diseases, thus causing huge economic losses to the farming industry. Existing drugs to improve the intestinal environment mostly use Western medicines, which are prone to drug resistance and have serious side effects.

[0003] Atractylodes macrocephala has the effect of strengthening the spleen and replenishing qi. It can regulate the gastrointestinal function of pigs and enhance the digestion and absorption capacity of the intestines.

[0004] Poria cocos can promote diuresis and eliminate dampness, strengthen the spleen and calm the mind. It helps maintain the stability of the intestinal environment and promote the growth of beneficial bacteria.

[0005] Hawthorn, rich in various organic acids, can promote the secretion of gastric juice in pigs, enhance the activity of digestive enzymes, improve the digestive function of the intestines, reduce the retention time of food in the intestines, and reduce the risk of intestinal diseases.

[0006] Eucommia ulmoides has the function of nourishing the liver and kidneys, strengthening the tendons and bones, and is beneficial to improving the overall immunity of pigs, indirectly ensuring the health of the intestines.

[0007] Mugwort contains volatile oils and other ingredients, which have antibacterial and anti-inflammatory effects. It can inhibit the reproduction of harmful bacteria in the intestines and maintain the balance of intestinal flora.

[0008] Astragalus can replenish qi and raise yang, consolidate the exterior and stop sweating, enhance the pig's physique and resistance, and make the intestines more capable of resisting adverse external influences.

[0009] Angelica sinensis has the effect of nourishing blood and activating blood circulation, providing sufficient nutrition and oxygen to intestinal cells and promoting the repair and renewal of intestinal mucosa.

[0010] Butyric acid, as a short-chain fatty acid, is an important source of energy for intestinal cells. It can maintain the integrity of the intestinal mucosa, enhance the intestinal barrier function, and reduce the damage of harmful substances to the intestine.

[0011] Vitamin C can promote the absorption of iron in the intestine, help prevent and treat iron deficiency anemia, and reduce the permeability and fragility of capillaries, thereby reducing intestinal bleeding and inflammation. Vitamin C can also improve intestinal barrier function and inhibit the growth of harmful bacteria by regulating the composition of intestinal microbiota, thereby maintaining intestinal health.

[0012] Garlic, whose main ingredients include allicin, sulfur compounds, vitamin C, vitamin B6 and selenium, has multiple functions such as antibacterial, antiviral, anti-inflammatory and antioxidant. It can enhance immunity, promote digestion and improve gastrointestinal function.

[0013] The invention uses a reasonable combination of raw materials to exert their own unique mechanisms of action, activate beneficial bacteria, inhibit harmful bacteria, regulate the balance of gastrointestinal flora, and synergistically improve the intestinal health of pigs. At the same time, the process of coating after the garlic juice is fermented with Chinese medicine not only improves the bioavailability and stability of the drug, but also makes it easy to use. ， All the green raw materials used are residue-free and drug-resistant, which are safe and effective. Summary of the invention

[0014] The purpose of the present invention is to overcome the defects of the prior art and provide a preparation for improving the intestinal environment. The raw materials of the preparation are reasonably matched to exert their own unique mechanism of action, activate beneficial bacteria, inhibit harmful bacteria, regulate the balance of gastrointestinal flora, and synergistically improve the intestinal health of pigs.

[0015] The second object of the present invention is to provide a method for preparing the above-mentioned preparation for improving the intestinal environment, which adopts the process of fermenting the traditional Chinese medicine with garlic juice and then coating, which not only improves the bioavailability and stability of the drug, but also makes it easy to use.

[0016] The third object of the present invention is to provide the effects of the above-mentioned preparation on pig production performance and microbial flora in feces.

[0017] To achieve the above object, the present invention adopts the following technical solution: A preparation for improving the intestinal environment, which is mainly made of the following raw materials in weight ratio: Atractylodes macrocephala 5-10g, Poria cocos 5-10g, Hawthorn 5-10g, Eucommia ulmoides 5-10g, Artemisia annua 3-5g, Astragalus 3-5g, Angelica 3-5g, Butyric acid 3-5g, Vitamin C 5-8g, Garlic 10-15g, Probiotics 5-7g.

[0018] Specifically, the probiotics may be one or a combination of two of Bacillus subtilis, Lactobacillus, etc.

[0019] The preparation for improving the intestinal environment of the present invention can also be mainly made of the following raw materials in the following weight ratio: 5g of Atractylodes macrocephala, 10g of Poria cocos, 5g of Crataegus pinnatifida, 10g of Eucommia ulmoides, 3g of Artemisia argyi, 5g of Astragalus membranaceus, 3g of Angelica sinensis, 5g of butyric acid, 5g of Vitamin C, 15g of garlic, and 5g of Bacillus subtilis.

[0020] The preparation for improving the intestinal environment of the present invention can also be mainly made of the following raw materials in the following weight ratio: 10g of Atractylodes macrocephala, 5g of Poria cocos, 10g of Hawthorn, 5g of Eucommia ulmoides, 5g of Artemisia argyi, 3g of Astragalus, 5g of Angelica sinensis, 3g of butyric acid, 8g of Vitamin C, 10g of garlic, and 7g of Lactobacillus.

[0021] The preparation for improving the intestinal environment of the present invention can also be mainly made of the following raw materials in the following weight ratio: 7.5g of Atractylodes macrocephala, 7.5g of Poria cocos, 7.5g of Crataegus pinnatifida, 7.5g of Eucommia ulmoides, 4g of Artemisia argyi, 4g of Astragalus membranaceus, 4g of Angelica sinensis, 4g of butyric acid, 6.5g of Vitamin C, 12.5g of Garlic, 3g of Bacillus subtilis, and 3g of Lactobacillus acidophilus.

[0022] The present invention provides a method for preparing the above-mentioned preparation for improving the intestinal environment, which comprises the following steps: (1) Peel the garlic and crush it, add appropriate amount of salt and water to stir into garlic paste, and then separate the solid and liquid to obtain garlic juice and garlic residue respectively, and set aside; (2) Take Atractylodes macrocephala, Poria cocos, Crataegus pinnatifida, Eucommia ulmoides, Artemisia argyi, Astragalus membranaceus, and Angelica sinensis, grind them, add vitamin C and garlic residue, mix well, and obtain mixed material 2; (3) Add probiotics and butyric acid to garlic juice, mix well, add to mixture 2, mix well, place in a fermentation tank, and ferment at 30-35° C. for 60-84 hours to obtain mixture 3 (i.e., fermentation liquid); (4) Dissolve β-cyclodextrin in an appropriate amount of water and heat to 40-50°C to obtain a coating solution for later use; (5) Add mixed material 3 to the coating solution, mix well (mixing can be done by stirring evenly, keeping the temperature and stirring speed constant for 1-2 hours), and spray dry to obtain.

[0023] Specifically, in step (1), the amount of water used can be 8-12 times the mass of the garlic; and the amount of salt used can be 0.1-0.2 times the mass of the garlic.

[0024] Specifically, in step (4), the mass ratio of β-cyclodextrin to water can be 1:2-3, and the amount of β-cyclodextrin can be 10-15% of the mass of the mixture 3 (i.e., the fermentation liquid).

[0025] The present invention also provides a method for using the above-mentioned preparation for improving the intestinal environment, specifically: every 400-500g of the preparation can be mixed with 100kg of feed or 200kg of water, and can be used for a long time.

[0026] The present invention also provides an application of the above-mentioned preparation in improving the intestinal environment of livestock and poultry, especially the effect on the production performance of pigs and the microbial flora in feces.

[0027] Compared with the prior art, the present invention has the following advantages and beneficial effects: (1) The present invention can activate beneficial bacteria (such as lactobacillus), inhibit harmful bacteria (such as Escherichia coli), regulate the balance of gastrointestinal flora, and synergistically improve the intestinal health of pigs. At the same time, it can enhance the body's immunity and disease resistance and reduce the occurrence of diseases; (2) The present invention uses garlic juice to ferment traditional Chinese medicine and then coat it, which not only improves the bioavailability of the drug, but also improves the quality stability of the drug; (3) The process of the present invention is suitable for industrial production, is easily absorbed after entering the body, and is convenient for clinical administration, suitable for group administration, and saves manpower and material resources; (4) The drug content of the present invention is clear, the dosage is accurate, and the quality is controllable. DETAILED DESCRIPTION

[0028] The technical solution of the present invention is further described in detail below in conjunction with the embodiments, but the protection scope of the present invention is not limited thereto.

[0029] In the following examples, unless otherwise specified, the raw materials used are all common commercial products that can be directly purchased or can be prepared by conventional methods in the art. For example, Bacillus subtilis (viable count ≥ 100 billion CFU / g) was purchased from Luoyang Oukebaik Biotechnology Co., Ltd. Lactobacillus (viable count ≥ 10 billion CFU / g) was purchased from Shandong Pingju Biotechnology Co., Ltd.

[0030] Example 1. Preparation of a preparation for improving intestinal environment Its components and their weights are: 5g of Atractylodes macrocephala, 10g of Poria cocos, 5g of Crataegus pinnatifida, 10g of Eucommia ulmoides, 3g of Artemisia argyi, 5g of Astragalus membranaceus, 3g of Angelica sinensis, 5g of butyric acid, 5g of Vitamin C, 15g of Garlic, and 5g of Bacillus subtilis.

[0031] Preparation method: (1) Peel and mash fresh garlic, add appropriate amount of salt and purified water to make garlic paste, squeeze out the garlic juice through gauze and set aside, and set aside the garlic residue; (2) Take Atractylodes macrocephala, Poria cocos, Crataegus pinnatifida, Eucommia ulmoides, Artemisia argyi, Astragalus membranaceus, and Angelica sinensis, grind them into 80 meshes, add vitamin C and garlic residue, mix well, and obtain mixed material 2; (3) Add Bacillus subtilis and butyric acid to garlic juice, mix well, add to mixture 2, mix well, place in a fermentation tank, and ferment at 30-35° C. for 72 hours to obtain mixture 3 (i.e., fermentation liquid); (4) Dissolve β-cyclodextrin in an appropriate amount of purified water (the mass ratio of β-cyclodextrin to purified water is 1:2.5), heat to 40-50°C to obtain a coating solution for later use; (5) Slowly add the mixture 3 to the coating solution and stir evenly (keep the temperature and stirring speed constant for 1.5 hours). After the coating is completed, dry it by spray drying to obtain the product of the present invention.

[0032] In this method: (1) in step 1, the amount of purified water used is 10 times the mass of the garlic; the amount of salt used is one tenth the mass of the garlic; (2) The amount of β-cyclodextrin used is 10% of the total mass of the fermentation broth.

[0033] Example 2. Preparation of a preparation for improving intestinal environment Its components and their weights are: 10g of Atractylodes macrocephala, 5g of Poria cocos, 10g of Crataegus pinnatifida, 5g of Eucommia ulmoides, 5g of Artemisia argyi, 3g of Astragalus membranaceus, 5g of Angelica sinensis, 3g of butyric acid, 8g of Vitamin C, 10g of Garlic, and 7g of Lactobacillus acidophilus.

[0034] Preparation method: (1) Peel and mash fresh garlic, add appropriate amount of salt and purified water to make garlic paste, squeeze out the garlic juice through gauze and set aside, and set aside the garlic residue; (2) Take Atractylodes macrocephala, Poria cocos, Crataegus pinnatifida, Eucommia ulmoides, Artemisia argyi, Astragalus membranaceus, and Angelica sinensis, grind them into 80 meshes, add vitamin C and garlic residue, mix well, and obtain mixed material 2; (3) Add lactobacillus and butyric acid to garlic juice, mix well, add to mixture 2, mix well, place in a fermentation tank, and ferment at 30-35° C. for 72 hours to obtain mixture 3 (i.e., fermentation liquid); (4) Dissolve β-cyclodextrin in an appropriate amount of purified water (the mass ratio of β-cyclodextrin to purified water is 1:2.5), heat to 40-50°C to obtain a coating solution for later use; (5) Slowly add the mixture 3 to the coating solution and stir evenly (keep the temperature and stirring speed constant for 1.5 hours). After the coating is completed, dry it by spray drying to obtain the product of the present invention.

[0035] In this method: (1) in step 1, the amount of purified water used is 10 times the mass of the garlic; the amount of salt used is one tenth the mass of the garlic; (2) The amount of β-cyclodextrin used is 15% of the total mass of the fermentation broth.

[0036] Example 3. Preparation of a preparation for improving intestinal environment Its components and their weights are: 7.5g of Atractylodes macrocephala, 7.5g of Poria cocos, 7.5g of Crataegus pinnatifida, 7.5g of Eucommia ulmoides, 4g of Artemisia argyi, 4g of Astragalus membranaceus, 4g of Angelica sinensis, 4g of butyric acid, 6.5g of Vitamin C, 12.5g of Garlic, 3g of Bacillus subtilis, and 3g of Lactobacillus acidophilus.

[0037] Preparation method: (1) Peel and mash fresh garlic, add appropriate amount of salt and purified water to make garlic paste, squeeze out the garlic juice through gauze and set aside, and set aside the garlic residue; (2) Take Atractylodes macrocephala, Poria cocos, Crataegus pinnatifida, Eucommia ulmoides, Artemisia argyi, Astragalus membranaceus, and Angelica sinensis, grind them into 80 meshes, add vitamin C and garlic residue, mix well, and obtain mixed material 2; (3) Add Bacillus subtilis, Lactobacillus acidobacillus and butyric acid to garlic juice, mix well, add to mixture 2, mix well, place in a fermentation tank, and ferment at 30-35° C. for 72 hours to obtain mixture 3 (i.e., fermentation liquid); (4) Dissolve β-cyclodextrin in an appropriate amount of purified water (the mass ratio of β-cyclodextrin to purified water is 1:2.5), heat to 40-50°C to obtain a coating solution for later use; (5) Slowly add the mixture 3 to the coating solution and stir evenly (keep the temperature and stirring speed constant for 1.5 hours). After the coating is completed, dry it by spray drying to obtain the product of the present invention.

[0038] In this method: (1) in step 1, the amount of purified water used is 10 times the mass of the garlic; the amount of salt used is one tenth the mass of the garlic; (2) The amount of β-cyclodextrin used is 12% of the total mass of the fermentation broth.

[0039] Comparative Example 1. Preparation of a reference substance for a preparation for improving the intestinal environment Its components and their weights are: 7.5g of Atractylodes macrocephala, 7.5g of Poria cocos, 7.5g of Crataegus pinnatifida, 7.5g of Eucommia ulmoides, 4g of Artemisia argyi, 4g of Astragalus membranaceus, 4g of Angelica sinensis, 4g of butyric acid, 6.5g of Vitamin C, 12.5g of Garlic, 3g of Bacillus subtilis, and 3g of Lactobacillus acidophilus.

[0040] Preparation method: (1) Peel and mash fresh garlic, add appropriate amount of salt and purified water to make garlic paste, squeeze out the garlic juice through gauze and set aside, and set aside the garlic residue; (2) Take Atractylodes macrocephala, Poria cocos, Crataegus pinnatifida, Eucommia ulmoides, Artemisia argyi, Astragalus membranaceus, and Angelica sinensis, grind them into 80 meshes, add vitamin C and garlic residue, mix well, and obtain mixed material 2; (3) Add Bacillus subtilis, Lactobacillus and butyric acid to garlic juice, mix well, add to mixed material 2, mix well, place in a fermentation tank, and ferment at 30-35°C for 72 hours. Take out, dry at low temperature (below 35°C), and crush to obtain.

[0041] In the method, the amount of purified water used in step 1 is 10 times the mass of the garlic; the amount of salt used is one tenth the mass of the garlic.

[0042] Note: This method does not use coating process.

[0043] Comparative Example 2. Preparation of a reference substance for a preparation for improving the intestinal environment Its components and their weights are: 7.5g of Atractylodes macrocephala, 7.5g of Poria cocos, 7.5g of Crataegus pinnatifida, 7.5g of Eucommia ulmoides, 4g of Artemisia argyi, 4g of Astragalus membranaceus, 4g of Angelica sinensis, 4g of butyric acid, 6.5g of Vitamin C, 12.5g of Garlic, 3g of Bacillus subtilis, and 3g of Lactobacillus acidophilus.

[0044] Preparation method: (1) Take fresh garlic, Atractylodes macrocephala, Poria cocos, hawthorn, Eucommia ulmoides, Artemisia argyi, Astragalus membranaceus, and Angelica sinensis, grind into 80 mesh, add vitamin C, mix well, and obtain a mixture 1; (3) Add Bacillus subtilis, Lactobacillus acidobacillus and butyric acid to purified water, mix well, add to mixture 1, mix well, place in a fermentation tank, and ferment at 30-35° C. for 72 hours to obtain mixture 3 (i.e., fermentation liquid); (4) Dissolve β-cyclodextrin in an appropriate amount of purified water (the mass ratio of β-cyclodextrin to purified water is 1:2.5), heat to 40-50°C to obtain a coating solution for later use; (5) Slowly add the mixture 3 to the coating solution and stir evenly (keep the temperature and stirring speed constant for 1.5 hours). After the coating is completed, dry it by spray drying to obtain the product of the present invention.

[0045] In this method: (1) The amount of β-cyclodextrin used is 12% of the total mass of the fermentation broth.

[0046] Note: This method does not use the method of garlic juice fermentation.

[0047] Application example: Effects on piglet production performance and fecal microbial flora 1. Drugs and reagents Drugs in the test group: the compound preparations prepared in Examples 1, 2, and 3 were added respectively; Control group drugs: add the compound preparations prepared in Comparative Examples 1 and 2 respectively; Blank control group: no drug was added.

[0048] (II) Test methods (1) Experimental design: 180 21-day-old weaned piglets of similar parity, weight and breed were selected as experimental animals and randomly divided into 6 groups, 30 pigs in each group, including 3 experimental groups, 2 control groups and 1 blank control group. Free feeding, epidemic prevention and management measures were adopted in the same way as usual, and the experimental period was 10 days.

[0049] Here’s how to add it: 3 experimental groups: mixed feeding, 500g mixed with 100kg feed, for 10 consecutive days.

[0050] 2 control groups: mixed feeding, 500g mixed with 100kg feed, for 10 consecutive days.

[0051] Blank control group: no drugs were added and the mice were fed freely.

[0052] The observation and recording items include: initial body weight, final body weight, daily weight gain, average feed consumption, and feed-to-meat ratio.

[0053] On the first and tenth days of the experiment, five piglets were randomly selected from each replicate, and fresh feces samples were taken to culture and count the number of Escherichia coli and Lactobacillus in the fresh feces.

[0054] (2) Results and analysis: See Tables 1 and 2.

[0055] Table 1 Comparison of pig production performance As can be seen from Table 1, the average daily weight gain of experimental groups 1, 2, and 3 was 499g, which was 5.7%, 7.8%, and 14.7% higher than that of control groups 1, 2, and the blank control group, respectively. The average feed-to-meat ratio of experimental groups 1, 2, and 3 was 1.75, which was 4.9%, 6.9%, and 11.2% lower than that of control groups 1, 2, and the blank control group, respectively.

[0056] Table 2 Microbial counts in feces (CFU / g) As can be seen from Table 2, the reduction in the number of Escherichia coli in the feces of the experimental groups 1, 2, and 3 was higher than that of the control group, and was significantly higher than that of the blank control group, and the increase in the number of Lactobacillus was higher than that of the control group, and was significantly higher than that of the blank control group.

[0057] It can be seen that the garlic juice fermentation and coating process used in the preparation of the present invention can improve the bioavailability of the drug, and the adopted formula is reasonable, which can well improve the intestinal health of pigs and improve the production performance of pigs.

Claims

1. A preparation for improving intestinal environment, characterized in that: It is mainly made of the following raw materials in weight ratio: Atractylodes macrocephala 5-10g, Poria cocos 5-10g, Hawthorn 5-10g, Eucommia ulmoides 5-10g, Artemisia annua 3-5g, Astragalus 3-5g, Angelica 3-5g, Butyric acid 3-5g, Vitamin C 5-8g, Garlic 10-15g, Probiotics 5-7g.

2. The preparation for improving intestinal environment according to claim 1, characterized in that: The probiotics are one or a combination of Bacillus subtilis and Lactobacillus.

3. The preparation for improving intestinal environment according to claim 2, characterized in that: The invention is mainly prepared from the following raw materials in the following weight proportions: 5g of Atractylodes macrocephala, 10g of Poria cocos, 5g of Crataegus pinnatifida, 10g of Eucommia ulmoides, 3g of Artemisia argyi, 5g of Astragalus membranaceus, 3g of Angelica sinensis, 5g of butyric acid, 5g of Vitamin C, 15g of Garlic and 5g of Bacillus subtilis.

4. The preparation for improving intestinal environment according to claim 2, characterized in that: The invention is mainly prepared from the following raw materials in the following weight ratio: 10g of Atractylodes macrocephala, 5g of Poria cocos, 10g of Hawthorn, 5g of Eucommia ulmoides, 5g of Artemisia argyi, 3g of Astragalus, 5g of Angelica sinensis, 3g of butyric acid, 8g of Vitamin C, 10g of Garlic and 7g of Lactobacillus.

5. The preparation for improving intestinal environment according to claim 2, characterized in that: The invention is mainly prepared from the following raw materials in the following weight ratio: 7.5g of Atractylodes macrocephala, 7.5g of Poria cocos, 7.5g of Hawthorn, 7.5g of Eucommia ulmoides, 4g of Artemisia argyi, 4g of Astragalus, 4g of Angelica sinensis, 4g of butyric acid, 6.5g of Vitamin C, 12.5g of Garlic, 3g of Bacillus subtilis and 3g of Lactobacillus acidophilus.

6. The method for preparing the preparation for improving the intestinal environment according to any one of claims 1 to 5, characterized in that: The steps include: (1) Peel the garlic and crush it, add appropriate amount of salt and water to stir into garlic paste, and then separate the solid and liquid to obtain garlic juice and garlic residue respectively, and set aside; (2) Take Atractylodes macrocephala, Poria cocos, Crataegus pinnatifida, Eucommia ulmoides, Artemisia argyi, Astragalus membranaceus, and Angelica sinensis, grind them, add vitamin C and garlic residue, mix well, and obtain mixed material 2; (3) Add probiotics and butyric acid to garlic juice, mix well, add to mixture 2, mix well, and ferment at 30-35° C. for 60-84 hours to obtain mixture 3; (4) Dissolve β-cyclodextrin in an appropriate amount of water and heat to 40-50°C to obtain a coating solution for later use; (5) Add mixed material 3 to the coating solution, mix well, and spray dry to obtain.

7. The method for preparing the preparation for improving the intestinal environment according to claim 6, characterized in that: In step (1), the amount of water used is 8-12 times the mass of the garlic; and the amount of salt used is 0.1-0.2 times the mass of the garlic.

8. The method for preparing the preparation for improving the intestinal environment according to claim 6, characterized in that: In step (4), the mass ratio of β-cyclodextrin to water is 1:2-3, and the amount of β-cyclodextrin used is 10-15% of the mass of mixture 3.

9. The method for using the preparation for improving intestinal environment according to any one of claims 1 to 5, characterized in that: For every 400-500g of preparation, mix with 100kg of feed or add 200kg of water.

10. Use of the preparation according to any one of claims 1 to 5 in improving the intestinal environment of livestock and poultry.

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